LOCUS       DACCCM010000001        93453 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCCM010000001 DACCCM010000000
VERSION     DACCCM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.001"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            83..970
                     /locus_tag="IVK78_000001"
     CDS             83..970
                     /locus_tag="IVK78_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6373509.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IVK78_000002"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IVK78_000002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP6373510.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IVK78_000003"
     CDS             2272..2718
                     /locus_tag="IVK78_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6373511.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IVK78_000004"
     CDS             2991..4343
                     /locus_tag="IVK78_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP6373512.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IVK78_000005"
     CDS             4514..4801
                     /locus_tag="IVK78_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373513.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IVK78_000006"
     CDS             5027..5470
                     /locus_tag="IVK78_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6373514.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IVK78_000007"
     CDS             complement(5476..6660)
                     /locus_tag="IVK78_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HAP6373515.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGCAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IVK78_000008"
     CDS             6928..7626
                     /locus_tag="IVK78_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP6373516.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IVK78_000009"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IVK78_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP6373517.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IVK78_000010"
     CDS             complement(8436..8969)
                     /locus_tag="IVK78_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373518.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IVK78_000011"
     CDS             complement(9266..11647)
                     /locus_tag="IVK78_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6373519.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVK78_000012"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVK78_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP6373520.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IVK78_000013"
     CDS             12348..12887
                     /locus_tag="IVK78_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP6373521.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IVK78_000014"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IVK78_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP6373522.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IVK78_000015"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVK78_000015"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IVK78_000016"
     CDS             13994..15160
                     /locus_tag="IVK78_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP6373523.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IVK78_000017"
     CDS             15206..16702
                     /locus_tag="IVK78_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP6373524.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IVK78_000018"
     CDS             16783..17466
                     /locus_tag="IVK78_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6373525.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IVK78_000019"
     CDS             18111..19301
                     /locus_tag="IVK78_000019"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP6373526.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IVK78_000020"
     CDS             19403..20356
                     /locus_tag="IVK78_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP6373527.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IVK78_000021"
     CDS             20425..21774
                     /locus_tag="IVK78_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6373528.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IVK78_000022"
     CDS             complement(21957..22280)
                     /locus_tag="IVK78_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373529.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IVK78_000023"
     CDS             22409..23572
                     /locus_tag="IVK78_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373530.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IVK78_000024"
     CDS             23642..24991
                     /locus_tag="IVK78_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6373531.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IVK78_000025"
     CDS             complement(25036..25467)
                     /locus_tag="IVK78_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6373532.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IVK78_000026"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IVK78_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP6373533.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IVK78_000027"
     CDS             28697..29293
                     /locus_tag="IVK78_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6373534.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IVK78_000028"
     CDS             29415..30563
                     /locus_tag="IVK78_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6373535.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IVK78_000029"
     CDS             complement(30601..31014)
                     /locus_tag="IVK78_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP6373536.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IVK78_000030"
     CDS             31093..31524
                     /locus_tag="IVK78_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP6373537.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IVK78_000031"
     CDS             complement(31623..31856)
                     /locus_tag="IVK78_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP6373538.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IVK78_000032"
     CDS             32039..33709
                     /locus_tag="IVK78_000032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP6373539.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IVK78_000033"
     CDS             33856..34242
                     /locus_tag="IVK78_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373540.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IVK78_000034"
     CDS             34229..34927
                     /locus_tag="IVK78_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6373541.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IVK78_000035"
     CDS             34932..35396
                     /locus_tag="IVK78_000035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP6373542.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IVK78_000036"
     CDS             35393..36304
                     /locus_tag="IVK78_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6373543.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IVK78_000037"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IVK78_000037"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP6373544.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IVK78_000038"
     CDS             37092..38375
                     /locus_tag="IVK78_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP6373545.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IVK78_000039"
     CDS             38388..39314
                     /locus_tag="IVK78_000039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP6373546.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IVK78_000040"
     CDS             39333..40613
                     /locus_tag="IVK78_000040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP6373547.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IVK78_000041"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IVK78_000041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP6373548.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IVK78_000042"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IVK78_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP6373549.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IVK78_000043"
     CDS             44938..45714
                     /locus_tag="IVK78_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP6373550.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IVK78_000044"
     CDS             45711..46643
                     /locus_tag="IVK78_000044"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP6373551.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IVK78_000045"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IVK78_000045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP6373552.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IVK78_000046"
     CDS             47363..47992
                     /locus_tag="IVK78_000046"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP6373553.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IVK78_000047"
     CDS             47980..48294
                     /locus_tag="IVK78_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373554.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IVK78_000048"
     CDS             48332..48973
                     /locus_tag="IVK78_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6373555.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IVK78_000049"
     CDS             49004..49708
                     /locus_tag="IVK78_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP6373556.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IVK78_000050"
     CDS             49705..50607
                     /locus_tag="IVK78_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6373557.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IVK78_000051"
     CDS             complement(50765..52471)
                     /locus_tag="IVK78_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP6373558.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IVK78_000052"
     CDS             52661..53284
                     /locus_tag="IVK78_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373559.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKALIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IVK78_000053"
     CDS             53734..54783
                     /locus_tag="IVK78_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6373560.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IVK78_000054"
     CDS             54780..55661
                     /locus_tag="IVK78_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6373561.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IVK78_000055"
     CDS             55664..56434
                     /locus_tag="IVK78_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6373562.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IVK78_000056"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IVK78_000056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP6373563.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IVK78_000057"
     CDS             57745..58908
                     /locus_tag="IVK78_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6373564.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IVK78_000058"
     CDS             59025..59435
                     /locus_tag="IVK78_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6373565.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IVK78_000059"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IVK78_000059"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6373566.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGNLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IVK78_000060"
     CDS             61975..62292
                     /locus_tag="IVK78_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373567.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IVK78_000061"
     CDS             62452..62688
                     /locus_tag="IVK78_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6373568.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63083
                     /locus_tag="IVK78_000062"
     CDS             62685..63083
                     /locus_tag="IVK78_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP6373569.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63750..64694)
                     /locus_tag="IVK78_000063"
     CDS             complement(63750..64694)
                     /locus_tag="IVK78_000063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6373570.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64990..65934)
                     /locus_tag="IVK78_000064"
     CDS             complement(64990..65934)
                     /locus_tag="IVK78_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6373571.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66155..67369
                     /locus_tag="IVK78_000065"
     CDS             66155..67369
                     /locus_tag="IVK78_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6373572.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67391..69406
                     /locus_tag="IVK78_000066"
     CDS             67391..69406
                     /locus_tag="IVK78_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP6373573.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69489..70862)
                     /locus_tag="IVK78_000067"
     CDS             complement(69489..70862)
                     /locus_tag="IVK78_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6373574.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70888..71172)
                     /locus_tag="IVK78_000068"
     CDS             complement(70888..71172)
                     /locus_tag="IVK78_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6373575.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71174..73189)
                     /locus_tag="IVK78_000069"
     CDS             complement(71174..73189)
                     /locus_tag="IVK78_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6373576.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73368..73673)
                     /locus_tag="IVK78_000070"
     CDS             complement(73368..73673)
                     /locus_tag="IVK78_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP6373577.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73762..74793)
                     /locus_tag="IVK78_000071"
     CDS             complement(73762..74793)
                     /locus_tag="IVK78_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6373578.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75000..75875
                     /locus_tag="IVK78_000072"
     CDS             75000..75875
                     /locus_tag="IVK78_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6373579.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76027..76869)
                     /locus_tag="IVK78_000073"
     CDS             complement(76027..76869)
                     /locus_tag="IVK78_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6373580.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76961..78838)
                     /locus_tag="IVK78_000074"
     CDS             complement(76961..78838)
                     /locus_tag="IVK78_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6373581.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78835..80256)
                     /locus_tag="IVK78_000075"
     CDS             complement(78835..80256)
                     /locus_tag="IVK78_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6373582.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80259..83648)
                     /locus_tag="IVK78_000076"
     CDS             complement(80259..83648)
                     /locus_tag="IVK78_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6373583.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83905..85297
                     /locus_tag="IVK78_000077"
                     /pseudo
     CDS             83905..85297
                     /locus_tag="IVK78_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(85370..86017)
                     /locus_tag="IVK78_000078"
     CDS             complement(85370..86017)
                     /locus_tag="IVK78_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6373584.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86025..86711)
                     /locus_tag="IVK78_000079"
     CDS             complement(86025..86711)
                     /locus_tag="IVK78_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6373585.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86717..87790)
                     /locus_tag="IVK78_000080"
     CDS             complement(86717..87790)
                     /locus_tag="IVK78_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6373586.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88012..88686
                     /locus_tag="IVK78_000081"
     CDS             88012..88686
                     /locus_tag="IVK78_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6373587.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88691..90031
                     /locus_tag="IVK78_000082"
     CDS             88691..90031
                     /locus_tag="IVK78_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6373588.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90180..91166)
                     /locus_tag="IVK78_000083"
     CDS             complement(90180..91166)
                     /locus_tag="IVK78_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP6373589.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91890..92921
                     /locus_tag="IVK78_000084"
     CDS             91890..92921
                     /locus_tag="IVK78_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6373590.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93030..>93453
                     /locus_tag="IVK78_000085"
     CDS             93030..>93453
                     /locus_tag="IVK78_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6373591.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        29893 a        16576 c        18964 g        28020 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcacaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggcgt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg atttgactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gaaatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca ggcgaaatga
    62761 tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca caagcagtat
    62821 tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca attaatttag
    62881 ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc atgttgttgt
    62941 ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt gattttatca
    63001 taatgattac cactagttca ttggatttcg attcactaaa gtccaagata accagttatt
    63061 tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt agatcagcat
    63121 gtcattaagg aatatgctga tttttttatg tttaatcata caaaatagtt ttttcataaa
    63181 aatatggaaa atatggatta attttgaaat aattgattta aaaggaatac aaaagagtga
    63241 atagtaggaa atagtggttt agaactagtg ggctgatttt ttatatattt tagtttacat
    63301 aatatatatt atacaaagta gatttaaaaa atgataaacc tctctcgaac ggttttttat
    63361 caaaaatcgt catttttcca tcaaaatctg cttatgcaaa attataaaaa aattttgcag
    63421 tttttcttac tttttttgtc gtttaattga atgataaaaa ccagtttctt tattcagtta
    63481 atcaaatatg aacgaattag atatgaatca ttgtatttgc cgttgcggaa ggatttttta
    63541 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
    63601 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tgtttatttg
    63661 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
    63721 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
    63781 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
    63841 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
    63901 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
    63961 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
    64021 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
    64081 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
    64141 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
    64201 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
    64261 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
    64321 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
    64381 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
    64441 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
    64501 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
    64561 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
    64621 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
    64681 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
    64741 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
    64801 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
    64861 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
    64921 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
    64981 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
    65041 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
    65101 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
    65161 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
    65221 aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
    65281 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
    65341 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
    65401 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
    65461 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
    65521 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
    65581 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
    65641 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
    65701 attaataatt tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
    65761 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
    65821 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
    65881 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa
    65941 tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta
    66001 ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata aagactttct
    66061 atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat
    66121 atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa
    66181 attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat
    66241 tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac
    66301 tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat
    66361 cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc
    66421 cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt
    66481 tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg
    66541 agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt
    66601 tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag
    66661 cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca
    66721 atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta
    66781 tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac
    66841 agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat
    66901 gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg
    66961 ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt
    67021 gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt
    67081 cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa
    67141 tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct
    67201 ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat
    67261 cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac
    67321 agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag
    67381 gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt
    67441 tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc
    67501 agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct
    67561 tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg
    67621 acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat
    67681 ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc
    67741 agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct
    67801 ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc
    67861 agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg
    67921 ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga
    67981 gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag
    68041 aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg
    68101 aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc
    68161 aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg
    68221 tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc
    68281 gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac
    68341 gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta
    68401 ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt
    68461 tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa
    68521 tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat
    68581 ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat
    68641 gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca
    68701 tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga
    68761 agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc
    68821 gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa
    68881 gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga
    68941 acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta
    69001 ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca
    69061 aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc
    69121 atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa
    69181 gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg
    69241 tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat
    69301 tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc
    69361 aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct
    69421 tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg
    69481 aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag
    69541 ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg
    69601 tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta
    69661 gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc
    69721 aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg
    69781 aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta
    69841 gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag
    69901 ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta
    69961 gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc
    70021 attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt
    70081 gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct
    70141 aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct
    70201 cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca
    70261 aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca
    70321 acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt
    70381 aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc
    70441 gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga
    70501 aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc
    70561 atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg
    70621 ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt
    70681 gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata
    70741 gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc
    70801 aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc
    70861 attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc
    70921 ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga
    70981 agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac
    71041 acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag
    71101 tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc
    71161 aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg
    71221 atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca
    71281 aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat
    71341 gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt
    71401 cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa
    71461 acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat
    71521 tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat
    71581 ccgataattg gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct
    71641 tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta
    71701 cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca
    71761 ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca
    71821 agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag
    71881 gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg
    71941 aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca
    72001 atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt
    72061 ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt
    72121 cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt
    72181 agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat
    72241 aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa
    72301 acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt
    72361 ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa
    72421 gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt
    72481 gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca
    72541 tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc
    72601 gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt
    72661 tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa
    72721 gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc
    72781 ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt
    72841 catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt
    72901 gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc
    72961 ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc
    73021 agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag
    73081 gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta
    73141 gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca
    73201 cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt
    73261 acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa
    73321 agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca
    73381 tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg
    73441 atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa
    73501 ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct
    73561 tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta
    73621 ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt
    73681 cctcctagta gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca
    73741 aacccgattc ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc
    73801 ttcttccaaa attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc
    73861 attaactaat gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat
    73921 tactttttct tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc
    73981 gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc
    74041 ttgtcgatct tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg
    74101 acgttctttg atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa
    74161 atccagatcg aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct
    74221 gatatcatct ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc
    74281 atataaatag ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa
    74341 ataatcataa tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt
    74401 ttggactgtc aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc
    74461 tttttctttt gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt
    74521 ctctactagt acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg
    74581 actaggtgta gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg
    74641 atcagtatat tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt
    74701 tttgatattg attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact
    74761 ctttccattg ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt
    74821 tcagtatatc ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc
    74881 agaaaaacac ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat
    74941 caaaaagata gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca
    75001 tgcttattga agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg
    75061 tcgattacat ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa
    75121 aagctacgta tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg
    75181 gatttaaaga attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca
    75241 atcaaacgat tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa
    75301 agaaaatggc ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg
    75361 aagatttaga tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa
    75421 aaggtgattc tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt
    75481 ttttgatcat ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg
    75541 aggattgcgc catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga
    75601 cctatttgaa gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa
    75661 tggccaaaca ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag
    75721 tagcgacttt ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga
    75781 tatattctca aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc
    75841 aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag
    75901 aaagatagtg tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt
    75961 tttcaaacct cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag
    76021 gaatagctaa cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag
    76081 gaaaatcagt atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac
    76141 tttctgtact tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag
    76201 taagcgatga ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc
    76261 tttttcgatt ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg
    76321 ataagcgagt gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg
    76381 taagtctgtg aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt
    76441 attttctccc cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag
    76501 cacagtattc gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat
    76561 accgatcgta tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt
    76621 aaagggatct gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat
    76681 gttttcttgg attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg
    76741 agagatgatc tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag
    76801 tatccctatt cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt
    76861 tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt
    76921 gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc
    76981 gtctagcaaa gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat
    77041 agattccctt accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag
    77101 gagaaatcaa ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt
    77161 ttgttaatgg aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt
    77221 ttagacctgt cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt
    77281 tcgccgatac ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt
    77341 cactatcttg atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt
    77401 caccatctac tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt
    77461 gatcatttgt atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg
    77521 ctttttcgtt caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag
    77581 gaatataatt cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat
    77641 ttactgggtc gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat
    77701 cattatcaaa tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt
    77761 tttgcgttcc ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa
    77821 ttttttcacc gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag
    77881 acgtatcatt tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag
    77941 gcgtgattgt ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat
    78001 tatattttcc aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg
    78061 caaagtcatt gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt
    78121 gatcagttgt ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg
    78181 gtgttccttc ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag
    78241 taccgatttt tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat
    78301 ggatagtatc tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct
    78361 catatacagg gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt
    78421 ctttgatcgt gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat
    78481 ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg
    78541 cttgttttgc tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga
    78601 cgttataaac tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt
    78661 ctttcccgct gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga
    78721 tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta
    78781 atggtaaaat aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt
    78841 tttcactcct gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc
    78901 aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt
    78961 ttctttttta ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt
    79021 tgaaatggta aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc
    79081 ttttaatcga taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga
    79141 aatcaggtga agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg
    79201 gaggtaattt cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg
    79261 tttatttgtc tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt
    79321 actgacacca aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc
    79381 tgtactgctt agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa
    79441 cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc
    79501 tgtcagcttt tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc
    79561 atccgcacta tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt
    79621 atgcaaggaa taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac
    79681 cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt
    79741 tgaaagtttt tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt
    79801 ttctttgacg acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc
    79861 ttgatcttta gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt
    79921 tgttgcttct gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc
    79981 ttcttctacc gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac
    80041 ttggaatgtg acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc
    80101 atttggatga tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg
    80161 aaccatttct gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa
    80221 gaagagacac atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt
    80281 ttcaaaaaaa gataccaaat agaaaacgca caaccaatca tccctactag atagattcct
    80341 aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa
    80401 gaaatctggt tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact
    80461 tgaattgacc catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct
    80521 tctggtgtaa aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag
    80581 gtatctgttt cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg
    80641 aattttgctc cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac
    80701 gcttttagtc gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt
    80761 cccgtgtaaa tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta
    80821 tcatatccgg caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca
    80881 aaactggcaa gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt
    80941 gcttcttttt tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct
    81001 tgcatcgcat acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac
    81061 tggatcgtat catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa
    81121 gcgatcttga tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt
    81181 tctgtcagcg tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt
    81241 ccgtcgccat gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg
    81301 ccaataagat ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg
    81361 aattgtttag tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca
    81421 ggtaattctt tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt
    81481 gataatatct cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt
    81541 tcccacgtat ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa
    81601 gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca
    81661 gctagattgt tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct
    81721 ttagcagatg gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt
    81781 gtcctgccgt tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt
    81841 ctcacttggt aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg
    81901 atggtatttc cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc
    81961 ttgactgaaa gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt
    82021 ccattgctta cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct
    82081 gttgcatgat cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt
    82141 cgcatacgag actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg
    82201 atgccaagac catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt
    82261 gcgataaacg tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt
    82321 cttgagatta gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa
    82381 taattgctgc ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca
    82441 tccgtcagca aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca
    82501 tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt
    82561 acttgatttt tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat
    82621 ccttcgcttg agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct
    82681 aaagtatcga caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc
    82741 atacttcctg accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca
    82801 tttccacgag cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac
    82861 tttcgtatcg catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc
    82921 cgatcattag gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta
    82981 ttgccttgat gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa
    83041 gtgccctgat catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca
    83101 gctggcaagg ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat
    83161 gattcttctt ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct
    83221 gtctcatcaa taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg
    83281 taaagtgctg gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt
    83341 tccaacgtaa ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc
    83401 acatcactta agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg
    83461 ttattcgcat ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca
    83521 gctgaaattg tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc
    83581 acaaagtttg ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg
    83641 gtcgtcattt ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat
    83701 taaaataaac aggcgttaca taattttatt tattttaata tgataattat tatacgaagg
    83761 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
    83821 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
    83881 ttgttggaac gaaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
    83941 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
    84001 aggtataatc gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca
    84061 aaaattgggg tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga
    84121 aatgtcgcaa agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa
    84181 aacgattgag tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt
    84241 gaaacaatgc aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt
    84301 tcagttaact agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt
    84361 aagttactac ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat
    84421 tatggtcaag aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc
    84481 tttctttatt atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga
    84541 atggaaagct ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat
    84601 cttgaaaaat ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt
    84661 tgtcaatacg attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat
    84721 acgaaaaaat atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga
    84781 atatgttgtt caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac
    84841 gactcatttg acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg
    84901 tctatatgct ggatttcgtg cagaacttca attttattcc actctactga atcagaatct
    84961 gatttattat ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca
    85021 ttatacttct gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata
    85081 tattgaagaa tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc
    85141 tattactgaa gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa
    85201 tcaacccatt ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc
    85261 aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat
    85321 aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc
    85381 cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat
    85441 tatttgcaat gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac
    85501 taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt
    85561 tttcgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc
    85621 gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc
    85681 actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta
    85741 agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca
    85801 aatcttgaaa atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg
    85861 agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc
    85921 tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt
    85981 tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc
    86041 tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata
    86101 cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt
    86161 aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag
    86221 atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca
    86281 gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa
    86341 tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc
    86401 tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag
    86461 cgtaaatctg ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt
    86521 tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa
    86581 ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc
    86641 aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat
    86701 atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac
    86761 gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc
    86821 attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag
    86881 caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag
    86941 atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct
    87001 tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa
    87061 agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga
    87121 atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg
    87181 aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc
    87241 catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat
    87301 aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg
    87361 tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg
    87421 ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga
    87481 aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg
    87541 ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata
    87601 cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt
    87661 taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca
    87721 agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag
    87781 ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg
    87841 aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat
    87901 tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt
    87961 atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa
    88021 ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca
    88081 atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga
    88141 gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta
    88201 gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt
    88261 ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc
    88321 tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt
    88381 gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg
    88441 tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta
    88501 caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat
    88561 tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga
    88621 agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg
    88681 gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa
    88741 tgtagcagca tttgctttta tcttttttct tttgggattc attactttac agatattaaa
    88801 ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac
    88861 agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac
    88921 ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa
    88981 cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg
    89041 gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa
    89101 tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa
    89161 agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat
    89221 caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat
    89281 tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc
    89341 ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac
    89401 gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga
    89461 atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga
    89521 tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa
    89581 tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg
    89641 aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat
    89701 ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa
    89761 gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg
    89821 cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca
    89881 atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat
    89941 cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa
    90001 agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa
    90061 aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg
    90121 gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct
    90181 tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct
    90241 tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca
    90301 acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata
    90361 gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt
    90421 ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg
    90481 accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat
    90541 ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca
    90601 gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt
    90661 gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga
    90721 tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct
    90781 tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca
    90841 aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc
    90901 gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg
    90961 tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga caatccgtca
    91021 aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac
    91081 ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg
    91141 atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg
    91201 tatattgtta cttataaatt tattctatag gagacgattg ttttctacaa atatttagat
    91261 tggaatttga ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat
    91321 attctaataa aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc
    91381 cactcgttgt gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa
    91441 aggattaatt ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga
    91501 agtaccaact atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc
    91561 attattgaat aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa
    91621 taaaagaaag agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta
    91681 accacatagc taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata
    91741 aaataactaa aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt
    91801 tgcttcctaa gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag
    91861 aaaatgaaca ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag
    91921 aaaagttaaa gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa
    91981 atcgttcttc cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta
    92041 ctgattattt gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag
    92101 aaaaacaaga ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa
    92161 ctacagcgaa ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga
    92221 agcaatattc attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag
    92281 agatttctct tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg
    92341 atgcagaact tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa
    92401 gcagagaagc tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc
    92461 ttctgtattg cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt
    92521 tgaccaagca gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac
    92581 caaaagaagc gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg
    92641 aaaattcttt tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc
    92701 aaattttgag caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat
    92761 atcgttatta tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta
    92821 atcgatacct ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt
    92881 atcacgaatt agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt
    92941 tttcacaata aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat
    93001 attacttaac aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccctttga
    93061 acttatttac gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg
    93121 atgagccact ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg
    93181 aaagtgtgtt gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag
    93241 ttattattta taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc
    93301 ttggtgcagt gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta
    93361 tcgatcgttt agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag
    93421 aaatttcaaa tggaacaaaa tcaaaacaga ttc
//
LOCUS       DACCCM010000002        85502 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCCM010000002 DACCCM010000000
VERSION     DACCCM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.002"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            170..1228
                     /locus_tag="IVK78_000086"
     CDS             170..1228
                     /locus_tag="IVK78_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP6373592.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1247..2266
                     /locus_tag="IVK78_000087"
     CDS             1247..2266
                     /locus_tag="IVK78_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP6373593.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2368..3084)
                     /locus_tag="IVK78_000088"
     CDS             complement(2368..3084)
                     /locus_tag="IVK78_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6373594.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3053..3838)
                     /locus_tag="IVK78_000089"
     CDS             complement(3053..3838)
                     /locus_tag="IVK78_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6373595.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3829..4578)
                     /locus_tag="IVK78_000090"
     CDS             complement(3829..4578)
                     /locus_tag="IVK78_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP6373596.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4876..5400
                     /locus_tag="IVK78_000091"
     CDS             4876..5400
                     /locus_tag="IVK78_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373597.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5416..6915
                     /locus_tag="IVK78_000092"
     CDS             5416..6915
                     /locus_tag="IVK78_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP6373598.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7003..7605)
                     /locus_tag="IVK78_000093"
     CDS             complement(7003..7605)
                     /locus_tag="IVK78_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6373599.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7709..8665
                     /locus_tag="IVK78_000094"
     CDS             7709..8665
                     /locus_tag="IVK78_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP6373600.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8970..9926)
                     /locus_tag="IVK78_000095"
     CDS             complement(8970..9926)
                     /locus_tag="IVK78_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6373601.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10100..10660
                     /locus_tag="IVK78_000096"
     CDS             10100..10660
                     /locus_tag="IVK78_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373602.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10653..11477
                     /locus_tag="IVK78_000097"
     CDS             10653..11477
                     /locus_tag="IVK78_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP6373603.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11487..12104
                     /locus_tag="IVK78_000098"
     CDS             11487..12104
                     /locus_tag="IVK78_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP6373604.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12122..12805
                     /locus_tag="IVK78_000099"
     CDS             12122..12805
                     /locus_tag="IVK78_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6373605.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13488..14771
                     /locus_tag="IVK78_000100"
     CDS             13488..14771
                     /locus_tag="IVK78_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6373606.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14787..15674
                     /locus_tag="IVK78_000101"
     CDS             14787..15674
                     /locus_tag="IVK78_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6373607.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15691..16527
                     /locus_tag="IVK78_000102"
     CDS             15691..16527
                     /locus_tag="IVK78_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6373608.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16530..17153
                     /locus_tag="IVK78_000103"
     CDS             16530..17153
                     /locus_tag="IVK78_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373609.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17150..18442
                     /locus_tag="IVK78_000104"
     CDS             17150..18442
                     /locus_tag="IVK78_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6373610.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18801..18898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19014..19595
                     /locus_tag="IVK78_000105"
     CDS             19014..19595
                     /locus_tag="IVK78_000105"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP6373611.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19598..20905
                     /locus_tag="IVK78_000106"
     CDS             19598..20905
                     /locus_tag="IVK78_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP6373612.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21321..21809
                     /gene="purE"
                     /locus_tag="IVK78_000107"
     CDS             21321..21809
                     /gene="purE"
                     /locus_tag="IVK78_000107"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP6373613.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21793..22926
                     /gene="purK"
                     /locus_tag="IVK78_000108"
     CDS             21793..22926
                     /gene="purK"
                     /locus_tag="IVK78_000108"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP6373614.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22939..24234
                     /gene="purB"
                     /locus_tag="IVK78_000109"
     CDS             22939..24234
                     /gene="purB"
                     /locus_tag="IVK78_000109"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP6373615.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24414..24791)
                     /locus_tag="IVK78_000110"
     CDS             complement(24414..24791)
                     /locus_tag="IVK78_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373616.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            25047..25775
                     /locus_tag="IVK78_000111"
     CDS             25047..25775
                     /locus_tag="IVK78_000111"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP6373617.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25775..26029
                     /gene="purS"
                     /locus_tag="IVK78_000112"
     CDS             25775..26029
                     /gene="purS"
                     /locus_tag="IVK78_000112"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP6373618.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            26031..26702
                     /gene="purQ"
                     /locus_tag="IVK78_000113"
     CDS             26031..26702
                     /gene="purQ"
                     /locus_tag="IVK78_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP6373619.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26703..28925
                     /gene="purL"
                     /locus_tag="IVK78_000114"
     CDS             26703..28925
                     /gene="purL"
                     /locus_tag="IVK78_000114"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP6373620.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28910..30349
                     /locus_tag="IVK78_000115"
     CDS             28910..30349
                     /locus_tag="IVK78_000115"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP6373621.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30381..31424
                     /locus_tag="IVK78_000116"
     CDS             30381..31424
                     /locus_tag="IVK78_000116"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP6373622.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            31421..31999
                     /gene="purN"
                     /locus_tag="IVK78_000117"
     CDS             31421..31999
                     /gene="purN"
                     /locus_tag="IVK78_000117"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP6373623.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31996..33531
                     /gene="purH"
                     /locus_tag="IVK78_000118"
     CDS             31996..33531
                     /gene="purH"
                     /locus_tag="IVK78_000118"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP6373624.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33547..34821
                     /gene="purD"
                     /locus_tag="IVK78_000119"
     CDS             33547..34821
                     /gene="purD"
                     /locus_tag="IVK78_000119"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP6373625.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34943..35857
                     /locus_tag="IVK78_000120"
     CDS             34943..35857
                     /locus_tag="IVK78_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP6373626.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35990..37555)
                     /locus_tag="IVK78_000121"
     CDS             complement(35990..37555)
                     /locus_tag="IVK78_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6373627.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37548..38999)
                     /locus_tag="IVK78_000122"
     CDS             complement(37548..38999)
                     /locus_tag="IVK78_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6373628.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(39013..40029)
                     /locus_tag="IVK78_000123"
     CDS             complement(39013..40029)
                     /locus_tag="IVK78_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6373629.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40249..41337
                     /locus_tag="IVK78_000124"
     CDS             40249..41337
                     /locus_tag="IVK78_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6373630.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41391..42917
                     /locus_tag="IVK78_000125"
     CDS             41391..42917
                     /locus_tag="IVK78_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP6373631.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42919..44124
                     /locus_tag="IVK78_000126"
     CDS             42919..44124
                     /locus_tag="IVK78_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6373632.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44381..45358
                     /gene="guaC"
                     /locus_tag="IVK78_000127"
     CDS             44381..45358
                     /gene="guaC"
                     /locus_tag="IVK78_000127"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP6373633.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45622..47046
                     /locus_tag="IVK78_000128"
     CDS             45622..47046
                     /locus_tag="IVK78_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6373634.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47507..48616
                     /gene="pdhA"
                     /locus_tag="IVK78_000129"
     CDS             47507..48616
                     /gene="pdhA"
                     /locus_tag="IVK78_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP6373635.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48619..49596
                     /locus_tag="IVK78_000130"
     CDS             48619..49596
                     /locus_tag="IVK78_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP6373636.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49621..51264
                     /locus_tag="IVK78_000131"
     CDS             49621..51264
                     /locus_tag="IVK78_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP6373637.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51272..52678
                     /gene="lpdA"
                     /locus_tag="IVK78_000132"
     CDS             51272..52678
                     /gene="lpdA"
                     /locus_tag="IVK78_000132"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP6373638.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52840..53115
                     /locus_tag="IVK78_000133"
     CDS             52840..53115
                     /locus_tag="IVK78_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP6373639.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            53121..53888
                     /locus_tag="IVK78_000134"
                     /pseudo
     CDS             53121..53888
                     /locus_tag="IVK78_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
     gene            54056..55885
                     /gene="typA"
                     /locus_tag="IVK78_000135"
     CDS             54056..55885
                     /gene="typA"
                     /locus_tag="IVK78_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP6373640.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            56146..57918
                     /gene="recQ"
                     /locus_tag="IVK78_000136"
     CDS             56146..57918
                     /gene="recQ"
                     /locus_tag="IVK78_000136"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP6373641.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            58032..58334
                     /locus_tag="IVK78_000137"
     CDS             58032..58334
                     /locus_tag="IVK78_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP6373642.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58337..59500
                     /locus_tag="IVK78_000138"
     CDS             58337..59500
                     /locus_tag="IVK78_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6373643.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59526..62954
                     /locus_tag="IVK78_000139"
     CDS             59526..62954
                     /locus_tag="IVK78_000139"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP6373644.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62975..64099
                     /locus_tag="IVK78_000140"
     CDS             62975..64099
                     /locus_tag="IVK78_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373645.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            64089..64526
                     /locus_tag="IVK78_000141"
     CDS             64089..64526
                     /locus_tag="IVK78_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6373646.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64510..64848
                     /locus_tag="IVK78_000142"
     CDS             64510..64848
                     /locus_tag="IVK78_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP6373647.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64967..65527
                     /gene="rsmD"
                     /locus_tag="IVK78_000143"
     CDS             64967..65527
                     /gene="rsmD"
                     /locus_tag="IVK78_000143"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP6373648.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65524..66015
                     /gene="coaD"
                     /locus_tag="IVK78_000144"
     CDS             65524..66015
                     /gene="coaD"
                     /locus_tag="IVK78_000144"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP6373649.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            66005..67048
                     /locus_tag="IVK78_000145"
     CDS             66005..67048
                     /locus_tag="IVK78_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6373650.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            67207..67935
                     /locus_tag="IVK78_000146"
     CDS             67207..67935
                     /locus_tag="IVK78_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP6373651.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            68051..68296
                     /locus_tag="IVK78_000147"
     CDS             68051..68296
                     /locus_tag="IVK78_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6373652.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68293..68511
                     /locus_tag="IVK78_000148"
     CDS             68293..68511
                     /locus_tag="IVK78_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP6373653.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68647..69186
                     /locus_tag="IVK78_000149"
     CDS             68647..69186
                     /locus_tag="IVK78_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP6373654.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            69179..71452
                     /locus_tag="IVK78_000150"
     CDS             69179..71452
                     /locus_tag="IVK78_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP6373655.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71490..72536
                     /gene="holA"
                     /locus_tag="IVK78_000151"
     CDS             71490..72536
                     /gene="holA"
                     /locus_tag="IVK78_000151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP6373656.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72625..72879)
                     /locus_tag="IVK78_000152"
     CDS             complement(72625..72879)
                     /locus_tag="IVK78_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP6373657.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(73279..73548)
                     /locus_tag="IVK78_000153"
     CDS             complement(73279..73548)
                     /locus_tag="IVK78_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373658.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(74018..74473)
                     /locus_tag="IVK78_000154"
     CDS             complement(74018..74473)
                     /locus_tag="IVK78_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP6373659.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74611..76540)
                     /locus_tag="IVK78_000155"
                     /pseudo
     CDS             complement(74611..76540)
                     /locus_tag="IVK78_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(76737..77570)
                     /locus_tag="IVK78_000156"
     CDS             complement(76737..77570)
                     /locus_tag="IVK78_000156"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP6373660.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(77762..78466)
                     /locus_tag="IVK78_000157"
     CDS             complement(77762..78466)
                     /locus_tag="IVK78_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6373661.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(78489..79115)
                     /locus_tag="IVK78_000158"
     CDS             complement(78489..79115)
                     /locus_tag="IVK78_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6373662.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            79441..81816
                     /locus_tag="IVK78_000159"
     CDS             79441..81816
                     /locus_tag="IVK78_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP6373663.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(81840..82331)
                     /locus_tag="IVK78_000160"
     CDS             complement(81840..82331)
                     /locus_tag="IVK78_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP6373664.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            82518..83438
                     /locus_tag="IVK78_000161"
     CDS             82518..83438
                     /locus_tag="IVK78_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6373665.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            83592..83936
                     /locus_tag="IVK78_000162"
     CDS             83592..83936
                     /locus_tag="IVK78_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP6373666.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(84126..84626)
                     /locus_tag="IVK78_000163"
     CDS             complement(84126..84626)
                     /locus_tag="IVK78_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6373667.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            85154..85237
                     /locus_tag="IVK78_000164"
     tRNA            85154..85237
                     /locus_tag="IVK78_000164"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:85188..85190,aa:Leu,seq:cag)
BASE COUNT        27764 a        14785 c        18327 g        24626 t
ORIGIN      
        1 tttttttgtg ttctagtaaa aatcacttta cataagtttc aaaagtttct ttaatttcct
       61 attgttcaat aagtaacaat gtaaagataa aattaaaaat gttaatacgt attagcaaaa
      121 taaaagcttt ttttattgtg aataaatgaa cataattagg aggagaaaga tgaacttatt
      181 tgacattttt cgatcgactt tgacagatat gtcgattgtc agtgcaatca cttcaacagt
      241 ttttattata ttattagggt ttgtttgtag aaaaaagggg attttttctg aatctgttgg
      301 aaaaattcta tcgaaagtcg tcttaacagt ggctttgcca gcactggcat ttcgagcttt
      361 catgcaagat atcaatacag aattactgat gcaaggaatc aatgtcttga tttggggatt
      421 agttatttat attattctta ttctagtttc aaaacctttt ttcttaagct ataaagggga
      481 taagcaaaca acgttgcgtg tcctgacgat ctttggttct acgacctttt tcggaacgcc
      541 tatcgtcagt gcgatttatg gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg
      601 ctaccgaatc tttttgtatt cttatggata cataaaaatg agtggcttga agatggaagc
      661 taaaaatatc aaagcgatgt tcgtcaatcc aatcattatc gctacttttg ccggcctttt
      721 catttggctg ttccaagggt atctgccaca agtgtcagtt acaggtgcag aaggagagat
      781 aagccaagta gcgtttttgc gaattgacca gactgcaccg tggctatttc agccgatgga
      841 ttatttagct ggtttaagtt caccattggc gtggctagca atcggcgcaa cattaggtga
      901 agtaagcttc aaaaaagctg catctgaaaa aacttcatgg tattacagtc tagtcaaagt
      961 ggtatttgta cctgcattga atatcgtgat tctagcagtt ttgacggcaa cgaatattct
     1021 accagtcaat tttgaagcac tggcaacaat cgttatcatg atggctacac cagctgcaac
     1081 agtggcagca gcctatgcta tcagttttga tagggaggca cttttagcct ccaatgcatc
     1141 cttactgtca actatttttg ctgtggtact gacacctgta tggatcgttg tattacaaat
     1201 gattcatggc ataggtttat tttcataaga aatggaggaa gaaagtatga gaatcgcatg
     1261 ttacggtgtt cgtccgaacg agatagattt ttttgaaaaa ttgaatattt accattatga
     1321 tctcagtctt tatgaagaac tgttgacaca tgacaatatc gtgtcagcaa aggaccatga
     1381 tgctgtattg ttgaggggaa attgtatagc agatgagacc aatcttgcaa aaatgcagga
     1441 atatgggatt cgttatgttt ttacacggac tgtgggagtc aatcatatcg atctacaagc
     1501 ggcagctgat tttggtatga cagtagcccg tgtaccatcg tattcgccaa atgcgatagc
     1561 agagttatct ttgacttttg ccatgatgct tttgagaaat actgcgtata cgacgatccg
     1621 aacatctttc aaagattttc gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg
     1681 cacagtaggg atcataggaa caggcaggat agggttgacg gaagctgttt tgtttaaggg
     1741 actaggagcc aaagtgatcg gctacgatat tcatcagaca gaagctgcta aaaaagtatt
     1801 gacgttccac tcgcttgaag agttattagc caaaagcgat attgtcagta ttcatgtacc
     1861 tcatatccca ggtgaaaacg atcaaatgat caatgaagga tttttagctc aaatgaaaaa
     1921 aggtagcatc ttgattaata cagcccgtgg agagcttcaa gataatgaag ctattttaag
     1981 agctttgaaa agcaatcatt tagctggttt tgcaaccgat gtttttgcaa atgaaacaga
     2041 ggtttttttc cgatccttca agccatggga gacgattcca gaccctgcta tccagcaatt
     2101 agtagaaatg taccctagag ttcttgtaac cccgcatgta ggttcaaata cagatgaggc
     2161 attatcaaat atgatttcga caagtttcga gaactttcgt gaaataatag aaactggaaa
     2221 aacgaaaaat gaggtatctc ttcctaaagc ccgtcaatta agataagaaa acatggatat
     2281 tcgtcaataa aatgtttaaa aatagtccaa gtaaacagtt gttacttctg tatacttgga
     2341 ctattttgta tgggataatt cgatgactca acagtaatct gtgagctttt tccgcccatt
     2401 ctttgtcaat tcataacagg ttagcggccg gccgatttcc aaatatttga ttctttcgtt
     2461 caatatatct tgttctacta aaaaattcaa atatttcttg acggatatac gtgacagtga
     2521 gcttttttga gccagttctt ttgttgaaaa aatagtatct tcttttagaa taaatgcact
     2581 tatttttttt aaagtcagtt ttgacagacc tttaggcaaa tcgatgaatg atggtatttc
     2641 gtgaatggga gtggtgtggt tgaatacttg atcgatcaat ttctgatcgg ctttttcagt
     2701 cgtttggatt gtctgagaga aagcaagata cttttcgatc gccagctgaa aacgttgcaa
     2761 agtgaacggt ttgatcaaat aatctaaggc accgtagcgg atcacattct ttatggtttc
     2821 gatatcatct gcagcagtga tcaaaatcac tgggatatca tttccttgct gtctaagcca
     2881 agataaaaaa tggataccac tttcgccagg catgtataca tcaagcagca gcagatctgc
     2941 gtttttttgt ctgaaatatt tttttccttc ttctacattt ctgaaacttt cgattacgtt
     3001 catattaggg atgctttcca caaaagtacg attcaattct gcaaccattg gatcatcttc
     3061 gattatcagg acatttgtca tcattttgat cttccttttt tggtaattct agatagacga
     3121 ttgtaccgcc atttgtttgt ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg
     3181 aatggatgag attcaatcca aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa
     3241 atattttttc tgagatttct tctgatatcc cgacaccgtc atcgcagacg gtcacgatga
     3301 atacttcagc ttctgtgtca tagtttaaat gaatccggac ctgtccgttt ctttcatgtt
     3361 tttgtagcac ggcttctttt gcattatcaa gtaagttccc taacacttgc aaaagtgaat
     3421 ggatggagtc atccatctcc attttaggga taaaagaatc gctttctaac agcatttcaa
     3481 cagattgttc tttggcttcg tttattttgc ctaataaaaa accggcaatt gcaggtactt
     3541 tgatcagact tgagacttgg ccaatctctt ttttataatt ttgatttagc tgctggataa
     3601 aatgggtgac ttcttcatac ttcttcaaat caatcaaacc agaaatgaca tgaagtttat
     3661 tcatgaattc atgggtttga gctctcatgg agtttatata ttgtttcgtc cccccaagct
     3721 cctccattaa tttcattact tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac
     3781 ggtcattgct aaaaagaggt gtaacgctag aaatagtttc tatcccattt aaatacatgg
     3841 attgagtccg aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat
     3901 tatcactgat ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt
     3961 ttttgcaggt gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc
     4021 atctacttca ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc
     4081 aagtccaagc agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac
     4141 gctaagtcca ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc
     4201 aatcgtgttg tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat
     4261 agggacgaaa tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg
     4321 tcctccaatc acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg
     4381 gtctgcgtga gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga
     4441 tgcaatcatg atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag
     4501 ctttttttgg atgagtttat cattggcaac gattttagca atgctagaga ttttatctga
     4561 cacactagat cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa
     4621 agaaacgaat gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt
     4681 tttcttaaaa aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat
     4741 gaaaacaaaa atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac
     4801 tgagtcggta aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg
     4861 gaaggaggcg tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg
     4921 ctgatttgtg caggattggc agtcctattg attatttttg gagatgtatt cgattttgat
     4981 ggcccgcttg atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt
     5041 ctaggtgagc gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata
     5101 tcgatagtgt tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa
     5161 tcaacgatgt cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg
     5221 atccctgtac gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt
     5281 ccagcttgtt tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc
     5341 attgagatca aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa
     5401 aggagcgtgt gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg
     5461 attttactga tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa
     5521 gcgttgatca tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat
     5581 aacaaaatca aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg
     5641 aatcggatca gtctattatc gagtaaattg gacgtctcca cccctgaagt gtatacggaa
     5701 caaggcgtac cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa
     5761 gagattgcta cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa
     5821 gcaagagaag tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa
     5881 atctatcaaa atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt
     5941 gcaaaaatgg ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt
     6001 tatcttgact ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca
     6061 gaagcggaag ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa
     6121 gcagctgaat tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa
     6181 ttagcaacct acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat
     6241 ttagaaagtg cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta
     6301 gaacgtcaaa aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat
     6361 gattcggaag tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa
     6421 gctcaaaaag tcaaagaagt tactgaagcc gaagcagaac gattccgtgt ggaagcactg
     6481 gcagaagcgg aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta
     6541 gcaaaaggaa aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat
     6601 ggcgaagcag ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca
     6661 gcacagccgc taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga
     6721 gaaaccagtg gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa
     6781 gaaacactaa aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca
     6841 cgtgggacta ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg
     6901 atgaaggaag aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt
     6961 aaagttcttc tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt
     7021 cttttgtttt tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt
     7081 cttttggagt tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg
     7141 aagaaagaaa ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg
     7201 gatagcatac aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc
     7261 tattgcggaa atgataccgg aattattgtt aataccatct gacacgacga tcacaataat
     7321 aactagaata ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac
     7381 aaaaaaataa atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc
     7441 aaaagaaacg gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat
     7501 catcgggatt cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc
     7561 acttttgatc gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt
     7621 tggtcttttt actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt
     7681 ataattaaaa taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt
     7741 gatcagtgtc ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc
     7801 tgcagggatc attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg
     7861 catgaatcga atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc
     7921 tgttgggtat actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc
     7981 tcgcttcaaa ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt
     8041 ggaggaaaac atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt
     8101 agtatttgct ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa
     8161 ccaaatacag atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt
     8221 atcagaaaat aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga
     8281 aagccaagaa gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta
     8341 cgaaacttac ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa
     8401 tctagagaga atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta
     8461 tactgcagaa acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga
     8521 agtttatgta ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg
     8581 agcagaattg ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt
     8641 ctcccaacaa aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag
     8701 ctgatttcac aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca
     8761 caatattcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga
     8821 acggtaggct cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa
     8881 gtccagccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
     8941 cagaacagct ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga
     9001 tccaagattt cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca
     9061 atcatgtgta ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg
     9121 atgaattcct ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca
     9181 gcaatcgtca cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga
     9241 aaagtcaagc tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt
     9301 tcgtccacgc ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc
     9361 aaaaagaaac gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct
     9421 aattctggat tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct
     9481 tggatcattt ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa
     9541 agtggcatgt tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag
     9601 cgcatagcgt cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt
     9661 ttgcctgctt ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg
     9721 tacacgatgt cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga
     9781 atattgtaac ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt
     9841 agttcgctcg tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca
     9901 acgattcccc attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc
     9961 ttgtctgtct attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat
    10021 taaaaaaaat gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag
    10081 ttaaaaggag gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt
    10141 agaacagttt gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca
    10201 atggcaaaaa tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa
    10261 aaatcttttc actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat
    10321 ttttcccgaa aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc
    10381 gcaaaaatgg cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca
    10441 gcaggctggt agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga
    10501 tgatattttg aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat
    10561 cgaacttctc gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg
    10621 ggaacgtgaa gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag
    10681 ttgtgattat gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc
    10741 aagccggcaa tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga
    10801 acagtcaaac aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac
    10861 aattgccaga tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat
    10921 tagaaaaaga aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg
    10981 acaaacgcat tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc
    11041 cattagtgat ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa
    11101 atgtccaaga tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta
    11161 aagaaacgat tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg
    11221 tcgatcaaaa ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc
    11281 agcctggagc taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc
    11341 caaaaggaca cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg
    11401 gtaaagaaaa cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg
    11461 aattaaatga aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca
    11521 agaatatccg ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt
    11581 tttccttgaa gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat
    11641 cccatcagca atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg
    11701 gagtgcttcc tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta
    11761 ccttggctag caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag
    11821 taagcctgga aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca
    11881 aagtgtatga ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag
    11941 tagattggaa tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg
    12001 cacaagcatt gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc
    12061 aggtcatcga gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct
    12121 gatggcagaa caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt
    12181 accggaaatc attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat
    12241 catctttact acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt
    12301 gtatcctttt acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc
    12361 agttttctcc ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat
    12421 gttcgcagaa gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac
    12481 agtaaaagaa gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata
    12541 tacagaaata gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa
    12601 agaaactgca gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc
    12661 gacaggtggt ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat
    12721 cacatatacg ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga
    12781 aactgagaaa aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag
    12841 tggctcttcc tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa
    12901 atttttttgt ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt
    12961 agcattctaa caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga
    13021 ataaaaacta aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa
    13081 gtcagcggga actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat
    13141 tgtacctaat ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca
    13201 cgaagaagca tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt
    13261 ttctttgcgt aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag
    13321 aatcagcact atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata
    13381 aggcaagtct tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata
    13441 aaaatacagt atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa
    13501 tgagaatcat cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag
    13561 atgacaaaaa aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa
    13621 cagtaaaagt aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat
    13681 tttatgatta tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct
    13741 gggacatttt ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag
    13801 atgaatatgt ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa
    13861 cgacgactta cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg
    13921 cttacaataa aaatatattt gaacaattaa atatccctga accagcttca aattggacat
    13981 gggatgaatt tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt
    14041 caagattcga agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat
    14101 tgaattttta taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc
    14161 taacagattt tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag
    14221 gtggtggagc tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg
    14281 caatggtgtt tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata
    14341 tggagattca gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt
    14401 tacgttcttc gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta
    14461 agtttattga tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg
    14521 gtgtaccaat catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc
    14581 aagcgacatt tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct
    14641 ttgatagtgc tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca
    14701 tttatgaaga agtaacagca gaagaagcag cagagagtct ttatgagttt gcgtcaaaca
    14761 ttctaggata gcagcaggag gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc
    14821 aagacaatgt ggtcggttac gtatttgctt ctccattcat cctaggattt ctattgataa
    14881 cagtgatacc gatgatcttg tcactttatt attcgttcac cgattatcgt ttgggaaatc
    14941 cagtctcatg gatcggcttg gataattata taaagatgct gagtgatgcc cgttttttga
    15001 actccatccg agtcactttt ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg
    15061 gtctattgat tgcttatctt ttaacgagga atatcaaagg aacgactttc tttcgttctt
    15121 tgtattatgt gcctagtttg atcggtggca gtatttctat cgctctagtt tggcgggagt
    15181 tgttttctaa aaacgggtta gtgaatattt tcctggaacg attaggcgca cctgcgcttt
    15241 cctggtttag cgatcaaaaa ctggtcatga tccctcttgt tttgatgtct gcttggcaat
    15301 tcggttcggc aatgatcatc tttgctgcag gtatcaaaga gatcccaggc tcgtattatg
    15361 aagcggcaga gatcgatgga gcgaacaaat ggcagacgtt ttacaagatc acgttacctc
    15421 ttctttcgcc gatcattttg tataacttgg ttatgcagac aatcgcggcg tttatgactt
    15481 ttacgcaggc atttatcatt acccaaggtg gtccgaatga tgccacgaac ttttatgctc
    15541 tttatgtgta taaccaagct ttttctttta attctatggg gtatgctagt gctatttcgt
    15601 ggatcatgct gttgattatg ggagctgtca cctttctgat tttcaaatca tcaaaaaaat
    15661 gggtcgttgt gtgaatgaga ggaggaagaa atgaataata cagtaacgaa atggtttcaa
    15721 tacggtttta ttttgctatt aggtctagtc atgatttatc cggtcttatg gatgatcgca
    15781 ggttctttta aaacggatca agagattatg agtggttcgc tgaatttgat tccagagact
    15841 tttcaatggg aaaattacgc aaaaggatgg gcggggtttg ctggaatttc attttttact
    15901 ttctttagga attcgtttgt catctctgca gtttcgacga tagggacagt tctgagctcg
    15961 acttgtattg cttacgcatt atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc
    16021 gtcatgttag taacgatgat gattcctgca caagtgattt tgattccgca gtttattatt
    16081 tacaatcggc tgaatttggt tgggacgtat gtgccgctga ttttaccaca tttttttgga
    16141 caagcatttt tcatttacca gatcatgcaa ttcatggtca gcatccccaa agagttagat
    16201 gaatcagcga ttattgacgg atgcagcaag tatagtgtct ttaccaaaat tatttttcct
    16261 ttgctgaaac cttcgatcat cacgacgatc atcatccagt tttattggaa atgggacgac
    16321 ttcatgggac cattgattta tttaaacaag ccgagatctt acacggtatc gattgccatc
    16381 aaattatttg cagacgctgg atctagtacg acttacgcga gtatgtttgc gatgtctacg
    16441 ttgtcgctgc taccggtttt tctgattttc ttattcttca ataaatatct tgtccagggg
    16501 atcagtacaa gcggtctgaa gggctgaata tgaaaaagcc aatagaagag atgtttcttt
    16561 acaagctcaa ctactatgtc tgtcggattt ttttaaccag tcttatcagt aatgctcttt
    16621 tcgctttagt tctaggatgt ttgttcactt taggattagt gctgtctgct cgtgttgtct
    16681 atctgattta ttttttgatg atgtatcctt cttattatct atctgtccat tacttggcag
    16741 aaaaaaaggt atgttggagg aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt
    16801 ttcagctgtt tgtcattatt tttgcagttg attggtattt attagtggga aagattggag
    16861 cttggtatct gattcctttt ctattgtgtc tgttgctttt tgtatcccat agttttttta
    16921 gtacgctaat tgatatctgc aaagaagata aagcagatag tcttgtttat ttgatcaaaa
    16981 ggaatatctg gctgttgtca aaaaacgtca aggggtttct gtggtattta tgccttttgt
    17041 atggactgtt ctggtgtata cagcatgatt ttttactcac tgcattactg tatccagggc
    17101 tgccatttgt cacagtattt tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa
    17161 atatgtacaa gtcggacttg gtgggagagc gcgttttttt tatgaggcaa ttgcctctac
    17221 gtatcatcaa acttgtgaac tagtcggttt ttgtgatgta tctcaaacaa ggatggatta
    17281 tgcaaatcgg atacttgtga acaagtatgg acagaaaaaa gtaaagacat gtatgccatc
    17341 acaatttgag caactactgg atgacgtacg gccggctgaa gtcattatta cgtcagtaga
    17401 tcgaacgcat catgattata tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga
    17461 aaaacctctg actatcgatg aggaaaaagc tcaagaaata ataacggtcc aaaaacagac
    17521 gggaagaagc ctgcgagtca cttttaatta tcgttatgca ccgtatcaca cgaagataaa
    17581 ggaactgata gaaagtgatg tgattggtga tgtgttttct atccacttcg aatggctgtt
    17641 aaatacgcaa cacggggcag attatttcag aagatggcat cgaaataagc acaatagcgg
    17701 cgggcttctt gttcataaat ctacccatca ttttgatcta gtgaattttt ggttgaacag
    17761 ccgtccccaa aaggtatttg cattcggtgg attaaacttt tatgggatgc ataatgcgga
    17821 gaaacgcgga gtagaaatat tttacagtcg atgctatgga agcgaactag ctaaagctga
    17881 tccatttgct atagacctgt cagccaatga ggaattgcga gaaatgtatt tagaagcaga
    17941 aaaagacagt ggatatattc gtgaccgcag tgtattttcc gatgatatct ctattgaaga
    18001 tacactaggc gtaacggtca aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc
    18061 gtatatgccg tgggaaggat tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat
    18121 gaaagtgatc gaaaagtctt acatcaatgc aggtggagat aaaaaggatg aaggagcagc
    18181 cgttcttcag tccatccatg tctatccgat gttcggtgag ccatatgagg taatcgtcga
    18241 acaagaagcg ggcggacatg gcggaggaga tccgcgttta ttagatgatc tatttggtga
    18301 agaaaaagaa gaagatccgt ggaatcgggc agcaactcac gtagatggta ttttgtcgat
    18361 tttgacagga atcgcagcaa atcattctat tgcttcagga aaagcagtag tgattgacga
    18421 gcttgtcagc ttaaaagaat aagaatagat ttacggaaat tacttcccgt agtttataaa
    18481 atcttgcctt attgccgata ggctgtcttt tgaacactat ttattcggaa aaaggggcta
    18541 tgacatgctt tttgtcatgc cttcttttta tgtgggctgc ttttttagat aggagaaaat
    18601 aaaaataact atttaaaccc gaacaaaaag gaataatgca ttaaaaatca ttagatttta
    18661 caaaaaaata agtaaaccac gttgtcagtt tcagaaagta gtgttagact gttttcaata
    18721 tttgaaacaa cattttttat tcaaatatgg caaactgcct tcacgtgcaa aacagttgca
    18781 agaaaatgaa tttacactat tattagtttt acatacctac ttatatatcg tcataatatg
    18841 gatgacagtt tctagccagt accgtaaatg ctggactata agtaaaagat tggctatttc
    18901 acccttcttt tcttatgata aagtggaggg tgtttttctt taacagaaac atagtaggtt
    18961 tttgcacaaa agcggttatt tcggtattaa taaatgataa aggagcaaat aaagtgaaag
    19021 aattagttga acgtattcaa aaagacggac gtatattagg cgagggcgta ttaaaagtcg
    19081 acagcttcgt cactcaccaa gtagaccctg tattgatgga acaaatggga aaacggtttg
    19141 cagaagtatt taaagaacaa ggaatcacga aagttgtgac cattgaagct tctgggatcg
    19201 caccagcttt atttgctgca agagaattag atgttccaat gatcttcgct agaaaagcaa
    19261 aaagtttgac gatggatgaa gaattattga cagcttctgt ttattctttt acgaaacaag
    19321 tgacaagtac gatttctatt tctcgtaaat ttttatcttc agaagacaaa gtattgatta
    19381 tcgacgattt cttggcaaac ggacaagcgg caaaaggatt gatcgaatta tgccagcaag
    19441 ctggagcgca agtagaagga atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc
    19501 agttacttga agaaatggga ttgagagtcg tttctcttgc gcgtattgct tccttgacga
    19561 atggacaagt tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg
    19621 aaaagctgcg gttttagggc tgcagcatct gctagcgatg tatgcaggag cagtagcggt
    19681 gcctcttttg atcggtacag gactaggctt taatcaagaa cagatgactt atttgatttc
    19741 aattgatatc tttatgtgtg gattagcgac attattgcaa ttgacagtta accgattttt
    19801 cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc cactgatctt
    19861 gatcggaacc aatgaaggag taggagctat ctacggatct attatcgctt ctgggatctt
    19921 tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa ctattccctc ctatagtaac
    19981 aggaacggtt atcaccgtta ttggtttgac tttgattcct gtagcaatcg agaaaatggg
    20041 tggaggtaat gcaacagcag ctgattttgg tgacaaaaca aatctgctac ttgcatttgt
    20101 gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc
    20161 ggtattgatt ggtttagtcg gaggaagcat cttagctgct ttcatgggaa tgattgatct
    20221 gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa cccttttatt ttggtaaacc
    20281 aactttcgac gtatggtcga ttttactaat gatcatcatt tccatggtca gcatggtaga
    20341 atcaacaggt gtatattttg cacttggtga cattactggg aaaaatgttg gagaagaaga
    20401 attgaaaaag ggatatcgtg cagaaggact tgccgttatc ttaggcggta ttttcaatac
    20461 attcccttat actggctttt ctcaaaatgt cggattggtt caactttcgg gaatcaaaac
    20521 aagaaaaccc atctatttct ctgcattctt cttgattgtt ttaggattat tccctaaaat
    20581 cggtgcgtta gcacaaatca tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt
    20641 cgggatggtg gcagtccaag gaatccgtat gctgttggaa gttgatttca gcaatgataa
    20701 aaatctattg attgttgctg tttctgttgg atttgggctg ggtttcaatg ttacgccgac
    20761 attattcagt caaatgccgc aaacattgca aatgttcact ggaaatggca tcgtaatgag
    20821 tagcattacg gctatcatat tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat
    20881 attggaagaa gccttcaaga agtaatagag caaaagaaag gttgtgaaat cagcgttttg
    20941 acctgattgt tagagaaaca aacggaaaaa tagttcctcg tattttgccg tttgtttgag
    21001 ctaataccga gggtcagctg attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt
    21061 catctccgag tcacaaggaa caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt
    21121 tccttgtgac cgaggagttg gcaggagaac accgtttatc cataaaaagg aatgtgacct
    21181 aacttttgtc acattccttt tttttatctt taataaacac gaacaataaa atgtttttct
    21241 ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag
    21301 aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag
    21361 attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa
    21421 aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc
    21481 gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta
    21541 tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa
    21601 atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta
    21661 cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca
    21721 tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg
    21781 ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc
    21841 gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa
    21901 gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcacatt
    21961 gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt
    22021 acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt
    22081 attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt
    22141 ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc
    22201 caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat
    22261 gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt
    22321 ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc
    22381 atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac
    22441 cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag
    22501 gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg
    22561 gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct
    22621 cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt
    22681 cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg
    22741 gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac
    22801 tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc
    22861 actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg
    22921 gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg
    22981 acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg
    23041 gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt
    23101 gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca
    23161 cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt
    23221 acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg
    23281 gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca
    23341 gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt
    23401 gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga
    23461 gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt
    23521 gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatttctac tcaagttctc
    23581 cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa
    23641 cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt
    23701 tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca
    23761 gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat
    23821 gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct
    23881 gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta
    23941 acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc
    24001 cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt
    24061 gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa
    24121 gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat
    24181 catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga
    24241 gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc
    24301 gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt
    24361 ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg
    24421 taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg
    24481 agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata atgatttttg
    24541 aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa
    24601 agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat
    24661 gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta
    24721 tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt
    24781 gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa
    24841 tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga
    24901 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
    24961 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
    25021 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
    25081 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
    25141 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
    25201 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
    25261 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
    25321 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
    25381 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
    25441 tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga
    25501 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
    25561 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
    25621 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
    25681 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
    25741 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
    25801 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
    25861 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
    25921 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
    25981 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
    26041 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
    26101 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
    26161 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
    26221 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
    26281 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
    26341 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
    26401 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
    26461 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
    26521 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
    26581 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
    26641 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
    26701 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
    26761 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
    26821 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
    26881 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
    26941 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
    27001 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
    27061 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
    27121 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
    27181 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
    27241 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
    27301 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
    27361 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
    27421 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
    27481 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
    27541 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
    27601 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
    27661 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
    27721 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
    27781 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
    27841 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
    27901 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
    27961 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
    28021 atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag
    28081 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
    28141 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
    28201 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
    28261 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
    28321 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
    28381 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
    28441 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
    28501 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
    28561 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
    28621 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
    28681 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
    28741 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
    28801 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
    28861 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
    28921 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
    28981 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
    29041 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
    29101 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
    29161 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
    29221 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
    29281 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
    29341 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
    29401 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
    29461 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
    29521 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
    29581 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
    29641 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
    29701 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
    29761 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
    29821 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
    29881 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
    29941 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
    30001 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
    30061 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
    30121 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
    30181 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
    30241 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
    30301 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
    30361 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
    30421 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
    30481 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
    30541 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
    30601 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
    30661 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
    30721 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
    30781 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
    30841 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
    30901 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
    30961 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
    31021 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
    31081 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
    31141 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
    31201 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
    31261 atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag
    31321 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
    31381 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
    31441 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
    31501 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
    31561 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
    31621 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
    31681 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
    31741 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
    31801 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
    31861 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
    31921 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
    31981 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
    32041 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
    32101 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
    32161 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
    32221 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
    32281 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
    32341 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
    32401 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
    32461 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
    32521 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
    32581 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
    32641 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
    32701 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
    32761 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
    32821 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
    32881 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
    32941 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
    33001 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
    33061 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
    33121 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
    33181 ccagcagaag caaaagcatt agtgtttgct tggagagctg tcaaacatgt gaaatctaat
    33241 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
    33301 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
    33361 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
    33421 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
    33481 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
    33541 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
    33601 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
    33661 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
    33721 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
    33781 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
    33841 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
    33901 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
    33961 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
    34021 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
    34081 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
    34141 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
    34201 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
    34261 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
    34321 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
    34381 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
    34441 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
    34501 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
    34561 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
    34621 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
    34681 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
    34741 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
    34801 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
    34861 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    34921 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    34981 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    35041 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    35101 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    35161 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    35221 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    35281 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    35341 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    35401 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    35461 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    35521 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    35581 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    35641 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    35701 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    35761 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    35821 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    35881 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    35941 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    36001 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    36061 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    36121 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    36181 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    36241 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    36301 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    36361 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    36421 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    36481 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    36541 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    36601 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    36661 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    36721 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    36781 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    36841 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    36901 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    36961 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    37021 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    37081 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    37141 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    37201 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    37261 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    37321 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    37381 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    37441 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    37501 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    37561 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    37621 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    37681 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    37741 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    37801 tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac
    37861 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    37921 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    37981 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    38041 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    38101 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    38161 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    38221 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    38281 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    38341 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    38401 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    38461 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    38521 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    38581 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    38641 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    38701 gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc
    38761 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    38821 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    38881 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    38941 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    39001 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    39061 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    39121 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    39181 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    39241 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    39301 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    39361 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    39421 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    39481 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    39541 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    39601 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    39661 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    39721 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    39781 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    39841 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    39901 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    39961 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    40021 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    40081 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    40141 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    40201 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    40261 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    40321 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    40381 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    40441 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    40501 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    40561 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    40621 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    40681 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    40741 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    40801 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    40861 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    40921 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    40981 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    41041 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    41101 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    41161 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    41221 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    41281 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    41341 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    41401 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    41461 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    41521 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    41581 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    41641 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    41701 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    41761 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    41821 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    41881 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    41941 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    42001 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    42061 cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt
    42121 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    42181 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    42241 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    42301 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    42361 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    42421 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    42481 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    42541 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    42601 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    42661 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    42721 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    42781 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    42841 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    42901 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    42961 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    43021 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    43081 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    43141 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    43201 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    43261 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    43321 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    43381 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    43441 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    43501 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    43561 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    43621 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    43681 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    43741 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    43801 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    43861 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    43921 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    43981 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    44041 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    44101 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    44161 gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga
    44221 ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt
    44281 ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg
    44341 ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga
    44401 agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac
    44461 ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat
    44521 tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg
    44581 gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac
    44641 ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga
    44701 atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat
    44761 tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt
    44821 tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg
    44881 gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg
    44941 gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg
    45001 tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat
    45061 gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg
    45121 aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa
    45181 tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga
    45241 aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat
    45301 tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa
    45361 aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc
    45421 caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc
    45481 ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat
    45541 gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata
    45601 gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat
    45661 cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt
    45721 caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc
    45781 catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg
    45841 tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg
    45901 atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag
    45961 atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg
    46021 aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat
    46081 cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa
    46141 ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg
    46201 attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt
    46261 taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa
    46321 gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg
    46381 cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa
    46441 aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg
    46501 gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat
    46561 gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg
    46621 ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg
    46681 atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa
    46741 atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat
    46801 tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg
    46861 gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt
    46921 gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg
    46981 gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca
    47041 gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc
    47101 tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt
    47161 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
    47221 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
    47281 gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
    47341 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
    47401 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
    47461 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
    47521 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
    47581 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
    47641 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
    47701 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
    47761 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
    47821 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
    47881 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
    47941 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
    48001 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
    48061 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
    48121 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
    48181 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
    48241 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
    48301 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
    48361 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
    48421 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
    48481 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
    48541 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
    48601 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
    48661 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
    48721 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
    48781 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
    48841 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
    48901 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
    48961 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
    49021 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
    49081 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
    49141 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
    49201 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
    49261 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
    49321 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
    49381 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
    49441 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
    49501 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
    49561 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
    49621 atggcttatc aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
    49681 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
    49741 aatgacaaat cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt
    49801 gttccagaag gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga
    49861 cacgaagaca atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg
    49921 gctgctgaac ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa
    49981 tttaagttac cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc
    50041 aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca
    50101 gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc
    50161 actgtagcaa atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca
    50221 ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca
    50281 gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt
    50341 caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc
    50401 agagtaacaa aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg
    50461 aaatcagaag cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa
    50521 ccagctgcac cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca
    50581 atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca
    50641 cacgtgacat tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt
    50701 aaagaagttg ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct
    50761 ttaacagcga ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa
    50821 gaaatcgttt ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta
    50881 tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc
    50941 aatgagaaag caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga
    51001 acaattacga tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac
    51061 tatccagaag tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct
    51121 gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc
    51181 gtagatggag caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca
    51241 gagctattaa tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt
    51301 agatacagtt gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga
    51361 aatgggacaa aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt
    51421 cggctgtatt ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga
    51481 ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga
    51541 atggaaagaa aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa
    51601 acataaggtt gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt
    51661 cattcatcca gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc
    51721 ccgtccaatc gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg
    51781 attagcattg aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc
    51841 cgaattgggc ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc
    51901 acaaatttta ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa
    51961 aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag
    52021 cgtgacagtg aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat
    52081 ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga
    52141 agtaggagaa cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat
    52201 ctatgccatc ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc
    52261 aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc
    52321 ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa
    52381 agatgctggt atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt
    52441 atcgcttggt aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct
    52501 tattggcgcg caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc
    52561 aatcgaatca ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct
    52621 tggcgaaatc gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt
    52681 tgaataatac aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc
    52741 aataatatta ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta
    52801 tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca
    52861 cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg
    52921 gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt
    52981 aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct
    53041 tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg
    53101 agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag
    53161 aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc
    53221 gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc
    53281 attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg
    53341 atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg
    53401 aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt
    53461 tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata
    53521 aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg
    53581 tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca
    53641 tggggacacg tatgtacgga tgagctggtc ttgaactagt ggcaatcttg aaaggaaacc
    53701 acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg
    53761 aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg
    53821 aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag
    53881 aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat
    53941 tttctcgaaa aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc
    54001 agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa
    54061 ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac
    54121 attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga
    54181 acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa
    54241 cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga
    54301 cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga
    54361 tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca
    54421 tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt
    54481 cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt
    54541 tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga
    54601 tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt
    54661 cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt
    54721 aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc
    54781 attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt
    54841 tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg
    54901 tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc
    54961 tatccttcac attgacgaac caacattgca aatgactttc ttagttaata attcaccatt
    55021 tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga
    55081 acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt
    55141 ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata
    55201 cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga
    55261 accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc
    55321 cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg
    55381 tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat
    55441 gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg
    55501 tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac
    55561 ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt
    55621 atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac
    55681 gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa
    55741 aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg
    55801 cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga
    55861 aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta
    55921 taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc
    55981 aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat
    56041 ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc
    56101 ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta
    56161 aaacaatact ttggctatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc
    56221 attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac
    56281 cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg
    56341 atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc
    56401 tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt
    56461 ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga
    56521 tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat
    56581 ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta
    56641 attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta
    56701 aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa
    56761 gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat
    56821 caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt
    56881 aaaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca
    56941 gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt
    57001 ggtatgggta gaaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct
    57061 ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc
    57121 atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa
    57181 agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg
    57241 tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg
    57301 tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca
    57361 cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg
    57421 aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct
    57481 acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg
    57541 atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa
    57601 agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc
    57661 cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg
    57721 gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa
    57781 gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc
    57841 caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct
    57901 aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa
    57961 tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga
    58021 ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag
    58081 cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca
    58141 aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg
    58201 ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac
    58261 gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg
    58321 gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat
    58381 cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc
    58441 gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt
    58501 ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa
    58561 aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt
    58621 gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat
    58681 ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa
    58741 aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt
    58801 tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt
    58861 tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt
    58921 aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg
    58981 aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc
    59041 tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa
    59101 cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga
    59161 agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat
    59221 tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc
    59281 aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt
    59341 atttgtcaat cttggaggaa ttactggcgt tattcctttg acagggatca cgttcccatt
    59401 tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa
    59461 catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg
    59521 gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg
    59581 cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg
    59641 tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg
    59701 cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc
    59761 atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag
    59821 gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag
    59881 caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg
    59941 cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg
    60001 cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag
    60061 agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg
    60121 ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg
    60181 gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag
    60241 tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag
    60301 ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg
    60361 aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca
    60421 cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg
    60481 atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca
    60541 ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa
    60601 tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg
    60661 cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg
    60721 ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg
    60781 gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag
    60841 gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac
    60901 gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag
    60961 gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca
    61021 caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg
    61081 tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc
    61141 atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga
    61201 ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg
    61261 tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa
    61321 tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt
    61381 atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat
    61441 ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac
    61501 gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa
    61561 gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg
    61621 gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc
    61681 gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata
    61741 ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg
    61801 acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt
    61861 atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa
    61921 accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc
    61981 ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac
    62041 aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg
    62101 tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata
    62161 tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa
    62221 cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg
    62281 gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac
    62341 gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa
    62401 aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct
    62461 ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa
    62521 catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa
    62581 cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt
    62641 tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta
    62701 cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt
    62761 ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt
    62821 tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag
    62881 tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag
    62941 tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct
    63001 gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact
    63061 tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca
    63121 ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa
    63181 ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta
    63241 tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac
    63301 tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat
    63361 gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac
    63421 agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga
    63481 taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt
    63541 gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct
    63601 tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg
    63661 aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca tatacaatca
    63721 aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga
    63781 ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac
    63841 gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt
    63901 agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata
    63961 ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg
    64021 cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag
    64081 tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg
    64141 ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg
    64201 aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg
    64261 aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc
    64321 gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa
    64381 cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg
    64441 aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag
    64501 gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc
    64561 gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat
    64621 gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca
    64681 gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat
    64741 atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc
    64801 gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg
    64861 agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta
    64921 tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc
    64981 aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac
    65041 agataaagta aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt
    65101 attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag
    65161 tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga
    65221 aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga
    65281 acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa
    65341 acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc
    65401 agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca
    65461 tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa
    65521 tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat
    65581 acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact
    65641 tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct
    65701 catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa
    65761 gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa
    65821 cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta
    65881 gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc
    65941 ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa
    66001 tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat
    66061 cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga
    66121 tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct
    66181 tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc
    66241 ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa
    66301 tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa
    66361 acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa
    66421 aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa
    66481 aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac
    66541 aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga
    66601 gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga
    66661 atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat
    66721 gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat
    66781 tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa
    66841 aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat
    66901 ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc
    66961 tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc
    67021 gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga
    67081 gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga
    67141 aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa
    67201 ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg
    67261 atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg
    67321 atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga
    67381 gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat
    67441 ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat
    67501 atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat
    67561 gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt
    67621 tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca
    67681 gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct
    67741 cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga
    67801 tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg
    67861 gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc
    67921 gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg
    67981 gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat
    68041 aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct
    68101 ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca
    68161 agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat
    68221 tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa
    68281 aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt
    68341 atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa
    68401 gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt
    68461 atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg
    68521 gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt
    68581 aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata
    68641 taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat
    68701 aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta
    68761 tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg
    68821 atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg
    68881 tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta
    68941 cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca
    69001 tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat
    69061 gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac
    69121 caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat
    69181 gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat
    69241 gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat
    69301 ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag
    69361 cataacggta tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac
    69421 atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat
    69481 ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga
    69541 agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg
    69601 cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt
    69661 caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca
    69721 aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc
    69781 tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc
    69841 aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct
    69901 atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc gacgtatcgg
    69961 atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat
    70021 tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt
    70081 caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct
    70141 tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag
    70201 ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt
    70261 cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt
    70321 aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt
    70381 gttatttctc acactcttca atcgctggct gccggaaact ttacttttgt tgatagaaga
    70441 gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg
    70501 acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag
    70561 gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt
    70621 gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga
    70681 cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca
    70741 gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa
    70801 tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca
    70861 cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa
    70921 aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc
    70981 tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta
    71041 tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg
    71101 tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat
    71161 ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca
    71221 tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg
    71281 gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac
    71341 actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg
    71401 ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc
    71461 atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt
    71521 tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt
    71581 ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg
    71641 acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagctgaag
    71701 cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt
    71761 taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg acggcttatt
    71821 tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg
    71881 aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt
    71941 tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa
    72001 tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga
    72061 tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag
    72121 aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg acccaatata
    72181 ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg
    72241 aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa
    72301 aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc
    72361 catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac
    72421 agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag
    72481 aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag
    72541 aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa
    72601 aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc
    72661 gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac
    72721 agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt
    72781 tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct
    72841 tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt
    72901 aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg
    72961 ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt
    73021 cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat
    73081 agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt
    73141 tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat
    73201 acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta
    73261 tcctttgatc agtaaaggct attctggtgt accgattatt ttaatgtatt catcgatatt
    73321 ttttatcaac atttttttta tgaccaactg gttccgacgg ttccatttgc cacttacttc
    73381 cagcacatat ttatttaggg gcaaacgtaa gatatatttt gccaaatagc gatctttaag
    73441 caggccattg atcacacggt cctcaagctg caagttgatc ccagtcagca tcattggctg
    73501 ttcttgtaaa acgtacgtat caattaaaag accttcaaaa gtttccacaa gcgtcttcct
    73561 ttcttttcaa agtaacctga aaacaggcac taattcttcc attttgatat tcgttgaagt
    73621 ggatgcgatc attctttctt ttgtccactg taatctagta taacaaaaaa gagcagaaga
    73681 gccaaaaaga aaaaagagca tcgaattcta taggaagtct cattggcacg gcctaaatta
    73741 ttacaaaaca ttgattaaaa aaagcccctg catcgcagga gcatccccta tatcgtttta
    73801 ataggtcaat caaaaagcag tatcaaacac attttgtatc aaatcaggaa ataatagata
    73861 agctaagccc agaatcaaaa gtaccgctag aagaaaccag ccgattgttc ctatgaaata
    73921 ctttaatttt tcgaacattg tatcacctcg ttgtcagtat atcacggatc agtaaattgg
    73981 gggtgttaag aatctcctaa aatagacaaa atttgttcta ttttcctaaa gcaaatactt
    74041 gtaagataca gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga
    74101 agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa
    74161 tcaatcctac acccttggaa gataagacag ccactagccc accgcaaaaa acagctaccc
    74221 agccaatagg ggatttaaag gcttctaata gatcacgtag accgatttct cctgttgcga
    74281 tagggattag aatagccgct gtgatgatcg tcaccccaat attgattcct ttgccttgaa
    74341 gccaagggaa aaaggaacgg gaggctggta tgagcttcat aatcaaaaca aaagctgccg
    74401 ctatcatcag actttgattt ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa
    74461 gccaactttc catgaaatat attcctcact ttcttcgaaa caatcataac atgtttttag
    74521 aaagttacaa aagtaggaat tcatccttca agacctattc ataagctgtt tttttcataa
    74581 aataaacaaa atcataaaaa aacactttgc ttatttgaca gaagacagcc gcgctttgta
    74641 tagtggacaa gacttacatt tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa
    74701 acgcaagcca ttcagaataa ccaatattgt gcttccttcg tgtcctacaa ctccaagagg
    74761 tagatttaaa aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa
    74821 aaataatatt ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa
    74881 atttatttaa gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc
    74941 cttgccccat agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat
    75001 caccaaccat cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt
    75061 cttccggagt acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg
    75121 ctgtgccaga atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg
    75181 aaattacttg ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact
    75241 gctgatcttt agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt
    75301 ccgcaatctc tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc
    75361 attgcgcacc taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt
    75421 attggatttc ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat
    75481 gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga
    75541 aaaagctgtc agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa
    75601 tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt
    75661 ttgcagaatt tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag
    75721 gagaagccac aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta
    75781 ggaaaaagtg ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat
    75841 tattttcgat tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta
    75901 ctaattggat aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga
    75961 tgggatttcc ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag
    76021 gcacagattc accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc
    76081 catctatcgg aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag
    76141 aaacatctac ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa
    76201 gattcatcaa agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac
    76261 cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg
    76321 caccaatagc agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt
    76381 ctttcaagcc ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa
    76441 gtggggtgat ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc
    76501 agccgattgt tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc
    76561 aaaacaagga tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat
    76621 aatggttatc atttggtgat taagcttgat gttgttttta ttatttataa tgattattat
    76681 taaagaaaat aaaaaaccag aacatttctc aaataagaga agtgttctgg ttttttctac
    76741 attgcaataa accaataacc aatcaaaatg ataccaagga taatacgata ccagccaaaa
    76801 gcggtaaagt cgtttttctt caaatagctt agtaagaatt tgatagctaa gacggaaacc
    76861 acaaaagata cgaatgttcc tacaagaaga acaacagttt cttccatttt gaatgaattc
    76921 ccttcagcta aatatttgac gatcttcaag ccgcttgcgc caaacatgac aggaatccct
    76981 aagaaaaatg aaaactctgt agcgacaaaa cgcgaagcac caatcaagat agatcctagg
    77041 atcgttgcgc cagaacggga tgttccggga acaagtgcca agacttggaa taacccaaca
    77101 agaagagctg cttggtacgt aaagtcattc agagaagtcc aacgaggtgt tttgtttttg
    77161 ttgcgttttt cgacaataat aaaggcaata ccgtaaacga tcaaaacaat ggcaactggt
    77221 aagaacttgt ggaaatttgc ttctaaccaa tcatccaaag gcaaaccaat gatgatggct
    77281 ggtatgcaag caacgatcac tttaaaccaa agtgtccacg tatctttttt ttctaccgtt
    77341 gatttacttg gagaaaatgg gtttaacttg tgaaaataaa gaacgacgac agccaagatc
    77401 gcccctaatt ggataacgac attgaacatc gacataaatt gtgaactggc atccagtttg
    77461 atgaattcat ctgctaggat caaatgacca gtactactga tgggcagcca ttccgttatt
    77521 ccttcaataa taccgagaat cactgctttg ataatatttg caaaaaacat aaattccttc
    77581 cttcccaatt aagatacatc ttatagtata ccgttatttt ttgagaaacc caatctgttt
    77641 tttaatatta agaaaaactt gagaaataaa gcagataagt acacgaagag aaaaaagaag
    77701 acaaagccct cgttcagact ttgcctttta tgattctctg gtaaaaatta aaatatcccc
    77761 attatagaaa cgagtcgcga ctatgtgttt tcccgttatc gatataagac gtatcattga
    77821 tgccgtggat agaaaatcga ccgtttgcat aagtgacctt tgttacgctg ccatttaaaa
    77881 gcccagcacg aacaggctgt ttaggatcga tcagattaag caaaaaggcg atcgtcaaac
    77941 catgagatac gactaaaaca tttccaccac catttttttc gcagtgatgg gcgatatctt
    78001 caaatcctgt ccatacacgg tctcgcaatg tttcataagt ttccgcccac ccagcggtat
    78061 ctaagttgta tacagcatta gcaatttgtt caaaagaaac ttctgtggta aacatttcat
    78121 catatgtttt aaaattcaat acacgaggga tcacacccca catttccatg tcatatccac
    78181 cttcaagaga cccgaaacac cattctctaa tccttttttc aataaaataa ggaatttctt
    78241 ttcccttaga atgttctcct agaataatac gggcagtttc gattgcacga ccactgtcac
    78301 tagaataagc aaatgaaaag tcgacatttt ttaacccgcg acctaaataa tgaatgcctt
    78361 cttgtccttg ctttgtgagg ggtgtatcac accagccttg tactcgttca attgtattga
    78421 acatcgtttt tccgtgtcgt acgatataaa gtgtagtttc tgtcatttta ttctcctcct
    78481 aagtcgtttt aacgattgaa aaaaggattt gtcctttttt cctgttcaat cgtcgttgca
    78541 tcgccatgtc ctggatagac tgcaaattca tcaggtaata caaacagctg ggtccgaata
    78601 ctatgaagta attgttctaa attccctgta tacagatcgg ttctaccgat acttcctcta
    78661 aacagggcgt cgccagaaac gacaaaatca tcaaaaataa agctgacgct tcctatagaa
    78721 tgccctgggg tagggacgac gcgaaaagac atgtttccta aagtataagt tttcatttca
    78781 aattcatatt ctgcaggcga gacaataaca tcagcaatat catcatgtct accgagtcca
    78841 gaaagattca aaatcggatc acctagccat ttttgctcta gtgggctgac ataaactggg
    78901 atttgataat acgcgcgtaa aggttccact gcaccgatat gatcgtagtg agtatgggta
    78961 agcaaaatcg ctaccggctg ttggtttgtt tttttgattt cttgtttgat tcgttccgca
    79021 tcttctccag ggtcaatgat caaaagtgct tcttcgttgt agaccaaata acagttttct
    79081 tcaatcattc ctgtttttaa tctttcgata tgcatgtgca ttcctccttg atagccaaaa
    79141 cttctttcat ccattagtat aacgcattca ttgctaggaa acaaaaacag gtacttattt
    79201 ctctttactt atctgaatta tcagactata tgaaaagtat ttggatattc taaagagaag
    79261 gaaagtaagt tttcatagca ttttaatatg tccttgttac attagattca tccaaaaatc
    79321 caaattctat atcctatatc cctgcctaat cagcagggtg tttttttatg tttggaaaga
    79381 gagctctttt tgctaaacta ggttttttca ggtaatctta gagagaaaga ggtggtggaa
    79441 atgatcaaac agtcaaaaat atctgtgcgg cacttagtag aatttatttt gcgacgtgga
    79501 agtatcgaca atcgtaaaaa aagtaaccat actgcattag aaggagcgag aatccaccgc
    79561 aagctccaaa aagatgcggg agaaaactat gaaaaagaag tctttttaaa aacaacagtt
    79621 caattagatg aatattacct gatcgttgaa ggccgtgcag acggtatttt caaaagagac
    79681 ggtatttatt atatagatga aatcaaaacc tccgaaccac gatttgaaga tttagaacca
    79741 gaacaagtgg aacttttctt tcatcaagca agggtgtatg cctatatcta tagtcaagaa
    79801 catgatttac aagaaataaa tctgcaattg acttacttcc agacggcaga agaagtgatc
    79861 acgagaaagg ttgaacatca gacaagagag cagttagaaa ctttttttaa aaagctgact
    79921 gaagattata aagaatggct tgtttttcaa gaaaactggc gcactgtacg caatgcctct
    79981 ttgatggctt tgaaattccc ccatgatgag tataggaaag gtcagcgtga actagcggta
    80041 gctgcttata aaacgatccg tacgaaacag aaattgtttg tagaagcacc taccggaaca
    80101 ggaaaaacaa tctctacgtt attccccgct ttgaaagctg taggggaaga agagggagaa
    80161 aagatctttt atctaacggc taaaacaata actagacaag tagctgaaga tgctatgaca
    80221 gcattaaaag atacaggagc tgaagtaaaa agtgtcactt tgacggcgaa agacaagatc
    80281 tgctttttag atgagacgac ctgtaatcca gatcagtgcc catatgccaa tggttattac
    80341 aatcggatta atgaaggttt atgggatctt ttgaatcatg agaatcagat cactcgtgaa
    80401 gtcatcgaaa cttatgcaag aaagcataca ctttgtcctt tcgaactatc attagatgtc
    80461 agtatctggt gtgacgtaat cattggggat tacaattatt tatttgatcc aactgtttat
    80521 ttaagacgtt tttttgaaga tgaaaaaaac gaggactatc tgtttttgat tgacgaagca
    80581 cataatttgg tgaatcgttc ccgagagatg tattcggcag aactatctta tgaaaaaacg
    80641 aaacgaagta aggaagctat accaaaggaa ttcaaaaaac ttcatcgccg tttcaacaaa
    80701 ctattaaaag aatttgattc gatccgagaa atagcaaaag aagatcattg ggattatcat
    80761 catcaaaaag caccggctga atctttagta aaggcaggtt atcaattaag cgaaaaaatt
    80821 aaagaatggc tggcagaatt tcctgagcac ccatcacaag aacaattgct tccttattat
    80881 tttgatttgc ttcatttttt gaaggtcagt gaattttatg atgatcacta cgaaacaaca
    80941 gtcgaaaaaa cgtatcgcga tctgatagta aaagagtttt gtattgatcc aagcttgttt
    81001 cttgagcagt cgttggataa aggaaaaagc agtattcttt tttctgctag tttttcacca
    81061 ctctcttatt atcaggaaac actgggggga caaaaaagtc tggcttacaa actgccgagt
    81121 cctttcccag aaggaaacca gcaagttttc atcgcaaatt acctagagac gacttatcga
    81181 aaaagggaac acagtctggc aaaattagta gagacgattc atgcttttgc tgatggaaag
    81241 gtcgggaatt atttcgtttt ctttccttct tatcaatatt tagatcttgc ggtggaagca
    81301 ttcagacagc aatatcccga cagcaatgtg ctaatccaag aaacggacat gaatgaggaa
    81361 gaacgcgaac gatttttaac aaaatttcaa acaaatcccg ctgaaacatt gattggattt
    81421 tgcgtacttg gcgggatttt ttcagaagga atcgatttga gaggggaccg attgatcggc
    81481 agtatgatcg taggtgtagg tttgccgcag atgaatcatg agcaggaact gatcaaatca
    81541 tattatgatg agaaggaaca ccttggattt gcttacgctt atcagcttcc tggaatgaac
    81601 aaagtcttgc aagctggtgg tcgagtgatt cgtggcatgg cggatcaagg aatcgttgta
    81661 ttggcagatc aacgattttc tagtttttct tatcgacgtt tattcccaca acattggcaa
    81721 acagcaagag aagtacgaag tgttggggaa ttggaagaag gaatacagcg tttttggtta
    81781 tcaaaagaag cagtaaatca aaaaaacgag gaatgacaga cgtcagcctc gttttttgat
    81841 taacgatagt tagtgaattg caactcgatt ggtaagtcaa gtttacgtaa taaagcaatc
    81901 acagcttgta gatcatcacg gttcttacct gtcactctaa tctgatcttc ttggatttgt
    81961 gcttttactt tcagtttttc atttttgata gctgtagtga ttttcttcgc gttttcacga
    82021 tcgattccgt tgaccaaatc ggcagtttgt cgagctttgc cacctagagc gtgttctgat
    82081 tcggaaaaat ggatattttt gattggcaca tttcttttga tcagcttacc aaataataca
    82141 tctttgatct gttcgatttt gaaatcatca tcgctcaata caactagatt gctgttctct
    82201 aatttgattt ctactgtaga atccttgaag tcaaagcgat tggacaattc ttttgttgtt
    82261 tgctgaatcg cattttttac ttcttctgta ttgacttctg atacaatgtc gaagctagca
    82321 tcttttgcca taggaaccct cctgatatac atataaatat ttaccataca tctatcttat
    82381 cataggatta gggcagattc ttacctgttt gctaaaattc ttctcatttg agttcgaagt
    82441 attttatttt aatagataaa ctgttattct tacatttgga ataccaaagt aggggagagg
    82501 tgcttgtgag taaaattatg actgcgtatc ataaaaatga aagattaaag aaaatagtgg
    82561 ttattttgat aaccggattg accgctgcgg tcgctttaaa ttattttttg attccagcca
    82621 atgtcttttc tgcagggatg aatgggatcg ctcaaatcat ttcctcgctt ttatatgaat
    82681 ggtttcatat caagatagac acaggaacat ttatttttct tttgaatatc cctgtgtttg
    82741 tattagggtt tctcaaagta ggaaaacgag caacgatcct tagttttgtg aatgttgtat
    82801 gtgtatcggt gatgacgatg ctgcttccag tcggacagat cactgataat attttgatga
    82861 atgccctagt aggcggtgtt ttattaggtc ttggcgtagg attatcttta aaaatgggct
    82921 ttacaactgg tgggatggat atcatctctc ttgtactttc acaaacaact ggaaaaacag
    82981 tcggaaacta catgctgatt ctaaatggta tcattgtagt catcgcaggc ttcttcttta
    83041 actgggaaag tgccttgtat acgattattt ccatttatgc aatgactcaa gtaattgatg
    83101 ccatccacac gagtcatcaa aaagtaacag caatgatcgt taccgtcaag ccagaagagg
    83161 taacagaaga actagcaaaa cggatggtac gtggaatgac attactgccg tctgtcggtg
    83221 gctattcaaa aaaacaaggg aacatgatca tgatggtcat cacccgctac gaaatgtatg
    83281 atcttgatca gatcgttcat gaaatcgacg acgatgcatt tatcaatatt ttaccaactc
    83341 attcagtatt tggccggttt gcaaatgaaa acgaacaaaa aatgtatcga acaactggcg
    83401 tattgccaga aataaaaaat cgttcacaaa aaaaatgagg aatcaggttt attttagaat
    83461 aaatttaatc tattacatct aataagaaca tttttataaa tttaaacgag aatcttactt
    83521 ctaaattctc atttacaagc agctaaaaaa agagtatgat aaataaaaat cgtgttaagg
    83581 agtgtcgtac gatgagcgaa gcaaaccaag aacggtctgg acaagaaata gaactaatca
    83641 aagaacgtat tcttgctgcc ttggaaatgg tgattgatcc tgaattagga atcgacatcg
    83701 tcaatttagg cttgatttat gatatagaat tcaatcctga aaatggagaa acagtcatta
    83761 agatgactct aacaactatg ggctgtccat tagcagatat tttaacagaa tctatccatg
    83821 acgcattaaa agaagtgcca gaagtaacga aagcagaagt caaactagtc tggtatccag
    83881 catggacgac agataagatg agccgttatg cacggattgc gttggggata cgatagaaat
    83941 cttggctgac tagctatgag aagcaaatag gaaaaacaaa aaagcctttt atcctttcta
    84001 gaagaaaaga gataaaaggt ttctttgttt ataaaatttt gtctgctata ttataagtta
    84061 attaaataaa tgtgttctat tgataaagga cattcaaatc cctttccttt tggaatcctt
    84121 cttttttaca tcagtttgat tttcgtatag tattctttct catcttcacg ataaatcgca
    84181 gccccatttt tttgttgtgc tttaagactg ccaagatttt ttttatttac agacaaaagt
    84241 gcctcatcaa tgccgatttt ttttgcctct tgtagtacta atgctaatcc ttgtgtggca
    84301 tatccttttt tacgataatt aggggagatg ccgtaaccaa tatggccagc gccgtttctt
    84361 aatgtttcgt tcagtttatg gcgcaaatta aaaattccta catactcatg ttcatcactt
    84421 tctaaaataa agacggttgc gggaacgtaa ctctcaggaa gatcgattcc taaagacgtt
    84481 ttttcgcatt tttttacata atctaaaaat tcagcaaatg tatagccata agcattgttg
    84541 ataaatccac tctcatttgg tccaaagcta gtttgtagct gataagcttt ctttaaatct
    84601 gcttcccata gtttgatcag tttcatcgta ttcacctctt taagtaacct tattcatttg
    84661 ttttctacta tagcactatt gttgtaaata gccatgccta tttttattta ttgtgtcata
    84721 tcgtattaag aatcatcgaa caatacaaat atgaattaca ataaaaaaat tatttgaagt
    84781 tcgttcattc gttacttatg atctctagaa gaaagaaaac gtgaattact tcagcgtcta
    84841 gaattccaga aggatagtaa tcaacagaag aaataatcaa tatccaaatg aaagaagata
    84901 gctgaatcga gaaaaagagg aaaaaagaaa tgaagaaaaa aataaaatag ttactaatcc
    84961 cagagtcgtt cgtttaacat tgaaatcgat catatggata aaacagattt tttcaccaac
    85021 cctcaataga tgatggagtt gctcggatga attatttgtg tagatggcat aagggagggc
    85081 gtacagaaat gaaaaagaac aaaaatatgt tcttgacaaa aaacacatgt aatcctaaac
    85141 taaaagatgt gatgcggcta tggcggaatt ggcagacgcg ctagcttcag gcgctagtga
    85201 gggtgacctc gtggaagttc gagtcttctt agccgcataa tttgtttttt tataaagaga
    85261 aggtacacct ggagcgatga gaaagaaaat aacatattat ctaaaagaat ataggaaaaa
    85321 ttttaaacaa cggaaccaga ttcaaataaa tcaaaaaaaa tttgtgtgaa ggtgggacag
    85381 tttaaaacct aatagattga ttcaaatagt agatttatag attctgatac aagaaatact
    85441 ggcaaacgat agttaaggaa taaagactaa acttaaactc aaaaagaggt catgcgaaag
    85501 aa
//
LOCUS       DACCCM010000003        80736 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCCM010000003 DACCCM010000000
VERSION     DACCCM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.003"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            7..327
                     /locus_tag="IVK78_000165"
     CDS             7..327
                     /locus_tag="IVK78_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP6373668.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(439..1308)
                     /locus_tag="IVK78_000166"
     CDS             complement(439..1308)
                     /locus_tag="IVK78_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6373669.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(1404..2708)
                     /locus_tag="IVK78_000167"
     CDS             complement(1404..2708)
                     /locus_tag="IVK78_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6373670.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(2705..3214)
                     /locus_tag="IVK78_000168"
     CDS             complement(2705..3214)
                     /locus_tag="IVK78_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373671.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(3389..5524)
                     /locus_tag="IVK78_000169"
     CDS             complement(3389..5524)
                     /locus_tag="IVK78_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6373672.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5607..7262)
                     /locus_tag="IVK78_000170"
     CDS             complement(5607..7262)
                     /locus_tag="IVK78_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6373673.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(7264..7578)
                     /locus_tag="IVK78_000171"
     CDS             complement(7264..7578)
                     /locus_tag="IVK78_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6373674.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7748..8008
                     /locus_tag="IVK78_000172"
     CDS             7748..8008
                     /locus_tag="IVK78_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP6373675.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8022..8336
                     /locus_tag="IVK78_000173"
     CDS             8022..8336
                     /locus_tag="IVK78_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP6373676.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(9066..9620)
                     /locus_tag="IVK78_000174"
     CDS             complement(9066..9620)
                     /locus_tag="IVK78_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP6373677.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9746..11675)
                     /locus_tag="IVK78_000175"
                     /pseudo
     CDS             complement(9746..11675)
                     /locus_tag="IVK78_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(11981..12697)
                     /locus_tag="IVK78_000176"
     CDS             complement(11981..12697)
                     /locus_tag="IVK78_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6373678.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12698..14521)
                     /gene="glpO"
                     /locus_tag="IVK78_000177"
     CDS             complement(12698..14521)
                     /gene="glpO"
                     /locus_tag="IVK78_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP6373679.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14535..16031)
                     /gene="glpK"
                     /locus_tag="IVK78_000178"
     CDS             complement(14535..16031)
                     /gene="glpK"
                     /locus_tag="IVK78_000178"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP6373680.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(16168..17628)
                     /locus_tag="IVK78_000179"
     CDS             complement(16168..17628)
                     /locus_tag="IVK78_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6373681.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17618..18913)
                     /locus_tag="IVK78_000180"
     CDS             complement(17618..18913)
                     /locus_tag="IVK78_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6373682.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(19245..19922)
                     /locus_tag="IVK78_000181"
     CDS             complement(19245..19922)
                     /locus_tag="IVK78_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP6373683.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(19952..21043)
                     /locus_tag="IVK78_000182"
     CDS             complement(19952..21043)
                     /locus_tag="IVK78_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP6373684.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(21144..21578)
                     /locus_tag="IVK78_000183"
     CDS             complement(21144..21578)
                     /locus_tag="IVK78_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6373685.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(22162..23619)
                     /gene="ascB"
                     /locus_tag="IVK78_000184"
     CDS             complement(22162..23619)
                     /gene="ascB"
                     /locus_tag="IVK78_000184"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6373686.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23616..25511)
                     /locus_tag="IVK78_000185"
     CDS             complement(23616..25511)
                     /locus_tag="IVK78_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6373687.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25569..27344)
                     /locus_tag="IVK78_000186"
     CDS             complement(25569..27344)
                     /locus_tag="IVK78_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6373688.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(27394..28890)
                     /locus_tag="IVK78_000187"
     CDS             complement(27394..28890)
                     /locus_tag="IVK78_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6373689.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(29084..30706)
                     /locus_tag="IVK78_000188"
     CDS             complement(29084..30706)
                     /locus_tag="IVK78_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6373690.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(31034..32347)
                     /locus_tag="IVK78_000189"
     CDS             complement(31034..32347)
                     /locus_tag="IVK78_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6373691.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(32592..32816)
                     /locus_tag="IVK78_000190"
     CDS             complement(32592..32816)
                     /locus_tag="IVK78_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373692.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32836..33204)
                     /locus_tag="IVK78_000191"
     CDS             complement(32836..33204)
                     /locus_tag="IVK78_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6373693.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(33401..34111)
                     /locus_tag="IVK78_000192"
     CDS             complement(33401..34111)
                     /locus_tag="IVK78_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373694.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(34129..34626)
                     /locus_tag="IVK78_000193"
     CDS             complement(34129..34626)
                     /locus_tag="IVK78_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6373695.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(34663..35115)
                     /locus_tag="IVK78_000194"
     CDS             complement(34663..35115)
                     /locus_tag="IVK78_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6373696.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(35250..35855)
                     /locus_tag="IVK78_000195"
     CDS             complement(35250..35855)
                     /locus_tag="IVK78_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6373697.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(36305..36925)
                     /locus_tag="IVK78_000196"
     CDS             complement(36305..36925)
                     /locus_tag="IVK78_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6373698.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(36927..37796)
                     /locus_tag="IVK78_000197"
     CDS             complement(36927..37796)
                     /locus_tag="IVK78_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP6373699.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(37819..38226)
                     /locus_tag="IVK78_000198"
     CDS             complement(37819..38226)
                     /locus_tag="IVK78_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6373700.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(38245..39534)
                     /locus_tag="IVK78_000199"
     CDS             complement(38245..39534)
                     /locus_tag="IVK78_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6373701.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(39561..39863)
                     /locus_tag="IVK78_000200"
     CDS             complement(39561..39863)
                     /locus_tag="IVK78_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6373702.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(39888..41981)
                     /locus_tag="IVK78_000201"
     CDS             complement(39888..41981)
                     /locus_tag="IVK78_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6373703.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(42470..43234)
                     /locus_tag="IVK78_000202"
     CDS             complement(42470..43234)
                     /locus_tag="IVK78_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP6373704.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(43345..44214)
                     /gene="mdcB"
                     /locus_tag="IVK78_000203"
     CDS             complement(43345..44214)
                     /gene="mdcB"
                     /locus_tag="IVK78_000203"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP6373705.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(44239..45315)
                     /locus_tag="IVK78_000204"
     CDS             complement(44239..45315)
                     /locus_tag="IVK78_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP6373706.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(45339..45932)
                     /locus_tag="IVK78_000205"
     CDS             complement(45339..45932)
                     /locus_tag="IVK78_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP6373707.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(45936..47612)
                     /gene="mdcD"
                     /locus_tag="IVK78_000206"
     CDS             complement(45936..47612)
                     /gene="mdcD"
                     /locus_tag="IVK78_000206"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP6373708.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(47602..47901)
                     /locus_tag="IVK78_000207"
     CDS             complement(47602..47901)
                     /locus_tag="IVK78_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP6373709.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(48003..49649)
                     /gene="mdcA"
                     /locus_tag="IVK78_000208"
     CDS             complement(48003..49649)
                     /gene="mdcA"
                     /locus_tag="IVK78_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP6373710.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(49656..50567)
                     /locus_tag="IVK78_000209"
     CDS             complement(49656..50567)
                     /locus_tag="IVK78_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6373711.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            50687..51544
                     /locus_tag="IVK78_000210"
     CDS             50687..51544
                     /locus_tag="IVK78_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6373712.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(51731..52093)
                     /locus_tag="IVK78_000211"
     CDS             complement(51731..52093)
                     /locus_tag="IVK78_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373713.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            52439..53878
                     /locus_tag="IVK78_000212"
     CDS             52439..53878
                     /locus_tag="IVK78_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6373714.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(53885..56437)
                     /locus_tag="IVK78_000213"
     CDS             complement(53885..56437)
                     /locus_tag="IVK78_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP6373715.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(56551..57882)
                     /locus_tag="IVK78_000214"
     CDS             complement(56551..57882)
                     /locus_tag="IVK78_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6373716.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(57848..59326)
                     /locus_tag="IVK78_000215"
     CDS             complement(57848..59326)
                     /locus_tag="IVK78_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6373717.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(59631..61466)
                     /gene="tdc"
                     /locus_tag="IVK78_000216"
     CDS             complement(59631..61466)
                     /gene="tdc"
                     /locus_tag="IVK78_000216"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6373718.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            61936..63363
                     /locus_tag="IVK78_000217"
     CDS             61936..63363
                     /locus_tag="IVK78_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6373719.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(63455..63820)
                     /locus_tag="IVK78_000218"
     CDS             complement(63455..63820)
                     /locus_tag="IVK78_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6373720.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(63817..64182)
                     /locus_tag="IVK78_000219"
     CDS             complement(63817..64182)
                     /locus_tag="IVK78_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6373721.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(64374..64622)
                     /locus_tag="IVK78_000220"
     CDS             complement(64374..64622)
                     /locus_tag="IVK78_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373722.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            64621..64749
                     /locus_tag="IVK78_000221"
     CDS             64621..64749
                     /locus_tag="IVK78_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP6373723.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            64806..66500
                     /locus_tag="IVK78_000222"
     CDS             64806..66500
                     /locus_tag="IVK78_000222"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP6373724.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            66504..66623
                     /locus_tag="IVK78_000223"
                     /pseudo
     CDS             66504..66623
                     /locus_tag="IVK78_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            66705..66896
                     /locus_tag="IVK78_000224"
     CDS             66705..66896
                     /locus_tag="IVK78_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373725.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            67274..68407
                     /locus_tag="IVK78_000225"
     CDS             67274..68407
                     /locus_tag="IVK78_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP6373726.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            68438..68758
                     /locus_tag="IVK78_000226"
     CDS             68438..68758
                     /locus_tag="IVK78_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373727.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(68787..69251)
                     /locus_tag="IVK78_000227"
     CDS             complement(68787..69251)
                     /locus_tag="IVK78_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6373728.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(69383..70637)
                     /locus_tag="IVK78_000228"
                     /pseudo
     CDS             complement(69383..70637)
                     /locus_tag="IVK78_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(70666..70983)
                     /locus_tag="IVK78_000229"
     CDS             complement(70666..70983)
                     /locus_tag="IVK78_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6373729.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(71009..71329)
                     /locus_tag="IVK78_000230"
     CDS             complement(71009..71329)
                     /locus_tag="IVK78_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6373730.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(71793..73208)
                     /locus_tag="IVK78_000231"
     CDS             complement(71793..73208)
                     /locus_tag="IVK78_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6373731.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(73225..74541)
                     /locus_tag="IVK78_000232"
     CDS             complement(73225..74541)
                     /locus_tag="IVK78_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6373732.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(74691..75389)
                     /locus_tag="IVK78_000233"
     CDS             complement(74691..75389)
                     /locus_tag="IVK78_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP6373733.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(75678..76667)
                     /locus_tag="IVK78_000234"
     CDS             complement(75678..76667)
                     /locus_tag="IVK78_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP6373734.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(76747..77580)
                     /gene="msrA"
                     /locus_tag="IVK78_000235"
     CDS             complement(76747..77580)
                     /gene="msrA"
                     /locus_tag="IVK78_000235"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6373735.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(77755..79383)
                     /locus_tag="IVK78_000236"
     CDS             complement(77755..79383)
                     /locus_tag="IVK78_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373736.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(79370..80704)
                     /locus_tag="IVK78_000237"
     CDS             complement(79370..80704)
                     /locus_tag="IVK78_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373737.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        23717 a        16924 c        13614 g        26481 t
ORIGIN      
        1 gatcgaatga ttacaacaac aacgaacagt gtagagggaa aagccgtagt agcttataaa
       61 gggatcgttt ttggtgaggt cattactggg atccatgcat ttaaagactt ggaggcgggg
      121 ttgcgaaata tctttggcgg ccgctcaaaa agctatgaaa acgaactaat gggtgcaaga
      181 gaagaagcac ttgccgaaat ggccgaacgt gcaaaagcat taggagctga tgcgatcatt
      241 ggcgtaaaaa tggactatga agtattaggc agcgacaatg gcatgctgat ggtcacatgt
      301 agtggaacag ctgtctccac gagataagta ggggacatct gttttaataa ctaaagagag
      361 atgggacatg tagtggcact caagaattag atttttcagt ctaacttcga ggtactctac
      421 cttcccatcc tctctttttt atttcttttt gcggatgatt ccgtaagctg ctgcgactaa
      481 cacacccgcc cctgctaagg aataactgac agaagctgtt ttttcatttg tttttggcaa
      541 agtctgactt gttgatttgg atggctctgt cgtttgctga actgtttctg tcgtgcttcc
      601 agaggtcact gatttttctc ctgccgaatg caaaatatct tttattcgtt ggaaactagc
      661 tgctaagtca gctttttttg cttcaaaatc attagcaact gtttgtgctt cgctgatctt
      721 tccgtggatt gcttgactgg ccgctgtgat atccgcttga gtttgactta gctttccaga
      781 agctgtttga gcaacctgat ttaattgctg attgaattct tttgcttgtt ggatcttccc
      841 gtcaagatct gtttggacag cttccgcttt tgctttcgct tcggcaagct ttttctcaag
      901 ttgtgcacgg tcatttccca ctttattttt aagatcagct aaatcttgat cgattgctgc
      961 ttggtcttct ttcaatttgt tttttgcagc ttctaatgct tcttcgagtt cttttctttt
     1021 attttccaag tcttgcaaat gatcgatttg gtcagaagag gtagaactat ttattgatgt
     1081 atcttctgaa gatgaaatcg tttctcctga attatctgtc ggtacattgg aagtcatttc
     1141 tgtttccgag gcaccgtttt cagcaccaga agagcttatg gttgttgaag gttctgtttg
     1201 agcaggttca gaagtttccg caaaagctgt ctgtcccaat aagaaaatac tggaaacagc
     1261 aattggccag attaacggct tcattctttt tttcatcact tttcccatcc ttactacttt
     1321 ttaactaatt tcttattata agcaaaaatg tttggaagaa ccataaacac aacaggctgt
     1381 ttgtctaaaa aacggtgcat cttttatttt tttgtacgtt ccgggctttt ccatgtcccg
     1441 cttccataac ctaaaatcgt tttgactgct ggaatgaaag tcgtcacgat cgttatcgcg
     1501 ttcactatcc agaacaaaag catatatagt ggtgcgaaaa gaaggtattt caattttctt
     1561 cgtcgatcat ctacaatcag cgaagctagc aactggaaaa atcctgcgac catttcaaag
     1621 cagatgaaca caaaactgaa tgtaaaagta atataaatct gttcgtagtt tccttgatat
     1681 acataatgcc cgattaatcc agcaaaaaga actacggaaa tacaaaagaa tattgaccag
     1741 acaatactca atgtttgatc aatgaagatg atcgttcttc caatatgttt aaaaggatgg
     1801 agcatcactt ttttgaaatt cgtcagccag acttctgttc caccttttgc ccaacgtttt
     1861 ctgtgtcgat atagcatctt caacgtcaca ggtacttcca tgaaaaagat aatctcagga
     1921 gcaaaaacag ataaccagtc atttaactga tgatcccaag caatactgat gtcttctgtc
     1981 gctcgatctt gtctgaatcc gcccacatcg atcaaggctt cttttcgata catcgtattt
     2041 gctccactgt acgcataaag accaccgaaa acagctgact gtgttcgttt aatgatgccc
     2101 acgatacttg agaattcaac cgtttgagat tttgcaatca atttggtacg attttggaca
     2161 tccatatttg cagtgaccgc agcaatatgt cttgcaccag ggcgaatgaa ataattcaca
     2221 tacctaatca gtgcatctgg ctccggtacc gtgtctgcat cattgcttaa aatcaatttc
     2281 ccttttgcaa aggcaagtcc gatattaaac gcgtgggctt ttcctttatt tttttcgatc
     2341 cgaaccacac gaagattagc gtattttttc atcagtctag ctaatatttc tggtgtttgg
     2401 tccgttgaac catcgtctgt cactaatacc tcatagttgt gataattcaa tttcgtcatc
     2461 aaatactcga ttgtatattc gatcacaatt tcttcattat gggctggcac catgatcgtg
     2521 atgaacggtt cgacactcaa gggaacatct acccattctt tttgcttatg tttgtataaa
     2581 aacacataac accagacacc aataaaccat acaaatccgc ctaaaatcgg ataagaaacg
     2641 agaaaaaaat acataataga caaacaagta tctaagaata aattacctgt atgggtgatt
     2701 gagatcattt cagttccacc ccattttctt tatacaaatc tgcaatgaat gtcgtatcta
     2761 gatttttctc ttcggagaca gaataaaaac ggaccgtttc tctctcttct tttgttccaa
     2821 aacgttccgt atacacttct tcaagtaatt cttgtctttt tttcaatttt tcatcttcat
     2881 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
     2941 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
     3001 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
     3061 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
     3121 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
     3181 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
     3241 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
     3301 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
     3361 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
     3421 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
     3481 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
     3541 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
     3601 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
     3661 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
     3721 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
     3781 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
     3841 gatggtagtc gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
     3901 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
     3961 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
     4021 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
     4081 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
     4141 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
     4201 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
     4261 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
     4321 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
     4381 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
     4441 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
     4501 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
     4561 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
     4621 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
     4681 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
     4741 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
     4801 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
     4861 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
     4921 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
     4981 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
     5041 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
     5101 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
     5161 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
     5221 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
     5281 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
     5341 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
     5401 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
     5461 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
     5521 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
     5581 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
     5641 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
     5701 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
     5761 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
     5821 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt
     5881 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
     5941 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
     6001 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
     6061 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
     6121 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
     6181 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
     6241 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
     6301 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
     6361 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
     6421 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
     6481 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
     6541 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
     6601 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
     6661 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
     6721 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
     6781 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
     6841 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
     6901 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
     6961 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
     7021 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
     7081 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
     7141 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
     7201 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
     7261 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
     7321 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
     7381 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
     7441 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
     7501 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
     7561 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
     7621 atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt ttttcactac
     7681 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
     7741 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
     7801 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
     7861 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
     7921 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
     7981 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
     8041 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
     8101 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
     8161 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
     8221 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
     8281 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
     8341 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
     8401 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga
     8461 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
     8521 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
     8581 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
     8641 gtcagctgat tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta
     8701 ttaagaaaaa ttttgtaaaa atggatttct acatttttgc ataaatttgg cttaataccg
     8761 agaaactggc ttttgaatac cgtttaagga agaggttgta aaatcagcgg tttgacctga
     8821 gtattagaga aacaaacgga aaaatagctg ctcatatttt gccgtttttt gagctaatac
     8881 cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca tttaactccg
     8941 aatcacaaga aataatttca aaaacaactt ctcctgtttt tggttcggtt ggcttattat
     9001 cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata ggtcacaccc
     9061 cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac aaaagattgt
     9121 aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg taattctggg
     9181 aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt gccataccac
     9241 ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac aaacataaag
     9301 ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata ccatgccata
     9361 gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac cttccctaag
     9421 tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga caaacctgag
     9481 acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata aagataaatc
     9541 ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat ttctttacgc
     9601 cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt ctttttattt
     9661 tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt tacagacact
     9721 gatattatga gctaatttga gataatcact tgctgttttc atcgtttact cttctttctt
     9781 cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat cctcgctcta
     9841 ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa tcgtaacctg
     9901 tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc acgtggatta
     9961 tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa actaacgctt
    10021 ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctga cgttcttttt
    10081 tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc tgtgtccgat
    10141 aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac atatccattt
    10201 tatgagcaat ccacaaagct tggctttctc ctgtttcttc caacccgcaa ctgatattta
    10261 ggacgaagtg cttcccgatc gaaagccata gcagccggca caaaatcttc atgatctccg
    10321 gcgaatttct tgatttctcc ataatgggtc gtggcaatga tcaatgcccc ttgctcatac
    10381 atagattcca tgattgcgat cgctagtgca gccccttcat ttggctctgt tccactgcca
    10441 atctcatcta aaagtaccaa cgtattcctt tttatctttt tcaaaatagc agcgatgttt
    10501 tgcatatgac cagaaaatgt actcaacgca ttttctaaat tttggtggtc accgacatcg
    10561 acgaaaatct catcaaagac agcaagctct gtcccttctt ttgccggaac ttgcataccg
    10621 aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt gcctccggca
    10681 tttgctccgg tgataatcaa gcctcgatag tctttaccga tttcaacatc caatggtacc
    10741 gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc cgatttattg
    10801 attctaggtg tgcttccatt gatttctcgg ctaaattttg cccgagcaaa gatgatatct
    10861 aacaccgtga tcgtttcgat caataaatcg atcacttctt cgttttctgc gatagcacca
    10921 gtcaatgccg ccaggacttg atattcttca ctgatttctt cagcttttaa caactgatag
    10981 tgttcattca atttagaaat cacagttggt tcaataaaaa ccgtcgttct tttatttgat
    11041 tcatcgataa tcgttccatc tattttattt ttatagctgg ctttgatagg aatcgtatag
    11101 tattcgcctt tttgaacaat cattgcttct tggatcattt ctttgttttt cggatgacgc
    11161 aagaatttca gtaattttga ctggatttct ttttcgatta gttgtaattg tttgcgtact
    11221 ttacgcaaat tacgtgacgc gtcgtcactg actttattat tttttatttt ctggtcaatc
    11281 aactcttcta catttatcaa atcaggaaga tgtttgctat aagcaaacaa caagggtgtc
    11341 tggtattgat tcttatcaaa aaactttgtg atcatccgac tgcttctaag aaaatccgca
    11401 tattcgatca aatcggttgg ctgcagaatc aacccttttt tgacttgttc tgtcagtgca
    11461 tcgatcctcg gcaaccccat aaatggaaca tgctgactgc tttctaagat caagcgtgct
    11521 tctcttgttt cttcttgacg atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg
    11581 ctgattcgtt ttttgctata gtttccaatt gctcttgctt ggatttcctt tttgatccga
    11641 tcaaattgta attgactaat catttcgttg tccatattct ctccttttct tgaagagata
    11701 cctgtcccaa aataaataag ggcctagaga taaataaaag gatagaacac agcttaaaaa
    11761 agtcatgtcc tatccttatt agaatacaaa gatattgttt tcctgacagg taccgtttca
    11821 taagcacacc aaataaactt tgtttattgg ctattcgact tactgattag ataccagcta
    11881 cactcacaaa acatacccca ttcttctagg tcttatttat tttccactta actatagcaa
    11941 gacacatttt ggttgtcaaa ctttagtatg ttttatagat ttacaccatt aatgaataga
    12001 cacctaccgc gataattgct ccgatcattg gacctgcgat tggtacccat gaatatgccc
    12061 aatctgaagt tcctttatta gctattggca ataccgcatg tgctaatcga ggaccaaagt
    12121 cccgagcagg gttgattgca tatcctgttg gccctcctaa tgaaagaccg atggctaaga
    12181 tcaatccacc tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta
    12241 gcaacccaag aactaataca aatgtaccaa ttaattctgt gatcacattt gctggataat
    12301 tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat
    12361 tccaatgtgg caaataagaa agccaaacaa gaatggcccc tgcaaaagct cctaaaactt
    12421 gcgcaatgat gaaaggaacg acaagattcc aactaattgc ccctgtcatc gccatagcta
    12481 gagagactgc aggattcaaa tgagctgggc ccatgaatcc agaaatatag acagccattg
    12541 ttacagcgag tccccaacca aatgcgatga caatccagcc agaagcctgt gctttacttt
    12601 tgtttaaatt cacagccgca cacacaccat ctccaagcaa aactaggatc agtgtcccaa
    12661 taaactcccc catgatttga gtcatatcac tgttcattta tttctccccc tttagttccc
    12721 gcagatctga ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt
    12781 ctgtccattc aaaatatcca gcaattgcat cgatcacagg ttgcttgatc tcatcaagct
    12841 gatctcgttc aaacaacaga tgatttgtac gacgaatgag ataatcgcct ggggccaaaa
    12901 ccatttcttc ttctaaacca taacgtaacc gagctgactc agcgagactc aaaccaggat
    12961 aaggtgccat ttcttttgct aattcaaaga tacgtcgggc attagtcccg taaaaatcag
    13021 cgatataagt agcatcttct tcctctaaac cggctgctac tccttctttt gctaattctg
    13081 tcactgtttc ttctaattta gttggatcga agtttccacc tgaaatctga tattttttag
    13141 agtcgatttc tttcgtctct attccgtatt cttcttttaa taattggcgt atcaatcgca
    13201 aagctccttc tgccatctta cggtaatcgg tgattttccc accagataaa gtcagcaaac
    13261 catctggttc tctttctaaa gaactacctc tggaaatcgt agaaggtgct ttttcatcac
    13321 ggctgttttc caaatggttc aacacgtctt ctacttcagc acgagaaact ttgttttcct
    13381 tatattcact tactgtatcg acaactttat tgaagctctt gtctgaaatc gatccattat
    13441 ctccaccatt gtaatcagaa ccagaattac caatcaacag tggacgaagt cctgcccagc
    13501 ttgcttcgat atcattcaat gtgatatttg cttctggata gcgatgattg atcacatcca
    13561 atagataatc tacatcttct tgtgtgactt tcggatccgt aaaatctcct tggtaatccg
    13621 tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc atccgcccat
    13681 cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg cagttttttc gcatctacaa
    13741 ctaaatggat ccctttagtt ggacgcattt taggagagac tggacgcgta aaattcaagt
    13801 ttcgtacttt atctacccaa ggaccactcg tattgatcac taattttgct ttgatttcga
    13861 tcacttcatc tgtcagcagg tcacgagctt tgacgccaac gatttgatcg ccatcatata
    13921 aaaagccaac cgctttcatt ttacttacta gataagcccc atcttcggca gcctttttga
    13981 tattatcaat cactaaacgg gcatcattgt tgcggaaatc tagatagaca cccgcgcctt
    14041 ttagcccttc ttttttcaaa aacggttctc tttccaatac ttcttctggt gtcagcgtat
    14101 aattctcata tttggttcct gtcacatttg ccagtttgtc ataaaggtcc attgctactt
    14161 tgaccgagaa catattgaag gttgttgctc cttcgccctc atagattggc aaaagcattg
    14221 gatctggttt tgggatgtgt ggggcaatac tttgtacgac tgcacgttca ccaactgtgt
    14281 cagctactac ttccacatca aatgtcttca gataacgaat accgccatga acaagtttgg
    14341 tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc gatcaatcct gttttgattc
    14401 ctgatgctgc tgactggact gccacaccgg caccagtgat tccgccaccg atgatcaaca
    14461 gatccaattc ttcttgctgc attttttgaa tgctatcttg tcttgtcttg tttgaaaaca
    14521 tatggatttc ctccttattc aaatgcttgg gtagctttta ctgctttttt ccaaccgttg
    14581 taaagttttt ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc agcttcataa
    14641 aattcgcgga tctcgtctgt atctttccaa taaccgacag ctaatcctgc taaaaatgct
    14701 gcccctagcg ccgttgtttc caagttttct gctcgttgga tcggtacatt caagatatct
    14761 gtttggaatt gcatcagata ttcattgttt gctgcaccgc catctacttt caatacagga
    14821 atcttgattc cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg ataagcaatc
    14881 gactgtaaag tagctttaat gatatcttct tttgtcgtac cacgtgtcaa gccaaacata
    14941 gcccctcgtg ctgcctgatc ccaataagga gcacctagcc caacgaatgc aggaacaaca
    15001 taaacctcat cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct agctttttgc
    15061 aacatttgaa gtccatctct cagccattga atcgaagaac cagccacaaa aatacttcct
    15121 tctaaagcat agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt
    15181 ttggataatt gcggtttttc accggtattc atgacaataa atgaacctgt accataagta
    15241 ttcttgacca tgcctggttc gaatgccatt tgtccgaata atgcggcttg ctggtctcct
    15301 gccatccctg aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt ataaccataa
    15361 acttctgagt tagaagtgac ttttggtaac atcactcgag gaatatttag taaatctaaa
    15421 atctcctgat cccaatctag ctgatggata ttgaacaaca tcgtccgact ggcattcgag
    15481 taatcagtaa catgtgcttg tccatcggtc agtttccata ctaaccaaga atcgatcgtt
    15541 ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc
    15601 cagcgtatct tcgttgctga gaaataggca tcgatcacta accctgtttt ttcgtggaaa
    15661 aattcttgat agccttcatt ttttaagtta tctgcaattc cagctgactg tctggattgc
    15721 caaacaactg catgatagat tggacgtcct gtatcttttt cccatattac tgttgtttca
    15781 cgctggttcg ttatgccaat cccagcaatc tgatttggtt tgatccctga ttcgatgaac
    15841 gctccggcaa taactgactg tacagaattc cagatttcat ttgcatcatg ttccacccaa
    15901 ctttcttgcg ggaaatattg cgtgaactct ttttgagaac tgccgatgtg tcttcctttc
    15961 ttgtcaaaga taattgctcg tgagctagtt gtaccttggt caatcgccat gatatacgtt
    16021 gattctgtca ttgtgattcc ctccattata tgcaaacgct ttctttaact actcttatca
    16081 tactactgtt ttaaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca
    16141 gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg tctctattct
    16201 tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg
    16261 ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact
    16321 tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat
    16381 acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa
    16441 atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc
    16501 agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt
    16561 taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc
    16621 gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc
    16681 cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat
    16741 gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa
    16801 tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta
    16861 tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg
    16921 ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt
    16981 ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga
    17041 tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg
    17101 tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata
    17161 aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa
    17221 ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt
    17281 aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat
    17341 cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc
    17401 gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat
    17461 tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac
    17521 atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat
    17581 caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg
    17641 ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt
    17701 cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt
    17761 ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata
    17821 gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg
    17881 tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga
    17941 aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc
    18001 cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat
    18061 acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt
    18121 ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta
    18181 attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa acaagatcat
    18241 agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa
    18301 cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg
    18361 tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt
    18421 gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa
    18481 ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt
    18541 taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag
    18601 tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt
    18661 ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt
    18721 cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat
    18781 tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg
    18841 ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa
    18901 ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc
    18961 agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat
    19021 caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta
    19081 attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa
    19141 actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt
    19201 tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct
    19261 tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc
    19321 ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct
    19381 ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct
    19441 tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta
    19501 actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag
    19561 atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg
    19621 ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta
    19681 tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc
    19741 atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct
    19801 gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa
    19861 cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc
    19921 attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat
    19981 ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt
    20041 catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc
    20101 caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc
    20161 caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg
    20221 atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca
    20281 gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga
    20341 tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag
    20401 catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat
    20461 tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt
    20521 ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc
    20581 tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc
    20641 gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc
    20701 cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg
    20761 cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag
    20821 cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa
    20881 taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca
    20941 ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt
    21001 gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa
    21061 ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat
    21121 gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt
    21181 gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt
    21241 tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact
    21301 aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca
    21361 aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt
    21421 ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt
    21481 ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa
    21541 agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac
    21601 atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg
    21661 tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa
    21721 ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa
    21781 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa
    21841 actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa
    21901 taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat
    21961 ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc
    22021 acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg
    22081 aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc
    22141 acagtccctt atttcctttt attataggat ttcgccattt gattggatga cttgtctgta
    22201 ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc
    22261 aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat
    22321 gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg
    22381 ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc
    22441 tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc
    22501 atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact
    22561 tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga ccccttccca
    22621 attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac
    22681 agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc
    22741 ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa
    22801 caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg
    22861 aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca
    22921 agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg
    22981 ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac
    23041 acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac
    23101 tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc
    23161 tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct caatcccata
    23221 ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc
    23281 ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc
    23341 catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct
    23401 tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat
    23461 catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc
    23521 agacacagat aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt
    23581 cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct
    23641 agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta
    23701 tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct
    23761 acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag
    23821 tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct
    23881 tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca
    23941 gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta
    24001 gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt
    24061 aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt
    24121 agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca
    24181 ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct
    24241 gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt
    24301 ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata
    24361 aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt
    24421 actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg
    24481 tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact
    24541 aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca
    24601 gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat
    24661 aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt
    24721 tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt
    24781 gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag
    24841 gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc
    24901 atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc
    24961 aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta
    25021 gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc
    25081 aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg
    25141 ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg
    25201 gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca
    25261 gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta
    25321 ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct
    25381 tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc
    25441 cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac
    25501 ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta
    25561 tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc
    25621 ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt
    25681 atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac
    25741 aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat tagttgctga
    25801 atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc
    25861 agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc
    25921 tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa
    25981 ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt
    26041 ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat actttgttga
    26101 atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc
    26161 ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca
    26221 gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct
    26281 aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc
    26341 atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac
    26401 gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct
    26461 atttctttgt ttttttcatc attaatcatc aaataattta actctaatag tcgattttca
    26521 attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg
    26581 agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct
    26641 cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca
    26701 atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg
    26761 gaaaaaattg tactgatttg ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca
    26821 acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc
    26881 gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt
    26941 tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca
    27001 tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa
    27061 tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat
    27121 tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca
    27181 agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat
    27241 tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt
    27301 ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc
    27361 aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag
    27421 gtttttacca ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct
    27481 cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct
    27541 catttcccct gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat
    27601 ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca
    27661 acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt
    27721 ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa
    27781 ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt
    27841 atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc
    27901 gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc
    27961 tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg
    28021 ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga
    28081 accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca
    28141 cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt
    28201 cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg
    28261 ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa
    28321 agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa
    28381 gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata
    28441 ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc
    28501 atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt
    28561 ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa
    28621 acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc
    28681 atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt
    28741 ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa
    28801 tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa
    28861 aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct
    28921 tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga
    28981 agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac
    29041 gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca
    29101 caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat
    29161 ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca
    29221 tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct
    29281 caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca
    29341 aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg
    29401 cattgatttc tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag
    29461 catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga
    29521 ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga
    29581 ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact
    29641 cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct
    29701 tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat
    29761 cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg
    29821 ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt
    29881 cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag
    29941 gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca
    30001 gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc
    30061 cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa
    30121 agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct
    30181 gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc
    30241 ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct
    30301 cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt
    30361 tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga
    30421 tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac
    30481 cgaattctgc catgattgct tgataattac tgatatcata cccattgtca tcattcggag
    30541 actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc
    30601 tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg
    30661 gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat
    30721 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
    30781 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
    30841 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat
    30901 ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac
    30961 ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga
    31021 tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca
    31081 gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat
    31141 agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca
    31201 agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt
    31261 gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg
    31321 atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga
    31381 tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag
    31441 aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta
    31501 cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc
    31561 ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag
    31621 aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg
    31681 cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag
    31741 ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga
    31801 ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg
    31861 tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac
    31921 cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc
    31981 gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg
    32041 ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc
    32101 caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa acacagccga
    32161 tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta
    32221 atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca
    32281 gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt
    32341 ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat
    32401 ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat
    32461 tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca
    32521 tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct
    32581 agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg
    32641 aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt
    32701 tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca
    32761 ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt
    32821 tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc
    32881 agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc
    32941 catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc
    33001 taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc
    33061 ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat
    33121 ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag
    33181 catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg
    33241 tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca
    33301 agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa
    33361 aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct gcttctgatt
    33421 catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg attttttcct
    33481 tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg gattcaatga
    33541 ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca cgccagttga
    33601 gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga agtgccggag
    33661 catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt cctaaatgca
    33721 gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa gacaaggaaa
    33781 tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct tgatttgcac
    33841 gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat gtggtaaatg
    33901 cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta aggtaatttc
    33961 ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt tcttttgtta
    34021 cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga aatacttccc
    34081 agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt agaataattg
    34141 cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat gttcgctgta
    34201 tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt cccaaacacc
    34261 gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat ctagtaaaaa
    34321 tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga tttctaaaaa
    34381 atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt aacccgcagg
    34441 ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct cttctgtaag
    34501 tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg aattatcttt
    34561 tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg
    34621 cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt
    34681 gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata
    34741 ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt
    34801 gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat agagtttctt
    34861 gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt ctaatttctt
    34921 gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt tttcttggat
    34981 gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg ttaaatcgac
    35041 ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc cgatgtctcg
    35101 aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt cacctctttt
    35161 tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata actagtaaag
    35221 tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt tttgttttct
    35281 aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa ctcttctata
    35341 ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc ttcaccgtgg
    35401 atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc tttttgttta
    35461 ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt ttcttcatag
    35521 acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc accaggaagt
    35581 gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt tttgatatag
    35641 gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag cgcttttatt
    35701 tcttcgaaag tttcttttcc tatttcactg attagttcca atgaaatcgt ccggatctcc
    35761 tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg agaaagtgcg
    35821 agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt tttagcaaat
    35881 gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa atataatgaa
    35941 atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc gacttctctg
    36001 gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc caccaaacga
    36061 aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg acagcttttt
    36121 cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag tcacaatatt
    36181 catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag ctgaacgaca
    36241 ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt aagccatttc
    36301 tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga gtgtaatcca
    36361 cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat ttgtcatatt
    36421 ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc cctttcgttc
    36481 gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa tgacgatctg
    36541 attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct gttaggcgaa
    36601 tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct ttttgcttat
    36661 cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac aaatcaagtg
    36721 ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct tccaaattgt
    36781 taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg gcatacatat
    36841 ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca ggactaccag
    36901 ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg actttccaag
    36961 ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt tgctggaatc
    37021 acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt agctactttc
    37081 gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt attgatcaat
    37141 tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg gacatcaact
    37201 actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt gaattgtcca
    37261 tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac aaatttcact
    37321 gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc ttcttctgta
    37381 ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc atattctact
    37441 actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc atacatgaca
    37501 gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc gtggtctaag
    37561 tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt gactaacaca
    37621 tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg cgaacccatt
    37681 tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt aaaagctcca
    37741 actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt atacatcttt
    37801 tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg caatttgtcg
    37861 catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag cacgagcagc
    37921 atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc cgatcgtctg
    37981 tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat acaattcaga
    38041 aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt gggtagtgga
    38101 aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt ctggcgatag
    38161 ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct taaaaaatcc
    38221 aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta agatccaggc
    38281 atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattga tgaatcccat
    38341 cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac catgagcaat
    38401 accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc
    38461 tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga acatgggcaa
    38521 agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt
    38581 tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag gaactaattt
    38641 catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta caccagctag
    38701 caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt
    38761 ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag tgatgatata
    38821 tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt
    38881 cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa aaagcttagc
    38941 aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa tatcatttga
    39001 tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg cgaaacaagc
    39061 tccgataaaa tcaccgacca agatcaatac gatccctcgc agaccggcaa aatagccaaa
    39121 gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa tgtacttcca
    39181 ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg tcagtgcaat
    39241 atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa tagaagggat
    39301 gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta aacttccaac
    39361 gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta acgtgccaga
    39421 gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa tcaatgcaat
    39481 ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc ccatgttttt
    39541 accctctttt ctttttgtat ttagataact cccttatctt gcagaactgg ggtgattttt
    39601 tcttcaattt cattttttga aacaatattt ttcaaataga tcatcgccgt tttaggatca
    39661 actgcgaact tttcgaactg cgaacgaaaa ttttctgctg tgatgatcac atctggtttc
    39721 atggaaactg cggaagaaat atcacaatgg tccagtttgg cttctactcc taatttgttc
    39781 aaaacatcct cagcagacat ctgtgctgca aaactacttc ctaaacctgc accgcatacg
    39841 aataaaatgt tcaatttgtt catctttgtt ctcccccgta tcatttatta tttctcaact
    39901 actgaaaaaa gcagttgttt gaaatcttcg atatttgtgc attcaatcaa tcgatccaac
    39961 ttctcttcgt tgttgatcaa ttcaattagt tctttcatga tgttcagatg cgagtaggag
    40021 tctactgctg ccaaacaaaa aataatcttt actggatcca tttcaggatt accgaaatcg
    40081 attggttgct caatcgtagc tacgctgact cctaatttat tgaccccatc ttctggtcgg
    40141 gcatgggcta gtgccatgtg tttgccaatc acgatatacg gtccgtaatc attcactgca
    40201 cgaatcatcg cttcgacata gcgctcttcg acaatgtttt ctttaaccaa tggtctagca
    40261 gctagttcaa tcgattcctc ccaagtagtg gctttttctt tgatcatgat attgctgtcc
    40321 gtcaaaatat cttttaacat aggctggatc tcccttttgt tgatttctaa attgtttgtt
    40381 tcgaataatt tttctaactt gccatagatg attcgatcta ctcggccacc actttctttc
    40441 atgacatcta ttatttgagt gaataactcc gtcgattcat gactgttagg ctctaaccgc
    40501 tgtagttctt catttctttt caaaaagtct tctataacca agatattcgt ctcagttaaa
    40561 atcggatcga caactaaaag cggcttattt cgataattca attgaaaggt gctgaaaacc
    40621 agattgacat ccaatttatc tactaattcg atttcttttg aacttaaaac tgcagtaatc
    40681 tcgatctgag gaaacttttc tttcagattc tctgctaaca gatttccagt agccatccca
    40741 tggttacaga caacgactga tttataaaca taattcaagt ctcgcttgat tacactggcg
    40801 atcgtcgaaa agtggatgac aagaaaagct atctcgtcct ccagtatctt gccaccaatt
    40861 acttcttcga tcgtaggaac aaaatgagtg actgcgcggt agattgggaa ataatttgtt
    40921 cggatgtttt ctttcaatgg attcatgact tggatatgat tcttgattct tacaatcagt
    40981 gcagccatgt gcttatagag attttcgaat aaagcttctt cctttagatg gaaaggtacg
    41041 tgtgtctcgt tctctacaaa ttggatcaat tggatcgtta gtaattgagc ttgtacccac
    41101 tcgattgaat tggagatatc tctagcattg aacgtctcaa tagtgaaacg aagataattt
    41161 ctttctactt gcgggacatt gtccaattga aattcctcga tcatgtggtc aataaatgca
    41221 gaaaagctac tgtcttttat ttctgtattt ttccaattaa tggcagagat aaattcatgt
    41281 ttcctgattc gctggatcca gatcaatgta aaaagcagga tttgtttttt ataaatatca
    41341 tcttcttgtt tcaacaactt attggaatag atctcctgta tttttttgat ttccaccaaa
    41401 ggaaaataat ggagaaagat tttttgagcc ggtgttaatt gtgcatcttg attcataaag
    41461 aagtcggtca ttcccagatt ctgattgata aaatcataga tcatcgtacg gatagatcgt
    41521 tctgaacctt tataaaccag tccttgcttt ccataactga cgatctctat cccataactg
    41581 accagtgttg ctcgtaaccg tctcatatct tcatctaatg tacttttaga gatttgaaat
    41641 tcttcttctt ttttgtttag atggatcggg attcggctgt aagcaacggc taaaactaga
    41701 tcaaaaatac gttcgcgccg attcaaaaag ccgtctttca catcttttat ttcagtttct
    41761 aatcgagctt gcttttcctt ttccagcttc aacaaaaaac ctttgttgcg aattgtacgg
    41821 atctccggaa gctttttact gctcaaccag atattcgctt caagaacttc ctgacggatc
    41881 gtccgctgac tgacattgaa ctcacttgct agatgttttg cttttactgg atattgggct
    41941 gtcatgattc tgtgaagtat ttcaattgtt cttgttttca tagaaatgat ctccttctga
    42001 tttcaagtat atggttccgc ttacataata aaaagtagat atttctgcca cttgaagtgg
    42061 aaagaaagta agcaaagcaa acaaaaaata agccatatgc tttttcatct cctcaacgaa
    42121 aaagacatac gcctattatt acaaaaattt tgcccatcta tacaaaaaag aggttgtgaa
    42181 aaagctgtcc tgcatcaagt aataagaaca actaaactaa aatagtgctt cctatttttc
    42241 gtttggttgt tcttattatc gcagatgcaa gcttttaaac accatttagt ggagaggttg
    42301 tgacagagat gttcagcctt gagcgataag aagcgatttt gaaaaatcgc ttcccatatt
    42361 ttacaaaatc gtgccttatt gccgataggc tatctctggc acaccgttta ttcagaaaaa
    42421 ggagctgcga caaggctttt gtcacaactc cgttcataaa tatttcctgt caatccagca
    42481 taatcggctg aataaaatcg gcttgccatc ttttttcttt ctcagaaaaa tcaaaatacc
    42541 atatctgaga aaaatactgt cgttgtgtct tacttcctga gatcaatcgc ccatcttttc
    42601 gatcgactga ataatcacag catctgaaat caattcggat ggcaaaacta ttttcgtgga
    42661 tctgtcgata atttcctact tcctgaatag ttattttttt agtatgattt cttacaccgt
    42721 ctgctttgtt tttttgcaga actttctgat gttcggcaaa cagcttgtct gtataacagc
    42781 ttctggcttt ttcaggagtt tcttcttccc aagcggattg gatcttcatg aatatctttt
    42841 ggatttccag aaactgtgct ttcttttctt tagccgttcc tgacatgcgg gacaatatct
    42901 ccgctttcag ccttttcttt tgtcttttat tcttgacgat agaaaaagca gaaacccctc
    42961 ctactgacaa aacagctaat gcaagaaatc ccgtttgctc ataactagaa ggacctgtac
    43021 ctggaccata atacccataa gaatctctgc cgtaagaacg actgccaaaa taactgcccc
    43081 catatgaacg accaccaaat gaacctctgc ccgaacttcg accgctagat gtacctccac
    43141 catgaccacc cgcgacagcg aatccttggg tcggcagagc aaacaaacat aggcccacta
    43201 acaaaatgat tgctaaataa ttgatttttt tcattgtctt cctcgttatc ttttattaca
    43261 aaaaactatt tttatgatag ccttgctaaa aggaagagtc aataaccaga tgcataaact
    43321 gtttttttag caattttgtc ttatttagtc attgagtaac atcggacaaa tgtatgtgtc
    43381 ggcagtccat aattcctcta tcttcagcat gaacaagctt gcagacaaga gatctgcgct
    43441 tcctccagga gagatttggt tttcctgaca aaagtgcaac agtgctgtaa atggacgatt
    43501 tggtaaagca ctttgactag cagcatgagc aagtttttgc atttccacta acgttttctg
    43561 atcactgcga tgcaatacgc atgtgtcatc tagcgaactc atcaaaacta acaacatatg
    43621 catttgtttc tgtacatatg aatcattatc tagctcctta gctgcttgaa ttgcaagcct
    43681 gatatgcgga taccctaata tcgcttcttc atatgcacca tttacgccat atttttgctt
    43741 caccctatga ccatgagtgg tttttttatg acgatatttt gtatcaggaa aagcagcaag
    43801 cttgctgaca tcatcaagta ttttcgataa agaaaaaggc tgttccatac tgattttttg
    43861 tgcaatgaca ctgatcaata gccccattgc ccaaatcgct cctttatggg tattgatgcc
    43921 atttgtagca atcagcattg ccttttccgc ttttcgccca atctcagcaa ttctttctct
    43981 taactcctga tcaatagcat gatgccatgc tatttgagcc atttcttcaa aaaaaggagt
    44041 tagcgtctta gcagacttaa gaaataaagc atagtccata tcggaatgtg cacccgcatc
    44101 cagtgcatcc actaatccag gttttggact taatgttact tctgcgatca atgcttcctc
    44161 taccaacgca gcgagttgct gagtcactgc tttttttcgt tcgtcattta tcatgacact
    44221 cactccttct tcctactatt acttgctgaa accttttttg atagctaccc aaatgatatg
    44281 aagtaaccag cttaaagcaa atgcacaact tgccgtcatt agataagcta caagaatcat
    44341 gactcctgaa ggtaactgca gccaactatc tttaaacaat aataacagta cttggaaaac
    44401 aaatacattg gctaaatacg tacgataagc ataaacagac aaccatttga acaatggcaa
    44461 ttttggcgaa tcgtttacaa tcattttgga agcgcctatg aagacagcaa agatgatgac
    44521 taaactgtat aaactttgga tcgtattcaa gtaaggtgca ttcgcaaagc tcaagtctcc
    44581 tggatgtgcg agcaatgaac ggacagaaaa gaacgcgata actaaggcaa caggtaagaa
    44641 aagataacgt actctgtcca aatattgata gactttttcg tgataaacaa atgcggctac
    44701 cccataaatc atgaaactag caacaaaccg atctaataag taccagctgt catgataagg
    44761 cccagtcaat acttgtgttt gatagaacag ataccatgcg acatataaaa tcgttgaacc
    44821 aatcaatact ggccatacat attttttatt agcaaatact ttgtaaccga gccaaacaaa
    44881 aaacggcatg aacaattgga tctgcagcat cataacggta taccataaat gaggcgctcc
    44941 atcacctgtg acatatttca gtaaaaagct tgcagtatcc gtatacggag tttgttgttg
    45001 gactgctgga aatagccaaa gataagccgt cgtccataga agatacggaa cgactagttc
    45061 aaaaaacttt tctttcaaaa attcccaata agacaaatgc tgacttgttt ttaccatatt
    45121 ataaacaatt gcaaaaataa acattggtgc tgtgtattta acgatgacgt aaaaggtacc
    45181 aagaaaagct gtatttgttg cgctgtggct gtttgccaaa gcaaagctca atatcgtttg
    45241 catcataaca gccgttacag ctgatacttt ctggaagtca cttacgttga ttttgatttt
    45301 tttgttttca aacatctctt tctctctctt tttctttatt accaaagttc tttggttaat
    45361 ttaggtcctt cgactgtttt caatagaatt tttttctctg gctctctcaa atactctttt
    45421 agtgaaaaac cgccttgccg agtcactact tgggtatcga attgctcaaa aaaagcagga
    45481 tgcgattgaa ttacctgaat tggcaattca atcggtgaat ttgcgtacag cagcaaatca
    45541 aaatcactgg tttcttttac tgccggaatc ccagtcgata attcaaatcc taagctgccg
    45601 ccgattcccc atttacatcc tgataatatg tttctagcag catgaaattg ctgataaaca
    45661 ggataatcca tgattttctc ttgtctatac gattcttgtt cgatgatgtc ccacggatga
    45721 atgacttgtt tgacggcatg ttttgagatt tctgttgacc atctctgatt gcgggcaggt
    45781 ccacgtaaac caatcggtag tatttctgcg tgatccgtcg acgcacgacg aactgttccg
    45841 aacatctttt cagttagaac gacccattca ggcaaggtga acccttctat ttcatgttta
    45901 tcaaaaagga caatatcatg tgcttgaatc attttttatg catcccattc ttcgtccatt
    45961 ttggcacgga ctttatttgt ttcgacacgt ccgaatgttt tggctgtttc gtttgtgtat
    46021 cgatttgtca gcatcgtgtc atttgtattg cggacatctt gaatggcttc attgattgct
    46081 tcgatgactt tgctgacatt ttgttctgaa gcagcttggt cggtgatatc ttctaggaaa
    46141 tgatagatcg ctcctagttt tgtataatta ttgatatcgt aagcgatagc tggaactttt
    46201 tcagcagctg cttcgatttc agcaaccgaa cgttttgtca cacgtgcagc actttcttta
    46261 gacatcgctt ggacggtaat ttctttattg tttaaagcaa tcaagcggct tgattgtaat
    46321 ccatgtgcta aaaatgcacc tgaaatagca tttcctgcaa tcaatccaat aactggatgt
    46381 ccagcttgac ggagttttgc ataagcagca gcactattag ccaaagccac atggataccg
    46441 atcaattctt cgttatagcc gtatgcttgg cttggtacat cgatcacaac gatgattggc
    46501 cgtttcactt ttttgtcttt atcttcttcg taaacgtgat ccaaaatttg tcccatgcgg
    46561 aaaccttcaa ctaaaccaac ttcaccgttt cgtacacggt acatcgggtt tttaggatca
    46621 ggtacgattg atgtcacgat ggcttcttct atgaatttac ttgatttacc ataatacaca
    46681 gtactaatag aagaagttgg atttttcatg ccagttagtg ccgcaaacca atcgtaacca
    46741 acacttgctg cagctggttc atttccttct gctacttctg gtaagtcaat tgattttgct
    46801 tgatggttag caattgcttc attgtaatcg tcaatggcca ttggttttga aagatctaaa
    46861 gaatcaagta atgataaata aaatgctgag ctttcactgc gatgcgtgtc ttttcgttcg
    46921 tccattgctg cgatgacagc agctttgatt gcttctacac tatcagtcac taattcatca
    46981 atgatttttg attctacccg ttgacgagat cctaatgtat tccaaatcaa gtctttgtca
    47041 ctagaatcaa attctcgaac gcctgcttct tgttcaataa cttctggtcc attcaaaccg
    47101 actctcgctt tttttgttgc gattaaataa ctcattaatg ctgaagtaat cgacataccg
    47161 ccaaatgatc ctacacgtcc tggaatcaaa ccgatgactg gtacatattg ttttagagca
    47221 atgattacat ttccgatttc tgaaatagat aacagtccgt agtttgcttc ttgcaaacga
    47281 acaccacctg tatctagtac aatgactgga aagatctcat ttcctttttt attttcttct
    47341 aacacatgtt caagtgcagc aacgattttt gcaccggaaa cttcaccgat accgccacct
    47401 tggaattttc cttccatagc gattactact gcttgtttgc cattgatcgt gccacggctg
    47461 acaacgatcc cgtcatcact ttcagggacg attccttgtg ctggtaaact tggcgcgatg
    47521 atttgatcga atggatcaat caattcacgt ccattgtcaa gtagagcgtt cactcgttct
    47581 ctggcgttta attctacaaa actatttttc atctgaaagt tcctccattg cttgcgtcaa
    47641 tcgtaggtac acaactcctg gtgtagcacc gaagtcattg atcacgatat ctgctgaaat
    47701 tggataacga tcaaaaaatc tcgttaatac attgttccat acttcgccga agccatcgct
    47761 tcctgtacaa atttttacag tagattctgt tttttctgtt ggaaacatca agatttccaa
    47821 atctcctgaa ccgaccactc ctacatggat tttcttattg atcggctgtt tcgttggata
    47881 tttataagct aattgttcca tggatctctc ccctttttgt aaaacagagc atacacgttc
    47941 tgcttattcg tctttcttct tccatctggg taaatggcga caaaaggttt ttctcgccaa
    48001 ttttatgacc aagttctgaa tttcattggt ggttcataca ggccatctga ccattcgatc
    48061 aaatcatcga tggtttgtgc agctaataat gaacgtttcg cttcactacg tttgatgcct
    48121 agatcttctg gtaatttcac gatgccggct gcacgcaagc tatctagtga ttctttcgtg
    48181 ctagtcattc caagaggtgt aacaccgcca attgctgcaa ttgctgcttg tcgttcttct
    48241 tttgtttttg ctttgtacaa ataagcaatt ccttcttctg tcacgatatg tgtagtatct
    48301 tcgctataaa tcatcactgg tgcattggct aagcctgctt gtttttgaac agctagtgca
    48361 tctaattctt cgacaaagac tggtttttta tttgaaccat atgtttcaac catttgaaca
    48421 actaattttt ggcctttccc taatggatca tcattgtcgc gaagtgattg ccatgctggt
    48481 gtagaatgac gacgaccgcc aggattatgt cccatgttag gcgcaccgcc aaaaccagat
    48541 aaacgtcctt ttgttacggt agaagagttt ccttcgtaat ctaattgcaa ggttgaaccg
    48601 ataaacatat cgatggcata ttgtcctgca gcttgtgata atgttcggtt actgcgcatc
    48661 gtgccgtctt taccgacaaa aaagacatct gggcgagcag caatatattt ttccatacca
    48721 acttcgccac caaaactatg gatgctttca acccaacctg attcgatggc tggaatcatc
    48781 gttggatgtg ggttcaatac ccagttcttc gcgattttcc cttttaaccc tagtgattca
    48841 ccataagttg gcagtaacaa ttcgatagct gctgtattga atccaatacc gtgattcaaa
    48901 gattgtacgc cgtgttctgc gtagattcct ttgattgcca tcattgccat tagaatttgc
    48961 aattctgtga tgttttgagg atctcgtgtg aataaagctt ctaattgata aggtttatct
    49021 gctttcacga caacgtctac ccagtcacca gggatatcta cacgtggtaa agaatcgaca
    49081 acttcgttga cttgaacgat cacgatacca tctttgaaag ctgcagcttc gatgattgtt
    49141 ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt ctttatctgc tttatctgca
    49201 gctactaaaa caacatttgg aatcaaatcg atgaataagc gtccataaag ttccaaataa
    49261 gtatggatat cacctaattt cattgtgccg tcttcaatca tttgggcgac acgcaagctt
    49321 tgtgcaccag cataagagaa atcgatcttg cttgcgattc ctttttcaaa aatattcaaa
    49381 tgttctggtc gtgagatact agacatgatc aaatgcagat cattcacttt ttctgcatct
    49441 actttttcaa gactttctga aagaaaactt gcttgttttt gattgtcgcc ttctaataca
    49501 actttgtcgc cgggagcaat cagtttttcc attacttcta cgattttttc agtttctaca
    49561 tacttgccat tgagcaagtt gctgattttt tccaatcgct gcgctttttt ttcgcgttta
    49621 gaagcccagt tgcggtttgg tttttccata attagtcatc tccatttttg atatagaaac
    49681 gtggcgtcat caataccatg ttggtctaga ttgatcgtac ggatcccttt atctacacca
    49741 aattttgtat gaatcagctt tgtcaaagta ttgccggggg gaaattcaat cgtcagatcc
    49801 attcctaatt cttttgccaa gtccatcatt tggttccact gcacagggaa agctaggttt
    49861 tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc cgtatttttt
    49921 atatacaaac atctagcatc agatagatgg acagactgca tatacggctt gaatttcttg
    49981 accgtatcat tcatcaagag acagtgagat ggcacaggga ctttcagcag cttcgctgtt
    50041 tgcgcttggt gccgtttggc ttctgccaac gtaacggata aaccttcgat actcccagat
    50101 aacgtatgtt gcatcggaca attttcattt gacagataaa ctggatggtc aggatcaaat
    50161 gtttttgcta caatttcctc agcttcgctt ctcgtcacac ctactactac gcccattgca
    50221 taaccgcttg gataagcttc tttcattaag actgcgcggt gatagactag ttccgctgca
    50281 ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa aagcaccaat cgaatgtcct
    50341 gctgcaatat caggctgaat accgtttttt tccatttcat ctaaataaaa tactgctttt
    50401 gtcagtaatg ctagctggat aaaaaccgta tcttgatagt tttcttcttt atcaactaac
    50461 cgataccccg tcacattttc taaacgttcc atgtacattt cagggatatc cgctaacatc
    50521 cctaacttct gactgccttg tccaggaaat aagaaacctg tactcatgag cctcgcctcc
    50581 tcagcactta ctttttccac tgctatctta gtcaatttct gctataataa aaaatgccaa
    50641 aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg ataattatga aattgcttca
    50701 tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag ccaaagaatt
    50761 atttatttct caacctgcct tgagccgtgc gatcaaacat ttggaaaacg aattaggtgt
    50821 ccctttgttt taccatagcg gcagaaacat tgagacgacc gtttatgcag aagaatttta
    50881 tccatatgca gtcaagacat tagacacatt agaagaagga atcaaattat tgtcttcggt
    50941 caatgagaag atcgttactt ccgtcatatt gtatttagag gtagcctctg tttctattcc
    51001 gaacttggtc cgaattttta tggaaaagca tcctgatatc caattaacga tcatgcaaca
    51061 tcatttacca gatgatatta ccgatcctgt actgtacatc acttcggaga tgaagcctgg
    51121 catgaccaat attcctatca tgaaagaacc tgtttatgtc gccattccta agaaacatcc
    51181 tcttgctaaa aaagaacatc tgcaattaga agatatccaa cgcaccccca tcttgatgct
    51241 gtcaaagagc aacgccttcc gtcatacact ggatgcagca cttgaagcga aaagtatcga
    51301 attgacgatc agttcgacga cagatgatcc tgcgacatta cgttctattg tcaagcaagg
    51361 attagctgtt agctttttcc caaaagtttc ttggtcttac gataaaaatg atcctttcgt
    51421 cttgcgggaa atcaaagatt tgcccttgac acgaacaatt tatttatctt ctagcttcac
    51481 agaagatcat cctttgatca aaatgattgc aaaaacgatg cgggaatttt atttaggaaa
    51541 ataaaaaaaa aagagctggg acaaaattct tgtcgcagct cttttttcga acaaacggtg
    51601 ttccagaagt aatttcttag aaaataagcc gaaatttccc aaaaatttga gaagcaattt
    51661 tcgtaaattc cttcttattt cttgaagcta aacatttctg tcacaacctc ttctctattg
    51721 tcctagacat tcattgcgtt gatttaaaac ttccatcaat caaatctgct agttcatcta
    51781 gcttttctat tttacgtaga tcaatagaga tccaatgttc tttattcatg tgataggctg
    51841 gaaaataccc ctcttttcct cgcaaaattc cgccaagttc aggatcgacc ttcaaatcaa
    51901 gaatattgat gatcctatgg tcacttgcat catcatagat tttttcttct ggaacagaca
    51961 tcaggacccc aaaccatttt ttgctttttt gatgtctgaa tacgcaataa tcaggaaatt
    52021 ttttaaaaat atattctgga gacaaattgt atgtttcatt gatgtgatcg attagttctt
    52081 ttttcgttag catctgcttc catctctttc ttattgattc gctttattca aagataccac
    52141 gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa tacttcgata tttttctttt
    52201 ttatcaactc ttaaatgttc aatccgcctg tgttggtctt gctaccatct actattaaaa
    52261 tgtttattat tgtttgccca cctatttttt gattttagtg gtatcatttt tcaagttcat
    52321 gatgaaattc ttatcatcag taaatgctcc agaaaaataa acaatgattg aaaaatcagt
    52381 tgaaacttga ccaccgttta ttttctccgt attcttagaa tagaagggag tctgattgat
    52441 gtattcacta atgaaaaaaa tcattaccga aaaagatatt cgacgtcatg tttctttagt
    52501 agaacaattg ctgaatcata caaagataac cgtaaatgaa ttagctgaaa tcatagggac
    52561 aaccgaacga acgatttttt ctgaccttca atctattcgc tctcagctgc ccgaaggctg
    52621 ggacatcatc agcgatcaag caggaatcag tttgcaaaac cagcaaaatt tattaacaaa
    52681 tgacctttgg gagatttttt tcaaacaatc agtcagtgtc gaactgctga aaaatctgct
    52741 ttttacaaaa aaagttgctg tccctgattt tctagccgac catgggcttt cttatgggac
    52801 attgaaaaga catgtgacta aaatcaatca gcgtttagca agttacgact tgcagatcga
    52861 tttgacgaaa tatacagctt gtattcttgg aaaagagcgt gcgattcgta ctttttatca
    52921 tcgattgctg attcctttca ctcataacaa ctattttttc gaggattatt cgatccatga
    52981 atcccattat tttcaatttt taagaaacct cagtcaaact gaactagcgg ttgaaacaga
    53041 agagattttc ggaacatgtt ggttctttat caatacgatc cgtataaaag cgaattgccg
    53101 attagattct tctatccata ccaacagcac actctcctcc ctgtatgact ctgcattgaa
    53161 aaaattgtat ttaacggaag gtatttatct taaagacact gagctttctt ttgcttcttt
    53221 ctgctttctg gaaagttgga attacaacaa taactatgga caagaaaccg ctcgctgcct
    53281 gcatcattct ccatttttag aggtcctcga aacgtttgta gaggaactag cttcagagct
    53341 gtctcttgat caactaaaaa aaacatctct gactgataac ttggctctcc tcatattaaa
    53401 atatcatgaa tcgccgatat tgattgaagc attggaccgt caataccatc gttttttaga
    53461 aacatatgat cagcaatatg cacacttgta tcaacagaaa gagaatttac tgaacgatct
    53521 aaaaaaagaa atagaaatca atgaaccaaa ttactttctc caactcttgt ctctactgat
    53581 ccaacaaacg attttttcta tcaaaccgaa tttgttcaat gtctatttta ttttccaagg
    53641 agaaccttca tggaaggcgt tcttgcagca ggaattaaaa gattacttag gaaaacgtgt
    53701 ctgcttctta cctatcgaac taaccgatct ttccactatt tcttttgaaa aaacagatat
    53761 cttgatttcc aatttccctc tagattctct agatatccct attgtataca tttcttctat
    53821 tccaacgaaa aatgagctca atcgtttgac tgaactcact ttcaaaactt ttttatagta
    53881 acggctagcg actgtattct ctagctgttt tttctttttt gcgaaacagt ttgttcatga
    53941 tccctttttc tatttctaca actaagaata gaagaatcac atttgctcca atcaataact
    54001 gttgtgtcat cgttaaactt actgtaccga tcagctgctg gccgactggt agaaaaataa
    54061 ccatcccttg caataaagca aggataccta atgaaagaaa aagcgctttg ttttgcaaaa
    54121 gaccttggtt caatgacggc ttggacaact ctctgcaatt gatcatgtaa acagcttgag
    54181 ctaagacgat attttgcaga agaatggttt gctgtaagct ttgcccttca aactgcatag
    54241 ccaataaata ggcaggaacc atgatcagta gggagacata gatgatccga aacaaatgat
    54301 aaccagatag gattccttgc tttggatctc ttggtgggcg cttcatgatt tctggatctg
    54361 gcgactcgaa ccctagtgca taagataaag tgattgtcgt taccatattg acccataaaa
    54421 tctgtactgg ggacaaaggt agcgggcgat tcagcataag tgcccaaatc aagatcaatc
    54481 cttgagctaa tgcagttggc aagaagaaat tgatggtttt ttgcaaatta tccaaaatcc
    54541 gccggccctc ttttacggct tttgcaatcg tatggaaatt atcatctcct aaaaccatat
    54601 cagctgcctg tttgcttact tcactacctt tgattcccat agcaattcct acatcggatc
    54661 ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccgacgatt tctccatttt
    54721 tctgcaaagc tttaacaatt cgcagtttat gttctggtgt cgttcgagca aagacatcga
    54781 ctattggcac ttgctgaatc aactcttcat cagacataag atccaaatcg atcccttcta
    54841 aaactttggc agtatgtttt aagccaattt gttccgcaat cgcttttgct gtgtctttgt
    54901 gatcccctgt aatcattttc actgagatcc ctgcttgttg gcattccttt acagctttga
    54961 ctgcactttc ttttggcgga tcgatcaatc cggcgatccc tgcaaaagtt aattgggtta
    55021 aagtctcatg cgtgagttcc tgtttcgaat ctactttttt ataagcaaat cctaaaacac
    55081 gctgaccttt tctcgcaaat tctgcagctg tctgactcca tgcttcttgt tctgcagaag
    55141 aaagagttga tttagcaaat aatacttctg gtgcaccttt gacgtaaatc accgctcctt
    55201 tttcctccgc atgcatcgtt gccatatatt tgtattcaga actgaacgga atctttgctt
    55261 ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg aatcagcgca acttctgtcg
    55321 gattgccact taaattttct gttttctgtt cttcttttgt ttttaattcc tgacagtttt
    55381 tcataatgga caacacgcag gcttcttctt ttgcagctac atccacaact gtcatttcat
    55441 tttttgtcaa tgttcctgtc ttatctgaac agatcactgt catagaacct aatgtttcta
    55501 ccgaaggaag tcctttgatg atcgcctttt cttttgccat ttcatgtact ccaacggata
    55561 gaatcatggt caaaacagct ggcaatcctt cgggtaccat tgacaccacc aaagcaatcg
    55621 tagccgaaag aaggatatgc caatccatac catatcgaaa tgtcgtaaaa ataactaaga
    55681 ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt taatcggttg atttttttaa
    55741 tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg attgatttta ccgatttctg
    55801 tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca agcgttcctg
    55861 aaaaacccat atttttctgg tcgccgatcg attcttcttc tgtcaaaacg gcatgacttt
    55921 tttcagatgc ttctgattct cccgtcaaaa tcgattcttc cactaataaa tcatgtgctt
    55981 cgattaatcg gatatctgct ggaacgacat ctccggcacg taagacaatg acatctcctt
    56041 ttaccagttc tttcgcaggg attttttctt gcatcccgtt tgatagtact atcgcttctt
    56101 gtcccatcag ctgcttcaaa ccattcaatg attcttccgc ttttctttct tgccagtaac
    56161 taacgagtcc attgacgatc actacgaata aaataatact tccttcaata tattcactag
    56221 aagcaaattt caaaaaagag gcaatgatca acacgatcat cagcagatca gtaaaatgct
    56281 ttgctatttt tttccatcgt tttgtctgct gccttgcttt tatttcatta tatccttgat
    56341 cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc tgttgatgtt tgatagtttt
    56401 tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct gtttaattga
    56461 tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag gggggtctga
    56521 aataattcgt tttgcaaaat gtatctgggc ctaactgata tttctattgc ttattctttt
    56581 atatacaaaa tttaagaata caaaccagat aaccaataaa aaaggaataa agcgtgtcgt
    56641 gctttcgaat aacaagctga aagcaattga agtaaaaaac accaacaaag caagatcttt
    56701 atatctttgt gtagtgttcc gtttcttttc tttaataaat tgaacatgtg cacacaagat
    56761 cgaagaccac acaaatagaa aacaactcgt tgtcatgcct gccaaaagct gaaaaaacaa
    56821 tcgactatca gctacaaaaa aatgtagaca aagaccggca aaaaataaca ttcctgtaat
    56881 taaaataccg tgtaaaggta cgcctttctt agaaagcgca caaaaccaat ggggcaaact
    56941 tttttctttt gctgtttgat ataaaatcct gctagtactg tagactgcac tatttgctga
    57001 ggacaaagaa gcaaccaaaa tcattagatt gacaattgct gctccgcctt ggcaatggat
    57061 cacttgcagc atagtcacta aaggactttc ttttaaatct aaacaatgcc atggaacaac
    57121 aaataaaatc gacaaaatcg ttcctatata gaaaaagaga atacgccaag gcagacagtt
    57181 gattgctttt ttcaaattct tttgcggatc acttgtctcg ccaacggtta agccgatcat
    57241 ttcaattcca gcaaaactga ataaaaccat aggaaatgag actaccattc cagcaaagcc
    57301 aaacggtagt aattcatcga tctgtccaaa attttctttg attccgatag actgggtatg
    57361 aaaatctgag acactcaatc ctatacccaa taagataaaa aggaaaatcc ccaagatctt
    57421 gatgattgag aaaaaagatt ccaattcacc aaaccacttg actgcaacaa ggttgactgc
    57481 cattaagatc attagtgcgc tacctccgct tatcaaagct ggtacatgta caaaccagaa
    57541 atggatatac aatcctattg ctgttaattc cgcaagtcca gctccaagcc agcaaagcca
    57601 ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa cgaaatcact
    57661 gatagagtta aatgtactat ttgctaaaaa taaatcaccc atggcccgca tcaaaccata
    57721 aatagccaaa ccgcatatca aataaacgag aataacagct ggccctgctg atgcaattgc
    57781 ctttgctgaa cctaaaaata atcctgttcc aattgcgcca cctaatgcga ttaactggat
    57841 atttcgatta gttagagtcc gtttcattgc cggtttttcc atcaagttca cctttttctt
    57901 tttcaagcag catggcgact tcctgttccc aagaaggttt ctttattttt tcaatacaga
    57961 gtggtactac aaacaaaatg acactgatgg ctgtgactgc aattagattg atccatgttc
    58021 cagctttcat cgtcagtccg acagaaaatc cgattgccgc aaataaaaca ataacgacag
    58081 tccaaactcc aagattgctc tttttctctt tttttccaat tctaaatggt cgattcaact
    58141 ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat gatgacatag acgatacagt
    58201 agataacagt agttgaattt acgagcatca aaaatgcttg attgacgcca ggaatcagca
    58261 aataaaaaag tgcaaagatt gagataatga cagactgagt caaaataacg gatttggata
    58321 cgtcaaattg attcgtcttc caaaaattga attttggtgg atacgcacct tttctagcac
    58381 tttctgtcac tgtctttgct ggtcctgagg cccaagcaga taattggact gctacaccaa
    58441 tgaagacaag taaactaaac agattcacaa taagatgtgg caaaccaaga atttggcaat
    58501 aaatcgaaat agattgcgcg atattattca attccatttg tccttttggt acgacatttg
    58561 ctacaactaa agcattcaaa atattgacga aaaatataaa aattaacgcg gcaaatactc
    58621 cgcgaatata cgttttaact ggattttcca atcgagtgat atacacagag gacatttcga
    58681 taccagtaaa aataaacatg atcggcgcaa aataaacaaa tgaggatgca gtcgtagtat
    58741 ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg atcccaactt
    58801 tgatccacgc agcaaaccct aataagagca tcatcgtcaa aggcacgtac aatcctaacc
    58861 aaacaccgac ttttccgccg atttttgcca tatcaaattt caaattcaaa aaagtgatga
    58921 tccaataaac gactaaaatg acaactaatg taaatttgct gttcaaacca agcggcacat
    58981 tatcgataat attaccaaac aacggagcga gtgctgaagc gaccatcacc atcccaggaa
    59041 acatttggac ccataacagc catgagacaa cgaaccccca tttttttcct agagcattcg
    59101 atacccaaag ttctggacca cctttttggg gaaacattcc agcaaattca ccagagatca
    59161 atgagattgg aaaagcgtac aataatactg caaccagcat gtaaaaaatc atcgtccagc
    59221 tcgttgctgc tacatctgga atatttcgta cacttgctac aagggcgcag gtcattccga
    59281 taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt attccttctt
    59341 tcaatagcga catatgtttt tgttttcttt tatttttgtt caagaacaga aatgacaagg
    59401 tttcatcgat tcagtacaaa acccagtatc accgaaacat ccattgaacg atcttagtga
    59461 actgggtttg atacatgaaa cgaacgatcc aacttataga acgttttata aaaaagaata
    59521 tcttggcgag gaatatatga aaaacagggg gaaatacaat tgttttgatg aagcaaataa
    59581 ttgtctgttc tccatatgtc tcttgttatt ctttcacaca aaaatcagtt ctattggatg
    59641 atttcgttta gtttcttttc cattgctttg gcgatttttt ttgtataaaa attgaaaatt
    59701 cgatcatcat tcaaataagg agtcatgata gccgcacgta gcagagtgat tttctgttct
    59761 tttttccatt cttctttcgt tagacccatt ctttcaacaa aacgaatcgg agaatcaccg
    59821 taatcttctt gtgtaaatgt agtatgagaa gtaataaacc gttcaccata aacatcgcca
    59881 tccatataag aagaccgatc gaacattttt tcatttaatt cattgatagc gttcaaatct
    59941 gtacagcctt gttctttgaa tacccaattg accatattaa aatcaggatg attcaatgga
    60001 tagacttcga ttgtttttcc tttaacagta aaagtcagat gatcgagaaa ttctctgaat
    60061 ctttgagctg cctcgattga agccccaatc aattgaccat atccggtcac attcaatggc
    60121 aatacacgat gtgctgtcca gactgcggcg gcggttgctc cagcttttga tccttccaaa
    60181 atataagctc ctaacatatc tggagccttt actgattttt caaacacata tggcgcgaaa
    60241 taagaaatga tatttctcat atttttatgt ttgattacga ttcctccagc agcataaggg
    60301 acatagccca tcttatgagg atctatcgtg acagaatccg cttctgaaat agctctgaac
    60361 ccttcatata cctctttgtc aatcgttacg cagtggtgga aaacatgatg ttcttcaaag
    60421 aattcagcta aggaagcata cggcacaaat tctcctgctt cgtttagaaa cagtgaacgc
    60481 gcatagccgc catatgcggc atcaacatgt aaatagaaat agatcccttc atcttttaac
    60541 ctttctctta attggacgat tttatctaca ctgtctactt gaccttcttc tgtcgttcct
    60601 acgacagcaa ccacacctag aattgggatt ttttgtccag ctaattcacg aattgttttt
    60661 tccaaagcat taatatccat tcgataatcc tcttgaactg ggatagcaac catctgatct
    60721 aagccgactc cgcaaatatc taacgccttc atccaagaat agtgttttgt ttgagggacc
    60781 agccatttcc ctaatctttg gatatttttt ccgctacggg aagaagctgc tttgacctca
    60841 tccatttctt cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac agataaattc
    60901 aaaagttccc actcagacat ttttttcact ttttcaggca gcacttcctt gacagctaaa
    60961 ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa gattagcaat agaaccatct
    61021 gctgcaatgt gtccccatcc gtctgtcata ttgaataatg aagcaaaatc ctgtcctact
    61081 tctgcttcca tttgcgaagt tgccatagaa gattccaatg caacattatt tgggttccac
    61141 agcatcgcat agttgtaagc taataaagca ggcatcaatg tttcagcatt catttgtccc
    61201 cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg actgacttca
    61261 tccaagacct ctatcatatg gtcgcgggtg gctagataat ctggagtgag cttatctcct
    61321 tcgctaattg caggcatatc tgaaggaata taattttctc tccatccaag atgttcatct
    61381 accatcttgt tcaacaacat tttataaact ggtccatttt cagctttgtc gcctataaag
    61441 acggccttga tatccatatc tttcattttt cttccctcca tattttgttg gtcaatgtct
    61501 tgcagataca tgtgtttatc tgtaacacct cttcactata gcgccaaaaa tgagaatttt
    61561 ttttttataa catcaaatcc acgtagattt ttacaaacaa aaataaataa agtcggattt
    61621 ttctgataat tcaacgcaaa aaagcgaaca atttttcaat aaatcagtct tctaaaatag
    61681 ataacctaaa aaaagcgaac aaacaaataa aatgactgtc cttcattcac taaacatatt
    61741 tttatttttt tatattgtta aaaattcgca atttattttt taatagaatt atctgttttc
    61801 caaattttat aacataaaaa acctttttta acggcattta tatgcttttt cagaaattaa
    61861 ctgatgattt caaaaagaaa acatgtcata atatttccca tattgtgcaa aaaaatacaa
    61921 ggcggaggct ctactatgat tgaagaatat attgagaaaa atattttaag acagctgttt
    61981 ttatgtgggc aattctatgt aaataaagaa gtcaatctag aaaagctctc caatctcctg
    62041 catgtttgca aaaccacttt actcaatgat atcaataaca taaaaaaaga atttgaagaa
    62101 cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc agaacatata
    62161 cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa aacttgtctg
    62221 ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga attcatctct
    62281 gtcagcaaag cttattcatt aaaaaaacaa gtattagctt attttaatga ctgcgggatc
    62341 gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct gcttcttaat
    62401 gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt ttttgaacgt
    62461 gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga taaagaaaat
    62521 aaagaaatct tatcgattgg ttttgctatc agtttcctac gccagcaagt gtgtgctgtg
    62581 acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa
    62641 tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt cgcctttatt
    62701 ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat tgcggaagac
    62761 tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga actagttgct
    62821 acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg
    62881 attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga aaaattttat
    62941 cttttaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt ttccagctgg
    63001 tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc ttttgtgatc
    63061 gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat cgtcacaaat
    63121 tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac tttcgatttt
    63181 caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc gcagcagtcc
    63241 tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca atatgaaaat
    63301 attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt gcaaaacaaa
    63361 taaggggctg tgacaaaaat catgtcacag cctcttattt gttgaagatg cccatttctg
    63421 taacaatctc ttgatacacg tcaggctcac aacctcactt attttggaca tctacgggtc
    63481 cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac gccgctagtt
    63541 ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct gaaacaagaa
    63601 tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg ttctcgctga
    63661 ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc ttgattgata
    63721 gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt acaagttttt
    63781 cgaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc ctcttctttg
    63841 tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc gaatgataat
    63901 aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact cgatgatatc
    63961 agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt ttcttgtatc
    64021 tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat caaaaagttt
    64081 gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat ttaaaatagc
    64141 aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta cctactttct
    64201 tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc tatttaaaag
    64261 ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccat ttttctccct
    64321 ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc
    64381 gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa
    64441 atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt atttacagga
    64501 aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg
    64561 cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga ggcgtttttc
    64621 atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct tggcgctgca
    64681 tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga tgaattcgaa
    64741 tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac attggaggaa
    64801 tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat atggcagcgg
    64861 ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt tattcattgg
    64921 atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa tattggaata
    64981 aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc atgcttaatt
    65041 accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt actgagccag
    65101 ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc tttgacaaag
    65161 cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga ttcaataata
    65221 aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa gaaaaactag
    65281 acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa acaaacttct
    65341 ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa gaattgcgtc
    65401 gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg ggcgtcaatc
    65461 gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac ttgaaagaac
    65521 aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa gaaacaccga
    65581 tgcaagatga aatcactgtg actggtttga aaattaccaa tacactgact caagaagaag
    65641 aatacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc attacagatt
    65701 cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat ggtgcagctt
    65761 ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct gacaaattct
    65821 ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa aaccatttgt
    65881 tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg aattcattta
    65941 tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct atcgtcgtgc
    66001 atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg ggttatttcc
    66061 tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg aatggaaaag
    66121 aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca gtgaatattc
    66181 gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc tatatgcctt
    66241 atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc attcctaaac
    66301 attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag atgatcttta
    66361 ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg ggtattccta
    66421 tgtcccgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta gctcgtgccg
    66481 caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa
    66541 gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta ttaccgcaga
    66601 tgcaagcttt tgaacaccgt ttaccaagag gttgtgaaaa agctgccctg catcaagtaa
    66661 taagaacacc caagctaaaa ctgcttctta tgtttttgac ttggttggac ttattaccgc
    66721 agatgcaagc ttttgaacac catttaccaa gaggttatga aaaagctgcc ctgcatcaag
    66781 taataagagc agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac
    66841 cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc
    66901 gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa
    66961 taccgagaaa ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga
    67021 aaacaactat tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa
    67081 ctatttcaat gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata
    67141 cataagacta tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga
    67201 tctcctttaa aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa
    67261 aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa
    67321 cgtagatgat ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc
    67381 catttgcggc tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta
    67441 tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt
    67501 gaaaaaaggg caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg
    67561 cctgaatcac atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct
    67621 tttcggattt ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt
    67681 tccttatgca gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt
    67741 tttatttcta tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa
    67801 aaaaggcgat acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt
    67861 cgctgttatg gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga
    67921 taaagcggct aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg
    67981 aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat
    68041 ggacggatta gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac
    68101 aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac
    68161 tggtgtttat atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat
    68221 cgctgtcaag catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat
    68281 tgcagaagga ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc
    68341 agcacaagca tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa
    68401 accttgaatt cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac
    68461 ccatgagatc gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt
    68521 ctatttccaa aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt
    68581 ctatattttt ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt
    68641 ttatttggct ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat
    68701 ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga
    68761 atttttcaaa tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg
    68821 gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac
    68881 gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg
    68941 atttccgcta atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg
    69001 gcacaaagat ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct
    69061 cctccgacga tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta
    69121 ttagcgattc cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat
    69181 gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta
    69241 tatgtaacca atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc
    69301 tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac
    69361 ttgttctccg ttttttgatt gatcaagcat ctaaagcttc ttttcttctt tcttggatcg
    69421 tagctacctt gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta
    69481 gtacaccacc tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc
    69541 atggaggcat aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac
    69601 taactaagac cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag
    69661 gtaacccaaa catcgttggt tcattaatat tgaaggacac ctggcgcaat cgacaagttt
    69721 gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa cagtaaacct
    69781 aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt gttcacgata
    69841 aacggtgcgc tatgcccagc tgcgattgca ttaacgtttg cttcaatggc tggcgcattg
    69901 attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa ccataagaat
    69961 ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc cataaatggt
    70021 tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc agcaaaaatt
    70081 gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga agcaaatgct
    70141 tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc atttccgctt
    70201 aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa caaaccgttt
    70261 gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg
    70321 gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa agcaatcaag
    70381 acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt ccatgattca
    70441 ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt attgatcatt
    70501 actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc ccgaatcgca
    70561 actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt ctccataaat
    70621 gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg cgttcaatcg
    70681 acgatacaga tcaatgatct cttttgctaa atctgtaaaa gctatagatg tcatcagatg
    70741 atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg cttcttgtgt
    70801 cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat ctttgatttt
    70861 ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca ttgcatcgct
    70921 ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt tttgatcttc
    70981 catttattgt atcctccgat aatttttatt atttgtttat tagtttttct gcttggtcga
    71041 gtactttttc accattcatc atgccatagt cagccatatt gattacatct aaaggaatgc
    71101 ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc ggtcctaaaa
    71161 gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca gatacagcaa
    71221 agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg actaggagac
    71281 ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt ttaaacacgt
    71341 tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa aagcgcttta
    71401 aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact aaaaaagaaa
    71461 gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg tgcacaaaaa
    71521 aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca aaagcttgca
    71581 tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac tttgctattc
    71641 ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt tcaatcagct
    71701 gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca aataaccagt
    71761 aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc aggcaaatcc
    71821 aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa tgttctgctt
    71881 cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc attcattggt
    71941 gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc ttcttctgtt
    72001 gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc atcttgcacg
    72061 attcctttct catccatata ctgttcttca tattctcttc ccataccgtt ttctgttatc
    72121 agccaaggga tattatgata attttctttt agatacatgg ctatatcata catgattttc
    72181 ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc aaagacttca
    72241 taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg ggcgcgaacg
    72301 cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg aatcgtttct
    72361 aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc caggaactct
    72421 tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa caaatcatac
    72481 atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat ttcaggattt
    72541 aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac tgctttagcc
    72601 gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg ttcatgcggc
    72661 cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt gaatgtaaac
    72721 cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt agcataaaaa
    72781 tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc tgggacttca
    72841 taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc gatcatttca
    72901 cttatatgct gagcatattc ttcatctact atacagttct cataatcagt aaaaaaacga
    72961 gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag atccacatcc
    73021 tgctggtatt gttccataaa attagttgct acggctgggc catacccatt atgccaaaca
    73081 aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt ttttcctttc
    73141 caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt tggaatagta
    73201 attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat ttgctacttt
    73261 tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg taataactgc
    73321 accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg gccaagggac
    73381 cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa tcgttccagt
    73441 gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga tcggcatacc
    73501 aaaaataact ggttcattga tgttgaagat cccaggagca agggataact tggtgatttg
    73561 tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga tcgtgactcc
    73621 agacccgccc atactcatat acatatccca aaatggcata gtaataatgt tcggtacttc
    73681 ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca acggttcccg
    73741 tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt gcgcaataaa
    73801 catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc gttgtacgac
    73861 attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa atcccgcagc
    73921 cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag cagaaacatt
    73981 cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca accagcaata
    74041 aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg tatctgtata
    74101 ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta attcataaac
    74161 agttggatta acagataaat aagacatcac tgcgatgata ccagggaata caccatccac
    74221 tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg tcaagaaact
    74281 tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa aacgcaagaa
    74341 acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca atgtgccaaa
    74401 agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca ccatgatata
    74461 ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt tatccataaa
    74521 actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct ttttgtctat
    74581 tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt accaaatata
    74641 aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg tcaattagtt
    74701 tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg gacagtcatt
    74761 gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc tgtatgaata
    74821 tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa agcaattgat
    74881 tcattaatga ttgggataat atgcagcaag gctacgtttc cgctagcttc tgcaatcatc
    74941 gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa agaagtttca
    75001 atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata ttccgctgcc
    75061 aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg tatcatccga
    75121 tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg cttatcactg
    75181 acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag agcccgcaca
    75241 gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata ttcattcggc
    75301 agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt gtcagtagtt
    75361 acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc tgatgtattt
    75421 tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtatac aaaattgatt
    75481 aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac taggataaaa
    75541 ttcactccta gccttcgatc atttttattt tgaacgacaa aaaatatgat gttcaataac
    75601 agacgatctt cattcgattc ttgatgtcat cttttgtttt ttttaatata tggataaaaa
    75661 gtttgattac atcgtttcta ttctagaatt tggtcgattc tcaattcttc ttctggtgag
    75721 aaatctaaat tatccatcat tcgaacactt tcttgtaact gacttaaccg acttgctccg
    75781 accagtacac ttgtaacaga tttttgtcgc aaattccatg caagggccat ttgcgctaat
    75841 gattgtcctc gtgatacggc gattgtttgc agtgctttga ttttttccag agttgatcca
    75901 acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc ttcaggaata
    75961 ccatgtagat aacgatttgt cagcaaccct tgagcaagcg gactgaacgt gattgcccct
    76021 agcccttctt cttctaatac tggagataga tcgtcttcta gtagtgcacg agaaaacata
    76081 ttgtagcgca tctgatgaat aatgaacggt gcttcctttc tttttaaaat ttcagtcatt
    76141 ttttttgtat cttctccatt atagttggat ataccaatat ataatgcttt tccttgtctt
    76201 acaagttgca tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc tgggcgatga
    76261 tgatagaaga tgtcaacata atctagcccc attctttgga gactttggtc acaacttgaa
    76321 atgatggatt tacgagagcc ccattcccca tatggccctt cccacatatg ataacctgct
    76381 ttgctagaaa taatcagttc atcacggtag gcttgaaaat cttctctaaa aatacgccca
    76441 aagtttttct ctgcagctcc agcagggcct ccataattat tcgcaaggtc gaaatgggta
    76501 attcccatgt cgaatgcgcc tcttaaaatc tctctttgat tatggatagg atcataatca
    76561 ccaaaatttt gccataatcc gagagataaa atcggtaatt tcaaacctga atgaccggat
    76621 ttacgatatt ccattttttc ataacgtgca ctatctgcaa tatacaattt ttctcctcct
    76681 catcaaatct ttttaaaacg ctttcttttt tctattttta gtttactaca ttttaaagaa
    76741 aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt ttactcgttg
    76801 atagatcaga aattgattac gtgcttgctg gattttttta taacgtttcg gttgtttttt
    76861 gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt cgatttttgt
    76921 gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt gttccgctaa
    76981 ttctttttgt cgttcgcctt gataaaaaat cactggacga tattgcttcc ctctatcttg
    77041 aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca gcaactcgct
    77101 ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac ctgtgtaacc
    77161 accacttact tgatcataat ttggtttttc aacatgtccg cccgtgtatc ctgacaaaac
    77221 agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac atccccctgc
    77281 aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa agtcatattt
    77341 ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat caggcgtcag
    77401 attcattcgg atagctaatg gacgtagtgt tgcttctagt tttgataact gttctttaac
    77461 ggatatattt tctcttatgt cattctttgt ctgaatcaat acttttcgtt cccaatctcg
    77521 tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt cctcatccat
    77581 agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt tatcataaag
    77641 tgaggaaata agcgattact tcatccccct acagattttt cgtataaaaa agagagaatc
    77701 cttatcgaca gattctcctt tttaatcaat ttattttata aatgaaaagt taatttacct
    77761 tttatggaaa actattttaa aaaataaaca taaatatgca gttaatgtaa caagataaga
    77821 aataatagtc gttactgtat aacttgtgct tactttaata tcagcaactc ctttgactcc
    77881 gcttatttgt accaatccat tagcattcac ctgctccttt acaggttgat gatttatttc
    77941 tacattcgtt cctaaataag catatacagg aagtgtgatt acttggctct tctcattagt
    78001 tactctatat atgatagaac taccagttgt tttataagga aggtttaccc attgactgcc
    78061 aatctttgct tcgtgtttta ttatatgctc cttattctcc aatgaagcga taggagaata
    78121 atcatataaa aaataatcca ttcctttagt ggtatacgcc tctactgttt ctttgtttac
    78181 aacaatatgt cccggctgag aaattatttt ttgattgaaa ttttttttac tagaaacagt
    78241 taaagtgatt aataacaaaa cagtcataaa taaataaaat agttctcttg tcttattgta
    78301 tcttctattt ttgaaaaata tcatcgttaa tatcagagaa gagaatatta tttgcaagct
    78361 taagatccgc caaggaaatt gtaacaattt ggctggcgta ttttgcagaa gataccatgg
    78421 caccaaacta gttgtagcta gccaagtcaa acacgtacca taataaatct ttttttctat
    78481 gtttgttaat tttttaaata aaatcagggg aatacataaa gataacagac caactagtcc
    78541 cattgagtaa gtagttaagt cgttgttcat agcttcagtc agactatcga acacattaga
    78601 tgcatgagca tataaatttg tcacgctagg tttgttgatt ttttggtaga gcatttgttc
    78661 aaacatcgga taccaaaaag cactcgtcag aaccaatgtc attcctgccg cactaaataa
    78721 ttttttaaat atagcaataa aatctgcatt cttgaaacct ttagaaacca aacgaataaa
    78781 aatgataatt aatgctgtac tcattaatgt tgatagtaca tggctgtaca ctagcaaact
    78841 catactcaat gctagcttta cagacggata cttttcttct tttccagaga tgatttgata
    78901 agcataaaat aatacaggga taacaagtgt tattgcgata gcttcggcaa ttgcggaacg
    78961 atagtagatg tctactgtcc gataagcaga gaaaatatag agacaactaa ataaaaatgc
    79021 cgcttttttt tgttttaaaa accgctcgcc ataatgataa gctacgagtg ttgtacacaa
    79081 gttcaataac caaacgtaaa taatatagct tacaatcaaa ttttttgtca tccaatataa
    79141 cattacagct ggaaaaaaag tcagaaaggg ataaaaataa tttacaccat atccagtata
    79201 attgaatgtc tcataattaa tcggactggt aaaaatagtc gataacccct tgattcgaga
    79261 taagtgaaaa gaaagatctc cagtggtata aatactctgt gtatttttga aaaagatgaa
    79321 agaatatata ctactaacta taattaatcc actcaatatt actacttttt tatttatttt
    79381 cattcttctt gtttatctcc tttttagtca catatcgaga atctttcaac ttattaggtg
    79441 caaatcgttt aaaacttgaa tacaccacta aacaataagt cagaatactg atccataaag
    79501 ccatgagagc agttggagta tattttgttg taatttctat cttattttct ccagcattta
    79561 catccacatt cactaagcca tgttttttat aagagtcatt tacgacttta ccgtttactt
    79621 ttacctgtgt acctaaataa gcatatactg gtactgtcac tttttgcttt ttgttgcttg
    79681 ccactgtgta agtaatgtta ttatctgcag ctttatactt ccctttttcc cacgattctc
    79741 ctacttttac ttcatgtttt tctaagtgag gcctttcttt tatcgcttct gttggggcgt
    79801 aatcgtataa tcctcccata tctgatgttg tataacgtgg gagtgtttct tcattgataa
    79861 caatatgacc gtgttttgat tggataactt ttgcgtaatt ttcttttgct gctatagacg
    79921 tgacgagtag aaacaccaca gtagcgccaa tgattaacca tgaatatttt ttattcatag
    79981 tctttttcat aaaaacaagc gttatgatca ttgaagaaaa aaagatttgg atacctaaga
    80041 tacgccaagg aaactggagc aagcttatcg gtgttttttg aaacaaaaac catggaaata
    80101 aattagttac gcatacccat gtagaaacta cgccaacata gatgattttt tcttttttct
    80161 ccatttcttt cagtatgaat aaaggaaaaa tcaacgcaac caatcctaat aaccccatag
    80221 tgtatgttgt aatatcgtta tttaacgctt gaatgatact atctgaaata gaaagtgctt
    80281 ctagctgcaa atctgttttg aagggactgt gtatttcttg ataattcatc tgctgcaaca
    80341 taggatacca aaaatagcta gttaatgcac aagtcaaaaa aacagccttt actaaattca
    80401 cgaccacaaa ttttatatcg ttttttattt gcttgcttaa aaacaaacga actataatca
    80461 tacaagcaat caatgcacta cacaatagtg tagttaagac atgagtatac acaagtagac
    80521 ttatactgac agataaagcg atgacttttt tttctctatt gaatatcaat ccataagcat
    80581 aatataaaac agctgggata atcgtaagag cgattgcttc tgcaattgcg gatcgataat
    80641 agatatcaac tgttcgataa gttgcaaaag tatataaaca actaaataaa aaagcagcat
    80701 ctatcttttt aaaaaacttt tctccataat aaaaac
//
LOCUS       DACCCM010000004        80101 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCCM010000004 DACCCM010000000
VERSION     DACCCM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.004"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            346..3180
                     /locus_tag="IVK78_000238"
     CDS             346..3180
                     /locus_tag="IVK78_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6373738.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3371..3862
                     /locus_tag="IVK78_000239"
     CDS             3371..3862
                     /locus_tag="IVK78_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP6373739.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3965..4954
                     /gene="manX"
                     /locus_tag="IVK78_000240"
     CDS             3965..4954
                     /gene="manX"
                     /locus_tag="IVK78_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP6373740.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4987..5790
                     /locus_tag="IVK78_000241"
     CDS             4987..5790
                     /locus_tag="IVK78_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP6373741.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5811..6725
                     /locus_tag="IVK78_000242"
     CDS             5811..6725
                     /locus_tag="IVK78_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP6373742.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6808..7182
                     /locus_tag="IVK78_000243"
     CDS             6808..7182
                     /locus_tag="IVK78_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP6373743.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7321..7566
                     /locus_tag="IVK78_000244"
     CDS             7321..7566
                     /locus_tag="IVK78_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6373744.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7738..8802
                     /locus_tag="IVK78_000245"
     CDS             7738..8802
                     /locus_tag="IVK78_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6373745.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9048..9473
                     /locus_tag="IVK78_000246"
     CDS             9048..9473
                     /locus_tag="IVK78_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6373746.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9466..9915
                     /gene="nusB"
                     /locus_tag="IVK78_000247"
     CDS             9466..9915
                     /gene="nusB"
                     /locus_tag="IVK78_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP6373747.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10012..10866
                     /locus_tag="IVK78_000248"
     CDS             10012..10866
                     /locus_tag="IVK78_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP6373748.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10867..12204
                     /locus_tag="IVK78_000249"
     CDS             10867..12204
                     /locus_tag="IVK78_000249"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP6373749.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12209..12433
                     /locus_tag="IVK78_000250"
     CDS             12209..12433
                     /locus_tag="IVK78_000250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP6373750.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12435..13325
                     /locus_tag="IVK78_000251"
     CDS             12435..13325
                     /locus_tag="IVK78_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6373751.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13334..14152
                     /locus_tag="IVK78_000252"
     CDS             13334..14152
                     /locus_tag="IVK78_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP6373752.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14259..14708
                     /locus_tag="IVK78_000253"
     CDS             14259..14708
                     /locus_tag="IVK78_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6373753.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14726..16408
                     /gene="recN"
                     /locus_tag="IVK78_000254"
     CDS             14726..16408
                     /gene="recN"
                     /locus_tag="IVK78_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP6373754.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16463..16579)
                     /locus_tag="IVK78_000255"
     CDS             complement(16463..16579)
                     /locus_tag="IVK78_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP6373755.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16652..17608)
                     /locus_tag="IVK78_000256"
     CDS             complement(16652..17608)
                     /locus_tag="IVK78_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6373756.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17779..18147
                     /locus_tag="IVK78_000257"
     CDS             17779..18147
                     /locus_tag="IVK78_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP6373757.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18264..18749
                     /gene="mraZ"
                     /locus_tag="IVK78_000258"
     CDS             18264..18749
                     /gene="mraZ"
                     /locus_tag="IVK78_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP6373758.1"
                     /translation="MGDCGRLILVSGGMGGLSMLMGEFQHNIDAKGRLIVPSKLREEL
                     GEKFVLTRGLDGCLFGYPMSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQ
                     GRINIPSTLRNYAALTKGCVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFG
                     L"
     gene            18770..19729
                     /gene="rsmH"
                     /locus_tag="IVK78_000259"
     CDS             18770..19729
                     /gene="rsmH"
                     /locus_tag="IVK78_000259"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP6373759.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19734..20135
                     /gene="ftsL"
                     /locus_tag="IVK78_000260"
     CDS             19734..20135
                     /gene="ftsL"
                     /locus_tag="IVK78_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP6373760.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20136..22328
                     /locus_tag="IVK78_000261"
     CDS             20136..22328
                     /locus_tag="IVK78_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6373761.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22354..23316
                     /locus_tag="IVK78_000262"
     CDS             22354..23316
                     /locus_tag="IVK78_000262"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP6373762.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23330..24691
                     /locus_tag="IVK78_000263"
     CDS             23330..24691
                     /locus_tag="IVK78_000263"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP6373763.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24717..25805
                     /gene="murG"
                     /locus_tag="IVK78_000264"
     CDS             24717..25805
                     /gene="murG"
                     /locus_tag="IVK78_000264"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6373764.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25820..27040
                     /locus_tag="IVK78_000265"
     CDS             25820..27040
                     /locus_tag="IVK78_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP6373765.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27196..28521
                     /gene="ftsA"
                     /locus_tag="IVK78_000266"
     CDS             27196..28521
                     /gene="ftsA"
                     /locus_tag="IVK78_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP6373766.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28544..29785
                     /gene="ftsZ"
                     /locus_tag="IVK78_000267"
     CDS             28544..29785
                     /gene="ftsZ"
                     /locus_tag="IVK78_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP6373767.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29802..30479
                     /locus_tag="IVK78_000268"
     CDS             29802..30479
                     /locus_tag="IVK78_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP6373768.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30493..31092
                     /locus_tag="IVK78_000269"
     CDS             30493..31092
                     /locus_tag="IVK78_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP6373769.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31107..31394
                     /locus_tag="IVK78_000270"
     CDS             31107..31394
                     /locus_tag="IVK78_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP6373770.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31443..32225
                     /locus_tag="IVK78_000271"
     CDS             31443..32225
                     /locus_tag="IVK78_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP6373771.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32246..32953
                     /locus_tag="IVK78_000272"
     CDS             32246..32953
                     /locus_tag="IVK78_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP6373772.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33215..36013
                     /gene="ileS"
                     /locus_tag="IVK78_000273"
     CDS             33215..36013
                     /gene="ileS"
                     /locus_tag="IVK78_000273"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP6373773.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36062..37588)
                     /locus_tag="IVK78_000274"
     CDS             complement(36062..37588)
                     /locus_tag="IVK78_000274"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP6373774.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37603..38250)
                     /locus_tag="IVK78_000275"
     CDS             complement(37603..38250)
                     /locus_tag="IVK78_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP6373775.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38434..38763)
                     /locus_tag="IVK78_000276"
     CDS             complement(38434..38763)
                     /locus_tag="IVK78_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP6373776.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38940..39668
                     /gene="sfsA"
                     /locus_tag="IVK78_000277"
     CDS             38940..39668
                     /gene="sfsA"
                     /locus_tag="IVK78_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP6373777.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39684..40697
                     /locus_tag="IVK78_000278"
     CDS             39684..40697
                     /locus_tag="IVK78_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6373778.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40697..41974
                     /locus_tag="IVK78_000279"
     CDS             40697..41974
                     /locus_tag="IVK78_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6373779.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42037..44739
                     /locus_tag="IVK78_000280"
     CDS             42037..44739
                     /locus_tag="IVK78_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6373780.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44891..45208
                     /locus_tag="IVK78_000281"
     CDS             44891..45208
                     /locus_tag="IVK78_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6373781.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45238..45558
                     /locus_tag="IVK78_000282"
     CDS             45238..45558
                     /locus_tag="IVK78_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6373782.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45666..47126
                     /locus_tag="IVK78_000283"
     CDS             45666..47126
                     /locus_tag="IVK78_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6373783.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47194..47415
                     /locus_tag="IVK78_000284"
     CDS             47194..47415
                     /locus_tag="IVK78_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373784.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47446..47628
                     /locus_tag="IVK78_000285"
     CDS             47446..47628
                     /locus_tag="IVK78_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP6373785.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47628..48041
                     /locus_tag="IVK78_000286"
     CDS             47628..48041
                     /locus_tag="IVK78_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP6373786.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48164..49345
                     /locus_tag="IVK78_000287"
     CDS             48164..49345
                     /locus_tag="IVK78_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6373787.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49734..49807
                     /locus_tag="IVK78_000288"
     tRNA            49734..49807
                     /locus_tag="IVK78_000288"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49768..49770,aa:Arg,seq:cct)
     gene            complement(49876..51015)
                     /locus_tag="IVK78_000289"
     CDS             complement(49876..51015)
                     /locus_tag="IVK78_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6373788.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51314..51949)
                     /locus_tag="IVK78_000290"
     CDS             complement(51314..51949)
                     /locus_tag="IVK78_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373789.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52062..52697)
                     /locus_tag="IVK78_000291"
     CDS             complement(52062..52697)
                     /locus_tag="IVK78_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP6373790.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52731..53192)
                     /locus_tag="IVK78_000292"
     CDS             complement(52731..53192)
                     /locus_tag="IVK78_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP6373791.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53322..53753)
                     /locus_tag="IVK78_000293"
     CDS             complement(53322..53753)
                     /locus_tag="IVK78_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6373792.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53771..54091)
                     /locus_tag="IVK78_000294"
     CDS             complement(53771..54091)
                     /locus_tag="IVK78_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6373793.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54390..55166
                     /locus_tag="IVK78_000295"
     CDS             54390..55166
                     /locus_tag="IVK78_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6373794.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55181..55384
                     /locus_tag="IVK78_000296"
     CDS             55181..55384
                     /locus_tag="IVK78_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6373795.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55400..55738
                     /locus_tag="IVK78_000297"
     CDS             55400..55738
                     /locus_tag="IVK78_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP6373796.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55725..55904
                     /locus_tag="IVK78_000298"
     CDS             55725..55904
                     /locus_tag="IVK78_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373797.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(55947..56417)
                     /locus_tag="IVK78_000299"
     CDS             complement(55947..56417)
                     /locus_tag="IVK78_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373798.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56504..57202
                     /locus_tag="IVK78_000300"
     CDS             56504..57202
                     /locus_tag="IVK78_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP6373799.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            57380..57721
                     /locus_tag="IVK78_000301"
     CDS             57380..57721
                     /locus_tag="IVK78_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373800.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            57714..58385
                     /locus_tag="IVK78_000302"
     CDS             57714..58385
                     /locus_tag="IVK78_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP6373801.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            58391..59077
                     /locus_tag="IVK78_000303"
     CDS             58391..59077
                     /locus_tag="IVK78_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373802.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            59080..59829
                     /locus_tag="IVK78_000304"
     CDS             59080..59829
                     /locus_tag="IVK78_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6373803.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            59841..60110
                     /locus_tag="IVK78_000305"
     CDS             59841..60110
                     /locus_tag="IVK78_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373804.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            60273..60572
                     /locus_tag="IVK78_000306"
     CDS             60273..60572
                     /locus_tag="IVK78_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373805.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            60572..60874
                     /locus_tag="IVK78_000307"
     CDS             60572..60874
                     /locus_tag="IVK78_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373806.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            60914..61279
                     /locus_tag="IVK78_000308"
     CDS             60914..61279
                     /locus_tag="IVK78_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373807.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            61906..62094
                     /locus_tag="IVK78_000309"
     CDS             61906..62094
                     /locus_tag="IVK78_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373808.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            62102..62296
                     /locus_tag="IVK78_000310"
     CDS             62102..62296
                     /locus_tag="IVK78_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373809.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(62329..62541)
                     /locus_tag="IVK78_000311"
     CDS             complement(62329..62541)
                     /locus_tag="IVK78_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373810.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            62666..62869
                     /locus_tag="IVK78_000312"
     CDS             62666..62869
                     /locus_tag="IVK78_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373811.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            62866..63138
                     /locus_tag="IVK78_000313"
     CDS             62866..63138
                     /locus_tag="IVK78_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP6373812.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            63139..63324
                     /locus_tag="IVK78_000314"
     CDS             63139..63324
                     /locus_tag="IVK78_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373813.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            63311..63658
                     /locus_tag="IVK78_000315"
     CDS             63311..63658
                     /locus_tag="IVK78_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373814.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            63618..64094
                     /locus_tag="IVK78_000316"
     CDS             63618..64094
                     /locus_tag="IVK78_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP6373815.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(64240..64968)
                     /locus_tag="IVK78_000317"
     CDS             complement(64240..64968)
                     /locus_tag="IVK78_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP6373816.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            65016..65294
                     /locus_tag="IVK78_000318"
     CDS             65016..65294
                     /locus_tag="IVK78_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373817.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            65296..65682
                     /locus_tag="IVK78_000319"
     CDS             65296..65682
                     /locus_tag="IVK78_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373818.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            65679..66059
                     /locus_tag="IVK78_000320"
     CDS             65679..66059
                     /locus_tag="IVK78_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6373819.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            66171..66623
                     /locus_tag="IVK78_000321"
     CDS             66171..66623
                     /locus_tag="IVK78_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP6373820.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            66620..68347
                     /locus_tag="IVK78_000322"
     CDS             66620..68347
                     /locus_tag="IVK78_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6373821.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            68411..69598
                     /locus_tag="IVK78_000323"
     CDS             68411..69598
                     /locus_tag="IVK78_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6373822.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            69564..70139
                     /locus_tag="IVK78_000324"
     CDS             69564..70139
                     /locus_tag="IVK78_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP6373823.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            70152..71516
                     /locus_tag="IVK78_000325"
     CDS             70152..71516
                     /locus_tag="IVK78_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6373824.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            71518..71799
                     /locus_tag="IVK78_000326"
     CDS             71518..71799
                     /locus_tag="IVK78_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP6373825.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            71777..72103
                     /locus_tag="IVK78_000327"
     CDS             71777..72103
                     /locus_tag="IVK78_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6373826.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            72093..72431
                     /locus_tag="IVK78_000328"
     CDS             72093..72431
                     /locus_tag="IVK78_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373827.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            72421..72786
                     /locus_tag="IVK78_000329"
     CDS             72421..72786
                     /locus_tag="IVK78_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6373828.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            72793..73419
                     /locus_tag="IVK78_000330"
     CDS             72793..73419
                     /locus_tag="IVK78_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6373829.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            73419..73883
                     /locus_tag="IVK78_000331"
     CDS             73419..73883
                     /locus_tag="IVK78_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373830.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            74078..76387
                     /locus_tag="IVK78_000332"
     CDS             74078..76387
                     /locus_tag="IVK78_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6373831.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            76399..77103
                     /locus_tag="IVK78_000333"
     CDS             76399..77103
                     /locus_tag="IVK78_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6373832.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            77100..79859
                     /locus_tag="IVK78_000334"
     CDS             77100..79859
                     /locus_tag="IVK78_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6373833.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            79872..>80101
                     /locus_tag="IVK78_000335"
     CDS             79872..>80101
                     /locus_tag="IVK78_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP6373834.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
BASE COUNT        27132 a        13158 c        16844 g        22967 t
ORIGIN      
        1 aaataaaagt tgtagtggag gctacgaagt ttactttgtc tttttacact taatattttt
       61 ttgccaattt ggaatcagga tgttttgata actatcgttt tcaattgatg gaagctatat
      121 tgtttgctat cagtgaaata attatatggt aaacagtgtt ctttttcttg tttccattat
      181 aaaaatcgta tcgggatttt tcgttctttt aaattgaaca tttctatcta tccgcgtgtt
      241 atcaacaaaa caaaatcagg taaatagttg acgaaacaga aataaataca tagtattatg
      301 ttttgaagcc taagtacaat agtctataga aaaggagcga taccattggc aaaacgcatt
      361 gaccgaattt atatgtatgt caaagaaaaa accgctcatt taacaaaggc agaatatgat
      421 caaggagtaa caacacagga aattgctgaa gtgttaggga ttcagcgaac aaatagcagt
      481 aaagatctga atcagctagt gcgcgaagga aaactgataa agacagacgg acgaccagta
      541 cgttacatat atcaaacact cgttacgcac gtaaagccag ttacaaaaca tgtagtcagt
      601 tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc aaatagatac taaggatatc
      661 tttgcaaaaa tcattggagc taacggcagc atgaaaaatt cagtggaaca ggcaaaagct
      721 gcgattttgt atccgccaag aggattaaat tgtttgatta caggacctac cggatcagga
      781 aagacttatt ttgcacacgc gatgtatcat tttgcaaaag cgcatcatgt aatagatgag
      841 gaaacggaac tgattgtttt caattgtgct gattatgcca ataaccctga gcttttgatg
      901 agtcatttat ttgggtacgc aaaaggtgct tttacaggcg cagatgaaga aaaaatgggg
      961 attatcgatc aagcggatgg cggtatgctg tttttggatg agattcaccg gttgccgcct
     1021 gaaggtcaag aaatgatctt ttactttatg gatcatggaa cttacagtcg tcttggggaa
     1081 acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg cgaccacaga agacccaggt
     1141 tcttctctat tagaaacttt tgttcggcgt atcccaatca atatcaaact tccggctttt
     1201 gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa tcatgattgc ccacgaagca
     1261 aaccgcacgc agcgaaagat ttctttgaca gaagatgtag caaaagcatt gatcggcagt
     1321 gtgacctatg gaaatatagg acaattgaag tcaaatatcc agttagtatg tgcacgagga
     1381 tttctaaatc atatgcaatc acccgaaatc tcgatcacaa tcgatgattt gacagaaggg
     1441 attcgtagcg gattgattca attagcaagc aaccgcagtg caatggcaga gctttctaaa
     1501 atattggagc caaagataac tgtagcacca aatgatacga tcatgaaaat ccaatcggac
     1561 tcctatgagt taccttataa tctatatgat atcatcggag ataaagcagc tttactgaag
     1621 tcggatggtt tggatcaaga agcaatcaat catttcattt ctacagatat caacatccat
     1681 ctgaagtctt tctataaaga ccatggtttt tcttttaatg cagacagcaa attagcagaa
     1741 tttgtagatc cgaaaatcat agaagtgacc aatcagattt atgtaatggt caaaagatct
     1801 ttaccatatg agtttcagca aaactttatt tatgctatga gcttgcatat cagttctttt
     1861 ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg ataacatccg tgagatggct
     1921 tttgattttc cagatgaata tgctgttgcg aaagaagtgc gtagttatat cgaaacgtat
     1981 tttcaagtgg agattccaga aagtgaagac tattacttga ccgttctact cgtctcgtta
     2041 cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg cagctcacgg aaacagcaca
     2101 gcaagcagta tggtccaagt tgtccaacag ttgttagatg cagagaacgt acgagcagta
     2161 gacatgcctt tggatatgga tccgaaaaca gcattggacc gaattgaacg caatgtacaa
     2221 gaagtagatg aaggaagcgg ggtcatactc ttagtagata tggggtccct tgcgtcgttt
     2281 aattcgcaaa ttcaaagaga cactggaatt ccagtacgta ctgttgatat ggtaacaact
     2341 tctctagttt tggaaactgt acgaaaggtc tcagtattag ggacagatct agatatgttg
     2401 catgaatcat tgaaaaattt tcgaggttac gcagaaatat cttctgtaga atctgttcag
     2461 gaagcaaaac ctattcataa gaaaaaagct gttttggcta tctgcgcatc tggcgaaggt
     2521 acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa acaagcgtca agaaacagaa
     2581 ttagaagttc tagctttgtc gattgtggaa gtgaaagaag aactgccagt tatccaaaaa
     2641 gaatatcaga tcatagctac tactgggatt actgatttga atatccaagc cccatttatt
     2701 cctttagaac gttttattga tcaaaatatc gaagtcattt tggatcagtt gctgatggaa
     2761 tcagaattgg aggaaactga attcatctcc ctagatgaag agtcagctaa aaatacgtgt
     2821 gttgagttta tctctgataa cttcatcttt attaatggta gtaagttaat cgatccaatg
     2881 tggcaatttt ctacacaaat ctctcagacg acgggaatcg gcgatgaaga atatggattc
     2941 aaaatcaacc tagtgatgca cacagcagga atgatcgaac gtattattcg gaatgaacca
     3001 ttgactgtgg aagaaaacga attgacaaat acaacgaatg atcctcttta tagccaattg
     3061 gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa aagtgccgat agaagaaatg
     3121 tattatctgc tacgtctagt ccataatcag ttagataaaa aagaatacac tgtgccatga
     3181 atacacaaaa acgatacact tttatagtgt atcgtttttg tgtattggtt aagaatcctt
     3241 taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt ttgcttatag tagtgtgtgc
     3301 taggaggtgg ttgttcacac agaaatgata ggggtttctg tgaagtagtg aggaagaagg
     3361 agttttgcag atggatattc gattggcacg tatagatgat cgtttaatcc acgggcaagt
     3421 cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc attgtgatca gcgatgtagt
     3481 cgctcaagat aaattgagaa agtttctact aaaagaagct gcaccgccgg ggatcacagc
     3541 aaatgtaata accgtgcaga aaatgattca aatctatcat agtggattat tatcacgagc
     3601 aaaagtgatg cttttattca ccaatcccca agatgttgaa gcggttgtcc gctgcggtgt
     3661 acagttgaag tcgttgaata tcggcggtat gagttttact gaaggcaaat caatgattac
     3721 caattttgtt tcagtaaatc aacaagatat tgattccttt cgttacttga acagtcaaga
     3781 agtggagtta gagattcgaa aagtacctgc tgacaggaaa atccttttga tggaactact
     3841 agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa aaggtcaaat aattctgttg
     3901 taatggttag caattattaa tatttttgaa tttgttacaa caaaagtaaa taggaggtat
     3961 aaatatggta gggattatcc tagcaagtca tggacaattt gctgaaggaa tcttacaatc
     4021 cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc gtgattttga aaccaagtga
     4081 aggtccagat gacttgagag ccaaattgga agaagcggtt gcttcttttg ataatcaaga
     4141 cgaagtgtta ttcttagttg atttatgggg cggaacaccg ttcaatcaat caaacacatt
     4201 attcgaagag cacaaagata aatgggcaat cgtcagcggt ttgaatttac caatgttgat
     4261 tgaagcctac gcatcacgct tttcaatgga atctgctcat gaaattgcag cacacatcat
     4321 tgaaacagca aaagacggtg taaaagtaaa accggaagaa ttagaaccgg cagaagcacc
     4381 gaaagcagca gttgaagacg cacaacctaa aggagcactt ccagaaggaa ctgtggtcgg
     4441 agacggaaaa atcaaatatg tattagcacg tgttgactca cgtctgttac atggacaagt
     4501 tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt atcgttgttt cagatgcagt
     4561 atcaaaggat gatctgcgta agagactaat cgaacaagct gctcctccag gtgtaaaagc
     4621 aaatgttatt cctattagca aaatgatcga agtagcaaaa gaccctcgtt ttggaaatac
     4681 caaagcattg cttttatttg aaaatcctga agacgtattg acagctgttg aaggtggcgt
     4741 agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg gtcggtaaag tcgttgtaag
     4801 taaagtattg tctatgggac aagaagacgt tgaagcgttt gagaagcttg aacaaaaagg
     4861 tgtgaaattc gatgtacgta aagtacctaa tgattcacgc gataacatgg atgatatttt
     4921 gaaaaaagca aaagctgaat tagcaaaagc gtaataaata atattaaaat ttataggagg
     4981 cttatcatgt ctattatttc aataatttta gtcgtattag ttgcctttct agctggtatg
     5041 gaaggtattc ttgatgaatt tcaatttcat caaccactag tggcatgtac attgatcggt
     5101 ttagttaccg gtaacctcga agctggtatc gtacttggcg gtacgcttca gatgatcgct
     5161 ttaggttggg caaatatcgg tgccgctgta gcaccagatg ctgcattagc atcagtagca
     5221 tcagcaatta ttttagtttt aggtggacaa ggtgttaaag gtgttccttc tgcgatcgcg
     5281 attgccgttc ctttggctgt tgcagggttg ttcttaacaa tgatcgtacg tacaatcgct
     5341 gttccaatcg ttcatttgat ggatgcggct gctgaagaag gaaacatccg taaggttgaa
     5401 atgtggcaca tcattgctgt atgtttacaa ggggttcgta tcgcggtccc agcagcagcg
     5461 ttgctattta tcccagcaca aactgtgcaa tcattccttg aatcaatgcc agcatggtta
     5521 acagatggta tggctatcgg tgggggaatg gtcgtagctg taggttacgc tttggtaatc
     5581 aacatgatgg ctacaaaaga agtatggcca ttcttcgtta ttggtttcgt tgtagctgca
     5641 atttctcaat taacattgat tgctctaggt gctttaggtg ttgcattagc tcttatttac
     5701 ttgaaccttt ctaaaatggg tggttcatca aataatggag gcggaagcaa ctctggtgac
     5761 cctttaggcg acattttgaa tgactattaa tcttgaagga ggaaaataaa atggcagaag
     5821 aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc gtggcgctct accttcattc
     5881 aaggttcatg gaactacgaa cgtatgcaaa acggtggctg ggcattttct atgattccag
     5941 caatcaaaaa attatacaaa acaaaagaag aacgttctgc agcattaaaa cgtcacttgg
     6001 aattctttaa tacacaccca tacattgctt ctccaatttt aggggttaca ttggctctgg
     6061 aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc gattcaaggg gttaaagtag
     6121 gtatgatggg tcctttagct ggtgttggtg acccagtgtt ctggtttaca gtacgtccaa
     6181 tgctaggtgc actaggtgct tcattagcta tgggaggaaa tatccttgga ccaatcatct
     6241 tcttcttagg ttggaacatt attcgttggg cattcatgtg gtatacacaa gaatttggct
     6301 acaaagctgg ttctaaaatt actgatgacc tttctggcgg attgttacaa gacgtaacaa
     6361 aaggtgcttc aattttaggg atgttcgtat tagctgccct tgtacaaaga tgggtatcaa
     6421 tcaaattctt accagttgta tcaactgtaa aattagataa aggtgcttac atcgaatggg
     6481 ataaattgcc ttcaggtggt gaaggaatca agagtgcatt tgaacaagtt aataatggct
     6541 tagccctttc tcctgaaaag gtaacaacat tacaagacaa cttagaccaa ttgattcctg
     6601 gtttagctgc attaggatta acattgttat gtatgtggtt gcttaagaaa aaggtttctc
     6661 caatcgttat catccttggt ttattcgtag tcggtgttgt tggacacgtc atcggacttt
     6721 tataatccat aaaaatatag ctcgggcgtt gtcccgagct ttttttatat agtatactgt
     6781 tcataaacaa atatctggag ggagaaaatg gttcaatcaa tcaatacaaa agtagacttg
     6841 gtaattgatg ctacttcgtt tacgggtctg acagattacg ggcaaatcat gataggtgat
     6901 aatggttttg agttttacca tgcgcgagat agacgcaaat atatccaaat tccatgggaa
     6961 gaagtggatt atgtactagc atcagtcatg ctaaagggaa aatggattcc tcgatatgcc
     7021 attcaaacga aaaaaaatgg tacatacaca ttttcttcca aagaacctaa aaaagtgctt
     7081 cgtgcgatca gagaatatgt agatgaagat agaatggtac aatcattgag tttctttgat
     7141 gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt aaaaaataaa gaaaacttct
     7201 aagaggaggt tttctttatt tttttacttt gcagaagaaa atatgaaata gcaaaaccaa
     7261 ttatttgtcc cctttaaatc ccatatgcta aaattgatta gcaaagaagg aggttatatt
     7321 atgagttggt tatggacatt gattgttgga gccgtgatcg gagcaattgc aggtgcgatc
     7381 accagtaaag gaaattcgat gggctgtgta acgaatatca ttgctggact agtcggttca
     7441 agtattggac aagcgatttt cggcgaatgg ggaccaaaag ctgcaggtat ggcacttatt
     7501 ccatctattt taggcgcagt gattttggta gcagttgtct catttttctt tggaaaacga
     7561 tcataagttt ttacaaaata gtaaactaaa aggagttgca gcgagactgt gactcctttt
     7621 tggctttatt tatttaaaat aaaaagaagt tttccggaaa tttttattta actgtcgtgt
     7681 acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag aaagagggag tttcattatg
     7741 atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg
     7801 gttaccagtc cgtacaatct tcgttactta acgaacttta caggtacgac aggattagct
     7861 gttattactt tggaaaaagc attttttatt actgattttc gttataccga gcaggcggca
     7921 gcgcaggctc aagggtttga aattattaaa aatgtcggac ctatttttga tgaggtagca
     7981 aacttagtac gcaaagaaga actgcactca ttagcttttg aggaaacaac agtctcattt
     8041 ttagaatatt cggttttaga agaaatcatc gatgcggaat tagtacctgt ttcacagatg
     8101 atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt
     8161 catattgcgg atatggcata tgatcacatc ctgaaaatga tccaaccggg catgactgag
     8221 attgaagtcg caaatcaact agacttttat atgcgttctc ttggcgcttc aggtgtttct
     8281 tttgatacga tcgttgctag cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa
     8341 aaaatcatcg aacaaggtga cttgatcaca ttggactttg gttgctatta cgaagggtac
     8401 gtttccgata tgacacgtac attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt
     8461 tatcaaatag tcttagatgc tcaattagca gttattgatg ctgcaaaacc aggaatgact
     8521 ggtgtccaat tagatgcagt tgctagagat tatatttcaa aacatggtta cggggaagca
     8581 tttggacatt ctacaggaca tgggatcggt ttagagatcc acgaaggacc aaacgtttct
     8641 tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca ctgatgaacc agggatctac
     8701 ttaccaggaa tcggtggagt acgtattgag gatgacttgt tgatcacaga agatggaaat
     8761 cgtgtgttga ctcgttcacc aaaagaattg atcatcttat agtttcatca atagaagaag
     8821 taagcgtaaa aatcagtgtt cacagacttt gttatagaaa atcactcgat ttaatgatac
     8881 actatagaag aacttttaag ctgtaagaga taataaagtt gggtgttttc tccaattcat
     8941 agcagtttgc agctgatcgg cggcagtttt cttaccacgt gatcactatg ccgcatcata
     9001 gagaattcgg atgtatctcg tgcttaggca aagggaggaa taaataaatg gcagatgaaa
     9061 aaaatttgat tttaaacgca aaccaagatt taggagagat cgtgatcgct cctgaagtca
     9121 tcgaagtaat tatcgggatt gcagcttcta aagtagaagg cgtatacgga atgcgcggaa
     9181 cttttgccag caatgtcact gaattattag gacgtgcagc tcacggaaaa ggtgtttact
     9241 tagcaaacga ggaagatggc ttgaaagtag atctttactg ttatctagaa tatggtgttt
     9301 cagttcctaa agtagcaatg gaaatgcagg aacgggtcag ccagcaagtc cttttcatga
     9361 cggatatcga tttatctgaa gtcaatatcc atgtagtagc tgttgttcct gaaaaattag
     9421 aacaaccaac aatcgaagaa ctttttgaag acgaagagga agagaatgaa taaagaatta
     9481 tcaagacacg agattcggga aatggcattg caggctttat ttccattaga cttcaatgca
     9541 gacctaacaa aagaagatgc gattttcaac gcaatcgaat tagaccaccg cgacatgatc
     9601 aacgaagatg aatcagagtt tgttcctgtt tatcttgata cgctagttgg cggtgtctgt
     9661 gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc
     9721 aatcggatct caaaaatgga tttggtgatc ttgcgtatcg cgatttttga aatgacttat
     9781 gtgacagatg taccagcggc tgttgcttta aatgaagcaa tcgagttggc aaaaacattt
     9841 agtgatgacc gttcacgtaa attcgtcaac ggtgttttat ccaatgtatt gaaagaatta
     9901 gaagctgggg cgtaagtcct agcttttttc tatgaatcat cataaacctg ttataaatcg
     9961 ggaaaattct atggtagaat gaaaacacaa aaaatatctg ggagtggtta gatggcagaa
    10021 ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag agattgctga aaaaatccaa
    10081 aaattgaaag ataatggtat ccatcccgga ttagtcgttt tattggtagg ggaaaatcca
    10141 gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa aagaaatcgg aatccattca
    10201 ttggtagagc gttatcctga gacgatttct gaagacgaac tacttgcaga aattgaaaaa
    10261 tacaatcaag atcctagttt tcatggtata ttggtccaat taccattgcc aaagcatata
    10321 gatgaagaaa agatcctgtt agctatccgc cctgaaaaag acgtagacgg ttttcatccg
    10381 atgaatttag gcagactcct tgcgggtaaa ccggacaaga ttccttgtac tccatacggc
    10441 atcatgaaaa tgtttgaagc ttattctatc gatccggcgg gtaaacgagc agtagttatc
    10501 ggacgaagca atatcgtcgg taaaccgatg gctcagctac tcttgatggc agatgcaact
    10561 gttactgttg ctcattctaa aaccgaaaat ctggctgaac tggcacaaga agcagatatt
    10621 ttagtcgtag caataggacg tggacatttt gtcacaaaag aattcgtaaa accaggtgct
    10681 gtagtgatag atgtagggat gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa
    10741 tttgatgaag tggagcctat tgcaggctgg atcactcctg tgccaaaggg tgtagggcca
    10801 atgactatca cgatgctgat gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt
    10861 gaatagatgg cacaagaata tttaactgtc actacattaa cgaagtattt gaaaagaaaa
    10921 tttgatgccg atccttattt agaacgcgta tatttaacag gcgaaatatc gaatttccgt
    10981 ttacgtgcca atgcccatca atatttcagc ttaaaagatg accatgcaaa aatatctgct
    11041 attatgttca aaggagcatt ccaaaaatta cgattccagc caaaagaagg aatgaaagtc
    11101 cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt cttatcaaat ctatgtagag
    11161 cacatggagc ctgatggaat aggtgcttta tatcaagaat tggaagaacg aaaagaaaag
    11221 ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga
    11281 atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg atatcatcac gacggtgaaa
    11341 agaagatatc caattgtcca attggttttg ttcccaacag ttgtacaagg aaaccaagca
    11401 gcggacaatg ttgtcaggaa tatccagaga gtagaagaga tgggcaattt cgatacaatg
    11461 atcatcggtc gtgggggtgg ttcgattgag gatttatggc cgtttaatga agagcgggtt
    11521 gctcgggcga tttatcaagc taatacgccg gtgatttcat ctgtaggaca tgaaacagat
    11581 accacgatcg ctgatttagt agcagatgtt cgggctgcca ctccaacagc agctgcagaa
    11641 ttagctgttc ctgttttgtc agaagagttg atgcgcattc aagaacggca agctagattg
    11701 cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt
    11761 tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc agaccatcaa attagaccag
    11821 ttgaaacagc gattatatca agctacacag caaatttatc atgcgaaaga aaaacaaacg
    11881 atcgcattaa gccaccaatt ggaacaagtg gctccgcttt atcgagttaa agcagctcgc
    11941 caagaaactg cttatctaga aaaacgctta gaagagaaaa tccaacaata tatgaaacaa
    12001 cagcagcagc gttttgctca agctgttcaa tctcttgatt tgttgagtcc attgaaaatc
    12061 atgggaagag gctacagcta tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag
    12121 attcatcaag gggatttgct gacgatccat tatcaagatg gaatcgttaa aggacaagta
    12181 caagagatag acaaaggaga ataaaaatat ggaaaatcca acttttgaag agtcactgca
    12241 agaattagaa aaaattgtca tgcagttaga acaaggagac gttccattag aatcagctct
    12301 agattcgttt aaacgaggaa tggaattaag caaacattgc caagatacat tagctaaagc
    12361 agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa
    12421 cgaggaaagc tgagatgaga ttaactgact tttcagctat tcatctacct gctgtagaaa
    12481 aagaaatctt gtctttttta gatgagcata caacggatag aggactctat gatgcgatga
    12541 gttattcagt gaaagcaggc gggaaaagaa tacgtccctt attactttta acggccgttg
    12601 cttcctttga tgagcctatt gatgttcccg tctatcaagt agcagctgct ttagaaatgg
    12661 tgcatacgta ttccttgatc cacgatgatt tgcctgcgat ggacaatgat gatcttcgta
    12721 gaggaaaacc taccaatcat aaagtattcg gcgaagcatt agctatattg gccggagatg
    12781 gtttgcttac aggagcattc cagctaatca gtatggcaca cttaggtaat tctcctaagc
    12841 ttctgctgct tcagcaatta gctgtttgtg cgggatcaca aggtatggtt gctgggcaag
    12901 ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga agaattagca tttattcatg
    12961 aaagaaagac agggcatttg attcgttatg ccttgctggc tggtggcata ttagcgaagc
    13021 aacctgaaga aatcttattg ctacttcaac gattagccga acatttaggt ttagcttttc
    13081 agataagaga tgatctactc gatgtgattg gtacaacgaa aacattaggg aaaacagctg
    13141 gtaaagatga acgtatggaa aaaaatactt acccgcgttt attaggccta gaaaagacac
    13201 gtgaggcgtt ggagattgag ctgttatccg ccaacaagat catagacaaa ttagaagagg
    13261 acgttttttc ttttgatggc gggctgatgc gccagatgat caagcaattt gatgtggaga
    13321 aatgagggaa agaatggaaa aagaacgcgt agatgtatta gcggtcaaac aaggattatt
    13381 tgaaacaaga gaacaagcca aacgttcagt gatggctggt ttgatttaca acgaaaaaaa
    13441 cgagcggttt gataaaccgg gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg
    13501 taaaaaactt ccttatgttt ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt
    13561 tgatctatct gttgaaggaa aaacattgtt agatattggt gcatccactg gcggatttac
    13621 tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct ttagacgttg gctacaatca
    13681 gctagcttgg aaaataagac aagatcctag agtcgtcgta atggaaagag tgaattttcg
    13741 ttatgcaaaa cccgaagact ttaccttagg aattccagaa gttgctgtta tcgatgtttc
    13801 ttttatttca ctaaaactga tgctgcctcc attacatgct attttaaaag aagagggaga
    13861 agtgattgct ttaatcaaac cccaattcga agcaggcaga gaagctgtag ggaaaaacgg
    13921 aattgttcgt gaccctcaga cccatcaaaa agttttagaa gacattttgt catttgcggc
    13981 acaaggtgga tatgatgtgc tggaattaag ttattctccg atcacggggg gagaaggaaa
    14041 tatcgagttc ttggctcact tgaggaaagt acccgaaagc ggaacaatca attcagctat
    14101 taatatggca gaagtggttt caaatgcaca cgaacagttt gaccataaat aaattgtaac
    14161 tgtgaaaatc aagtgagaag tatgcggatt cttctttatt ataccaagga aataagctat
    14221 cattgattaa gaagaatatg ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc
    14281 gtttgattac tcgattgttg agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa
    14341 tcctgaaaag caaaggaata tctgttactc aagctacgat ttccagagat ataaaggaac
    14401 tgaaactgat caaggtaccg gcttcagatg gcggctaccg ttatagcata ccagctgaaa
    14461 caaacgaaga tgtaaatact aaattggaaa aactattaaa agacgctttc gtggcagttg
    14521 accaaatgga aaaatttgtt attttgaaaa cattgccggg aaatgcttcg gcagcagcaa
    14581 atttgattga taaacgttac aagaaagaat tattttcgat tatcaatgat gacgataatg
    14641 ttttgatgat tacacgaact gaagaccagg cagttttatt gaagaaagac ttcctgcatt
    14701 acctttaagt tgaggagcga atgtgatgct acaagaaatc agtattacta actttgcgat
    14761 tattccggaa ttgcggcttt cttttcacga aggaatgacc gcgctgaccg gagagactgg
    14821 tgcgggtaaa tccattatta tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag
    14881 cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa
    14941 acaagaagga ttctctgaac tgatggttga attagggatc gaaacagatg aggacaatct
    15001 gattgttcga cgggatatgt ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat
    15061 tatcacatta gctaatctga gaagaatcgg cagttattta gtagatatcc aaggacaaaa
    15121 cgaacatcag gaactgctcc agcctgaatc acatcttgct ttattggatc gttttggaga
    15181 tgtagctttc caacagaaga aaaagagtta tcagcaagaa tatatctctt atcgtgagct
    15241 ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat
    15301 gctccatttc cagcaagaag aaatagctgc tgctgatttg caagtcggag aagaagagaa
    15361 attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa attgctgatg gattagctgc
    15421 aggttacgga gcactgacga ataatgaaca aaacagtgta gatggtgttg gcttggccgt
    15481 ttcagaactg caagggatcg cccatctaga tgtggagtat gaagcaattt atgaaaatat
    15541 ccaaagtgct tactatttgc tgcaagatgc aatcggcgat atgagtcgtc agattgattt
    15601 attggagtta gatgagagtc gtttagaaga agttacgcaa cgtttagaat taatccgaca
    15661 gttgaaacgt aaatacggcg aatccattga gtcaatattg gcttactatg acgaaatcac
    15721 cgaagagcta gcttcttctg atttttcaga aggtcaattg gacaaaatga aagaggaact
    15781 agagcaaaaa gagcttctac ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa
    15841 aatcgcaaaa gaattggaaa aatccattct gcatgaattg aaaagtctgt atatggaaaa
    15901 cactgagttt gaggtacgtt tcttaaaaga agaaaataga caattgaatc atgatggatt
    15961 tgaccaaatc gaattttaca ttacgaccaa tccaggagag ccactgaagc ctttggtgaa
    16021 agtcgcttct ggtggcgaac tgtctcggat gttgcttgct ttgaaaacta ttttttcttc
    16081 tgagcaaggt gtgacaagta ttatttttga tgaagtggat acaggtgtga gcggaagagt
    16141 tgcacaggcc atcgcagata aaatttctca aatatcgaaa tactctcaag ttttgtgcat
    16201 cactcatctg cctcaagttg ctgcagtcgc cgactatcaa tattatattg tgaaagcagt
    16261 gattgatgga cgtacgcaaa cttctgtttc agagttgaaa acaaaagaaa gagaagaaga
    16321 aattgcacgt atgctggctg gaagtgagat tacaaaactc acactcgaac atgcaaaaga
    16381 actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag tggttcaggt ccttttcagc
    16441 agctgatagc tatattttat ttttacatga cactcattac agcagtcaat acaacagatc
    16501 caatcgttaa aatcagcatt gtccaaatga caaattttgt tactttagca aaagtacttg
    16561 tttgtttttt ttcacgcatt gttttcaacc tcgtcattaa ttagttgatt caagtgtaca
    16621 atcttttttc catttccaca agagggcttt tttataaaaa cttccctttt tttaaataat
    16681 aggtagttac gatacaaata aataatgtta atagaactag ccatataaag gcatcatccc
    16741 tttcagcaat aggtaactta atgttcattc catagatact gccgacgatt gtcggtatag
    16801 ttaaaataat cgttaaagaa gttaaaattt tcataatatt attcagatta ttcgatacga
    16861 ttgctgaaaa agtctgattc atttgttcta ctagttttaa ttgaatgcga gaagtagtca
    16921 ttgcctgctc tgtttccacc aatacatcac gtaaatgatt tttcaaagca tgattttctt
    16981 ggaaaagttt attttgcgct agttctttca atgcatctaa attagcagaa gtcgcttctc
    17041 tgaaataaac gaggcttttt tggatatcca tgatttgata aagttgtttg ttttcagtgg
    17101 atacttgaag tttggcttcc aaagtatcgc attgagattg aagcgtagct aaataactgt
    17161 tgtaagagag tgtgacctgc caaatgaatt gaaagaagat attgagatta ggcgaaatat
    17221 cactttttgg aaaggtctgg ctaaagactt tctttaaaaa cagtggttca tgattcgtaa
    17281 ttgtgataag cttctttttg ggcgtcaaaa taatagagag cgggtatgta tccacttgaa
    17341 aataaccgct aggacttgtt ttagcaaaag gatattgtaa caataataac gctggctttg
    17401 tcaactttga ttgattgaat tcttctgtac gggaattttc tgcatcgtcc aatatggaag
    17461 tgatgtaatc tgacggaatt tcaaattgat gtgtcagctg atgaatctct tcttgattag
    17521 gacgttctac acaataccag atcgtttggt cagtttcctt tccggtatta cgcagtttct
    17581 cattatctaa tgtaaaatat ttgatcatga ttttcctcct gttgcctgtt tcttttatta
    17641 taacggatag ggaattggaa tacatggaat gtgtttgtaa gatatagaat ataactgcat
    17701 aaatttaaat aaaatgaaac agttgtgtaa agtatataca gtatacttga agcaacgata
    17761 aaaaaggagt ttacgttcat gggcacattc acaccactta tcttgttttg gtatatttac
    17821 ccggtaatcg ttttgtttgg ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt
    17881 tttaaaataa aagcccctga tttggcgatt ccttttttgt ggggcggtat gcatgcattg
    17941 tctagaaata cagggacaat ttctatctta ccgtttttct ttatttctgt tttgctaatg
    18001 ggaatttgta ttgctgtttt ccaagcatat tattatgaag aaattattta tccgcgttat
    18061 ttcaagatga cttggcgttt ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc
    18121 attgtgaaca tactttctta cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa
    18181 aggctttttg cagcttgatt tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga
    18241 tttcccagaa aaggtggtag aaagtggggg attgtggtag actaatttta gttagtggag
    18301 gaatgggggg gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac
    18361 gtttaatcgt gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg
    18421 gattggatgg ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac
    18481 tgaacgaaat gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg
    18541 ctgccactga atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa
    18601 attatgcagc tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt
    18661 gggatgagac acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg
    18721 ctgaaagtat gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac
    18781 atttcaacac tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga
    18841 tggcgtttat gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca
    18901 actaagttct aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc
    18961 caagctgcgt ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt
    19021 cagagaatta gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga
    19081 tctaggggtt tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga
    19141 tgcaccgttg gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa
    19201 tgaatattcc taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc
    19261 caaacaaatt gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg
    19321 tgagcttgtc gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca
    19381 tcctgcaaaa agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga
    19441 agaggcctct ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat
    19501 cacattccat tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt
    19561 acaagatcta ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt
    19621 cattactcgt aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg
    19681 cagtgcaaag ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag
    19741 aattaaagaa aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac
    19801 aacaaatgcc tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac
    19861 gattaaagca gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg
    19921 ttgctttatg tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa
    19981 tcacgtcaat acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag
    20041 aaaaaaacga actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt
    20101 ctattaatga cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc
    20161 ggtttatgga aaagaaaaat ctaaatccga taaacaatcg caaaaaagta ggtatcattt
    20221 tatttgctac gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta
    20281 ttggaggtca tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa
    20341 gtgaagtagt aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag
    20401 aagatgcctc ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata
    20461 aaaaactgta tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc
    20521 tttctataga caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt
    20581 atcaagttga atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg
    20641 aagcggacat gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga
    20701 tgtatccaaa tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg
    20761 agaatggctt agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta
    20821 aagatggtaa gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg
    20881 cagaagaaga aaaagctgtc gacggacaag atatctacac gactttagac agcagactac
    20941 aaagttattt agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga
    21001 cagcagtcct gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt
    21061 tcaatcctga aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg
    21121 tacaagatag ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg
    21181 aagaaggaga attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg
    21241 ctacgatcaa tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt
    21301 catggtcaag taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata
    21361 actatttaca aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa
    21421 acggtgcttt acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg
    21481 taggtgtgac gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa
    21541 tgattcaacc tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa
    21601 ctcaaacaga agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat
    21661 atatgagaga tgttgtcgaa agcgaaaact acggtagtgc ttatggtgta tatagtgtac
    21721 ctggatacaa tgtatcggca aaaaccggaa cagcccaaat cgcttctgat acagggggat
    21781 atcaaacagg agatacagcc tacctgtatt cgattgtgga gatggttcct tctgaagatc
    21841 cagactatgt cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg
    21901 ctaaaatcgc aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt
    21961 cagatacaga aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg
    22021 acgttgcagc agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta
    22081 aaaaagtaca aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga
    22141 tcctatatac tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata
    22201 tcatgaagct ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg
    22261 tcaaacaaga acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag
    22321 aagaataaga agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc
    22381 attgtcagca gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa
    22441 atgaaaaagc aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc
    22501 ggaactccta caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg
    22561 gttggagctt ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg
    22621 ttatacggag taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg
    22681 ggactgaact cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt
    22741 gtttaccgtt ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta
    22801 ggaatcattt atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat
    22861 ctgacagatg gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac
    22921 ggtctaattg cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta
    22981 ggaggtttac tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat
    23041 gtcggttcat tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa
    23101 tggacattgc ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa
    23161 gttacttcct ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac
    23221 tttgaaatgt gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt
    23281 gtgacttcgt tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat
    23341 atcaacatat gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc
    23401 ggccaaatta ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga
    23461 cgaaaatccg gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca
    23521 tccaatcgaa ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta
    23581 cagtcatcct ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga
    23641 gctagcttac gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac
    23701 aacgacaaca acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg
    23761 tttagcgggt aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga
    23821 taaaattgtg atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca
    23881 tatagcggtg attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga
    23941 atatgtcaaa gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct
    24001 taactggaat cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc
    24061 tttctcgaca aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta
    24121 taaaaaagaa aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga
    24181 aaatgcacta gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg
    24241 agaaacgttg cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca
    24301 aggcagaaaa ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct
    24361 aagtggattt gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac
    24421 atttgatgaa ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac
    24481 aaaagaaaag ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga
    24541 ttctgtagaa caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt
    24601 attatcacca gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag
    24661 attcatggaa gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga
    24721 aaattttggt tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca
    24781 attatgtaaa gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc
    24841 tagaaaacaa aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat
    24901 ttaaacgaaa aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc
    24961 gtcaagcaaa aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat
    25021 atgtttccgg agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg
    25081 aacaaaacag tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg
    25141 ctctttcatt taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta
    25201 atcctcgagc acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt
    25261 tggatccaga aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca
    25321 atcaagcagt atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg
    25381 cttcaggaga tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata
    25441 ttagtgtcca gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg
    25501 tggggcgtgc tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt
    25561 tggttcccag cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc
    25621 acgcaggagc agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga
    25681 cagtcaacga aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag
    25741 aacaaggaat ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa
    25801 aataaaggcg gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc
    25861 ttctgatgaa aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca
    25921 agagatgaaa aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga
    25981 ttctgaagaa gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa
    26041 aatcgaattt tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt
    26101 tttgaaccgg ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc
    26161 tttgatcatc actgtattgg gtataccgct gatttttttg atctattatg tttctcctta
    26221 tagcaaatta caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat
    26281 ctctgataca aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac
    26341 ttatatcgat cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa
    26401 agggatgaat gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa
    26461 aaacaatcaa tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa
    26521 tcctacaaaa aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt
    26581 aacaaagcaa tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta
    26641 cacgccaaga gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt
    26701 tattgtaaat atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga
    26761 tatggatgaa aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag
    26821 cactcaagaa acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc
    26881 ggatcaaaca aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc
    26941 gtctagcaca aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga
    27001 taatccaagt acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta
    27061 agaatgtact ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta
    27121 ttaataagta tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag
    27181 gagggaatcc cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca
    27241 tctgttaaag ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga
    27301 aacgcaaaat cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa
    27361 gcgatacaac gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta
    27421 agtgtcggtt tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt
    27481 aacggagatt caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt
    27541 gttcgttcaa tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc
    27601 gatgggttcg agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac
    27661 ggtctgttgt ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat
    27721 gccggattaa tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt
    27781 tctgacggag aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca
    27841 acggctgtta tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa
    27901 tttgtcacta aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg
    27961 aaaataaact acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta
    28021 gatgtgattg gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt
    28081 tcagctcgaa tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca
    28141 ttagagttgc caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg
    28201 gacttggcac aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg
    28261 ctgagaaacc cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt
    28321 gaagtgtatc aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg
    28381 gtagttgaac aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc
    28441 tacgatgagc cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc
    28501 tttacaaata tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga
    28561 taataatatc aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa
    28621 tgctgtaaac cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac
    28681 agacgtgcaa gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata
    28741 tactcgtggt ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag
    28801 tgaacaatcc ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat
    28861 gggtggagga accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg
    28921 cgcattgact gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg
    28981 ttttgctgca gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc
    29041 aaataaccgt ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga
    29101 agcagataat gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg
    29161 ttacgttaac cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt
    29221 gatggggatc ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat
    29281 ctcctctcca ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac
    29341 aggcggtttg gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc
    29401 agcaactggc gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga
    29461 aatccgtgtg acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag
    29521 ccgaccagct agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga
    29581 tcaagctaaa ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg
    29641 tgaacaaaat gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga
    29701 atttgataca ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc
    29761 accgtttttc cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc
    29821 agaaaatcaa gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag
    29881 ttacattagt agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag
    29941 ctggcgttat gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt
    30001 tatttgattt tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa
    30061 aattgattgt aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag
    30121 aaatccagaa gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg
    30181 aggaatcaaa acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg
    30241 cctgcacaaa aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg
    30301 agcaaatcca taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag
    30361 gttatgccca tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg
    30421 cgattgaaga aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa
    30481 ggaggaacaa aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct
    30541 gatgaggaag agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca
    30601 actaaaaaaa atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat
    30661 gaaaaacaaa aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa
    30721 aagaaaaaag taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca
    30781 gcttcaagaa cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa
    30841 ccaagagctt attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc
    30901 ttagtgaatt ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca
    30961 ggaacagttt atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt
    31021 acaccgactg ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg
    31081 tttgatctgt aagaggagga actacgattt atactattgc actattactt catttacttc
    31141 aacgggcagc ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc
    31201 caggcgggta tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca
    31261 gtttgttcga tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa
    31321 tcctgatctt gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc
    31381 atttagttta ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa
    31441 ttttggatgc aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg
    31501 taggcgattg gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg
    31561 acccaagaca atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca
    31621 tgttttatgg cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt
    31681 acgaacctgt agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat
    31741 ttgccagcct aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct
    31801 cttactttgg cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg
    31861 tggatcattt tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac
    31921 caatcaaata cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag
    31981 ttagttccat gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt
    32041 ccaagcagct gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg
    32101 attttgtact tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc
    32161 aagaacttga ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta
    32221 aataaaataa aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac
    32281 attcccgaca aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt
    32341 tgttcgtgac tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca
    32401 ttctgaagaa aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat
    32461 cgtagctcaa gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat
    32521 cgttacttct gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca
    32581 tcaattaaca actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa
    32641 agcaagacag cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca
    32701 acgtatcagc ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga
    32761 aattttacaa ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt
    32821 agcaaaacag cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga
    32881 agcacctatg acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa
    32941 tgaaaacgag tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga
    33001 agttcggtaa gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt
    33061 agagtaagaa gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg
    33121 caactaaatt tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg
    33181 tgtccggaaa acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt
    33241 aggaaaaaca aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa
    33301 agaatgggaa gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag
    33361 ctttgttctt catgatggac ctccatatgc caatggtaat atccatttag gacattcatt
    33421 gaataaaatc agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc
    33481 atatgttcca ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg
    33541 cgtgaaacgt aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact
    33601 ttcccaagta gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga
    33661 acatccttat gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa
    33721 aatggctgaa aaaggctata tttacaaagg attgaaacca atttactggt ctccgtcaag
    33781 tgagtcttct ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt
    33841 tgtagctttc aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat
    33901 ttggacgaca acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt
    33961 tacgtatgta ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga
    34021 aacagtccaa tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca
    34081 agaaatggaa tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt
    34141 aggcgatcat gttactttag acgctggtac tggattagtc catactgctc caggacatgg
    34201 ggaagatgac tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc
    34261 aaaaggattg tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa
    34321 tcctatgatc actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac
    34381 tcatagttat cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca
    34441 atggtttgca tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga
    34501 gtgggttatc ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg
    34561 ggtaatttcc cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg
    34621 agaagcaatc atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg
    34681 atcaattatc tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc
    34741 aggttctcct aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc
    34801 tggttcttct catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat
    34861 gtatttagaa ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt
    34921 ggctatcaat ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg
    34981 tgaaggccgt aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa
    35041 acaatttggt gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt
    35101 tcgtgtttct atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac
    35161 gatgcgtttc ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta
    35221 caatgattta cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga
    35281 aatccgagaa gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa
    35341 cttcttgacg gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat
    35401 tgaagcagaa gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt
    35461 agctttgaca aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt
    35521 cctgaaagaa gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc
    35581 aaaccaagaa gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt
    35641 aaaagcattg gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact
    35701 aactgtttat ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc
    35761 acaattattg atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc
    35821 agataacgct atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac
    35881 ttgcgatcgc tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct
    35941 atgcggtcgt tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt
    36001 cgaagaaaag taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt
    36061 atcaatcgtt taattttcca cgttcacgat accagttatc tggttgccag atccattcga
    36121 atccgtcttt ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt
    36181 tgggaaaatc cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag
    36241 attgagcgac ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata
    36301 gtaatttttc gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca
    36361 atttgattgg ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa
    36421 atccttcagt tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac
    36481 atggttcatc gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga
    36541 tccgagtccc tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc
    36601 aacggaagtt atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat
    36661 ccactttgtc ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat
    36721 attgcccgcg aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca
    36781 atgctttgat cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg
    36841 caagtaatgc tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat
    36901 gatcgtagta gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat
    36961 tgtcgatata gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg
    37021 agatgttttg gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg
    37081 ggaatacttc tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg
    37141 gtttttctat gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat
    37201 gagaaacgat cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt
    37261 ttaattgata gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg
    37321 aatcatttac atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat
    37381 ctgaaggatt caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc
    37441 agggacggcg agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa
    37501 ggcggaacaa agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg
    37561 taaacaatac atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa
    37621 aatcgtgctg gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat
    37681 cggtccttca taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat
    37741 agtgactaag taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt
    37801 accgacagga taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt
    37861 aggaatcgat cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc
    37921 tgcaagatgt tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca
    37981 gctatagtct aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact
    38041 gctagctttt tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac
    38101 aagctcttct ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag
    38161 actagatacg atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat
    38221 tttcaaataa tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca
    38281 tcattagtaa gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag
    38341 gatttttaga cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt
    38401 ttataaagga tatatatata cattgggagg gatctatttt tcagataaca acttatatga
    38461 agtgtctgca gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc
    38521 atactgatca cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga
    38581 ctgaccgttg atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct
    38641 atcactaaag aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt
    38701 tttctttttc aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat
    38761 catttttttt atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt
    38821 cttctttatt tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt
    38881 ttttactgtt tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt
    38941 tgaagtatca gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac
    39001 attgtatgaa aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa
    39061 aagaggtcct gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga
    39121 aaaccgccta tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc
    39181 aattgcctaa tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat
    39241 taactggtcc atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata
    39301 tttatttaga gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg
    39361 aaaataaagc gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag
    39421 gagaattaac gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt
    39481 tcgaacatct tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg
    39541 ttcgacatgc gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag
    39601 atgaggtaac tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta
    39661 tacaatagaa agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta
    39721 ctcatcaatt tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt
    39781 ctagacattt ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg
    39841 caacagattt gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc
    39901 cgaatagtct tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag
    39961 ttgaaaagcc agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg
    40021 aacacaatgt gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga
    40081 ttgccgatta cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat
    40141 attcttcgcg ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt
    40201 tctcaggtgg tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt
    40261 ttggaatgcc aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc
    40321 ttggatgggg gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg
    40381 gtgattctta tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag
    40441 tcaatgcaat tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg
    40501 cggtaacagc tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg
    40561 ctcatccgaa agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa
    40621 atgtgaacca agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata
    40681 atgaggaaga tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa
    40741 atatgaggat aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact
    40801 aagtaataaa caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta
    40861 tctttggcct ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc
    40921 ttcttctcaa gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa
    40981 agagttgaaa gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt
    41041 gaaggaaaaa ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc
    41101 aaaaaaaata aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt
    41161 tgacgctggg aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca
    41221 gctagctttt ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg
    41281 gaaaacgttt gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca
    41341 ttacttttta cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat
    41401 agaagatttt catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga
    41461 aaaattgctg tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact
    41521 ccgaaaagca gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga
    41581 tgtggcggct agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc
    41641 gttaagtgaa gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg
    41701 aatagtaatt acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc
    41761 atcaaatata acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc
    41821 aaccaaagaa gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa
    41881 ggagcagaac ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa
    41941 aaaagagaga aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa
    42001 aacgggaaca aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc
    42061 ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta
    42121 actatcgatc gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt
    42181 gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat
    42241 gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat
    42301 tctttaaaag tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg
    42361 catacgatta tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat
    42421 cattttgcaa tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt
    42481 gcggattatg ctcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg
    42541 gcttacacag gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata
    42601 ttgtttttag atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc
    42661 attgataaag gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt
    42721 cagatcattg gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg
    42781 attcctatgg caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa
    42841 atcatctctc tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa
    42901 aaagaagcga ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa
    42961 cgagacttga aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa
    43021 aagctaatca ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc
    43081 aaggaaatac cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt
    43141 gaaccaggat ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat
    43201 aacgatattg aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta
    43261 gagaccctca tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact
    43321 aaaagttcga ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg
    43381 tatgatgtgg ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt
    43441 gctttgcatc tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca
    43501 gatttgaata acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca
    43561 acgatcattg aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt
    43621 atgtttcttt ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt
    43681 ctcatattga tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta
    43741 ttaggaacaa aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg
    43801 tatgaagaag tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc
    43861 ttgttaacag atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc
    43921 attcggacga aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg
    43981 gcaagtgttg gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg
    44041 atcgtacgtg atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta
    44101 atcgtgacat gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca
    44161 gtgattaatc aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc
    44221 aaaaaacaca tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt
    44281 gatgttgatt atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa
    44341 ctaaatattt taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca
    44401 cttgctggat cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag
    44461 acagttcagc agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga
    44521 atcgttatcc atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa
    44581 tttgagaaac tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca
    44641 aacttgatgg cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt
    44701 cttacacaaa tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta
    44761 cacactaaac atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt
    44821 cagctctttt ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg
    44881 agtgttaaaa atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag
    44941 tttattggtc acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt
    45001 tgcggtctct gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt
    45061 attaggtcct caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg
    45121 tattccatta gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct
    45181 tgcacaagca gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg
    45241 gaagatcaac aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag
    45301 agcgatgcaa tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg
    45361 aaaatcgctg atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg
    45421 acacaagaag cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat
    45481 cacttaatga catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt
    45541 cgcgtataca gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac
    45601 ggatttttta ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt
    45661 aaaaaatgaa cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa
    45721 tcggagcgca aaaacattta gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa
    45781 caatggcggg atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt
    45841 ttaacagtac ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg
    45901 tcatcatcgg aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct
    45961 ttgccttttc ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg
    46021 ggatcgttgg tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag
    46081 cattagatgt gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg
    46141 caacgattgc agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt
    46201 tgaatggaaa cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt
    46261 gtaaattgat gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat
    46321 ctaaagcatt tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca
    46381 attatatcgt aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata
    46441 ttgcagaacc attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat
    46501 ttgttcaagt attctggttc tttggtattc atggaccaaa tgtattggca ccagtgctag
    46561 agggaatctg gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg
    46621 gaaaaactgg tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg
    46681 tccgcggatc gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac
    46741 tgatagctat cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca
    46801 ttgggcctgg tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga
    46861 atgcaatcat gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga
    46921 cagctactta tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc
    46981 cgttcttatt gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag
    47041 tatgtatggt cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg
    47101 accctgccgc aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg
    47161 ttcctcataa tgaattttct aggaggaact gaaatgatta caattgccag tgcaatgcaa
    47221 caagatgaaa tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt
    47281 aaagaaaaac aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg
    47341 gctaaaaaag caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa
    47401 tcaacagctg tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt
    47461 ttatttttat gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa
    47521 aggtatgatt tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc
    47581 ttcatgaagg ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga
    47641 aaaaattaaa tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg
    47701 atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg
    47761 tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc
    47821 agtcttataa gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca
    47881 aaccattgga cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca
    47941 tgcttcaaaa gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac
    48001 gtttcaaaaa aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact
    48061 atttagttat tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt
    48121 cctgcgaatc ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag
    48181 agagctaggt gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg
    48241 ggcagataca cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta
    48301 cggatttttt gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt
    48361 gaatttttac ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga
    48421 agcaagggga caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga
    48481 tcgtaaagta agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg
    48541 ctatgaacaa tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat
    48601 caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag
    48661 aagaatttat gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg
    48721 gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa
    48781 tgaggaagca acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga
    48841 gtggggattt ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa
    48901 tggtgctttt gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct
    48961 attagaaaat ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga
    49021 tatttctttg tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca
    49081 aatagaagaa gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa
    49141 tggaaaaaat catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat
    49201 ccaacatatc acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga
    49261 aagaatacag gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca
    49321 gccaattctt aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac
    49381 ccttttgttt ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta
    49441 aatttgaatt ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct
    49501 tctttcgtaa tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt
    49561 tcttttggct tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt
    49621 ttatcatctg ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag
    49681 cagataagct tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg
    49741 tagctcagta ggatagagca atcgcctcct aagcgatagg tcgctggttc aattccagtc
    49801 aggaacgtat aaataacccc tgtatccttt gcggtacagg ggtttgtttt gtatttgtgc
    49861 caaaaatgtg ccaaactagt tatcattttt atataaacta tcgaacacag tattgatttt
    49921 ttctttatcc tgatcttcaa gctctcgaac aatatgaaga taagtagaca ttgtagtttc
    49981 taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa
    50041 aatagatgca tgtgtatgcc ttagtccatg caaagtaaat tgcttttcaa tccctaattc
    50101 ttttaatttt ttgcgcaaat atttgctgac ggcgtttgat gaaactaatc cattttttaa
    50161 gttgaaaaaa acaaaattat gagggttttt gatttcaaaa ttctcgaata attctttttg
    50221 attgatttta aactttttta agagatcaat cgtatgttta tcaatggaaa ttttcctatt
    50281 tgaagtttcg ttttttgttt ttccccattc atttaattta taattccatg ttttattaat
    50341 agtgatgatt tgatcttcga aatcgatatc ttcccatgtt aatcctagaa gttcagcaaa
    50401 tcgcattcca gtggcaccag caactaacac gagcatagga gaagagtact gcgcagaaag
    50461 gttttcttct acgagtttca ttaaagattt aaattgatca taatctaaat atttatcttc
    50521 ttttggcttc aatgaatctt ttcctttgat tactgctttt ctagttggat caaacggaat
    50581 taatccttct tctacggcat ctttcaacga cgctctaata tggttattaa acttaataac
    50641 ggtagatttt acatggtctt tagcatactt atttaaaaag cgttgatatc ccactctatc
    50701 taaatcagaa atcaataccg ctggcatata tttctttatg ttcattaacg tatcttcgta
    50761 ttttctataa gtgataggag atactgttcc ttctttataa agttgcatcc aatcctcaaa
    50821 gtaatctgat aataatagat ttttgcgatc ccctttaagg cctttgttca agttatattc
    50881 taattcgttg gcggcatctt ttgcttctcc ttttgtctta aatcctgatt ttcttatctt
    50941 cgtgtatttt ccgtcatctt ttttgtatga aatttcgtat tgccaactat ttccacgttt
    51001 gactaatctt gccataattg aatcagctct ctttctctga tacaatagga actataaaga
    51061 agcccattgt ttgggtttta ttattttttg cacatccttt ttgcttgccg gcgcaaggat
    51121 gtgctatttg tcgttttaat ggtataattg gatgtgtatt agatcatcca atcagaggtg
    51181 tttattatga ttaaagtgat ttttaaaaga ataactgaac ttccagagtt tagattattt
    51241 gttgattatt ggtatgtctg gttttttatt gttgcattgc tgataggtct agatgtcata
    51301 ttagaaagaa aacttaagta agagttgagt taataaattg aattcctaaa tgagatattt
    51361 tgtagcccat attttgtttg ccgacatcac ctaatactat ggagctaatc gttttttcta
    51421 acagaccaaa atcagataat ctatttaagc taccatctat tagctcttgt gaatagccag
    51481 acatgctgat atttccagtg aaattttcgt ttccacgact cattaagcat gctaacaatt
    51541 gaatctcaat cgatgttaat tgattcagta caagaagata atactcctcg ttatcccatg
    51601 aagcgctgtt aattcgaagt aaacaattct tataaagatt tttatacata tctattttat
    51661 tttgtgcctt tgatttttct acctcgtcat ttatcccttc gaatattcca atgagttggt
    51721 ccttgttctc taagttgaaa acatcttctg gaatttgatc gcgaatgttt tctaatgatt
    51781 cgtttaaatt tttgtagaat ttctcgatgc gtttgaaacg cttctcgttt ttagacccaa
    51841 aataaagtga ctgcacaacg ccaccaatat aaggaattgc tccaataccg ccttcaacta
    51901 ttaattcaag atattctttt ttggttagtt tttcattgct attatccata gttacctcct
    51961 attttgtcat ttcctttaca agcattgtga ctcctgatgg cactcttttg tataggtttt
    52021 attattgtgc acaatctaac tcttagtggg gcgtggttca tttatttttt acttacttta
    52081 ctattaatca tatcccattg agcataggat ttttctatgt tcgatcctct tgaactaatt
    52141 tctcctgtaa aagttacttt atcgcccttt gtaaaggtat cagcattgtt taagtccttc
    52201 gcaaaaatta catatgaaac tttaggtgtt gcagaaatat cattccaact tttatcatcg
    52261 tactctttgt ctgctataat ggctattcta gtagtcattg attcgataac agttccttca
    52321 aaagtatatg ttttaccact aatatattta gtaaaagctg tattttgctc agtagctgga
    52381 gtaatagaat aataactatc taagaaagca ccccagtcat gattatttgt ctctaaaaat
    52441 tcttcaaatt gagttaggct tttattgctg ctgttagatg tagtagtttg agtatcagat
    52501 gatgagcttt cctgtttgtt attgtcattt gcctcgtttg atgaggaaag cgagaaacct
    52561 acggtagtaa aaataatccc gataataagg agaatcatag gtgttttctt atttgttttt
    52621 ttgataaatc ttacaattaa catgattaaa cctataataa ataaaacaat accagtaaaa
    52681 ccaaatatac cgtccattaa tagatcctct ttctcttttg atattgaata ttataaacca
    52741 agcaattctt ttttcttaaa atcgaattct tcttgattaa taatcccttc atctaacaac
    52801 tctttatact ttttaatttc atctgcactt gaaatttgtg tttctttgga attattatct
    52861 aatatagatt gttgcttctc tatatatttt tttaaatttt ccatttcatt ccaatatttt
    52921 tttgtaaaca taacggtgtt ttcatctttc gtggccgcta atatacctcc gcgactttcg
    52981 tttccgccta gcaaggtaaa ttgtatataa ccattactca tacctggttt ttttaattgt
    53041 acggctgata tatttttaaa ggggatagtt ttttctcctt taataccttg attaactaaa
    53101 tttataaatc cttttctaat aattgaaata gaatcatcag ttactttaat caaggttttt
    53161 cctggagatt ttattaaaat ctctttttcc attttttctt cctcatttct gtgatattat
    53221 tttttttgta gaagctcata tatgaggaaa gagcccgtgt tgcagcacgg gcttttttaa
    53281 tgttttgctg aaatcggttt ttttaataat tcttgatgta ttcatacata ttgccttgcg
    53341 taagaatatt ttttcttaaa atagcattgg cagacagcat aacaagtgta tctgagctta
    53401 ttctgaatag aataatactc cataaatttt tcgagattaa actgagattc atcagtcagt
    53461 tcgttctcaa tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca
    53521 aattttgagt gaaaagttgt agaagtatcg tacaaaactg aaaattcaaa atgagaggcg
    53581 ctgaaatgtg caagttcatg agataaatga aaggcttctg cagtttcacc gtatagattt
    53641 tcattcaaaa aaatgattct gggttttgga tagtagaaac caggctcttt catctccata
    53701 taaactactt tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaacc
    53761 atcactccaa ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg
    53821 tctccttaga tatttctttt ccatcaaaag aaaaaacagt atcgtcttct gataaatcca
    53881 catgtttagg ggtttctctt tcttctctac ctagaaggta gtctacagag acatcgaaat
    53941 agtcagcaat ccgacttaat tcatctgaat taggagtatt atttttccac tttgctagat
    54001 aaccgttaga atatccaaaa ttaatttcta actgccgtat agatatcttt ttcctcttcg
    54061 ccaagtcttt tattatttca taggtattca tcgataaaac aacctttcta aatgtttaca
    54121 aaaaaagttt agaaaaatac gcagaaataa tttgactaat tctgagtaat gagctatact
    54181 atatcttgta aacagattta acaactaaaa agacaacaaa aaacactatt gatatgtaaa
    54241 tgcagaccgc caagaaagct ttaaaatcaa tgtttttatg tcttatttaa ttatgctctt
    54301 agtatagagt attactcaga tactgtcaat tgaatttaga aaaaagttgt taaatttgtt
    54361 tacgaatata aaagaaagga atgagaaata tgaacacacc gcaaattttt aatttcgaac
    54421 aaaacgaagt tcggacaatt ttagtaaatg atgaaccata ttttgtaggc aaagacgttg
    54481 cgagtgtttt gggttattca aacactaaag atgctttgtc gcggcatgta gatttggaag
    54541 ataagatggg gtcgcgaatc acgacctcag gtcaatcaag agagatgaca atcatcaacg
    54601 aatctggttt gtacagttta atcttaaaat caaaacttcc ctctgccaaa aaatttaaac
    54661 gttgggtaac aagtgaagtg ttgccagcaa ttagaaaaca tggaggttat ctaactccag
    54721 aaaaagtaga agaagctttg cttaatccag atacaatcat tcaattagca actcaactaa
    54781 aagaagaaag aactggaaga ttaatcgcag aacaaaagat tgcagagtac gaacctaaaa
    54841 tctcctattt agatagcata ttatcttcta cagattcagt aacaattagt cagattgcag
    54901 cagattatgg gatgtctcca caacagatga ataaattact tcataaacta ggtattcaga
    54961 aaaaagtcgg taaccaatgg ttattgtgca aaaaacacat gaaccaagga tacacaaaat
    55021 ctcatacaac tgagatcccg aaagccgatg gtggcactaa aattgtaatg aataccaaat
    55081 ggacacagaa agggcgtcta tttatctacg aattactaaa aaaagaagga tattaccctc
    55141 aaatggattt agaggaaatt ggttagaaag gagttttagt atgactgaca ttgcagaaat
    55201 cactcaacga gatagagaaa aaatcaaaga atatgtcgaa agttcaaagt tcttaactta
    55261 caccatgctt gctgaaagat ttggaattag caaaagctac ttatctttaa ttttaaacgg
    55321 taaaaagact tctgcagaag caaacagaat tatagattcg attatcacta tgtacgaatt
    55381 gtaaagggag gaacagctaa tgcaatatct agaagcaaaa atcccaattc cagaaggcta
    55441 tgtaattatc tcccaagtgg attatgagga gttaaaaaaa gctgatgata ctggtagatg
    55501 gatgacgttg ccagaagtgc tagaacggat taacagaaaa tatgattggt ttacttctag
    55561 agttttaaag aacccaagat atagaaatat tatcgatatc gagaaaaaca aaaatggatt
    55621 tgtctattat ccagttgaag gaagagacac atatctattt ttaagaagta aaacacttga
    55681 atttttagaa acaaattttt cggaaatctt aaggaggcaa gcggatggga aaatttaata
    55741 gagcgctagt gttcagcgca ccgctaatca tctacgcttt aggactttgg ggaagcaggc
    55801 aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg
    55861 aagctgagta cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga
    55921 gaaactagaa tgtatattac tattactcag tttaattctt tcactaattt ctctattggg
    55981 aagtttgtat ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc
    56041 atttttgttt ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg
    56101 taacaagata tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta
    56161 ttatcatcga ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg
    56221 cggtttaagt tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag
    56281 ttagaaatga ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca
    56341 acttctagtt gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata
    56401 aaacttatta ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc
    56461 accagttttt aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
    56521 tttgaaaaat aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa
    56581 acatcaacat gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca
    56641 gatgtttgta gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat
    56701 tagtagagat ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt
    56761 caaacaaaaa tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt
    56821 tcctcaaaca attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa
    56881 agatgtttcc atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa
    56941 gcaaaaagga aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga
    57001 agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat
    57061 cccaagatat ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat
    57121 gtggttgcca gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact
    57181 ttttggtgat gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt
    57241 gctggtggaa atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag
    57301 catggcaaca aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat
    57361 cataaggaga ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga
    57421 tcctgattgg ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct
    57481 tttgacgaat gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg
    57541 ggatcatgtt tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt
    57601 catcgaagat ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat
    57661 ctcttattac ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg
    57721 aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca
    57781 agaaaaaagg aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga
    57841 atgatccaca ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa
    57901 ctcatcaaga ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt
    57961 atgtatctgt ctcagtaacg gttaaaggac acacggaaac cgaattattt cctgttttag
    58021 attatcgaaa ccaaccagtt gttaaaccaa atgcaatgca aatcaataac tcattgaagc
    58081 gatgctttgt gaaagcattg gctctacacg gactgggatt atatgtattt caaggggaag
    58141 atattccaac accacctaga atcgatacaa agaaattaaa catgctagag acgattctag
    58201 aagctttcaa tgagcagatg ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg
    58261 agcagacaga taaattaggg ctcttagctg ataacgttga aactattgaa cagttaagct
    58321 atgagcaatg tgccttgatg gagcgagcaa tagcagctaa gagaaaagaa ttagataaga
    58381 agtgatatga gtgtttaaac cattaatcga ttcatattca gcggttctga aaaagttcaa
    58441 aggaaaagac ataagcgcaa ccatcaatga ggaagtgaac attgatcgac taaagacgat
    58501 gtatgacggc tacgatggtg atcgagtcat tgaaattcgt tttattgatc ctagacgttt
    58561 caccgtacag caacgaaact tcatctatgc gctgataggc gatattttta tcgatacagg
    58621 catgccaacg gacttctgga aggaattctt ctactttcgt tttgaaggtg tcacagggcg
    58681 caaaataagc ctgaaagacg aatcgaatac aactgtgagt gatgccaatg tcttagcaaa
    58741 tatcatctta gatttcatct ttgaacatca tattcctttc aaagaaggct atgagatttt
    58801 acctgcgaat caagaatatt acttctacaa atgcatcaca aaaagagtct gctgcatctg
    58861 tggcaaaaca ggagctgaca tcgatcactt tgacaaagcg ctaggaagac gaaagcgcaa
    58921 agaagttgat catgcagagt acacatttgc agcactctgc agaatccatc acacagagaa
    58981 gcacaaaata ggtgtgatta atttcaaaaa taaatatcaa atcaaaggga tcaaattaaa
    59041 ccagaaaaca atcaaaaagt taaatattgg agggtaaaaa tgacagaaca tcgaagttat
    59101 tatgcgatta taccagccaa cgtaaggtac gacaaaagac ttaaaccaaa tactaagttg
    59161 ttatacggag agataacggc cttgtgtaat gaaagaggct tttgttgggc aggcaatgag
    59221 tactttgcag atttatatgg tgtgaataaa gagaccatat cgcgatgggt aagtgatttg
    59281 attaagtttg gatacttgaa tcgggaaatc atttacaaag agggtaccaa tcaaataatc
    59341 aataggtacc tacgaattaa tcaataccct attgacgaaa aacgcaatac ccctattgac
    59401 gaaaaagtca aagataataa tacatctatt aataatacat ttaataatac aaaagaatat
    59461 ataagagagt taccgccttc gaaaaaatcg aaggctaagc ccgtccgtca taaatacgga
    59521 gagtataaaa atgttctttt gtcagatgag cagatggaga aactcaaaac agaattccct
    59581 aatgattacc aagagcgaat cgaacgactg tcagagtatt gtgaatcatc tggtaagact
    59641 tataaaaact atttggcaac tattcgaagt tgggcaagga aagaaaaaag tgaacctaag
    59701 aacgcaagca gtggatacaa gcgcacagga agacgagaga agcttcctga atgggcaatc
    59761 gaccaagaag cctatctcaa gaaaaaagcg ctagaacgag ctaatagaca atcaaaagca
    59821 ccattctaag aggtggaaaa atgaagatcg attatctaga actaattaat gaaatagcga
    59881 attataaaaa gggcgaggaa ttagacgtcc tgagagacgt atatgatcaa ctcgaagaag
    59941 ctggaattga aggaattaag aatgatcgtt cgagttggag taaactcaga tactatttcg
    60001 cactctatat cgatggaacg caattaagaa atttagcata taccaaatta ctattcattg
    60061 attgtgttaa aggcttgcaa aaacatctta atgaacttga gcaggtgtaa acaagatgga
    60121 cctaaagaca tttacagcac agatcgaatt aatgcatcaa gaagctttaa gacaaagtgt
    60181 gtcgtacgaa gacaagtggc tcaacacatt tcatggcgga cgtgagagcg cacttgatca
    60241 agtactcaaa ttactgaaag gagaatgtcg ggatggataa gaaagcggca atgcagcgaa
    60301 ttatcgaatt gacttattca gaagattggc aggaagataa aaaaatagtt gcagaagtcc
    60361 aaaagctcgg taaatcaatg tggactgaaa agcccaaacg gagaacgccg agaaagattg
    60421 caatctggca tggtgatcga attctagtaa cgggtaccgc taaacagtta tctgaaatta
    60481 ctggtctgag caaaaatatt atttgggata gagctaggag cttatggatt gattcaaaag
    60541 gaagacagtt taggtatgtg gaggagaaat aatggacgaa ctaatcgcga aagtagagca
    60601 gtgggcaaaa gataagggat tggatcaagc ggatttcagc aagcaaatgt taaaaacggt
    60661 cgaagaaatc ggggaagttg ccgcttctct agctagaaaa gatgaacatg gtttaagaga
    60721 cggaattggc gatgtagtag taaccttgat catcttggca atgcaaaatg atatggattt
    60781 atacgagtgc ttggattttg cttatgacga aatcaaaggt cggactggaa agatggtcaa
    60841 tggtgttttc gttaagtcca gtgacctgaa ataattccat taatggaaga aataactgaa
    60901 taggaggata gacatgattg atgcaaaaca agtgcaaaaa cagaaagatg gaatgttgat
    60961 gtttgaagca tatgttctac catttcttaa tcaatttgaa gttttggaat gctctgccag
    61021 cggagaagaa ttggagtatg ttgtaattcg tgaaaccaaa gaaaacgttc agaagctaaa
    61081 cgaatttctt tgcactatta actgttggga tatgatagct ccaggatttt tatgtccagc
    61141 tatgggagaa tttttggaat attgtcgtct tgaagatgca ggcactttgg atttagctta
    61201 tctggtatac aactacctaa acatcaacac agatcatttg tggtttggca ctgccgaaag
    61261 aaaatgggta gtccgctaac caccaatatc aatgaaaaaa ggcggtttcc tgaaagtgga
    61321 attgccgcca ttgctgtaag gttctttacg cgactcaagg tcacagaaat ataaatgcaa
    61381 atctcttttg aagtgatcca aagctctcgg aaaatggaag ttttttgttt caaagaataa
    61441 atgagatttg cgtcaagaac ctgagagtta tttccaaaaa agcttccaaa gaaccgaatg
    61501 agctaaggac atcatgtgta aacacataat atagctctcc tttcacggct aatcataaca
    61561 tgaaaaatat tcgatgtcag taggaaaaaa ggtgaattat gaaaagaata ttaaattatc
    61621 ccggcagtaa cagattctgc aattaaactc cgaagctgat gtatcagtat caactgtaga
    61681 atattatgag cgtggagaaa gtcaagcagt cacaaatgta aaaatcgata acgtcaacgg
    61741 tcttatctgt ttagatgaag gcgatagtat gaatgaaatt gctgcagacc atgaattatc
    61801 agaaactgct ttaattgatc aaatcgaatt agctagacaa tactttgttc catatgcaga
    61861 tacctggtcc aagcataaga atgacatcat ttttccagag gaacaatgag agcaactgat
    61921 ccagtaatta tccttgagga agccaaattt atttggactc acgaagagat agagcaagca
    61981 cgcttgctct tttctcaagg agttaagccg agcaaagtag ctgaaataat gggtcaaaag
    62041 attcttgatg tcggattgct tttgctccat ctagcagaaa aaaatttgat ttgaggtgaa
    62101 aatgatgatt caacttgcag gcatccaaac agggaaaatt tatttttctg gagaaagcaa
    62161 aagcgaagcc aaccaatggc tattgaaaac ttacacgaac aataagaagc taaggaaaaa
    62221 atatcctgat ttgttgctaa aggatgatca gattatgccg gaaccgatga ttttgacccg
    62281 taaggaggga agttgaaact aaaaaaccag ccgtgtgggc tggagttgct atttcttttt
    62341 aataatgagt ttttctaaat ttaattcatt ctcacgagtt tgcgtccaat aatcagaata
    62401 atattttaat tgtttaagag taggaatgtc gtcttcggta aaattagaaa ataattctgg
    62461 agaattatct ttttttaatt gttctaatcc tgattcttta aaacgttgtt caacgagttt
    62521 tactaaattg ttgatattca tgataaatca cctttttaaa tttaatttcg atttgattaa
    62581 tcgatacaag tattatatca aataaaaaag ctgatttagg gggtcttaca aaaacactaa
    62641 aattttttca aaaagaggag gaacggtggt gaaagtcaga gaacaattca agatagtgag
    62701 cgacgaagat aatgtttaca aaataaagat cgttaatatt aatgaatatc gagaagcaga
    62761 tcacaaatat ttggttgact tatacatgaa cgataaatat ttgggagaag gattttgtgg
    62821 agattcactt tttgatgatg agcgaatttt gaaggaggaa acagaatgat ccacgaacta
    62881 aaaatactac cagaatattt tgaagcagtg acgagcggac gcaaacaatt cgagatccgc
    62941 aagaatgatc gtgacttcaa agttggtgat cagttaatac tacgtgaata taaaaaatac
    63001 attaaacatg aattacctgg actaacgaga ccaacattta gttacactgg tgactcatac
    63061 aaagcagaga tcacttatat tactgattac atgcagaagt ccggatatgt agtgttgggg
    63121 attaaagggg tgaattaaat gaaagtatac atcttagcta tcacagaggg cacatggatg
    63181 ttcccagtcg gttccggaaa aatctataaa tcaaaaacag ctgcttataa agcatttgaa
    63241 aaatacaaaa aagaaaacgg tggcggcact aacgcaaaga ttctagtggc tgataactgg
    63301 catgaagaag gtgaacgtaa ttgatttacc tgttcatttt cgtggtacta actattttta
    63361 tgctagtaat cttattcaaa aaaagatgag taaagacgaa cgaaaaattc tggccaactt
    63421 ttatttagta ttaaaaatcg aacaaaatgt gttttcggaa aggagaattg tatgggaaaa
    63481 acaaaatcaa agattaagaa gaaaaaacga cgtttggaac aaaaggcaat ccagaatgga
    63541 acggctaaga aaaaataaaa aagctacccc tttcgagata gcagcgcaat actattctac
    63601 cataaggggt ggcgtttgtg agatttcatt ggttaaaaga ttaccaagaa ctagatgagc
    63661 agattcttta cttaaagtgg aatcttaata aaagtaagct tgaattgaat cgatgggtct
    63721 gtggtgattt agcaaacgtc cgcatcgaaa agaattcgag aacttcttct ttagaagaga
    63781 atatccaaaa gatagaaaat gaattggaat tgttgatcga gcaaaaagaa gaaatgttat
    63841 tgctgataga tagcttttct ggtatcgata atcaaattgt taagatgaaa tatgttgatc
    63901 aaatgagctt agaagatatt gctgaatctg ttgggtatag ctcatcgtat gtcagacagc
    63961 gacatgcaga aattagaaag acattgaact ttttagatga gtatgaacac agacaagctg
    64021 accgattgaa aaaagaaaac gaaattgatt tttacaatag cgagaagtac aaagaacaac
    64081 tctctttatt ctaaaattac actgttcact aaatgttcgg tcattgtagc tatgtaaaca
    64141 ttgttagcag catgatattc tattagtgtc aaaaaaatat gaaagagcca agatatccca
    64201 accgttttat taattggtat ctgtggctct tttattcttt taattagtag ttggcataaa
    64261 gttatttcca tttataaatt gatcagctaa atcagcatca taatcagccg cgataaattg
    64321 cgctgctgat ttaataaatg catccatatc atgaacatct ttattagtat gtctacgaac
    64381 atagtgcgtt tcatcattac caatccaagt agcagctttg gctaatcttt ggagtttagg
    64441 aaaatcattc aaataatttt caattgtttt acctaataac atttttttta ttttatcttc
    64501 atcctctgaa tttttagata ttgcataatc tttaattaga aattctagag cttttctata
    64561 accaactcca gcaatttgtt ccaattgctc agatttagct tgtgtggctt gagtataaat
    64621 atcaacaaat gtaggagaaa ctttttcaat attttctggt aaatctattt taatcggcgg
    64681 acggtaataa tctttaatta aaatattttc agaatatcct tcatcttcat taaacgtatt
    64741 tataaattgt aaactataaa atttgttgca ctcatcattt atacactgta ctaatacgcc
    64801 aaagattcta tcattatcga acgcactata tgatgaaacc ccaaaataca catacggagt
    64861 cattatattt ccgcaatgcg gacaactatt tggcaaataa attgtctctg tattttgacg
    64921 atcacgatac ttaatattaa cttcttgaag cattaaaaca cctcgcatct tttttataaa
    64981 tcataccaca aaatatctga taggagagta aacaaatgaa tgattttcat gaagcggtac
    65041 ttagtattga agtagaatca agtttggcta aagtttataa gaaagcaatt gaagcagaga
    65101 atagtccgta cagagaaaat tggaatggta atcatgcaca tgtgcaagtg gagaatgatg
    65161 attacagaac tggaatgaat acgttagtta tttctttatt gtcacacact ctacccaatc
    65221 tgcaggaaac gattgaatgg tatgagcgaa tgggcgcaaa agtaatccgt acaaattaca
    65281 aaggagagaa ttaaaatggc aatgatcaaa attagaacat ccattagtgg aacagagtat
    65341 tgggattcag aaaagaaaag gacagtagtt gttccgaaag atcaagaacc aaattttgaa
    65401 gtaaaagaag aaaaaggaat tttgagtgat ggcaaatcat ttgttgctgt caatggcgaa
    65461 ctcatagctc atagcagtga aattcttgat agcgatggaa acactgctgc taattttgat
    65521 ggagacgaag ctactaatga tcagtccact gaagatacgg atgaactgga caatatgact
    65581 gcaaaagaat tgcgtgcata tgctaagaaa catggtattg atattcctgg tgctatccgt
    65641 gcaaaaggcg atatcataaa tcttattcgt gaagtagaat gaaatactgt cagtttgacg
    65701 gatgcacgaa caaaatagca aagggtatct actgtactga acacaagaga tcaagcagat
    65761 cacgcaagaa gaagcagcaa gcaaagtctg tttatcatca tgagaacaaa ccattctatc
    65821 gaacgcaagc atggaaagat atgcgtcaat ttatttatga aagagaaggt ggtcattgtc
    65881 agcggtgtgg tcagttcata tttggaaaga gagcacacgt ccatcacatt gtaccaatca
    65941 aagacaacga actgcttaag cttgatccaa acaatctcat gctattatgt tcaaaatgtc
    66001 atccaattgt tgaaaacgaa acggaagaca aaaaagtttt tccttcgtat ttcaattgaa
    66061 gcccccctat tcattttcaa aattttttcg cgtggggaga tagggtagcg gggagtcacg
    66121 cgcatcgtta ggtcaaattt ttcaaaaaac aaaggggggt gtatacaaaa atgacgacta
    66181 aagcgcaacg taaagcgatt attgatgaaa aagtaagtgc tgaaaaagct cgtatcttag
    66241 aaataatgaa tttgtctgat ttgtacacca tcactcttga tccattaatc gaatcatact
    66301 tggatatttt tgaaatttac caacacaaat atttattgtg gaaggaaaaa ggatttccgg
    66361 agacgcaaaa attcacgaat aaatcaggag ctactaatca atcaaaacat ccattggcac
    66421 aacaagtaga aacttgggca gataagaaga tgaaagctct agatttatta ggattaacaa
    66481 acaaagcaaa aactggtaga ctaattgctg gaggatcgac cgcaagaaaa gatgaagaaa
    66541 ttacacgccc agaagtaaag ccagtagatg aactagcagc gcacagaaat aaatggcgta
    66601 agaaggcagg tgctgaaaaa tgattgaacc tggtgtaaat tatgctgatt tatttgcaaa
    66661 agaagtaaga aagaaaccta agaagtatcc taagaccgtt cgtttagcaa tagatcgctg
    66721 gtatcagtgg aaaaaaagaa aagatatttg gtttgatgta gatcgcgcga atgaaatgat
    66781 ggattgggta gaatcattta ttgttcacac aaaaggagaa atggtgggaa aaccgtttct
    66841 tttagaaccc tgggaaaaat ttatttattc ttggatttat ggttgggtta aagaaaatga
    66901 aaaaggccaa attgtacgcg ttactcgtga ggcttatgtc cagattccaa aaaagaatgg
    66961 taaaacattg attgctgtcg gttcattagg atatgcaatg tacggagaag gggcgttatc
    67021 agttgattgc tatgcttgcg catctgattt tgctcaagcg caatatgctg ccaaaccttt
    67081 tgcagttaca atcttaaaca atccaatcct acttgaagga actaaaatat ttagggggcc
    67141 aaagggaact gtttctagta ttacttatga ctatattcat gaagatatgg cttactcaaa
    67201 taaatttatt gttcaaacca agaatattga taatatcgag ggatccaatc catattttgt
    67261 attgaacgat gaattgcata aacaggagaa aatggaacag tatgacaatt ttaagtctgc
    67321 tcagatatct ttaccacagc cattgatgtt taatatctca actgctggga aaggttcgtc
    67381 gtctgttgga atgcgtgtat atcgcgaagc aaaagaagtg ttgaaacgtg atgataatga
    67441 ttcaaacttt gttctaatct atgagccaaa taaaaattat gattggacgg atagaaaagt
    67501 ctgggaaatg tgcaatccta actggggaat atcagtcgat ttatctgctt tagaatcggc
    67561 atttaaaaca gcgcaacggt cagctcattc caaagctgaa tttctaacga aacacttgga
    67621 tgtatttgtg aacggcgcgg ataatttctt tgaacaggat caagtagagc catgtttagt
    67681 taccacacaa gaacttggtg atttaagtgg cgaaccatgt tatatcggtt tagatttatc
    67741 acgtacacga gatttgacct gtgtatcttt aaacttccca acatgggatg aagatggtaa
    67801 agcggttctt aaagtaaagc aactttattt tattccgaat gaagatttag agtttcgaga
    67861 aaaagaagat aatgttcctt acagtgattt ggctgaacaa ggtttcgttg aattttgcga
    67921 cggaaaaatg attgatcaag atcagatttt gcagtacata gaggactgca tgaatttata
    67981 tgatattcag caagtgaatt atgatccagc gatgagtgac aaacttgttg agaaattaga
    68041 aaacttagga ttggaatgcg ttgaagttgc tcaatatcct aaagtattga atgctccttt
    68101 tgacgatgtc gaacggttgt tttatgagaa acgaattcaa tttgataatc cattattcct
    68161 ttattgcact ttgaacgttg tagcaattac taacatcaat ggacaaaaag cgccaagtaa
    68221 acgtcaatca aagaaaaaga ttgatgggtt cgtggcgttt ttgtgtggtc ataaggaaac
    68281 gatgaatcaa atgacagata ttgattcaga tgagttagat gattacctaa gttccatcta
    68341 tagataaata gaaaggcggt gaaaaaaatt gaaattacgt gatcggttat cgaatgcagt
    68401 ctattctttt atggaaaaac gtggatatat cgaggatatt tttggacact acacacgtta
    68461 tggtcaaaga tatgtgacgg attcatctat catggaatct tctgatgttt atgaattagt
    68521 tcaagacata tctaatcagg tggcattggc cacaccaatt gttattggtc ccgacggcaa
    68581 cgaagtcaaa gatcatcact tactaaatat tttgaagagt cccaatgatt atttgacagg
    68641 attcgaattt accaaattgg aaacaaatac tttattgatc aatggtgaga cattcccatt
    68701 aacggatagg gatcagcttc atttggcgta cggtgtaaca actaaaatca atgaacgact
    68761 tcaagaagaa tttgaaatga atggtcaaaa aatacctggt caaatgattc gacacattaa
    68821 gaacatcgga actgattcat taaaaggtgc tggaataatt gatcttgcaa gaaacactct
    68881 agaaggcgtt ctgagcgctg aaaaagtttt gacggacaaa tatactaaag gcggtttact
    68941 tgcgttcatg cttaaactag acgcccacat caatccaaat aatagcgccc aaacaaaaat
    69001 tgtcaaagct atattggatc aactggaagg aacgcaaaat gagagtgatc attctgttaa
    69061 gatgattcct ttgggaaaag gatattccat cgagacatta aaaagtcctg ttgatgacgc
    69121 ggcaattttg aattatttag gtgtttacaa aaaagactta ggaaaatttc taggaatcaa
    69181 tgttgatacg tatcaatcgc tgatgaagac agatattgaa aaagcgatga tgtatctgca
    69241 caacaaagca atcaaaccaa tattgaaaaa caagagcgaa cattacaccg ctcttttttt
    69301 tatgcctaat tctggctata gagtggaatg gaaaattaat attttggatt ttgttcctta
    69361 ctcaacaaaa acaaatattg gctacaacat cgttcgtacc gggattacaa gtccagataa
    69421 cgtggcagaa atgcttggtt ttcctaaaca gaatactcca gaaacacaag ctatctatat
    69481 ttcaaatgac ttatctagga ttggccagaa aaatgcaaca gatgattcct taccaacgaa
    69541 tgatcaagac ttgaaaggag gtgatggaaa tgaagaagaa ggaaattcgc acgattgaca
    69601 tcaccaatct ttcaacacgt tccgatgaag aaactcatac gaggaccatt agcggctacg
    69661 ctgctgtatt caacagtcaa acacttttat gggatgactt aagcgaagtg attgttcctg
    69721 gtgcattctc aaaggcaatt agtaattcgg atgttcgttg tttgttcaat cacgattggt
    69781 ccaatgtgct aggacgtact aaaagcggaa cccttcgcct tgaagaagat gaacgtggtt
    69841 tgaaattcga agttgattta ccggacacaa cggtagcaag ggacttagtt aaatctatgg
    69901 aacgaggaga catcaaccaa tgtagttttg gttttgtgcc gactgaagaa acgtgggact
    69961 acaattctga accaatgctt cgaacaatcc atgaagtgga attatacgag gtttctattg
    70021 tgcctttgcc agcatacgaa gatacagaag ctgcattaag aagtcgtgat gaattagaaa
    70081 aaaacgtcga agaaagaaaa aaattaatca aaaaaattaa tcaagcgcta gaagcgtagg
    70141 aggaaaaata catggataaa gaattattga aaaaaatgaa ggcacgtcgt gagcaacgat
    70201 tgactgaatt acgtgaaaaa gttgaatcag gagaattacg cgaagcagat ttagaagccg
    70261 tgaaagaaga aattgacagt gttattgatg aattgaacgg aattaaagac gaattaggcg
    70321 cagattctgg aactgatgaa acagacgaca atacagatga tcaatcgaat agtacggatt
    70381 cagacgaaag ccgttctggt gaagacaacg atcaagaaga agattcagac agtgaagata
    70441 gttcagaaaa tcgttctgga atgattactc aacagcaacg agatggatta cttggatcaa
    70501 ttaagaacgg attggaggca cgtgcaaaaa tgaccaagaa acaaaaagat caacaactac
    70561 gaaaagcatt tgctaatttt gtagctggaa atatttctga agcagaagct cgagctttag
    70621 ggattgaagc tggcaacggt tcagttactg tcccagaagt aattgcatct gaagttatta
    70681 cttatgctca agaagaaaat ttacttcgta aatacggaac agtggtgcga acatcaggag
    70741 atgtcaaata tccaattctt gtgaagaaag cagatgcgaa tgtaaacaag aaagagcgtt
    70801 caactgatat tgctgaaaca gctattcagt ttgatgaaat tttgcttaat cctgccgaat
    70861 tcgatgcttt ggcaacagta actaaaaaat tattaaaaat gtctggtgtt ccagttgaag
    70921 atattgttgt ggaagaattg aaaaaagctt atgtgcgtaa agaaatcaat tatatgttca
    70981 atggtgatga cgctggaaat gaaaatcctg gtgcattagc caaaaaggct gtagcattta
    71041 aaaaaccttt agatctaact gcttcaggtg ctgggcaaaa attatatgat gcattaatcg
    71101 aatttaaaaa tacaccagtg acagaagtga tgaaaaaggg acgctttatt attaatcgag
    71161 ctgctttgac tgccattgaa aaaatgaaaa cagatgatgg atttcctttg ttgcggccat
    71221 tcacacaagc agaaggtgga attggttacc aattagttgg ctatcctgtg gattggacag
    71281 atgcagcaga taaaaagggt gaaccagaca cgccagtttt atattttggc gatttttctg
    71341 catttaaaat tcaagaagtt attggtgcct tggaaattca aaaacttgtt gaaaaattct
    71401 ctggtaaaaa tcaaattgga ttccaaattt acaacttgct agatggtcaa ttggtttatt
    71461 ctccatttga accggcagta tatcgctacg aaattacaaa accagttggt ggttaagatg
    71521 gaagagcaaa ctaaagaatt gtctttagag gaaaaattca aatcacatat tcattttgaa
    71581 gagggcatgg atgattcttt gctctctttt tatttaaata tggcaaaaga ttatgtcaaa
    71641 acagcaactg gtggccaaca agaatatctt attttgatgg ttgccggcat tgcctatgaa
    71701 tatagagttt cagaggatga actcgacaaa gctatgaatg ccatgacgcc atttatcgtg
    71761 caaggagcga ttcaaaatgc cgaagagaca gaccaataat ctcagatgga aagccgaatt
    71821 gctagacatc aaaacaggaa cagacggaaa cgatcgtcca actacaattt acgaatttaa
    71881 gcgtctaata ttctatgaag aactcggtgt gacttctcaa gaaaaatatt tatcacagca
    71941 agccaagaca gacgttgtca gacgaattaa agtcagatgg gataaatcca tcacagagaa
    72001 attaagtgcg ctcaaaattg attctgtaac gtataacatt actcgcattt atacgaatcc
    72061 cgatgcaaga gaaatggagt tgagtttagc ttatgtcgat tagctttgat gaattgaaaa
    72121 cagcgctgaa atcaacaaag ttagcagtgt tcagagacaa agccagatta gggacgatgt
    72181 atccatacat tgtgtactca aatgtgagta acagcaaaaa aatggcatcc ggtaaagtct
    72241 ataaaaaatt accttattac caagtctctt tttttacact tggaacagaa caggatttag
    72301 ttgttattga acaagcgctg caaaatgctg gcattccata ttcagatttt acaggtatac
    72361 aaggtgatga gaacgacgat accgtaacca actattacac atatgtgagg tgtgtggaaa
    72421 atgccaagta ataagaatgg tttttctgaa atatcggatt atttaggaaa cctttcaaaa
    72481 gttgatccga aaaaattatc gttggaatct ttagaagaag cagctaactt ttatctaaag
    72541 cagttgcttc ctaatattcc taagtcgctc ttaaaaaaga aacatatgcg cgatcaaata
    72601 aaagtagtag ttgaaaacga tcaagtgaaa gttcaatttg aagagacggc cttttactgg
    72661 cgctttgccg aaaatggcac aacaaatcaa cgtgcccaac attttgcaag tggtacgtat
    72721 gaacaaaaca aagaaaaaat tgaagaaatt atgactagaa aaatacttga tttatgggaa
    72781 gggtgatttt aattgggaaa acaagatact ttttattttg aaggattgga cgacatttta
    72841 attgcgatga tggcaacgcc agattctgtt ggaactgcac cgacttatag tgaagtttta
    72901 cgattgccaa ttgccacaaa aatcggtgtc aaagggaatg gaacagcatt agaaaaatgg
    72961 gcatccagca aaatgtttcg tcgtgtgtcg cgagaaacaa aacatgaact tgcgttggat
    73021 cacgtgggta ttccgataga agtgatggat gaaatcaaag gattagttgc taaaagcgga
    73081 gtaacgttta gcaaaaatac cgcacgagaa tttccgtact tcgcctttgg atttattgga
    73141 aatattgaaa atggcggtaa aaaagcagtt tggtatccta atacacaact atccaatgtg
    73201 attgacgaag agtatgccac tgcagaggaa gaaacaaaaa ttgacgatgt aactgcaaat
    73261 ctggtttcca tcggactgaa atacaataat gtgatgtatg caagttttga ttcaaataga
    73321 gacggagctt ctctagaatt atttaataaa ttcattgctc aacctgtata cgatgaagaa
    73381 cagtggaaaa cattggctaa agttggaggt gcgggttaat ggctcggtta tctgattatg
    73441 gaattcacgt tgaagactta aaaaattctg ctgttgtaat tattcaaggc gcagaattcc
    73501 ctatttcatt tactatgcaa acgatggaat ttatagcaga tgtgtatggt ggagattatt
    73561 cgcaatttga atctgatatg aacgccatgc tatctaaaaa agaaggacaa atttcttctg
    73621 ctaacttatc gcctagtgat ctaaaaatca tgcgtgcctt gatttatgcc atgctgcgta
    73681 ctggtggttt agaggaagat ccagaaacta ttttcaaatt tttgggaatg agtggagagg
    73741 tgttgtctgc ttatagtacc tgtatggaaa tttttgctag ccagacattt caggtggaag
    73801 acctaaaaaa atccaagaag ccacaagact ttcaaaaagc gcaagcaaaa agaaagaaaa
    73861 acaaaaagaa tcggaagaga taggaactcc ttggagtttt tatatttacg ttgctctcac
    73921 tctattgaat tggagtgaga gtttcttttt aaagtctaca cctaacttgt ggctcaaatc
    73981 ttatttacag tggttacaac aaaacaccga ttttgaacca cctcaatctg taactatgga
    74041 taaatcgcct tggtggtaga aaggagcgct aagataaatg gctggtaaag aatctgatgt
    74101 cgttcttaat tttaaaacga atggcgaagt cagttattcg aaaacaatca aagaaatcaa
    74161 caaagaaatg aacttagccg ctgccgagta caaaaaccaa gtgtctgcga tggacaagga
    74221 tgcaactcaa acagaaaaat tgcgagcggc taaacaaaaa ttagaaaaac aattaggatt
    74281 ggctgaacaa cgatctcaaa tgctgagaga ggaatatgaa aaatctgtca aagaaaccgg
    74341 tgaatattct gctgaatcag aaaaacttta taaacaatta ctcaattctg aaaccggaga
    74401 aaataaactt cgtacagcat tagaacagac gaacgatgca cttaaagaac aaggtgacgt
    74461 ttctgttgat acagcaaaaa aactccagaa aatcgaagaa acaggtgaga aagtaaaagg
    74521 cgttggtgaa aaaatgtctg ttggagtaac cgcgcctatt gtagcggcag gagcagcagg
    74581 acttgcagca tttggtgaag ttgacgaggc acttgatacc atcattacaa aaaccggagc
    74641 aacaggtgat caagctgata gactttcaca gtctttcaaa aacgttggtt caaatactca
    74701 tttaccttta caaacggttg gggaagctat tggtgaggta aatacacaat ttggattcat
    74761 ggataaaaaa ctggaagatt caaccaatta tctcctacag tacgctgaaa tcaatgatac
    74821 agatgtttcg caatcagcaa tatctgctcg acaagctatt gatgcttatg gactagaata
    74881 tgatgatttg aattctgtcc ttgatgtaac aacgaaaaca tcgcagaata ctggtcaatc
    74941 tgtagacgac ttgatgcaaa aagcaattga tggcgcacca caaattaaac aattagggtt
    75001 gagctttggt gaaggggtca ctttacttgg acaatttgag caatctggtg ttgattcaag
    75061 cgcagcttta agtagtttat ctaaagcaac agtggcttat gcgaaagacg gaaaatcttt
    75121 aagtcaagga ctagaagaac tacaagacaa ggtaaaaaat gcaggttctg aaacagaagc
    75181 catcaatgca gcagcagaag tattcgggac taaaggcggt cctagaatgg ccgatgcaat
    75241 tcgtagaggt actttgaacc tggaagattt agcaaaaact gctggagaaa gtgggggagc
    75301 tgtaggagac acattcgatg ctactctcga cccaattgat caagccgatc aagctatgaa
    75361 caatgcaaaa ttagcaatgg ctgacgtagg tgaagcagtc caagtaagcc ttttgccttt
    75421 ttttgaaaaa gcaacttcct tattacagga attctctaaa tggtgggggt ctctagatca
    75481 agatacaaag aattggatca taacaattgc tggtatagcg gcagcagttg gaccagtact
    75541 aatcgttctt ggttcactta tgggatctgt tacaaaaatc gttggggaag ttaaaagttt
    75601 tataggagtt tggcaaggac ttgctggttt atttggaatg tcgggaggct ggtttgcatt
    75661 agcagttata gctattggcg cattagttac tggactaatt tgggcatata ataacgttaa
    75721 atggtttcat gatggagtga attctttctt caaaggtgtt tcagatgtag ctgttgaaat
    75781 atttaatttt ttaagtggtt attttggtgg atttttcgat ggtgttatag caaatttcaa
    75841 taatttttat aatgctggaa aacgaatttt cagtggtttt ctagatttta tcactggagt
    75901 atttacaggg aattggtcaa aagcatggca aggtctagtt gatatttttg gaggtatttt
    75961 cgatggtatt gttgctgtag ggaaagctcc tattaatgcc atgattggat tgatcaatgg
    76021 atttattggt ggattgaata atataaagat accaaaatgg gttcctggtg taggtggaaa
    76081 atcattttct atttcaaaac tgccttattt agcacaaggc ggtcatctaa tcaatggaca
    76141 agcgattgtt ggtgaagctg gacctgagtt actgactgcc aagaatggta aaaccacagt
    76201 tactccatta tcagacgaag aaaaacgcaa agggatcagt ggaagagttt ctggaggtag
    76261 cattgaacag catatacaca ttggcaaagt cgatgcaaac aatccatctg agttagatcg
    76321 tatgaatcgt aaatttgcta aagcaaacaa acaggctatt cgtgatttgg gaggtgttcc
    76381 tatttgagtc gacaatttat gaatccagat gaaccaaatt ttatttggaa aaatcggaat
    76441 gcagtaattg atatgaattg catcattgag gcagagcttc cagatattat gccgaacaag
    76501 cgatacgaaa cttacacgat tcagggccga agcggagaat tgacggagac gtttgatgac
    76561 tatgaacctt ttgatttgga aatagaaggg atcactattc cacattcgaa actgagagaa
    76621 gtcaaacgat ggctcactgg taaaagccaa ttaatcacac ataatgatcc agacaagtat
    76681 ctagaggcta tctgcagcat ggataaagaa gtccctttcg aaaacgagtg gggcttcttt
    76741 tatacattcg atgttacttt tcgttgtcag ccactcaaaa gaaaaatcgg tgaagcacct
    76801 aaaatttttc attctagtgt tatggatatt tttgatcatg gtgacgaaat tgcacatccc
    76861 tatctggaaa tagaatcgaa cggaggagat ataacgatcg ctgtcggtga taaaagccta
    76921 acaatactta atacgttagc tgcaacaatc gtggttgata ctcagcttgg aaaagcaata
    76981 caagaaggtt tgaatttatt tacgcgtggt gattggccag tattgcagcc tgaatggaat
    77041 caggtaaaaa tatccggaaa atttaaagaa gtgcggtttt ggaataggag tgtgtatctg
    77101 tgacgcaaga atttatttat gcttataaaa aaatgccaga tgatttgagc gtgaacggag
    77161 cgtcgttagt tgattgggaa gatttgccag aaatcaatcg tgtgctgaat ggccaatatc
    77221 gtttttatgg taactattca agaagcggac agtatcgctc ttatttaaaa aaaggaaatt
    77281 ttataaaagc gaaggtacca gatggatcat ggcaatattt tgaaatttat aatgtcaaaa
    77341 agaatttgac ctccgtttct gtaacagctc ggcatattgg ttttatggcc aataagaact
    77401 ttattgtcaa gtcgtttacg gataacggca atgggtctca gatcatgacc aatcttaaaa
    77461 acagtttggc atttgatcaa aaatttaact atctttcgaa tgtagggaca acgcaccaat
    77521 ttacagcaag acaagtcgcg ccagttgaag caatcattgg ttcaaataac ggtaatcaaa
    77581 atttgactgg cgtgactagc gcggaacttg atatggataa ctatgatttg aaactggtaa
    77641 aacagattgg atcagataat ggttttcgga ttgattttgg catcaattta gaagcaatcg
    77701 aagaagaaat tgatgaagaa tcaattgtca atagtttgta tttggttggc ggtgttcctg
    77761 ataacgatta tgatgaagac aaagaaccta tcgaatatgg ttatttagaa attgatggag
    77821 taaccaacga gaatcgacgt attgccaaac gtgaaaacgg agattgtaaa acagttgatg
    77881 aattgatcaa atggggcaaa accttatttg acaatgatcg tatccatgag ccaaaagcta
    77941 ctcatacagt cagtatggtg gcgttggaac acacactcga atacggtgac atgtatcaag
    78001 agctagcgtc tttacacttt ggtgatgtcg ctcatgttag ggcgaaagag ctagatatcg
    78061 agataaaaga gcgtatggtc gaatacactt atttcccaac gcttgggaaa tataaggata
    78121 ttgttttagg taatgattta tcactgtaca cttcggcagt aaatactcaa gcacaagaat
    78181 tgaaaaagaa aatcgacaat cgaacagaaa cgttagttca aaatgtgttg aatgcgacgg
    78241 catggatcac tggtaattct ggtgggcatg tcgtttttcg tccagaaaaa gctccatcag
    78301 agattcttat catggatact ccaaatgtgg cgaatgcaaa gcgagtgtgg cgttggaatt
    78361 taaatggctt aggttattca gacaacggag taaatggccc gttcggtatt gctatcacat
    78421 caaaaggaga aattgttgct gacttcatca aagtaggaac aataaatgca gaagtattcg
    78481 aaacttcctt taatgcttat ggtgatgtgt taaaacttgt aaaaggcacg cttcaaattt
    78541 ggaatgaaaa caaaaaaatc atggagctaa ccaaaaaagg tatggaattc tggaatagaa
    78601 aagaatccat tggaacgatc ggaacaacag attcagcagg aaatccattt ccaaatgctg
    78661 taacacctac acctcttgag gaaaattctt tggtaatacg tacaaatggc gatggaaaat
    78721 acattttaat ttctccaact gcagaaaaag gatttgtgtt attaggaaac ggaaaagcat
    78781 attatttcgg tgatttagat attcagggaa aacttactgt tagaggtaaa gaagtgatac
    78841 caggacaaaa tggcggtcca tctggcggag gagaaacacc tggtgggtat cctgatgaat
    78901 taaaaacaga tgcagaaaag agagcttgga gaatatacga tattttgtgt aataacggat
    78961 ttacgaaaca atctgcatgt ggaatattgg ggaacattca acaagaaaca ggaggaactt
    79021 ttgatcctga tactgttcaa ataggtggac cagcatacgg attagttcag tgggatggtt
    79081 cttcatatcc tcttgttggt ccagcaacat gggatggaaa agtttatgtt caaaacttat
    79141 ttaatgctgc aggtattaaa gaaccaataa cgagcttaga tgcacaagtt cgattgctta
    79201 tttggacatt tacaaatgga caatggatgg gcgtagtaca acctacgacg gttgatggat
    79261 ttaaggcctg tactgatcca agacaagcag catatgcttt tgaacgaaac tatgaacgtc
    79321 cggcagcgac acatcctgaa cgtcaagatt atgcagttaa ctggtataac aaatttaaag
    79381 atttaaaacc aggaggagct actggagaag caggactaaa acatttagag tctttgattg
    79441 gacaacgaat tggcaatggt cagtgttatg gcttgtctgc agaatattca ggatatctgg
    79501 gtggttgtgg catgggtgct ggaacaaaat atggtttaac tcatgtgata ggaaatactt
    79561 ctgcagcatc tgatattggt attgcctatg attggtctgc tgttggttgg aaagtgattc
    79621 aaaaccctag atatgatcaa ttagtagttg gtgcaattat taattgggca agaggtggac
    79681 aagtgggttc atggtttgca gacggaactt atggacatac tggcgttatc cgaggcttag
    79741 ctaatggtcg tatgcaaact tatgaacaaa acacagagtt aggtatgatt tgtggaaagt
    79801 tagatcgcca gtattatagt gcaagtgcaa tttcttccat tgtcatacca ccaaaatagg
    79861 gaggtgattt aatggcaaag tggaatgtca tattaagtac aacagaaccc tataattacg
    79921 tggggatgat tcaagttcga caaggcaata aaaacactga agttatggaa gctacaattg
    79981 tcgaaaatgg tcttccatat gatttatcag aatgtaaggt atattttgaa tcagttgttg
    80041 gcggaaaata tccagtccaa ttagaaacaa gaattgtgga tgctaaaaaa gggaaaatca
    80101 a
//
LOCUS       DACCCM010000005        73467 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCCM010000005 DACCCM010000000
VERSION     DACCCM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.005"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            5..1087
                     /locus_tag="IVK78_000336"
     CDS             5..1087
                     /locus_tag="IVK78_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6373835.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1308..2450)
                     /locus_tag="IVK78_000337"
     CDS             complement(1308..2450)
                     /locus_tag="IVK78_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6373836.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2455..2640)
                     /locus_tag="IVK78_000338"
     CDS             complement(2455..2640)
                     /locus_tag="IVK78_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6373837.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2994..3239)
                     /locus_tag="IVK78_000339"
     CDS             complement(2994..3239)
                     /locus_tag="IVK78_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6373838.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3233..3634)
                     /locus_tag="IVK78_000340"
     CDS             complement(3233..3634)
                     /locus_tag="IVK78_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6373839.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IVK78_000341"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IVK78_000341"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP6373840.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="IVK78_000342"
     CDS             complement(5649..7415)
                     /locus_tag="IVK78_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP6373841.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="IVK78_000343"
     CDS             complement(7428..8222)
                     /locus_tag="IVK78_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6373842.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="IVK78_000344"
     CDS             complement(8232..9608)
                     /locus_tag="IVK78_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6373843.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVGLILFFLYPLSKDKVLENC
                     ELLKQKYK"
     gene            complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IVK78_000345"
     CDS             complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IVK78_000345"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6373844.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10468..11895)
                     /gene="uxaC"
                     /locus_tag="IVK78_000346"
     CDS             complement(10468..11895)
                     /gene="uxaC"
                     /locus_tag="IVK78_000346"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6373845.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11969..13048)
                     /gene="uxuA"
                     /locus_tag="IVK78_000347"
     CDS             complement(11969..13048)
                     /gene="uxuA"
                     /locus_tag="IVK78_000347"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6373846.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13051..14682)
                     /locus_tag="IVK78_000348"
     CDS             complement(13051..14682)
                     /locus_tag="IVK78_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6373847.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14988..15659
                     /locus_tag="IVK78_000349"
     CDS             14988..15659
                     /locus_tag="IVK78_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6373848.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQHGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            complement(16362..16717)
                     /locus_tag="IVK78_000350"
                     /pseudo
     CDS             complement(16362..16717)
                     /locus_tag="IVK78_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16901..17308)
                     /locus_tag="IVK78_000351"
     CDS             complement(16901..17308)
                     /locus_tag="IVK78_000351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373849.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            complement(17809..18003)
                     /locus_tag="IVK78_000352"
     CDS             complement(17809..18003)
                     /locus_tag="IVK78_000352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373850.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            complement(18017..18355)
                     /locus_tag="IVK78_000353"
     CDS             complement(18017..18355)
                     /locus_tag="IVK78_000353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373851.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            complement(18399..18731)
                     /locus_tag="IVK78_000354"
     CDS             complement(18399..18731)
                     /locus_tag="IVK78_000354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373852.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18745..18975)
                     /locus_tag="IVK78_000355"
     CDS             complement(18745..18975)
                     /locus_tag="IVK78_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373853.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(19197..19733)
                     /locus_tag="IVK78_000356"
     CDS             complement(19197..19733)
                     /locus_tag="IVK78_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373854.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(19754..20845)
                     /locus_tag="IVK78_000357"
     CDS             complement(19754..20845)
                     /locus_tag="IVK78_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6373855.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20853..23063)
                     /locus_tag="IVK78_000358"
     CDS             complement(20853..23063)
                     /locus_tag="IVK78_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373856.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(23079..25607)
                     /locus_tag="IVK78_000359"
     CDS             complement(23079..25607)
                     /locus_tag="IVK78_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6373857.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEYKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(25661..26071)
                     /locus_tag="IVK78_000360"
     CDS             complement(25661..26071)
                     /locus_tag="IVK78_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373858.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(26084..26302)
                     /locus_tag="IVK78_000361"
     CDS             complement(26084..26302)
                     /locus_tag="IVK78_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373859.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(26299..27291)
                     /locus_tag="IVK78_000362"
     CDS             complement(26299..27291)
                     /locus_tag="IVK78_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6373860.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(27370..28281)
                     /locus_tag="IVK78_000363"
     CDS             complement(27370..28281)
                     /locus_tag="IVK78_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6373861.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(28300..28644)
                     /locus_tag="IVK78_000364"
     CDS             complement(28300..28644)
                     /locus_tag="IVK78_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373862.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28664..29221)
                     /locus_tag="IVK78_000365"
     CDS             complement(28664..29221)
                     /locus_tag="IVK78_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373863.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(29301..29801)
                     /locus_tag="IVK78_000366"
     CDS             complement(29301..29801)
                     /locus_tag="IVK78_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373864.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(29802..30347)
                     /locus_tag="IVK78_000367"
     CDS             complement(29802..30347)
                     /locus_tag="IVK78_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373865.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(30344..31600)
                     /locus_tag="IVK78_000368"
     CDS             complement(30344..31600)
                     /locus_tag="IVK78_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6373866.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(31963..32277)
                     /locus_tag="IVK78_000369"
     CDS             complement(31963..32277)
                     /locus_tag="IVK78_000369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373867.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(32404..33597)
                     /locus_tag="IVK78_000370"
                     /pseudo
     CDS             complement(32404..33597)
                     /locus_tag="IVK78_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(33787..34143)
                     /locus_tag="IVK78_000371"
     CDS             complement(33787..34143)
                     /locus_tag="IVK78_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373868.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(34209..34661)
                     /locus_tag="IVK78_000372"
     CDS             complement(34209..34661)
                     /locus_tag="IVK78_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373869.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(34663..35028)
                     /locus_tag="IVK78_000373"
     CDS             complement(34663..35028)
                     /locus_tag="IVK78_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373870.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(35045..35239)
                     /locus_tag="IVK78_000374"
     CDS             complement(35045..35239)
                     /locus_tag="IVK78_000374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373871.1"
                     /translation="MEKKNINICFLSGKECTYEECTDISVHERDGMLIVDMSPSDEPF
                     VVKHYFPLCNIESFRIDCEF"
     gene            complement(35256..35501)
                     /locus_tag="IVK78_000375"
     CDS             complement(35256..35501)
                     /locus_tag="IVK78_000375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373872.1"
                     /translation="MEEIFFCMMVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSI
                     IIFFILACVWILAREIYHQFVLEDGKGIITWFLQKRK"
     gene            complement(36217..40512)
                     /locus_tag="IVK78_000376"
     CDS             complement(36217..40512)
                     /locus_tag="IVK78_000376"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP6373873.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            complement(40659..40898)
                     /locus_tag="IVK78_000377"
     CDS             complement(40659..40898)
                     /locus_tag="IVK78_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373874.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(40988..41260)
                     /locus_tag="IVK78_000378"
     CDS             complement(40988..41260)
                     /locus_tag="IVK78_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373875.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(41343..41594)
                     /locus_tag="IVK78_000379"
     CDS             complement(41343..41594)
                     /locus_tag="IVK78_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373876.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(42592..43503)
                     /locus_tag="IVK78_000380"
     CDS             complement(42592..43503)
                     /locus_tag="IVK78_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6373877.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(43700..45535)
                     /gene="lepA"
                     /locus_tag="IVK78_000381"
     CDS             complement(43700..45535)
                     /gene="lepA"
                     /locus_tag="IVK78_000381"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP6373878.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            45682..46902
                     /locus_tag="IVK78_000382"
     CDS             45682..46902
                     /locus_tag="IVK78_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6373879.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            46922..47416
                     /locus_tag="IVK78_000383"
     CDS             46922..47416
                     /locus_tag="IVK78_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6373880.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            47409..48080
                     /locus_tag="IVK78_000384"
     CDS             47409..48080
                     /locus_tag="IVK78_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373881.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(48231..50840)
                     /gene="clpB"
                     /locus_tag="IVK78_000385"
     CDS             complement(48231..50840)
                     /gene="clpB"
                     /locus_tag="IVK78_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP6373882.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(51091..52137)
                     /locus_tag="IVK78_000386"
     CDS             complement(51091..52137)
                     /locus_tag="IVK78_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP6373883.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(52148..52420)
                     /locus_tag="IVK78_000387"
     CDS             complement(52148..52420)
                     /locus_tag="IVK78_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP6373884.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(52455..53363)
                     /locus_tag="IVK78_000388"
     CDS             complement(52455..53363)
                     /locus_tag="IVK78_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP6373885.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(53495..54727)
                     /gene="pepT"
                     /locus_tag="IVK78_000389"
     CDS             complement(53495..54727)
                     /gene="pepT"
                     /locus_tag="IVK78_000389"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP6373886.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(54742..55863)
                     /locus_tag="IVK78_000390"
     CDS             complement(54742..55863)
                     /locus_tag="IVK78_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP6373887.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(55860..56561)
                     /locus_tag="IVK78_000391"
     CDS             complement(55860..56561)
                     /locus_tag="IVK78_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP6373888.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(56705..57145)
                     /locus_tag="IVK78_000392"
     CDS             complement(56705..57145)
                     /locus_tag="IVK78_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6373889.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(57274..58200)
                     /gene="dnaI"
                     /locus_tag="IVK78_000393"
     CDS             complement(57274..58200)
                     /gene="dnaI"
                     /locus_tag="IVK78_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP6373890.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(58200..59573)
                     /locus_tag="IVK78_000394"
     CDS             complement(58200..59573)
                     /locus_tag="IVK78_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP6373891.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(59596..60087)
                     /gene="nrdR"
                     /locus_tag="IVK78_000395"
     CDS             complement(59596..60087)
                     /gene="nrdR"
                     /locus_tag="IVK78_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP6373892.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(60399..60731)
                     /locus_tag="IVK78_000396"
     CDS             complement(60399..60731)
                     /locus_tag="IVK78_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6373893.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(60783..61412)
                     /locus_tag="IVK78_000397"
     CDS             complement(60783..61412)
                     /locus_tag="IVK78_000397"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP6373894.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(61376..62212)
                     /gene="mutM"
                     /locus_tag="IVK78_000398"
     CDS             complement(61376..62212)
                     /gene="mutM"
                     /locus_tag="IVK78_000398"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP6373895.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(62257..64902)
                     /gene="polA"
                     /locus_tag="IVK78_000399"
     CDS             complement(62257..64902)
                     /gene="polA"
                     /locus_tag="IVK78_000399"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP6373896.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(65028..65711)
                     /locus_tag="IVK78_000400"
     CDS             complement(65028..65711)
                     /locus_tag="IVK78_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP6373897.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(65704..66189)
                     /locus_tag="IVK78_000401"
     CDS             complement(65704..66189)
                     /locus_tag="IVK78_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP6373898.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(66496..67830)
                     /locus_tag="IVK78_000402"
     CDS             complement(66496..67830)
                     /locus_tag="IVK78_000402"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP6373899.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(67961..68722)
                     /locus_tag="IVK78_000403"
     CDS             complement(67961..68722)
                     /locus_tag="IVK78_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6373900.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(68738..69220)
                     /locus_tag="IVK78_000404"
     CDS             complement(68738..69220)
                     /locus_tag="IVK78_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP6373901.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(69240..71420)
                     /locus_tag="IVK78_000405"
     CDS             complement(69240..71420)
                     /locus_tag="IVK78_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP6373902.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(71564..72295)
                     /locus_tag="IVK78_000406"
     CDS             complement(71564..72295)
                     /locus_tag="IVK78_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP6373903.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(72364..73305)
                     /locus_tag="IVK78_000407"
     CDS             complement(72364..73305)
                     /locus_tag="IVK78_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP6373904.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
BASE COUNT        21571 a        15399 c        11397 g        25100 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaaaa
     1261 aaaaaaatag gctgtgacag cagcgtgaca gcaaaaaaga tggaaagtta aatatcaaac
     1321 ctcgtaattt catttgcgag tgcttgattt gtttctggat ataagtgtcc atatttgtcc
     1381 aaagtagttt taatagaggc atgacctaga cgcttagata ttgcatagat atttttatta
     1441 tgttcaatta aaaaggcaac atgcgagtgt cttaatccat gcatatgaat tggttttact
     1501 cctgcaattt cagctaattt ttttattctg cttaaaatag ttcttggaga tggcggtaat
     1561 ccatcaaaag aaaaaaccaa ttctatattt ttcccaattg attgttgagt ttttttccat
     1621 gtttttaaat cttcaacggt ttgtctgtct aacgaaatta tccgaatact tgatgtattt
     1681 tttgtttcag caaattcata atcttgtctt gtacggatgt aaattgactt atttacgcta
     1741 catctgccat ttattaagtc aaagtctttc cattgtaacg ctaagagctc ctcactcctt
     1801 acacctgtaa caaaaataaa acgcatcaaa cgtttataaa attcttcata aaaatccccg
     1861 tgataaacac tattatatac tttcttaaat tcatcaactg tccaaaattc tacttgagga
     1921 cgctgagttt ttattttacc tatagtatct acaggatttt cttcaataat ttcaagtaca
     1981 attgcccttt taaaaactac tctcaactta ttgaatatct gtttaatata atttggtgat
     2041 aatggttgct caatgccctt ttttacaaca tggattttcg ataaaaactg ttgaaattcc
     2101 tgtatatgaa ttggtcgaat ctttacaacc tgcatttctc caaaaaaatc caatgcacgt
     2161 tgtaaaatat gatctgtttt catatatgtt ttttctacag ttcccaactt ataccaagga
     2221 aaaaaatact ctcgataaaa atcatgaaac gttattcctt gcagacaaat tccttgtgaa
     2281 tcagaaaaac tttgttttaa ctcttcgagt ccccttctag cttctccttg agttttgaag
     2341 cctcttctgg tcacttgaat tctttttcca aagcgatctt gtcctaaagt tgcagaaaaa
     2401 aaccaagtgc ctctttttct gtctttataa acattcgcaa ttttcctcat tttactattc
     2461 ctcccaagaa atatggagaa cttcttctaa aattgaaata ggaattcttt ttatttttga
     2521 attacgataa aaaaagtatc ctttctcaac taaaagttct ttacattcct tgaagatttt
     2581 attagcatag tactgagaaa aacctagatt aatcaaatct tttcgagtag ctgttttcat
     2641 tatagcacct tccttacttt ttttcattta tttctatcag ctgcttttgt caaaagcaca
     2701 taggaatttc acctctcccc aagtttacgg cgagccttac ggaagtatca ttatcccaac
     2761 tttaaatcat cgctttttat tgactataaa aattatttta gaatatttat cgctccttct
     2821 ttacagcaag aaatcatggc gcaactaaaa tatgctcctt aaagtgttaa cgtactgata
     2881 gaatctattt aattttcaat gaacaatagg ttttctacct aaaagaatct actaattcct
     2941 taactattag aagattcttt taaacagaaa aagtggaaag gagatttttt taattagtta
     3001 attttaaatt ttaagatcgc ttctattaat ttaatttcta gctgacttat catatattca
     3061 tctacatagg taacgaattg acctacctcg ttaaatccat ttgtcaaaca taacttagcc
     3121 atataactat gataatgttt gagtatcatc ttgatcgaaa ttggatctcc ttctagagca
     3181 gataagataa tggatagtgg aactaatagg tactgccgct ttttggattt atctaccata
     3241 ggtcataccc cattttcaat tgttcaactg ccttctttcg ttgataccag ataccacttg
     3301 tgctcatatt gagaatttta ccgatttcag catcattaaa acctgcaaaa taataaagta
     3361 ggataatctt tcttcttatc tccgataact gatcaattgc tgctgataac ttttgatctg
     3421 aaattaatat ttccattcca aataagaaat agacatcttg ttcatcaaaa tgagtttggg
     3481 aatacctgcc ttcatttgaa tctgccaatg gttcctcatt atcctttatg ttagcatttc
     3541 gttttcgaat agacttagct tcgtttcgta atactttttt acagtaggca tcaaattggt
     3601 gttgtataga tatgtcattg taatgttgtt gcatgataat atgctccttt ttcccccttt
     3661 ccactaatac aacaatcaga gagccccttt tttcgaaaaa actagaaaaa aacataaaaa
     3721 tatttatcat tctttaaaca gaaaatcttt ctataaaaaa caaaaaaacg attaggctag
     3781 gctaatcgtt ttttgaacat tcttaaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt gagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgactcgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttctt
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 tatctggcgt tgattttaga taagtaacta ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcatct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taatcaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaactctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggttc aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagagat aattccttta
     6421 cgataacctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat cgaaggtaat
     6721 ccagcttcta ttaaacctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta ataaaggaat tttgctttct gtttgtagga tatagttttg atcatatacc
     7201 tcatcagcag ttcctgggtt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaagtc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tataaatttt tgttcaatat ctacatactc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attaaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatgtcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc tttagcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 taatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatgga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttata tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa taaaaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtat atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agtttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggtgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 aattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgatc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttcttttgt taagcgtttt cattcttatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctag acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat cactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctcctctat ctccttcttt tatggataca tactttgtct agctgtacta acttaaaaag
    10441 aataaattta agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa
    10501 ataattatac gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc
    10561 atctggaatt cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa
    10621 atactcatgg cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt
    10681 cgacaacaaa ctttgttgcg ccatcgtagt caactgttga cgcattcctt cagctgtatc
    10741 attgaaccac caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg
    10801 cattgctgtt gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg
    10861 taagcaccca ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc
    10921 ttgatccccc atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga
    10981 attatttctt aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga
    11041 catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt
    11101 ttttcctgct aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata
    11161 aaaataagta ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac
    11221 tcgttggctc aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgcttg
    11281 actaagtttt aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg
    11341 acggaaagta ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgataccc
    11401 caagtcatct gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa
    11461 ttctttcggg cgataggaat ccgtttggat tttttcgtta gcacgtcgcc aaatttctgg
    11521 tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga
    11581 ccattcaaaa ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt
    11641 ttcataatca tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa
    11701 tcgccattta taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt
    11761 gtattgctta ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg
    11821 ttggtgtttt gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt
    11881 cttatctaaa aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt
    11941 tttcctctaa ataaactcca ttatataatt atttactcgc ccccttttca agcgcttccc
    12001 aaataccatt taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac
    12061 cggtttctcc ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat
    12121 cgtacattgt ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg
    12181 cttcgtaaaa atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag
    12241 gaatccgatc ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt
    12301 ctgcaatact tccagtacat aatgtaatac cattagaagg agaatccact acattacaca
    12361 accaatctaa atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg
    12421 gatcatctgg gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt
    12481 tgagaaaata tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta
    12541 ataattgctt aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag
    12601 tagaattttc tacttttcta ataatttcct ccggatcagt agggatattt tttgaaatat
    12661 aggctaacgt agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa
    12721 atactggcat aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg
    12781 tctgtttata attctcaatg tattgatctc gtgtttgtaa accaattttg atatcatcat
    12841 gaacattaac actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta
    12901 aagatttaat tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa
    12961 caattccttt cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg
    13021 ccccatacca tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt
    13081 attgattgtt ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt
    13141 tttctttcct accccaactt gatataaatc taaaccaaaa atttctttat tttctaaaat
    13201 ttcctgtacc gcacaatgaa tatttgaagg atcttgttca cctaatttca atactgataa
    13261 tttatcctgc aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc
    13321 atcgactgcc agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt
    13381 ttctgcattt ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac
    13441 tttttgagaa gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa
    13501 acgtttatta attacttcat caataaattg ttttggatca ataatgcctg gattttcaac
    13561 tactggtaaa ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg
    13621 catctctgct gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg
    13681 aagtggattc aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc
    13741 tgtcataatc acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat
    13801 aactaaataa tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt
    13861 gtgaatgata tctaaatcct cgagccccat catcaataga tcttccttta cattctctga
    13921 aggatttggc gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt
    13981 taaataagca ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt
    14041 aaaaatacta tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc
    14101 aatcggcaac tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt
    14161 atgttgagga ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc
    14221 tttagaatct tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt
    14281 aggatcttca aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata
    14341 taaactatca gccgttactg ccaaaagcct ttttttgagt tcaccatctc cactcataac
    14401 aatttccaaa atgtcattgt tgtagtcatg ataagctttt tccactactt cttcatcaaa
    14461 tgtttcacac acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat
    14521 ttctgcatga aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg
    14581 ctggccttct tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt
    14641 gaattcttcg atagaatttt gccaattttt aatcgaaacc ataattaacc tccttaaact
    14701 aatatgttag ttttgataca ttttatattt taaaacccgt tgtaaccgtt gtcaatataa
    14761 aaatggaata aacttgcttt ttttctacca tatcagaatt attgtatttt ttattcagaa
    14821 aaataaaata ctccttcttt ttggactgat tttatcgaaa cttatttttt tctttttttc
    14881 atgtattctt ttatttttat aactagtgta ttacttcaaa atttgatatt atatggcatg
    14941 aacagaatta aaatatcagc taaaataaaa gttaaggagc attgagtatg acaattaaaa
    15001 ctagtctcca aaatcaagct taccaatcta ttcgccgaca aattatctat gctgacttag
    15061 aacctggaaa aaaagtatca gaaaaaaaat tagaagaaac actttctatt ggtcgtacac
    15121 caatacgtga agcactgatt caactccgac aacatggtct tgttgataca attcctcaaa
    15181 gcggaaatta tatctcatta attgatttag attcggccaa aaatgctcga tttgtcagag
    15241 aacatttgga acgacaaatt atgttagagt gttgtgccaa aattaataac caaaaaaaac
    15301 ggattttaga aactataatt tcagaacaag aaaaagctgt tagtcaaaaa aatgctcgta
    15361 gtttttttca taatgataat ttattccata aagcttgttt tgaaattgca gagcgagaag
    15421 aagtatggca atggttagaa aataacaata cacacttaga acgatttcgt tggctacgag
    15481 taaaaacgga aggattaaag tgggaaaaaa ttatggaaca acattatcaa ctatttgagg
    15541 cattaacaaa acaagatcca gaagaagcta actttctaac atcgttgcat cttcatttaa
    15601 tgcttaatga gcaaaatatt gttttagcta ctcatccaaa atatttcaaa agtatataac
    15661 ttttaactct ttttaaatta aatgattgtt ctgatcagaa aaatatcgct tgttttctta
    15721 ttatgtaata tatttcataa actattctgc ataacaacta ttgtttgtac tttttacatt
    15781 ggataactaa ttatgactgc tgataacttt tgatattaaa ctagtatctc caatccaaat
    15841 aaaaaataga tatcttgttc atcaaaatta gttcatggat acttaccttc atttacatca
    15901 ttcagcggtt tctcattttc ttttattcta ttatttcatt ttctaataga cttggcttca
    15961 tttcgcaata cttttttaca ataggcatca aattggtgtt gtttagatat ttagttgtag
    16021 ttttgtgaca tgataagtat gctccttttt gtcttttcca ctaatacaac gatcaaaggg
    16081 tgcttttttc aaaaaactag aaaaatcaaa ataagattcg tattttgatt ctatatatcc
    16141 tgtttcaact acatcttata tttcataata gaataaatag ccttttgcat agctgcttaa
    16201 cttataagaa attgcaattt acttcttcaa agtgaaaacg aatatgagta aaataaaatt
    16261 attcttctta cagataatta aaaatagcgc gtaacaaaaa tcggcattca atcacataat
    16321 taggattgat gccgattact tatattctac gtatagtttt agtattcttt ttttatcacc
    16381 agtaaccaat tgagaacagt cctaaagcag aatagtcttt tatctcaaaa cgcttaattt
    16441 caggcttagg aatatgaata atcttttgag tatattcttt ctcttttaat cttactgaag
    16501 aaataccact gcctatcttt tttacaaata tttcatttgg tgtacagact aataaaacaa
    16561 tatttgtaat caatccttgc actaaagaat taaaaggaag actatttaaa acagggtgtg
    16621 cataactata acatgtgaag tagttatttt gaactttaac ttttaaaaac tttaaaaata
    16681 atcttatttg ttcttctaaa caatcatcta tactcatata atcactccac taattacttc
    16741 ccccaacaag aaatagcatt aatagctata ccagctgcaa tagttgcagc attgaatttt
    16801 gcaaattgtg caggagttaa acttcttttt gctacatcta aaagtatttt aacagcataa
    16861 tcataatttt tcccttgcaa cgcattaatt aaagtttgca ttagattttc acgattaagt
    16921 atatcccaaa caacacacca aatggtaatg agttcataac aacacatgca gcatactttt
    16981 gaagatcacg tttttgacgc tttcctgttg gctgcacaaa attttctaaa ataaatttcc
    17041 ctgctatacc ttcagagcca tctaattcag cttttttctg tatagcttct aaatcagtaa
    17101 ctatatattg atgtgtttct gtatcaacat aaccagcttc ttctaaatag aatttcaaac
    17161 tctctactgc ctgttgtctt tgtgtttcta tatcaggaac ttcattcact ttttcagcag
    17221 cataaacact ggttccggca ccgactaacg tacttaatcc aattaacatt acacctaata
    17281 atgaaaaaaa cttaaaaaaa cgactcattt ttatccctcc tgaaaagtat tgttgattat
    17341 caatttttat ccttttaagc tgttcaataa gacgttttta cgttccttcc gtttctattt
    17401 tattctttct gtcttataac agcttttttc aagtaaagaa cgatataatt cttcttaaaa
    17461 aacagtctat tttttcggta tataataaaa ttgtattgtt acctacatag cgaaacagta
    17521 ccaacatcat tcaaatgaag atagcaaatt tgcttcttcc tttttcttaa gtgactattt
    17581 aaataaccag aaaaaaacta aaatcacact gatatcccaa ataaattgaa ataaaggaaa
    17641 aagttttttt ctaccatgat agtttttcaa aaaatagaaa ctcgtactat ttaaaagtaa
    17701 aataactagc atcgtaaaga ctactgattt atctaaactt ctataaaaaa gtgggctaaa
    17761 taataaagta attaaaaaat gaactatttg atattgtttt ttttcatatt aacagatcgc
    17821 agctcctgcg attccatagg ctgttcctac agcaaatgga ataacggctg cacctactcc
    17881 cgtaaggctt aaacctagag cagcagctgt ataaatccca gaatgtagaa gtccagcccc
    17941 agtgaaagcc caactgcagg cacttcgttc accagtttta ttatttgttt tttgatcttt
    18001 catataattc tcaacttcat gaagctgatc ggttagccat ccatggccat tttttttgaa
    18061 tagaacgatt aaattcattt agatactgtt ggatttcaga aactgtaatt tcagaattaa
    18121 ttctatctaa acttgatttt aaatcactct catcaataat atatactaat cgatttcctt
    18181 tagaatcttt atgtgtggct aaattatttt gaatgatatt tatttcagtt tcttgttgtt
    18241 ttactaaaaa attatttgtt tcagaagcat cgacacttat tccagcgcta acaaaagtac
    18301 ttaaacctat taagaaaaca cctaaaatac caaatatttt aactattctt ttcataattt
    18361 ttcctactta aaaataagtt atttttaaaa tatcttgatt atgaattcat cccccaaagg
    18421 ccttttaaga ttgatacagc agtccgttca cttcttttag acttgtcata taagtctttt
    18481 aaatgatccc ataccagact agtttgtcta tctagcttca atccattaaa cgctaatgaa
    18541 cggttcattt tttctattac tttagataaa gctacagaaa tttttatacc ttcacaacgc
    18601 gcatcttgga tttcaataat agtatcatca attagttttt gaatttcttt cctatcaaca
    18661 tattcaatct tagaaagaga atcttttaaa gctactaatg attcaacgat ttcttttttc
    18721 tcttcagaca ttctcttcct ccttctagtg acccgtaaag ccatgtagag cagagtccat
    18781 tgaaccagta attacactat ctacaacgcc attccaattt actctacatt ttccattttt
    18841 cagatttgtc ggtccatacg gatagacttt ttgacattca atgttcggat tataccagat
    18901 aggattatac cattcagccg cagaagcaga ttcagtcgat acaaacaatc ccacaccaac
    18961 aaaaccaata cacatcacac tatagccaat taattttttc atctcgtatc catctcctaa
    19021 aaaatagaat agttcttgta gttattcata agacatattt tttccgtttt tgttttatcc
    19081 tttctgtcct ataacaactt ttttcaagta aagaatgaca tacttcttct taaaaaaaca
    19141 gtctgttttt ttctattttt tcggtgcata ataaatatga gtgttcattt tttattttaa
    19201 tacacacttc gtacataata aatcttgatc gcatcaatct tttgagcttc ttgatctact
    19261 ttcaattgaa gtaatacaaa ttgagtgacc tcattcgttg tttcttctcc aaataacaaa
    19321 tattgatccg gattctctat atcttgtgtc acctggtgaa gtttcccagt tgttttcatt
    19381 tgtacatgcg ggtctacatc aatactattg tctttaatac aacgatccgt catatagtcc
    19441 ctcacagctt gattgcgttt ataaacatct gtgaaattac tccattttct agcaaaatct
    19501 tttaagaaat tttttgtcat ttcatctatt tgttgcgctg tctcgtcttc taatgaacca
    19561 atttgactgt tactttcagt actttgaaga gttccacttg tttcttttac tgcttcgatt
    19621 gtttcttctg tcgttgtcaa ttgtgttttt tgtttttcag aagaaaacca atgacaacca
    19681 gtaagaccta tcaaacataa ccaactaaat actaaaatta ttcctttttt cacgcttcta
    19741 gacactgcct ttcttatttc accttacgaa ccagactacg aatttttgtt tgattgtacg
    19801 tgcgttgata ccgagccata atccgtcctt gctcggcatt ctgttccaca ctagagaaat
    19861 gttgtccgcc atctgaaaca tctgttataa ttccggtgtg accatagatt ccaggagaca
    19921 atgcaccacc tgcttgccaa ttcaccacat ctcctgcttg taaatcactt ggttttgggt
    19981 tcatgatcac ttgccaacca taggctgtcc aatcataatc ttcccctatt aacatcgcaa
    20041 acatgtgtcc agaacccatt aactgtgggc cacctagttg ggatacatac cacgccgtgc
    20101 caccataaca ctctccatta tttaccgatc gtccaatgat ccgatctaaa actttcgcat
    20161 tacctttact tttccctgca acaagtgacg atttttttgt tggtaggcta ttccaatgtt
    20221 tgatcgcaaa agtatcatat tctgttaagt tataagactt tatgatactc atcgctcctt
    20281 ggtaatacgg actgccttcg gtaatacctg ccgccgcata gcctccacga cctagaatca
    20341 ataaacaact cccaggatct ttattgccca cacattgatt aaagttgctt tgactcgcca
    20401 ccaaaaagcc gtaatagtca taacaatctt tcaggttcgg aaacttcata tagaagccac
    20461 cttcagaacc accaatcccc cgtgcagaac cttgtggata gggacaacca ggaaaccagg
    20521 taatcccaaa gaagtttgta tcgtttcttc cagattgtga ttgtccataa gtcgattcat
    20581 aacctaattg agaaagaatc acacttggta ataactgata ctttaccgct gcaccgactg
    20641 ctgcttgcgc atgttcatct gtgatcggac cacgtccttt aatctctgaa aaaatcggta
    20701 atccttcggt atattcccaa ttaaaagcga cagctagacc tgtagcctct cctccatcct
    20761 cggaaagttc tcctgaaaaa aagaatggca atagaaaaaa gggaagaata aagaaaagca
    20821 gacaacctag acaccctttc ttcatttctt cctcatcctt tcttcgataa tggtttttga
    20881 actgttcgtc cttttggtaa agtaaaatcg gttcctggtt ttgcgtccaa aatcggcaat
    20941 gtttcatttt tcttttctat ttttttcgat tgctctactg gattattaaa agaagaaaat
    21001 gcaggttgta aggattttgg tacggaaatc gacggtttac tttcagacga acgttgtatc
    21061 tgtcgttgca cagaacgatt tcctgataga gtggctaccg acgacttagg aacagagtat
    21121 tgattcaata actgctgcat tgctggataa ctcccatttt tcgcctgatt tttcacttgg
    21181 tgaatctgtt gtgaaagaga accagcagct gttaattgtg gcacgggtac ttttccagca
    21241 ggcaacggaa tcgattgacc aacaaaagcg atacctttat ttaacaatgg ttttctttga
    21301 ttattccctt gttgcttact cgattgtgcc aacgattgta cctgttgttt cggtcttact
    21361 gaattcatgg acgaatttgg aatcaaacca tttgaaagac cagaattcca ctctgttttt
    21421 gatttggtag aagacatttc aggctctacc ccatgataac gaacatgacg accactataa
    21481 ggcaagtctt gatttgaacg cccacctagt tcaaagggat taatcacttg cccccctttt
    21541 accgattctt gtggtgtacg tttttgttga aacttttgaa atccatgtat cgctgcagaa
    21601 gcgagtggat tcgttgctcg gtacacattc cctgctaatc ctgcaccttt gattgccaaa
    21661 ttttgcccag cagttgatag ttgaccaggc agttgtttca tttgagacaa agaatgactt
    21721 gctatgtttt tcgtgttatt tatggtttga tctgcagctc ctaaccctag tttctttaat
    21781 aaatctttcc gttttacaat gagtaaaatt gataggagtg ctttgacaaa catatttcct
    21841 aaaaaaccaa aaacagtatt cattagaatt aaatcgtcta ctatgttata gatcaaaaat
    21901 gccaccaaaa taatcacact gtaaaaagat ttttgaaaca tcaagctcaa aaaggacttt
    21961 aaggtattca tgatcatata atcaaatgct ggaacaaaag aagcgagtaa tgaaaaagga
    22021 atcacaagta ataaacacaa caccaacatt tgaaagataa aacgaatcat gccaatcact
    22081 acaagtggta atccaatact tagattaagc aatggcacca tagaaccaac gactgcttta
    22141 taaacagctt tagttggttg aatatagaca cgataagcct tctttttccc tctatcttct
    22201 cctgcagcag tggtcacttt tgtctcaata tcagataatt cttcacttga cgcttccttg
    22261 gctagaaatt cttgtggttc aatctctgct tgatacaatt cttctaaatt tgttgtccca
    22321 taatttaaca atagatacgg atcaacaaca gccgctttat aataaagttg gcgcactttt
    22381 tgaatggctt ccttttgtgt cgttggcact aaaacaccat tgacatcttt tgtcgccaat
    22441 tcatctagac tatttgtagc agaaaaaata aagccttcta cctccacgga caaatcattt
    22501 aaccctttag taaaagcttc tcctttacta ggtacgttac taccatagcc aaaccaaacc
    22561 attgaaaaaa tgacaacaaa gagaaatcga ataaacacca tttttgcgta atgtttccct
    22621 ctaaatatac gaagagtaaa tagatataac acatacaaga cgacaatccc cgcaccaata
    22681 ggatcgtata ttttcgaata aatagtatcc gaaaaagtga aaaactggtg aattaactcg
    22741 ttcattgcag ctccttcata gagcttctca atgacaaaat caaggccttg ccaaatcaat
    22801 ttacttaaac taaagaacat attcgtcatc atttttagaa aatctgtgcc atttgctcca
    22861 aataacattt tgcctaatga aaattcacta tcagataaat aagaagaaaa ggtatcaata
    22921 tgaaatttcc cgatctcata gatcgattga tccacctgtt tttctaccgc atataccaat
    22981 ccaccaacaa aaaacagtcc gaatccaacc gttaaaaaaa gccaaacgca ccatttcttt
    23041 tccttacgct ttatataagc cattgtttca cctcgtttct attgcatgat taacgtatcc
    23101 tgttcttcta tttgagagtg acttgttttt tccgttgttt gtaatgcagc ggtcatttct
    23161 tcaaacaaac tgtgtacatt gagttttcct acacgtccat agggatctaa catccaacac
    23221 tgtcctttaa tcatatggct catttcagat aagttcgctt catttacttc caaacgtaaa
    23281 tgagtcaaaa tcttttctcg ctcactgtct tcatcaaaag cgaagacaac accaaaattc
    23341 cctttttctt tatctgaacc taagtcgcca ataaattgtg tatctagcac taattgattc
    23401 atctgtgaac gccccacacg cgccattgct gcaataatac tacgtcccgc gttcgaatta
    23461 ttaaataccc atgcttctgt aaaataaatc gctgttttct tactactatc tcgttttcca
    23521 aacatttcac aaaaacgacc taaactaatc atcaaacaca aagactttcg atccgcatct
    23581 gtatatagtt ctggtcggac attgtcattt ggtaatttca aatcttggat ctctaaaatc
    23641 gttgttttgg cattaaagtc taatcccggg ttttctccat aagaaaatcc taaacgtaaa
    23701 atcgaatctt ctaccgtcaa gcgtaaaaac tcaccatatt ctcgaacctc tttttgttcc
    23761 atttgttcta aatactcgac cacatgcatc aagccaactt gttgtccggc ttctttttga
    23821 actaaaactt cttggattcc ctttaatacc gcccctttta aaaggtgatt ggtacctaac
    23881 ggacttaact catcaatcat atcctgggct acttgtttcg catctactcg ggataaacaa
    23941 acaattggat ctaatacgcc ataattacgg ggatttgtag catctaacgt gacataactc
    24001 attttttgca aatgttccac aaacaaaggg taatgtttct gataatacgg gtcttgaatg
    24061 agtttcgtaa accatttgcg cttttctcct ttgggatcca caaataaact ttgtacattc
    24121 ataaacgaac tatacaaaaa gagcaatcca actaaaaagg tcttcccttt accggttttt
    24181 ccagttactg ctacatgagg cgcatcaaac aacgcacctt tgattccttc cgcaatagct
    24241 agaagattca aaaagacaaa acgatttgac gcataaatta attcttctaa tggtgttttt
    24301 tctcctcgag aaccaccatc ccctaatgta tcttgccgac caatatacca gccggattcc
    24361 atacctaatt ctgatactgc tgaaaacaac cattcgccaa ttgcttctac agttgttcgg
    24421 tgcttccaat gcaactgtcg acccaatggt tgccctaata aacatttata gaacaattct
    24481 cgttgatcat tttttgcacg gtggatatgg atagtcgtat gaagctgttg aataatcgtt
    24541 gcactatttt gacgaacttc ttcaaccgtt tgaccgtata caataaaaca tgcgatccaa
    24601 tcgacgacag gttggccatt atctactcgt tgctttaaat gttttactaa tccccgaaca
    24661 gttaataagc gttcgctagg ttcttcatcg ttttgaacca attccttata ttctgttttg
    24721 aaccgttccg atagccaatt tagttttaaa ccaatacctc cctgtccttt caagcgtgga
    24781 tagtgaattt tcattcgtaa ttctgctggg aaagaaagat cctgtgcaaa ttgaaataga
    24841 tcacagtttc tcatattttc aggtgtttct ccaataggta acaacgctaa ataactagtc
    24901 cccaactcat cttctaattg aagaaggcct ttatgttcct tcggcatacg tacatacgtt
    24961 ccttgattgt ctttcagtac ttcccaatta ttttgttctt ctactttatg agccattcct
    25021 cgaatatatt gcagacgatt gagatacaac agctcttttt ttactaaggg acgaatagaa
    25081 aagctataaa gtaattgtgt cacttctttt acataaggat cactttcaga aaacaaagtt
    25141 tctaaatcat ctacttctaa tccaaataag cgtgctaaac ttcctgctac atctaaccag
    25201 attagattta gtacatcata aaaactctcc acggttgctt tttctttgac tttgattcct
    25261 aaaataaagg aaggacgata gacttgacct tctcgttcca acaatgtcac tgtttctttt
    25321 gcataatacg caccaattgc ttgactatcg ggagaaaatc cctttcctaa tgtgtcaaat
    25381 ttcgtccgta aattcattgt tcgtggatac atcaccaact ccacatcttc ataatctgct
    25441 aaaagtaaca gtaaacgagc catcatttct tttatttcct gtgtcttttt tggtgtgttt
    25501 tttgaaatgg ttcgtggaaa aacatgatag taggcccaga tacaatcctc gccatttaac
    25561 aaacaatttt tctttaatgc tttcactggt tcttttacaa gtaccaatag atctcacctc
    25621 ccaattaaaa aaaggaacaa ccagttgatt gtcccttttt ctaatcctct aaataaacat
    25681 acttattcgc aaaaagtaat tcttccaatg cattatttga taccttgtcc cagttataat
    25741 acacacaatc accattcatc atacgcacta cataacaaaa ccaatcccat aaataaaaat
    25801 gcactttttt tccatcaggt tttagttttg ccagtgctaa cacacataaa tagggtagcc
    25861 ctataaaaaa aacaacccct gctggtggaa aaaaagaaat aacccaacca ataaatgaac
    25921 gtaaaaataa atattcaaag attacgacca ataaaaaagt tgctgcattg actaatgcaa
    25981 ctggaaatgg aagacgccaa ttgcttgtaa tctgttgtac gcgataaggc tccgcataaa
    26041 caatcgaata atcgtaaggt tcccaatcca cttcactatc ctcctaatcc ataaataatt
    26101 tttcaatgat tttaccaaac gcatcaaata cacgcgttgg atcatttaag aagaaataga
    26161 ctgcggcacc tgctgcgata gatattaatg catttcctac ttttgatttc ataaaatttc
    26221 gcaatcccag ataaacaacc accattatta aaaaatagcc accttgcgtt aatataaaat
    26281 ctacaattcc cttcagtgtc atttccgttc gtctcctttc ctatccaacc aattatgtgt
    26341 gagtttttca acataatact gatttgtttt tttcgtaata acaagcgtca tttgttcttt
    26401 ttgtaaaagt gacgtatcac catctcgaaa gacgactgtt gcttttacaa taaaagtttt
    26461 tttcgtttct ttataacgca atactttcat ctctacttga tcaactttag ctgtctgatt
    26521 gagtccttca ggttctttca tcatgtatga catatctttt gcattgtttt tcgcatactt
    26581 atctaagaaa tcttccacaa atccttccac atctttcgct tccgacgcat caaccgtttc
    26641 gtataatgtg tcatcataag taatcattcg ttgtttttct gtattctttg gtagtgccgt
    26701 aaaataagga taagcaataa tacgacagcc cttgtttgaa atagaaaagg gaagattgag
    26761 tacttgtgtg attgtcacac ttgtttcttt cttattctct ggatccatcg agcgaatatc
    26821 ataagttact ttataactag ctgttggaat ggtttcaatt tcttttaatc caataaacga
    26881 agcagcagtt aattttcgaa tacctgaaac acttcctggt tcttgaataa agttttgtgc
    26941 ataaaactct ttttttaata tttcttgacg ttgttgttct tgttcatgat tatcgaatgt
    27001 attcatatac aaagcaacaa aacgattcat aaaggattca atttcaggtg tgtacgttaa
    27061 ttctgactga ctttgtactt ctttcttttg taattgttgg atttcatctt tcaaaaacga
    27121 cacttgaacc ttcgttttca tggattgaaa aaatgccaag ccccctgaaa aacaaagaaa
    27181 taaaaccaaa caccaaatcc atttcactgc tttttttgaa tggattttcc tttgattcgg
    27241 tactttctct gtataaactt tttctttccg ccgtagttgt cgttttttca taagctactc
    27301 cttctagtat gtttttacta gtacgtacga ttgacaacga taagcttgtt tttctgaaaa
    27361 atagtccatt caattaccct cattttctat tccttttctc tttcttttat atccaacaaa
    27421 tccagcaata cttgtcaaca ctcctcctag aacagaaagt acaacatgta tttcttcacc
    27481 agtctttggt aagacttttg tttttccatc tgctgttttg acttcaatat tgccacttgg
    27541 aagtttcttt acaggaatcg acaaatcctt taccaaattc ccttcactat cttggattaa
    27601 tggaattcca tcttttacac caacaattgt ttgtccacct tctgctttta gtggagttgt
    27661 tggtgttgga atatgggtca attcctctac attactagtg acaatcggta cactcattcc
    27721 ttgagaatta ttcgtcgtaa tttcaccgtt tggggttacc gatactgtga cttcttttgc
    27781 tggaacaata ggtttatttt cttttggaat tgcttctgat ttttcagcag gttgtttcgg
    27841 ttcttctttt ggttcttcac tggtaaacga catcgttggc tgttccgtac ttgacgaatc
    27901 ggtattcgtt tcagaagaac tggttgttcc tgattgttct ggagttgtga ctatacttga
    27961 atccgttgtt gttgaactgt gcacaccttc ttcttcaatt ggatcagtcg gcaaagttga
    28021 ttcaggaatc tctggttctg gtgccactgg ctctttttca ggtttctctg gctgcaaatg
    28081 ttcaggttct gctggttttt ccgttgttcc tccatttagc tctgtctcaa catctgataa
    28141 tccttcccct ggcaatgttt ctacaatttc tgacttaggt gtttctacag tttcatctgc
    28201 ataaaccaat gaactcccta attgaaacga aaataatgaa attcctagtg ttgttacgac
    28261 tgctaatgtt gtttttttca tgattattcc tccaattttt tatgcaattc gtgttttgtt
    28321 catagacgcc gcactaatat tatctgaacg ataaaagaac tcccgtccgt tttcttctaa
    28381 cccaacatat ggattatttt tgaataattg aatcaatagt tcactggtgt tgtattttgg
    28441 atagtttgca aaaaaataat actgtgcttg tcgcccatcc gaaaattcaa caagtaattc
    28501 ataacgatac cgcttcaatc cttttcgttg ttttaaagga tctctatacc aacttgtcca
    28561 tcgttccgtt cgtggaccga gatagacacg atacacatag aacaatgcca taccacatac
    28621 caccaaaaat aataatcgcc acataaaaat tccccctttt cttctacctt tcttgaatca
    28681 ccgaacattc ataccctcgg tagagatccg aaaaatccat cattaatttt tcgtataaac
    28741 gttgttgcaa atagcgattg tttcgaatac ataattcttc atagccatca atcacttgat
    28801 cttctttctc atagcatctt gtagctctat gacgctcagc ttgcatgaca taaccaaact
    28861 ctgcaccata tcgttctacc gttaataact gttgactacg caataatgca atataacaat
    28921 cacaaaaatg ttcagccgac atttgatttt tctcacgttt gtgaaatagg tattctttag
    28981 aaagcacatt attcgttaaa caatctgata aatagcgata atctggcggt cggttatttt
    29041 catctgtgaa tttttcccat aataaactga tttttattcg ttcagcggct agatagtctt
    29101 gttcgcgtgg ataaaattca tcaatcgtac aaagcttttt tatcacccga ataaacaaat
    29161 ccatataatg cgaaacgaac tcttcctttt caattggctc tcctaaaaat tccagtgaca
    29221 taccatcttc tccttcctta aaaaataaaa aagcagaaac cgatttggct tctacttttc
    29281 tttcctttta tagctctttt ttacggaaga atccgcaaat gacatttttt caaaaaatta
    29341 atcggcacaa gttcacgaaa acttggagca attttagata gaatcacttc cccatcttgt
    29401 aaaatacaat cataaaaatc atcagttact tgatacttac gcttcttaca ataattgata
    29461 aaatagtagc gatcattttc tatttccatt cgttgttcta ctaagcgcat acgaacatca
    29521 tcatagaaat aagccacaaa tcgcccttca agttcacgtt tttcaatggg atactctttt
    29581 ggtaacaatc tattttttcg tcgtttatac attgctggat accttagaag aagttcatcc
    29641 aaacgtctgt cagtatatgg caactgacga atcaatgatt ccaatcgttc tttatacaga
    29701 tagaataatt ggcgcaagca atagtgtact gcttctggat tttctaatac tgttggatca
    29761 cgcgaccaat acgcaccaac ataaatcata atttttttca tttagacaat cacaccagca
    29821 atactaatca ccgatccaca ataggtagaa caagtggcat agcggccgat ttcctctaat
    29881 tccagctcaa tctcttctaa agaaagatac gtctgttctt ctgttggctg gcgattcatt
    29941 tcccaagtaa cagttgccaa cgagatttct tccacaagag gtttttcgtt gctaagtgct
    30001 gccacacagt tttcgtatat cttcatttta tcgcagcctt tttttaccgc taaaatcgca
    30061 ggtaccggca atgaaaatcg ataaaagcga atgaatttta gcgttccttt tcttcggcta
    30121 aattccaact cttcgagttc ttgttcgtac cccttacgaa cttctaataa gcgtgcaatt
    30181 tcttgccagt tttctttttc ctgataacgt ccagccttta ccgcttgttc aatataagga
    30241 actcgaaaac gaaaataaga caaatcaacc atcttttcat ttaatgcttc aattgcagcc
    30301 tttaaatcca aaagacgcag ccattctgtt gtcgtatatg cattcatgca attttccctc
    30361 ctgacgttcc catccattct agatagcttt gttgctcgtc atttagttca gcattctcaa
    30421 ttccttcttg tacaaagtct cttcctaaaa tacgatctgc ttcttgtaat aactttaatg
    30481 tcggagctac ttgtttcaaa aaccagcgtt ctgaacgatc taatgagaat ttttctggct
    30541 ttacggtaaa atccaattct tccatcgaac gaaacaattt tgcccatcgt tcatcaacaa
    30601 ttcgtgcatc actttccagt cgatcccaaa caataagttt tgcattaatt aaaccaacgc
    30661 caatctctcc taaacgttta ggggaatgat ccaaaataaa cgcttctact gcttgtgttg
    30721 ctttttcatt agctaggcgt aactcatatc gattataaat gccatatttc gttaatgctt
    30781 ctatttctga aattttctca cgattcgcaa tttcaaaacg tttttcgtag aaattgaaaa
    30841 acaatggact ctttcgcgat ccaaaataca aggataaccc ttcgcttcgg aaataaccat
    30901 catctaaaaa acaaccgcca atattctgaa atttcttaaa gtccatttct aataaacctg
    30961 ctttttgttt ttctagtaat tcaaagagat caaaatgttc ttctgtttcc aatccccaaa
    31021 gttcatctaa cgcaatatcg aatcgactac attgtactcg tttataacca gcttccaact
    31081 ttccttgatt tttccaaatg gaatatagcc atgttcgcca atctgttcgt tgttcacgca
    31141 tgacttcctc atattctcgg caaccttgac ccgaaaactg tacaagtaca ttttgtgtta
    31201 cagtttgttt tgaatgccag aatacccaga tatttccacg aacagaaaga caatcaaaaa
    31261 gatacaagcc cgtcggacgt gtttcaaatt ctgttagatc cattcgtaac acacgttgaa
    31321 tcactatttt gtagtctgtc ccaaaaaagg agagacgcaa atagtcgatc attgctcgaa
    31381 gatctattcc gcttttcata tacaatcgaa agcgttcctt gattcgtttt tcgaaatcct
    31441 ctgggatttt cttttttcct tgttccatct tctttatata atctaccgag catcccaata
    31501 actgtgcaaa acgtttttga gagagtaatt ttttccctcc acctgaagcg tgaatagcac
    31561 tcaatttttt tcgataattt cgcaattcaa taggtgtcat attctacctc ctttataaaa
    31621 taacaaattt cgattttctt aatctgctag aaatcccgat ataacaggtg gtacccccta
    31681 atttttctaa aaaggagtac tccttctaac cttagctaaa gccttgatat aactgattta
    31741 aatacacttt tttatttgtg ttatgttatt tctgcccccc atattatata tcggggggct
    31801 ttacacaaag aggccgtgcg caagcgcacg acctctttgt gggcacacat gccaagcatg
    31861 tgctgccacg actggtctcg gtggcttcgc cgacacaagc cgcggcagca catactcgga
    31921 caatgtgttc tcccatagtc cctatgaaag ccaccccgat atctaaacca atgaatcttc
    31981 tttttcttgt aattctttat actgttcagg agcaaaacac tgtaaaatcg ctaaacgtaa
    32041 ctgtatgttt ttctctttgc ataaccgata tacctgacga aaacgcaata tatcctcttt
    32101 tgtcaaaaca atctgatttc tcgtaacaaa caatgtctcg cgctcaattc gtcggaaaac
    32161 ataagaacct tttgtgtagt attctacttt gttaatccac tcgtataatc ttcgaaccgc
    32221 aaaatcaggt gaaatttcac ccgcagctga agaaacacga tacaaaactt cacccatact
    32281 tcaccacttc cttctaaatt ataaaagtgt ataactttcc tcatctaccc catttgtttt
    32341 cgtatttcct tttaacgatt gcgcttgacg atcaatttgt tcttcgtaac tagtaaacgc
    32401 ctcttctttc agtacagaaa aatcttgcac aatcattgtg tcaaattttt taaattcctc
    32461 aataaagtga tagtctttgt aaacaaatgg cgcaaagaat tctacaggca catcaaaacc
    32521 aggaaaagca tacccacgac caggttgatc tttgttataa aacggcttat tcttaaaatc
    32581 ctctccaaaa gtcattaaat agccaatggg agataaacgg ccaacagaaa tacggaacgt
    32641 taaactatct cgaacaccac ttggaaaata ttccgcatct ggtcgctgca tcgctaaaat
    32701 aacatacatg cctgcttgac gaccttttaa tactaatggc tgaattaatt tatcaacttt
    32761 gtttctcgtt tccatatcca acgaaccata gaaagctgcc cattcatcaa aaaagataaa
    32821 atgcggcggt atcccataat aagcataatt tttccctggt tcatacttag gctctttcat
    32881 cgttttcata taggtatatc gctgattcat ttcttgtaac gcatttcgta agcaaataat
    32941 catttcttca ttcgttttgt aaaacacatg tcctttgaat acatcaatat cacctaatgc
    33001 cgctaaatct gccgctttcc catcgcaaat atctactgtc cctacatcta aacaagcttt
    33061 gatcaatgaa tacaataaat aggtttttcc gccaccaatc gcaccagaaa ttaatagatt
    33121 tggcgattga tcatataccc aatccaatgt tttcattaaa tgaatacgtc cattttctgc
    33181 tatacattga tcaataggaa tccgaccaga atcggaaaga aatacataat tgattcgggc
    33241 tttttctcgt tcttcacgaa ttaaatcacg caaaaatact tcggataata agctacccat
    33301 ttttaaaaat cgttcgtgga aacggccacc atctaaagga aaagaaacca ctaattcttg
    33361 tttcttttga taaaaataaa cttttggtaa tctcattttt tccttcgttt gtttctcacc
    33421 aacttgcttc tttgtataaa caaaccgatt gtcaattaac attcgagcaa gtaactgacg
    33481 ccaataaaca gaagcaaaat atccttcctt tagtcgcgac caacgcatca aacctaaaat
    33541 taatccacta accagcaaca aatacatcac aatactgatt ccaggaataa gatacatttg
    33601 ccaattcgaa tacgtattta aacgaggata aaatactttc caactaaacc aacttaatag
    33661 aatcaaaaat ggtaacagaa agaaaccata ataacaaaac cataaataac gattgaaata
    33721 acgaattcgt cgaccacgat acttaaaaaa atgaggttcc aattgtcaca ttcctccttt
    33781 ctgtttttac ttctcctgtg ctaaaaattt ttgacaaaaa gcaactaata ctggaatatc
    33841 acatttaata aaaaaccata atcgaaaaag atttaatcga tttttatatg ggtgtgcaca
    33901 catattacat attcgtacta cacgaatcca aaccacttcc tgatggtctt gtttccactg
    33961 atacggtaat gaaaaacgaa caagatcacg taaaaacata cactgcccaa taaccgcaca
    34021 aatatactca tttgtttgta taaaaatcgt tggattgtct ccaaaatact caatcaattg
    34081 ttggatattt tgacaacaaa ccataatctc tcgaagtgtt tgattggttt tgtccagatt
    34141 caaaatctcc ccttccttct accttaaata gaaagagtag gaagacttcc tcctactctt
    34201 taaaaaactt attttttctc tttttcctta gaatcagcac ttggttgttt ttgatctaaa
    34261 cctttttctt gtgattgttt cggattagaa ttatttgttg gagcttctac tttctctaat
    34321 cctttcgcat aaatcactaa atctgaatat tcacgattgc catcttgtgc tggaatatat
    34381 accacatgat attctggatc aattaatttc acaggatcac gtcgtttaaa accagtcaaa
    34441 tctacatctg ctggaaattt aactcgtcgt gcaatctttg gttcatctgg atttccaaaa
    34501 actaattgtg ctcgctgtga tcgaatttct cccgtataat cgcctgtttc attatcaaat
    34561 acacgatcgg actcttctaa tgcgccaaag cgtacggctc ctatacaagt ttcatctaat
    34621 gcttctgtaa tatcataatt atttggaaat tttaatccca tcttacaaaa cctcctcaat
    34681 ttttacacca gttaaaaaga caccgacatc ttgatagcca gttgttcgac gctctgtttg
    34741 tcccatatca tttgtttcac gactattcac tctttcttta cgaaaaacaa aataagcttc
    34801 ttcaaatgaa atacgtacct ctttttctgg taatcgtaat cttttatcct cttcagctgt
    34861 gagataaaca aaaaaagcag gctgttcttc tcgcgaacat tctgcttgta attgaacata
    34921 tcgcccagac acaccattaa tttcctttgg tggattaggt tcccgtgaac ccaataaaaa
    34981 tacctgacct aaaaactctt tcttcatacg caatgctttt tctgccatat catttttcct
    35041 cctcttaaaa ttcacaatca attctaaacg actctatatt acacaatgga aagtaatgtt
    35101 taacaacaaa tggctcatca ctaggagaca tatctactat taacatcccg tctcgttcat
    35161 gcacagaaat atcagtacac tcctcatacg tacattcttt tccactcaaa aaacaaatat
    35221 ttatattttt cttttccata ttcttttttc ctccattatt ttcttttctg taaaaaccaa
    35281 gtaataattc cttttccatc ttccaacaca aactgatgat atatttccct agcaagaatc
    35341 cacacacatg ctaaaataaa gaaaataata atactactaa agatgcactc atccttgctg
    35401 tcaaacggaa aaagatatat cataattgcc tcccccataa ttaagaaacc aattgataca
    35461 aacaaaaact ctaccatcat acaaaagaat atttcttcca actcttttac atcctttcta
    35521 actttttata aaaaaggaga ttatttttat aaacactttt attcaacaac tattttcaaa
    35581 tgaattacac attatcatct caagtatagt ttcaatcgtc actattgttt ggtccatttc
    35641 tagcttttat ttaaaacatc gctatacaag aaaaaagaaa agcactgaaa ccctaacaaa
    35701 agaaatgttt attccattta aatcttcaat tgaaaattat ctatttcaaa aaattacaag
    35761 aaaaaatatt ctagaacgaa aacaaacaat tcaacatcta atccaaacag ttgaaaataa
    35821 aaatatagac ttttatttgc cttatgaact tatttatttt gccaaagaaa tacaaaaaat
    35881 aattaacaca aataaacatt cgaaaaaaat cctcttcaaa gtacaaagaa tgtattttga
    35941 attctcatac tgctacctag cagaactaaa taaagtaaga aagcaattag gagtagctaa
    36001 acgtgatttc ttctatagaa aaaaagaaac tgtattatca cataattata ttctatctct
    36061 tttggatcct taaattctta tttattacac tcagtatttc ttggtgtatc ctcatgatca
    36121 tttactactc aacacaataa tttatttgcc tttgataaaa aattctttct aaaaactaaa
    36181 caagcggcta gttgcctaac cgcttgttcg tcctctttag tctctgactt tatacaaatc
    36241 ataaccaatc aatcctgccg caaggacaaa gatcatacca atcatgatcc ataatacatt
    36301 gtcttcagag cctgtttttg gcaacatagg taccattgca gcttgtttca cttcttttac
    36361 aggttgtggt tcgtttggtt ttactggttg cggtggtttt ggtgcctcca caaattgaac
    36421 cgtttgatct ttgtttgtta gatcgacgtt atgttttgcg acttcttcaa acgtttcttt
    36481 ttcatcttct gttttggctt ggagccattc cgcaaaactc aatccttttc ctgcaaggtt
    36541 gcgaccgtca acgatgaatt caaacaagaa ttccatcatt tctttatcag ccataaagac
    36601 tttttccgca tgtgttcctt caaatggggt gccgtctggt gttaagtagc cttgaacaat
    36661 cagtttgtaa atttgaccga ttgctaaccc attaacttga acccattcga caaccttttg
    36721 atttggtaaa gctaagatct cttttccacc atttgcattg gtagcttttg tcgtaatggt
    36781 tggactgacg attttgatgg tttggtcttt gtcggtaata tctgcgtgag aagccaataa
    36841 tacgccttct ctaaacagat cttcaaaaac tacgacttct ttgcttcgca acgcatgtgc
    36901 gtctaggata aagtctagat tgactgtacc atcagttgtt tctgccacaa acgttaaggt
    36961 acttgagact tcttttccat ctactagcac tggtttttcc gttactttgt ccattagaac
    37021 accgttgact gtgtatgttt ttccaacgat caaatcgtgg tatttcacag cgtctgtaat
    37081 ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg tagccgttgt
    37141 acgtacactt gggttcgtta ctcgtaccgt ttgacctttg tcgttgatgt ctttatggtt
    37201 tgctagttct tcgtcatttc taaaaagact ttcaaacacg accatttcac gaccatttaa
    37261 acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt tggcaataaa
    37321 gccaacagca ctttctaccg gttgaccatg aacaagtaag gcttcaccag ttgctttatc
    37381 cattaagatt cctttgacca agtaagcttt tcctggaagg atctcattaa tttcaacggt
    37441 atcatttaag gtgaacagcc ccgttgggtc tgcgatcgtc aacccgtctt caaatacagc
    37501 tgtggtatga accgttggat tgtttacccg aacactttga ttgtcatcat tgatatccac
    37561 atgtaccgca acttcaatgc catcacggaa aagtttttca aagacaacga ttgtttgacc
    37621 tttcagaaca ctagcattaa ccacaaattc aagatctttt gtgccattgg ctttgtctgc
    37681 aataaaggtc aagatacttg tgatttcttt gccttcttgt aaaattggcg cattcgttgc
    37741 cttgttcatc aatacacctg aaatagtgta ttctttgccg acaaacaggt tggtataaga
    37801 aacactatct ttaatgacga tagtttctaa tggatcaacc actttttcac catcggcttt
    37861 atttgacgct tgtgtttcta attttggtgg aatcaaatag ttacgtacag aaaccaataa
    37921 cgtttcccca tcatctggcg cattttgtct gaatgtaata gtgaatgctt gtttgcctgt
    37981 atttggttgg aaactttcta atggtacatt ttcggtaaag taatagttat tggcaaccag
    38041 accttttact aaaatctcac cattttgatc ggtggtatat ttaccaattg cttgaccttt
    38101 tccgttatat agcgtaaacg tcacaccggc aagtggtgtt ttcttcttgt tccattcttc
    38161 atacttgaac aattttacac ttttccgtgc ttgacggttc gcaaaatgga tcactttgat
    38221 cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa cgtaatttac
    38281 gggtgctttg atttcttcca aacgatagcg ctcttttcca taagtaagtg taccgttagt
    38341 taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat catttggaat
    38401 ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg tattggcaag
    38461 tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt gttcaattac
    38521 tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga cttcaagtgg
    38581 ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtagg tatcaaagac
    38641 aacagtattt ttaccaaaag tagcgtaacc atcattgtct gtcacaacgg ttttcaccac
    38701 tttgccagtt gtttttgacg tcaaagagat ttcaatgcca gctaattttg gtttgttatc
    38761 tggattggtt gaatcgacta aatctttatt agcaattttc actaaatcaa tggttccttt
    38821 gatcacttga tcttttgcgg tcgcattgac agtttttcct ggttcaacaa caatttcaat
    38881 tgtttcatca gaaagtgcat agcctttcgg cgctttgatt tcttttaaaa catatttgcc
    38941 aggatcaaga tctttaattt ctgcctgtac tttgccgttc atatttttca aggtaatttc
    39001 tttgatcttt ttcccatctg cttgatacaa gccatacacc gcattttcta aagaagctgc
    39061 gccttgtgct ttcacaccgg tttctgcatc ttctttaaag acacgaatgt ttccctgttt
    39121 caaaatagtc aaattgacaa cagagtcacc aggttcaaaa ggattcaata taccaattgt
    39181 ttgggcacct ggacgacgat acgctaatgg cgtacccaat acttcaatct gattgaaatg
    39241 aatttttgcc ttttctggtg cttcttttgt gactgtgatc catgcttcat tcccatgaat
    39301 agatacattg atcccttttg gcttttgaat gccttttaca cgttgtagaa agtcattgcc
    39361 actttttact tttaacgttt cgccaatttt tatcgtatgt gtttgattat gaaaagtagc
    39421 tcttacatca aaattattaa gcagacgttg aatttctgct tttcgacgat cataatcaac
    39481 acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa attgtgccgc
    39541 aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag agatcaagct
    39601 tgtttttttc cgtacatttg aatttttcaa atagttttcc aaagcagtag ctgtataaac
    39661 ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc cattaatctc
    39721 aaaacgaacc atataattta aagtgaagtt tggattgttc ggactagaag taacaccaat
    39781 attttcgccc caatcaattt tccgaccacc taaaacagct gtattggttg cacggcgaac
    39841 acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg tttttggaat
    39901 gtcctgctct tttttctttt gatcttcaat actagccgta gattctttgt ttagtttcgt
    39961 tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt cctcattttt
    40021 tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc gttgcttttc
    40081 ttccgcttgt ttctcagaag actctggctt cacagaaggc gttgcttcgg cagctttttc
    40141 tccttctgga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt cttcaactgg
    40201 ttgtgaagta acaggactat tccctgggtg ttgtgtttcc ccaatcgtct gatcgagagt
    40261 cgttttggaa ttaaccgttg gcgcttctgt cacaggggcc tgttcaacag tggcttcacc
    40321 agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga cttgggcaat
    40381 ggcttccacc gtaggttgtg tagccgtcgt ttcttctgca tgtgcttgag cagttcccac
    40441 caagaaacag aattgagata aggccaccaa tgcgagtgct ttcaaccaat tcattttcgt
    40501 ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa acaaaacacc
    40561 gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc gctcttatct
    40621 gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa aaaagaacct
    40681 gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttatt ggcaacttca
    40741 ccatcaccaa atatcatgcc ataccaatta ctaccttcat ccttaatttc tttccaacca
    40801 tatgacatag cttcatctaa cgaatcaaaa atcatgccac tgttgccaat tgttgcagga
    40861 tctggcttaa gatcttctgg aatagaaata tcaatcacac cataatgatc cgattcccca
    40921 gtattattgt cattactagc tactggtttg gttgttctac tgacttattt tgatacaaca
    40981 agtacgctta ttcttctttt acttctccgt tttcaatttc ccattcaaac cactccgcaa
    41041 agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct tctgacgtcg
    41101 taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc catgtttcgt
    41161 aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc tcccgaactt
    41221 gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct caaataactc
    41281 tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta acaaatcgct
    41341 acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt tccataaact
    41401 tcttcatgat cgtcccaacc aatatcactc tcgacaaatt ccacgttgtt cttatctttt
    41461 agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc acaccaccca
    41521 tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt attaaacact
    41581 aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg aatcacaact
    41641 tgttcgcttc ttctttataa aataaaaaaa ctccaataat cggaaattac tatcttttcc
    41701 tcattccttt tttaaatctt catacttctt cttatctttg tcctaaccta ataacacaaa
    41761 ttttgaagaa aaactcagta aaacctgatt ctttttttat cttcacaaca accaactttt
    41821 tcttcagatc cttttactag aacttgtata caaaaattgt tgaatgctga tacaatttgc
    41881 ggttacttga aaaaacgata gatgtaaaaa gataaataga atcatttttt tattctatat
    41941 cagtctatga ttcattgctt atcaatcacc caacccattc gatgccgttt ttttaagaga
    42001 aatttaaaaa agaccgtacg aatctcgcat tgtccgatta cttttctaga aaagatcccc
    42061 aattctgtat aaataaccct atcttcttgt tggtttcatt ttctcatata agaattcagt
    42121 caatctaccc actatgacca ttactacgtc caacacaacc gacatattaa ccacgtaagt
    42181 attccctcac agatctccga atttccatgg tatggattcc catcctgacg ctgcagagga
    42241 caacatattt catgtggctc tgtacccttt tgatttggaa agactacaaa tcaatactca
    42301 tctataactt atcaagcgaa ctttcgctag gcttctagtg attactaaat tgttggataa
    42361 catacctagc ccattttatc tagctggtat tcataacagt tatttcttgt attacttctt
    42421 ccgcttgtgt tgccattgag cttgtcaaaa agttacttag aaaaaagctg ccagtaaagc
    42481 aactttcaac aaaccctttt tgtttcttat catatcaaga aacctccttt tataaatttt
    42541 tctataataa aaaaacgtcg ataacttaat aagtatcgac gtaaactatt tttatatccc
    42601 gtaaatcaaa aattctaaag aaagatttcc tatataagca tattggataa ttcgattagt
    42661 attgggtata tctctaccat tctcatacct atctatggac cattttgaaa ttgaaggaga
    42721 aaaaagcttt ccaaactcct ccctttctag ataataatct ttcctaattt tcctcatcct
    42781 taatcccata ctaaaagaat ttatcttgtt aaaatcgcta actctttcat cttttggaag
    42841 tatattctta taatcttctc cgtacataag gtaaggaaca gtgaccttac caagataggc
    42901 aatattcatt agtttttcaa tatctggaat attaatacct ttttcccatc tacttataac
    42961 atttttgcct gtatagatgg taaagttttt accaaatttt tcaagcgtct cttcccggtt
    43021 ctttcggact tctcgtattc tttttcccat aacaatttta tcgtcttttg ataaactaaa
    43081 tatctcacta tcacgattac tctccttcga aatttctttt tcattactta atagaaaaat
    43141 atcgattttt ttttgcattt cttttgacgg atttctcttt ctatttaaca cataactgag
    43201 taatacttca ctaattccta tattatcagc taactctttg ttaagtaact tattcatatc
    43261 tctgtatgca attagttgac cgatgagaat ctcaatctta tcttcctcat cttttattag
    43321 agtttctata ttcaaaatct tgcttagagt attatctacg tctttaggga tttttcttga
    43381 ccctttttca tatttaacta aagtactttg tgaaatacct gctaaatcag ctaacttttt
    43441 ttgagatagt tttttctctt ttcttagaga tttaagaacc cttccacttg aattatcaac
    43501 catgaaaaaa gcaccttcta tgagatgctt tcatcatata ctgacagcta aaaatcttca
    43561 agaaaaaata ttccaatttt gttaatttta gtatcgccgt gataaccaaa tgacagttta
    43621 aaatctttta tacagataat cctcagtgtt gcatctactc aaaaaggttg atttaatagg
    43681 tttttcgatt gattataagt tatttcacac tatcttgctc atccattttc agaacggcca
    43741 tgaaggcttc ttgcggaact tcgacggagc cgatttgttt catccgcttc ttcccttctt
    43801 tttgtttctc taagagttta cgtttacgag aaacatctcc accataacat ttagccaaga
    43861 cgtttttacg caaggctttg atgtctgaac gggcaacgat tttttgtcca atagctgctt
    43921 ggattggcac ctcaaattgt tggcgtggga ttagtttttt cagtttttca acgattgctt
    43981 ttccacgttc ataggcaaag tctcggtgaa cgataaagct taatgcatcg actttttctc
    44041 cattcagcaa gatgtccatc ttcactaact tgcttttttg ataaccagac atttcataat
    44101 ccaatgaggc atagcctttc gtgcttgatt tcaatttgtc gaaaaagtca aagacaattt
    44161 cagaaagcgg aatattatag acgacattta ctcggtaatc atctaaataa tccatcgtaa
    44221 tgaattctcc ccgttttcgt tgagaaagtt ccattactgc accgacaaaa tcatttggga
    44281 ccatgatctg tgctttgacg aatggttctt ctacatcctg aatcgttaca ggttcaggaa
    44341 aatctgccgg gttatctact gtagcagtcg taccatccgt tttattaaca tggtaaataa
    44401 cggatggtgc agtcgtgatc aattctagat tgaattcccg ttccaagcgt tcttggacga
    44461 catccatatg aagaagtccg aggaatccac agcggaaccc aaaacctaat gcttgagagg
    44521 tttctggttc gaattgtaaa gcagcatcat tcaattgcag tttttctaaa gcttcccgca
    44581 aatcgttgta acgagaagta tcaattggat aaagaccaca ataaaccatc ggattcattt
    44641 ttcggtaacc tggtaaagct tcagcggcag gattgtctgc taacgttact gtatccccta
    44701 ctcgtgtatc ttggacagtt ttgatacttg ctgtgatata accgacatca ccgaccatca
    44761 aaaagtctct agcaactgct tttggcgaga agaccccgac ctctgtaaca tcaaatgttt
    44821 tgccattact catcagtttg attttgtctc cgggtttgac cataccgtcc gtgatccgga
    44881 catttagaac aacaccgcgg taactatcat aaatagagtc aaagatcaaa gcttttagcg
    44941 gtgcatctaa gtctccactt ggtgcaggaa catattccac gatctgctcg agaatatctt
    45001 caatcccaat ccctgatttg gcactggcta atacagcttc actagcatcg attccgatta
    45061 cgtcttcgat ttcttgacgg acacgttcgg gatctgctgc gggtaggtcg attttattga
    45121 tgactggcag gatctccaaa tcattgtcta acgctaaata aacattggcc aatgtttgtg
    45181 cttcgattcc ttgtgctgca tcgaccacta aaacggcacc ctcacaggca gcaaggcttc
    45241 gagagacttc ataggtgaag tcgacgtgcc ctggggtgtc aatcaagtga aagatatatg
    45301 tttctccatt tttcgctgtg taattgagtt ctacagcatt taatttaatc gtgattccac
    45361 gttcacgttc caaatccatc gagtctaaca gctgatcttg catttcgcga gacgtcacag
    45421 tgtgcgtcat ttctaaaata cgatcggcca atgtagattt cccatggtcg atatgagcga
    45481 tgatcgaaaa attacgaatc tgctcttggc gttctttcat tttctttata tccatagcgt
    45541 ctcctctttt catataatca gcactttcca ttataccaac gaaaagcagt aatttaaaga
    45601 gtttttaatt agtcagctat agaagttttt tttgtagaat tttgctatac tgttatcaag
    45661 ctaataaatg gagatgaaaa aatggatcaa aaagatttta gagaaaatct tgaattaaaa
    45721 ccggtcactc aagaacatat cgatcaattc aatgaattgc tttcctatgt gttccaagtg
    45781 actgaagcag acatcgagga aagcgggttt gagaataaac gagcttttat ccgttccaaa
    45841 aaaccgattt tggaagtatc gaaagtgttc ggatggttcc acgaaaataa actgatctct
    45901 caaatcgcta tctatccttg tgaagtcaat attcacggtg cacgttataa aatgggtggt
    45961 gtgactggag ttggaactta tccagaatat gctaaccatg gattgatgca agatctaatt
    46021 atcgttgcat tggataatat gcgcaagaat aagcaatgga tctcttattt gtatccttac
    46081 agtatcccct actatcggcg taaaggatgg gaaatcatgt ctgataaact ttcctttaaa
    46141 atcagagata cacagctacc taaacaaatc ccagtaagtg gtatggttga gcgattacca
    46201 gttgatcatc cagacgtatt ttccgtttat gacaaatttg cccaacaaaa ccatggtgct
    46261 ttattccgca gtgattttca ttgggaagag tattggcgtt tcgaaaatga agatgagcgc
    46321 acagccgctg tctactacga tagcaaccat gaaccaagag gtgttctgtt ctactgggta
    46381 gctgaagaaa tatttcacgt aaaagaaatg ttctatcttg atcaagaagc tcgaaatggc
    46441 ttatggaact ttatttccgc acatttttcc atgatctact gggtacatgg agatatttat
    46501 aaaaatgagc ctttagcatt tttgattgaa gatagtcaga tcaaagagca gatcgaacca
    46561 tattttatgg ctcggatcgt tgatgtaaaa gaattccttc aacggtttcc gtttgttgga
    46621 acagctgatg cttttcattt catcattgaa gatcctgtcg ctccttggaa taatggtgtc
    46681 ttcgctttaa catgggatga acagggacaa gtccgtgtct taaatgaacc tataggaaaa
    46741 tccgttcgac tggatatcca aacattgact tgcttgatga tgaattatcg ccgtgcttct
    46801 tatttggcaa gaatcgaacg gttagaaaca gatgaagaaa cactgaagtc actagagcgt
    46861 atcattccaa acatggaagc ttactttagc gattattttt gataaaaagg atgatgttta
    46921 gatgaaaatt tattttgccg caccaatgtt tgctaaaagt gatttgttat ataaccaaca
    46981 gttagttaaa gaaatcagag agctctcttc tgagctttcg atctatcttc ctcaagaaaa
    47041 tgaagcaatc aatgacaaaa ctgcttatgc agacagtcga atgattgctc ttgctgacac
    47101 agaaaaagtg ttggctagtg atttaatgat tgctttacta gatgggctga cgatcgatgc
    47161 cggggtagca tcggaaatcg gtgtggctta tgcgaaaggc atcccagtga tcggcttata
    47221 tactgatact cgacagcaag gcggaaccca cccgaaaaaa atcgctgcac tacaagaaac
    47281 agctgaaaat caatttcact acctgaactt atatacaata ggtttgatca aattgaacgg
    47341 aaaagtggtt tcttcagaaa ttgagttgct ttcagaagta aaaagatttt tagatggagg
    47401 gactttcagt gattaaagaa atcaaaaagg tgcaaatagc tttacttgct tttggtgtat
    47461 tcgttgtctg ctataatttt tatgaattta tcacacaaaa atattcgact tctcaaggaa
    47521 tcacatttat cgtggaatct ttattaggga ttgcattgat tttcatgcca caagtcattt
    47581 tgaaagtttt caaacttaaa ataccagcag caatcgtttt gttttactgg tttttcttgt
    47641 tcatctctgt ctttttaggc acagggatgc atttgatcag tatcatctct ttttgggaca
    47701 aaatcttaca tgcggtgagt ccaatggtcc tgacagctct tggctatgga ttgattggtt
    47761 acttgatgaa agatgctgag atatcaaaaa ccagtccttg gttgttcctc ttattcggct
    47821 ttgcgtttgc tggactttgc ggcgtgtttt gggagttctg ggaattctta tgcgaccagt
    47881 tcttaggcat gaatcttcaa cgttttgctg cttctgatgg cacactgttt gtcggacgag
    47941 cagcattgat ggatacgatg ggcgacttgc tgacaaatac gataggtgct gccttaatgg
    48001 gattgtttgc atggtctcaa agtaaaaaag atgagcgtta ctttgaaagt tataaattag
    48061 aaaaagtgaa aaatacctaa taaaagaggt tgtgacagaa gcgggcagct tcaagaaatt
    48121 tcggcttatt tttcgaagaa actacttctg gaacactgtt tatttgaaaa aaggaggtct
    48181 gacactagtt gtgtcagacc ccttttttct tttatagtct caatggaaga ttagacgatt
    48241 tcttctagct cttctgtttt gaaatgaagt tgtccatcaa gtaaagtaat cgttactttt
    48301 gatttaggca taacatgtcc tgcaacgatt tctttagcta gtggtgtttc tacttcctta
    48361 gtgataaatc gtttcaatgg tcttgcacca tatgctggtt cataagcatt ttcagcaatc
    48421 cacgttttgg cttcatcact gattgtcagc aggatttctt gatgttccag acgttgtgcc
    48481 aattgcgcca ccattttgtc aacgattcct ttcacgttgt ctaggctcaa tggtgtaaat
    48541 aaaatcgtgt cgtcgattcg gttcaagaat tctggtttga agtttccacg taatagcgtg
    48601 ttgacttgtt cagccactgc ttctggaatc gttccatcag cagtcacgcc ttccagcagt
    48661 aactgtgaac cgatattgct ggtcatgatc agaaccgtat ttttgaaatc tactactcgc
    48721 ccttttgaat cagtcaagcg tccgtcatct aggacttgta acaagatatt gaagacatcg
    48781 ggatgcgctt tttcgatttc atccaacaag acgattgtat aaggatttcg tctgacagct
    48841 tccgttaatt gaccaccttc ttcgtaacct acatagcctg gaggagctcc gactagacga
    48901 gacacagcat gtttttccat atattcactc atatcgatcc gtaccatatg atcttctgag
    48961 tcaaacaagt tctcagctaa cgctttcgct aattcggttt ttccgacacc agttggtcct
    49021 aggaaaagga aagaacctag aggacgattt gggtcttgca atccagcccg tgaacgaatt
    49081 accgcgtcac tgaccgcatc aactgcttca tcttgaccaa tcactcgttt atgaagtgtt
    49141 tcattcaatt taattagttt ttctcgttca ccttctacaa gtttcgttac ggggataccc
    49201 gttaatctgc caacgacttg agcaatctca ttttcggtaa cggactcttg aaccatctta
    49261 atctcgctgt cttttgcttt agcttctaat tccttcaatt ctttttctaa ttgaggtatc
    49321 gtaccatgac gcaatacagc agcgcgttct aaatcatagt tattttctgc atcttctaat
    49381 tcgtgttttg ctttatcgat ttcagcacgt tttgctgaaa cagaattgac ttcttctttt
    49441 tctgtttccc actgcatctt catcgcattt gcttcttcac gaagttcagc taattcttct
    49501 tgcaaatttt tcaagcgttt tttacttgca tcatctgatt cttttttcag tgcagcttct
    49561 tcgatttcca actgcatcaa acgtctagta acttgatcta attccgttgg catagagttc
    49621 atttctaccc gaatcgttgc actggcttca tcgatcaaat cgattgcttt gtccggtaag
    49681 aaccgatcag taatataacg gtcagataaa gtagctgctg ctactaatgc gttgtcatgg
    49741 atgttaacac catggtggat ctcaaaacgt tcttttaatc cacgcagaat actgatcgta
    49801 tcttccactg ttggttcttt gactaatacc ttttgaaagc gacgttccaa cgctttgtct
    49861 ttttccatat attgacggta ctcatctaaa gttgtggcac cgatcaaatg taattcaccg
    49921 cgagcaagca ttggtttcaa cagatttccg gcatccatac ttccttctgt ttttcctgcc
    49981 cctacgatat tgtggatttc atcgatgaac aagaggatac gtccatcact ttttttgact
    50041 tctttcaaga ctgcttttag tctttcttca aattcaccac ggaattttgc tccagcgatc
    50101 aatgcaccca tatctaatga aaaaatcgtt ttatctttta aattttcagg cacatctttg
    50161 cgtacgatac gttgagcaag cccttcaacg attgccgttt tccctacacc gggttctcct
    50221 atcagaacag gattattttt tgtttttcgt gaaagaatac ggatgacatc acggatttct
    50281 tcatcacgac cgatgattgg atccattttt ccgcttttga cttgctgtac taaatcaacg
    50341 ccgtattttt ctaatgcttt gtattgttct tcttggtttt gtgaggtcac tctttctcct
    50401 cctctcatct cttcgatgtt tttacgcaat tctttttcag ataatccgtt tttattcaaa
    50461 taaacagtta acggataatt ttttaatttc atcaacgcta aaataacgac ttccgttgaa
    50521 agaaactcgt cgccgaagct ttcacgtatt tggtctgctt cattcaataa tcgaaagaaa
    50581 ttctggctga ggttttgtcc atattggacg tttccaccag aaacaactgg atactcatcc
    50641 agtagacgat caatttcatg ttcaaaagat tcgacatcca atccggcatc cgtatagaaa
    50701 tttcgaccaa aatgattagg ttgtaaaaag attttccata catgggcaat gtcgatatct
    50761 tgatgttttc gagtgaccgc aatctgttga gcttcagcaa tcgcttcttg aagcgtcgta
    50821 gtcattttct caatattcat acaggtatac cctcctaatt aaaggtaaga tcacttattt
    50881 ctttgatctt tacaaaaact attatatcgc tttggtcaat tttggtcaaa atatacacat
    50941 ggttttttga ctttttttct cgacttatat aaatcttaaa aaaacaaaat tgattgtatg
    51001 tatataaaaa aagagccaaa caaacgtaaa attatcttat ccataacgtt tttctttgat
    51061 ttgataaaaa gggtagatct tataaaagac ctacccttga ttttctttat tctctttttt
    51121 tacttctatt atttcttctt tgatcactct tcgccgttca gcaatcacat acacaaatcc
    51181 atagagaaat ggcaagataa aggaactgaa acgatacagt aaaagcgaag aaactgcgtc
    51241 aactgttcca acaactggtc tgaataataa caaatagaca aattcaaagg aaccaatacc
    51301 agcaggcgta ggaataaccc ctgaaagcac tactgcaaag ctgataaacg aaatggtcag
    51361 tagaaaatct attttaggat ggttttcaat cagaatcaaa taaggaagta cataccaaaa
    51421 tacaagtttt aacatattcc atagataaat ccgcaatagc gcagtcctgt cttggataat
    51481 ggtttggacc gtttccctta aagagtagat ctgctgattg catgtatcca cccatttccg
    51541 caaacgctca cttttaaata acttattcga taagctgaca aaaaatattt gaacattcaa
    51601 gctgacggac atcgccaata aaaaggcaac aatcacgatc gtcaaaagga taccagcgat
    51661 gataaaccag atcattgatg gaccattaga ataaaaaaga gagaattgga tgaccaagcc
    51721 agctaatgca taagtgatca ctgccagttt gtacataatc atatggagtg ctgtcacccc
    51781 tgctccctgg gatagcttca ttcctttttt gttataaaaa taaatctccg agatcagcgt
    51841 tcctgtacca aacgaaacga ttcggtaaaa cgcaatatag ctagaggtca agaacccatc
    51901 tttcgttgta aaattcggcg cgaaaggttc agcaatttct ttgattgagc gcccttcagc
    51961 gatttggtaa actgtcccta atataacagc cgctgccaaa acaacaacac tggtagacag
    52021 caattcggca aagatatctg caaatgattg attgattaaa taataaataa tggcaaaaat
    52081 cacaatcagc aaaagcagat tcagaatgat tttagttttc gttcctttag acgacatctc
    52141 ctgctcctta atggaaaaaa taaatgagaa tcaaatcaac aagtacacct gcagcgaatc
    52201 ctatagtaaa cttctttttc cgagttttat gatggaaaac ttttcgagag atcaatcctc
    52261 ccaaaccacc gcctaacagg ctgacaaata tcaaattatt ctcaggaaca cgccagccgc
    52321 tagttctagc ctgatgttta tcatacccca tcagacaaaa taagtaaaaa ttcacaatga
    52381 aaaggtaaaa ccacaaaaga ttgtgtaaca atacatccat tactcttctt ccaactttct
    52441 gttaactact atttctaagc tttttcttct ttctttggtg aataaactgt taatcctttt
    52501 gatacaacag ataattcaac tggaagatca tctgtcgtgt ctccatccgt tcgagtgccc
    52561 acagattttt cctgcgcttg gattttcatg cgtttggctg tgaaataagt aatgccgggt
    52621 atcgtataga atttgcctct catgattcta ggcaaataaa aaataaatcg gaaaatatct
    52681 aaggaaggta cgatcgttac atgaaatttt ccatcatcta gggtcgctga gtcgtcaaaa
    52741 tttgtaaaac cgcccacgga gttagacatc gtgactgtca acagttggga ttttcctttc
    52801 caatgctcct cgtctgtttc taaatccaaa tgatattttt tcttcttcag caataactta
    52861 aaaaacttct tgataaagat ccacgagccg taacgctgtt tttcttcttg actgatccaa
    52921 actgctgtat ctgctaagat tccgattgtc agtgtagaga taatgacttc atcattgact
    52981 tgaccatagt ctattttccg tacattgcct tccaggatca cgtcagcagc ttcttctttt
    53041 ttgaggggaa tctcaagaac acgtgcaagg ttgttgacag ttccacctgg aagtaatccg
    53101 acatggtatt gagataatgt tttttggaac gcttggacga tatgatgaat cgtgccgtct
    53161 ccacctataa ttactaaagt atcagcttct atggcttctg ctttcttgac taattccgca
    53221 tcatcaatcg atgggccagt ttcagctagt tctacatata attctggctg cttttctttt
    53281 acataatctt gaaaccagcg tgctaatttc tcaccctcat ctttccctga acttttgtta
    53341 taaaaaacta gcagcttttc cattctttac ctcctattta ctcttcaaaa aagagggcgg
    53401 gacaaccaat tccattgtcc tgccctttag ccttagacag atgacattcc atcattggga
    53461 cgaacgcctt gtcataagct gttctttttc gattttaact ctttgtgttc aattctgcaa
    53521 tttttacaat cacgtctgtt gctttttcca ttacttgtaa agaaacaaat tcgaatcgtc
    53581 cgtgcatatt ttctcctccg gcaaatagat ttggcgtcgg aatacccatg taagagattt
    53641 ttgaaccatc tgttcctcca cgaactggtt cgatcactgg agagatacca agctcgatca
    53701 ttgcttgttt cgctaattcc acgatgctca tatctttttc aatgatttct ttcatgttgt
    53761 aatattgatc atacatatct actttgattc gttcttgatc aaatcgctga ttcaactctt
    53821 cttggatatg tgtgatttgt gctttgcgtg cttcgaattt ttcacgatca tgatcgcgaa
    53881 tgatataact catagaagct tcttcaggat tcccatccaa tgccatcaaa tggaagaaac
    53941 cttcgtatcc ttccgttttt tctggaactt catcggctgg aagctgatta tgaaaatcga
    54001 ttgctaactg tagagcattg atcattgtat cttttgccgt ccctggatgt acatttttcc
    54061 cttggatcgt aatatttgct tgagcagcac taaacgtttc atactgcaac tcacctaaag
    54121 gaccaccatc gactgtataa gcaaaatcta catcgaattg ctcaacatcg aatttatccg
    54181 ctccgacacc gatttcttca tcaggaccaa aagccacttt gatggtaccg tgagggatag
    54241 aaggattatt gatcaagatc tccattgcag tcatgatttc cgcaatccct gatttgtcat
    54301 ctgctcccag taatgtactg ccatctgttg tgatcaatgt gtggccttca taatttttca
    54361 aatttggaaa atcttttgta ttcaatgtat agcgttcttc cgcatctagt acgatcgtag
    54421 actcgccatc atagttttca atgatttgtg gattgactcc tactgcatta aaatctgctg
    54481 tatccatgtg ggaaataaat ccaatggaac gcacttcatg atcaacattg cttggtaatg
    54541 ttcccataac gaatccgtta tcttcattat agaaaacatc agctaagcct aactcttcta
    54601 attctttttt caattcttgc gcaaaagcaa cttgtgtctg agtcgatgga gtcgttttgc
    54661 ttgttgcatc ggagcgtgtt tctgttttga catatcgtaa aaaacgtggc aaaagatttt
    54721 cgtacatagt attctctcct tttaattaaa ttgaaatggg tttgtatttg tttcagaaac
    54781 gaaaaagtta atatcccaat tttcttcttg tttccaagat tcaaatttct cgataaatcg
    54841 ttgcttgcat aacgattcaa tataatgacc gggatcaatt gcaatcaacc cagcgctttg
    54901 catatcatga gccgtatgat aatagatatc tcccgtgata tagacatctg ctctttgggc
    54961 gattgcttgc ggataaaatt tttcaccgct tccaccgcaa atcgcaatgc gcttcacaga
    55021 ggattttgca ttttttggct ggactattct cagtccatct agttggaacg cttcttttac
    55081 ttgttctaca aatgcctcta tagaaatttc ttgcggcaat tcacctactc gtcctaaccc
    55141 aaacatttcg actggttcat ctatagcgaa aagatcataa gcaggttcct cataaggatg
    55201 agctgaccgc atggcttgaa tcacttgttt ttcgatagtt tccggcaaaa taacctcaac
    55261 ttttgcctct tgaacttgtt ctgtcttccc tactttccca atcgctggtt gtgcaccagc
    55321 ttccggagta aatcgaccgt gtccaatcga ggtaaaactt gtaccggtat aatcaccttg
    55381 tgtaccagca ccagctgcag ctaatacttc acgcattttc tgtgcatgat ccacaggtac
    55441 ataaacggcc agttttttat aatgaatctc atgagtcttt actaaataac tctccacttc
    55501 aataccgaga agttcacaaa accagtcatt cagtccatcc caaataatat ccatgttcgt
    55561 gtgtgctgca tagacggcaa tatcatgttt caacaaatct gcatacattt tttcttgtgg
    55621 ctgatcagta accagccgtt ttacaggacg aaagattgga ggatgtttag caatcagcaa
    55681 atcgattttt ttcttgattg cttcttcgac cacttctgga cgaacgtcta atgtcatcat
    55741 tactcgttgg attggcttat ccaatgttcc gatatgcagt ccgactgggt ctccttcttc
    55801 agctagccac tgaggacaat agctttcaaa cttttggatg aaagtgcgtc cgctgattgt
    55861 catggcttta aaacctcctc aatcatttgg atttcctgct cgatttgttt ttgacgtgcc
    55921 tcttgattag ctgcttgttt gagctgttca cggatagctt ttctttgttg aagctctctt
    55981 gtccattttg atttgaagat ctcacttttc tcttcaagca gaaatggccc gaacatcagc
    56041 tccatttcat tgtaagtaac tggtttttgt tctttttcgg ctacaatgat ttcatatatt
    56101 tttttatttt cttcaagtat tttttctgat tggattttat actgattttc ttgcagccaa
    56161 gatcgtaatg tacgctctcc gatgtttggc tgaaggatca aacgttcttg tccgcttaag
    56221 cgttgattca acttgccagc ttctaggata tctcggatca gtgaacctcc catacccgca
    56281 atcgtgacag ctgtaatacc atcttgtggc tggatagcat ctagaccatt tgcaaggcgt
    56341 acggtgatac ggtcttctaa tccatctttt ttgacttgct tacgtgctga ttcataagga
    56401 ccttgaacga cttcaccagc aactgcatat tcgatttttc ctcttaatat caatgcaacg
    56461 ggtaagtaag cgtgatctga tccaatgtct gctaaacgag cggattcagg gacaaagtca
    56521 cccactgcgg ctaagcgctg agataaatct aaatggttca tagcttcact ccttctagat
    56581 aattatagca ttttccaaat aaaataagga gactaggaat aaaaaaattc ccatatctcc
    56641 ttaaaaaata taccttttcg ttctataaaa caacattttc ttatttttga aaaaatgtta
    56701 ttggttattt gttcaatgaa acatcaataa tcgaatctcc aggaacttgc ggtgggatag
    56761 acatcgtcat gatcggcatc tcaactaatg taaattggac tttgtcaccg attttcaatt
    56821 catttatgtc cacgcttatt ttttcagcag tagcatttaa gatcactccg tcattgctga
    56881 atgacgttcc catattcgct ggatcttctt tggccatgac atcgacgagt ttcaactgaa
    56941 gcgtttcttc ttctgcttct acttgagtaa tactttcaat cgttccgaca aatatgcttt
    57001 gtctctccgt tttcgtactg cttgttttta ctgtttgacc cgcatcaatc gaatcaacag
    57061 aacctgtgtt gctatctgcg ctaccgcagc ctgctaaaag aaaaaataga ccaactacag
    57121 atatactaga aaaaatagcc ctcataaaaa atcctccgtt taagtttctg cattacttgt
    57181 tgcggtgatt gtctccggtc atttctgtcc atataatgat tcgctgacct gcttttacta
    57241 ctaggtcaaa tgtcagttgg acttgtcaac tgttcaacca ttgcgtcggt tgcgaccagt
    57301 catttcgatt tcttttgtca aataacgtat gcgttccatt atccgtttcg cttttagcgg
    57361 ctcttcatct ccacgttgag tgacggacaa atgctgttcc aattcatcca ttgtaaaatt
    57421 cgatgagaag aaggttggta actgttcctg catccgatat tgaaggataa caccgaatac
    57481 ttcgtctcgt atccaactac tcatcgcatc tgcaccaatg tcatcgatca tcaaaatagg
    57541 tgaacgtttc actgcatcta acttttctgc cacttgatct tttccgattg cctgtttcat
    57601 ctcaacggca aatgtcggaa aatggactag cgtcgtggtg aatccagcaa tcgctaattc
    57661 tctcgctgtt gctcctaata aatatgtttt tccaatacca aaacttccta ctagatacag
    57721 tcctttatga tacccttttg gatcagcagt atatttctct acaaaatcta gggcttctaa
    57781 gctcgcatat cctcgtccat ctgtacgttc aaagttttca aaagaagccg attgaatatc
    57841 ttttggcata tccattgctt ttactcggct tctgatttct tcttgtcttt gccgtgctaa
    57901 caatgcttcc gttggtacat aagtgacatc cacataatga aaattcaacg tcagcttcgg
    57961 ctcatagccg ggagcgatca tcgttggatc gtttttttga tatttacgct tttcttgcac
    58021 gaattcatac aatttagcgt aacttttttc aatatccgct tgtgtcaatt cttcttggtg
    58081 tgcgcttaga aaagcttgaa catccgcatc atttaagact tcagcgatca tttcagcata
    58141 cttttcttga taattgcgtt tgctgatgat cctcttcaat tctttgccta cgtcttccac
    58201 tagatatcac cctccttttt tgccaggtat tctttaaaac ggcggtcaat ctctgctttt
    58261 ttctcgctgc tgatctgttt ttcatctttt ggctggttga cccaatcagg aagcttttcc
    58321 cgtcgaacat ttcgcttgcc agtttgataa tttgtttgct tcttttgttt tgattgtttt
    58381 gcttgattac ttatttgccg aatgtgctgg atcgcttctt ctgctgtggc gattttcttt
    58441 tgtgcccatt catttgccac tgtatttaca taactggcat ttaagctagc attattcttg
    58501 atcactagta cataattcag caaaacattg atcacgctac tggaaagacc tgattgagaa
    58561 acaagatctt gcagtaacca gttttcttgt ttagaaacat atccgccttt ttctgacttg
    58621 attgcttcca gatatttcat cggtggaatg ctctgtgctt cttttatcag ctgaagcgct
    58681 gcgtttgtca aatgttcgtt tgacgagggt tcattactgt tttcagaaac cgcaactgtt
    58741 tcttttgttt gcttcaccgg acgtctctgc attgccaaaa tggctttttg cagttctttt
    58801 tcagatactt tttgttcact aaagtcataa gattgactga tcagttcgcc aagtgtcaat
    58861 tcatcgaaac cgtaaagctg atgatacatg tcgacttgtt tgatgattgg atcatcaaga
    58921 cttgcaatat gtttttttgc cagccaatca ttcaaaaact gccaatcgaa tgtcgaatct
    58981 gcttcaagta atgctactga ttcagattct tcaaaagatt tattcagcga ttcgatgttt
    59041 tcttgattag cagcgtacac tgcttcattg aagcgataaa catctaaaaa tttttttgtg
    59101 attttttgat atccctctgc tgtatgcctt ttaggttcaa agaattgggt aagcttctgg
    59161 aatcgtcgtt caccaacttt ttccagaaga agaaaagcca gaacagagtc tttgaaaaat
    59221 gctttaggaa tgagtggttc taacagctca taaataaaac gaggtcccag atcactatct
    59281 tctttaaaat aaacatccag taagccgatt ccttctagct tttttcttgc atcataaaat
    59341 tgcgggatac cgcagcctag actactaagc agatctgcat gcatcccttc aggacctttg
    59401 ccagattctt gagagatttc tgataacagg gtgaaataga tactcaaggc ttctccgccg
    59461 attagtggct gataaagata cagcaaggcg ttcttgctgt cttctctcag cggagccgct
    59521 tttcttcctt ggtaaatatt ttttggttga agctctttcc aagcattttc caaggaaatg
    59581 cctccttttt gtttcttaat tgttttcttt attattctct ttattatttt cttttgcttt
    59641 atccacgata tcttgcaact cttttaagaa aacagacata tctttgaact ggcgataaac
    59701 acttgcaaaa cgaatataag cgatttcgtc taaatcaact aaatcttcca tcacatattc
    59761 accaattaga ttcgtcgata cttcgttttc tccgagactg cgtacgcggt tttcgacatg
    59821 gttgacgatt tgttccattt gttccatagc tacaggtctt ttttctgctg aacggatgag
    59881 tcctcttaaa attttatcgc gattgaactc ttcgcgttcc ccgtttttct tgattactaa
    59941 taacggagca gcttcaattc gttcaaatgt agtgaatcgg aacgaacaat tctcacattc
    60001 gcgacggcga cggatcgcac gtccatcatc tgcttgacgg ctgtcgatta ctcgtgaatt
    60061 attatgttga catctgggac atctcatttt gtctcacctc ttgcatcgca ttttatataa
    60121 aggatgtgtc tttctttaca actagttgta ttataacacg aaacaaatca caaacgcgac
    60181 tgtcctaccc aaaaaacacg gatataagaa caattttttc tactttcatg cgtaattgtg
    60241 tcccctgtcc ttactaaatt tcaattttcc aatactgatc accttgatta ctgagaaata
    60301 acttacacaa aagctgattt agtcatctag cacaaaaaac ttgaagcaga tgaacacctt
    60361 ctcatctgct tcaagtcaca tctcgatttt ttactatttt ataacttctc ttcgattcct
    60421 gtatagtgtt tcattcgctg ttcctcgatt ttttgctcat cggaaatgtg gtcactttct
    60481 gggagcggtg ttttactacg atcaggtgtc ggattttttc gttcttgagt caaatcttgc
    60541 cgcttcgctt cttcttctgc ctgctggtct tctatttcat cgattcttgc ttggtactgc
    60601 ttttcttttt cttctgctgt ttctgattgc gtacgacctt catcgattag attttcttta
    60661 ttgagcatct cgccgatttt ttcttttgcg ttacctacga ctttatcctt ttgatttttt
    60721 aatgatccca tcgctatccc tcactttcta tatactcagg atagcaaaat tgacacagat
    60781 actcaaagaa agcgattagc aaaaagccaa ttttcgattt gtcccacaag tttttctttt
    60841 gttccattgt tgtcgaacaa aatatccgca cgctgctttt tttcttcgat tggaagttga
    60901 ctactaattc tctgctgcgc ttgttctagc gtcagttgat tacgtttcat caaacgctga
    60961 agctgagttt ctggcgtagt ataaacgaca gctactgcat ccatatagtg gtcataatga
    61021 ccttcgtaga gcaatgggat atctacaatt actaaaggat gattggaact tagagaggcg
    61081 atttgatctt ttatcgcttt tcggataaac ggatctaata gctgatcaag tttttgccgt
    61141 ttttcctttg atggaaaaac aatcatccca agtttccctc gatctagttg cccatcaggc
    61201 tgcaatacct cttggccaaa cactgataca attttcctca gagcaagcgt tccaggctca
    61261 acagtttctc tggcaatcac atcgccatcg atcaaaggaa atccatgaga ctgaaagact
    61321 ttggctgccg tacttttacc ggtagctatt ccacccgtca aacctaaaac aaatcctact
    61381 ttgcgatttt tagcctttga caattcggac aatagtgcgt ccccctttgt gccacttttg
    61441 ttttcacgat cggcgttcca caacgtatac atggtttgcc agtttgctga tagacatgca
    61501 gtgctacttg aaatccgcct gattctccta atgcattcaa ataactgcga atcgtggtac
    61561 ctccggcttc tattgcacga tccaaaacat caatgatcga tagacgtaat tgttctacct
    61621 ctttggggcg cagtgtgttc gccggttgtt ctggatggat cttcgcttcc cataatgctt
    61681 catctacata aatattacct agaccagtca ctagacgttg gtctagcagc aatggcttga
    61741 ttgctttgtg ggactttttc agtccatttg cgaaatctgc taataagaag ctatctggca
    61801 aaggctcagg tcccaatttc atgattcctc ggtaagttgc acttgctcct ttttcgacga
    61861 ttgtcatacg accaaatttt cgtacatcgt tgtagcgaag ttggcttcca tcctcaaaaa
    61921 agaaaagtac atgcgtatgt ttatcgattg gtgtattttc tttcgtaaat tgatattttc
    61981 cttccattcg caagtgtgag atcagttcac aatgatcgag atgaaaaatt aaaaacttcc
    62041 ctctacgtcc aatggattgg atggtttcgc caacaagaga agcctcaaag atgggtgtct
    62101 ctggttgctc gatgatcttg ggccacaaga cctgaacttt ttgaatcgtt tttcctacta
    62161 ctaaccgttc caatccttta cgaacggttt ctacttctgg taattctggc attttttctt
    62221 ttcaccttca ttatattgct ctgtcttaaa caatgtttat tttgcttcgt accacgtttt
    62281 tccccaactg ctgtcagtga tcagtggtac atgaagagaa acagcttgtt ccataacttc
    62341 cttcaccaat ttatctaatg attcaagttc tttttccggt acctcgaaga caagttcatc
    62401 atgaacttgt aacagcatcg tagcttgaag acctgtttct tttaatcgtt tatccaattc
    62461 gatcatcgcg attttcaaaa tatcagcagc acttccttgg attggtgtat tgatagctgt
    62521 tcgttccgca aaagaacgga tattgtaatt tcttgaattg atgtctggca gatatcgacg
    62581 gcggtgatac agcgtttcga cgtagccttg gtcttttgct tcgcgtacga ttctttccat
    62641 atattctttg acacctgggt atttttcaaa gtacgtatcg atgtattgct gtgctgcttt
    62701 tcggctgatt cctagatttt gtgaaagtcc gtaatcagag atgccataca cgataccaaa
    62761 attcactgct tttgcctgcc tacgcatatt tggtgtgaca tcctctgcct tttcgattcc
    62821 aaatacacgc atcgctgtgc tggcatggat atcttgacct tctaggaagg cagctttcaa
    62881 atgttcatca tttgagatat gcgcaagtac gcgtaactcg atttgtgaat agtcagaaga
    62941 ataaatcagc cagtcttttt ctctaggaac aaatgcctga cgtatctttc tcccttcatc
    63001 taatcgaatc gggatgttct gcaaattagg atcaacagag cttaatcgtc ctgtttgggt
    63061 caatgtctgg acataacggg tatgcacttt tccatctgat tggatcactt tcagcagtcc
    63121 ctcgacatag gtagattgaa tttttgcaat ttgacgatag gtgaggatat cctctacgat
    63181 tggtgcttgt tcacgaagct gttccagtac atctacggct gtggaatatc cagtttttgt
    63241 ttttttgata acgggtaatc ccattttttc aaataagatc actcctaatt gctttggtga
    63301 attcaaattg aattcttcgc cggcttcttc gtagattttt tgttcgatct ctcttaagcg
    63361 agcagagaac tcgcccttca tttcttgtaa gcgagtcgca tctactttga tcccgcttat
    63421 ttccatctct gccaaaatga tagacaatgg cagttccatt ttccggaaaa gatcttcttg
    63481 gtttttatct gccagttctt ggctcaattt tggtgtcaac gcattgattg cagctacttt
    63541 tcgcgctaaa tgagcaaaaa agagttcctc ttcttctggt aaaccttttt tcgccccttt
    63601 gccgtataca gcttcatctg attggatatc gttgtatccg taatgagcag ctattccttc
    63661 gatatctgtg cttttttcat tcgtatcgag aaggtatgca cctagcaaga catcaaaatc
    63721 gattcctttc gtttttccag tatagcggtt taaagcaaca taagttcgtt tcgcatcata
    63781 tacttttttc agccgagtcg gatcagtgat ccaagaatgg aacgctcggt tttcgaatac
    63841 agatagatcg ttcgtgacat aaattttctt ttctgtcccc cacgctactc cgacgatttc
    63901 ctctgtatga taattgtcgc ccatcatttc cacgtacaac gccatatccg tagtaaacat
    63961 ttcctctttg aattcatgaa cgacttcaaa caaaatatca tccattttta cttcttcttc
    64021 tacgatattc aatttagaaa ggaactgatt gaaatccatt tctttataaa acggaacgag
    64081 tttatcgaga tttttccctt catacttcaa tgagtcgata gagacatcca caggcgctga
    64141 agtattgatg gtcgccaatt ttttcgataa gaaagcctgc tctttgtcat ttatcagatt
    64201 ttctttcatt ttgctttgct tcatttcatc gatatgttca taaattcctt cgactgagcc
    64261 atattgtttc aatagcttga tagctgtttt ttcaccgatt ttagtcacac cggggatatt
    64321 gtcagaggca tctccggcta gtcccttcat gtcaataatc tgttttggcg tcaacccatc
    64381 atatttttcc gctacatgtt ctggtgtata gctttcgata tcgctgacac cttttacagt
    64441 gatatccact tttacttctt tactagctaa ttgagtcaaa tcccgatcac ctgaaagcac
    64501 gacgacatca aattgatctt tgtctacttt ttctgccaaa gtaccgataa tatcatctgc
    64561 ttcataattg ggcaattcgt aatactgtac ccctagtcct gtcaacaaat cacgtatata
    64621 tggcatttgt tctttgaatt cgccaggtgt tttggatctt ccacctttgt actccgcata
    64681 catttctgtc cgaaaagtcg tttttcctgc atcgaacgca accagaacat gagtcggttg
    64741 ctctttttgc ataacatttt caaacatcgt attgaatgca tagattgcat ttgtatgaag
    64801 accgtttcta tttttgaaac gttcaagaga attgtgaagc gcaaagaatg cgcgaaaagc
    64861 cacactgttc ccatcaacga gtaataattt attttttgtc atcttcttaa ctccttactg
    64921 taaagttctt attcattcta acaaagaatc ctgcggaatg cgaaaagaaa aagcgggacc
    64981 ataccaaata attcggtata gccccaactg atgaattttt ttctttttta gttgttccgt
    65041 ccaaagattc tcagaaacgc caagaacaag ttgataaagt ccaaatacag cttcaatgcc
    65101 atgaatacag caattccact atgtgctgtt ccgtttgtct ggacatagat attacggatg
    65161 ctttggttat cataagctgt aatccctgca aagatgacga ctaacagtag ggagatgaac
    65221 agctcaacta cgctacttct caaaatgaag atgttcaaga ctagtgcaat gatcactccg
    65281 attaaagctg cctgtcctgc tcttccaacc ccgctaagat ctctttttgt tgttactccg
    65341 acaacagaca tccccatgaa catcaaagta gctgtcaaga aagcttgcgt tactgttcca
    65401 atatcgtagt atgcaagagt aaccgctaat gttatgccat tcaaaagaga gtaggcaata
    65461 aatccaccga ttgctaagct cggattcttt tgtgctttta cgcctagaac cattactaaa
    65521 atcagttcaa cgatccacag acccacaaag cctagtggaa aattattgat aaaagttgct
    65581 aattgcaaag gaaataccca aagagccaag taagaagtga ctgcactaag ggcaagtccc
    65641 atacctaaaa atccataaac ttttgcataa aaacgattga tacctgacat tgatgtactt
    65701 ggattattca ttcaattcac cttcactttc gattgctatt tccaattcag gaacttgtgg
    65761 cggttggacc ataacgacag catccaacac acgatcttcg ttcgtattct ccgcttctac
    65821 agaaatcgtc accacatcct tcatcttatc tacttttatg acctcaaact gaaaagtcag
    65881 tgtttcataa tggaaaaccg gttcaataaa attgacagaa aaattgacaa catgtgatcc
    65941 tggtccagga agatgctttg aaattgcact tgtgatgatc cccatcagca tcactgatgg
    66001 aacgattggc tgttgatatt ctgttttttg tgcaaagtca tgttggatat acaaaggatt
    66061 tgcatcattc gtcaatccca aatacagcaa aagctggtta tcttcaatag attctgttaa
    66121 ggataacgag tcgccttcct cgatttcatt tattgttttt cctagttttc tcggcttacc
    66181 tatgttcaag atagaaaccc tccagtttat ttttaatcat acattattgt accaaaagta
    66241 gcgtaaaagg caagttctgc tcccttgatt taacagattc ctatagttta cagaggtcgt
    66301 gaaaaagctg tcctgcatca aataataaga acagccaaga aaaaacagct tctcatgttt
    66361 ttgatttgat tgggcttatt accgcagatg caagcttttg aacaccgttt atcataagtt
    66421 gcaaaaaagg gggcttggca agatttttgc cactccccct ctttctattt ttataaggac
    66481 actgcagtaa ttgtattaca gtacattttt cgtcgtgctg cttaaaagtt tttcatacgc
    66541 ttgttcaaat ttttgaacat ctccagcacc catgaagatt actactgcat catgataatc
    66601 taacaatgga gatacattat tttctttgat gacttctcca ccttttttga ttttcgctcc
    66661 aaggtcttca atcttaacgt ttccttgttc ttcccgagcc gaaccaaaaa tatcgcataa
    66721 gtaaacttta tcagctaaat caagtgcttc tgcaaattca tccatcaatg caatcgtacg
    66781 cgtaaacgta tggggttgga aaactgcaat tatttcttta tctggatatt tttggcgtgc
    66841 gccatcaatc gttgctttga tctctgcagg gtgatgcgca taatcatcga ctacggtcat
    66901 gtctgctacg atcttttcac tgaatcgacg ttttactcct gggaaagtca gcatttcttc
    66961 tgcaacttct ttcagatcca gtttttcgaa ataagctact gcaataacac ctaaagcatt
    67021 taagatattg tgtttaccga atgctggtac agtgaagtgc ccaacaaatt catccccatg
    67081 atagacatca aaagcagaac cagaagttgt gcgttcgatg tttcgtgctt ggatatcatc
    67141 gttttctgtg acaccataat aataaatcgg cacatttgct tttaatttgc gtaaataagc
    67201 atcgtcacca tacgcaaaaa tagctttttt cacttgtcca gccattgttt ggaaagctgt
    67261 aaatacatcg tcgatacttg tataataatc cggatgatca aaatcgatat tggtcatgat
    67321 tgcatagtct ggtgaatagg caaggaaatg acggcgatat tcacaggcct caaatgaaaa
    67381 gaattccgct tgcggatcac catgtcctgt tccatctcca attagataac ttgttggacg
    67441 gacaccactc aatacatgag agagcagtcc agtcgtactt gtttttccgt gagagcctgt
    67501 tacagcgata ctcgtataat tttggataaa atgaccaata aagtcgtgat agcgaatcac
    67561 ttccaaacct aattcttttg ctcgctggat ttcttcatgt gaatcaggaa atgcatttcc
    67621 tgcaatgatc gtcatccctg gttttacatt atctgcgtta aatggcagaa tcgtgatatt
    67681 tgctttttct aaatctcttt gtgtaaagaa atatttttca atgtctgatc cttggacatt
    67741 tagaccttgt tcatgtaaaa caagagccaa ggagctcatt cctgaacctt tgataccaac
    67801 aaaatgatac aatttatttt gattttccat ttgaatcctc ttctcttatc tagcgtccat
    67861 tcttttagct attacattca acacgctcct cattatctta taaacgattg aaaatttcaa
    67921 ccaaatttat ttggaaagga ggattcgctt gctaaaatgg tcaagtacga gaaaaatccg
    67981 tttttattga ctctttaatt ctggtgtaag aagaccaaac ttcctctgat atatcaatgt
    68041 gaggaataaa atcttttcct ttcggaccat agccgttctc atcgtcatgg agacgttcca
    68101 tttctcctaa tactagcgca tggaaatatt ccttaggcca ttgatgattc agttcttttg
    68161 ctgtaaagat gatctcttca gaaaagtgaa ggatttccgg cacaaatggc acagcattcg
    68221 cgaaaatagt ttgttcattt tgccatactt tctcaaaagt tgctcgagtc ctgcgttgca
    68281 gtgtcggatc attcatcaat aagatttttt ctggtacagc tcctaaggaa tgaagaatct
    68341 ccagtgaaaa agtggcgttc tctccagaat ttgtagactt cgattctatc agaaaagcct
    68401 tatcgggcaa atcatagact tcttttagat actgacgaca gatttcactt tcactcatgc
    68461 catcttcaaa atgaaatcct tgcttttcaa aattctcata aaggattcta gtcgcatgcc
    68521 ccacccctcc gaccaaaaac aattgatcta cttggccatt taaatacatt tgtgctgctt
    68581 tatcagctaa gataggaaga cagtttccag ctaaaataag tgtaggtgcc gtcagttcct
    68641 caaaatttcc ctcttcaggt gcggctaaaa aatttaatag ctgattccag tcttttagat
    68701 tcctctgctc acttttttcc aaaggccttc tatcctttca tctgattttt tgtgcttcat
    68761 taaagtcttc tctttttgtc tcaatccatt tgatttctcc atgacatctt ccacaacgat
    68821 atttgcgtac atcgattctt ctcttgcggc agatcgtggc agaacaatct tggcatcgat
    68881 aatactccat ctttatctgt ttcttcgtca aggctggtgc atagcgcaac cctcctgttt
    68941 ttttcaaaag atacttgaaa tccatatctt tatgctgata ccctcttcca gctagatgaa
    69001 gatgataatg gcaaagttca tgtttgatga ttcctgcaat cgtctcttcg tctgactctt
    69061 caaatagctt cgcattaaaa tctaaatgat gatcgtttaa atgatagcgc ccgccagttg
    69121 tacgtaagcg tggattgaac attgcttggt gcttgaaagg aagctggaag ctttttaatg
    69181 atatctcttc tactaactgc tgcagttctt cttgtgtcaa tttttgttac tcctcattgt
    69241 tactcttcat aaggggaaag catcgtcaaa ctgattctgc cttttttcgt atcgacttgt
    69301 tctatccaaa ctgttacgat atctcctact gacacgacat ctgtaggatg cttgacgaat
    69361 tttttgctga gtttcgatat atggacaagt ccgtcttgct tcacaccgat atcaacaaat
    69421 gcgccgaagt caatgacatt acggaccgtt ccagtcaatt ccatccctgg tttcaagtct
    69481 tccatactca atacatctgt acgtaataag ggcgctggca tctcatcacg catatctcgt
    69541 ccaggttgtg tcagcccagc taaaatatcg gctaacgttt cttctccgac tgataggctt
    69601 tcagccagtt tctctacgga taatcgtgtc aatttttcta ctgcttcttc tgtaccaagc
    69661 tctttttctg aaagttggac tgaagttaat atttcttttg caatagaata actttcggga
    69721 tggatacctg tgttgtccaa aatgtttttc ccaccaggga ctcgcaagaa tccgattgct
    69781 tgttcatatg cttttggtcc taagcgtggc acttttttca attgagttcg tgcggtgaat
    69841 tctccgtttt cttcacgata aatgacaatg ttttgggcag ttgttttatt caatcctgat
    69901 atatgctgca agagctgcgg gcttgctgta ttcacatcca cacctacttg gttaaccgct
    69961 gtttccacaa caaaatccaa ttgttcagcc aaacgttttt gagaaacgtc atgttggtac
    70021 tggcctactc cgacagcttt aggatcgatt ttgactaatt cagctaaagg atcttgcagg
    70081 cgacgggcaa tagaaacagc acttctttct tcaacttgta gatgcggaaa ttcttttcgt
    70141 gcaatttcgc tagcggagta aacagaagcg cctgcttcgt tgacgatagc gtaataggtt
    70201 ttatggtcgg cggatttcaa ctgctcagcc acaaaaagtt cagattcccg actggctgta
    70261 ccatttccga tagctaccat atctacttga tatttattaa ttaattggat aaaagctggt
    70321 ccagcagctt ctctttttgc ttgcgctgca ggtttatgag gataaataac ttcgattgct
    70381 aaaactttcc ctgtcgcatc tacgactgct agtttacatc cagtacgata agccggatca
    70441 aatcccagga caactttacc ttttagtggc ggttgcaaca aaagattacg gaggttttcg
    70501 ccgaaaatag cgattgcttg ctcatctgcc ttttcagtca attcattgcg cagttctcgc
    70561 tcgattgctg gctgaataaa gcgtttataa ctatcctgat aggcttctct gacgaatgaa
    70621 gaagaaggag aggcaggatt tttgactatt tggcgatcca agtaagctaa aattgctgct
    70681 tcatctactt gaaagaatac tttcagaata tcttcttttt ctccacgatt agtagctaat
    70741 attcgatgag aaaccatttt atggacgggt tctgcaaagt cataatacat ttcatatacg
    70801 cctttttcat ctgcttgctc atccttgaca ttgctgacat acataccttt gttatacgta
    70861 taagaccgaa tccaagtccg aaattttgca ttatcgctga cttgttcagc aataatctca
    70921 tgagcaccgt gcaacgcttc ttcggcagaa gagacttctt tttctttgtc aatatatttt
    70981 tctgcttcag attgcacctc tccatctgta agctgcatca gccacttcgc gagtggttca
    71041 agtccttttt ctttagcaat cgttgctttt gtccgtcgtt tttgtttgta tggacgatac
    71101 aaatcttcca cttgttgcat tttcacggct tgggtgattt ctgcttctaa ctcgggagtc
    71161 agtttccctt gttcatcgat tagccgaatc acttcagttt tacgtttttc taagttttca
    71221 agataggcat accgctcttc gatttcacga atctggactt catcaagact tccagtcatt
    71281 tcttttcggt aacgcgcgat aaaaggaaca gtgtttcctt catttaacaa attaagtaca
    71341 gtagtcaact gcttaggacg atagtctgaa agttcttttt gtacaagctg aatgatcgtc
    71401 tggttcaaat tttccgtcat taaaatgcct cttttctgct aaaaattgct tctttattct
    71461 atcatacttt ttatcctggg ttaaactact gaaagaaaca atattctcta taaaaataaa
    71521 aagagccgca acaaagtcac agctccttca ttctttccat tagtcagatt ccatgccaag
    71581 gtccttcgtt gttctgatat tcaatatctt gaagaatacc ttccttgtca aaaacaaccc
    71641 catgcaatga ccctccgaat acagccccac cgtccattcc gattttatga tcctgaatcc
    71701 agaggtctgt tgtctgcata tctccatgga gcattggtgt aatcgtatgt ccaaatacaa
    71761 ttgtcttacc tgtattgttt ttcccttcat gaaatggttc ccttatccaa ataaagtctc
    71821 ttagacttgt cttacgccag tctttctttg tcaaatccac accggcatgg acaaaaagat
    71881 aattttccca ttcataatac aaggggcgct ggactaaaaa tttaatcagt tctgaatatc
    71941 tcgattggat catcatcgag atttcagttg gggagtattc ctccgatgcc cctgggtgaa
    72001 tcaggctttc aaacgtttct tttccaccat ttctcatata aggcgcatac cagtcctccg
    72061 gtgattgcag aaattgcaaa aaatattctt catggtttcc ccggaggtaa atggctcctg
    72121 tttcttcgac taattgcttt cctaataaaa aacattcttt tgtacgagga cctcgatcat
    72181 tgagatcccc taataaaaca agctgatgga tctttggatc ataatagtga ataagttttt
    72241 caaaaagctc atgtttgcca tggacatccc caatagcata aatcaatgat ttcatgtgga
    72301 tcaccatcct caacgctagt atagcgcata ttggaaatgg tcgacaatca aaactattgt
    72361 tttctattta attaaagctg taaaccgcag ttctgcttct tctggagaga ttccatggtc
    72421 ttgaacatag cgattgcgcg gatgtttcgc acactctatg ctacatgctc ctaaatgttt
    72481 ttcttcattt tcaatggaag caagaatctg acgattgcac tcaggattgc tacaattgat
    72541 atatcgttca catggtgttc cgtcaaacca gtcgcgaccg acgatttgtt tatctacttg
    72601 gttgatatcg acagcgatcc gttcatcaaa cacatacatc ttgccgtccc ataattctcc
    72661 ttggacttca ggatccttac cataagtagc aataccgcca tgcagctgtc cgacatcttt
    72721 gaatccttca cggacaagcc aaccagaaaa cttctcgcaa cgaatgccac cagtacagta
    72781 ggtcactacc cgtttttcca taaattcttc tttgtgctcg cggatccagt ctggaagctc
    72841 tcgaaaactg cgtatatctg ggcgtacagc ccctcgaaaa tgtcccaagt catattcata
    72901 atcattccgt gcatcaatca ccactgtctg ttcatctaaa atagcttctt taaattcttg
    72961 cggtgaaaga taggcacctg tcacttggtt tggatcaatg tcctcttcta gctttaaact
    73021 tacaagctct ggacggtatc ttacaaacat cttcttaaag gcatgtcctt cgcttggatc
    73081 gatcttgaac atcgtatcag caaatcgagg atcagctttc attgctttca tataggcagt
    73141 cgtgttttca acggttccag acacagtacc atttattcct tcttcagcta ctagaatacg
    73201 acctttaaga tccaaagatt tgcaaaaggc taaatgattt ttggcaaatt cctctggttc
    73261 ttcgattttt gtataattgt aataaagcag tacttgataa tccatgtatt tcttccttcc
    73321 ttcttttcgt tcctaaacag tataaagaag gcgttcgcat ttgacgattt atttgcttgt
    73381 gtataattac gcatggaaag ttttttgttt acataatatt attatttgaa ttaaaaagac
    73441 ttcgctttcc tgtttcgcac agaaaaa
//
LOCUS       DACCCM010000006        64205 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCCM010000006 DACCCM010000000
VERSION     DACCCM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.006"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(147..1538)
                     /locus_tag="IVK78_000408"
     CDS             complement(147..1538)
                     /locus_tag="IVK78_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP6373905.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1751..2137)
                     /locus_tag="IVK78_000409"
     CDS             complement(1751..2137)
                     /locus_tag="IVK78_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP6373906.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2193..2816)
                     /locus_tag="IVK78_000410"
     CDS             complement(2193..2816)
                     /locus_tag="IVK78_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP6373907.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2880..3548)
                     /locus_tag="IVK78_000411"
     CDS             complement(2880..3548)
                     /locus_tag="IVK78_000411"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6373908.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3560..4000)
                     /locus_tag="IVK78_000412"
     CDS             complement(3560..4000)
                     /locus_tag="IVK78_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373909.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4059..4730)
                     /locus_tag="IVK78_000413"
                     /pseudo
     CDS             complement(4059..4730)
                     /locus_tag="IVK78_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4915..5313)
                     /locus_tag="IVK78_000414"
     CDS             complement(4915..5313)
                     /locus_tag="IVK78_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP6373910.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5310..6083)
                     /locus_tag="IVK78_000415"
     CDS             complement(5310..6083)
                     /locus_tag="IVK78_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6373911.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6080..6763)
                     /locus_tag="IVK78_000416"
     CDS             complement(6080..6763)
                     /locus_tag="IVK78_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6373912.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6930..7817)
                     /locus_tag="IVK78_000417"
     CDS             complement(6930..7817)
                     /locus_tag="IVK78_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6373913.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7814..8683)
                     /locus_tag="IVK78_000418"
     CDS             complement(7814..8683)
                     /locus_tag="IVK78_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6373914.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8820..10106)
                     /locus_tag="IVK78_000419"
     CDS             complement(8820..10106)
                     /locus_tag="IVK78_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6373915.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10379..10957
                     /locus_tag="IVK78_000420"
     CDS             10379..10957
                     /locus_tag="IVK78_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6373916.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10957..11886
                     /locus_tag="IVK78_000421"
     CDS             10957..11886
                     /locus_tag="IVK78_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6373917.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11883..13619
                     /locus_tag="IVK78_000422"
     CDS             11883..13619
                     /locus_tag="IVK78_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6373918.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13682..14518)
                     /gene="pheA"
                     /locus_tag="IVK78_000423"
     CDS             complement(13682..14518)
                     /gene="pheA"
                     /locus_tag="IVK78_000423"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP6373919.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14515..15024)
                     /locus_tag="IVK78_000424"
     CDS             complement(14515..15024)
                     /locus_tag="IVK78_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP6373920.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15017..16312)
                     /gene="aroA"
                     /locus_tag="IVK78_000425"
     CDS             complement(15017..16312)
                     /gene="aroA"
                     /locus_tag="IVK78_000425"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6373921.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRCIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16325..17416)
                     /locus_tag="IVK78_000426"
     CDS             complement(16325..17416)
                     /locus_tag="IVK78_000426"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP6373922.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17433..18599)
                     /gene="aroC"
                     /locus_tag="IVK78_000427"
     CDS             complement(17433..18599)
                     /gene="aroC"
                     /locus_tag="IVK78_000427"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP6373923.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18601..19665)
                     /gene="aroB"
                     /locus_tag="IVK78_000428"
     CDS             complement(18601..19665)
                     /gene="aroB"
                     /locus_tag="IVK78_000428"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP6373924.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19698..20720)
                     /gene="aroF"
                     /locus_tag="IVK78_000429"
     CDS             complement(19698..20720)
                     /gene="aroF"
                     /locus_tag="IVK78_000429"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP6373925.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21379..22353)
                     /locus_tag="IVK78_000430"
     CDS             complement(21379..22353)
                     /locus_tag="IVK78_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP6373926.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22334..24217)
                     /locus_tag="IVK78_000431"
     CDS             complement(22334..24217)
                     /locus_tag="IVK78_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP6373927.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24235..26460)
                     /locus_tag="IVK78_000432"
     CDS             complement(24235..26460)
                     /locus_tag="IVK78_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6373928.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26575..27609
                     /locus_tag="IVK78_000433"
     CDS             26575..27609
                     /locus_tag="IVK78_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6373929.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27676..28689)
                     /locus_tag="IVK78_000434"
     CDS             complement(27676..28689)
                     /locus_tag="IVK78_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6373930.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28767..28955)
                     /locus_tag="IVK78_000435"
     CDS             complement(28767..28955)
                     /locus_tag="IVK78_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP6373931.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29395..29523)
                     /locus_tag="IVK78_000436"
     CDS             complement(29395..29523)
                     /locus_tag="IVK78_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6373932.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29526..30899)
                     /gene="rlmD"
                     /locus_tag="IVK78_000437"
     CDS             complement(29526..30899)
                     /gene="rlmD"
                     /locus_tag="IVK78_000437"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6373933.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31010..32065)
                     /locus_tag="IVK78_000438"
     CDS             complement(31010..32065)
                     /locus_tag="IVK78_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6373934.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32075..33505)
                     /gene="gatB"
                     /locus_tag="IVK78_000439"
     CDS             complement(32075..33505)
                     /gene="gatB"
                     /locus_tag="IVK78_000439"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP6373935.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33502..34974)
                     /gene="gatA"
                     /locus_tag="IVK78_000440"
     CDS             complement(33502..34974)
                     /gene="gatA"
                     /locus_tag="IVK78_000440"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP6373936.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLAHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34974..35279)
                     /gene="gatC"
                     /locus_tag="IVK78_000441"
     CDS             complement(34974..35279)
                     /gene="gatC"
                     /locus_tag="IVK78_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP6373937.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35385..37421)
                     /gene="ligA"
                     /locus_tag="IVK78_000442"
     CDS             complement(35385..37421)
                     /gene="ligA"
                     /locus_tag="IVK78_000442"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP6373938.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37438..39672)
                     /gene="pcrA"
                     /locus_tag="IVK78_000443"
     CDS             complement(37438..39672)
                     /gene="pcrA"
                     /locus_tag="IVK78_000443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP6373939.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39827..41386
                     /locus_tag="IVK78_000444"
     CDS             39827..41386
                     /locus_tag="IVK78_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP6373940.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41497..43428)
                     /locus_tag="IVK78_000445"
     CDS             complement(41497..43428)
                     /locus_tag="IVK78_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6373941.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43446..44360)
                     /gene="pfkB"
                     /locus_tag="IVK78_000446"
     CDS             complement(43446..44360)
                     /gene="pfkB"
                     /locus_tag="IVK78_000446"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6373942.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44357..45109)
                     /locus_tag="IVK78_000447"
     CDS             complement(44357..45109)
                     /locus_tag="IVK78_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6373943.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45268..45477)
                     /locus_tag="IVK78_000448"
     CDS             complement(45268..45477)
                     /locus_tag="IVK78_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373944.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45488..46816)
                     /locus_tag="IVK78_000449"
     CDS             complement(45488..46816)
                     /locus_tag="IVK78_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373945.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47181..47768)
                     /locus_tag="IVK78_000450"
     CDS             complement(47181..47768)
                     /locus_tag="IVK78_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP6373946.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47851..49101)
                     /gene="clpX"
                     /locus_tag="IVK78_000451"
     CDS             complement(47851..49101)
                     /gene="clpX"
                     /locus_tag="IVK78_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP6373947.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49348..50637)
                     /locus_tag="IVK78_000452"
     CDS             complement(49348..50637)
                     /locus_tag="IVK78_000452"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP6373948.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50791..51723)
                     /locus_tag="IVK78_000453"
     CDS             complement(50791..51723)
                     /locus_tag="IVK78_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373949.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51880..52539)
                     /locus_tag="IVK78_000454"
     CDS             complement(51880..52539)
                     /locus_tag="IVK78_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6373950.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52542..53567)
                     /gene="sppA"
                     /locus_tag="IVK78_000455"
     CDS             complement(52542..53567)
                     /gene="sppA"
                     /locus_tag="IVK78_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6373951.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53580..53870)
                     /locus_tag="IVK78_000456"
     CDS             complement(53580..53870)
                     /locus_tag="IVK78_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373952.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54279..56084
                     /gene="glmS"
                     /locus_tag="IVK78_000457"
     CDS             54279..56084
                     /gene="glmS"
                     /locus_tag="IVK78_000457"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP6373953.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56156..57511)
                     /locus_tag="IVK78_000458"
     CDS             complement(56156..57511)
                     /locus_tag="IVK78_000458"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP6373954.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57535..58704)
                     /locus_tag="IVK78_000459"
     CDS             complement(57535..58704)
                     /locus_tag="IVK78_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6373955.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58701..59588)
                     /locus_tag="IVK78_000460"
     CDS             complement(58701..59588)
                     /locus_tag="IVK78_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP6373956.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59777..63469)
                     /gene="nifJ"
                     /locus_tag="IVK78_000461"
     CDS             complement(59777..63469)
                     /gene="nifJ"
                     /locus_tag="IVK78_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP6373957.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63668..64189
                     /locus_tag="IVK78_000462"
     CDS             63668..64189
                     /locus_tag="IVK78_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6373958.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17837 a        13925 c        11463 g        20980 t
ORIGIN      
        1 agctttttca caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga
       61 aatttcacaa aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga
      121 ccacttctat cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca
      181 ttgatgacat cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg
      241 ctaccgctga cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact
      301 aacccaacga ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt
      361 gcgcccccac cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc
      421 aaaataaatt ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac
      481 atagctggat aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag
      541 aaattagctg ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc
      601 aaagctccag cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg
      661 aaatagttga ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca
      721 gcaagcaatg ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa
      781 tcacttgttg ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa
      841 acaagcgtca cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat
      901 gctgcttctt ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg
      961 aaaataacga ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt
     1021 ttagggataa aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt
     1081 gttgccagtt cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg
     1141 agtccgaaca gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt
     1201 aaagaagtca atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg
     1261 aatgcaccaa tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca
     1321 tatccttgac ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca
     1381 ccaaagatca attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg
     1441 cgtgttgaaa attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca
     1501 aaaactagaa ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta
     1561 atgacaagtc catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg
     1621 aaaaaatttt tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt
     1681 ttgcttacaa ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg
     1741 tttttactgt ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta
     1801 aggcgatctc tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta
     1861 gctcgtccac ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat
     1921 gattgatctg actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta
     1981 agcgtgctcc tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg
     2041 agaagcctac tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa
     2101 atgcttctac aacttctttt tgtcttctga tttccatata attttatcac tcctttatga
     2161 aaattataac atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta
     2221 aggatttcat tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg
     2281 atgtcgtaaa gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga
     2341 tcgccgatca tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa
     2401 gatgctggat caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga
     2461 tgtgccaatc gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg
     2521 atgaaatgac gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat
     2581 ggacgggaaa cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg
     2641 gcattcaagt ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag
     2701 atttcttttt tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc
     2761 attgcgggat acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc
     2821 cttttgttcc attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt
     2881 taaagtattt ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt
     2941 gcatttggga tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc
     3001 agtgctcgga tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt
     3061 ggtgctagat gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct
     3121 ccgtatggga tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg
     3181 gttaacggcg gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat
     3241 atttctttca tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga
     3301 cgccatgttt ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg
     3361 gtaagaatcg cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca
     3421 agcatttttt gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct
     3481 agccatcctg tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact
     3541 aatttcattc ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg
     3601 tcacacgtat tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact
     3661 caaaaaaagt ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat
     3721 atttttcttt gactctatca agatatttca aataacgttg attctcattt agtctggggt
     3781 agattttttt tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt
     3841 gtccgattct atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata
     3901 gaccgcgatt caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct
     3961 gctgaaatcg tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta
     4021 atatttatta tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca
     4081 acttggtgaa ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct
     4141 tctctgttga ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc
     4201 tgttgcatac gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg
     4261 tgaagcagac ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta
     4321 ctgatcactt tttaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa
     4381 ttttcgtcat ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc
     4441 ttcccatcgt ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc
     4501 caagattcaa gagaaagctc gatataatct tttactggta attccaattc taccttaaaa
     4561 aagtgcaaaa cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct
     4621 agatggaact ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga
     4681 aatgtagatc gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc
     4741 cttttttcat catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga
     4801 atttcataaa aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg
     4861 aaataacgct catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga
     4921 ttcagacttt tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg
     4981 atattgatga atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc
     5041 attacctgga atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa
     5101 actatcgggt actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa
     5161 tttccctgaa tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc
     5221 tgcttctttt gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat
     5281 catgtgaaga ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt
     5341 tctttttcga ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg
     5401 gataatattg gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct
     5461 cttccttcaa tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc
     5521 gggcccgttg ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt
     5581 cgtcctaaaa taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg
     5641 acgagcaaac gccacataga caatcctact gctgcgccga tcccaattga aattaccatc
     5701 agcatgattt tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca
     5761 tgaatcgccg gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt
     5821 atataattgt aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa
     5881 gccatattga ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc
     5941 ataatgtcaa agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat
     6001 aggattacga taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct
     6061 atcacttgct gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg
     6121 gccctaacga agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga
     6181 tcatactagt catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag
     6241 tcactccccc cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga
     6301 tcaaaacgcc tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc
     6361 gaagcagcgc aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca
     6421 ctctgccaat ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag
     6481 gttcagcaat cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac
     6541 ttttcttttt gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg
     6601 ctccaaacaa aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag
     6661 cgaggtcttc tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca
     6721 tcggcaatat agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac
     6781 tctttaagtt taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt
     6841 aatttttctc aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga
     6901 ctttgaagga acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg
     6961 aattgctttt gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca
     7021 gccatgatca ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga
     7081 accgtccgtt tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta
     7141 ctcatgaatg tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact
     7201 ctaaaaaaaa tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt
     7261 ggaaaagatt taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc
     7321 aggatgacaa ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt
     7381 cgatataaag ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg
     7441 aaaattaggt cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa
     7501 atcagcaatg caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga
     7561 gtaaaattca gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc
     7621 tttatttttc ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca
     7681 aaagggaaaa tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca
     7741 attgtcgaac gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt
     7801 tctaccatat ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca
     7861 caataaagga aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt
     7921 tataactcaa attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc
     7981 cagtaatatt ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag
     8041 taaacaaaat gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag
     8101 aagcaccgtc aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa
     8161 ggaaaaaaat catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc
     8221 tcgcccaaat tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca
     8281 acaagttgtt tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga
     8341 ccatcgaagt gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact
     8401 ttttatcatt taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta
     8461 caagtaaata caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc
     8521 gctgataatt acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg
     8581 tgatcaaagc caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga
     8641 ttaaaaaagc atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc
     8701 tatcattgat tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc
     8761 ttttttgtct tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat
     8821 caattagcca attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct
     8881 tctccattga tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca
     8941 tgtccgtaat tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt
     9001 tgaccactgt aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc
     9061 gtagatacta gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac
     9121 gttgcttcta ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag
     9181 ccagcgccgc caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt
     9241 ggtgcaatct tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa
     9301 gcgccagttg ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag
     9361 tcagaggttt gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac
     9421 tttaaagatg cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct
     9481 cctgcctgtt gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt
     9541 ttctttcccg tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga
     9601 ttctccatat cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt
     9661 gctgttacgc ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca
     9721 aatttatacg gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct
     9781 ggataagaag tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat
     9841 ccttctgtat tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac
     9901 atcgtaacaa cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact
     9961 gctttgatat tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct
    10021 gtactactag cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact
    10081 ccagcaatta actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt
    10141 acgtttgcta cattcatcat accaaaattc gaaaacggtt tctttattat tcagatactt
    10201 caaaaatttc tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct
    10261 tagcatctag accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat
    10321 tttctcctag aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat
    10381 gccaatacgc gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt
    10441 gatcaattgg tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc
    10501 gcttgatttt tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga
    10561 attaaatgga atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct
    10621 tatcctctct agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc
    10681 tgattatctt ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt
    10741 ttcacaaaaa atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat
    10801 ctgcgagaat ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct
    10861 ttttgaacac accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa
    10921 ggctctaaac aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact
    10981 atttatcctg tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa
    11041 gatacactta ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt
    11101 gttgtttctg attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat
    11161 tgcaccaaag gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt
    11221 cttccgttat taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat
    11281 aaagactacg ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag
    11341 cctattgatc cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac
    11401 gctttggctg acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct
    11461 tctttattag ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt
    11521 gagaaactca cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg
    11581 atctacgagt tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa
    11641 aaatatcatt tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac
    11701 ttttcaaaat atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca
    11761 cggcttactt tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc
    11821 agagttttta aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta
    11881 aaatgagaaa aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca
    11941 tcggtctttt tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg
    12001 cctatatcca gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat
    12061 ttgaaagttt gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg
    12121 tcaaaagcta tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa
    12181 caatccaaaa aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat
    12241 tgatctttgg agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg
    12301 ccgattcttt ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac
    12361 aaatgctttt tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct
    12421 ttgtcaaaaa actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct
    12481 catccaagca actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct
    12541 ccttcatgaa cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc
    12601 gatcaatgat tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt
    12661 tgacccgtct tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt
    12721 ctttatctaa ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg
    12781 cttttgctgg ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct
    12841 tgattgattc gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag
    12901 gaagccatga agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc
    12961 atcattatga acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta
    13021 tgcaagcaca gatacagccc cattttattt ataatacgct gactagcttg aaatttttga
    13081 tttggcagaa aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa
    13141 gaagtacgat tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac
    13201 aaagttacat cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga
    13261 ttcctgatga actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa
    13321 acgcctatct tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa
    13381 caaaaaatca gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa
    13441 ataccaaaca aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga
    13501 tacgcctaat gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa
    13561 cgactgtaac gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa
    13621 gaatagccaa gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact
    13681 ttcatctacg cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc
    13741 ctccgagcat cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca
    13801 acaaatcgat cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct
    13861 tacttaagtt gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc
    13921 gattagcagg tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca
    13981 tctttcggtc accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct
    14041 cagctaagat ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt
    14101 cttctttcgg atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta
    14161 aaggaacatt aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat
    14221 gtgaaaggat ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa
    14281 gaacgatttc acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat
    14341 gaacagctcc ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa
    14401 acaaagcgcg caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata
    14461 gctgacaagc cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat
    14521 gcttttgatc ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt
    14581 gattcgatgt gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat
    14641 ttcttctctt gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg
    14701 atcaactaga cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa
    14761 acgccgattt tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt
    14821 tgacagcttt cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg
    14881 aaaatattcg gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata
    14941 ctctttttgt aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa
    15001 accaatcagc aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt
    15061 caaaaaatgc cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat
    15121 cagctatcaa agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca
    15181 cttttgcgcc atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta
    15241 tctttgctcc cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta
    15301 cttttaattc ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag
    15361 ctagaatggg caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct
    15421 tcaaatgact cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct
    15481 gggtaatcgc tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga
    15541 ccccgacatt ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga
    15601 aagcagctga tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc
    15661 cggtcactat gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt
    15721 cttccgtatg gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta
    15781 atgccgcaaa cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga
    15841 taggtgataa agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc
    15901 cttgcaagtc agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag
    15961 acaacgaagc atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca
    16021 ataaacgtat ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg
    16081 agtggctacc gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta
    16141 attgttggaa ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag
    16201 tcgtcgttcc ttcagcaatt gctccaaaca tgatgcagcg atgggaaatt gatttatctg
    16261 ctggtatcct aacagtccct cttaatccat gtatttgttg taataattgc atttatccag
    16321 cctcctattt gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta
    16381 acaaatcctg ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct
    16441 cttggatctt caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg
    16501 caataacgcc aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc
    16561 cttgcttatg gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga
    16621 tttccgattg gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat
    16681 ctgccaatcg ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag
    16741 gatctgagga agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc
    16801 gcggatactc ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg
    16861 gaaaatgact tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt
    16921 ttgctttggt tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca
    16981 aaggtgtaaa gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg
    17041 atttatgaga accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg
    17101 atgtcacgac ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt
    17161 tgattctttc aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga
    17221 taacgtctgc ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct
    17281 gttctactgc ttgctcacgg gtttttgact gactatccca accaactact atcagttctg
    17341 gattggtctg acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa
    17401 aaacatgttg tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac
    17461 tccttcactg cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct
    17521 gtcgctacca cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta
    17581 ctatccgaac gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga
    17641 agtggcttgt acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca
    17701 ttggtcattc cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct
    17761 tcttcccata ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg
    17821 accccaaatt ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca
    17881 tctagcttcg tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc
    17941 acttctacaa ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt
    18001 attctttgct caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg
    18061 atttcttgaa tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg
    18121 tgtcctgcta cgatgatgtc aagttcttcc agaatttttt tggcaattgc gccgattgct
    18181 acacgcatgg tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga
    18241 tggtatttca taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc
    18301 agtgattggt ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa
    18361 tctttatttt ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca
    18421 gacgtgatcc gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct
    18481 tgtctgcgag cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt
    18541 ccttcaataa tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc
    18601 ttacagtcct cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg
    18661 cccaatcttt tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt
    18721 tatcgcgtgg aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt
    18781 ttgaagtatc ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc
    18841 atgagaagtg atggctacca ttccaatagc tactccttct ccatgactga ctaccccata
    18901 tcctgctgta ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt
    18961 tccttggtca aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat
    19021 gattggaaca gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca
    19081 aagcgttgca tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg
    19141 acgtaccggc aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa
    19201 tgttccgaca aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga
    19261 atctacttgt gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt
    19321 cgaggccacg aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc
    19381 gctgcgggta aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga
    19441 tttactggtc tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa
    19501 ttggaacatg attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac
    19561 tttttgaggt ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc
    19621 aatatgaata tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt
    19681 cttttaaaaa gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc
    19741 gcaacttctt tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac
    19801 caagcatttg caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca
    19861 cccgcacgag ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca
    19921 atcacaggga aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta
    19981 cgtgtcgctg tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct
    20041 tcgactgcaa tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt
    20101 ttcaatccaa caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata
    20161 ttacgtgcgc caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca
    20221 tccatcactt cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc
    20281 aatccttctt cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca
    20341 cctcgcagaa tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct
    20401 aatccttcga tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact
    20461 ccgtctacat ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg
    20521 tttaaaatac gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa
    20581 cttcgcgtat caccttttag tccaatcact actcgttctg ttccttgatc gattgagact
    20641 ttgaatcctt ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct
    20701 tttttcataa ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc
    20761 tgattttctc tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct
    20821 gttgttctca ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga
    20881 tgtccctgta ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc
    20941 aaaaatataa aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa
    21001 aatctttagc catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca
    21061 aacctatcta aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat
    21121 aaaaaatcgc tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact
    21181 gtcaatcatt tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa
    21241 aataacaatt ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt
    21301 actctcttgt tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa
    21361 ataataatct aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct
    21421 gattcggtaa ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac
    21481 ttttgcaaaa atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc
    21541 ctgttccagt tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg
    21601 ttcaaatcgc aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct
    21661 tgttagttct acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg
    21721 ggaaacgggg agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg
    21781 atatgtttcc cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa
    21841 ccttaaaccc aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac
    21901 aagcatttta ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt
    21961 gttgatttcc agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg
    22021 attatttact ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc
    22081 tttgactttt ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct
    22141 acctctgtca ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca
    22201 ttcttttccg tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg
    22261 cgtattcccc gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt
    22321 atccatatga cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg
    22381 gtttttcttg tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt
    22441 tatcgtcaaa gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt
    22501 ggatcgcctg atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa
    22561 gcaacgtcat gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac
    22621 ataagatgaa cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg
    22681 catacatgca gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc
    22741 cttcataatg gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa
    22801 tgttttcttc cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt
    22861 ttaatgtttc gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg
    22921 cacctggtac gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt
    22981 cgaggttcgt ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga
    23041 tctggttggg gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca
    23101 aatctttttc catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc
    23161 gatttacccc gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt
    23221 cgattttacg aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta
    23281 tcgtctccaa ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt
    23341 caaatccatc gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt
    23401 ccttcccatc aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa
    23461 aagacaaatc tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc
    23521 aaagatcttc taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa
    23581 aaaatcggaa aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca
    23641 cagctagaat attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg
    23701 aatcttcggc atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac
    23761 cagcaaaatg aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca
    23821 aatacgaacc aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag
    23881 ggcttgtcgt gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga
    23941 tgtaatggat cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa
    24001 aagaacaggc atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg
    24061 aataacaaaa acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt
    24121 cttcccttgt tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa
    24181 cttgtggatc atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa
    24241 ctttttgaaa tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc
    24301 aactgtcatc aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc
    24361 ctcatcatct aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat
    24421 cctccctaaa aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat
    24481 attttcttca aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat
    24541 cgtttgatag gatataattt ggtcttttac ttgcttttct tcttcaggag acagctttgt
    24601 caaatccagt tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga
    24661 agtagatctg tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat
    24721 cggataagca aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc
    24781 acttgtccat gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc
    24841 agaacagcct tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata
    24901 aagcccgagc atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga
    24961 atagacatct gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc
    25021 taaaatcttt ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag
    25081 tacatgttgt gatctggaag cagaaggcat tctacctggc tcctgcataa gaaaatcagg
    25141 atgtgttcga tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa
    25201 tttcaatcct ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc
    25261 ttcatactcg aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc
    25321 atcatctaat acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat
    25381 tttttcacta ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc
    25441 agcaaattga tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact
    25501 cattttgttc aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg
    25561 gcctgcattc aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg
    25621 attgatctga tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc
    25681 gatcgcttct ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat
    25741 atggtgactg ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg
    25801 ccgtgaaatc tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg
    25861 cctagtatgg gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc
    25921 ggtttcattt cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata
    25981 gaacaaatcg aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc
    26041 ctctgacttg tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt
    26101 aatataatgg ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat
    26161 cgatgcgggc gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc
    26221 tttcgacaga gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg
    26281 gggataattt ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa
    26341 tacattgctt tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac
    26401 tcggctcttt tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat
    26461 catttcactc ccaacaaaac ggaaaccgtt tacttttttt tataattcct gtatactgtt
    26521 tactaaacaa ttttgtcaat aaaaattttt tatttttttg aggagtgtgt aaaaatggtt
    26581 ggaattagag atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg
    26641 aataacgatc agacgatcca tgtggctcag actactcggc aacggatagt agaggcagct
    26701 gctgcgttgg attatgacat cagtactcgt aaatatatca aaaagagaat gccttcgatt
    26761 ggtgtgatct ctacgatcgg caaagcgagc gaagaacagg atatctatta caaacatttg
    26821 cgagtaggga tggaagaaga agctcgacgt ctgcatttag ggatgaatcg aatctataat
    26881 ctatctgata atcctaaaca atgggaagat ctagatcagt taggtgcggt gatcgttgtt
    26941 gggactgtta cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc
    27001 gtggataacc ctgacatcca gcaagaggtc gatatggtgt atgcagatct tgaacggatg
    27061 actaaaaccg tactggaatt gttcttagaa aatggtcata aaagaatagc ctatatcggg
    27121 ggatataaag tcgatatgga tgaattcggt gggaagcaga tttctataaa tgaaaagagg
    27181 atgcgtgcct atcagcattt tatgatcgat catcacttag aaaaatatat gtcttatcat
    27241 ctaggtggct ggacagaaaa acaaggtgag gtacttgcga ctaatctatt agaaaatgaa
    27301 tcccctcttc ctactgccat tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg
    27361 gcattgaaac aaaaaaatat ccaaattgga gaagagattg ttgtagcaag ctttgataat
    27421 atcgagacaa tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca
    27481 attgggcaag cagctgttcg aatggcattg gagcgaattg acggtatacg ggacgaacca
    27541 atcattttga cttatcctgc acgattgatt gttagggaaa gttttatccc aagaacaaaa
    27601 aggtattgaa agagaggttg tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg
    27661 ctttttcaca acctcttact tatacttatt cacaaaatcc aatgtctgct ggtaacaatt
    27721 ttgcgattct tttagcgtgt aatcaaattg gtattcatgg gtaatctctt tttttgtatc
    27781 tgtataaaac aaggattgca cgggtacctt taattctgtc aaccgatttt ctaatgcttg
    27841 accttgctcc tggaatgagt atgcatttcc gtcagttata tacgttggcg gaaattcctt
    27901 agaaacatgg tcagccacac tagcttcttt caattgctca ctcgttttcc aatcttttgt
    27961 tccaatcaac gaccacgcaa cggttttgac gaaaaacttc ataaatcgat catccgaatg
    28021 ctgctgtgct gttagcttca aatcaacagg tccgcaataa gaaatggttc ctttcagact
    28081 atcttttgga agtagatgtt tgattttcat ttgtttagca tacgcatcat tcgtttgtat
    28141 agctgcatat tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa ataattgttc
    28201 catgtctagc aatttgtcgt ctttttcttc agctaataaa tctttgatca gttcattcac
    28261 ttgaagaact tgattagggt attctgaatc aggcgctaat tcataattca tcgcaaccac
    28321 tgcgatgttg gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg atttatctcc
    28381 gccgacgaat cctccaccat gtacccaaaa tagaacagga acaggcccct tgctgttttt
    28441 tggatagtag atgtcataag tattctcttt atacttggac tcatacgtca aatctgtttt
    28501 tttcgccacg tttttttctg cttgatcgaa cgttttttta tcagtgattt gaacagtatt
    28561 tgaaaacata tggttgatca agaatgctcc aggtctgggg gaaagctgga aggcaagaaa
    28621 gattgcaaac gcgatcccaa gaatgattcc gaagaaccat ccgataaatt taagaatttt
    28681 ctttttcata aggctccctc agtagttagt attaagcatt ctgattatac catagtgtta
    28741 tttcctatca agctctccta gatgatttaa tgattttccc caaacttctt ctgcaatgct
    28801 tttgacaaaa gaaatttttt ccattgttct tcaaacaaaa cattttcttc ttctgttgaa
    28861 gcaaatccac attgaggact taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg
    28921 tagatccggt caatcacttg ctggtgctct tccatttcgc catttttcgt tacagtcaaa
    28981 atctaagaca actaatttat cttcagagac tttctctaaa agaacaaaac tcccagcacg
    29041 ttcactatcg tattctaaat aataggcacg gacattttct tgagtaaaat aatgggtctg
    29101 ctacgtcgtc atcccccctg atgccgccaa aatcgaataa taatttccgc gtcaaacatg
    29161 tatatttagt gttgaatcat ttgagaatat gaaaataaaa atctttttga tcaatcagtt
    29221 aatcaaattg aaataacgaa aaatctgaga cagaatgacg ttcattctga cccagatttg
    29281 attttgcaca actcaacagt ccttattggc aaatcgccaa aataatgtag actaactaaa
    29341 aagactaaga aacaataaca aaagttaaca cttcttagtc tttctttagc acgtctattt
    29401 attaaagatt ttagggcctt ttctcatgtt tttacgatca ttcttaggtg tttccatttg
    29461 tttggatcgg cctccacctt gtttcttttt gatcaattct ttcatttttt ctttttggtc
    29521 catttttatt ctcctttaac gaatgaagcg actaattcga tatgatgggt ttgcgggaaa
    29581 agatctacag gctgaacttt cttcacggca taacctgctt ctgcaaataa cttagcatct
    29641 cttgcaaaag ttgccggatt acatgagata tagacgattc gttctggtga catttcgatt
    29701 gctgattcaa taaaatgatg atctaatcct tttcgtggag ggtcaactac gatcacagat
    29761 ggtttgatcc ctttttcttg ccattttttc ataacttttt ctgcttttcc gacttcataa
    29821 tgaacatttt cgattttatt atttaaagca ttgaactgtg catcttcaat tgcttctgga
    29881 ataacttcca tcccatacac cttatccact ctgtctgcta aagacaagcc aatcgtacct
    29941 attcctgaat aagcgtcgat cacaatgtct tttttatcta actcggcaaa ctcgatggct
    30001 gtttggtaca gatactctgc ttgttgagta tttacttgat aaaatgactt tgctgagatt
    30061 cgataggttt ttcctaatag ctgatcttcg atatagtctc tgccatataa aactttttct
    30121 ttgtctccta aaatcacatt tgttttttct tcattgatat tttggatgac cgaaaccact
    30181 tctggcaatt cttcatgaat agcttcagca atttccttgg atttaaagaa tttttcttta
    30241 cgagtaacta aaacgaccat catttcatgg gaataatgac cacgacggat aatgatgtga
    30301 cgaagctgtc cttcattttt ttcttcatta tatcctcgta cttggaaccg ttgcaagata
    30361 tctctgacta ctttgattgc ttgatcgatg acaggatctt ggatataaaa atcttcgata
    30421 gggactagat catggctatt ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg
    30481 cgaacaggga tctgtgcttt attacggtag ccgaatggtt ctgccattcc gaccgttgga
    30541 agtacaggaa cttctggcat cttagcaatc ttcgccatta catgctctac ttgtttttgt
    30601 ttgaagacaa gctgttgttc atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt
    30661 aatgcattgt tttctacagg ctgacgatca ggtgattgtg taataaatgt ttcgactttt
    30721 ccatatccaa acttattgcc tgcttttact actagtattt ctacttcttc tccagggagt
    30781 gcattttcga taaataatgg ataaccatcg atttttgcta cccccattcc ttcataactc
    30841 aagtcgacga tttcgactgt atatcgttcg tttttcttta ctatttgctg ttctgtcata
    30901 ttttgctcct ttaagcctat ttttcaatcc tactgaactc tactataaat cagctaatta
    30961 cacaactaaa accggcactg accttactac gttcagcacc ggcttttgat taattctttt
    31021 gattaggtgt ttcgtctgct atttttccgt caccgtcgat atcttcatcc gttaaacgtt
    31081 cgacttcttt tacgaattct ttgctgacaa cctctaattc ttcttcatct gtacctgtga
    31141 ttgcttcatc aggaatctga tcggtattgg caaagaactc aatgtgttga tgaagatttt
    31201 ggaaatgcat cggtgcttcc ccgccgtatt caccatctaa gttgatcatt actggtcgat
    31261 tgatatcatc ttctgtttca acgaacaaac tgcttgtttt ggtatagatt attcttggat
    31321 catcgacatg tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc tcaaagatat
    31381 ttgctgtctt cacaatgatt aaagaaaact tcccatcatc caatttggca tctggcgcga
    31441 tctgttcgaa tcctccaaca gagttcgtca gccctaggaa aaacatcgaa gcatttccga
    31501 tatattcccc ttcatcatac ttcaagcgca ttttgattgg ctttactcga gggagcatct
    31561 ctgctccttt cgcaagataa gcaagataac caaaaatact ctttagttct gatggaactt
    31621 cataagtcaa ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa ctgttatcag
    31681 aaaggccgat gtccattttt acggtttggt gtttttttat cacttctgct gctgctttaa
    31741 tattatctct aggaattttt aatgctcgag catagtcgtt cgttgtacca gctgggatga
    31801 tggccatttt aggacggtgt tcaagcggag cgattccatt aaccacttcg ttgattgtgc
    31861 catcgccacc agctgccact actagatcaa aacctagttc tgcgactcta catgcttcat
    31921 tcttggctga atctggttct ggtgtagttg cataggcgct ggcttcaaat cctgcttctt
    31981 ctaatgcctg taggatatcc gccaaatttt ttttcaataa ttcttttccc gaggtggggt
    32041 tataaattac gcgtgctttt ttcattttca gcctctatcg tttcgctaat tcatctttaa
    32101 gtagcttatt cacgacacca ggatttgctt gtcctttagt tgctttcatg atttgaccga
    32161 ctaggaagcc aaccgcacga tcttttccat ttttgaaatc atcgacagat tgttggttgt
    32221 tatccaaaat ttcattaatg attggcagta gtttgttcgg atcagataat tgcacccaac
    32281 catttttttc aactacttct tgggcttctc cgccatttgt gatcaattca cggaagactt
    32341 ttttggcgat ttttgagctg atcgttccat cggcgatcaa acgaatcatt cctgctaagt
    32401 tctcaggtgt caattttgtc tcagctaatt ccagtttctc actgttcaag taagcagaaa
    32461 cttcacccat caaccagtta gatgcttgct tggcatctgc accattttcc aaaacagctt
    32521 caaaaaaatc agacatttct tttgtaagtg tcaataccat tgcgtcatac tctggaagtc
    32581 caaactcacg gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa ctagctttta
    32641 ctttttgaat ccattcgtca tcgatcacga atttcgggat atcaggctct gggaagtaac
    32701 gataatcact agacccttct ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa
    32761 aacggcgtgt ttcttgttgg atttctcctc ctgaaagaag aacttttgct tgacgttttt
    32821 cttcaaaagc taaacctttt ttcacgaaag ataaagagtt caagtttttc aattctgctt
    32881 tcgtaccgaa ctcttcttgt ccataagggc gaagagaaat attggcatca cagcgcatag
    32941 agccttcttc cattttcaca tcgctgactt ctgtaaattg gataattgat cgcaatgctt
    33001 ctagataagc gtatgcttct tctggtgaac gcatatcagc ttcagaaacg atttcgatca
    33061 aaggcgtgcc ttgtcggttc aaatcaacgt aagaataacc gccaatacca tgcatgtttt
    33121 ttccagcatc ttcttctaaa tggacacgtt cgatccggat ttttttcttt ttaccttcta
    33181 cttcgatttc gatccatccg tcatgaccga ttggttgatc aaattgggaa atttgatatg
    33241 cttttggatt atctgggtaa aagtaatttt tccggtcaaa atgagtatct ttagagattt
    33301 cacagtttaa tgccaaagct gctttcatcc cgaattccag cgcagcttta ttcattactg
    33361 gcagcacgcc tggataaccc cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa
    33421 aatgagctgg cgctggagag aaaatttttg agttggtttt taactcgacg tggacttcta
    33481 gtccaatgac tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg gttgttgttt
    33541 atggaattct gttgcttgtt caaacgcata tgccgcttga tacatcgttt tttcatcaaa
    33601 ataattaccg atgatctgca atccaactgg taatccttct gataaacctg ccggtacaga
    33661 catacctgga agtccagcca agttgacagg gatcgttaag atgtcactca tgtacattgt
    33721 gattggatcg ttaatgttct caccgattcc gaatgcaact gttggtgaag atggcccgat
    33781 gatcaagtca taatctgcaa agactttttc aaaatctcgt ttgatcaatg tacggacttg
    33841 tcctgctttt ttgaagtaag cgtcataata acccgcgctt aaggagaatg tacctaacat
    33901 gatacggcgt tttacttctt caccgaatcc ttctgaacgt gagttgacat atacatcatc
    33961 taatgatttc acattttcag agcgataacc gtagcggata ccatcgaatc gttgcaaatt
    34021 ggaacttgct tctgatgagg caatgatata ataaacagcc acaccgtatt ttgaatgagc
    34081 caagctgact tcttccacaa cagcacctaa tgaacggaat gtttctgctg ctttcaatac
    34141 agcttctttt acgccagggg ccacaccttc gcctaagtat tctttaggta aagcaatctt
    34201 cattcctttg atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga
    34261 agtaccgtct ttttcgtcat aaccgctgat agctgtcaat gctaatgcat tatctttcac
    34321 cgtacgggtc attggtccga tttggtcaag agatgaagca aaggcaatca aaccaaaacg
    34381 tgagacacgg ccataagtag gtttcatccc tacgatgccg ttaaaagctg ctggttggcg
    34441 gatacttccc ccagtatcac tacctaaaga aactggaatt tgtccagctg caactgctgc
    34501 tgcagaacca ccagaagagc caccaggtac ttttgttggg tcccaagcat tttttgtttt
    34561 cttgaaataa gaagtttctg tacttccacc catagcaaat tcatccatgt ttagcttacc
    34621 aacagggatc atatctgctt gatagatttt atccatgacc gttgcatcat aaattggttc
    34681 aaaattgtaa aggatcttag atgcagcggt tgtcaagata tcttttgtta cgatattatc
    34741 cttgatacca attggaatac cagcaaggac gttgctttca tcaatccctt tttcatctaa
    34801 agcttttgcc tgttccaatg ctttttcttc gttcaatgta atgaatgcct cgacctcagg
    34861 ctctgtttct ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt
    34921 cgatacaagc atatgatgca gttcttctag agaacgattg tgtaattctg tcattatgcg
    34981 ccagcctccc cattatcgat gattgcgggt actttgataa agccgtcttc tgtttccggc
    35041 acatttttca ataattcatc gcggttccat ccagcttgcg cccgatcttg tcgcatcacg
    35101 tttactgttt caaccacatt cgacgtaaat ggaacacctt ccgtatctac ttcttccagc
    35161 atttcaacca tatcgatgat tttacctaat tggtcggtaa aacttgcaag ttcttcatct
    35221 gcgaacgcaa gttttgctaa tttggcaacg tgtttcactt gttcttcact aatcgccata
    35281 ctaccccttc tttctaaacg agttaaaaac tttgctctct attttaacac aagagtgccg
    35341 tgtctgaaat tgtttattga aatttgtctg aaaaaactca tttttcattc ttctactgag
    35401 cgacttttag ccaatgcatc tgccatttga tcttctgtcc aaacttcgat tcctaattct
    35461 tgtgctttgg tcagcttgct gccagcatct tctcctgcta ccacgatatc cgtttttttc
    35521 gatacgctac cagtcacttt cccgccaaga ttttcgatcg tctcttttgc ttcttcacga
    35581 gtaaatcgtg ttagtttacc agtaaggact actgtttttt ctttaaaagg agactcgact
    35641 tcttgcaatt gggcatttcg aagccctttg tattcgaaat tgacgcctgc ttgttttaat
    35701 tcattcatca attcatggac ttcttcgtta ctgaaataag tgactacact gtcggcaatg
    35761 atatcaccga tcgtatcaag cgcaataatc gactcatagt cacttttact caatgtttct
    35821 agatcaccga aatgttctgc taaaattttt gctgctttcg ctccgacatg tcggattcct
    35881 aacccgaaaa tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata
    35941 ttatttgctg atttttcttt tattttgtcc aacgtcaata actcttcttc tgtcaatttg
    36001 taaagatcag caacatctgc tactagtttt ttatcataca tctgttctag tacgcgcggc
    36061 cccaacccat cgatattcat cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt
    36121 tgagcagggc atttaggatt gatgcaccgt aaggctactt cttcatccaa atggacaagt
    36181 tcacttccac aaactggaca atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt
    36241 ttatctaaca cgacttgcga aacttctggg atgatgtctc ccgctttata aatcagaact
    36301 gtatctttca agcggatatc cttcaactta atataatcac cgttatgcag gctggcacgg
    36361 cttactgtcg ttcctgctac tcggactggt gtcatgatag ctgttggcgt tactacccct
    36421 gttcgaccga ctgtccattc gatgttttcg attagcgttt caacttcttc tggcggaaac
    36481 ttgtaagctg ctgcccatct cggtgctttg atagtaaaac caagttgatc ttgtagcgag
    36541 aattcattca ctttgatgac gattccatcg atttcatagg gcaaatccac acgtttgtca
    36601 tgatattctt caatgtatga ccacacttca tcaatcgaat gacatagacg tttttcccga
    36661 ttcgtgtgga atccaatttt ttctagctct tctagcgcat catactgggt ttttgcttgc
    36721 ataggaccga aatcagctac tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt
    36781 ttagaatcta actgacgcaa agaaccagct gcggcgttcc gaggattcgc aaagatatct
    36841 tttccctctg cttctctgtc ttggttcaat tggacaaatg accgttttgg catgaaacat
    36901 tcgcctctta cttcgatgtt cattggttct tttaatttga ttgggaccga ccggacagtt
    36961 tttaggtttt cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc acctctcacg
    37021 aaatttccat cttcataacg caaagaaacg gaaagtccat ctatttttag ttcacagcaa
    37081 tagtcaacta cacgtcccac tgctttttgt acacgttgat caaaagctat cagctcttct
    37141 ttgctgaaaa cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt ttcaaaacct
    37201 tctaatactt ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa atcaggatat
    37261 tgttcctcga ttgctactaa ttctgcgtat tctttatcat aaacatagtc ttccactgtt
    37321 ggtttatctt ctacgtagta ttcgcgagac cattggttca gtcttgtacg aagttcttct
    37381 gctcgtttag cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc tccactacta
    37441 agctttttca attggcgcaa aggcagccaa aagacgtttt actccttgac ttgggaaagc
    37501 cacatctagt tctagatcct tcgacgtccc gcttactcgg acgactgttc ctagacccca
    37561 ttttttgtgt ttgactttat ctccaggttt ccagctgtta ttttcccctc cagtagcact
    37621 tttagaggga acaaccgctg tctctggctg tttatagcta tatgctgttt ttttagcgaa
    37681 tacaggcgtt tttttaggcg taggacgcat gccttcgatt tccagtaact cctgatcgat
    37741 ttcttctaca aaacgagaag gtcggttata ttgcgtccgt ccatataatg ttctggagaa
    37801 agcatttgtt aaatacaacg cttcttctgc tcgcgtgatc cccacatatg cgagacggcg
    37861 ttcttcctcc agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc
    37921 tagaccaatc aggaatacaa ctggaaattc taatcctttg gctgcatgca aagtcatcaa
    37981 tgtcacttgg gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt
    38041 taagaagacc gttagttttt cttctggtgc atcagcgtct tcttcatttt gctgttcgaa
    38101 ttgtttgtcg aattcttgtg tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg
    38161 tgcttctaat gtattttgga tttttaagtc ttctagatat ccgctacgat ccaaaacctc
    38221 tttcgtcaat tctgtcactg tcaagtatgg gatcatctga gtgacttctt ggatcatctc
    38281 tccaaatgca cctaattgct gtcctgcttt tccaccgata tttgccagat ccacattttg
    38341 cgctgcttca agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt tttcgataga
    38401 tcctggtccg atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg
    38461 aggatttacc agcacattca aatacgctaa aatatctttg atttctttac gatcgtagaa
    38521 cttgtgccct ccaaccattt tataaggaat atttgctttc aacagagttt cttccattac
    38581 acgagattgt gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct
    38641 tcgaatctct tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc
    38701 acggtagtaa gtgatcttgt ttccttcttt gttttctgtc cacaagtttt ttggttttcg
    38761 attgctattg ttttcaatga cttggtttgc tgcgctaagg atgtttttcg tggagcgata
    38821 gttttgttct agcaaaatta ctgcagcatc tggataatct ttttcaaaat ccaagatgtt
    38881 ctgcatatct gcaccgcgcc agccgtaaat actttgatct gcatctccca cgacgcacag
    38941 gttcctaaat ctacctgcca ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg
    39001 gtactcatct acatgaatat aatggaattt gttttgataa tacgtgagtg aatcaggatg
    39061 ttcttcaaat aatcgaatcg tattcatgat caggtcgtca aagtccatac attgattata
    39121 ttgaagctct ttttggtaag cagcataaca ttttgccgca atctcttcgt aataactgcc
    39181 ttgcattttt gcaaaatcct gaggggtctg caggctgttt tttgcttgag agattgttcc
    39241 taatatactt cgaggatcat attttttcgg atcaatgttc agttctttta gtatccgttt
    39301 catcaaggtc agctgttcag aagaatcgat gattgtaaag ttccggttat acccgataaa
    39361 atcaacatct cttcttaaaa tacgcacaca catcgagtga aaagtcgata cccagacatc
    39421 ttcgcctcca gatgcgagaa tcgcattgac acgttctttc atttcttttg cagccttatt
    39481 ggtaaaagta atcgctagga tgttccaagg attgacttct ttttcctcaa tcaaataggc
    39541 aatgcggtgt gtcagcactc tcgtctttcc acttcctgca cctgccatta gcagtaatgg
    39601 tccgtcagtg tgaagaacag cttctttctg acgggggttc atcccgttta ataattcagc
    39661 tttggatgac attaacaaca tcctttcttt tatcaatctt gttaattata gcattttctt
    39721 ggctttcatt aaactagcta gttcaaaatc atttgtttct ttttatggat ttttccttgt
    39781 caattcgttg atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg
    39841 aaaaatcaag aaattagata agactcagat atattttatt ctaaaaggaa tatttgttgg
    39901 actgctgaca ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc taagcgattt
    39961 agttcgaagt atctatgaaa tgagtcggga acagccgatt tacttaattg gcctagctat
    40021 ttgctgcatc cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa
    40081 gggaagcggg attccacaag tagtgggaca gcttcgcggg gaattatcaa tgaactggtt
    40141 ctctgtttta tggaaaaagt tcattggcgg tgtactttca gttggtgcgg gcctttttct
    40201 aggtagagaa ggaccttcta tacagcttgg cgcatccatt ggacaaggag ctggacaatt
    40261 gtttcggagt ccatcttcag aagaaaagat tttaatttct agcggtgcaa gtagcggact
    40321 tgcagcagca ttcaatgctc ctatcgcagg attactcttt gttttagaag aaatccatca
    40381 ctccttttct ccacttgttt ggctgacatc atttgcttca gcaatcactg ctaacttcgt
    40441 ctctctttac tttttcggct tgagacctgt tttatatcta ggtgaccttc cttctttgcc
    40501 gttgaaatat tatggttcct tagtgatctt aggagtactt ctaggcatct taggttttat
    40561 ttatcagaat gtcttgctgg ctttgccaaa attttacaac cgattgcctt taccagctta
    40621 ttttcacggg ttcgttcctt tcttgctgat tttgccaatt ggttatttat ggcctagtac
    40681 tctaggcgga ggcaatcaaa tcgttttagc ttttggacat gcttccttgc caatcattat
    40741 gattatcggt ttgtttgttc ttcgattcgt tttttccatg atttcttacg gtgggggtct
    40801 tccagggggg attttccttc cgatattgac attgggggct ttaatcggta gcttttatgg
    40861 aaacattatg gttgatttcg ggatggatcc aatctacatc aaggattttg tcgttattgc
    40921 catggctggt tatttcacag cgattggaaa agcaccgctg acagcaatta ttttagtgac
    40981 agaaatggtt ggctctctta atcatctgat gcctttagga ttggctgctc ttgttgctta
    41041 tatcgtcaat gaccttctcg gcggtaatcc aatttacgaa tcattattgg aacgtctact
    41101 tggaaatact cagaccgcct cactaaaagg tgcaaggaca acttttgatt ttccagtaac
    41161 ggcagagagt tctttagacg gattaatggt acgagacttc aattggccta aaaatatgct
    41221 gctaatctct atacgcagag gaaatcagga aatcttgact cacggtgata cgatcatgaa
    41281 tgtaggagat attttggtta tcttgactga tgaagcaaac ttgccaagaa tcaagcagga
    41341 aatcgaggct aaatcttcta tcacaaattt gaagcaatca ttttaacgaa catggaaaaa
    41401 ctattttaca acaactattc atctacacta cttgattcta tatgattcta taaaaaaaga
    41461 agaggctggg aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc
    41521 ttttctcaat aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa
    41581 atacatgacc gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa
    41641 tttgattccg aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg
    41701 aatggcacga atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag
    41761 tcccatcacg atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa
    41821 caaacgtgta gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc
    41881 catcacgaca cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata
    41941 agcggctttg ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc
    42001 aagtaaagca gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat
    42061 tgctttcata gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac
    42121 tggataaaag aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt
    42181 acgtaaaaat acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc
    42241 tgcaccgcct gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc
    42301 accgatactt gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg
    42361 tccgatttgg ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc
    42421 atgtggcacg ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc
    42481 aataacgaaa ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc
    42541 gatagtacca tccgcatttc cttcttctga actgctttct tttccatcac catggaatac
    42601 tggtgcagta ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact
    42661 gacaggacga ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc
    42721 tgcaacgatt acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc
    42781 ttctgaaccg ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa
    42841 agcatcttct gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac
    42901 gaattttctt tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc
    42961 ttctgcttct ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt
    43021 tgcttctttt aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa
    43081 tgcttgcaaa tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt
    43141 tggctgtccg tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt
    43201 tgcttcttta actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt
    43261 agaagtttgt gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg
    43321 gccggcatca aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg
    43381 caaatccatg atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct
    43441 tgaaactaat caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga
    43501 gctgccaagt catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct
    43561 tcgattggat ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct
    43621 gcacctactg aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc
    43681 gtgaagagca aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct
    43741 agtaattttt tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc
    43801 tcagctaatt cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt
    43861 aggtcatcac ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc
    43921 agttcttgat aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata
    43981 tctccttgtc ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata
    44041 gatgggccta agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt
    44101 gtatcttctt ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa
    44161 ataaattcgc cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt
    44221 ttcaaaatac gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt
    44281 cggttcaaat cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta
    44341 agtgttactg tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat
    44401 taacgaattc taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag
    44461 aggtgaagga caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat
    44521 ttttcatggc taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt
    44581 caagatgggc accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt
    44641 ctatcgcatt agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa
    44701 tggtagcaag ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg
    44761 tgatctgttt tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat
    44821 aaatgacatc ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt
    44881 gaacgttttt gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct
    44941 tagctccgcc atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc
    45001 ttatcgttga ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct
    45061 gctgttcaag caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca
    45121 cctcaataca aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt
    45181 tcataaaaat tataaatcag tcaaaaaaaa ataaaaaacc agtcagaaag actggttttt
    45241 tatttttttg tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt
    45301 catataaaca gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc
    45361 gccaaacatg accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag
    45421 acagacggat aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt
    45481 cttcacctca tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt
    45541 gcacacgcag atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct
    45601 ttattcgttt gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg
    45661 acagggattc tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat
    45721 ctgttacctc tacttctaga tattccctaa tataataaca ataagcttcg attccattat
    45781 ccaaagcaac agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa
    45841 cccaaggaaa atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc
    45901 cttgttggat atcataactt tttgttacaa aaaatgaagc attcttttta ggaacaggtt
    45961 gcatttgtgc attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa
    46021 cgaatggcgg tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct
    46081 ggtccctaaa ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc
    46141 gataaagctg gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga
    46201 tgtcgagaaa aatcggttta gggatgattt gatgataata gcgactataa tacatttccc
    46261 aacacgaagt gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat
    46321 cgactactac ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt
    46381 tctcaaacaa tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag
    46441 ctcgcatata ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca
    46501 aaaattgctt ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat
    46561 cttccaagcg aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc
    46621 ttaccgatgc tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt
    46681 gtgtgaaaga gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc
    46741 ttatttcctg ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt
    46801 tatcttcgcc aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa
    46861 tgataaagaa aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga
    46921 ctaagtaaaa agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa
    46981 tttagataaa cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga
    47041 agagatcgat cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca
    47101 aaagtaagtt aagctcaaaa aaagacttac cagccaaact tttatgaaag agtttgactg
    47161 gtaagtcttt tttattcttt ttatgattca attgcttgtt cgattgctgc ccacgcttct
    47221 tcttttccct gttttgtaac ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc
    47281 aacgcttttt tgatttcgct ctcatgcttg ttccatttac cacgagggat cttatctgcc
    47341 ttagtggcaa caacaattac agggatatca taatacttca aaaattgata catctggata
    47401 tcttcttgag aaggcttatg acgcaaatct actaaagaaa cgaccgcacg tagctggtct
    47461 ctagtcgtta aatatgtctc gatcatcttt ccccattttg ctcgctcagt tttggatact
    47521 tttgcatacc catatcccgg aacatcgaca aaatgtaatg cgttctcgat caaataaaaa
    47581 ttcaatgtct gggtctttcc tggcttaccg gaagtacggg ctaaattctt tcgatcaatc
    47641 aatgtgttga taaaagaaga tttccctaca tttgatcgtc ctgccagagc tatttctggc
    47701 aattgtgtat ctggatattg ttttggtgcc actgcactta tgataatttc agcttgatgg
    47761 actttcatgg tctctctcct taacaaggtg atgaataaag caagcggctg tcgcaaaagc
    47821 caaaatgact tttacggcag cctcttttca tcaacccgct tttttctctg aatctttata
    47881 gattactgtt ggctcaccag acaattcagc tgcttctttt gtgataatca ctttttcgat
    47941 gttttcattt gaagggatat caaacatcac atccatcatg atttcttcaa tgatggaacg
    48001 aagtcctcga gcaccggtat tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc
    48061 aggttcgaat tcaagttcgg tattatctag agaaagaagt ttttgatatt gtttgactag
    48121 cgcatttttc ggctcagtca agatccgtac cagatcatct gttgtcaatt tctctaaagc
    48181 tgccataaca ggtaatcgtc cgataaactc agggatcaat ccgaatttta gtaaatcttc
    48241 aggaatgata tgctgcatga cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa
    48301 tccaatcgtc ttttcgccca tacgattctt cacaatcgtt tcgatgccat caaatgcacc
    48361 acctacgatg aacaatatgt tcgtcgtatc aatctgaata aattcttgat gtggatgttt
    48421 acgtccgccc tgaggtggca cacttgcaac agttccttct agaattttca gcaaggcttg
    48481 ttggacacct tcgccggaaa cgtcacgagt aatagataca ttttcgcttt tacgcgcaat
    48541 tttatcgatc tcatcaatat agatgatccc tttttctgca agttccacat tgtaatcagc
    48601 agattgaagc aatttaagca aaatgttttc tacatcttcc cccacataac cagcttctgt
    48661 caaacttgtc gcatctgcga ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc
    48721 taaaaatgtt ttaccagacc ctgtaggtcc gatcaagcag atattgcttt tttgcagttc
    48781 aacatcatct gtgttttctt gtgcttgctg attgacacgt ttatagtgat tgtatacggc
    48841 aacagctaac gagcgttttg cgcgatcttg tccaatcacg tagttattca atacttcaag
    48901 aatttctagt ggtttaggga cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc
    48961 atcaatgatt tctttacata ggtcgataca ttcattacag atgtagaccc ctggaccagc
    49021 tacgatcttt ttcacttctt cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt
    49081 gctacttgga ttgtcataca tggtcttcac cccttaactt gcaaaaagta tgcaagaaac
    49141 tatcaaagac tttacagtct tttactatca taacatagtt tagtaaaaag aaaaagtgtt
    49201 taacaaagga tcaactgacc tatacctttg cattttttac tcttaacaaa aaataaggaa
    49261 ctgtgacaaa atcgtgtcac agtcccttat ttcgaataaa caattttcta gaagtagctt
    49321 cttcagaaat gatttattac ttgatttcta ttctttcgct tctgtcgctg tacctgtgat
    49381 caattcgatt gcttttttca ttgtcacatc atgttccagc atatcttctg tcaacacgcg
    49441 tttgatttgt tcgattggca tgttgtatgt ttctgataat tcttggattt ctgcattgat
    49501 ttcttcttct gttgcttgaa gattttcagc agctgcaatt gcttcgatca caagatttgt
    49561 tcttgtgcgc atttctgctt caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc
    49621 agttaattga tagtacattt caggagagat accttgacgt tgcatgttgt tcaagaattc
    49681 gtccattgaa cgatgtactt catcatgaac catcacgtgt ggcaattcaa cgatttctgc
    49741 gttttcaaca gccatacgga tcgctgcttc atcttttgca tcgtccgctg ctttttcttt
    49801 tgtttctact aattctttgc gatatttttc tttcaattcg tctaatgttg atacttcgtc
    49861 atcaacatct tttgcgaatt cgtcatctaa ttcaggcact tcttttgctt ttacttcatg
    49921 gattgtcact ttgaagactg cttcttttcc agcaagatct tctgcttgat aatcttctgg
    49981 gaaagttaca tttacatcta gactttcacc tgatttatga ccaactaatt gatcttcaaa
    50041 acctggaatg aatgagcctg aacctaattc aagtgagtaa ttttcgcctt ttccgccatc
    50101 aaacgcttcg ccatctacaa agccttcgaa gtcgattact actgtatcgc cattttcagc
    50161 tgcagcatct tctttgataa ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc
    50221 atccacgtca gcatctgtta cttcacggtc ttgtttttca acaactagat ctttatattg
    50281 tcctaatttt acttctggtt tcactgttac ttcagcagtg attacccagt cttgaccttt
    50341 ttccatgctt tcaacatcga tttttggttg agaaacagga tcgatgccag cttctttgac
    50401 agcagcttca taagcttctg gtaatacagc attcaatgca tcttcatata aagcttcttc
    50461 accgtacata cggttgaata cttgacgaga aactttccct ttacggaatc caggtacgtt
    50521 caagcttttt ttcactttat taaacgcagt tgttaatcct ttttggattt cgtcttgcgc
    50581 aatcgtaaat tttaaaacac catcattcgt gccttttttt tcaaatgttg cagtcattta
    50641 attccctccg agtttagaaa ttcattttat acatcaaaaa cgtttcatgc atacctttaa
    50701 atagtacact aaccctttca aattgtaaac atattgaatg gatagaatcc ttgtttttca
    50761 taacattttt atctttttca ctgataaaat ctaatggaag aagatcattt gctgcaaaat
    50821 tttttctctt ttttttaaaa aatcttgttt ttcatcttct gtaccttcat acatcgtttt
    50881 tcctgtacac gcctctacag tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc
    50941 atagacagga taaacaattg cagattgcaa cgtgaattct tcttttaaca cggaactaag
    51001 cagcgcatct tcattcccgt aacgatctga aaatacacat aattgctggt aacttgcctg
    51061 ctgctgcggc tttggcagat cttttggtat gactttgact aacttttctt caatcgtcaa
    51121 atagaaaaca ggatggcatt cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc
    51181 taaaatatgg acctctggcg atacaataag tttactcgct gtttggataa actcatgata
    51241 aggaagctgt tcgatctcat caatagacgc aagctgacgt gcaggttcca acgaaggaag
    51301 ttcctccgat ttattgtgga gtgccttgat cttctgcctc tccatttgtc cggataattc
    51361 ttctgcaaat tggatttttt caatgacccg ctgctcgaaa gcttcataaa aatctgtact
    51421 tgctatcaat tttctagcat ataaaaaatc ttgtgtactg attaacaagt caaaataaaa
    51481 ttcagcaaac tcctcatttt gaagatactg tttttcatgc aagagtgctt gcttcaatgc
    51541 ttcttttttc tcgtccaact cgtaaagaca aaaaacaagc aggctattag cctcaaagct
    51601 ttctttcaac tcgtaagctc gttgaaataa ttcttttgcc tgctgatatt gctggtttgc
    51661 tagtgcttgt tgtgccactc gcattaaatt ctcatatttt tctggaaaat caatcggctt
    51721 cacgcagtgt cctcctcttg ctcatattca gctcatgttc aatagtagca aaacaaaaaa
    51781 agcatgacaa ttaaaaataa ctgaagcacc ctctaaaagc tagattttag atctgacttt
    51841 tagaaggtgc tttcaactgt tactgctttt tattgctcat cagctgttga cttcctggtt
    51901 gaacgctttg atcagattga tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg
    51961 ttgctttttc tgattaaaga ctgctggaag ataccctaac atcagcaaag gaaattttag
    52021 aatgaaccgg caagcacctt ctctaactaa aacggttgac cagctaagtt cttcttcatc
    52081 aaagctgatg actcgaatac caaagatcat tttcccaatc gtttggccat gattgaattt
    52141 cgtcaataaa ataaaatacg ctagataaat aagtaaggct gtgaatccgt agagactcag
    52201 ataagaatct gaaaaattaa gccaacctag atttttggca ataccaagtg ttaaacgcgt
    52261 aatagctccg atacacaaaa gatcgaccaa gaaggcaaac attcggataa aaaaacctgc
    52321 atagaaatac ttaggaaaat catttgcttt tggcggttca tttcttgtgt ccgtatactt
    52381 tttccattta cgttgtttct ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga
    52441 gctcagtacc ttttgggaaa agttggagtc aggtcctaca gcaggctgct gttgcttatc
    52501 tttttcttgt tccttatttt catttgaaag ctctgacatg cttactcacc tccgtaataa
    52561 tacattgctt ttggtgattc cgctgtccct agattctcaa gaacggataa gatctgacta
    52621 gtctcagaag gcttcaatcc ctggaattct gctagtttac ttcctaacca tgtactagca
    52681 aacccagatg gcgtggaaga atattctact agttccgcat cttcaagctg ttgttccttt
    52741 cgtatagctt ttaaagcatc ttcaggaaat cccaattcat cgactaaacc tacttctttt
    52801 gcttggacac catcataaat ccttccatca gcaatttttt tgacttcttc ttcagattta
    52861 ttccgtccct cgctgacgat tgctacaaaa cgactatatg cgctgtcgat atatgcttga
    52921 agcaccgctt cttcttcttt tgtctctggt cgcgttcctg aacccatatc tttcaacgct
    52981 ccgcttttaa ctgttgtatc ttccacaccc agtttttcta gaagtcctga ataattcaat
    53041 cctgacatga tcacaccaat tgagccggtc actgtttctt ctgttgcaaa gattttatct
    53101 gcttgagcag aaatatagta gccaccgcta gctgccatgt ttttcatgct gacatacatt
    53161 gggatatcat gttctttacg aatcgtatcc aatttcttag cgatttccgc actttcgtag
    53221 acaccgccac caggagaatt tacttctaaa aagatacctt tgactgttgg atcttcttca
    53281 atcgccttca gttgttccat aaaattttta tgattgtaac cttcagaagc aaataaacct
    53341 gaagaccctc ccgcagcaat cgttccatct actgataatt ttacgatttt ttgcgtactg
    53401 tctccctctt ccaacacatt tggagctagt tcttcatccc catataacca actgttcaat
    53461 ccctcaattt gatcgttttc ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca
    53521 aataatcctg cagcaatcag aactgctgcc caacgtcttt tattcattct ttctcctttc
    53581 taatacaagt tttttatgac gatcacagtt tctgactgct cagaagtgtc gaaccgctct
    53641 gctttttctt cccgcgcata cccggaaaac gactcttttg ttaattcatc ttctaccgta
    53701 aactcacgag cttgagcaac ttccaaggca tcaggtgcga tttcttggat gctatcccag
    53761 ccagtatcta aaaatatttt cgtatcatct gatacaccag ctgatgcttc gaatttggac
    53821 aattgttttt ttaacattcc cagtgtcatg atctcaattt ttgaatccat ttttactcct
    53881 cctctatccg attactttct attgtaccga actcctgaaa aagatgatag ataaaaaccc
    53941 attatttttt gacataaaaa ggacatttga caaagaattt gatatatacc aatttatttt
    54001 ttattctttt tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga
    54061 taaaaaacaa aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag
    54121 atgacgagga gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca
    54181 gaaaatccac aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc
    54241 attcatacga attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga
    54301 ttggattaaa aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg
    54361 gttatgattc agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac
    54421 aaggcagaat tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta
    54481 ttggtcatac acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata
    54541 cttcccaaag tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac
    54601 ttaaaattgc ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg
    54661 ctcatctaat tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt
    54721 tgcgggtcat caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta
    54781 tttacgtcgc taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga
    54841 ttgccagcga tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg
    54901 aagagatggt taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg
    54961 tagaacgttc ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc
    55021 ctttttatat gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa
    55081 aatacgtaac agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg
    55141 atagcgatcg aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta
    55201 aacagacttt ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg
    55261 gctataacac acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg
    55321 aaacagcaga cagccgccaa gtgctggtaa aaatcaaccg tctcggctac ccttcactta
    55381 cgattacaaa tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc
    55441 atgctggacc cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac
    55501 taactttatt agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg
    55561 atatcactca tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag
    55621 attatttaga agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg
    55681 gacgtggtgc tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt
    55741 atatccaggc agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg
    55801 aagaaggaac accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag
    55861 gaaacctgaa agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat
    55921 tagaaaagga aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa
    55981 ccacagtcgt accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg
    56041 ttgacaaacc acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc
    56101 agtgtcagac atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat
    56161 ccagtccgat ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct
    56221 catcagtagg cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta
    56281 ggattcttcc ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg
    56341 gaacatccat cgcaccgttt ttatcactta cgcggatatt cactaatttt tgcggataga
    56401 ttgttacctc tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta
    56461 actgaattcc tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc
    56521 cagactgctc gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat
    56581 caccaacttg tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta
    56641 agttactcat gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt
    56701 atttagcaca gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg
    56761 caataatacg atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga
    56821 ccatctcagc taaacgttct ggatgggtag agcctacgcc gtcattgata tttaacccgt
    56881 ctggatttgt gcccattgta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac
    56941 ttgttgaagt tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt
    57001 tgatcgtttg tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag
    57061 ttcccaatcc ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt
    57121 caatttctag ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc
    57181 cattatcttg tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca
    57241 aacgtgtcag ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac
    57301 ccaccgataa aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat
    57361 cccgccctac taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac
    57421 ctccgcaccg tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc
    57481 gtacgccgtc tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt
    57541 ttctgaggaa tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt
    57601 acttgtggtc gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg
    57661 ttccgttcct gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg
    57721 actgctcgtc gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact
    57781 aactgattcg ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt
    57841 tcgatgattg atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag
    57901 caaatcgcta gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc
    57961 taccccggca gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt
    58021 gatcgaatca gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc
    58081 tgctgcattc agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga
    58141 agcgtccaca tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat
    58201 agagactttt ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt
    58261 tgaaggaatc aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt
    58321 aatagtcgat ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg
    58381 aacttcatgt gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt
    58441 atcttcgttt gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac
    58501 tgcattttca tatccatgga tatagtagcg ctttgaatca taagaaatct tgatcggcac
    58561 ccccgtaact gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc
    58621 atttgcattg aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct
    58681 tgtccgtttc tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa
    58741 gattgaatca cgtttttctt gttttttgtt ttctcttcag caattagttc tctacgcaag
    58801 attgccaaat attcttgctg ggataagcca gccatcagtt cattatctaa cgtgatgctg
    58861 acgtccccgg tttcttcaga gacaacgatg gtaatcgcgt cactgacttc gcttataccg
    58921 acagctgccc ggtgtcgagt cccgaattct ttagggatca acatactttc tgatagagga
    58981 agataggcgc ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc
    59041 ggtgtattag gaataaagat attgatcagc agctccccag tgatatctgc atctaaagca
    59101 atccctgtct caatatactc atctaatcca gtatgacgtt cgatagtgat caacgcaccg
    59161 atttttcgct tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct
    59221 tcctgctgtt cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct
    59281 ctccggattt ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg
    59341 ttcatcagcc aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata
    59401 aaaatagcta cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt
    59461 ttgtatacaa gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag
    59521 aacaagtcac cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga
    59581 aatgacataa aaagcctctc ttctcttttg agatttcctc tctagtatat catatgtttt
    59641 aattttagag gctgatatgc tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa
    59701 ttgcaagtat gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt
    59761 cattatattt tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac
    59821 gtcgttctgc tctggcagct cttctagcag ctcttctttc ttctgcttca aggtccgtcg
    59881 ttttttcttt tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct
    59941 tgattttttc taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtcgt
    60001 ggacagtctt ttcaaataaa gcttttgcaa gatttggatt tgcgttatct agcgaggaaa
    60061 agcgaacttg tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca
    60121 aagtcattgg gttcttgttt ttttcgacca ttgtagggtt atatcgatat aatgaccaat
    60181 aaccagaaga aacagcttct tttgcttctt taagcgattc tttcattccg ccaagcaagc
    60241 catgtgtgat acaaggcgta taggcaataa tcagagaagg accaggatac ttttctgctt
    60301 cttcaaatgc cttgatcgtt tgcatttgat tggcacctga agcaatttgc gccacataaa
    60361 catttccata tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag
    60421 ccgaaaattt agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg
    60481 aatagacttc gttatccaaa acaaggatat tcacgtctgc tccgctggca agtacatgat
    60541 cgatcccgcc aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt
    60601 tttttacgaa tagatccttt ctcttgtata tatcctccag ttcagggaaa attgatcgtt
    60661 cgctttcgat cgcagcttgc agttttgctg ctctttgctg cgttccttca ctctcaaaca
    60721 tgtgtactaa ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt
    60781 ctttgattga acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac
    60841 caaattcagc attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat
    60901 tggtcgtata aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat
    60961 tcgcgatcat cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc
    61021 cacagcctga gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga
    61081 cagtattctt tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat
    61141 tcacagcttg ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg
    61201 gacaagcctc tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt
    61261 attttacgcc gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca
    61321 tttcctcttc atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt
    61381 cattacacat cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct
    61441 tttccataga agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg
    61501 taccaacaga gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat
    61561 aaccagtgtt ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga
    61621 tcgcgacttg atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga
    61681 tagccattga tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct
    61741 catcaaaagg aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc
    61801 ggcgtcccaa accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt
    61861 tattttcagc caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc
    61921 tccaaagagt attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc
    61981 tatgcaggta agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg
    62041 aacgtatcgg cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg
    62101 aatcataata aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag
    62161 ctgatttatt tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg
    62221 tttgcggttc tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat
    62281 ccacgatccc tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa
    62341 aaactgctga gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa
    62401 taacagccgg gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt
    62461 ctccacctgc ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag
    62521 gcaacttttg cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac
    62581 ctgttttccg tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg
    62641 atcccataga aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat
    62701 aagaagttcc tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt
    62761 tatcataata ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg
    62821 taccactaac agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct
    62881 cctgattgat cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt
    62941 gactcgtacg gaatccatca aagaaattca aaaaagggac ttctccttca atagaagcaa
    63001 gatgggcaac aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa
    63061 atcctgtttg tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg
    63121 tcgtcactgc ccgactagca acgtggaaaa cacttggcag tagttcccca gcaatcttgt
    63181 acatattcgg tatcatcaaa agcagccctt gtgaagcggt atatgtagtt gtcagtgcac
    63241 cagttttcaa tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta
    63301 cttttactgg ttgaccgaat acgttttctt ttcctgctgc tgaccattga tctactaatt
    63361 cagccatggt cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag
    63421 aaatatatgc ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc
    63481 cttgttcata ttctctctta ttatctgttc cattataacg taaaacatgc catttttttc
    63541 ttttatttat aaaaatattt tacatgtatc tatttgaatt ggtcatctaa tttttagaca
    63601 aacagtctaa atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg
    63661 ttttaacatg gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct
    63721 ttatggttca tcgaatgctc ctaaaaaaat ggtcgaattc atcaacttag cttgtccgta
    63781 ttgccgacaa tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca
    63841 attgcagcga gtgatcaaat tgttcgataa agaaaaagaa agcttacagc gagggaacgt
    63901 gatgcaccgg tatctcacaa tcagcgatgg ccaaaaagcg atcaaagaga tcaaacaaat
    63961 attcgatact caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt
    64021 agataaacta aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga
    64081 agctgagcaa gcacatattc gatttgttcc aactgtcatc ttgggtaaag aaatctttga
    64141 tgaatctatt tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga
    64201 gcgtg
//
LOCUS       DACCCM010000007        61696 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCCM010000007 DACCCM010000000
VERSION     DACCCM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.007"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVK78_000463"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVK78_000463"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6373959.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IVK78_000464"
     CDS             complement(937..1518)
                     /locus_tag="IVK78_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6373960.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVK78_000465"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVK78_000465"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP6373961.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IVK78_000466"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IVK78_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP6373962.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IVK78_000467"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IVK78_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP6373963.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IVK78_000468"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IVK78_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6373964.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IVK78_000469"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IVK78_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6373965.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IVK78_000470"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IVK78_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP6373966.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IVK78_000471"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IVK78_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP6373967.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IVK78_000472"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IVK78_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP6373968.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IVK78_000473"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IVK78_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP6373969.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IVK78_000474"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IVK78_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP6373970.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IVK78_000475"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IVK78_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP6373971.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IVK78_000476"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IVK78_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP6373972.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IVK78_000477"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IVK78_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP6373973.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IVK78_000478"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IVK78_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP6373974.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IVK78_000479"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IVK78_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP6373975.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IVK78_000480"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IVK78_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP6373976.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IVK78_000481"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IVK78_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP6373977.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IVK78_000482"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IVK78_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP6373978.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IVK78_000483"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IVK78_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP6373979.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IVK78_000484"
     CDS             13972..14157
                     /locus_tag="IVK78_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP6373980.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IVK78_000485"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IVK78_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP6373981.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IVK78_000486"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IVK78_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP6373982.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IVK78_000487"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IVK78_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP6373983.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IVK78_000488"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IVK78_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP6373984.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IVK78_000489"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IVK78_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP6373985.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IVK78_000490"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IVK78_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP6373986.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IVK78_000491"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IVK78_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP6373987.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IVK78_000492"
     CDS             18232..18879
                     /locus_tag="IVK78_000492"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP6373988.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IVK78_000493"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IVK78_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP6373989.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IVK78_000494"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IVK78_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP6373990.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IVK78_000495"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IVK78_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP6373991.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IVK78_000496"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IVK78_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP6373992.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IVK78_000497"
     CDS             20317..21255
                     /locus_tag="IVK78_000497"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP6373993.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IVK78_000498"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IVK78_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP6373994.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IVK78_000499"
     CDS             21935..22774
                     /locus_tag="IVK78_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6373995.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IVK78_000500"
     CDS             22750..23619
                     /locus_tag="IVK78_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6373996.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IVK78_000501"
     CDS             23609..24406
                     /locus_tag="IVK78_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6373997.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IVK78_000502"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IVK78_000502"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6373998.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IVK78_000503"
     CDS             complement(25688..28357)
                     /locus_tag="IVK78_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6373999.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IVK78_000504"
     CDS             complement(28595..28783)
                     /locus_tag="IVK78_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP6374000.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IVK78_000505"
     CDS             29053..29415
                     /locus_tag="IVK78_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6374001.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IVK78_000506"
     CDS             29467..31149
                     /locus_tag="IVK78_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP6374002.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IVK78_000507"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IVK78_000507"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP6374003.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IVK78_000508"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IVK78_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP6374004.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IVK78_000509"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IVK78_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6374005.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IVK78_000510"
     CDS             34984..35988
                     /locus_tag="IVK78_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374006.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IVK78_000511"
     CDS             35989..36933
                     /locus_tag="IVK78_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374007.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IVK78_000512"
     CDS             36933..37895
                     /locus_tag="IVK78_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374008.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IVK78_000513"
     CDS             37922..38836
                     /locus_tag="IVK78_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374009.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IVK78_000514"
     CDS             38862..40643
                     /locus_tag="IVK78_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6374010.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IVK78_000515"
     CDS             40991..41677
                     /locus_tag="IVK78_000515"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6374011.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IVK78_000516"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IVK78_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP6374012.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IVK78_000517"
     CDS             45287..46096
                     /locus_tag="IVK78_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6374013.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IVK78_000518"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IVK78_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP6374014.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IVK78_000519"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IVK78_000519"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP6374015.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IVK78_000520"
     CDS             complement(49648..50100)
                     /locus_tag="IVK78_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP6374016.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IVK78_000521"
     CDS             50250..51200
                     /locus_tag="IVK78_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6374017.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IVK78_000522"
     CDS             51193..52152
                     /locus_tag="IVK78_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6374018.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IVK78_000523"
     CDS             52149..52904
                     /locus_tag="IVK78_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374019.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IVK78_000524"
     CDS             52927..53880
                     /locus_tag="IVK78_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6374020.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54403..54699)
                     /locus_tag="IVK78_000525"
     CDS             complement(54403..54699)
                     /locus_tag="IVK78_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP6374021.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55054..55410
                     /locus_tag="IVK78_000526"
     CDS             55054..55410
                     /locus_tag="IVK78_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6374022.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55420..56319
                     /locus_tag="IVK78_000527"
     CDS             55420..56319
                     /locus_tag="IVK78_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP6374023.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            56835..57566
                     /locus_tag="IVK78_000528"
     CDS             56835..57566
                     /locus_tag="IVK78_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6374024.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            57711..59738
                     /locus_tag="IVK78_000529"
     CDS             57711..59738
                     /locus_tag="IVK78_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP6374025.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            59846..60943
                     /locus_tag="IVK78_000530"
     CDS             59846..60943
                     /locus_tag="IVK78_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6374026.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            61250..>61696
                     /locus_tag="IVK78_000531"
     CDS             61250..>61696
                     /locus_tag="IVK78_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6374027.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        20386 a        11034 c        13163 g        17113 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg cttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatactc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg tccagcgttc
    54301 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt caatcagctg
    54361 accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat gtgtctcagc
    54421 aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa gcttactcca
    54481 ccgttttaat aattgataga aataactctc ttctgctttg attttatact cgcttaatat
    54541 caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca atgctttggt
    54601 tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga tattttgtgt
    54661 ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc caaagaagtc
    54721 ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac aaaaagtaat
    54781 tctttttcca atagttgtta aaggtttttt attttttata gtattttttg ttatttaaga
    54841 aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt tcaagaaact
    54901 gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat aaacgaatga
    54961 cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca tctgatgaat
    55021 aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac aaaagaagca
    55081 caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga tatcgaaaac
    55141 ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc atttcgattg
    55201 atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt ggctgataca
    55261 ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga tccatccaca
    55321 cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg cttgagaaaa
    55381 ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt ggcgatcatc
    55441 agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca gctttggcaa
    55501 gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc taataaagta
    55561 gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac gacatttcat
    55621 ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca ttttccagac
    55681 ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct tttagggatg
    55741 aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg
    55801 aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct cgaggtcaat
    55861 gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag
    55921 gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca atctgctcct
    55981 tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg agaattactt
    56041 gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg ctttgaagat
    56101 aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta tgaatggcag
    56161 ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc tatgaaatta
    56221 cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca acatttgcaa
    56281 gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag taatgtgtaa
    56341 aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt tatcgtggat
    56401 atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat gatttgtaac
    56461 tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac aaaaaaaata
    56521 taatatacat tttttattta attataatta atattgattg aagtctaggc agtcaagtca
    56581 cattttgata gataggaggg ggacttacga attattttaa aggctatgac agctgtattt
    56641 ctagattgtg tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg ctgataatca
    56701 tttataacta atcgatacat aggaccaaat ataggtctat gttcttttat ttcataagta
    56761 tcgtaaaaca gaattttgct atggattaat ccaatcgact gatatgttta gtaaataaag
    56821 aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg atgttattca
    56881 gcatggtagc aagtacgact tttgtttctg caactgaagt atacccaaag gaatataaca
    56941 cagaaggaac gattacattt gaagcaggag atgagggtgt aactcctcct gttgatccag
    57001 agaatcctga tcccaataaa cctgtcgatc catcagatcc tccaagccct ggaacaggag
    57061 gggcattatc gattgattat ggatcaaaat tcaaatttgg gactcaaaaa atttctactg
    57121 cggataaaac gtattatgca gctgctgatg tgatgaacga tgggtctaga aaaccgacat
    57181 atgtccaagt gacagatagg agaagtacct tatctgggtg gaaattaagt gtctctcaac
    57241 cagaacagtt taaaactgcc agtggggatg aactagttgg agcacagctg aaatttacga
    57301 aagggcaggc tgtttctttg gttgatccaa catatacgcc gcaaacggtg aattctgaat
    57361 taacgttgac accgggagga aacaatacgt tagcaattaa tgctaaatca ggaacaggtg
    57421 tggggacttg ggtctatcga tttggtgcaa atgaaaatga aaatcaagat gcggtacagc
    57481 tttcggtacc cggtaaatct gtcaagttag cgcaacaata ttccactaaa ttagtttgga
    57541 cattagaaga tacacctaac aactagttta ctgaaaagaa ctgttaaaaa taaatacgta
    57601 gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct aaatttgttt
    57661 ggatacgaat agttcatcta ggaatttaac gatcaggagg aaaagatata ttgaaaaaga
    57721 ggaggatcgt gctcatttta agcagtctct tagtgactaa tatctttccg atcatagggt
    57781 actctttgga attgaatcag ttggagcatg gaaatagaag agacgaagaa agcaaactca
    57841 cttcgacatt cgacagttta atagaatcca accagacaca agaaagcagt gaggatttaa
    57901 gtatttcgag tagtggagag accactgaca acgtagaagc tgagggttcg gacaaacaag
    57961 gactagaaga tgcaccaatt tctgcggttg agctgcctga cgatcccaat attatttcta
    58021 tcgataaaat ttttcaaaca cctatcggta ctagcacgtc catattggaa aatgggaagt
    58081 tactgcagtt gaacccagca gaaaggtctc aaagaggagc tatttggtca aataagaaga
    58141 ttagtttact ctcggatttt acatttaaat cttatttata cttaggaaat caacgagata
    58201 gagcgggaga tggcatgacc tttacattga ctggcgatcc tagaatggct agtaatccca
    58261 gtcaagttat tggatcgcca ggtatgggaa ttggctcata ttctactcgt tctggccaac
    58321 cttacattgc gaatgcgctc tcgattgagt ttgatacgta taaaaatagt ggttcttctg
    58381 atcgaatgga tcgagagata actgctgata atagagggca tggacatgtc gcttttgtca
    58441 cgcctaaacc aaataataat aattattctg gagagcatac tggcgtgacg gtagctccta
    58501 catttctttc taatggcaca tggcgcttgt taataattaa atgggacgcg gagcaaagaa
    58561 gtcttactta tgatttggaa ggtgtcggtc gctcttctcg gattatacct gatctcatta
    58621 ggcagtttgg ttctacagaa gtaaattggg ggtttacttc ttcaacaggt actttttttc
    58681 aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca gctgatgtaa
    58741 aagtcaacga tagtgattat ggggccatcg ctgaagctgg aggaaacgat aagataacta
    58801 tcagaaatac attgaaggtc gagggggatt ttcttgaata taagaaaatg cagactagta
    58861 tcgacctccc acaagaatta actattcccg agcatatctt gatcaatgga aatcaaattg
    58921 atgccaaaga tatcttgata accggaaatc gagtcacgct aaatctgaca gattatttta
    58981 ttgctggaga tacgttaata atcgaattgg agacaacttt gcaggaagta attcctgaaa
    59041 aagtactcac tatgcatttt gaataccttg aaaacgggaa tttgattgag caatcgaatg
    59101 aaataaacct aaagatcgca aagctgaagg agatagatat ccatgtttat tataaagata
    59161 aagacagtca gattgatatt gctgaaatga aaacaatcac tgggaaaata ggagagaagt
    59221 ataaagaaac tcctattgat attgaagggt atgtttttga ctcggattca gcgaatacag
    59281 aaggcatctt ctccgaaaat acagaggata tctattttta ttacagaaaa ggcaaacttt
    59341 atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc agtagaccat
    59401 taagtgaatt cggacagttt tcaggtgggt tgaaaattat agatgaacgg gcttttggaa
    59461 gttggaaatt acaattgaaa caagagaaag ttcttacaaa tggagatttt gagcttccag
    59521 aagctctttc ctttgttacc tctgaaaata caacggttat agggacatcc gctgttacga
    59581 ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat tctacagctc
    59641 atcgtggaat cagaatagat attcctgtag aaaaccaaag aatcggaacc tttgagggga
    59701 aactatcttg gatacttgca gatgtcccag gaaattaaca ggaagagaag aatataacaa
    59761 aattattgag tttcaagaag ggcacaggta ccataggctc ttgtgtccca cagagacaag
    59821 attttgttat agtcttctct tacatatgat taaaggagcg attttcttga gaccaaaaca
    59881 acaattattt ataacactcc tagttatttt aggggcactt tttaacaata tatcagtgtc
    59941 agctagtgca tttaattttt cagtgacgcc gataacttca gagaatcaaa tcgacaagag
    60001 aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa aagcggaatt
    60061 gagaaatgat acagaaaaag aagtgaaaat cgatatttct gtgaatagcg caaccaccaa
    60121 ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa aaagacatga gcctgatctt
    60181 tgatctggta gattatgtga gctatccaca gatagtgacg ctaaaaccta aaagtgtcca
    60241 aaccgttgct tttcatgtca ggatgccgaa tgagcgattt gatggtgtat tggctggtgg
    60301 gatcacattc caagaaatcc agacggaaaa ggaccaaaca gaaactaagg atcaaagctt
    60361 atcgattgag aatgaataca cctatattat tgctttgttg atgcaacaga cactgaacga
    60421 agttgctccg aaccttactc ttcatgaagt taaaccagat caaatcaatg caagaaatgt
    60481 catcttagca aacgttcaaa atgatcaaaa aacctatatc aatcaagtag ttattgaaac
    60541 aaaaattaca aaaaaaggac attctgaagt tttatatcaa gaggaaaaag aaggattgca
    60601 gattgcgccg aatacaaatt tttcttttcc aactgcttta aatggtcaac cgctaacccc
    60661 aggtgaatat caccttacaa tgactgtttt aggaaatgaa aatgaaaatg gaaaattttc
    60721 gagaaaaaaa ggaaatacta cgatcaacta taccaatcaa tgggtatttg aaaaagattt
    60781 tacaatagac ggaaaagtag ccaaggaatt gaacacgacg gatgttacga ttaaaaaaga
    60841 caataattgg atttatatct tagtagcttc acttttctta ttgtttattt tattatgcat
    60901 acttttgttt gttttgagaa aaaaagaaga gaaagaaaag taaggttaag ataacaaata
    60961 gtaaattttt attattgtag gatggttggg ctctttttat aacaatataa aatataccat
    61021 tttattaata aaaatgcata ctagtaaatg atataattct aataacaggt ttgatcgcga
    61081 gaaaggccaa ataaaacgct ctccaaaatg ttaaaaaaaa gacaggacga tgtatattat
    61141 acaaacaaga atcgctgaca acagtgactc ggatccagga aatcagtttt atccccagct
    61201 agttggtgaa tgcatggaaa gtaatatacg tagaaagagc tgatgactaa tgaattatga
    61261 gtttttttta agcaaagaag atcacagaag ttattcatta cttaaatatt tagaagcaag
    61321 ctcaaattta tcagagtcaa tatcgaatgt tcaagaagag ttgtcgctat cgacatttct
    61381 cttgaaaaaa acgatcgata agctgaaaca tgatttacaa aagttcgatc tagagaaaaa
    61441 tttgcgagta accgtcagtg aattggatat atgtctggaa attaatggaa aatgttctag
    61501 caaagctctg ttatctcgtt atataacgga ttctttatct atgaaaatgg tattggcttt
    61561 ctttcaggaa aaatatatct cagtagaaaa ttttgctaaa cagcatcacg tgagttattc
    61621 tgtggcttat aaagtattac aaagactaaa aagaaatcta aaaaaatatc aaattttttt
    61681 tgaaaagaga aagttg
//
LOCUS       DACCCM010000008        56347 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCCM010000008 DACCCM010000000
VERSION     DACCCM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.008"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            290..1135
                     /locus_tag="IVK78_000532"
     CDS             290..1135
                     /locus_tag="IVK78_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6374028.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IVK78_000533"
     CDS             1416..2636
                     /locus_tag="IVK78_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP6374029.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IVK78_000534"
     CDS             2633..5353
                     /locus_tag="IVK78_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6374030.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IVK78_000535"
     CDS             5337..6281
                     /locus_tag="IVK78_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6374031.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IVK78_000536"
     CDS             complement(6350..7360)
                     /locus_tag="IVK78_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6374032.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IVK78_000537"
     CDS             complement(7540..7884)
                     /locus_tag="IVK78_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6374033.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IVK78_000538"
     CDS             complement(7877..8311)
                     /locus_tag="IVK78_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP6374034.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IVK78_000539"
     CDS             8449..9186
                     /locus_tag="IVK78_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374035.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IVK78_000540"
     CDS             9179..10390
                     /locus_tag="IVK78_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374036.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IVK78_000541"
     CDS             10597..11376
                     /locus_tag="IVK78_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP6374037.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IVK78_000542"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IVK78_000542"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP6374038.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IVK78_000543"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IVK78_000543"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP6374039.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKEHLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IVK78_000544"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IVK78_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP6374040.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IVK78_000545"
     CDS             14978..16027
                     /locus_tag="IVK78_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP6374041.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IVK78_000546"
     CDS             16024..16302
                     /locus_tag="IVK78_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP6374042.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IVK78_000547"
     CDS             16277..16747
                     /locus_tag="IVK78_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP6374043.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IVK78_000548"
     CDS             16764..17066
                     /locus_tag="IVK78_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374044.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IVK78_000549"
     CDS             17083..17454
                     /locus_tag="IVK78_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP6374045.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IVK78_000550"
     CDS             17426..17785
                     /locus_tag="IVK78_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374046.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IVK78_000551"
     CDS             17957..18964
                     /locus_tag="IVK78_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6374047.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IVK78_000552"
     CDS             18989..20173
                     /locus_tag="IVK78_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP6374048.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IVK78_000553"
     CDS             complement(20610..22193)
                     /locus_tag="IVK78_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6374049.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IVK78_000554"
     CDS             complement(22771..22914)
                     /locus_tag="IVK78_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP6374050.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="IVK78_000555"
     CDS             complement(23137..23616)
                     /locus_tag="IVK78_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6374051.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="IVK78_000556"
     CDS             complement(23689..23973)
                     /locus_tag="IVK78_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374052.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="IVK78_000557"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVK78_000557"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="IVK78_000558"
     CDS             complement(24317..25603)
                     /locus_tag="IVK78_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP6374053.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="IVK78_000559"
     CDS             25741..26412
                     /locus_tag="IVK78_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP6374054.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="IVK78_000560"
     CDS             26378..26866
                     /locus_tag="IVK78_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP6374055.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="IVK78_000561"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="IVK78_000561"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP6374056.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="IVK78_000562"
     CDS             27827..28591
                     /locus_tag="IVK78_000562"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP6374057.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="IVK78_000563"
     CDS             28824..31037
                     /locus_tag="IVK78_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP6374058.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="IVK78_000564"
     CDS             31048..31494
                     /locus_tag="IVK78_000564"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP6374059.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="IVK78_000565"
     CDS             31665..32924
                     /locus_tag="IVK78_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP6374060.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="IVK78_000566"
     CDS             complement(33013..33960)
                     /locus_tag="IVK78_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6374061.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="IVK78_000567"
     CDS             34354..35646
                     /locus_tag="IVK78_000567"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP6374062.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="IVK78_000568"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="IVK78_000568"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP6374063.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="IVK78_000569"
     CDS             37539..38414
                     /locus_tag="IVK78_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6374064.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="IVK78_000570"
     CDS             38680..40563
                     /locus_tag="IVK78_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6374065.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="IVK78_000571"
     CDS             complement(40638..40922)
                     /locus_tag="IVK78_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6374066.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="IVK78_000572"
     CDS             complement(40912..41646)
                     /locus_tag="IVK78_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP6374067.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="IVK78_000573"
     CDS             41775..42398
                     /locus_tag="IVK78_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP6374068.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="IVK78_000574"
     CDS             42590..43711
                     /locus_tag="IVK78_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP6374069.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="IVK78_000575"
     CDS             43718..46846
                     /locus_tag="IVK78_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP6374070.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="IVK78_000576"
     CDS             46865..50059
                     /locus_tag="IVK78_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6374071.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="IVK78_000577"
     CDS             complement(50205..50912)
                     /locus_tag="IVK78_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6374072.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="IVK78_000578"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="IVK78_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP6374073.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="IVK78_000579"
     CDS             52407..53060
                     /locus_tag="IVK78_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP6374074.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="IVK78_000580"
     CDS             53286..54284
                     /locus_tag="IVK78_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP6374075.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="IVK78_000581"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="IVK78_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP6374076.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18538 a         9724 c        11679 g        16406 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaatg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcac
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 atttttt
//
LOCUS       DACCCM010000009        52884 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCCM010000009 DACCCM010000000
VERSION     DACCCM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.009"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVK78_000582"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVK78_000582"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP6374077.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVK78_000583"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVK78_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP6374078.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVK78_000584"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVK78_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP6374079.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVK78_000585"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVK78_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP6374080.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVK78_000586"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVK78_000586"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP6374081.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVK78_000587"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVK78_000587"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP6374082.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IVK78_000588"
     CDS             complement(8053..8523)
                     /locus_tag="IVK78_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP6374083.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IVK78_000589"
     CDS             complement(8569..8994)
                     /locus_tag="IVK78_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP6374084.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IVK78_000590"
     CDS             complement(9061..9330)
                     /locus_tag="IVK78_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP6374085.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IVK78_000591"
     CDS             complement(9341..10936)
                     /locus_tag="IVK78_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6374086.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVK78_000592"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVK78_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP6374087.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IVK78_000593"
     CDS             complement(14489..15049)
                     /locus_tag="IVK78_000593"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP6374088.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IVK78_000594"
     CDS             15402..16376
                     /locus_tag="IVK78_000594"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6374089.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IVK78_000595"
     CDS             16546..17946
                     /locus_tag="IVK78_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP6374090.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVVLETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IVK78_000596"
     CDS             complement(18024..18746)
                     /locus_tag="IVK78_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6374091.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IVK78_000597"
     CDS             complement(18825..19331)
                     /locus_tag="IVK78_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6374092.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IVK78_000598"
     CDS             19454..21253
                     /locus_tag="IVK78_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP6374093.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IVK78_000599"
     CDS             complement(21321..22895)
                     /locus_tag="IVK78_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374094.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVK78_000600"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVK78_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP6374095.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVK78_000601"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVK78_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP6374096.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IVK78_000602"
     CDS             complement(24672..25310)
                     /locus_tag="IVK78_000602"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP6374097.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IVK78_000603"
     CDS             complement(25311..25961)
                     /locus_tag="IVK78_000603"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6374098.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVK78_000604"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVK78_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP6374099.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVK78_000605"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVK78_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP6374100.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IVK78_000606"
     CDS             complement(29104..29844)
                     /locus_tag="IVK78_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP6374101.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVK78_000607"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVK78_000607"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP6374102.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IVK78_000608"
     CDS             complement(31215..32153)
                     /locus_tag="IVK78_000608"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP6374103.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVK78_000609"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVK78_000609"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6374104.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVK78_000610"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVK78_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP6374105.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IVK78_000611"
     CDS             complement(35313..35618)
                     /locus_tag="IVK78_000611"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6374106.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVK78_000612"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVK78_000612"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP6374107.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IVK78_000613"
     CDS             complement(36724..37008)
                     /locus_tag="IVK78_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374108.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IVK78_000614"
     CDS             complement(37026..37916)
                     /locus_tag="IVK78_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP6374109.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IVK78_000615"
     CDS             complement(37936..38154)
                     /locus_tag="IVK78_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374110.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IVK78_000616"
     CDS             complement(38124..39824)
                     /locus_tag="IVK78_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP6374111.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40090..40863
                     /locus_tag="IVK78_000617"
     CDS             40090..40863
                     /locus_tag="IVK78_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374112.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IVK78_000618"
     CDS             complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IVK78_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP6374113.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41579..41681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41687..42658)
                     /locus_tag="IVK78_000619"
     CDS             complement(41687..42658)
                     /locus_tag="IVK78_000619"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6374114.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IVK78_000620"
     CDS             complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IVK78_000620"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP6374115.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IVK78_000621"
     CDS             complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IVK78_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP6374116.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45613..46437)
                     /locus_tag="IVK78_000622"
     CDS             complement(45613..46437)
                     /locus_tag="IVK78_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6374117.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46410..47090)
                     /locus_tag="IVK78_000623"
     CDS             complement(46410..47090)
                     /locus_tag="IVK78_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6374118.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47113..48051)
                     /locus_tag="IVK78_000624"
     CDS             complement(47113..48051)
                     /locus_tag="IVK78_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP6374119.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48296..49147)
                     /locus_tag="IVK78_000625"
     CDS             complement(48296..49147)
                     /locus_tag="IVK78_000625"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP6374120.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49301..51874)
                     /gene="mprF"
                     /locus_tag="IVK78_000626"
     CDS             complement(49301..51874)
                     /gene="mprF"
                     /locus_tag="IVK78_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6374121.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52015..52821
                     /gene="proC"
                     /locus_tag="IVK78_000627"
     CDS             52015..52821
                     /gene="proC"
                     /locus_tag="IVK78_000627"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP6374122.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT        14661 a        11479 c         9505 g        17239 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggtccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcctg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttagtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctga ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcgcgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgt gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacatccttt aaataaaaaa tacaaaaatc acaaaaatta
    39961 attgaaaatg agagtgtttt caatgaaaac gtgattttgt aatggaattt taaaataaaa
    40021 gaaacaagct tttttaatgc aaatgttgta aaataaaaga gattaagagg aaataagagg
    40081 aggaacatga tgactgagtc agtttatgta catattgata ccacaagtaa tgctgtttta
    40141 acaaaagggc tgactgctac tgactttgca aacagtatcg tgcattttcc tcaaaacttg
    40201 ctcttgcttg atccatctgc ttctgctgga gaatatgaat cccatacagg gctgaaagta
    40261 atccgtggta cagaaaatat tcagcgtttc ttttcctctt ctcgtaacag gcgtggattc
    40321 gatgaattga agtggatcga ctttactgat ttaacgatgc tgaaagaatt gacaccttta
    40381 gaaatttcag aattattata ttttggtcat atgaaaacac atcttcattc gccgtttttt
    40441 tataaactac agaataattt tgtatacttc gatcttaatg atcagctgaa tcggatttat
    40501 tatcgctatt tagatgaatt ttataggatt tttgctagta aattaagttc gatcttgtca
    40561 gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga aaaaatcagt
    40621 gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt ttcttttgat
    40681 caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga agattatatt
    40741 tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt aatctactct
    40801 gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct gccattgaag
    40861 tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat gattctgtct
    40921 caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac aaaaatcgaa
    40981 ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt actcaacccg
    41041 ctggcaccat tcattacgaa agaaaagagc caagaattca tcccccatag tgcatacgct
    41101 ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc gccaatcaat
    41161 gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata gcctttttgg
    41221 acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg gtactcaatc
    41281 aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag gatgccccaa
    41341 atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag agtcaatgga
    41401 atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa cgacaacacc
    41461 atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc cataaaaaaa
    41521 cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa tagtcaaaca
    41581 gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg gtttagaaat
    41641 agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat ttattttttg
    41701 tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca tctcccatta
    41761 gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga agatagatcg
    41821 agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa gcagggccgg
    41881 ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct ttcaaagcat
    41941 tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt acacaagttt
    42001 tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt ggacggcgtt
    42061 tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc cgtgtgacac
    42121 caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt tctaagaaat
    42181 aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag aaaccttgga
    42241 tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt tgaatcatgt
    42301 ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct tgacgcgcat
    42361 agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc agtgcgtcaa
    42421 tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat tgaataacat
    42481 agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca tcactaaatt
    42541 gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc tctgctaatg
    42601 ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt tttgacatgg
    42661 ggacctccac tttctttttc acaactctag aattctacct aataaatagt agtcatattt
    42721 tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt ttttttgcat
    42781 atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct ggaatattgt
    42841 ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca ggtccgatga
    42901 tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt ttgttctttc
    42961 catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc agcgtagcat
    43021 caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca tttttcactt
    43081 ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt aaatgtgcat
    43141 aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag ttttcaatga
    43201 cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg acacaatcag
    43261 aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg tctggaccga
    43321 tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg ccattgacca
    43381 tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac gcgatccgat
    43441 cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact gtatggcctt
    43501 cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca ttgtttgttc
    43561 caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt cctgtattga
    43621 tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt ttttctacga
    43681 tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct tgtgccgtca
    43741 agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc agcgtttcag
    43801 tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt ccttccaaga
    43861 aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt aacgcatatt
    43921 tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg atcgtaatca
    43981 cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata ggtttaccag
    44041 aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc cctgccgcca
    44101 aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg ctgatttcag
    44161 tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat cccagcgttt
    44221 tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc ggtgctctcc
    44281 tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg ttttttgaaa
    44341 tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta aacgtttttc
    44401 aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc aatttttccg
    44461 tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat aaacggtgtt
    44521 caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa gccattttca
    44581 caaaattttt cttactactc gaaactaagc ggctttttca caacctctta ttctgaaaaa
    44641 taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt gacataaagc
    44701 agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc aaatacagtg
    44761 atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt gactgttccg
    44821 ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa acttctctta
    44881 gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac acttactgtt
    44941 gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt cagataatag
    45001 gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc atcgattttt
    45061 tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc aggttctcct
    45121 aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc tgatagacgt
    45181 tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg gatgaacaat
    45241 acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc tttaaatgtt
    45301 ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata acgttcggca
    45361 aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt catgtcaact
    45421 agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat tttatcgttt
    45481 tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata agaaaatagt
    45541 tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta taaggcttct
    45601 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
    45661 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
    45721 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
    45781 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
    45841 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
    45901 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
    45961 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
    46021 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
    46081 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
    46141 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
    46201 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
    46261 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
    46321 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
    46381 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
    46441 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
    46501 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
    46561 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
    46621 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
    46681 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
    46741 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
    46801 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
    46861 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
    46921 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
    46981 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
    47041 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
    47101 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
    47161 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
    47221 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
    47281 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
    47341 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
    47401 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
    47461 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
    47521 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
    47581 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
    47641 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
    47701 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
    47761 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
    47821 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
    47881 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
    47941 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
    48001 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
    48061 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
    48121 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
    48181 aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat aaaaaactga
    48241 agaataccat tttaggattc ctcagttttt catcacctat tttacttctt atattttatt
    48301 gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta tatactctct
    48361 gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg
    48421 cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact ggatgccgct
    48481 ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga taatcttgct
    48541 ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc gtatctactt
    48601 ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca acccagcact
    48661 gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat gccgtacttc
    48721 cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc agttcttcta
    48781 acgaacagcc cagattccat aattggttga tccctcttag agcagctgca cagtctgtac
    48841 ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc ttgacaccgg
    48901 ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg ttccgttcat
    48961 ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt atttcttcaa
    49021 aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca tgatacccat
    49081 cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct ttttcaatca
    49141 attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca tagggaaaaa
    49201 taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa cagctttcac
    49261 agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct gtgagcactt
    49321 cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt agctgcatgg
    49381 tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc caatgatcga
    49441 catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta ccataattat
    49501 atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca acattagcta
    49561 atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca ataaacagat
    49621 tggctaacag atagtcagct aattcctcag attctttagg taagacccgc aacaaatcat
    49681 atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact ttttgatctt
    49741 tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt cccacagaga
    49801 agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca gcttgatgta
    49861 attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc aaacgttgat
    49921 attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca atccctgctt
    49981 cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag ctttctgata
    50041 tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac tgattcgttg
    50101 ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat ttgtttcctt
    50161 tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa aaataactgt
    50221 aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt
    50281 caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct aaatattgat
    50341 aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag aaccacacat
    50401 catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa aagcgtccgc
    50461 tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta gcatcaaaga
    50521 ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg gataaccaca
    50581 cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta cgtgtgattg
    50641 tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt ttgactttta
    50701 ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc actgtctgat
    50761 ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt gcagacagat
    50821 tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca cttacgagca
    50881 cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat cgattgattt
    50941 tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga acataataaa
    51001 acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac aattgactca
    51061 ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc gtcagcattc
    51121 ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata tctattctca
    51181 tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca aaggatgcca
    51241 gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt ctctttagat
    51301 aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag atccaatagc
    51361 ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga aacgaccata
    51421 cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa accatttttc
    51481 tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca ccaccaaaac
    51541 cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct tttctttcca
    51601 tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac aacaacatcg
    51661 gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc
    51721 ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg acttgatttg
    51781 ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta tgttccttta
    51841 tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa cttgtatact
    51901 tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta gacctgttct
    51961 ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga taacatgaaa
    52021 gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt attaaaaaaa
    52081 ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa
    52141 tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc atcacaatca
    52201 gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga agaactaaga
    52261 gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt aacagattta
    52321 gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc
    52381 agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt
    52441 cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc agaatcagat
    52501 ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat gctgatcgat
    52561 gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac tgagattgct
    52621 gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa
    52681 cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt tgccttagaa
    52741 gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga gaaagatctt
    52801 gagatgttga ataaaaaata atgatagcaa tgttttttcg taccttacaa aaaaagaaga
    52861 gctgtggctt ttgccgcggc tctt
//
LOCUS       DACCCM010000010        48493 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCCM010000010 DACCCM010000000
VERSION     DACCCM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.010"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(23..2095)
                     /locus_tag="IVK78_000628"
     CDS             complement(23..2095)
                     /locus_tag="IVK78_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6374123.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IVK78_000629"
     CDS             complement(2028..2405)
                     /locus_tag="IVK78_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP6374124.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IVK78_000630"
     CDS             complement(2534..3094)
                     /locus_tag="IVK78_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6374125.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IVK78_000631"
     CDS             3244..4536
                     /locus_tag="IVK78_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6374126.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IVK78_000632"
     CDS             complement(4596..5714)
                     /locus_tag="IVK78_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP6374127.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVK78_000633"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVK78_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP6374128.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVK78_000634"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVK78_000634"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP6374129.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IVK78_000635"
     CDS             8920..9519
                     /locus_tag="IVK78_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP6374130.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVK78_000636"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVK78_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP6374131.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IVK78_000637"
     CDS             complement(11214..11558)
                     /locus_tag="IVK78_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP6374132.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IVK78_000638"
     CDS             complement(11695..12831)
                     /locus_tag="IVK78_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6374133.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IVK78_000639"
     CDS             complement(13020..13994)
                     /locus_tag="IVK78_000639"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6374134.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IVK78_000640"
     CDS             complement(14074..14949)
                     /locus_tag="IVK78_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP6374135.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IVK78_000641"
     CDS             15079..16986
                     /locus_tag="IVK78_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP6374136.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IVK78_000642"
     CDS             complement(17527..20805)
                     /locus_tag="IVK78_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP6374137.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IVK78_000643"
     CDS             complement(21076..21621)
                     /locus_tag="IVK78_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6374138.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IVK78_000644"
     CDS             complement(21633..22325)
                     /locus_tag="IVK78_000644"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP6374139.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IVK78_000645"
     CDS             complement(22344..22628)
                     /locus_tag="IVK78_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374140.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IVK78_000646"
     CDS             complement(22641..23204)
                     /locus_tag="IVK78_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6374141.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IVK78_000647"
     CDS             23432..24094
                     /locus_tag="IVK78_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP6374142.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IVK78_000648"
     CDS             24218..25414
                     /locus_tag="IVK78_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP6374143.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IVK78_000649"
     CDS             complement(25515..26891)
                     /locus_tag="IVK78_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6374144.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IVK78_000650"
     CDS             complement(26928..27794)
                     /locus_tag="IVK78_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP6374145.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IVK78_000651"
     CDS             complement(27883..28908)
                     /locus_tag="IVK78_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6374146.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IVK78_000652"
     CDS             complement(28926..29600)
                     /locus_tag="IVK78_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6374147.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IVK78_000653"
     CDS             complement(29784..30677)
                     /locus_tag="IVK78_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6374148.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IVK78_000654"
     CDS             complement(30751..32448)
                     /locus_tag="IVK78_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6374149.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IVK78_000655"
     CDS             complement(32441..33754)
                     /locus_tag="IVK78_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6374150.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37272)
                     /locus_tag="IVK78_000656"
     CDS             complement(33754..37272)
                     /locus_tag="IVK78_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6374151.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37565..38023)
                     /locus_tag="IVK78_000657"
     CDS             complement(37565..38023)
                     /locus_tag="IVK78_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6374152.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38321..38839)
                     /locus_tag="IVK78_000658"
     CDS             complement(38321..38839)
                     /locus_tag="IVK78_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6374153.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(38841..39458)
                     /locus_tag="IVK78_000659"
     CDS             complement(38841..39458)
                     /locus_tag="IVK78_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6374154.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39455..40309)
                     /locus_tag="IVK78_000660"
     CDS             complement(39455..40309)
                     /locus_tag="IVK78_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6374155.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40306..41217)
                     /locus_tag="IVK78_000661"
     CDS             complement(40306..41217)
                     /locus_tag="IVK78_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6374156.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41258..42592)
                     /locus_tag="IVK78_000662"
     CDS             complement(41258..42592)
                     /locus_tag="IVK78_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6374157.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42800..43630
                     /locus_tag="IVK78_000663"
     CDS             42800..43630
                     /locus_tag="IVK78_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP6374158.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43874..44123)
                     /locus_tag="IVK78_000664"
                     /pseudo
     CDS             complement(43874..44123)
                     /locus_tag="IVK78_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44314..44508)
                     /locus_tag="IVK78_000665"
     CDS             complement(44314..44508)
                     /locus_tag="IVK78_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374159.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45013..45198)
                     /locus_tag="IVK78_000666"
     CDS             complement(45013..45198)
                     /locus_tag="IVK78_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374160.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46048..46389)
                     /locus_tag="IVK78_000667"
     CDS             complement(46048..46389)
                     /locus_tag="IVK78_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6374161.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46389..46619)
                     /locus_tag="IVK78_000668"
     CDS             complement(46389..46619)
                     /locus_tag="IVK78_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6374162.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            46906..47244
                     /locus_tag="IVK78_000669"
     CDS             46906..47244
                     /locus_tag="IVK78_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374163.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47449..47688)
                     /locus_tag="IVK78_000670"
     CDS             complement(47449..47688)
                     /locus_tag="IVK78_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP6374164.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(47664..48011)
                     /locus_tag="IVK78_000671"
     CDS             complement(47664..48011)
                     /locus_tag="IVK78_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP6374165.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14115 a        10334 c         8478 g        15566 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaaca cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tatgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    37561 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    37621 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    37681 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    37741 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    37801 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    37861 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    37921 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    37981 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    38041 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    38101 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    38161 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    38221 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    38281 atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata
    38341 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    38401 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    38461 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    38521 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    38581 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    38641 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    38701 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    38761 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    38821 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    38881 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    38941 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    39001 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    39061 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    39121 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg ataatcgatc
    39181 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaaa agagaagttc
    39241 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    39301 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    39361 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    39421 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    39481 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    39541 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    39601 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    39661 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    39721 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    39781 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    39841 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    39901 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    39961 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    40021 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    40081 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    40141 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    40201 tccaaagcaa tggaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    40261 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    40321 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    40381 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    40441 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    40501 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    40561 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    40621 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    40681 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    40741 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    40801 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    40861 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    40921 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    40981 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    41041 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    41101 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    41161 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    41221 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    41281 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    41341 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    41401 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    41461 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    41521 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    41581 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    41641 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    41701 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    41761 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    41821 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    41881 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    41941 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    42001 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    42061 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    42121 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    42181 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    42241 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    42301 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    42361 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    42421 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    42481 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    42541 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    42601 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    42661 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    42721 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    42781 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    42841 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    42901 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    42961 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    43021 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    43081 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    43141 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    43201 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    43261 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    43321 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    43381 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    43441 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    43501 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    43561 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    43621 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    43681 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    43741 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    43801 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    43861 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    43921 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    43981 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    44041 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    44101 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    44161 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    44221 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    44281 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    44341 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    44401 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    44461 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    44521 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    44581 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    44641 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    44701 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    44761 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    44821 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    44881 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    44941 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    45001 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    45061 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    45121 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    45181 tttagaattt ttactcattc atagacactt ccttaaataa cttttattat agaaggtatt
    45241 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaacttaca ttatactgga
    45301 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
    45361 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
    45421 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
    45481 gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa aaactagcgg
    45541 tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg caaatttctc
    45601 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
    45661 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
    45721 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
    45781 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
    45841 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
    45901 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc atcgaccaca
    45961 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
    46021 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
    46081 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
    46141 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
    46201 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
    46261 acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc
    46321 tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc
    46381 ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca
    46441 taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt
    46501 gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta
    46561 tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt
    46621 atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt
    46681 gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata
    46741 aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac attgaaccta
    46801 tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa ttttgcagat
    46861 aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc cgattataaa
    46921 caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt cacgaaacta
    46981 gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat agaaacattg
    47041 gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat cgtagctatt
    47101 tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa atggcaacct
    47161 cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt gaattttaga
    47221 ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa aaaagactcg
    47281 cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt gaccctatag
    47341 atgcagtata tgcagcttat gagcatttct tctgttctct ttgtttttgg gatctgtgct
    47401 gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt ataccttctc
    47461 cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa tatctgttac
    47521 ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga ttggtgcgac
    47581 tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat cctgatggct
    47641 acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa atttgtcttt
    47701 ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg atgaaaggtc
    47761 tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt tttaaatccg
    47821 gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt tcttaacgtc
    47881 acaacaagat ttgacggaaa aacttctcca gctgctaatt ctgctgcctg cttcggttgt
    47941 ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc aaaaaaataa
    48001 cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca acaagaagtt
    48061 cgcaccaaca aaacactttc aatttttgta gaactaaaaa agttgtgaca agtgacaaac
    48121 tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat attcaacaaa
    48181 catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg caggcttacg
    48241 acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    48301 aatgtagaaa gctattttca caaaattttt cttaatactc gaaactaagc gactttttca
    48361 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
    48421 gaaaaatatg agcagctatt tttatttggc tgttcttatt acttgatgca gggcagcttt
    48481 ttcacaacct ctc
//
LOCUS       DACCCM010000011        46148 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCCM010000011 DACCCM010000000
VERSION     DACCCM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.011"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            46..2211
                     /gene="acm"
                     /locus_tag="IVK78_000672"
     CDS             46..2211
                     /gene="acm"
                     /locus_tag="IVK78_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP6374166.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2483..2968)
                     /locus_tag="IVK78_000673"
     CDS             complement(2483..2968)
                     /locus_tag="IVK78_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP6374167.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3448..4089
                     /locus_tag="IVK78_000674"
     CDS             3448..4089
                     /locus_tag="IVK78_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6374168.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4108..4743
                     /locus_tag="IVK78_000675"
     CDS             4108..4743
                     /locus_tag="IVK78_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6374169.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4761..5576
                     /locus_tag="IVK78_000676"
     CDS             4761..5576
                     /locus_tag="IVK78_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6374170.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5895..7331
                     /locus_tag="IVK78_000677"
     CDS             5895..7331
                     /locus_tag="IVK78_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6374171.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7520..9967
                     /locus_tag="IVK78_000678"
     CDS             7520..9967
                     /locus_tag="IVK78_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6374172.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10197..11264
                     /gene="rlmN"
                     /locus_tag="IVK78_000679"
     CDS             10197..11264
                     /gene="rlmN"
                     /locus_tag="IVK78_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6374173.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11457..12560)
                     /locus_tag="IVK78_000680"
     CDS             complement(11457..12560)
                     /locus_tag="IVK78_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6374174.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13113..13661
                     /gene="aac(6')"
                     /locus_tag="IVK78_000681"
     CDS             13113..13661
                     /gene="aac(6')"
                     /locus_tag="IVK78_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6374175.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14206..15867
                     /locus_tag="IVK78_000682"
     CDS             14206..15867
                     /locus_tag="IVK78_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6374176.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15937..16284)
                     /locus_tag="IVK78_000683"
     CDS             complement(15937..16284)
                     /locus_tag="IVK78_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP6374177.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16368..17309
                     /locus_tag="IVK78_000684"
     CDS             16368..17309
                     /locus_tag="IVK78_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374178.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKTQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17310..18353
                     /locus_tag="IVK78_000685"
     CDS             17310..18353
                     /locus_tag="IVK78_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374179.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18359..19429
                     /locus_tag="IVK78_000686"
     CDS             18359..19429
                     /locus_tag="IVK78_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374180.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19419..20372
                     /locus_tag="IVK78_000687"
     CDS             19419..20372
                     /locus_tag="IVK78_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374181.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20384..21394
                     /locus_tag="IVK78_000688"
     CDS             20384..21394
                     /locus_tag="IVK78_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6374182.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21679..22500)
                     /locus_tag="IVK78_000689"
     CDS             complement(21679..22500)
                     /locus_tag="IVK78_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6374183.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22597..22875)
                     /locus_tag="IVK78_000690"
     CDS             complement(22597..22875)
                     /locus_tag="IVK78_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374184.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23143..25152
                     /gene="metG"
                     /locus_tag="IVK78_000691"
     CDS             23143..25152
                     /gene="metG"
                     /locus_tag="IVK78_000691"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP6374185.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25631..26218)
                     /locus_tag="IVK78_000692"
     CDS             complement(25631..26218)
                     /locus_tag="IVK78_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374186.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26393..27715
                     /locus_tag="IVK78_000693"
     CDS             26393..27715
                     /locus_tag="IVK78_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6374187.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27870..28646
                     /locus_tag="IVK78_000694"
     CDS             27870..28646
                     /locus_tag="IVK78_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6374188.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28639..29208
                     /gene="rnmV"
                     /locus_tag="IVK78_000695"
     CDS             28639..29208
                     /gene="rnmV"
                     /locus_tag="IVK78_000695"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP6374189.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29201..30085
                     /gene="rsmA"
                     /locus_tag="IVK78_000696"
     CDS             29201..30085
                     /gene="rsmA"
                     /locus_tag="IVK78_000696"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP6374190.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30536..31165
                     /locus_tag="IVK78_000697"
     CDS             30536..31165
                     /locus_tag="IVK78_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374191.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31289..32398)
                     /gene="ugpC"
                     /locus_tag="IVK78_000698"
     CDS             complement(31289..32398)
                     /gene="ugpC"
                     /locus_tag="IVK78_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP6374192.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32607..33029
                     /gene="mgsA"
                     /locus_tag="IVK78_000699"
     CDS             32607..33029
                     /gene="mgsA"
                     /locus_tag="IVK78_000699"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP6374193.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33352..33888
                     /locus_tag="IVK78_000700"
     CDS             33352..33888
                     /locus_tag="IVK78_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6374194.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33928..35691)
                     /locus_tag="IVK78_000701"
     CDS             complement(33928..35691)
                     /locus_tag="IVK78_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374195.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35694..37409)
                     /locus_tag="IVK78_000702"
     CDS             complement(35694..37409)
                     /locus_tag="IVK78_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374196.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38188..38412)
                     /locus_tag="IVK78_000703"
     CDS             complement(38188..38412)
                     /locus_tag="IVK78_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374197.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38612..41380
                     /locus_tag="IVK78_000704"
     CDS             38612..41380
                     /locus_tag="IVK78_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6374198.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            41461..41880
                     /locus_tag="IVK78_000705"
     CDS             41461..41880
                     /locus_tag="IVK78_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP6374199.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41892..42362
                     /locus_tag="IVK78_000706"
     CDS             41892..42362
                     /locus_tag="IVK78_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6374200.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42379..43158
                     /locus_tag="IVK78_000707"
     CDS             42379..43158
                     /locus_tag="IVK78_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6374201.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43145..43966
                     /locus_tag="IVK78_000708"
     CDS             43145..43966
                     /locus_tag="IVK78_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6374202.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            43983..45032
                     /locus_tag="IVK78_000709"
     CDS             43983..45032
                     /locus_tag="IVK78_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6374203.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45045..46049
                     /locus_tag="IVK78_000710"
     CDS             45045..46049
                     /locus_tag="IVK78_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6374204.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT        15257 a         8049 c         9519 g        13323 t
ORIGIN      
        1 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
       61 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      121 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      181 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      241 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      301 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      361 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      421 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      481 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      541 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      601 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      661 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      721 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      781 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      841 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      901 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
      961 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1021 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1081 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1141 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1201 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1261 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1321 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1381 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1441 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1501 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1561 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1621 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1681 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1741 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1801 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1861 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1921 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     1981 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2041 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2101 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2161 atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat
     2221 aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa
     2281 aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta
     2341 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
     2401 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
     2461 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
     2521 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
     2581 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
     2641 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
     2701 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
     2761 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
     2821 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
     2881 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
     2941 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
     3001 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
     3061 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
     3121 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
     3181 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
     3241 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
     3301 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     3361 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     3421 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     3481 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     3541 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     3601 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     3661 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     3721 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     3781 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     3841 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     3901 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     3961 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat
     4021 cttacattga ctggtattct aacaatcgta atgaaacaag ttgaaggaaa attaaattat
     4081 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     4141 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     4201 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     4261 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     4321 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     4381 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     4441 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     4501 ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg
     4561 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     4621 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     4681 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     4741 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     4801 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     4861 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     4921 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     4981 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     5041 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     5101 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     5161 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     5221 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     5281 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     5341 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     5401 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     5461 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     5521 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     5581 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     5641 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     5701 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     5761 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacatatttt
     5821 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     5881 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     5941 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     6001 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     6061 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     6121 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     6181 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     6241 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     6301 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     6361 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     6421 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     6481 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     6541 aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     6601 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     6661 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     6721 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     6781 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     6841 ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     6901 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     6961 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     7021 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     7081 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     7141 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     7201 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     7261 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     7321 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     7381 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     7441 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     7501 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     7561 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     7621 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     7681 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     7741 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     7801 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     7861 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     7921 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     7981 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     8041 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     8101 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     8161 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     8221 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     8281 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     8341 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     8401 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     8461 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     8521 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     8581 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     8641 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     8701 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     8761 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     8821 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     8881 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     8941 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     9001 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     9061 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     9121 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     9181 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     9241 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     9301 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     9361 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     9421 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     9481 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     9541 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     9601 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     9661 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     9721 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     9781 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     9841 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     9901 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
     9961 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
    10021 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
    10081 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
    10141 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
    10201 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
    10261 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
    10321 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
    10381 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
    10441 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
    10501 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
    10561 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
    10621 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
    10681 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
    10741 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
    10801 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
    10861 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
    10921 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
    10981 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
    11041 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
    11101 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
    11161 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
    11221 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
    11281 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
    11341 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
    11401 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
    11461 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
    11521 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
    11581 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
    11641 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
    11701 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
    11761 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
    11821 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
    11881 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
    11941 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
    12001 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
    12061 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
    12121 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
    12181 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
    12241 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
    12301 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    12361 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    12421 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    12481 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    12541 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    12601 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    12661 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    12721 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    12781 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    12841 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    12901 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    12961 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
    13021 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
    13081 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
    13141 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
    13201 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
    13261 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
    13321 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
    13381 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
    13441 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
    13501 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
    13561 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
    13621 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
    13681 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg
    13741 aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag aggttgtgaa
    13801 aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg
    13861 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttattc agataaggga
    13921 ctgtgacaag acttttgtca cagtttcttt ttgtttattc ttttttatct ttgtatggca
    13981 ataatagaca atataacgaa aattatttaa tggcttttgg aagaccatgg aaaaccttgt
    14041 acaaagctat tttttctgaa aaattccttg aaacctcacg aaaaaatcat tattttatga
    14101 aaaagacaaa aaaagtgttg attttttaat aattattcat taaactctta aacaacttca
    14161 gaaaatatta attattctga aaattccaat aaaggggtac gtaaaatgaa aaagaaattt
    14221 acatttggtt tggttgcggt atgcggcctt gtacttgctg gatgttatgg cggcagttca
    14281 gccacaagca gtagttcagc aaaaagcggt tcagcagatg gaggaggcgt atttaactta
    14341 gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg caacagatac aatcagtttt
    14401 acagcattga ataacgttta tgaaggaatt taccgtcttg atgaagacag caaaccacag
    14461 ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg gattaactta caaaatcaaa
    14521 ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta cagcagcgga ttacgtttat
    14581 ggctggcaac gaacagtaga cccagctaca gcttctgaat atgcttacct gtatgcgcca
    14641 gttgaaaatg cagaagacat tacagctgga aagaaagata aatcagaact agggatcaaa
    14701 gcagttggtg actatgaatt agaaatcaaa ttgacgaaac aaacaccata tttccaatat
    14761 ctgcttgctt tcccatcatt tttcccacaa aaacaatccg tagtggaaga aaatggggat
    14821 gcctatgcat ctgcaagtga taaagctgtt tataatggac cattcacatt agaagatttt
    14881 gatggaccag gaacagatac agaatggact tataagaaaa atgatcatta ctgggacaaa
    14941 gatacagtaa aactttctga aatcaaagtc agtgtagtaa aagaatcttc tactgcattg
    15001 aacttattca aagatggcca agcagatgac gtgatcttgt ctggtgagtt agcacagcaa
    15061 aacgcaaacg atccagctta tacttctgtt aaagaagcgc ggacaagcta tatcgaattc
    15121 aatcaacgtg aaaagaattc accattcaac aatgtgaacc taagaaaagc tatttcttat
    15181 tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg acggatcagt tgtctctaca
    15241 ggattgatcc catcagatat ggtaaaaagt ccaacaacga acgaagactt tgcaaaagaa
    15301 gctggaaaat tagtttctta tgacaaagat aaagcaaaag aatattggga aaaagcgaaa
    15361 aaagaattgg gcgttgattc tttagaattt gatttgatgg cttctgatga tgattcatcg
    15421 aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa acttggatgg cgtaacagtc
    15481 aaaccaacac ctgttccatt ctctgtacgt cttgaccgct caacttcggg tgattttgac
    15541 gcagtactag gcggatgggc tgctgactat gcagacccaa gcagctttac tgatttgttt
    15601 gtcacaggta attcatacaa ccgtggtcgc tggagcaatg cagaatatga cgaagcagtt
    15661 aaagcagctt catcaacaga cgctggcgat gaacaagccc gttgggatga tttccaaaaa
    15721 gcaatcaaga tcatctctga tgatatgggt gtcgttccgg tgtaccaaaa agcagaaggt
    15781 cacctagtga atgaaaaaat caaaggtatc gttcaccatg cggcaggcgc ttcatgggat
    15841 tacaaatgga catacgttga agaataatcg ttaataccgt gagcatgaga aaaaacgagg
    15901 ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat cctaacaaag aaaaaagcat
    15961 gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc agccaaaagc tgtacatacc
    16021 gtagctggca gacgagagcg aggaaaattc tggttttttc tttcggttcc ttgttcgtgc
    16081 aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa gaaaagaccc ataaaaaacc
    16141 gccgataatc agaaacggca atgaccataa gaatagatca ttagataatt gaatgagtgt
    16201 aaaggaatgg ttgatgagat tttttattaa aataaccaga atcagtagaa aagctactgc
    16261 gtaggggata gatttttttt tcatttgatt gctcctttat ttttggttca ttatctaata
    16321 atgtagcgaa acatctgtca gaagtcaaaa gctaggggga aacattagtg aatagttata
    16381 tcaaatatgt attaaaacgt gtctttttca tgatcatcac gctttggtta attgcaacaa
    16441 tcaccttttt cttgatgcaa ttgctgccgg gcacgccata tacgaatcag gaacgtttga
    16501 gtcctgaaac aatcgagatg ctaaataaac aagtcggttt ggataaacca gttatcgtac
    16561 aatatgggat ttatctttct aatttgcttc aaggagattt tggaatttcc ttccaattta
    16621 aaacccagcc agtcgcacat ttgttagctg gaagaatcgg accatccttg cagttagggt
    16681 tacaggcaat tatctttgga acagtcctag ggacgatttt aggaacgatc tctgccatga
    16741 agcaaaatac atgggcagat acatcttcta cactcgtagc gatattaggt cgttccattc
    16801 caaactttgt atttgctgtt ttattacaat atatctttgc aatcaaatta caagttttgc
    16861 caatcgcaaa atgggatggc tttgtgtaca caatcttacc aacaattgcg ttggccatgt
    16921 cgccgcttgc tgactctgct cggtttatcc gtacggaaat ggtggaagta ctgcatagtg
    16981 attatgtgga attagcaaaa gcaaaaggat tgagccgttg ggaaatcgcc tttaaacatg
    17041 gtcttcgaaa tagtttgatt ccattgatga ctttattagg accgttagct gtagcattga
    17101 tgaccggatc actggtggta gaaaatatct ttgctattcc tggtatcggg gaacaattcg
    17161 ttaagtcaat cacaacaaat gattatccta cgatcatggc tgtaacgatc ctttactcat
    17221 ttatgctgat ttttgttatt ttgatcgtgg atctattgta cggtctagtt gatccaagaa
    17281 ttcgtgtttc agaagggagc cgaggataaa tggaaatgat accaaagcgt tatgaaacag
    17341 ttgcagatat ccctgcggat gaatttcttc cattacaaaa aaataccgaa gaagaacgtg
    17401 aacagattac tgcaccttct ttgaatttta tccaagattc atggcgccgt ttaaagaaaa
    17461 acaaagctgc cgttatttca atggttttac tattggtcat cattttgatc tctgtgatca
    17521 caatctttgt ttctcctcat gatccaactg cccaaaacgt ggcttatatc aatttgcctc
    17581 ctcgtattcc gggaatcaat atcgatggat tgaatggaaa agcaatggtt ggtggtgaac
    17641 ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt aaatttcttt ttaggaacag
    17701 acggtttggg acgtgatgta ttaagccgct tatttatggg aatgagaatc tcgttgctga
    17761 tcgcatttat tgctgcatta ctagatgtaa cgattggtgt agcttacggg ttggtttctg
    17821 gtttgttagg cggacgtgtc gataacgtga tgcaacgttt tttggaaatc ttatcaggaa
    17881 ttccaaacct cgttgtcatg attttgatgt tggtcgtttt tgaaccagga attttttcaa
    17941 tcgtggctgc tatggccatc acaaactgga taccaatggc acgtatcgtt cgtgcacaaa
    18001 caatgaaatt aaaagatcaa gaatatgttt tagctggatt gacattaggt gaatcaagaa
    18061 tgaaaattgc attcaaacat attttgccga atatctctag tgtcattatt attcaaatga
    18121 tgtttagtat tccatctgct attttcttcg aagccttttt aagctttatc ggtttaggtc
    18181 ttacgcctcc gtcagcttca ttaggaacga tgttgagcga agggtacaag acattcctgt
    18241 atctgcctta tctattatgg attccggcag taactttatc ggttatcatg attggcttta
    18301 acttacttgc tgatggattg cgtgatgcct ttgatcctaa aatgaaagag tgaagacgat
    18361 gacgaaaatt ttagaagtga aagacttaga aatctctttt gatacgtatg ctggaaaggt
    18421 acgagcgatt cgcggtgtca attttcattt gaataaagga gaaacattgg cgatcgttgg
    18481 ggaatctgga agtggaaaat cagtgacgac cagaagcatc atgcgcttgc tgtcaagcaa
    18541 cgccaatata gatgctggtc aaatcctctt taaaggtaac gatatcgttg gaaaatcaga
    18601 aagagagatg caaaaaatcc gtggtaaaga aatagcgatg attttccaag atccaatgac
    18661 ctctttagat ccgacgatgc ccattgggaa gcaagtcgct gagtccctta gaaagcataa
    18721 taaagtttca aaaaaagagg gacaaaaagc tgcattagaa ttgcttaaat tagttggaat
    18781 acccgaagct gagaaacgta tcaacagtta tccgcatcag ttctcaggag gtcagcgtca
    18841 acggatcgtg atcgcgattg cactggtatg ttatccggaa atcctgattg ctgatgaacc
    18901 tacaacagcg cttgatgtga ctattcaagc acagattttg gagctgctga aagaaattca
    18961 gacaaaaatc gatacatcaa ttatctttat cactcatgac cttggtgtag tagcgaatgt
    19021 cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt gaagttggaa catcggaaga
    19081 aattttctac aacccgcaac atccctatac ttggggactg cttggttcaa tgccgacatt
    19141 agatagtgcg aacgatcgat tgtatgctat tcctggttct ccaccggatt tattgaaccc
    19201 gccaaaagga gacgcattct atccaagaaa tgaattcgcg atgaaaatcg atgcagaaca
    19261 agaaccacca ttttttgaac tatcaaaaac acacaaggca gcaacttggc tacttgctcc
    19321 gcaggcaccc aaagtgacac cgccagaaga aatccaacgt cgctgggcta tctttaaaga
    19381 gaaacaaaaa catacttacg gtcaaggagg aattgctgat gccaaataaa ctacttgaag
    19441 ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa tgaagtacat gctgtaaatg
    19501 acatcagctt tcacatttat gaaggagaaa cattcggttt agttggagaa tctggaagcg
    19561 gaaaatccac tactggccgt acgatcattc gcttgaatga acctactgat ggtgagatat
    19621 tattcgacgg tcaagatata acgaagatca aagataaaaa aggtttgacg aaattccggc
    19681 atgatgttca gatgatcttt caagatccat acgcttcgtt gaatcctcgt atgaaagtca
    19741 gagatatcat tgccgaagga attgatgtta atggactagc taaatcatct gaagagcgtg
    19801 caaaaaaagt cgatgatcta ttgagaactg ttggattgaa tccaagccac ggtactcgtt
    19861 atcctcatga attttcaggt gggcaacgcc agcgtattgg tattgcacga gcattggcag
    19921 taaaaccgcg ttttatcatt tgtgatgaac caatttctgc actagatgtg tcgatccaag
    19981 cccaagtggt caatttgttg caagatctcc aaaaagaaca tcaattgacc tatctattta
    20041 ttgcacatga tttgtcgatg gttaaacata ttagtgaccg aatcggtgta atgcataacg
    20101 gcttgttatt agagatggga acaagtgatg aaatctataa tcacggtgtt catccttata
    20161 cagaaagttt gttatctgct attcctctac cagatccaga ccatgagaga aaacgcagaa
    20221 gaatcaaata tcaacctgaa ccagatgacg gacaagtacg tcaacttcgt gaaatcgcac
    20281 cagaacattt tgtctatgca actgagcagg aagtcccata ttacgcaaaa aaactaaaac
    20341 gacaaaagga atctcttctg gttgctaact aggagggaca tagatgctaa aagcttttaa
    20401 attccgtatg tatccgacag aagaacaaaa acagcaattg attcgtactt ttggatgtgc
    20461 tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa agttggcaac aaacaggagt
    20521 agccaatttt tcgctcacac ccgctacatt aaaaaaagaa tacccattct taaaagaagt
    20581 agacagtctt gctttagcaa atgcacagct taatttggat cgggcttttc gcaattattt
    20641 caagggacgt gccagcttcc caaagcttaa aacgaaaaaa agtatgtggc aatcttatac
    20701 gaccaacaac caaacccgta cgatctatct ggaaaatggc tacttaaagt taccaaaaca
    20761 aaaagaacta atcaagataa atcagcatcg gccagttgaa ggatcgattc gctccgctac
    20821 gatttctgct cgatataatg aagaatttta tgtcgcacta ctttgtgatg tttcgccagt
    20881 aaaaaaagaa tctttagcta aatggatcgg gatcgcctat catccgaaga cattgatcca
    20941 aacatctcga cctctagaag taactttacc aaaatttcac cagacagaag aaaagctgca
    21001 acatgcacag cggaaattga atgtgaaagt ccgctccgct caccatagaa agatcaggtt
    21061 agatcaggca agcaattatc agaaacaaaa gagaaaagta atggatcttt atctgaaaca
    21121 aaagaaccag cgagaagatt atcttgaaca attatctggg aaattggtca aacaatatga
    21181 ctacttattt gtagaatctt ttccaaaaga agaggcacat gctgattttt ctattcacga
    21241 ctggcataaa ttaataacaa aattgcgtta taagtcccaa tggtataata aaaaatttct
    21301 tttgatcaat accgacggag cagaagaaag caattccgta agaaagagcc aagtagtaga
    21361 aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg aatgaaactg ataggagcgt
    21421 tagggttagc cttgttaata aaaagtctta atgacttttg tttctaggaa tttttctttt
    21481 gaagaaaagc tcactaatta tgacaaacca ataaaacaat agcagaaaaa gttcgtacga
    21541 gctttttctg ctattgttaa ttttttattc agaaagttta cgaatacatc aaaattctga
    21601 aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt ttcttgaatg attaggtctg
    21661 aatcttttgc tcacaacctt attcatggtg ccccggcttg agctgataga tttttgaagg
    21721 acggccgatt cctacaggac gttcaccgat ctcatcgaaa tattggagca tagctttctt
    21781 aaaattggaa tgatcgatcg ttttataatc gatccctaag aatttagcaa atacttttcg
    21841 agcttccgta atcgtgaaat ctttacctaa aacttgtaga acttgcggct cgtgttccat
    21901 tttattgact acacggttga atgctttcaa gatgatttga ctatggtcaa aagccaaagt
    21961 atctttgcca gaagactctc cagtgactaa atcaagcgag atagccactt ctccgctaga
    22021 taaatggatc ttgttactat gacgttctag tgtgaaccag cgtacttcct tggcatcatc
    22081 tccggcgatt agcggttctt ctccaataaa ggctaagtag ctgactgtga cgacccagcc
    22141 tcgcggatct cgattaggcg tactgaatgt atgcagttgc tcaatatttt cttttgaaat
    22201 aaccacacca gtttcttcct ttgtttctct taagacgctt tctccagttg actcatcctt
    22261 ttggacaaag ccaccaggca atgcccatga atttcgataa ggatgccctt ttctttggat
    22321 caacaaaact ttcagctgat cttcttcttt gttatagcac atcagtacga tatcgacagt
    22381 caaagagggc ttgtcatatt ctggacgttc ctgtttttga taccagttaa gaaattcttc
    22441 ttcacttgct ttatgttcat aatataattt ttcttcttct ttagaagcaa aacggttcat
    22501 ctgaccacct ccattgacag taaaagtact ttttacctta ttgtagcaga ctctgaaaaa
    22561 aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa taaatggaag
    22621 tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc gctcttcacg
    22681 aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca aaatccaagc
    22741 tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca tacgaatata
    22801 aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg taaaaaaaag
    22861 atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt tattatacca
    22921 cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa caaaaacccg
    22981 catttattca acctattcat ccttttaaag gatagattga acgtagatag aagtcgttta
    23041 tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg tatactagca
    23101 aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga aaaggaaact
    23161 ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg caactcgtat
    23221 actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt tgatgtgttt
    23281 tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga agaactaggt
    23341 gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt atggaaaacg
    23401 cttgatatca gttatgataa attcattcgc acgacagatg attaccataa agcagctgtt
    23461 caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga atatgaaggc
    23521 tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga agtgtacaaa
    23581 gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt agaattggta
    23641 aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt ggaatactat
    23701 gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat caacaacttt
    23761 atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg gggagtacca
    23821 gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc caattatatt
    23881 actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg gcctgcagat
    23941 gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc aatcatgctg
    24001 atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct attgatgaaa
    24061 gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct tgtggaacgt
    24121 tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg gtcagatggt
    24181 gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa tgatctaggg
    24241 aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact cgttcctgac
    24301 tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc taacgttgtc
    24361 ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc tgaaatctgg
    24421 gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt attagcaaaa
    24481 gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga atctttgcgt
    24541 atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat cttcaatcaa
    24601 ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga atttccttca
    24661 ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat ggaagaagaa
    24721 gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga tacagtcaaa
    24781 tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa atttgatgta
    24841 tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt agaaggggca
    24901 gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca aatcttgtca
    24961 ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt cgtcattgta
    25021 gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat cctttctgca
    25081 gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc aaatggctca
    25141 gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc cctttttccg
    25201 aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat catataatac
    25261 agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt cgctgatttt
    25321 tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca taatgataaa
    25381 gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct tacattcata
    25441 aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag cgttgatgtg
    25501 ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat gtgtgattga
    25561 aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc tttattttgt
    25621 aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa cggcttggta
    25681 agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc gtacgtccga
    25741 taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca
    25801 aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt gccatcactc
    25861 caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc tttgtaaatg
    25921 ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc
    25981 caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg atcaatgcaa
    26041 cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca acagtcatta
    26101 atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct ttacgggaaa
    26161 aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta aaatccataa
    26221 actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa agaaatacat
    26281 ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa attaaaatat
    26341 tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg
    26401 caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg gaaccgtgat
    26461 cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt ctacaagttt
    26521 gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat tgacagtggc
    26581 tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg aagctgcgta
    26641 tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact ttcctgctaa
    26701 tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac aattatctac
    26761 cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt tgaatttgtt
    26821 agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat taataccaat
    26881 tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc agttatttcc
    26941 aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt tgttagcaac
    27001 gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc
    27061 ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag tcgtttatct
    27121 attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg gtaatttgaa
    27181 tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc tggtcactat
    27241 tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg ggatccgcgt
    27301 gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga caaagctttc
    27361 aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag cttgtattat
    27421 gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga tgtggctatt
    27481 cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg aattgccacg
    27541 tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg gtgctatttt
    27601 cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta taggtgttac
    27661 cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat
    27721 ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc tttttctatt
    27781 tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt gcttatgtgc
    27841 taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc catttaaatg
    27901 ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa atgggggtaa
    27961 cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg caattaagcc
    28021 atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct ggcagttata
    28081 cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt gtagcattag
    28141 gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa gataaagtat
    28201 ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt catactagag
    28261 aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat atcggtggaa
    28321 tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac ttagggatgc
    28381 atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa gaggctgcag
    28441 caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg gcaccagttc
    28501 cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa aagattgctg
    28561 aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat
    28621 tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt cgttgaagga
    28681 aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga aacaataggg
    28741 tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac tcgtggagtg
    28801 atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat tatggaagct
    28861 gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga
    28921 agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa aaaggtcgtg
    28981 acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct tttagactat
    29041 ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc
    29101 atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg tattacgccg
    29161 aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat
    29221 tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat ttaaaaaaag
    29281 tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca
    29341 aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat tgacggaaca
    29401 attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt tgatccctgt
    29461 attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag atattttaaa
    29521 gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt taaaagtcgt
    29581 agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg aatcagattt
    29641 ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta tctctgctga
    29701 accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata tggaagccag
    29761 tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag attcagcgat
    29821 tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa aagcattttt
    29881 ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata acttgcagca
    29941 ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg aaacagctgg
    30001 aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg cattaagcaa
    30061 tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg
    30121 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt
    30181 tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg ttgtgggcct
    30241 gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat gtttttgagt
    30301 tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac aaggagatgt
    30361 gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc ttatacttga
    30421 tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag ttattagtaa
    30481 gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag aaaaaatgag
    30541 aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg gggtcattat
    30601 cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg cgtctcatgt
    30661 gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg cgctaggtac
    30721 gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat tgtcacatct
    30781 tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa
    30841 tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga tcggtgtgat
    30901 tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta atatgcctga
    30961 aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga ttggtgggct
    31021 tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac tcagcaaata
    31081 ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg
    31141 gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc tcatctagta
    31201 atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg caccaacatc
    31261 ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt tctgtttctt
    31321 tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg cctggtttat
    31381 ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt gtgtaaagca
    31441 tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg gcttcaggtg
    31501 aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg aagataactt
    31561 cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga atacgaagct
    31621 taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag aagttcatgg
    31681 caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa acttctttcg
    31741 gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca gccatagtca
    31801 ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt
    31861 tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat aatggctcat
    31921 ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt
    31981 gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct aagatctctg
    32041 ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat ttcaagccaa
    32101 aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg aaaaccatgg
    32161 caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata tttagttctc
    32221 cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg
    32281 aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa tcagttacag
    32341 aataattttc tgcattgtca tattttttat atacgttttt cagagccatt tctaccataa
    32401 tacgttccac actttctttt ttagttttat taactattta aagtataaat gaaagcgtta
    32461 actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat gtgctagttt
    32521 taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa ataagtatgt
    32581 taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa
    32641 aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa catgaacttt
    32701 ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg atccatcggt
    32761 ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg gaggatcagt
    32821 tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa ccagatgtaa
    32881 cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat atcggaacag
    32941 ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga atcgtccatg
    33001 aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat aacgtttaat
    33061 gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata taatacgaac
    33121 gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat gtgaaaatat
    33181 gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat aaagcgttaa
    33241 gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc ggttttattt
    33301 tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa
    33361 caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt gatggtcact
    33421 ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac atttattcca
    33481 caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc agttttagct
    33541 gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt tacattgaat
    33601 gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat cacttggaaa
    33661 aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt gacaccatta
    33721 tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc gccccgcctg
    33781 ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt aggacgtagt
    33841 attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa aacaagcagt
    33901 cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt gatagagccg
    33961 tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga caatagctcc
    34021 ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat gggcaatcac
    34081 aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt tctctgtcaa
    34141 tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac tgatcatcgt
    34201 tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt gaccgccaat
    34261 cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag aggcttttag
    34321 ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat tttctcgcaa
    34381 tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac gtaatgtatc
    34441 acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg ttgtgtcata
    34501 aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc ctactatagc
    34561 aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc
    34621 ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg gttccaaggc
    34681 ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa atgctcgttc
    34741 tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga taaatggttt
    34801 agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc
    34861 actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa ggtgatctac
    34921 aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc ctttttcata
    34981 tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct gaaaagcttt
    35041 cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc caataatcct
    35101 ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca caaggaaaat
    35161 aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca tcataaaaat
    35221 aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg cagaaattgc
    35281 aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt gggagttttg
    35341 atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc ttaattttgc
    35401 aacggataag taagctaacc gattgcttaa cacttggatg agccattgac tgactacgtt
    35461 gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga agcggacctg
    35521 tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca caactgttgc
    35581 aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat aggcagtaag
    35641 gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca taactattct
    35701 tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg
    35761 gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc ttcttgaata
    35821 gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg tagcgcctta
    35881 cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa aatcagtaat
    35941 gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa
    36001 tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg gacaaattct
    36061 tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc tttcttaccc
    36121 cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg aacaagagca
    36181 atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc cattataact
    36241 gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt
    36301 ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt aatattttgg
    36361 agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc gataattgct
    36421 tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct
    36481 agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac gacgaggttt
    36541 gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc aagaacttgt
    36601 ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa acagatgatc
    36661 ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt ggaaacacgt
    36721 aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc tttttttgtc
    36781 tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg ttcattgagc
    36841 tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat gataaaaaat
    36901 aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc ccagtcgatc
    36961 acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa taaacgaatg
    37021 accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt gatcaaggta
    37081 tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt gattagctga
    37141 ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg gcaaatgata
    37201 gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc aatgattttt
    37261 cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa aggcaggaca
    37321 agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg cttacgatag
    37381 tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat ttgtccacaa
    37441 ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa agagaacaag
    37501 ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata gactattttt
    37561 tttaaaaaaa agaaaatgag aaaacatgcc agtctcttca ttcataggaa cgtctatttt
    37621 acttaatttt tctataaatg aattctgcat aaaaaatata tcaagtaaat gtttatagat
    37681 gaaactctat ttacttcttt tttaatttgt tcttgcaatc ttttttgcta cgtaattttt
    37741 gtaaaaaagt gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga
    37801 aaaatattta ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa
    37861 ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa
    37921 gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc
    37981 aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca
    38041 ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt
    38101 cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac
    38161 agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa
    38221 aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat
    38281 aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag
    38341 ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt
    38401 atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat
    38461 aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc
    38521 tgtctataat aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc
    38581 cgttttctat gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt
    38641 agagaaccag acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag
    38701 gttagctgat cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa
    38761 tgatgagcaa ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc
    38821 ttttgaaaaa caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa
    38881 agaggaacag ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga
    38941 cgccagtttg aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg
    39001 attaccactt ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt
    39061 ctatcagtat tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa
    39121 ttgtgcacaa tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa
    39181 gggagcgtac actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg
    39241 gatcttgttt ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac
    39301 ctatcttgat caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa
    39361 aacacgtctc ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat
    39421 tcgaagaata cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg
    39481 gctagaactg atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc
    39541 agtcagtgga ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga
    39601 gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc
    39661 tgaaatccat gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa
    39721 aaatacagca cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga
    39781 gaagcaacag cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt
    39841 caagaaaaat aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca
    39901 attatttgat tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta
    39961 ttggacacaa tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt
    40021 cgatggaaac agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg
    40081 gttaccggaa gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca
    40141 gtcttatcca tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt
    40201 agatattgaa gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa
    40261 atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc
    40321 tagcagtatg gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga
    40381 tatgccacta gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa
    40441 cagcgatgtt tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca
    40501 tcagtttttt aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc
    40561 tttagcaatc acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga
    40621 agagactgtt cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc
    40681 caaagcactc attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact
    40741 gctagaaaaa agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca
    40801 attaaaagat aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat
    40861 tggcaccaca gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc
    40921 gggggaaaag atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa
    40981 ggaaatattc aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt
    41041 gactattcta gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga
    41101 ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag
    41161 ctgtctcatt gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa
    41221 atggaagcag tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga
    41281 agagcattat agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag
    41341 tgaagataca gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca
    41401 ctaattttgg catgcaactt gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa
    41461 gtggatagag aaattatctt agcttcccat ggaaaatttg cttcagggat attagattca
    41521 ttagagctga tctatgggaa aaatcattct attactgtat tcgattgcta tactgatgag
    41581 aattttgatt tagcggaaac agtaaggcag ctttttatga ggtacaaaga taaagaaatc
    41641 attgttctga cggatctttt tggcggaagt gtcaacaacg aattcttaca atatatcaat
    41701 cgggatcatg tctatctcgt tgccggattg aatttgccac tattgattga gctagtatct
    41761 cggttggacg ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa
    41821 aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag
    41881 gaggaagaaa aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag
    41941 ctttttcatg gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac
    42001 ctacgaatga gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag
    42061 tgatcaaatc aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata
    42121 agttattcat cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag
    42181 aaatcaaaac catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa
    42241 aagcggtaaa tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa
    42301 tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt
    42361 aagaaaggaa gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc
    42421 gcacaatcag aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg
    42481 tttacaggaa tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag
    42541 ttagcattta tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt
    42601 gggattttag ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta
    42661 ctaggtttgc cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg
    42721 attccaatta tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc
    42781 gagcatatgc acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg
    42841 gtttcttata tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc
    42901 gttcaaaagg ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt
    42961 gctcgtttgc tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct
    43021 tcttacttag agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg
    43081 gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa
    43141 gaagatgaag aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt
    43201 attttggcgt tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc
    43261 ttatgtttat gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc
    43321 agcagctttg aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat
    43381 tttaggaatc acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac
    43441 tgcgatcgat agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt
    43501 cttttgggga actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg
    43561 aaatatttta ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg
    43621 ctatctagca acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc
    43681 aaacggtatg atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg
    43741 agcaatgact gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa
    43801 tgctgtgact gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc
    43861 atttggcata gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg
    43921 actagctttc tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga
    43981 aaatgtttac gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga
    44041 aaaagaatga tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag
    44101 caacgggttc atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg
    44161 atcttactgt cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt
    44221 caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag
    44281 taaaaatgat acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc
    44341 cacttgcttt agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct
    44401 tcgttacaaa aggctattct gcgactgttt tacagttact actcttaggg ataggaattg
    44461 gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca
    44521 aaataatcaa ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc
    44581 aaagtttatt tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa
    44641 cagctaaaga agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg
    44701 agttagaagc ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct
    44761 ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc
    44821 ggtacgttgg agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg
    44881 gattagcaat agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac
    44941 tattcgctta taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag
    45001 actttgatac agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg
    45061 aagaagaaca aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa
    45121 tcatcgataa actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa
    45181 cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct
    45241 ttgtacagca tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta
    45301 tcgttatttt gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa
    45361 tcaagaaagt aggagcgaca ctgattggat ttatcgataa agcgaacagt aagttgtctc
    45421 agctatgcga ttttgtggtc acttatccag ctcctggaac agaacaaatc aaatttttta
    45481 tggcagcaga tcgattgatg tatttgaatg gagaattcga agcttattca gaatactatg
    45541 aacagctcga acgttattta ccaacagggt tagtggaagc agagaaaaaa gcagatgcat
    45601 ttggcttatc atttgcagaa aaacatcgac gtgatagtat gcattatttt attggagcag
    45661 ggaatcaatg gggagctgtt tattcatatg ccatgtgtta ctgggaagaa caaagctggc
    45721 ttccatcgaa atctatccat gctgccgagt ttcttcacgg gacgttagag atcgtagaag
    45781 aaacaacccc agtcacatta tttttaggcg aagatgaaca gagagtgtta tctgaacgtg
    45841 tggctaaact tttgccaaaa atttgcagca attatacatt gatagacaca aaagactatc
    45901 cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg
    45961 tagtgacaca acgcatcgat gctcatgtgg agcagttgaa ctgccatcca ttagagatac
    46021 gccgctacta tcgtcagttt gattattagg atagactgga taaaaagaat ataaaaaata
    46081 aagagagcag cgataactgc tccctttatt tttttaaaat attggcatca attacgttat
    46141 aaaacaca
//
LOCUS       DACCCM010000012        45005 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCCM010000012 DACCCM010000000
VERSION     DACCCM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.012"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            374..1477
                     /locus_tag="IVK78_000711"
     CDS             374..1477
                     /locus_tag="IVK78_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6374205.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1761..2441
                     /locus_tag="IVK78_000712"
     CDS             1761..2441
                     /locus_tag="IVK78_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6374206.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2531..3565
                     /locus_tag="IVK78_000713"
     CDS             2531..3565
                     /locus_tag="IVK78_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6374207.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3579..3914
                     /locus_tag="IVK78_000714"
     CDS             3579..3914
                     /locus_tag="IVK78_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374208.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3898..6360
                     /locus_tag="IVK78_000715"
     CDS             3898..6360
                     /locus_tag="IVK78_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374209.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGIPLQFDVTANVAKMTENGDKYDTSPN
                     KILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGGNLNPGA
                     YAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLTMGLTEG
                     RFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQGGLLSP
                     VALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIHYRLYSK
                     GTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTNMYPSFA
                     YKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQENVTFT
                     LTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLSKIGDTI
                     HLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTILVPKQEL
                     TLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLTSLFKND
                     QGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLLEVNSST
                     NAQPGATYQGMVTWELTAGPTS"
     gene            6416..7975
                     /locus_tag="IVK78_000716"
     CDS             6416..7975
                     /locus_tag="IVK78_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6374210.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7972..9336
                     /locus_tag="IVK78_000717"
     CDS             7972..9336
                     /locus_tag="IVK78_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6374211.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9507..10577
                     /locus_tag="IVK78_000718"
     CDS             9507..10577
                     /locus_tag="IVK78_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6374212.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGLMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10592..11260
                     /locus_tag="IVK78_000719"
     CDS             10592..11260
                     /locus_tag="IVK78_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374213.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11280..11858
                     /locus_tag="IVK78_000720"
     CDS             11280..11858
                     /locus_tag="IVK78_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6374214.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12261..13172
                     /locus_tag="IVK78_000721"
     CDS             12261..13172
                     /locus_tag="IVK78_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6374215.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13428..14330)
                     /locus_tag="IVK78_000722"
     CDS             complement(13428..14330)
                     /locus_tag="IVK78_000722"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP6374216.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTKNISQLSVLGSDEVGNGSYFGPLT
                     VCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKYNEIQP
                     KYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQLQEKLY
                     FVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKLLEKGG
                     MPMLEKYAKLHFANTEKALKLLKK"
     gene            14532..14969
                     /gene="zapA"
                     /locus_tag="IVK78_000723"
     CDS             14532..14969
                     /gene="zapA"
                     /locus_tag="IVK78_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP6374217.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15027..15575
                     /locus_tag="IVK78_000724"
     CDS             15027..15575
                     /locus_tag="IVK78_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP6374218.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15690..18050
                     /locus_tag="IVK78_000725"
     CDS             15690..18050
                     /locus_tag="IVK78_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6374219.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18137..18451
                     /gene="trxA"
                     /locus_tag="IVK78_000726"
     CDS             18137..18451
                     /gene="trxA"
                     /locus_tag="IVK78_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP6374220.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18633..20411
                     /gene="uvrC"
                     /locus_tag="IVK78_000727"
     CDS             18633..20411
                     /gene="uvrC"
                     /locus_tag="IVK78_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP6374221.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20556..21455
                     /locus_tag="IVK78_000728"
     CDS             20556..21455
                     /locus_tag="IVK78_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374222.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21448..22710
                     /locus_tag="IVK78_000729"
     CDS             21448..22710
                     /locus_tag="IVK78_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374223.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22871..24769
                     /locus_tag="IVK78_000730"
     CDS             22871..24769
                     /locus_tag="IVK78_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP6374224.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25068..25787)
                     /locus_tag="IVK78_000731"
     CDS             complement(25068..25787)
                     /locus_tag="IVK78_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP6374225.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26124..26456
                     /locus_tag="IVK78_000732"
     CDS             26124..26456
                     /locus_tag="IVK78_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374226.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26443..28446
                     /locus_tag="IVK78_000733"
     CDS             26443..28446
                     /locus_tag="IVK78_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP6374227.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28465..28935
                     /locus_tag="IVK78_000734"
     CDS             28465..28935
                     /locus_tag="IVK78_000734"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP6374228.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28956..29540
                     /locus_tag="IVK78_000735"
     CDS             28956..29540
                     /locus_tag="IVK78_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP6374229.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29555..30541
                     /locus_tag="IVK78_000736"
     CDS             29555..30541
                     /locus_tag="IVK78_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP6374230.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30531..30842
                     /locus_tag="IVK78_000737"
     CDS             30531..30842
                     /locus_tag="IVK78_000737"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP6374231.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30864..32645
                     /locus_tag="IVK78_000738"
     CDS             30864..32645
                     /locus_tag="IVK78_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP6374232.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32638..34014
                     /locus_tag="IVK78_000739"
     CDS             32638..34014
                     /locus_tag="IVK78_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP6374233.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34021..34656
                     /locus_tag="IVK78_000740"
     CDS             34021..34656
                     /locus_tag="IVK78_000740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP6374234.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34686..36041
                     /locus_tag="IVK78_000741"
     CDS             34686..36041
                     /locus_tag="IVK78_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP6374235.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKFAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36233..37627)
                     /locus_tag="IVK78_000742"
     CDS             complement(36233..37627)
                     /locus_tag="IVK78_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6374236.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37719..38690
                     /locus_tag="IVK78_000743"
     CDS             37719..38690
                     /locus_tag="IVK78_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP6374237.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38687..39468
                     /locus_tag="IVK78_000744"
                     /pseudo
     CDS             38687..39468
                     /locus_tag="IVK78_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(39637..40008)
                     /locus_tag="IVK78_000745"
     CDS             complement(39637..40008)
                     /locus_tag="IVK78_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6374238.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            40142..40585
                     /locus_tag="IVK78_000746"
     CDS             40142..40585
                     /locus_tag="IVK78_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6374239.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40628..41437)
                     /gene="yidA"
                     /locus_tag="IVK78_000747"
     CDS             complement(40628..41437)
                     /gene="yidA"
                     /locus_tag="IVK78_000747"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP6374240.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41456..42823)
                     /locus_tag="IVK78_000748"
     CDS             complement(41456..42823)
                     /locus_tag="IVK78_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6374241.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42807..43637)
                     /locus_tag="IVK78_000749"
     CDS             complement(42807..43637)
                     /locus_tag="IVK78_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP6374242.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43800..44237
                     /locus_tag="IVK78_000750"
     CDS             43800..44237
                     /locus_tag="IVK78_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP6374243.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44313..44933
                     /locus_tag="IVK78_000751"
     CDS             44313..44933
                     /locus_tag="IVK78_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP6374244.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14746 a         7726 c         9567 g        12966 t
ORIGIN      
        1 gagctaatac cgagggtcag ctgattgaac accgttttat cgagaggttg tgaaaaagtc
       61 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
      121 tttgacttaa taccgagaaa atggcttttg aacaccgttt attcaaaaaa agggactttg
      181 acactacttt tgtcaaagtc ccatcggttt tttatctatt tttattacaa tcatttcttc
      241 cagagtagtg acatctggaa tggctgaaat gtaaacagat tatttgcctg tatcaatgtc
      301 gaagagtaat ataaaaagtg taaacgctat catattgttc atcattcaaa ttttatagag
      361 agaaggcatt gagatggaaa aaacatatca agcaggtaca aatgaaggaa tagtagattt
      421 tatcaatatg gaagatttag aaatcgcggc ttcacaagtg attccagctg gtggttatgg
      481 ttacatcagc agtggtgctg gcgacttatt tacttatcaa gaaaatgaac gggcattcaa
      541 tcatcgtttg attattcctc atgtattaag agatgtagaa ttacccgata caactacaca
      601 ttttgacgaa gaaatgctga ctgcaccaat catcatggca ccagttgctg ctcatggatt
      661 agctcatgtg aaggcggaga aagcttctgc aaaaggtgta gcagattttg ggacgatcta
      721 tacagcaagt tcttacgctt cttgtacttt agaagagata agagaagcag gcggggagaa
      781 agcaccgcaa tggtttcaat tttatatgag caaagataat ggaatcaatt tggatattct
      841 tgaagtggcc aagcgtaatg gagcaaaagc aatcgtattg acagcggatg caacagttgg
      901 cggaaaccgt gaaacagacc gcagaaacgg gtttaccttc ccgctgccaa tgccgatcgt
      961 tcaggcttat caatcaggag taggccaaac gatggatgct gtatataaat cttccaaaca
     1021 aaaacttagt ccaaaagatg tagaatttat cgctgctcat tctgatttgc cagtctacgt
     1081 gaaaggtgtt caatcagaag aagatgtcta tcgatcatta gaatctggtg ctggtggaat
     1141 ttgggtatct aaccatggtg gccgtcaatt agatgggggg cctgctgcgt ttgattcgtt
     1201 gcaatatgtg gcagaagcag tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg
     1261 acgtggacaa catgttttca aagcaatcgc atcaggtgcc gatcttgttg ctattggcag
     1321 acccgttatt tatggattgt ctttaggagg aagtacagga gtccatcaag tgtttgattt
     1381 ctttaaaaca gaattggaaa tggtcatgca attagctgga acacaaacag tagaagatat
     1441 caaaaaaata aaattgcgtg agaatcgttt tatttaagaa ataaatgacg aaaaaaatag
     1501 gagtgcggca gatgatgccg tactcctatt tttatagttc aaggatgtca ctaatagaat
     1561 aatttgaaac gaaaaaataa aattgaaata ttgtttcata cttaataaaa aaggaagaat
     1621 attcatgtaa acgattttat tgtttttttc tcttttatac aaaaagggtc ataatttcaa
     1681 gtaagcggat gaattcatga ggattttttg catactgtct ctactatact taggtcacta
     1741 agaaaaagga gtgatttatt atgaaaaaga acatgattgg gacattatta ctttcaacac
     1801 ttcttctagg cggcttggca acaccagctt ttgctgaggg tcaagcgact tcaaaaggag
     1861 atattacctt tactgaacca acaaataccg ttgagcctct taaccctact gatccttcaa
     1921 aaccagtaga gccagcagat ccggaaaatc cagcaactgg ccagacaggt tctttaacat
     1981 tggatgtagt tcctgaattg ccatttggta cacacgagat tgaaagcgga acaaaaacgt
     2041 atcaagtaga cgcttcaaaa aacgatacac cttatctcca agtatcagat cgtcgaggtg
     2101 taggtgcaga tggccaagca caagggtgga acgtgacagt ttcggtttca gattttgtaa
     2161 acggttcaca agttttgcaa ggcgctgaat tagattttgg aacatccact gttaaaagta
     2221 cgtcagataa cgaatccact ggaccaacta gtcaaactgt aactgggttg agtaaagcaa
     2281 gtgcagcgac accaatattt acagcagcaa aagatcaagg tctgggaaca tggctttctg
     2341 tttatgatcc agcaaatatc acactgaaag tgccgaaagc agcagctgga acattcacgg
     2401 ctgatttgac ttggaacctc gtggctggcc cagtagcata gagtatgaaa taaattcaac
     2461 aaactgcggt gaaaatacta taggtgaagg attttttacc gcagttgttg ttgaaaggat
     2521 gtggtggaca atgaatcaaa gtaaacgact gtttgtttcg tttttttcta ttttaagtct
     2581 atttttcatt tttccgtcaa tcagccaagc aaaggattca gcaggagact ttggaatcaa
     2641 accggtattt ccagagaacc aaatagataa agcaatcggt tattttgatt tattagtggc
     2701 accggaacaa aatcagacac tagaagtaat tatcagcaat tcttcagatg aagaacgaac
     2761 atttgaggtc tctgtcaatc cggcggtaac gagtgatggt ggaacgatcg attatagtca
     2821 aaaaaaccca acgttagatg agacattgcc gtttgatgtc cgtgacgttt tgctcattgc
     2881 gaaaaaggaa atcaatgtat cagcacatgc agaaacaact atacctatag aagtgaaaat
     2941 cccagcaaaa agctttaagg gacgtgtatt agcgggtatt catgtttcac ctaaagaaga
     3001 agctgaaaca gaaaatgcta cagaaggagc acaaatcaaa aatcgtattg cttataatct
     3061 cgcagtcgtt ttacaagaaa gtcaagagac gatagaaccg gatctgaaac ttttatcagg
     3121 agatttggac gaggtcaacg caaagccaac tgttcagctt cgtttccaaa atcctcagcc
     3181 taggattatc agcaatttga tatttacgag caagatcttt tatgaaaatc agctttatat
     3241 tgaaaatacg tctaatgaat ttttagttgc accaaattcg aattttcact tgaatcttga
     3301 tttagctggt gataaagcaa aagccggcga ttaccgcgca gaaatcatag caaaatcagg
     3361 cgacagtaat gaatggcgat ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa
     3421 agtaaatgaa aattccgttt ttgcagtaca agaacaatct ttcccatggc tttatgcagg
     3481 attggttgct gcagttctag tgttatggtt gattttgctg tttttgtggg tccacaagaa
     3541 aaagaaaaaa ggagaaacaa catgatgagg tgatttcgtt gaaaacatcg atcttattgg
     3601 gagggattat tgcaagtttg ctgttcttca gctggcaaga gccagtccaa gcagatacca
     3661 aaagttcagg tacgattaca ttttatgatc taagtccagt tccgcatcca agtggttcag
     3721 tcaatggcgc atcaaaacag cagacacagc taccgattta taaagaatct aatcaacatg
     3781 gcgtattccc ggagacgaat gaagaaaaga tccgttttct ttatttgatc attggtatag
     3841 aattatggct tcttgcgtgg ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg
     3901 ttgctacaaa ataaattttc attttttcgc tccttctttt ttacttcgct ttttgtttta
     3961 agtatattta tcttttcacc ttcggcagaa gctgttggag caacgcctcc tgccatttct
     4021 tatgatccat acacgtatga aaatatcgta cctccaaatg gtggacgtag tgcagtcaat
     4081 ggcgggaagc ttgtccaact aactggggct aaccaattgc ctaaattgac tgctacttat
     4141 ttgaacgatg gttcaaatga tccttggaaa ggtggcacgg tccgttttca ttgggaaagc
     4201 catgtgaaac taacaaatgt acttgatttg gtgaatatca ctgcacaagg atttgcattt
     4261 gtggcgatca tcaatttgcc ggaacatgta tcaagcagtg atgtattaaa agcaattgac
     4321 tggaacaacg cctacttgta tattgccggt gcccccatca aggttactag cggtagttta
     4381 ttatcagcag gaagccatac cttgcgtttt gggatggggt cgtgggatac aaatactcta
     4441 ttggcagata ttattgctat tattacgcga ggtatcccgc ttcaatttga tgttacagct
     4501 aatgtcgcga agatgacaga aaacggagat aaatatgata cgagtccaaa caaaattttg
     4561 accaagggta aattacaacc ttctatgtct aaaaaggccg ttttttctgt tgatttttat
     4621 gattcgaata atattatcca agatcgtatg ttcaattcga ttttagctcc agctgcaggt
     4681 agattttatc ctaaattaac tggcggaaac ttgaatccag gagcatatgc attttctgca
     4741 agtgcagaga taaatacgtg gaatagttac atctcaacaa gtgattcaac aggaaaatat
     4801 aatacatttg ctgataccac agaatacgtg gatggaaata accagaacct tccaggctct
     4861 accaatagtc ggacattgac gatggggctg actgaaggac gttttgatca gttgccattt
     4921 agtcgctttg atcgcgtagt caactatttt acaggaaaaa acgttacaca aggagcaata
     4981 ctgactcata ctccagcaag cacaagtgga ttgaatcaga cagccaatat cgtgtacagt
     5041 gggaaagatg cgcaaggcgg attgctttct cctgttgctt taaaggtatt ggagcagtac
     5101 aagctagacg gaacggtttt accaacaaac ttaacagccg atagtcagtg gggaaagctc
     5161 ctttcttctg catcgtacca agtcacggcc aattggtcag caactgaact aaaaaagggt
     5221 tcgatccatt acaggcttta ttcgaaagga acccaaaaat tagcgacagg atggacagat
     5281 caacttttcc aaacgatcca atcaaataac gggggtgtaa atacagctac ttctacacta
     5341 cctgcattgg cttcagggca atattatttt gattatcgct tggtggacga tgtacttact
     5401 aacatgtatc cgtcttttgc gtacaaatgg cagtctgaac aagctggaat caccactctt
     5461 cctcaaataa cagtggctaa ttttccatct gtgagtgcag atagtcagct aaaaaacctt
     5521 agcagacaac ctgagacagg agatcccctg tctgctctat caggagatac gattcaagaa
     5581 aatgtgacat tcacgttaac aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc
     5641 tcacttcctg aaaatactac ttatatcaat ggttccttaa aattaaatgg tacaactatc
     5701 gcagataccg gcattcaaca aggagtttct gtcccagcag atctattgag caaaatagga
     5761 gataccattc atcttactta caactatcaa ttaaatactg tagatacttc tgtacaatcc
     5821 gtcagcatac tgacaaaggc agctgtactt tccagcaata ttacattagc agatggtgct
     5881 aagcttccta acccagttgt ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa
     5941 ttaacattag ttaatgtacc agatgatttt acatttggta acgatctccc caaaccgttg
     6001 aagacaagtt attatgaagc aaaaggcgat ttttcttttg atgtgcggga tactcggctt
     6061 ccaagcacgt ctccttggca actgacaggg acattgactt ctttatttaa gaatgatcaa
     6121 ggtcaagaat taagcggaac aaaattatac ttcaatcatt ctggatcaaa acaattgatt
     6181 cagcaaggtc aaaacacgtt gatttatgag agtgatggaa cagctaaagg agaggttctc
     6241 gttgactttc ccgataccga tggtttactc ttagaggtaa atagcagtac gaatgctcaa
     6301 cctggcgcaa cgtatcaagg catggtcaca tgggaactga cagctgggcc aaccagctaa
     6361 gtgattagaa tttgtatgga tctgtgataa ctgtcacaga tcctcttttt caaggatgag
     6421 aaggaaactt ctgctgttaa aatcagaaat gcttggagga gtggacttgg aacttttcag
     6481 tttgcttgaa aaaaatgaac aacggcaaat caaacttttc caacttttat tagaggaaaa
     6541 aggagagatt tccgttaata acgtacttac ccgcttatca gttgatcggg ctaccttgaa
     6601 agaagatctg ttttatttac aagctaactt ggaaaatatc aaacaattgg aaattgttat
     6661 ccaagaaggg aatattttat taatcagaca tggaaatata gcagcagtcg atgtttatta
     6721 tcattgctac ttgcgctatt ctacaaaata tcagatactt ctttatctgt tggaaaatgg
     6781 ctcatttgat cggcagaaac ttgtcatgga acttaatatc agttcggcta ctttgtctcg
     6841 cagagtgaag gaattaaatg attctttaaa tgagtttcat ctgaaaataa aaagcgggcg
     6901 gctgaccggt ccagaaagtc aaatttgtta tttttatttc caattatttt ggtttggacg
     6961 tccttatctt gaaaacaaaa aagaatacag tgatttatca ggaaacactt ttttagatct
     7021 gctgcaaaaa gagtttagtt ttactattac agaagaagga cgattgaaat ttgaaatatg
     7081 gctttccatt atgaagaagc gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa
     7141 tcttcctgtt ggattgtttc aaacgacaca atttttattg gacctccata catttttgac
     7201 ccgttacttc tttcaatatg cctttccttg gaatcaaaat gaaacgttgg ttttttatct
     7261 tttcatgatc agtaacttta cattagatac acataactcg catgtccagg agtttttgat
     7321 aagcgaaaaa tacaaagacg acgtagtagc tcaattgaat cgaacgttta aacaatcgat
     7381 agaagaatat ttctcttttt atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac
     7441 agtgatgcaa aatcacgctc attttgccta ttttagagga tggatgaata tttttggcca
     7501 agaaggatta aaacagcggt taactgaagt agaagataaa caattcctgg ttttatgtga
     7561 aagattggtc agacaaacat ttgctattgt tgattttact gaagtagacg aggaaaatgc
     7621 ccgctttgaa ttatttgctc gctatgcatc tattttgcgg acgctgatca agcagttaga
     7681 attacgtctg caaattgctt atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa
     7741 agaaatcatc caggaacaat tgctgaatac gatcaaatat gatttagtcg aatacgagaa
     7801 agaaaagcag tatgatatca ttttgactag ccaattgaaa gagtacccgt cacagaagtc
     7861 cgctaaagtt tttgtattca ctagcaacga atataattat gatttcccct atttgaacca
     7921 atttttaaaa gaatgttatt tagaaaaatt gaatttaagg atgaataaaa catgaaaatc
     7981 gtgatcattg gtgcttccca tgcaggcatc acagctgccc taaatctaag aaaacttcaa
     8041 ccgaaagcag aagttttact gatcgatgaa gatcataaag atggactagg ctatgtttca
     8101 aatggaatca acctttattt aaaaggaaag atacgtagtt tagctgaagt tgcccataac
     8161 atgcggactc ttcaagaaag tggggcaaaa ctgatcaccg aatggagagt aactgaactt
     8221 gatccagaca aacatcagct catcctcgca tccaaagaag gaaaaaacga gaccataacc
     8281 tatgataaat taatcgtagc aacaggttcc tctcctgtca ccttgtataa acaaattgaa
     8341 gctgaaaatg tctacaccta taaaaatcta gtgcaatcta agcaagtact tgccgcttta
     8401 aaagaagcaa aagaagtagt gatcttcggt gctggttata ttggattgga attagcagat
     8461 gctttgagaa ataaaggata cattattcat ctagttgatt atatgccgaa tgttttatca
     8521 agatatttcg ataaggatat gatcaacagt tttcaaaatc agctgcaaac taagcaaatc
     8581 aatttttatc caaatgaatt cttgatcgat tggaaaaaat cagaagagaa agtagtatcc
     8641 gtacaacttc tatctcaagc aataaaggca gatatggtca ttttttctgc ccaaactcgt
     8701 cctaatacga cattgttaaa agaaaaagtg gctttgtatg aggatgagac tgtgatagtc
     8761 aatgaatatc tgcagacaag tgatccggac atctatgcga ttggagatat cgtcccagtc
     8821 agttttgaca aaaataaacg gcatctcttt ttacctttag ttactcgagc tgtacatatg
     8881 gcaagagcag tagcgctaac tttgagtggt cagccaaccg cttacgacct cagacaaaag
     8941 ataacagcag cagtcattat agactacttt ttaggaactg tagggctgac agaagatgaa
     9001 gcgccattct tggaacaaaa taccggttcg tgttcaggag aatttgatct atttccacaa
     9061 tacgatgaag aaaacaaaac agtaaatgca aagctgatct accatccgga tacattagaa
     9121 ataattggcg gacagctgat aagccaagaa ttcttattat cagatttgaa cctgcttgcg
     9181 gatatcgtga aacacaaaac tacgattcca cagctagcag ttgaggattt tggatttctg
     9241 tctgaacaca cctctcgttt tcactacctc aatgagttag cttttaaagt actagctaag
     9301 agagcaaata gcaatagagt agataaaaga ctgtgagaaa tatcacggtc ttttattgtg
     9361 tggggatata ataaatatta atcaatcgag aataaaaaat tcatataatg aaatagcgga
     9421 agaatttttt gagcataata ttcgccaaaa atttatcaag tgataaaatt gttgtataaa
     9481 atgattattt caggaggagg agttaaatgt ttttagcatt aaatgaaatt atgcattcaa
     9541 agttacgtta tgcgttagtt gcaggggtga tgtttttgat cgcctacctg gtattttttt
     9601 tgactgggtt agcttatggc ttagctcaag ataatcgaac agcagtcgac aagtgggagg
     9661 cagattcgat tgtgctttcg aaagatgcaa attcaaatct agggatgtcc atgattacta
     9721 agaagatagc agaagaagtc gaaggtggga aagtggcgta tcttgcccag acacctggag
     9781 tcgtgactag taaagacagt acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg
     9841 ataaaaatca attcatcatg cccaatcttg ttgaaggaaa agcttttgat aacgatgatg
     9901 aggcagtagg cgatattagt ttgaaagaag aatatggtct agccgtagga gatacagtta
     9961 agttatctgg tagtgacaag acgtttaaac taactggctt tacggatcat gccaaattca
    10021 atgtctcacc agttctctat acaaccatca atgcttacca agagatccgc ttcgaaaaag
    10081 aagatacaag tgaaaatgca cggattaacg caatagttgt cagaggtaaa atcagtaatt
    10141 tacctgaaga tttagaacag atcaaaattt ctaaattcat caatgaatta cctggatata
    10201 atgcgcaagt attgactttt ggatttatga tcggattttt gatcgttatt gcggctattg
    10261 tgatcgggat tttcatttat gtactgacga tgcaaaagat taatatcttt ggtctaatga
    10321 aagcacaagg gatcacagga ggatttattg cgcgttctgt agtggcacaa acattcattt
    10381 tatcatttgt tggtattctt cttggtctat taggaacagt gggaacatcc cttgttctac
    10441 ctgatgcggt tccattccaa agcaattggc tgttttttgg cgtcattagc ttgttgatgc
    10501 tggtagtagc tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa atcgatccgt
    10561 tgaaggcaat tggataagaa ggaggaagag catgaaagca atagaattta aagaagtaga
    10621 aaaaagtttc caagatggcg accaagtagt taaagcattg aaagaaacaa ccatgagtat
    10681 cgataaaggg gaatttgttg caatcatcgg tccttctggt tcaggtaaaa gtactttttt
    10741 gactttggca ggcggattgc agacgcctag cagtggctct gtatttatca atgatcaaga
    10801 attcagtaat caaaaagaaa gtgaacgcgt taaactacgt tttaaagaaa ttggctttat
    10861 tttgcaagct tccaatctgg ttccttttct gacagtaaaa aagcagttat tattagtaga
    10921 taagatcaaa catgaacatc gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt
    10981 ggataagttg atcaacaagt atccagaaga actatctggt ggagaacggc aaagggtcgc
    11041 aatcgctcgt gcattgtaca atgatccgtc cattattcta gcagatgaac caactgctag
    11101 tttggattca gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat caaaagacaa
    11161 aaataaagca acgatcatgg ttactcacga tacacggctg attgacaaat gcgatagaat
    11221 atttatgatg gaagatggtg tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa
    11281 tggtccatcg atacaacaaa gatacacagc aaaaaatttt gaaagaagcg actgacttgt
    11341 ttatgtccaa gggatatctc ggcacttcaa caagagagat cgcacaaaaa gttgggatca
    11401 cccaacccaa tctctatcat tatttcggtg ataaagaaaa actatatacg gcagttttag
    11461 aaaaccactt aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg
    11521 gatttcaagt aaccttgaca aaaatggcgc agtttttgat tgagacacat ctggtcgatt
    11581 tgtttctgat gcttcacgat cttgaaagca atctttcaaa ggaaacaaga gatcatcttt
    11641 tctttctatg gaagaaaaat tatcgtgaac cttttgaaga aatcttttca gcaaaccaat
    11701 ctgtgctaag aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag
    11761 caccttacat cactcggtcg aaaagacctg tggaagaacc gctaaatgtg gcgcaattga
    11821 tcgatttata tttgaatggt gtattaggag aagcgtaaag ctgttatata gggagaaaaa
    11881 tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgttttt tttatatagg
    11941 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    12001 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    12061 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    12121 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    12181 ttttacagcc ccttttttgc gtacttaagc tgattttaag ttttgaccct tagtctggat
    12241 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    12301 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    12361 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    12421 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    12481 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    12541 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    12601 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    12661 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    12721 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    12781 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    12841 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    12901 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    12961 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    13021 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    13081 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    13141 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    13201 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt gaacaccgtt
    13261 taaggaagag gttgtgagcc tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa
    13321 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    13381 gtttattcgg aacaagggac tgtgacatga cttttgtcac agttccatta tttttttagc
    13441 agcttcaaag ctttttctgt attggcaaaa tgaagtttgg catatttttc aagcataggc
    13501 atgccgcctt tctccaatag ttttgccgct acttggtcag atttcgttcc agctcctgat
    13561 ggaagggtga ttccagcttc agctgatgct ttgtgtaact cttccaaaaa gctggcacga
    13621 ctgatgatag aagcagcagc aacagctaaa tgatattgtt ctcctttagt aacgaagtac
    13681 agtttttctt gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc
    13741 gtaaactgat cgatcaaaat cgcttctggt ttttctggct cgattttttt tagcagcaaa
    13801 tggatcgctt gattgtgcaa agcgactttc atccgaacgg cattgtattt tggttggatc
    13861 tcattgtatt tttctggaga taagacaagc agtttatagg ggatggtttt ttttaagatg
    13921 tcagcaagct ggatgatttg gctatctttt aactcttttg aatctctaac accaagttta
    13981 cgtaattctg caagctgatc acgtttcaca taagcagcac atactgttag cggaccaaaa
    14041 tagctgccat ttccgacctc atcactacct aaaacggata attgactgat attttttgtt
    14101 tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc tattgctgca
    14161 ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt cccgtctttt
    14221 tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg atagtatgct
    14281 ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat aaaattctcc
    14341 tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta agagattgcg
    14401 agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa aatatttgtg
    14461 gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc aaataggagg
    14521 aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc aaccaaacat
    14581 atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag ctgatcaatg
    14641 aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa gcagcaatct
    14701 tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc ttggagctgg
    14761 aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt gaaaatcgtg
    14821 tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa aatggtcaag
    14881 cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag aaacgaaaag
    14941 gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga aaaatttaaa
    15001 aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt ttattgctaa
    15061 ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc gcgattggat
    15121 atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt catttggaat
    15181 tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt gatcaggtat
    15241 ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt attttgctaa
    15301 ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg tatgtgccga
    15361 tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg gcttacgtta
    15421 ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc caaaatcaat
    15481 tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt accaacaaga
    15541 tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg aaaatatttc
    15601 gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg gcacagctct
    15661 tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa acattggaat
    15721 ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga aaagaagaac
    15781 tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt caagaaactg
    15841 aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa cttgaaaata
    15901 tccgtcccca tatgaaacgg atcgagatcg gagcagactt gaatggagtg gagcttgcac
    15961 aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat gatctttctg
    16021 acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc acgatccctg
    16081 ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact gatgacgctt
    16141 ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct gttcgtgaac
    16201 agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg atcatcacca
    16261 tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta tttggcggtg
    16321 tagtccacga tcaaagtgct tcaggacaga ctttatttat tgaaccaaaa caagtggtgg
    16381 atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt caacgtattt
    16441 taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac aatgcttatg
    16501 tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa gtaaaagcca
    16561 tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga catccactga
    16621 tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat caagccatcg
    16681 tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta ggtctgttac
    16741 aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa atcggtattt
    16801 ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt ctatcgacct
    16861 tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa aaaagtctgg
    16921 tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct ctagcaatcg
    16981 caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg cattatcctg
    17041 aattgaaagt ttatggatac aatcgggcaa atacgattaa tgccagcatg gagttcgatg
    17101 tggatacact aagtccaacg tatcgtttgc tgattggcgt tcctggacga agcaatgcgt
    17161 ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg aagcagttga
    17221 tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat cgccggaaaa
    17281 tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa gaacttcacg
    17341 atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa atggaaaaag
    17401 caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga aaaagcagaa aaaatcattg
    17461 ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag catcagttga
    17521 tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag aaaaataaag
    17581 tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg ttggttacga
    17641 cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa gtagaaatcg
    17701 gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct caaaaagagc
    17761 cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg ccgaatcaat
    17821 tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa tatttggatt
    17881 ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga acaggcgcac
    17941 ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc tttgagtttg
    18001 caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa agcttactaa
    18061 aggtaagcag atggttcgct atccaagtat agaaatgcta tactaatcat agattttaaa
    18121 aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg aagaaacaga
    18181 taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc gtatgcaagc
    18241 accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa ttgctaaaat
    18301 ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca ttccaacatt
    18361 aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt caaaagaaca
    18421 actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa gctgagggtg
    18481 ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta ttcaaattaa
    18541 tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga catgctataa
    18601 taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa aacaaattag
    18661 ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga acgatcattt
    18721 atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact ggcagccata
    18781 atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc gttacagaat
    18841 cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat cctaaataca
    18901 acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat gaaaaatatc
    18961 cccgtttggt gattactcgt aaagtactaa aagacaaagc actgtatttt ggaccatatc
    19021 ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt cctctgagaa
    19081 aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga caatgtctgt
    19141 gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat gaagtgaaaa
    19201 agtttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa atggaaaaag
    19261 ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc cgtgccattg
    19321 agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga gatgtatttg
    19381 gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt tttcgtaaga caaggaaagc
    19441 taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa gatttcttga
    19501 cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa gtcttgattc
    19561 ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg ctgcaaccaa
    19621 aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa gaatgcctca gttgctttga
    19681 atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca gttgaacgtt
    19741 taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat aactctaata
    19801 tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa ccggcaagaa
    19861 aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat gcatcaatga
    19921 gagaagtcat ctatcgcaga tattcacgag tgattcgcga agggttgcct ttacctgatt
    19981 tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc ttagaaaatc
    20041 aattaggaat cgatattcct attgctggca tggcaaaaaa cgataaacat aaaacaaatg
    20101 agttgctatt cggtatagat ttggctgtag taccgcttga gcgaaattca ccagagtttt
    20161 tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc catcggcagc
    20221 tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta ggaccaaaac
    20281 gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca gccacgacag
    20341 aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt gataaattac
    20401 atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca ttttatgtac
    20461 aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc tggtgtctaa
    20521 ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag gatcttgtta
    20581 aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc gatggaaaaa
    20641 tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt ttgatcttgg
    20701 attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt ggggagaaag
    20761 attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa gtaacgattc
    20821 aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag attgaaccaa
    20881 aaatcgattt ttggatggaa aaatttcagg taaaagggaa aaagacggat aaagtaaaat
    20941 ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc catgaaccta
    21001 aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct gaattgctaa
    21061 aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct agccacaata
    21121 tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga aaaatggttt
    21181 tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta ttcttggaat
    21241 ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg atccttcatg
    21301 aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa atctttactc
    21361 gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact ttggaagaga
    21421 ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac caaagatgtc
    21481 tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc ttttatcatg
    21541 ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt caataaaatc
    21601 ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa aacagatgat
    21661 tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc ggaagaaaaa
    21721 atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat gctttatagc
    21781 gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac tggcttgcaa
    21841 tcgatgcttc gagcaagtca attaggcatt tcaccagaag aagtagctag cttgagccaa
    21901 caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat gacagttgga
    21961 gaagataact cgacagtcca atatgtcgta agttatgtag caacgattgt cttgttcatt
    22021 attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa aggcacgcgg
    22081 atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg aaaactaaca
    22141 ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt tggtatagga
    22201 tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat ctcattggat
    22261 aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg cattttgatt
    22321 ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga tacagcaaaa
    22381 gcaattttgc cagtgactta tctatctcta ggcggatata tgcttggatt gatactagga
    22441 gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt cttatcttca
    22501 tatattatgc cggttcgctt agcaaatgag acagtcggta tgggtggagc attgatttcc
    22561 ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa catgtacaaa
    22621 tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt cattgaaaca atcgatttca
    22681 attatgagaa atgaacgaaa aaaagcataa aatatagaaa aatatagaag gaagctggac
    22741 atttattgtt cccagcttct tttttgtata gacctatttc tattttttta ttatattgga
    22801 tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac caatttttgg
    22861 aggcgtttta atggaccgct atttagattt actttcagag aagttcccta caacggaaga
    22921 tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag gaacagagtt
    22981 atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc gtatcggttc
    23041 aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg aaacggagat
    23101 cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg attggtacaa
    23161 aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt ttacgacagt
    23221 caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct atatcattga
    23281 ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa aaaaaatcat
    23341 tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg cgcagacgat
    23401 ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc gaggaactca
    23461 agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc agtgggggaa
    23521 tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat tgacgctttt
    23581 acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg gattaaattt
    23641 acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat ttacccctaa
    23701 aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc atcaagcact
    23761 ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg aatttaaaat
    23821 ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg ttttagaagg
    23881 tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg atcctaaaaa
    23941 attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc agcactcgtt
    24001 aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa
    24061 tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc tcttagcatt
    24121 tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa aacagataac
    24181 tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga tttggtatgc
    24241 gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt ttgagcgcta
    24301 ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc acttgcgaga
    24361 tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag aaggttcagc
    24421 aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga tcaaagcaaa
    24481 cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa caaccgggat
    24541 tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac attatccgtt
    24601 cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa aaaaagtgat
    24661 cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg aattacagca
    24721 acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag aaaaatgcta
    24781 gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg cagtctcctt
    24841 cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta tttctctaat
    24901 actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc ctgccaactc
    24961 ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa attgctcctt
    25021 gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta aaattcattc
    25081 acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag ttccggatgc
    25141 cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc aatgagtcca
    25201 tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc ctgatgatga
    25261 taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc cgaaacgatt
    25321 ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt tacggaccat
    25381 tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt caatagttga
    25441 agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa gatcaatgcc
    25501 tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg gtcttccatg
    25561 taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat tttctcaatt
    25621 agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc ttgagcatct
    25681 aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg gaaagtatct
    25741 gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt tattccccct
    25801 aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga cttgccccta
    25861 tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg aaattcatga
    25921 cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa taaaaaggtt
    25981 gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt attgaagggg
    26041 cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag aaagcatgta
    26101 cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa caaatccgaa
    26161 aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg aagacctatg
    26221 cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa cagctgaaac
    26281 agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa aaaatgaaat
    26341 tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa aagcatcaga
    26401 gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt cactaagatg
    26461 gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca agcagtacaa
    26521 ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa ccagtgggta
    26581 gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag cgctttaggc
    26641 aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg cgatagccgt
    26701 caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga agcaaattat
    26761 tccgaagaaa atttcatgaa taaattagaa gaggtccttg atttaaaaga aaagtgggaa
    26821 cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct tcattggcaa
    26881 cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga gatggggact
    26941 gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga tgacgtttat
    27001 atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa
    27061 tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga aacgtatcct
    27121 tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc cattgttcac
    27181 gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat ccaagaattt
    27241 gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa agaacggttt
    27301 atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga aaaacagaat
    27361 ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag tttcgaaaca
    27421 ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttatc
    27481 gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca
    27541 acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt
    27601 tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt tttaccccgc
    27661 gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat catctgggga
    27721 ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg tatccgcttg
    27781 cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt
    27841 ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa tatcaggagg
    27901 aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct gattgggatc
    27961 gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta tcttggcgcg
    28021 gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca atcgccgtct
    28081 aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg ttatatcggt
    28141 gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag tattggcgcg
    28201 gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt agggatcctt
    28261 ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc ctatgtccat
    28321 ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg cggacgtgcc
    28381 tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa aaaaaacaat
    28441 aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa tggtggaatg
    28501 gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg ttcagctaaa
    28561 ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc cgaaaaattc
    28621 ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg attcgttatc
    28681 gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg attgaacttt
    28741 ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc ccaagggaaa
    28801 gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac aaaaggaatc
    28861 atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc cttcctatta
    28921 gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa aaaatcatct
    28981 ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa gagcgaatga
    29041 agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat caaaaacaaa
    29101 aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat cgccaacaag
    29161 tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga ttgtttgcag
    29221 atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt atcaaaaatt
    29281 cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa aaatcagcag
    29341 cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt atgatggttt
    29401 taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa ggagttcagt
    29461 ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt tttgagattg
    29521 ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac taaatccctt
    29581 gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga tgattcaaac
    29641 aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc catatattta
    29701 tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc gtctatttcg
    29761 ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga tgcgctatac
    29821 tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc tggaccattt
    29881 gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc atacgcaagt
    29941 ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg attattttga
    30001 agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt tgacagagca
    30061 gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca ttgcttttat
    30121 tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa catatgggtt
    30181 catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt
    30241 agataaagat atggaagatt acacgaattt tctattagag acagattatg ctgattttct
    30301 gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt tgaaagatga
    30361 ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac tgccactact
    30421 agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg ttatcggcaa
    30481 aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct atgacgcata
    30541 agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt ggctttgatg
    30601 ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg
    30661 agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa acgatcgaac
    30721 gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa ggatcattag
    30781 ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat
    30841 agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc tccggacctc
    30901 ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta gtgggagatc
    30961 taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt caagtatatg
    31021 aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa gctttatctg
    31081 ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg ccattagata
    31141 cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg cccgcattgg
    31201 accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg
    31261 gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc atggtgccaa
    31321 atgggataaa aggtacgatc aaaacaatcg aaaaaggttc attcactatt gatgaaacag
    31381 tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa aaatggccag
    31441 taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg atcactggac
    31501 agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct gtacctggtc
    31561 cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca
    31621 tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat
    31681 tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg gtattgattg
    31741 ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg ggaatcacaa
    31801 ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac tccacttcac
    31861 gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct ggtgatgaag
    31921 gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct ggaagagtca
    31981 ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct gtatcgccgt
    32041 ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta aaagtattct
    32101 ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa
    32161 gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt caagctgact
    32221 gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa
    32281 tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca ttagaagtgg
    32341 caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc gatacattca
    32401 cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt gaagaaggac
    32461 gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc gtatcgattc
    32521 gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa ctagatcaaa
    32581 ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg
    32641 attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt
    32701 gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt
    32761 ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga aggaacaagt
    32821 ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga actaggtgta
    32881 tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga taacggacca
    32941 gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc agtagctcgg
    33001 gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt gaatacatta
    33061 gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa agaattagct
    33121 gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc cgtagtcttt
    33181 gctgctattg gaatcacttt tgaagaagca gaatttttca tggaagattt tcgtcaaacg
    33241 ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc aatcgagcgt
    33301 atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga aaagggaatg
    33361 catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg agagatttct
    33421 gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta cacgaactta
    33481 gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt tactcaaatt
    33541 ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt gactggttat
    33601 attacagaag gacagatcat cttgactaga gagctttaca agagtgggat tcagccacca
    33661 attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc cggaaagaca
    33721 agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca aggaaaacag
    33781 gcaaaagaat tggccgtagt tttaggtgaa tctgctttat ctgatgtaga caagatctat
    33841 gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt
    33901 tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc aagaaccgaa
    33961 ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa gtgagttctg
    34021 atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa aaaacagttg
    34081 acaactgcca caagagggca taaattatta aaagataagc aagatgaact gatgcgtcaa
    34141 ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca
    34201 gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggagccttt catcgaagaa
    34261 ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt
    34321 gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac acctttggaa
    34381 tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt tactaccctg
    34441 cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa
    34501 atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc tcaattagaa
    34561 gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg
    34621 ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatccttttt taaaagagga
    34681 aaacgatgat taaacaaagc aaacaaataa agaaacgctt gccggctgtt cagcttctcg
    34741 cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta ccatttttta
    34801 gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact tctgctgtct
    34861 gtgtgacggg attgacaaca ttgaatacag cagagcattg gaacagtgct ggtcaatttt
    34921 tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg attctatgct
    34981 ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa gaagcgctga
    35041 atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctgaaa tttgcctttg
    35101 tgatccaact gattggtacg gttgctttat cttttgtatt cgtgcctgaa tttggctggt
    35161 tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat gcagggtttg
    35221 atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc atggtggtat
    35281 ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta ctggaatatc
    35341 atcagaaaaa gaagataacg attcattcaa aaatcgcatt aaccgttaca ggtacccttt
    35401 tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggaaacg ctagtggaag
    35461 ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct agaacggctg
    35521 gatattattc agttgattac ctccatatga gccatgcagg tctgatttta acgatgtttt
    35581 tgatgtacat cggtggaaca tctgggtcga cggccggcgg attgaaaaca actacgttag
    35641 gtgtgttgct gatccagatg cgttcgatgc ttaaaggacg tacgagagcg gaagcatttg
    35701 gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt gttacgttga
    35761 cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct aaaacttctg
    35821 gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg
    35881 gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt atgtacattg
    35941 gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa agaacacaat
    36001 caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt ctgacagaaa
    36061 cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg gatactttaa
    36121 gtgcaaaatg ttcgtttatt ttttgaggca ctatccgttg ttgaacctcg ggtagtgcct
    36181 ctttcttatt gaaaggaatc aataagattt gattccttta tttatcaata ggttataatt
    36241 catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt tcaaggttat
    36301 tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta aagcgtctgg
    36361 aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta ggatcaatct
    36421 caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt tctactttga
    36481 aaagagaatc aaaaagctga ttggctcttt tcgtaagact taatttagtt tctaagatcc
    36541 tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat ccagaagttt
    36601 tcacatttat attaaaaatt gcttgttcta tcttgttttg gacatgtact ggaactacaa
    36661 gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag tagattgttt
    36721 ggttaggtaa taatttttgg aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa
    36781 atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact tcggctaatt
    36841 gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa ggcataagaa
    36901 agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt cgcattcctt
    36961 gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat tcaatgaaat
    37021 tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt gcagaaagtg
    37081 tgatagccaa tccattttga acaccggaaa caatagatag agattctaag gacggaatga
    37141 ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg cctttgggat
    37201 cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga ataaacggga
    37261 caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg atatgttcgg
    37321 ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata aatgtgccac
    37381 ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc ttgccaacgg
    37441 tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac tgagtgtttt
    37501 tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat cgataataag
    37561 gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca taagaattaa
    37621 taggcatttt ctacactcct taaaattgta taccatacaa ttttaccatt gattgtattg
    37681 attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt aattttgagg
    37741 aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg
    37801 attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta cctgacggaa
    37861 aagacaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga tattttggaa
    37921 caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct catttaggag
    37981 agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac gcaaatgcag
    38041 tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta gcaaatccgc
    38101 atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat ttgactgcga
    38161 tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa gaaagacgaa
    38221 atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata aatgcaatat
    38281 cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct agtaaagagg
    38341 catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg ggcacattta
    38401 ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc tatgtagatg
    38461 attactcagc taccttaaca gaaagcgggg acctgcttat agccatggca gaaggagatt
    38521 tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa aaaacatttt
    38581 ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt gaagatcttt
    38641 gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga acttagatta
    38701 ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag cggtttatgt
    38761 gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa acaatttatc
    38821 tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt ttacgccaga
    38881 tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt tatttaacta
    38941 ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc tatttgtgac
    39001 aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa aagtgccgat
    39061 attgactgaa tatgaagaag caattggggc taagattaaa gaagcctact tagcacgtga
    39121 tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag attttacagc
    39181 tatcaaaaat tttgaattag gaatagagtc atcgtgacag cagaaagtgt gcaaaaagac
    39241 tttgtttcta gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca
    39301 gcacattcta atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct
    39361 tatcaaatgt catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata
    39421 ataggaggaa aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat
    39481 gaattaaaaa taaattggtg gagagtggca gccttatagc tacaatggtt gttaaaaaaa
    39541 gacgcaaact ttttcaattt aatgaaaagt ttgcgtcttt ttttaattat tggtgtttgt
    39601 tttttattca atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt
    39661 ataaccatca gggtcaatga caaagtagta agaaggcgta gtgccaggta atccttttaa
    39721 gtcagtaatc tcaagacctg cagctttatg ttgctcgtgt aatttttcta aatcatcagc
    39781 tgcaatagcg atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta
    39841 agtcaactct aattcatagt catctccagg aagtgtcaaa taaacaagtg taaatttgtt
    39901 ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat
    39961 tgatgcttca agatctttca cccgaacaca tgtgtgagcc attttcataa attcatctct
    40021 ccttttcctt ttactcaatc ataacaaaaa gaagtaagaa aatagttgat ttgtgcttgt
    40081 tcatgagaaa tttctcatgt aaaatggata gaaaataata ctgttaaggt ggaaacaatt
    40141 catggataaa ccaatttttg gtgaaaaaaa gcaatctgct aattatcaaa cccgttatgc
    40201 agcatatatt gtcatagaaa gagacaagcg gattgcggtg atcgaggctc caaatggtgc
    40261 ttttttcctt cctggtgggg aaatcgaagg agaagagacg aaagaagaag cgattgtccg
    40321 tgagctgata gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga
    40381 tgagtatttt tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt
    40441 tgctgattct tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt
    40501 gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag
    40561 atggatcgaa aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt
    40621 ttatttatta cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg
    40681 aagtgatgac atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta
    40741 gaccagcata ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat
    40801 cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt
    40861 tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag
    40921 tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt
    40981 tgacgatgtt catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga
    41041 ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt
    41101 tgtcatcgat cgaatggaga tgactgccga cacggcgtgc catcacttcg atctctagga
    41161 aatcttcatg agtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga
    41221 gtgccccgtt ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact
    41281 gttcctggac acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa
    41341 cagcagcggc aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag
    41401 taccatcgat gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat
    41461 ttatttcaat caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa
    41521 ataaatcata atgatcacgc aaggagggat caaccatttc ttttggaaag aaaaatcgct
    41581 catctccttg ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta
    41641 tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc
    41701 gataaaaatc ataaggcaga tcgtagctag aattcacagc agtgtaataa acagggttat
    41761 agccaacctt ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag
    41821 aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa
    41881 taggatcagc agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt
    41941 tgaggtattc ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga
    42001 gttcaaaact gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca
    42061 aaatcacttc cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc
    42121 ggctaacgat ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt
    42181 cataaggacg aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag
    42241 tgaaataagc atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca
    42301 tttggacaac ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa
    42361 aaccttcttc tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct
    42421 ctacagtgat ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac
    42481 catgaccgac atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc
    42541 agcctccgtt gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc
    42601 ttgaaatctc ataaacgcct aatgaatggg taaaacggct atgttctgct ccgtgaaaag
    42661 tgaaagaaga agtgcctaat tgtttaatgc gtcgtaaacg ttggacttct ttcgaattga
    42721 tcaaatctaa gattacttta tgctggacat ggatatagtt atggactgga tcacgaaaaa
    42781 ctttttccat aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg
    42841 ttattacgtt gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt
    42901 tttagccagt cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt
    42961 gcaaatgctt ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt
    43021 gccattgtag caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca
    43081 gattgataaa atcgcttcat gacttgcccg cgtttttcct gattaccgtt gacacttaag
    43141 tacatcataa cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg
    43201 ccatggatgc ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt
    43261 atcggtgcct ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct
    43321 atggttattt tttttgtgtc actttgaggg aaaatcaaag aaatatttaa aattccttca
    43381 tctgatacga tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatctttc
    43441 agtgtttgga tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact
    43501 gtgcagtcat attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt
    43561 aatatatctg ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt
    43621 cgaatcgcag ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta
    43681 tactctaaaa tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg
    43741 ttgacaaaac aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa
    43801 tggaattatc acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag
    43861 gggaagtgca agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc
    43921 tgtacatacg ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca
    43981 aattagttcc agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga
    44041 aatttggcta taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga
    44101 ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg
    44161 ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct
    44221 tggattttac agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga
    44281 taaggagtag actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa
    44341 acaaaagcga attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg
    44401 tcatggattt ttctgattta gctaaccaga tccaaaacta tcttgagaaa tcagacagtg
    44461 aaattcgtga ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct
    44521 cattagggga taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag
    44581 aagtaaacca tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga
    44641 aagtcaacgc ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag
    44701 aagatacaga tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag
    44761 atgaagaaat cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag
    44821 atgatgaaga agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg
    44881 atgatgatga agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat
    44941 tttcttacga gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg
    45001 atttt
//
LOCUS       DACCCM010000013        44431 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCCM010000013 DACCCM010000000
VERSION     DACCCM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.013"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(72..542)
                     /locus_tag="IVK78_000752"
     CDS             complement(72..542)
                     /locus_tag="IVK78_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP6374245.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IVK78_000753"
     CDS             872..2512
                     /locus_tag="IVK78_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP6374246.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IVK78_000754"
     CDS             2666..3988
                     /locus_tag="IVK78_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6374247.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IVK78_000755"
     CDS             complement(4056..4490)
                     /locus_tag="IVK78_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6374248.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5391)
                     /locus_tag="IVK78_000756"
     CDS             complement(4555..5391)
                     /locus_tag="IVK78_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP6374249.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6133..6759)
                     /locus_tag="IVK78_000757"
     CDS             complement(6133..6759)
                     /locus_tag="IVK78_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374250.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7054..7389)
                     /locus_tag="IVK78_000758"
     CDS             complement(7054..7389)
                     /locus_tag="IVK78_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6374251.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7376..7660)
                     /locus_tag="IVK78_000759"
     CDS             complement(7376..7660)
                     /locus_tag="IVK78_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP6374252.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7716..9239)
                     /locus_tag="IVK78_000760"
     CDS             complement(7716..9239)
                     /locus_tag="IVK78_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6374253.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9232..10407)
                     /locus_tag="IVK78_000761"
     CDS             complement(9232..10407)
                     /locus_tag="IVK78_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6374254.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10411..10596)
                     /locus_tag="IVK78_000762"
     CDS             complement(10411..10596)
                     /locus_tag="IVK78_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374255.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10562..12256)
                     /locus_tag="IVK78_000763"
     CDS             complement(10562..12256)
                     /locus_tag="IVK78_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6374256.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12253..12726)
                     /locus_tag="IVK78_000764"
     CDS             complement(12253..12726)
                     /locus_tag="IVK78_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP6374257.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12795..12950)
                     /locus_tag="IVK78_000765"
     CDS             complement(12795..12950)
                     /locus_tag="IVK78_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP6374258.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13084..13464)
                     /locus_tag="IVK78_000766"
     CDS             complement(13084..13464)
                     /locus_tag="IVK78_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6374259.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13468..13683)
                     /locus_tag="IVK78_000767"
     CDS             complement(13468..13683)
                     /locus_tag="IVK78_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374260.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13686..14093)
                     /locus_tag="IVK78_000768"
     CDS             complement(13686..14093)
                     /locus_tag="IVK78_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP6374261.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14359..15834)
                     /locus_tag="IVK78_000769"
     CDS             complement(14359..15834)
                     /locus_tag="IVK78_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP6374262.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15824..16672)
                     /locus_tag="IVK78_000770"
     CDS             complement(15824..16672)
                     /locus_tag="IVK78_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP6374263.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16709..17071)
                     /locus_tag="IVK78_000771"
     CDS             complement(16709..17071)
                     /locus_tag="IVK78_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374264.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17371..17995)
                     /locus_tag="IVK78_000772"
                     /pseudo
     CDS             complement(17371..17995)
                     /locus_tag="IVK78_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(17992..18303)
                     /locus_tag="IVK78_000773"
     CDS             complement(17992..18303)
                     /locus_tag="IVK78_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374265.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18347..18640)
                     /locus_tag="IVK78_000774"
     CDS             complement(18347..18640)
                     /locus_tag="IVK78_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374266.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18834..19481
                     /locus_tag="IVK78_000775"
     CDS             18834..19481
                     /locus_tag="IVK78_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6374267.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19542..20687
                     /locus_tag="IVK78_000776"
     CDS             19542..20687
                     /locus_tag="IVK78_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6374268.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20768..21073)
                     /locus_tag="IVK78_000777"
     CDS             complement(20768..21073)
                     /locus_tag="IVK78_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP6374269.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21098..21529)
                     /gene="ruvX"
                     /locus_tag="IVK78_000778"
     CDS             complement(21098..21529)
                     /gene="ruvX"
                     /locus_tag="IVK78_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP6374270.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21526..21792)
                     /locus_tag="IVK78_000779"
     CDS             complement(21526..21792)
                     /locus_tag="IVK78_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP6374271.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22047..22772)
                     /locus_tag="IVK78_000780"
     CDS             complement(22047..22772)
                     /locus_tag="IVK78_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP6374272.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23133..23203)
                     /locus_tag="IVK78_000781"
     tRNA            complement(23133..23203)
                     /locus_tag="IVK78_000781"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23169..23171),aa:Gly,seq:ccc)
     gene            23320..24072
                     /locus_tag="IVK78_000782"
     CDS             23320..24072
                     /locus_tag="IVK78_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP6374273.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24088..24567
                     /locus_tag="IVK78_000783"
     CDS             24088..24567
                     /locus_tag="IVK78_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP6374274.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24818..26398)
                     /locus_tag="IVK78_000784"
     CDS             complement(24818..26398)
                     /locus_tag="IVK78_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP6374275.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26391..27134)
                     /locus_tag="IVK78_000785"
     CDS             complement(26391..27134)
                     /locus_tag="IVK78_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374276.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27310..28086)
                     /locus_tag="IVK78_000786"
     CDS             complement(27310..28086)
                     /locus_tag="IVK78_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6374277.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(28334..29170)
                     /locus_tag="IVK78_000787"
     CDS             complement(28334..29170)
                     /locus_tag="IVK78_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP6374278.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(29181..30110)
                     /locus_tag="IVK78_000788"
     CDS             complement(29181..30110)
                     /locus_tag="IVK78_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP6374279.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            30302..30655
                     /locus_tag="IVK78_000789"
     CDS             30302..30655
                     /locus_tag="IVK78_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6374280.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30729..31385)
                     /locus_tag="IVK78_000790"
     CDS             complement(30729..31385)
                     /locus_tag="IVK78_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6374281.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            31611..33545
                     /locus_tag="IVK78_000791"
     CDS             31611..33545
                     /locus_tag="IVK78_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6374282.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(33631..34854)
                     /locus_tag="IVK78_000792"
     CDS             complement(33631..34854)
                     /locus_tag="IVK78_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP6374283.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(34848..36746)
                     /locus_tag="IVK78_000793"
     CDS             complement(34848..36746)
                     /locus_tag="IVK78_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP6374284.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(36740..37858)
                     /locus_tag="IVK78_000794"
     CDS             complement(36740..37858)
                     /locus_tag="IVK78_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6374285.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(37941..38774)
                     /locus_tag="IVK78_000795"
     CDS             complement(37941..38774)
                     /locus_tag="IVK78_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6374286.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38767..39684)
                     /locus_tag="IVK78_000796"
     CDS             complement(38767..39684)
                     /locus_tag="IVK78_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6374287.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(39911..41035)
                     /locus_tag="IVK78_000797"
     CDS             complement(39911..41035)
                     /locus_tag="IVK78_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6374288.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            41218..42126
                     /locus_tag="IVK78_000798"
     CDS             41218..42126
                     /locus_tag="IVK78_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6374289.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            42267..43880
                     /locus_tag="IVK78_000799"
     CDS             42267..43880
                     /locus_tag="IVK78_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP6374290.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13429 a         8855 c         7405 g        14742 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     4981 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5041 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5101 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5161 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5221 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5281 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5341 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5401 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5461 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5521 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5581 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5641 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca
     5701 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt
     5761 ttaaggaaga gactgtgaag tcaacgtttt gacctgatta ttagagaaac aaatggcaaa
     5821 atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc
     5881 gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt
     5941 attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa cccatatcaa
     6001 agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt tatctatcac
     6061 tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa cttcaataag
     6121 ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag ctacataatt
     6181 tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt cccaagataa
     6241 cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta ttactttctt
     6301 atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat tttccaaatt
     6361 tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt tccaccaatc
     6421 tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg ttaaagaatt
     6481 cataacccta acaccattat ccaaacacat taattcgcta gatttaataa cagctttaaa
     6541 atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga aaataatctc
     6601 acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg ttttgaaatt
     6661 attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta aatgtagata
     6721 atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc ttttgaatta
     6781 taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa tcagcttgcc
     6841 aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat catctatcat
     6901 ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac aatagatttc
     6961 gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc ttaattatac
     7021 taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag ctatgatcgt
     7081 tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac tcttgttttt
     7141 ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa taataaacgt
     7201 caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca tttgtgaacg
     7261 cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc catcttcatc
     7321 ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta cattcttaat
     7381 gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc
     7441 gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat gacaccgcca
     7501 aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca atagcacttt
     7561 ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca atagtgtgat
     7621 cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc ctaatgaaaa
     7681 aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt tacaactggc
     7741 gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt gcgaacgatc
     7801 actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac ttgatttcta
     7861 cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt tccagcttta
     7921 ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa tgatttacca
     7981 ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt caatgtatct
     8041 aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg gtctaatgcc
     8101 acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt gaatgttttt
     8161 aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa ctgatctttg
     8221 acctcttgta caatattcac tgctgaatct tccacaacct catttgataa ggcaatctta
     8281 ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc aatatcacca
     8341 atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc tactggatat
     8401 ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt ataatttgaa
     8461 cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc tgcatttaca
     8521 gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata cccacgcaat
     8581 tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt ttgtgcttta
     8641 gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat aaacgattcg
     8701 taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt cacgacacta
     8761 atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc ttcttttttg
     8821 tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac cccttttgag
     8881 atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat
     8941 cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag tggctttgaa
     9001 taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa tgcttcgggt
     9061 gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt caacgcatag
     9121 cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac tttgggagaa
     9181 tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc attattcaac
     9241 tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta aatacgtaaa
     9301 gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca ttttcgaccg
     9361 cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt ttacttttgt
     9421 ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt caagtttcgt
     9481 atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga tatactggag
     9541 attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc
     9601 aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct tcaaaacatc
     9661 ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg ttctaagagc
     9721 attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg cgttctttgc
     9781 tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga caccacgagt
     9841 aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc
     9901 ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc
     9961 gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac cattatctaa
    10021 ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct tttcaccgtt
    10081 gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca aagcaaactt
    10141 taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca ccagttcatt
    10201 atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc
    10261 aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc ttgtaaagct
    10321 ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat ctcccgttgt
    10381 attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg tatttagcat
    10441 gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga catttgttag
    10501 taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg tatgaatgta
    10561 attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct ccttccgttg
    10621 tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt ggatcaattt
    10681 tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt
    10741 tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct tcataaactt
    10801 gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt acttctaaca
    10861 ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac gggtcataac
    10921 aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac tgaaatactt
    10981 ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca gctttttcta
    11041 gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat ttggtggcta
    11101 caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc catgaaacgc
    11161 tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg cctgtaatat
    11221 cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg tcctcactcg
    11281 cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt tgtttcaaag
    11341 caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct tcactttcaa
    11401 aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga tcgtccaatt
    11461 cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca
    11521 ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta ataacacaca
    11581 ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt ttacgagttt
    11641 ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct agcgtgctgg
    11701 tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc ttagagttca
    11761 taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta agtgaattag
    11821 acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc
    11881 ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca cttgcgatat
    11941 acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc ctgtaattcc
    12001 cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat cctaacatct
    12061 tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct ttggtggctt
    12121 gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa tcgcttatat
    12181 gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat aggactttct
    12241 caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt catcattttt
    12301 aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg aggtggcttg
    12361 ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg ctttctttac
    12421 tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg ctgttttcat
    12481 tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac tcaatggata
    12541 atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg ctgccatcca
    12601 gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc tagcgtccac
    12661 acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata ttttagggtg
    12721 tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta agctcacatt
    12781 ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat gatttttccg
    12841 ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct
    12901 gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat tatatacttt
    12961 ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct tttcacaacg
    13021 gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact atgccccctt
    13081 aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg aattatggtg
    13141 tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat catcttttag
    13201 ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta ggcaatcctc
    13261 acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc tactttgata
    13321 gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg ccgtttgctt
    13381 tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac atccagcatg
    13441 attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc
    13501 atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca agcacgttcc
    13561 gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt ctacatcttc
    13621 ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac tttccatatg
    13681 cataattatt ctacctccaa taaattagtt atcaattcat catctgttag tagcaaagcc
    13741 aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt
    13801 ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc acctgtatca
    13861 tcatagttgc cactatctac caatgatccc actactagcg attgacacca cgctactgac
    13921 tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc aaatgtggaa
    13981 taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa ccgatccatt
    14041 tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag catttacttt
    14101 cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta ccactcaatc
    14161 ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat ccagtaacat
    14221 tattccccag catattagct aactatttca aaagttttaa ggaaataagg tgttaccccg
    14281 ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg ctaggtgtta
    14341 ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat tttcataggc
    14401 atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct tccgttccca
    14461 accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg atcgaccatt
    14521 caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg tgagtacgct
    14581 atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct gtaaattttt
    14641 ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc acgctggagc
    14701 ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt ctttaattgg
    14761 attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt ctttatcttc
    14821 atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt cagctaacac
    14881 ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat tcttacaagg
    14941 caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat cattcgacgt
    15001 tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag gaggtctaat
    15061 tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt tgttcattga
    15121 agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat catctttctt
    15181 ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac
    15241 ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa actttattcc
    15301 tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt ctcgtgtgta
    15361 aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag gctgttgatc
    15421 ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc tagccacatt
    15481 tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt cttcaatata
    15541 tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat tgatccaagg
    15601 agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg ttccagctaa
    15661 attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt taatctgatc
    15721 gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat tgtcttcacg
    15781 ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag ccatgagctt
    15841 tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct ttatctttta
    15901 gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt tgattatcta
    15961 caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta ctaccttcgc
    16021 cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct acgggtcggt
    16081 attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa tcgggagctg
    16141 gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca acacttcttg
    16201 aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc ccgcttaaac
    16261 ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct tgttggataa
    16321 ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg atttccgtat
    16381 aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca acgccgtttc
    16441 gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca agcattaacc
    16501 caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa tcgttagttg
    16561 catcattcca accactagag ccttttattg gaattttagt tccaggagat aagggataaa
    16621 ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc aataaacctc
    16681 tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc ttcaactgct
    16741 caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga ttctgatact
    16801 ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg tttaaccatg
    16861 cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg tgttctgcat
    16921 gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca aagtaggttt
    16981 tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat tcaaggctat
    17041 cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt cttaattgat
    17101 agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag atagctattt
    17161 ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct ataattaagt
    17221 ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac aaagaatatc
    17281 gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat ggataaggct
    17341 ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag cagaacgatt
    17401 ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag tcattaattc
    17461 tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca tatctttctt
    17521 ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta tcacattcgc
    17581 tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag tcattgcttc
    17641 tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg
    17701 gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt cttttttacg
    17761 gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca tcttgtgttc
    17821 tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa gagtgaatgg
    17881 ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact gcccaccatt
    17941 catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt ttcattcaac
    18001 gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat acaaaatttc
    18061 ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat cgttcaagcc
    18121 acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt ccatttgttc
    18181 gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta attctgccgc
    18241 tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt gtgtcgattc
    18301 cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat ttttggtatt
    18361 cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca actataatca
    18421 actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg ttcatatact
    18481 tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct aataatgaaa
    18541 gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata attttaaaag
    18601 ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc cctcgttagc
    18661 atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac atttctatac
    18721 ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta acatactttt
    18781 ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa tatatgaatg
    18841 gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga caaagaatta
    18901 gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa ttatccccac
    18961 cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc aataacacca
    19021 ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta aacggatctt
    19081 atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat
    19141 tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct aatgctatca
    19201 ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca aacgcaataa
    19261 aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca attgcttatc
    19321 tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat tcagaaaaaa
    19381 atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta aaagagttca
    19441 cagctcttgt tactgagcta aataaacact attcaaaata ataataaact gcctcctcca
    19501 accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca ttcaagcaat
    19561 atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg aacaaaatga
    19621 ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg
    19681 ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg aacaacgaaa
    19741 cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc cgagaagcca
    19801 ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat ggaaatcttc
    19861 gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg gctgaaacta
    19921 ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac gctatcaatc
    19981 ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga accaccacta
    20041 agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg tttgattacc
    20101 taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta cgctttctct
    20161 tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac attgattttc
    20221 aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc tataaaatca
    20281 gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga acaatttcta
    20341 tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt gtaactgatc
    20401 cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct atttatgcaa
    20461 gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat gtagcaagac
    20521 acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt caaactagat
    20581 tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca aaagaaacga
    20641 aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg gcatacaata
    20701 tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt tctcctagct
    20761 ttatagtcta ttcttcttct tccgccatga atgtattgaa tacttcttca atcatgtccc
    20821 attctgcgtc tgtttcgatt tgttctaact cgccttctgt tccatcttcg ttttcgatgt
    20881 atgcataagc ttgtaattct acatcttcat cttctgaaac accagcagga tataaaagaa
    20941 catagttacg tccaaattct tcttgtccgt caactgttag taggatttca tataatgttt
    21001 catttccttg gtcatccacc aatgtgatgt gttcgtgtcc ttcatggtcg tgggaatggt
    21061 tatgttctgt cataagatta cctcttttct tttattatta aaataattgc ttacctgagc
    21121 cgtctaaata attttgtaag atcatcacag ctgctaattt atcgatgact ttttttcgtt
    21181 tggctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca gtggttaatc
    21241 gttcatcttg ataatcaact ggcaaggaga atagttcttt gatcttttct ccgtatgaca
    21301 tggaagcttc tgcccttggt ccgatggaat tgttcatatt tttaggtaat ccaactacaa
    21361 accggtctac ttcatattgt gctactaatt ccttcaaccg atcaaagcca aactcttctt
    21421 ctgcttcatt gatccgaatg atctctactc cttgagctgt ccagccaaag gggtcactga
    21481 tagcaacacc tactgtctta gaaccgacat ccaagcccat tactctcata aattcactcc
    21541 atgatttgag aggtagtcct tgacaattac ttccatgatc tcatcgcgtt cgtgacggcg
    21601 aatcaaattt cgagcatcac gataacgagg aatgtaggcc gggtctccag aaagcaagta
    21661 gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc
    21721 taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc
    21781 tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacaa
    21841 ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ccgaataaat
    21901 taagaaacac tatttgtatg tttttttcct tttttctgta gctttatcaa aagtactctt
    21961 ttcataaagt atacgctata tttctttgaa tgtatgtttt tacacaattt tttcataata
    22021 ttcataaaaa attcgaatag ctgctactaa ttttttgttt ttgttgcttg tcctgtctca
    22081 taattgatcg tgtagccatc ttgaacattg tagataaaga cattgaattc aatttttttg
    22141 tcttcaatgc tttctgcttc caattgcact cccctggcta ccagttcatt cccctcaaaa
    22201 tgaggagtga cacggtagcg tacatgatga tttgtttctt tgatatatgc cgcaatatca
    22261 ttttcgtgag caagcatgta tggagcattc aacgaacgtg tccctgtcat caagttttta
    22321 atgttgttat tttgaccagt cagctggaaa ccaattaaat ggctgcgatt atataaccat
    22381 tgcccgtcac ttaatttctt ttgtttccaa cctgtcgggt caatatacaa aggctctctc
    22441 ttttcagtag ggaacaaatc ttttccaagc atagcattgg cttgtcctac tctgttcaat
    22501 ttatcaatat ttgaatagct ttcccatgtt cctttggaaa ggctaagatc ctcttttgta
    22561 aattccggct gattcccatt gatttctatt tcttgatgtt ttccatcata ttctggcaca
    22621 tccgctactt gagaagcttg tggtgtggaa tcaaaaatag ttgtaatctg ctgaaagata
    22681 tctgaatctt tttgtgtggt ggaaacaccg aatgcccctg caatcacagc agcgacgatc
    22741 cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta acagctccct
    22801 aaatcttaac atatgttccc ctataaataa atactagaaa gaaatagaaa cagcaaaaaa
    22861 taaaacctgt tttaagatgg cttaaaaata caaaaaagag acggattcct cgtctctaat
    22921 cagtcaccac tctatctgta aggtatttat tattattcaa aaattttctt catactcaag
    22981 acaatacact gatttcacaa ccgcttggta aacggtgcgc aaaagcttgc atctgcggta
    23041 ataagtccat ccaagttaaa aacataagaa gttgtttttg cttggctgtt cttattactt
    23101 gttgcagagc agctttttca caacctattt taagcgggtg acgggaatcg aacccgcgac
    23161 atcagcttgg gaagctggga ttttaccact aaactacacc cgcaatacaa aaacagtata
    23221 cgcctctttt attccttcct caagtttttt tattctttac gcttctggta tgttttgtag
    23281 taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa agaattagat
    23341 ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa tgcggagatg
    23401 tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt ccgtttggtc
    23461 agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac catccgaatg
    23521 gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa tgatctttca
    23581 cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact gcaagcaatc
    23641 gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt
    23701 ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac tttagtcatt
    23761 ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat caaattttta
    23821 tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt acgttttggc
    23881 ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt tcctggagtt
    23941 caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg agtttctcgc
    24001 ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt tattttccag
    24061 cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt ttcttttagt
    24121 tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc attgatttgt
    24181 tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca aataggcgga
    24241 tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag cgatcttttc
    24301 tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt tcctctagtc
    24361 cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta tcgagaagcg
    24421 gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg tcttgtatta
    24481 tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg
    24541 aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag
    24601 acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt aaccgcagga
    24661 gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt ctcacatttt
    24721 tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat ctgtaaaaaa
    24781 ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg
    24841 ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg atcacaggaa
    24901 tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg ataagaataa
    24961 tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct gaacctctgt
    25021 taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct ctagcgaaat
    25081 aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta gggataaaaa
    25141 acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt aaaaaactct
    25201 gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag attggcaaag
    25261 aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata aaagaagtct
    25321 gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg attaacttat
    25381 tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata aaaatgattg
    25441 gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt actaattgag
    25501 cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt ctttttattt
    25561 tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc agctgtttta
    25621 cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat aaaccttgtt
    25681 gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc ggatgccgat
    25741 ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt ccagcaaatg
    25801 cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt tttttaaaca
    25861 ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga ataatcaaaa
    25921 atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga tcagctcgcc
    25981 atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa
    26041 ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt
    26101 gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga gttaaaccta
    26161 acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca aagttcatag
    26221 caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca
    26281 aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta ttcgtgagct
    26341 gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc ttatacatac
    26401 ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc tagcgtttca
    26461 tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc acctttcttc
    26521 aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt agaaaaaatg
    26581 actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa tgctgcttga
    26641 ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg cagcaacgtg
    26701 ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt ttcattttga
    26761 tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg
    26821 tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa
    26881 atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg attttccgtc
    26941 aatttcattc catctactaa aatcgttccc gatgatggtt caataatact aactaaactt
    27001 ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt
    27061 cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt acttacattg
    27121 atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg tacccttcgt
    27181 tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa cgactaggct
    27241 gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag atatgagtct
    27301 ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct ttagtttatc
    27361 aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta tttcgtttac
    27421 tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat ctttacataa
    27481 atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga gaatctcttc
    27541 atctttcaat actaggccta ttttattttt ataacctggc atgctcatta gataaaggat
    27601 ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc accttttttt
    27661 cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt ggatgctttt
    27721 tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa tctttcgatt
    27781 aataacagtt attgctttat tcggatgaga cgttagataa acaattttac acttttcatt
    27841 cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca aatcgatgtc
    27901 taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa caaattgtat
    27961 aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc tggattcttc
    28021 caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa atatacgcgt
    28081 taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat acaacttttc
    28141 aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac acctttgaaa
    28201 ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa atgaatacat
    28261 catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat
    28321 ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca gagactttct
    28381 cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa tagtctgatt
    28441 ggatggttcc ttctatttta taccattcat catttttaac cgttactcct tccggcatat
    28501 gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg aatcggaaca
    28561 agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca tagctgattt
    28621 ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct aaatagtttt
    28681 catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca ttcatttggt
    28741 ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat tgtgtctgca
    28801 tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg gcttctacga
    28861 tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc aaagctagca
    28921 gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt
    28981 gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact aaagccaaaa
    29041 gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg atatattgat
    29101 cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat aaaatcaaaa
    29161 accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct aagggcaatc
    29221 agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat cagtagattt
    29281 ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt gccgaacaga
    29341 cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat cgtaaaggcc
    29401 aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag cataattcta
    29461 gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa ataacgacct
    29521 gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg gatcagctga
    29581 ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg gtgattttct
    29641 gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata ggcaagccag
    29701 acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc catctgccaa
    29761 gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat cggagcagtc
    29821 agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg atgaacgatc
    29881 ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc agtaaaaata
    29941 gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa aaatacttgt
    30001 aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat aacgatcgat
    30061 aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat gagtttcctc
    30121 ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt
    30181 gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt gactttttct
    30241 gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag ggagaataaa
    30301 aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt catccgttgc
    30361 cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc cacgactaga
    30421 ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg aaacaccaga
    30481 agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc atcaaggaaa
    30541 agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc tactagatga
    30601 actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa aataatcgtt
    30661 gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct
    30721 ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg caagaaatag
    30781 atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc tgacggagca
    30841 ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt taactgattg
    30901 actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat ttgttggatc
    30961 ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac gatgcgccca
    31021 acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact ttgatggaat
    31081 ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac atttgtcaat
    31141 tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc ataaccatac
    31201 ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc actatctact
    31261 tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt atttaaaatc
    31321 cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat aatatgatct
    31381 ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca tcatagcagt
    31441 atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt ctttattcat
    31501 ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac tactaattga
    31561 aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc atgatattat
    31621 tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa aacatacatt
    31681 tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga
    31741 ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc gctaaaaata
    31801 aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat gaaacagttt
    31861 ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc
    31921 tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct tatttaaaag
    31981 aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat gaaaatgaaa
    32041 ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag aaaatcgata
    32101 ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg caacaccctg
    32161 acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt tcctggcttg
    32221 agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac cgttattttt
    32281 tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc cattacaaag
    32341 gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa tggaaggctt
    32401 atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg aaccttgttc
    32461 gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa atggaccggc
    32521 tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata gaaaagcctt
    32581 ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat gtcaccttat
    32641 ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta ggtccgaatg
    32701 gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt atccgtggaa
    32761 aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa gctgatctgc
    32821 atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact ccagaaaaag
    32881 agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa aaaccaatcc
    32941 ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg atgaacaaag
    33001 aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac aaagaagtat
    33061 tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat gaccgatatt
    33121 tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc aaagtctatc
    33181 taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc gccgaattgt
    33241 tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat tattaccaaa
    33301 gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag gttgaagaag
    33361 aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc gcgccagaaa
    33421 acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct gctagacaag
    33481 atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa gaatatgaaa
    33541 attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct ttttttattt
    33601 tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat tactagtcat
    33661 tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat ttaccccatt
    33721 atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt ccgttgtagc
    33781 atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata agttcttttc
    33841 tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca tgatcaccat
    33901 actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc aataacaaat
    33961 ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta aatcaaaata
    34021 tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc atgcttggcc
    34081 tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc ttggttttcc
    34141 atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta ctaaataatc
    34201 tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc cagtttcttc
    34261 actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc taccttgttg
    34321 tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc aaaaacgctg
    34381 aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc ccgttatttt
    34441 ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat tagtgataat
    34501 ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt gttgaaataa
    34561 aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc cttctgtcca
    34621 accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg ggaattgata
    34681 ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc
    34741 attattccat tctttttcca ttaattcttt ataacattca tttatccatt tttctggatg
    34801 tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta gcataatttt
    34861 agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc aaaatcaatt
    34921 ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc aaccactcct
    34981 gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat aaaagtgttt
    35041 tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt tcgtttaata
    35101 gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct atagttttcc
    35161 ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc aaaccctgtc
    35221 tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg ccagccttca
    35281 aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt agaggcaagt
    35341 acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg tccataggtt
    35401 ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg aatccctagc
    35461 attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga aaaaccaaat
    35521 tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa atttggccct
    35581 aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag caaattggct
    35641 tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc cgtccaatac
    35701 tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa tggagataac
    35761 gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat attcaggata
    35821 cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt gactaacccc
    35881 ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga ccaaaagctt
    35941 gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc atcttccgca
    36001 aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat ctggctaatg
    36061 ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc aaaaggatta
    36121 taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag ataacaattt
    36181 tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc aaatttttgg
    36241 ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca ttcatattca
    36301 aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa ttttttccat
    36361 aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac catacgctgg
    36421 tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc agcttttatt
    36481 cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg ccccgctccc
    36541 cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc taccccttga
    36601 tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg ttgatttatc
    36661 tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg atgtttcatt
    36721 gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat cgaagtaagc
    36781 cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt tctctatggt
    36841 atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt gcatagtgct
    36901 tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat cgtacagagg
    36961 aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca ctggtatcaa
    37021 tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt tcgattttct
    37081 gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt tttgtgtaac
    37141 gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa caagaattct
    37201 cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca tcaaattctg
    37261 cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta tctgcatgct
    37321 gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa tacagtaatg
    37381 gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc catgctacta
    37441 aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt aaccctagtt
    37501 gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt aagaataaaa
    37561 aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat aattgttctt
    37621 gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc tgccataaaa
    37681 taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt acgtctgtat
    37741 attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat tccgcatcta
    37801 tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt tcattcatgg
    37861 atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac gaactagatg
    37921 gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc cttaaattta
    37981 gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa aattagcata
    38041 attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt gataattaag
    38101 ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata ttgattccaa
    38161 cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat cggtacagcg
    38221 attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc catcagtgct
    38281 gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa
    38341 gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat gtaaaataaa
    38401 ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt gaacaacagt
    38461 ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga actggctgta
    38521 gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa gggataatta
    38581 tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt gaaattgatt
    38641 ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata taaatatggc
    38701 atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct gctttgtatc
    38761 ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata atataacaga
    38821 tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca tttcaaaact
    38881 tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat tggaagtccc
    38941 aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta aaactgtggc
    39001 cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc gaaatttcga
    39061 tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa gaccagctaa
    39121 aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca ttgatattcg
    39181 tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat tcaaaaatcc
    39241 attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag ccaccgttga
    39301 tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta actgtgctga
    39361 attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg atcctgccat
    39421 gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga tagctagata
    39481 attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa aagcaaacga
    39541 gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc
    39601 ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct ttattgtctt
    39661 gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa tcaccagtta
    39721 attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat
    39781 cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca tctgaataag
    39841 tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt tcatagtatt
    39901 tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag gccaatgagt
    39961 tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct gtcagcataa
    40021 atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca taaaaacttg
    40081 atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc agtggagcca
    40141 tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca taccagccgc
    40201 cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat
    40261 gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc gcttctttag
    40321 tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg gctagcatgt
    40381 tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta ccagtttttt
    40441 cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct tcaaaatcta
    40501 cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta aaatcactag
    40561 cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat ttttgatcat
    40621 aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa aaagcatctt
    40681 gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata tccggcatat
    40741 cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc gaagctacat
    40801 tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt gcagcatttt
    40861 caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg gcttttttta
    40921 tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat gcacttaagc
    40981 tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt ttcatgtcta
    41041 tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata ttaacggaat
    41101 tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct tttcattttt
    41161 gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga aaggaaagtg
    41221 gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact aacctattcc
    41281 atagataccc atcttcattt atctgctgct ccctattttc gactactaat aaatgaaagc
    41341 gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc ttccctttta
    41401 ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac acaaactttt
    41461 atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg acaatctttt
    41521 tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca gttcaaaatt
    41581 gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa ttcttttttc
    41641 atactatgcc aacagatgag cattcatttg ctttccttat tagttactta ttgtctgcaa
    41701 aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt attgacaata
    41761 ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga tgaattagtt
    41821 gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct tgaacaatta
    41881 gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa agtgaatgga
    41941 aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg tctcgtgcta
    42001 gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc gatccatcat
    42061 ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta tctatcctta
    42121 aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt tctgtctatt
    42181 caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata acatagtacg
    42241 atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt gtagctggcg
    42301 gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc attatctatg
    42361 aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta ggaggtgtga
    42421 tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca aaaaacaaca
    42481 tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag cgattaacag
    42541 ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg atcatctctt
    42601 ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa aacggctttg
    42661 tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac
    42721 aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag aatttcaagc
    42781 atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc ccttatgatc
    42841 cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc gttcatttag
    42901 aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca gatggctccg
    42961 tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat gaagtggtga
    43021 aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac cagttacaaa
    43081 ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt gtagtgactg
    43141 gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat atgctggcag
    43201 atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa
    43261 tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca
    43321 taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc tatccagaag
    43381 ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt ttaggtggac
    43441 aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta gcaatcaccg
    43501 gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca ccgtattcta
    43561 ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg acgaatatcg
    43621 tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg ttctttttag
    43681 atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa aatattccaa
    43741 tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt tacattactt
    43801 tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg ggtatcaaag
    43861 cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct aggagttatt
    43921 tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa
    43981 tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta
    44041 agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat
    44101 gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat
    44161 attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat
    44221 attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa tcgactggct
    44281 tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg taatcatatt
    44341 tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt gtgtattaaa
    44401 taatttaatt tttttgttcc tatttattta a
//
LOCUS       DACCCM010000014        41266 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCCM010000014 DACCCM010000000
VERSION     DACCCM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.014"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            214..498
                     /locus_tag="IVK78_000800"
     CDS             214..498
                     /locus_tag="IVK78_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP6374291.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IVK78_000801"
     CDS             complement(779..1198)
                     /locus_tag="IVK78_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP6374292.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IVK78_000802"
     CDS             complement(1200..1784)
                     /locus_tag="IVK78_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP6374293.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IVK78_000803"
     CDS             complement(1831..3306)
                     /locus_tag="IVK78_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP6374294.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IVK78_000804"
     CDS             3487..3798
                     /locus_tag="IVK78_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374295.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IVK78_000805"
     CDS             complement(3913..6249)
                     /locus_tag="IVK78_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374296.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IVK78_000806"
     CDS             6391..7059
                     /locus_tag="IVK78_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6374297.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IVK78_000807"
     CDS             7062..8105
                     /locus_tag="IVK78_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6374298.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IVK78_000808"
     CDS             complement(8342..9223)
                     /locus_tag="IVK78_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6374299.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IVK78_000809"
     CDS             9382..10110
                     /locus_tag="IVK78_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP6374300.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IVK78_000810"
     CDS             10266..10583
                     /locus_tag="IVK78_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6374301.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IVK78_000811"
     CDS             10576..11184
                     /locus_tag="IVK78_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP6374302.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IVK78_000812"
     CDS             11200..12030
                     /locus_tag="IVK78_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP6374303.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="IVK78_000813"
     CDS             12168..12656
                     /locus_tag="IVK78_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP6374304.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="IVK78_000814"
     CDS             12707..13579
                     /locus_tag="IVK78_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP6374305.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="IVK78_000815"
     CDS             13755..14117
                     /locus_tag="IVK78_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6374306.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="IVK78_000816"
     CDS             14126..14878
                     /locus_tag="IVK78_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374307.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="IVK78_000817"
     CDS             14892..16694
                     /locus_tag="IVK78_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374308.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="IVK78_000818"
     CDS             16971..18011
                     /locus_tag="IVK78_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6374309.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="IVK78_000819"
     CDS             complement(18063..20207)
                     /locus_tag="IVK78_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6374310.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="IVK78_000820"
     CDS             complement(20564..21937)
                     /locus_tag="IVK78_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6374311.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IVK78_000821"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IVK78_000821"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP6374312.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IVK78_000822"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IVK78_000822"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP6374313.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="IVK78_000823"
     CDS             complement(23385..24398)
                     /locus_tag="IVK78_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6374314.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="IVK78_000824"
     CDS             complement(24479..25078)
                     /locus_tag="IVK78_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6374315.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="IVK78_000825"
     CDS             25264..25965
                     /locus_tag="IVK78_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374316.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="IVK78_000826"
     CDS             25975..28686
                     /locus_tag="IVK78_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6374317.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="IVK78_000827"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="IVK78_000827"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP6374318.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="IVK78_000828"
     CDS             complement(30825..32006)
                     /locus_tag="IVK78_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6374319.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="IVK78_000829"
     CDS             32258..33781
                     /locus_tag="IVK78_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6374320.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="IVK78_000830"
     CDS             complement(33850..34578)
                     /locus_tag="IVK78_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6374321.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="IVK78_000831"
     CDS             complement(34591..35871)
                     /locus_tag="IVK78_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6374322.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="IVK78_000832"
     CDS             complement(36012..36614)
                     /locus_tag="IVK78_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP6374323.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="IVK78_000833"
     CDS             36859..37065
                     /locus_tag="IVK78_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374324.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="IVK78_000834"
     CDS             37377..38096
                     /locus_tag="IVK78_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6374325.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="IVK78_000835"
     CDS             complement(38177..38860)
                     /locus_tag="IVK78_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP6374326.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="IVK78_000836"
     CDS             39078..41264
                     /locus_tag="IVK78_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6374327.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13384 a         7322 c         8275 g        12285 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcatg
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagttg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctggtttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggaa cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatatc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgata
//
LOCUS       DACCCM010000015        41151 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCCM010000015 DACCCM010000000
VERSION     DACCCM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.015"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            237..479
                     /locus_tag="IVK78_000837"
     CDS             237..479
                     /locus_tag="IVK78_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP6374328.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            476..946
                     /locus_tag="IVK78_000838"
     CDS             476..946
                     /locus_tag="IVK78_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6374329.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1074..2288
                     /locus_tag="IVK78_000839"
     CDS             1074..2288
                     /locus_tag="IVK78_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6374330.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2998..3186)
                     /locus_tag="IVK78_000840"
     CDS             complement(2998..3186)
                     /locus_tag="IVK78_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374331.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3277..3486
                     /locus_tag="IVK78_000841"
     CDS             3277..3486
                     /locus_tag="IVK78_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP6374332.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3598..5211
                     /locus_tag="IVK78_000842"
     CDS             3598..5211
                     /locus_tag="IVK78_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP6374333.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5246..6457
                     /locus_tag="IVK78_000843"
                     /pseudo
     CDS             5246..6457
                     /locus_tag="IVK78_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6480..7406
                     /gene="glsA"
                     /locus_tag="IVK78_000844"
     CDS             6480..7406
                     /gene="glsA"
                     /locus_tag="IVK78_000844"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP6374334.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7614..9074
                     /locus_tag="IVK78_000845"
     CDS             7614..9074
                     /locus_tag="IVK78_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374335.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9295..11058
                     /locus_tag="IVK78_000846"
     CDS             9295..11058
                     /locus_tag="IVK78_000846"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP6374336.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11142..11600
                     /locus_tag="IVK78_000847"
     CDS             11142..11600
                     /locus_tag="IVK78_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP6374337.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11709..12392
                     /locus_tag="IVK78_000848"
     CDS             11709..12392
                     /locus_tag="IVK78_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6374338.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12539..13153
                     /locus_tag="IVK78_000849"
     CDS             12539..13153
                     /locus_tag="IVK78_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP6374339.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13169..13375
                     /locus_tag="IVK78_000850"
     CDS             13169..13375
                     /locus_tag="IVK78_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374340.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13916..15046
                     /locus_tag="IVK78_000851"
     CDS             13916..15046
                     /locus_tag="IVK78_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6374341.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15138..15893
                     /locus_tag="IVK78_000852"
     CDS             15138..15893
                     /locus_tag="IVK78_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6374342.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15951..17990
                     /locus_tag="IVK78_000853"
     CDS             15951..17990
                     /locus_tag="IVK78_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP6374343.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18099..19250)
                     /locus_tag="IVK78_000854"
     CDS             complement(18099..19250)
                     /locus_tag="IVK78_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6374344.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19404..19934)
                     /locus_tag="IVK78_000855"
     CDS             complement(19404..19934)
                     /locus_tag="IVK78_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374345.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20033..20383)
                     /locus_tag="IVK78_000856"
     CDS             complement(20033..20383)
                     /locus_tag="IVK78_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP6374346.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20540..22069)
                     /gene="ahpF"
                     /locus_tag="IVK78_000857"
     CDS             complement(20540..22069)
                     /gene="ahpF"
                     /locus_tag="IVK78_000857"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP6374347.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22079..22642)
                     /gene="ahpC"
                     /locus_tag="IVK78_000858"
     CDS             complement(22079..22642)
                     /gene="ahpC"
                     /locus_tag="IVK78_000858"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP6374348.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22786..23448)
                     /locus_tag="IVK78_000859"
     CDS             complement(22786..23448)
                     /locus_tag="IVK78_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6374349.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23632..25278
                     /locus_tag="IVK78_000860"
     CDS             23632..25278
                     /locus_tag="IVK78_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6374350.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25269..26351
                     /gene="uxuA"
                     /locus_tag="IVK78_000861"
     CDS             25269..26351
                     /gene="uxuA"
                     /locus_tag="IVK78_000861"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6374351.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26459..28012)
                     /locus_tag="IVK78_000862"
                     /pseudo
     CDS             complement(26459..28012)
                     /locus_tag="IVK78_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            28160..29301
                     /locus_tag="IVK78_000863"
                     /pseudo
     CDS             28160..29301
                     /locus_tag="IVK78_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29427..30506)
                     /locus_tag="IVK78_000864"
     CDS             complement(29427..30506)
                     /locus_tag="IVK78_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6374352.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30609..31049)
                     /locus_tag="IVK78_000865"
     CDS             complement(30609..31049)
                     /locus_tag="IVK78_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6374353.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31106..31516)
                     /locus_tag="IVK78_000866"
     CDS             complement(31106..31516)
                     /locus_tag="IVK78_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6374354.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(31696..32130)
                     /locus_tag="IVK78_000867"
     CDS             complement(31696..32130)
                     /locus_tag="IVK78_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6374355.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32230..33078)
                     /locus_tag="IVK78_000868"
     CDS             complement(32230..33078)
                     /locus_tag="IVK78_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6374356.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33467..35233
                     /locus_tag="IVK78_000869"
     CDS             33467..35233
                     /locus_tag="IVK78_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP6374357.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35335..36594
                     /locus_tag="IVK78_000870"
     CDS             35335..36594
                     /locus_tag="IVK78_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6374358.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36741..38042
                     /locus_tag="IVK78_000871"
     CDS             36741..38042
                     /locus_tag="IVK78_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6374359.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38045..38896
                     /locus_tag="IVK78_000872"
     CDS             38045..38896
                     /locus_tag="IVK78_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6374360.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38909..39724
                     /locus_tag="IVK78_000873"
     CDS             38909..39724
                     /locus_tag="IVK78_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP6374361.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39875..41041
                     /locus_tag="IVK78_000874"
     CDS             39875..41041
                     /locus_tag="IVK78_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6374362.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT        13970 a         7121 c         7991 g        12069 t
ORIGIN      
        1 aataagaaat tgattgtcgt tttcttttgc agaaatttaa gtgcaggcaa ttatggtagt
       61 tgtcgtaatt gctgtcagct cagtgtttat cactgatggt gtgaaagatt tacggttgga
      121 gatgttcaat aagattattg gtgcttaatt atttttattg tagtgctgat atgagaatgt
      181 tataattggg gtataaaaca tacaaataaa tttgctgaaa aatgaggtat caaaatatgg
      241 aaaatgaatt actggaaaac ttaggaagac ttcacacaac tgaacttggc gttattagaa
      301 ttaaaaagaa cttatctttg aatgtagaaa atgtaattga atggtgcaaa gaaaaaatta
      361 gcctttctaa tgcggaaatc acaagaaaag gaaaaaactg gtatataacg attgataatt
      421 gtattataac ggtaaatgcg tacagttata ccgttatcac tgctcacaag gtaaaatgat
      481 aaatttttct ataaaggaat taaacgcatt tgcagttatt gggcaggaag tagaactgac
      541 taattatcaa aaaaagaata ttcagattag tacacagttt tggagaaagt ttaacagtag
      601 tttgaaaaaa gcgtatcttt cacaatcagg aaattgggta aaatatgctt ttatggaaag
      661 aagaaatgga aaactttatt atttctgttc tattccccaa agaaccatta ccccagaggg
      721 ctttctgtat aaagaaatac cgtcttataa atatttggtt gtggagcata ttggtgctat
      781 ggataaaata tatgaaactt atggcaagat ttatgaagaa attattccta gtacatcgta
      841 tattcctata aaagatatta tcctacactt tgaaaaatac gattatcgtt ttcattggaa
      901 tagtgataat tcagttattg aaatttggat acctattcaa gattagaagc aattttatat
      961 ccatacacac aaagcagtta gtcttaggat tgactgcttt ttctatacag atttttaatg
     1021 acaggcaatt atcaattatg gtagttgcct tttttgattg gaggaattga agaatgaatg
     1081 ataaaaactt tattgaagaa ctaagacaaa agcgtgagga atacggagta acacaaacaa
     1141 ggcttgctgt tgcctgtggt atcagccgtg aatattacaa ccgcattgaa aaaggaaaac
     1201 agccattgaa tgacgaatta agagaaatca tagaaaaaca gattgagcgt ttcaatccgc
     1261 aagaaccact attcttattg attgattact ttcgtgtccg ctttcctact acggacgcat
     1321 tggcgattat ccgtgatgta ttacaactga aatctaatta catgctttat gaagattatg
     1381 gaaaatacgg atatgaaagc aaatatgtac ttggcgacat caatatcatg tgttccatgc
     1441 aggagcattt aggtgtttta ttggaactga aaggcaaagg ctgtcggcaa atggaatgtt
     1501 atctgctggc acaggaacgc tcatggtatg acttcatgct ggattgtatg acggctggtg
     1561 gtgtgatgaa acggcttgat ttggctatca atgataaagc aggaatattg gatattccaa
     1621 agttaaagga aaaatacaag gctggcgaat gtatgtccta cttccgtatg cagaaagatt
     1681 acagcggtac agaaaaatgc ggtagcgatt taccaaagaa tacaggagaa accttatatc
     1741 tcggttcaac aagtagcgaa ttatatatgt gtgcttatca gaaaaattat gagcagtatg
     1801 tcaagaatgg cacagaaatt gaagatacgg agattaagaa ccgttttgaa atacgcatga
     1861 agaacgaacg agcctattat gcggttgtag atttactgac ctatcgggac gctgaacgca
     1921 ccgctttttc tatcatcaat cattatgtcc gctttgttga cagagaggac gacaagccaa
     1981 aaagtcaatg gataacaaat gatgattggg catggtttgt aggggaaaac agagagccga
     2041 tacgcttaac cacaaagcca gagccttaca ctttacaaaa agcgttgcat tggctacaaa
     2101 gacaagtcgc accaaccata aaaatggtac aggcattaga cagagaaaat cgtacaacga
     2161 tactgaaaga tatgattgag caggcagaac taaaagacaa gcataaacat ttattacaat
     2221 tagagaaatc aaccatagaa gaacgtatag ataccgctgt tccacaagag aatgacggta
     2281 ttttttaatg taattgaaaa atttcagatt tagtcagcaa agagctgttt tcattcccta
     2341 tgtgaagtga aagaagaaaa ataaattttt tattaaatct gcaacaaaat ggctacttgc
     2401 gtacagatat ggtgcgagaa aaggaaatct aaactttttt tgaaaatagg tcattaaatc
     2461 ggggcttgct gtccctatat gaagtgaaag aagaaaaaag ttttgaaaaa atgacggaaa
     2521 ttcgtgatta gtggacagtc ttctgtgaaa agaggaaaaa catcatcatt ttgtattcaa
     2581 acaggcatta gcagttgagt gttgatgtga aaagatgaaa atactttgtt ctcaacttaa
     2641 caatttaagg tttccgttcc ctatatggtg caaagaaaat atttcattct attgttggca
     2701 gtaacggtat ggtttgggta gttagggtgt gaaaaggaaa atattctttt ttcatcatca
     2761 acttagcaaa atgacatttt ctatccctat atggtgcatg aaaagaagtt aaaatttttc
     2821 catttaaact tagcaaatgg acgtttgctg tacagatagt agtgaaaaag gagaaaatca
     2881 accgttgatt gccaaaatga accgttccat ttgtactaat aatggaaaaa gttttggatt
     2941 ttggttacgt ttctcctctt ttccaacgcg atatatagga aagacgattt ttttctttca
     3001 atcgttcttt gacaactgaa taaagctatt caatacgttt gacagacagc tagccgttga
     3061 aacaaatacg ccacgacctt tcccctgcaa gagcgagcga aaacctattt gtgttcttgt
     3121 aaaagatttg tcctctttta tcgccatgac ctgtactgtc agataatgat actcccgtac
     3181 agccacagct tgagcattaa gagcgtcgca cgcaatgggt acggctacat aagaactatg
     3241 cagaggtgga actcctgtgg gctatgacaa aagccgttga attgcttaga aagattttac
     3301 agaaaggggg tatgtgttat tgataatgct gtaaaaacaa ttcaaaagaa aaatggcaag
     3361 tgtggcattg gcaacagagg aaagacaaag attgtagtaa aagagcattt ttccgaaaag
     3421 ggcaaaacaa tggaagaact tctaactgat gtcatgctgg aaaaagcaaa gcagacaatc
     3481 gcataaaatc ggtgcagttg taagaaatag attgaatgac aaaaagtacc cgtgttatac
     3541 tttacttgca agcaggtatt gtaaacacgg gttagttata aaggaggata actgacaatg
     3601 aaacaacaga tttacaatac tgcactttat cttaggttaa gccgagatga tgaattacag
     3661 ggcgaaagtt ccagcattac cacacagaga agtatgttgc gtttatatgc aaaagaacat
     3721 catttgaatg tcattgatga atatattgat gacggctggt cgggaacaaa ttttgacaga
     3781 ccgagttttc aaagaatgat tgaggatata gaggcaggaa aaatcaactg tgttgtaacg
     3841 aaagaccttt cccgtcttgg cagaaattat attatgacag gacaatatac agaattgtat
     3901 ttccctagtc ataatgtccg ctacatagcg attgatgacg gtgtagacag cgaaaaaggc
     3961 gaaagtgaga ttgcaccatt taagaacatc atcaatgaat gggtggcaag agatacaagc
     4021 cgtaaagtca aatcagcatt taagacaaag tttgcggagg gggctcatta cggtgcttat
     4081 gctccgttag gatataagaa acaccctgac atcaaaggaa aactgttggt tgatgatgaa
     4141 acaaaatgga ttattgaaaa aatcttttcc ctagcctatc aaggttacgg aagtgcaaaa
     4201 atcacaaagc agttaagagc agaaaaagtt ccgacagcgt catggctaaa ttttacaaga
     4261 tacggtactt ttgcacatat ctttgaggga aaacccgaaa gtaagcgtta tgagtggacg
     4321 attgctcatg tcaaggcgat attgaaaagt gaggtttata tcggaaacag tgttcacaat
     4381 atgcagtcta cggtatcgtt caagagcaaa aagaaagtgc gtaagcccga aagtgaatgg
     4441 tttcgagtag aaaacactca cgagccgatt attgacaagg aagtgttcta tcgtgtgcag
     4501 gagcagataa aatcaagacg cagacagaca aaggaaaagg caacgccgat ttttgcaggg
     4561 cttgtcaagt gtgcggattg tggttggtct atgagatttg gaacgaataa gacaaataaa
     4621 acgccttaca gttattatgc ttgcagttac tacgggcagt ttggcaaagg taattgttct
     4681 atgcactaca ttcgttatga tgtgctgtat caagccgtat tggaacgctt gcagtattgg
     4741 gctaaggcag tacagcagga cgaagaaaag gtattgaaca agatacagaa agctggcaat
     4801 gcagagagaa tacgggaaaa gaagaaaaag gcaagtacat tgaagaaagc cgagaacaga
     4861 caaaatgaga ttgaccgttt atttgcgaaa atgtatgaag atagagcctg tgagaagata
     4921 acagagcgaa attttgtgat gttgtccagc aagtaccaaa aagaacagat agaactggaa
     4981 cagcagataa caagcctaag agaagaacta agtaaaatgg aacaggatat gataggtgct
     5041 gaaaagtgga ttgagttaat caaggaatat tccgtaccaa aggaactgac agcaccatta
     5101 ttaaatgcca tgatagaaaa aatcctcata catgaagcaa caacgaatga ggataacgaa
     5161 agaatacagg aaattgagat atattaccga tttattggaa gagttgagta atcaacaaga
     5221 gtaatatttt taactaaggg aaaccggcgg tgtctatact tgggtcagtc gcacattggg
     5281 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     5341 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     5401 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     5461 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     5521 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     5581 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     5641 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     5701 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     5761 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     5821 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     5881 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     5941 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     6001 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     6061 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     6121 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     6181 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     6241 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     6301 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     6361 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     6421 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     6481 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     6541 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     6601 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     6661 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     6721 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     6781 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     6841 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     6901 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     6961 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     7021 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     7081 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     7141 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     7201 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     7261 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     7321 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     7381 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     7441 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     7501 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     7561 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     7621 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     7681 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     7741 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     7801 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     7861 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     7921 cgagtacaga actggctgat cttgaattta tttctttgag taaagtatat acaatcaaaa
     7981 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     8041 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     8101 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     8161 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgagaaatag
     8221 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     8281 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     8341 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     8401 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     8461 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     8521 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     8581 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     8641 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     8701 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     8761 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     8821 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     8881 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     8941 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     9001 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     9061 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     9121 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     9181 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     9241 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     9301 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     9361 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     9421 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     9481 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     9541 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     9601 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     9661 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     9721 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     9781 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     9841 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     9901 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     9961 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
    10021 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
    10081 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
    10141 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
    10201 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
    10261 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
    10321 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
    10381 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
    10441 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
    10501 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
    10561 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
    10621 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
    10681 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
    10741 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
    10801 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
    10861 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
    10921 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
    10981 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
    11041 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
    11101 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
    11161 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
    11221 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
    11281 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
    11341 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
    11401 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
    11461 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
    11521 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    11581 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    11641 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    11701 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    11761 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    11821 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    11881 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    11941 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    12001 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    12061 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    12121 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    12181 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    12241 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    12301 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    12361 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    12421 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    12481 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    12541 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    12601 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    12661 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    12721 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    12781 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    12841 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    12901 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    12961 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    13021 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    13081 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    13141 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    13201 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    13261 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    13321 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    13381 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    13441 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    13501 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    13561 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    13621 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    13681 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    13741 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    13801 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    13861 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggattttggg
    13921 tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg
    13981 tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt
    14041 tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc
    14101 taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg
    14161 gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt
    14221 ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga
    14281 cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt
    14341 tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt
    14401 catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc
    14461 tgcattgctg attggagcag caggcggggt attatattta ggaaataaag gttggcttcc
    14521 acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag
    14581 tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt
    14641 tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga
    14701 ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt
    14761 cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca
    14821 agctggattg tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt
    14881 gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg
    14941 aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa
    15001 gatacaggcc gaagagttgg cgctctatcg acctgaaaaa caataaagag aaactgagaa
    15061 actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt
    15121 agaaagagga attcgcaatg aaagtatctc aaaaaatcat attatctatt ttgttactgc
    15181 ttacattagt agttggctat ttttcgattg agtttatcca tggattttcg tctgcaaaac
    15241 agagttcaac tgtgaaaaag gtagagccga aaagtatccc taccacacta aatgtggctt
    15301 tgattggttc agatgctcgt tcgaaagacg aaaacggtcg ctcggattca cttctggttg
    15361 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
    15421 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
    15481 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
    15541 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
    15601 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg
    15661 atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga
    15721 ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt
    15781 caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc
    15841 cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac
    15901 aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa
    15961 gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag
    16021 cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag
    16081 ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag
    16141 ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct
    16201 tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc
    16261 taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca aagatgaata
    16321 cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc
    16381 aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag
    16441 taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat
    16501 tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag
    16561 gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta
    16621 tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca
    16681 ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat
    16741 attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag
    16801 aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg
    16861 agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg
    16921 cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca
    16981 aattgacaat cgatgcgaag gcacaaaaaa cagcttttga cagtctagga ggaaaagctg
    17041 gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa
    17101 gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag
    17161 aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt
    17221 ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt
    17281 tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatcttat caagcaacgc
    17341 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
    17401 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
    17461 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
    17521 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
    17581 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac
    17641 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
    17701 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta
    17761 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
    17821 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
    17881 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
    17941 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
    18001 tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt
    18061 tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata aacttttttt
    18121 gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa
    18181 ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa
    18241 gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa
    18301 ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc
    18361 aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca
    18421 ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact
    18481 tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag
    18541 ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg
    18601 ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat
    18661 ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa
    18721 gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg
    18781 actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact
    18841 acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc
    18901 ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa
    18961 acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc
    19021 aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc
    19081 gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa
    19141 acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag
    19201 ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt
    19261 cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
    19321 tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattgttggt ttttatacgg
    19381 tctattttaa taactaaaaa tgattaacgt atagtcattt ttcttattct gatgaaattg
    19441 ataatctcct ggatgcccat gaaaatcaaa ataaagccga atatttggaa tagaagcatc
    19501 aaacttttga atggattgaa caataagacg aatccgccaa tcaacaacag aatactacta
    19561 taaacactcc aacttttagc ttttgtattc atgctgaaga acaactggaa caagctgttg
    19621 atgataacag ctaatccaag aagaatcggc aggatagaaa cgattgcagg tgcaaaagca
    19681 tagacgatca gagctaagat caggaaaaaa agaccactga acagaccgat actccaagaa
    19741 cctgttttcc ttttaaaacg gtaagcctca aaaagattca acaacccaag caaaacaaga
    19801 taggcggtga tcagataacc gataaaatga aaaacagcag aagggtttag cacaatcgca
    19861 atcccagaaa taatataaac agcagctctt aataaggcgt atttttgaat attttgttta
    19921 atcgtctcaa acatataaga aaacctcctt tgacttattt taacgtgaac tagtcatttt
    19981 accaaattaa atgttacaaa atgagcgaag gagaagaata tctctggaaa aatcaacttc
    20041 tgaggatctt ttccattttt ttgccttttg ctagctcatc gatcatttta tctaaataac
    20101 gaatttcttt catcaagtta tcctcgatgt cttctactcg tacaccacaa acaacacctg
    20161 taatcaagga acgtgaagga ttggggacag gggcttccgc aaaaaattgt tcgaaagtca
    20221 cttgtttttg tagttgatat tctagttctg tttgggaata acccgtcagc caacaaatga
    20281 tttcatctac ttcctctttt atacgatctt ttctctccgc ttttgctaca tacataggat
    20341 aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc cataatcagt cacctttctt
    20401 tttttcaaat tatttactac ttatagtata taagaaaaag agaagaaaaa gcgattattc
    20461 aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat ccttttttcc gctctctgac
    20521 tttttacaat cttcatagct caagtgcgca tcagataatc gaatgctcct aaagctgcag
    20581 tagctcctga acccattgaa acaatgattt gtttgtatgg actatctgta caatcaccag
    20641 ctgcgaaaac gccggggata ttcgttgcgc catttttctc ggtcactatt tcatttcttg
    20701 gattcatttc gatcgttcca tctagccaat ccgtattagg gagtaagccg actagaatga
    20761 atactcctga aacagctagt gtatgcatct ccatcgtacg gcgatcgata taggagagag
    20821 attctacctg attcgtaccg ctgatttctt tcgtcgctgc atgtttgata acagtgacat
    20881 tcgcaagaga gactagttta tcctgtaaga tttgatctgc ttttaattct ggtaagaatt
    20941 ctagaacata tacatgtttt gctaacccag ccaaatcgat tgctgcttca acaccagaat
    21001 taccaccacc gataacagca atctcttttc ctttaaataa agggccgtca cagtgtgggc
    21061 aataagcaat tcctttattt ttaaactctt tttcgcctgg tacattgata gaacgccagt
    21121 gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga tataccgtta gctaagccaa
    21181 tattaataaa atgttgctct ttatttaagg aaacggcctg ttgattcatc ataatgtcga
    21241 tagggtaact acgcacatga ctttctactt gctgcatcaa ttttggacct tctgtgtaag
    21301 aggtaccaat catattttca atccctagtg tttcaacgac ttgtccgccg aaagaatcag
    21361 cgacaagtcc tgtacggatt cctttacgtg ctgcatagat agctgcacta ctagctgctg
    21421 gtccaccgcc aatcactaat acatcataag gttctttttc aaacaactct tcttcaatca
    21481 aagggccagt gattttttca acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt
    21541 ctccatttaa aaacacagtt ggcactgcca tgattttttt atcatcgatt tctttttgga
    21601 acatcccacc ttcaatcatc gtatgagtaa tatttggatt taatacagct aatatattca
    21661 atgcctgtac aacgtcgggg caattatgac aagtcaagct aacataactg tcaaaatgca
    21721 atgtttgtgt tatttttttg attttaagac tcaaagattc actgattttc ggtgcccgtc
    21781 cactaatttg taacatagcc agtaaaaaag aggaaaattc atgaccgagg gggataccag
    21841 caaaaacgat tccactttct ttgccaactg gattgataga aaaactaggt gtgcgcatcg
    21901 aatctctttt ttcccaagag attttaggag acatttctgc tatttccttc gtaaattcag
    21961 ctagtttttg actattttca ctattatcca aactaacaga aaaaacaata ggagattcaa
    22021 tcagtgctaa atattgttcg agattttgtt tagtttcttt gtctaacata tccaccacct
    22081 aaatctttcc aacaagatca aaacttggtt tcaatgtttc gccactttct ttccattttg
    22141 ccggacaaac ttcacctggg tgggtacgta catattgagc agcgcgaatc ttatcaatca
    22201 gtgcactagc gtcacgaccg attccatctg cattgatttc catagcttga acgattccgt
    22261 ctggatcaat aataaatgtt cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa
    22321 aaatacgaga aatctgatga gacggatcac cgatcatcac gtattcgatt ttaccgatag
    22381 catctgatgt atcatgccat gctttgtgag taaagtgggt atcggtagag caagaaaaga
    22441 cttcaacacc taaagatttc aatgtactgt attgttcttg taagtcttct aattcagtag
    22501 gacaaacaaa agtaaaatca gctggataga agcaaagaat actccagttc cccataagat
    22561 ctttttcact gacttctaaa aattctcctt gatgataagc atgtgcatta aattcttcga
    22621 tttttttccc aatcaatgac atagtaaaac ctccaataat aattatttat ttataattat
    22681 tgtaatttag ttggagttgc aaagtcaatg gatattttgt tatttaataa agattactca
    22741 aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga aaatgctact gctggaaata
    22801 ctcgggataa cgttgagtaa cagtttcctg ttctttcagc atcatgtgta agtgcaatcc
    22861 cgtttgaaat cgagcttctt ctggtttttt atcaactaaa gccttgagta aatcataatg
    22921 ttgttccatg atcgtttccc atggaagccc ctgaatggtc actcgtaacc agcggaaacg
    22981 ttctaaatgg gtattatggt catctaacca gttccaaact tcttttcttc ctgcaatttc
    23041 gaaacaagct tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt catttatttt
    23101 gactgctttt tcttgttctt ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa
    23161 tttggaacaa cattcaatca tgatttgctc ttccaactgt tctcggacaa agcgagcatt
    23221 ttctgcagaa acaagattaa ttttggaaac atacgtacca ctttgaggta tcgtatacac
    23281 gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag
    23341 atgttcctcc agtgcctttt cagatatttt ccttccaggt tctaagtcag catagataat
    23401 ttgtttacga atcgcttgat aagcttgtga ttgcaagctg cttttcatct ttattcctcc
    23461 aaaatcattt ctatgaacgt agtataacag aagaggtctg aaaaacaatc tggtatgtca
    23521 cattaactag taaatttgta taaatagtaa aattttggtt gacatagcga tttttattgc
    23581 ataaaattca cttgaataca aaaactaata tgttagttta ggaggggaaa aatgattgat
    23641 ttaaacaact ggaggaactt tatccctgaa ttatctcaaa aagaagtaga aatacctcat
    23701 tttgaccgtg catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt
    23761 ggcggaaatc tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc
    23821 gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct
    23881 taccaagatt ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc
    23941 aaacgtatat tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac
    24001 tttgataaga tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt
    24061 actgaaaaag gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc
    24121 gatataaaaa atggtccaaa gaagccatca catacaatga gtattcttgc tgtgctactt
    24181 tataaacgtt ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca
    24241 caaaacggta gtcaattacg tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa
    24301 ggttatgtac aaaatacatt tatccaatac ttatcgaatg aagaaattgt tgcgtttcca
    24361 tggacgatga ttgatcgtat caccccgaat ccttccttgt cagttagcaa aatgttggca
    24421 aaagagggct tttcaaatat gtcgattttc caaaccgcaa aaggaaataa tattgctcca
    24481 tttgtcaata cagaggacat ccattattta atcatggaag atcattttcc caatggacgt
    24541 ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg caacggtaga taaagcagat
    24601 acgatgaagg tgacgacttg ccttaatcct ttgcatacgt ctttagctat tatgggttgt
    24661 ttactggggt ataggagcat agctgaggag atgaaagatc cagatttatc gctgctagtt
    24721 tctgaaatcg ggtacaatga aggcctccca gttgtggagt ctccaggtat cattgatcca
    24781 aagatctttc tggaagaagt aattactgtg cgcttttcta atccaaatat tcctaatatg
    24841 cctcagcgaa tcgccacgga tacctctcaa aaggtggcga ttcgttatgg tgagacgatt
    24901 aaaaaatata tggaaataga agttaaagac ccgcagacac ttgtattcat accattgact
    24961 ctcgctgctt ggcttcgata ccttattggc attgatgatc aaggaaaacc attcatcccg
    25021 agtccagacc cactattgac agaccttcag cataagcttt ccgatattca actgggtacg
    25081 caagatacat cacatattca tgagaaaatg gctcctattt tgtcaaataa agaaattttt
    25141 ggtgtagatc tttaccaagc agagcttggc agaaaaatag aagaaatgtt catcaagatg
    25201 gtagcaggac caaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga
    25261 aatttatcat ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag
    25321 atgaaatcag acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag
    25381 taggagaggt atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg
    25441 ggttaacact aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac
    25501 cttcccgtga gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg
    25561 gaatagaagt catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg
    25621 actaccgctt agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg
    25681 atccaagaga aatcgtcgcg cgcatcgaac attccagcaa tgaatttgga ttgccgggat
    25741 gggaacctga gcgcttagct cctatcaaat ccttgcttga agcctatgca tcagtagacg
    25801 aagaaaaact tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag
    25861 aggtaggggt caaaatggct gtacatccag atgatccgcc atactcgatt tttggattag
    25921 ctcgaatcgt aaagaatcga gaagatttag attggttgtg tcgcatagta gacagccctt
    25981 ataacggaat cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg
    26041 ggattttagc agaattttgc caaagagatc gaatcccatt tgcacatgtt cgaaatatta
    26101 aatttttagc taatcgagat ttctatgagg caccacataa atccgaatac ggttcattag
    26161 acatgtaccg catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg
    26221 atcatggaag aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag
    26281 cattaggtgc tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta
    26341 aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc
    26401 ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct
    26461 aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa
    26521 agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt
    26581 ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa
    26641 cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt
    26701 gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa
    26761 tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc
    26821 attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat
    26881 ccatataaaa ttgttcaatg gcggtttgga cttctttcaa tacgctatgt tcttgataaa
    26941 gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt
    27001 tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa
    27061 atacagtaca aagggactcc accatttctc ttgaataaag aacatgagga acgtttgctt
    27121 tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa
    27181 gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca
    27241 aaatatctac agagagatca ataaaaaagt caatatattc atactctgtg aattcttttt
    27301 gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata
    27361 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
    27421 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
    27481 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
    27541 ttctgccatt attagaaaag gagatagaca aaccgtacta tgctaaaaat gcattaatct
    27601 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactg aaactgataa
    27661 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
    27721 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
    27781 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
    27841 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
    27901 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
    27961 tattgctttt tttcttcaag ataaacgttt taatcgcttc ttgagtttgc atgaaagagg
    28021 ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga
    28081 caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa
    28141 atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt
    28201 aagcttatcg cttactataa atcagacagt ccacacagag gagacagaaa cagccaaatg
    28261 gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt
    28321 caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga
    28381 ctattcggga gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc
    28441 cactttgtcg attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt
    28501 taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt
    28561 atcagatgga ctaccgaacg atcatcattt aacgccgtat agtcagcggc agacaattga
    28621 gcaagattca gccatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga
    28681 aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga
    28741 aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca ataaatcaaa
    28801 acctgaaata acccctggag aaaaaccaga tgtaactcca gaaccagata cggattctgg
    28861 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
    28921 accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
    28981 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
    29041 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
    29101 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
    29161 tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt
    29221 atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt
    29281 aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa
    29341 agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc
    29401 atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc
    29461 gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc
    29521 aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc
    29581 ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg
    29641 acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct
    29701 atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg
    29761 aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta
    29821 gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta
    29881 ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt
    29941 aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag
    30001 atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca
    30061 atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata
    30121 tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg
    30181 atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata
    30241 aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat
    30301 cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg
    30361 gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga
    30421 cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa
    30481 taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa
    30541 aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc
    30601 tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga
    30661 tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga
    30721 ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt
    30781 ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt
    30841 aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc
    30901 tttttctttt cccataaaac taacatcaca agatactggg tataggtaag attcagcggc
    30961 ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat
    31021 acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta
    31081 tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat
    31141 ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg
    31201 tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg
    31261 tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt
    31321 tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca
    31381 attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga
    31441 atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag
    31501 aatacatttt tttcataaaa ttaccacctt taattatatt ttatacaatt gaattttaca
    31561 caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat
    31621 gaaacagacg cgctttctcg aatagcaaac atatacaaaa aatatcagag acaaaaaata
    31681 atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt caaaaattac
    31741 ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt
    31801 agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa
    31861 tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg
    31921 atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct
    31981 cgtaccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat
    32041 cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa
    32101 tacaaaatcg atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag
    32161 ttactagatt cttttattat accagctaaa gaaaacgcat tcaaaaaaat agcggattta
    32221 cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct
    32281 gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa
    32341 ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt
    32401 tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc
    32461 caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata
    32521 agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc
    32581 aaaaataaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt
    32641 attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga
    32701 gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga
    32761 caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaac
    32821 caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac
    32881 caacataacg atattcaaca ctaaaataac tatagctcgg tcaaagccta aagcagcttc
    32941 cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat
    33001 attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa
    33061 ttgtctcgtt tctttcattt attcacttcc ttaattcatt aataatactc ttatcttatt
    33121 ataacttact tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat
    33181 tattatgttt cattcttcta gaatatactt ctacgccaaa aactgttaaa aaacttttac
    33241 tttagaaaat tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga
    33301 agtagactaa aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa
    33361 attcggtatc aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgcttctct
    33421 atcacttata gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc
    33481 aatttatcat cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat
    33541 tcggcttcgt actaggaaaa atgatatcaa aaaagtatat gtgctatgtg gagatccata
    33601 taccataaca actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac
    33661 caatgttcat gattattggg aaattgaagt gacgagcgaa acaagtcgct tacagtatgc
    33721 gttccatgtt gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc
    33781 ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca
    33841 aatcgatcga ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc
    33901 cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag
    33961 taaagatcca gaccgagaag actttttcgg gggagatttg caaggtgtga tggatcattt
    34021 ggattacttg acggaccttg gcatcaatgg catctatttt tgtcccatat ttaaagccac
    34081 atccaatcat aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa
    34141 aaaactttta aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga
    34201 tgctgtattc aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg
    34261 agaaaaatcg atctataaag actggttcca catccgtttt ttcccagtag attcttatca
    34321 gatgactgac ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc
    34381 attcatgccg aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc
    34441 aacttattgg ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt
    34501 cgatcatcat ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta
    34561 tatccttgga gagatttggc attcctctca agcttggctg caaggggatg aattccatgc
    34621 agtgatgaat tatgcattta cagattcgat taaagattat tttgcaaaaa agaaaatcac
    34681 agcttcacaa atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa
    34741 cgaaggaaca ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca
    34801 aggtaacaaa gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc
    34861 ttgtatctat tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa
    34921 atgtatgata tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact
    34981 tgtttctcta aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat
    35041 agtaaatgat cgtgaagata agctctattt cacacggaaa ctagagggac agatcatcta
    35101 tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct
    35161 tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt
    35221 gatcatgtgt tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg
    35281 tgcccattat aataagggtg taacagcacg ttgaatacaa aggaggaaat gaagatgaaa
    35341 aaaagaagtg tgaaacagtt gggtttaggg ttaatctctt taggaatctt aggcggatta
    35401 gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta
    35461 acagtttccg ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa
    35521 aaagaaaacg atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca
    35581 ttagcgttag atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg
    35641 atcggtgcgt taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct
    35701 tacgatgaaa cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca
    35761 gtaatcgaga cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg
    35821 tttaaagatt tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga
    35881 aaaaatacag gatttttagc taaatggact gacttttatt attcctatgg tttaattgca
    35941 ggttacggtg gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat
    36001 aatgcaggtg ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca
    36061 aagggaatgc aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat
    36121 aaaactgcag caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc
    36181 aattatggtg tagccaaaat tcctactcta aataatggac agccttatca accgtttggc
    36241 ggaggaaaag gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg
    36301 cttgattatg taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc
    36361 cctgcaaacc aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct
    36421 gtgattgaac aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt
    36481 tggacaggtg cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa
    36541 tccgcagatg aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa
    36601 tgataaccgt ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc
    36661 gagatggaaa attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca
    36721 agtttagagg gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa
    36781 tcacgctttg ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag
    36841 gtatcgtttt tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc
    36901 atgcattggt aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta
    36961 ttttgattga aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt
    37021 tgaatctttt agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc
    37081 ggacgatgaa ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac
    37141 ttcccgctta tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca
    37201 ctgcatttac taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag
    37261 gggctaaaaa cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag
    37321 tcttttcgtg gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta
    37381 ttctcacagc ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca
    37441 ttttcttatt accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat
    37501 tcaacgatag cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt
    37561 ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta
    37621 tcttagtgca gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac
    37681 aatcgatttc agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc
    37741 gcttttcaaa catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac
    37801 agtatacggg gaatttcaat aatttctcca tgatctatct gtttaacaat ggcggtcccg
    37861 gaagcgtagg cggaggagct ggttctactg atattcttat ttcctggatc tataaactta
    37921 cgactggaac ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca
    37981 tcgttatctg tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt
    38041 aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac
    38101 ttactttttt cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag
    38161 tactgccttc aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac
    38221 tttaggaaac tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac
    38281 attgatcatt tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta
    38341 tacttatagt cgctatcgtt ttatcggccg caaatcaagt ttgatgttct ttttagtgat
    38401 tcagatggta ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga
    38461 agcgctcgat aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa
    38521 tacttggctg atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa
    38581 acttgatggg gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc
    38641 gatgctcgcc gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc
    38701 taagttcctg cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat
    38761 ttctgatgtg acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact
    38821 accgatttgt ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg
    38881 cggaacaaaa ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc
    38941 attacgtacg ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt
    39001 cttgatattt gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa
    39061 tcagatccaa gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca
    39121 aggaacccaa aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca
    39181 aacgatagct gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc
    39241 cgatcatgga acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa
    39301 atctggtata cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg
    39361 tacgcccgaa gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac
    39421 aatcatttta tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat
    39481 tttattcggg gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca
    39541 tacattcaaa gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc
    39601 agcagttgta gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat
    39661 aactgtcttg ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga
    39721 atgacaagtt taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt
    39781 ttttgaaaaa tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta
    39841 acgtaatagg gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca
    39901 attattttca cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa
    39961 acaatacgga gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc
    40021 aatgaaagag attacaagta acgcatcttt ggcactagga gaagcctata tggatcatcg
    40081 aatcgagatc gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag
    40141 ctttttacga ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca
    40201 aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg
    40261 gctagatcca actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga
    40321 acaagcacaa atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga
    40381 aacgttgctt gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta
    40441 taatgtcaag gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag
    40501 aataaaagaa gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact
    40561 aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga
    40621 aaatcttgaa ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt
    40681 gatccatgga atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat
    40741 tttccctggc ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga
    40801 tttgcaactg attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg
    40861 gacaaagaat tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta
    40921 tcgaatgtgg gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga
    40981 tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata
    41041 attgaggcaa aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc
    41101 agaagtgaac ttttttgtct aacttctagg attgtctcta aagcttagtt t
//
LOCUS       DACCCM010000016        39685 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCCM010000016 DACCCM010000000
VERSION     DACCCM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.016"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            36..2009
                     /locus_tag="IVK78_000875"
     CDS             36..2009
                     /locus_tag="IVK78_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP6374363.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            2015..2467
                     /gene="rplI"
                     /locus_tag="IVK78_000876"
     CDS             2015..2467
                     /gene="rplI"
                     /locus_tag="IVK78_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP6374364.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            2741..4108
                     /gene="dnaB"
                     /locus_tag="IVK78_000877"
     CDS             2741..4108
                     /gene="dnaB"
                     /locus_tag="IVK78_000877"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP6374365.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            4888..6180
                     /locus_tag="IVK78_000878"
     CDS             4888..6180
                     /locus_tag="IVK78_000878"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP6374366.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            complement(6459..7169)
                     /locus_tag="IVK78_000879"
     CDS             complement(6459..7169)
                     /locus_tag="IVK78_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP6374367.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(7166..8638)
                     /locus_tag="IVK78_000880"
     CDS             complement(7166..8638)
                     /locus_tag="IVK78_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6374368.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            8776..9249
                     /locus_tag="IVK78_000881"
     CDS             8776..9249
                     /locus_tag="IVK78_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6374369.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(9402..9953)
                     /locus_tag="IVK78_000882"
     CDS             complement(9402..9953)
                     /locus_tag="IVK78_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6374370.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            10058..10588
                     /locus_tag="IVK78_000883"
     CDS             10058..10588
                     /locus_tag="IVK78_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP6374371.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(11071..11514)
                     /locus_tag="IVK78_000884"
     CDS             complement(11071..11514)
                     /locus_tag="IVK78_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6374372.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(11530..12690)
                     /locus_tag="IVK78_000885"
     CDS             complement(11530..12690)
                     /locus_tag="IVK78_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6374373.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13197..14897
                     /locus_tag="IVK78_000886"
     CDS             13197..14897
                     /locus_tag="IVK78_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374374.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(14999..15832)
                     /locus_tag="IVK78_000887"
     CDS             complement(14999..15832)
                     /locus_tag="IVK78_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP6374375.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(16136..18301)
                     /locus_tag="IVK78_000888"
     CDS             complement(16136..18301)
                     /locus_tag="IVK78_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6374376.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            18561..20855
                     /locus_tag="IVK78_000889"
     CDS             18561..20855
                     /locus_tag="IVK78_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6374377.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            20848..21513
                     /gene="pgmB"
                     /locus_tag="IVK78_000890"
     CDS             20848..21513
                     /gene="pgmB"
                     /locus_tag="IVK78_000890"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6374378.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            21506..22513
                     /locus_tag="IVK78_000891"
     CDS             21506..22513
                     /locus_tag="IVK78_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6374379.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            22535..23554
                     /locus_tag="IVK78_000892"
     CDS             22535..23554
                     /locus_tag="IVK78_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6374380.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(23835..25203)
                     /gene="nhaC"
                     /locus_tag="IVK78_000893"
                     /pseudo
     CDS             complement(23835..25203)
                     /gene="nhaC"
                     /locus_tag="IVK78_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(25312..26733)
                     /locus_tag="IVK78_000894"
     CDS             complement(25312..26733)
                     /locus_tag="IVK78_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6374381.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(26976..28853)
                     /gene="tdc"
                     /locus_tag="IVK78_000895"
     CDS             complement(26976..28853)
                     /gene="tdc"
                     /locus_tag="IVK78_000895"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6374382.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(29144..30400)
                     /locus_tag="IVK78_000896"
     CDS             complement(29144..30400)
                     /locus_tag="IVK78_000896"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP6374383.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            30980..32281
                     /locus_tag="IVK78_000897"
     CDS             30980..32281
                     /locus_tag="IVK78_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6374384.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(32350..32949)
                     /locus_tag="IVK78_000898"
     CDS             complement(32350..32949)
                     /locus_tag="IVK78_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6374385.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            33203..34084
                     /locus_tag="IVK78_000899"
     CDS             33203..34084
                     /locus_tag="IVK78_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374386.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            34097..35707
                     /locus_tag="IVK78_000900"
     CDS             34097..35707
                     /locus_tag="IVK78_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP6374387.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(35784..36443)
                     /locus_tag="IVK78_000901"
     CDS             complement(35784..36443)
                     /locus_tag="IVK78_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6374388.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(36726..37391)
                     /locus_tag="IVK78_000902"
     CDS             complement(36726..37391)
                     /locus_tag="IVK78_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374389.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(37538..38143)
                     /gene="dhaL"
                     /locus_tag="IVK78_000903"
     CDS             complement(37538..38143)
                     /gene="dhaL"
                     /locus_tag="IVK78_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP6374390.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(38171..39154)
                     /gene="dhaK"
                     /locus_tag="IVK78_000904"
     CDS             complement(38171..39154)
                     /gene="dhaK"
                     /locus_tag="IVK78_000904"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP6374391.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(39156..39551)
                     /gene="dhaM"
                     /locus_tag="IVK78_000905"
     CDS             complement(39156..39551)
                     /gene="dhaM"
                     /locus_tag="IVK78_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP6374392.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT        11972 a         8033 c         7383 g        12297 t
ORIGIN      
        1 gtaaaatcaa attagtcata tatgggagga aaattatgga aaataaaaac aatcatttat
       61 ccccgaaacc tctcttcctt atcctgttta tattaatcga agtattggta gtcttaattt
      121 taccatttcg ttttgttagc gcttcggtca ttctattgat gaatttatgg ggtgttcgct
      181 attggttgaa aatgaaacgt tggcttattt taaataaacg tgaaaaaatc cagcaggctt
      241 cgactgtagc tgaaaccaat ctagcttatg tctcagaagt catgcctgta ggagtgattg
      301 cttatcagcc atcaagccaa aaaatcgaat ggctcaatcc attagcattg aaaatcaaag
      361 atcaaagtgg attaacagag gaagaattgc tagatacttt ttttactgca aaagaaaaaa
      421 agaaaaacca agtctatcta gcagataaaa catttcagtt tgatatggat acagtaaagg
      481 gtgttgttta ttttaatgat attacccaaa accagttgtt aaaactccat cagacgaaca
      541 gtcagccggt gattggtatt ttatctattg ataactatgc agatgccatt gataaattgg
      601 atgacaaaga gatttcttat ttgaactctt tcgtcactac actagtttct gactggatga
      661 atgaatacca tgtctttttc aaaagaatca acgcagaacg gtttttcttc gtagcacaga
      721 tgatcgatct gaaaaagatg atggataaaa aatttgattt gctggatcgc tttcgagcag
      781 aagctgaaaa acagagctcg cctatcaccc ttagtatggg gattgcttat ggctcaagaa
      841 ccctcgatga aatcggtgga gaagctcaga ataatttaga tgttgcttta gtccgtggag
      901 gcgaccaagt tgtcgtgaaa gaggtaaaag aaggagcaaa acctgtttat tatgggggga
      961 aatctgctaa gaatgtcaaa cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc
     1021 gtcggatttt tgatgaaaca ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg
     1081 atgctttagg ctctgctttt ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt
     1141 acatcgttat caacgagaac gagatcattc cagatgtaga acggtgtctt gaagaaatcc
     1201 ataagcatcc tgaattagaa atgcaactaa tctctccaga acgtgccata gagctgaaaa
     1261 aagaagagga cctcctcgtg atggtggatt accataaacc ttctctatcg atctctcagg
     1321 gcctatatga gcaatttgat aagattgtga tcattgatca ccatcgtcgt ggggaagagt
     1381 ttccaagtaa acctctgctg acttatatcg aatcttcagc ttcttcagct tcagagctag
     1441 tttctgagct gatccaatac caaagcagca aaacaaatcg cttagatcgt tttgaggcca
     1501 cattattgct tgcagggatg gttgtagata caaatagttt caatgtccgt acaactgcac
     1561 gaacttttga cgttgccagc tatttaaggt cttgtggagc agacgcatcc ttagtacaat
     1621 atttattgag ctcggattta gcgacatact tggagatcag caatttgatt gctggaagcg
     1681 actatgtgac ttctgatatt gttgtggcgg cagggacaga cgaaaaagag tataatagtg
     1741 ttatagcagc aaaaacagca gatacattgc tttcgatgat caatatcaat gcagctttcg
     1801 tcattacaaa gcggacagac ggcttgattg ggatcagtgc cagaagtacc ggcacgatca
     1861 acgtccaaat catcatggaa tccttaggtg gcggaggaca cttcacaaac gcagctactc
     1921 aactggaaaa tacgacagtt gaggaaacaa aacagaagct atttgctgca atcaaagaaa
     1981 atatgaatca aatatatgat aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt
     2041 aaaaggaaaa ggtaaaaaag gggacatcaa agaagttcca gcaggttatg cacaaaatta
     2101 tctaatcaaa aataacttgg caaaagaagc aacgaaaagc agtatcagtc agttagcagg
     2161 acaaaagaaa gcacaagaaa aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa
     2221 agaattcttg gaaaaagaag atactgtagt cgaattgaag gcgaaagcag gggaagacag
     2281 ccgtttgttt ggttcgatcc cttcaaaaca aattgctgaa gggttaaaca aacaatacaa
     2341 aatcaaatta gataaacgaa aaatcgaatt agaaaatccg atccgttcac taggctacac
     2401 aaacgtacct gttaagcttc atcatgaggt gacggctacg atcaaagttc atgtcgtaca
     2461 agaataacaa atcttgatag cgatgttcag ctggtaagta taaccagaag gaaaaaagac
     2521 aaaccgaaat ttatctgaga aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt
     2581 tcagattgat ttcggtttct tttttgggaa cagcaaaata gcataaagaa aacgagtata
     2641 ataaaagaga actatttttt tattacactt tcggtacgaa tgatgacttt tctctcattt
     2701 tcttgtaaaa tgaagcaatt gaagacaagg agcgatacgt atgaatgaag tgtggcagga
     2761 tcgaatccca ccgcaaaata ttgaagctga acaagcggtt ttaggctctg tttttcttga
     2821 cgcagaaacg atcattgatg ccatggagta catagagcca aaagactttt atcgtcgtgg
     2881 ccatcaaatt atttttgaga caatgatcga gttaaatgat cgtaatgaag cgattgacgt
     2941 ggttactgtc aaagaccggc tggaacaagc caatttgtta gaagatgccg gtggtttaag
     3001 ttatctctca gacttggcat tagcagttcc gacagcagca aatatcgtct actatgcgaa
     3061 aatcgttgaa gaaaaatcat tgctgcgtac attgatccag acagcaacag gaatcgttac
     3121 gaaaggattc gagcaaggag aagaagttca aacaatttta gatgatgccg aacgcagtat
     3181 cttggaggtt tcggaaaaaa gaaatcgaag cggcttttta tctattgctg aagtactgaa
     3241 ctccacgatt gaaaatatcg atcgactagc tcaaaacaat gaagaaatca caggtctacc
     3301 aactggctat caaggtctgg ataaaatgac agcaggtttc cagcccgaag aactaatcat
     3361 tcttgctgca cgtccagcgg taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt
     3421 aggaacaaaa acagatcgtt ctgttgcgat atttagtttg gaaatggggg cagaatctct
     3481 tgtaaatcgg atgctttgtg cagaaggttc gattgaggcc agccatttac gtacaggtca
     3541 gctttcggaa gaagaatggt cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa
     3601 tatttttatc gatgatacac cagggatcaa aatctctgaa atccgtgcga aatgtcggaa
     3661 actagcgcaa gaaaaaggca atctaggctt gatattgatc gattatttgc agttgattga
     3721 aggtaacggt aaagagaacc gtcaacaaga agtatcagat atctctcgcc aactgaaaaa
     3781 actggcgaaa gagctgaaag tacctgtcat tgctctttca cagttatctc gtggagtaga
     3841 acagcgacaa gacaaacgac cagtgctaag tgatattcgt gaatccggat caattgaaca
     3901 agatgcggac atcgtagcct tcttgtatcg agatgattat tatgaaagag aaggcggaga
     3961 tgacgatgat ggaaacgagc caaaaaatga caacgtcatc gaagtcatca tcgagaagaa
     4021 ccgaagcggt gcacgaggaa cagttgaact actgtttatc aaagaataca ataagttttc
     4081 gtcgctttcg ccaagagaag aattttaaag aagaggttgt gagccttgag caatcccgag
     4141 agctctcgta agaagatgca aggagtaacc gcaggaacga ctagagtact taatttaaac
     4201 agatctccaa agggattgtg acaaaagtct tgtcacagtc cctttttccg aataaacggt
     4261 gttcaatcag ctgacccttg gtattagctc agcaaacgtc aaaatatgag gaactatttt
     4321 gacgtttgtt tctctaatac tcaggtcaaa acgctgatct tacgacctct tttccttaaa
     4381 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tcatcaaaca tgagaagcag
     4441 ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctttctta
     4501 aacggtgttc aaaagcttgc atctgcggta atacgcccaa tcaaacgaaa aatatgagaa
     4561 gctatttttg tttggctgtt cttattactt gatgcaggac agctttttca caacctctga
     4621 ttcacggtgt tccaaaagta gcttcttcga aaaataagtc gaaatttcac aaaaatttga
     4681 gaagcaattt tcgtacattc cttcttattt cttgaagctg accacttctg ttacaacctc
     4741 ttttttaata accattctaa aaagataaag ttcgtttttt aagcgttttt ataaaataaa
     4801 aataaaaatc attcgatttt atttgaaata tgtatctatt attgatacaa tggttcagga
     4861 ttaaataata tagaacgagg tgttcacatg tcatctgtag tagtagtagg tacgcaatgg
     4921 ggcgatgaag ggaaaggaaa aatcacagac tttctcagtg aaaatgctga agtgattgca
     4981 cggtaccaag gtggcgacaa tgcagggcac acaattaagt ttaacggtgt gacttataaa
     5041 ctgcatttga ttccttctgg gattttttat aaagacaaga tcagtgtgat cggaaacggc
     5101 gttgtagtca atcctaaatc attggttaaa gaattagctt atttacatga aaaaggtgta
     5161 gaaacaagta atttgcgtat ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa
     5221 ttggaccaat tacaagaaga tgctaagggc gaaaataaga ttggtacaac gattaaagga
     5281 atcggacctg cctatatgga taaagcagct cgtgtaggta tccgtattgc agacttatta
     5341 gataaagaaa tttttgaaga acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt
     5401 gtaaaaatgt ttgattcaga accacttgat tttgacgata tttttgaaga atattatgaa
     5461 tacggacaac aaatcaaaca atacgtcaca gatacttccg ttatcttgaa tgatgctttg
     5521 gatgcaggca aacgcgtgtt gtttgaaggc gcacaaggtg taatgctgga tatcgaccag
     5581 ggaacttatc cttttgttac ttcttctaac ccagtagccg gtggggtaac aatcggaagc
     5641 ggtgtagggc cttcaaaaat caataaagtt gtcggcgtct gcaaagccta tacgtcacgt
     5701 gtcggtgatg gaccatttcc aactgaatta tttgatgaaa caggagaaca aatccgcgaa
     5761 gtaggacgag aatacggtac gacaactggt cgtccgcgtc gtgtaggctg gtttgattct
     5821 gtagttatgc gccattctaa acgagtttca ggtattacca acctatcttt aaactcgatc
     5881 gatgtattaa gtggattgga aacagtaaaa atttgtacgg cttatgaatt agatggcgaa
     5941 ctaatctacc actatccagc aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa
     6001 gaattgccag gctggtcaga agacattaca ggatgcaaaa cacttgcaga actgccagaa
     6061 aacgcacgta actatgtacg tcgtatctca gagcttgttg gtgtacgtat ctcaacattt
     6121 tctgtcggac ctgatagaac acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa
     6181 ggataaaaac gacgactgaa tcaggacgtt cttaccagag cgagtcgaag tgtttatcta
     6241 tacgataata ataacttgaa cactgtttat tcagaataag ggactgtgac aaaaaccgtg
     6301 tcacagtccc tttttccgaa caaacggtgt tcaaaagctt gcatctacgg cactaagccc
     6361 aatcagatga aaaatatgga gtgctatttt tatctgattg ttcttactgc ttgatgcaga
     6421 gcagcttttt cacaacctcc tacatggtgt tcaaaatatt attcgccttt tgcttctttc
     6481 actaattttg cgaatgcttg ttgttggaat tcagcagcta aggctttcgt acgttgttct
     6541 tgctctgctt tgctcatttt tggattttca gtcattgctt ttaccatacc gtcttgaaca
     6601 taagcttttg cttcactttc catcgtactt tgctgtttct tcactaattc agctaacttg
     6661 gctgcttggt ccgtacttaa ttctacccat gtctctggga ttttgaatgt attcactcgg
     6721 ctgtcatatt cgtgattgtt atcagcaata ttgaaccaga atttatacat gtcgtttttg
     6781 tattcccaat aagtagtttt cgtcactttt tcagcatcgc cgtctgtctt ttctacttta
     6841 tttgtttcgt tatccgtacc agttgctttt ggtttttctt gtttttcatt tgttcggtaa
     6901 agatacactt tttcttttcc attccctaaa gctttataca aaagcatatc tgctccttgg
     6961 gaatcagcac tggaaacaat tttttctgtt tttgtttccg ttactttttc cattccaaag
     7021 tgattggaca aattagcgac gatcaaactt agtgaagcga cgaagattag tccgaaaatc
     7081 aaagataaaa aggtttgcca tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc
     7141 aagctgacaa tcaaaactcc aataatcatg cattatcccc cgctttcatt agaacaggtt
     7201 cttttttctt caataaaaag gcaataataa agccaagaat cccaaagatg atcgctacga
     7261 taaacgcggc atggtaacct gacagtgtgg cattgattgc ctgatcttta taagctaatg
     7321 gcgtattatc caatacagat ttgcttggga tatttgcttt tgtcacattc gtcaacacac
     7381 tgatcaaaat agctgtacca atcgagcttg cgatttgacg gaatgtatta tttaccgctg
     7441 tcccatgact gatcaagtta tttggcaatg cattcatacc agaagtcgtt accggcatca
     7501 tgaccatgga gatcccaaac atacggatcg cataaaggat cacgatatat aaaactggag
     7561 tcgtttttgt caagaaagca aatggtgcag ttgccgctgt caaaatgaac atccctgtga
     7621 tggctaaacg tttcgcgccg tatttatcga aaatcgcacc cgtcacaggc atcatagccc
     7681 ccatgatcaa tgcacccggc aacaacatca agccagaatg gaatgctgat tctccacgaa
     7741 tattttgaat atacaatggt acgaccattt ctgcccctac catcgccata tttgttacac
     7801 cagctaaaac agctgcaatc gtgaatgtag atgatttgaa tacacgaagt tccaagaatg
     7861 gatgttccaa atgtaattga cgccatgaga acaaggcgat gaaaatcaca ccaacaacta
     7921 agaaaccaat tactttcaag cttccccagc cgtcatctcc tacactagaa aatccgtaaa
     7981 gtaaactacc gaatccaatc gttgataaaa tagcggataa aatatcaatg ctaggatttg
     8041 acaatggaat aacacttttc attaggaaga atgctaaaac caagacaaga acaacgattg
     8101 gcagtaccat accaaacaag tcacgccaat ggtagttatc aatgatataa ccagataatg
     8161 tagggcctaa agcaggagca agtccaataa cgatccctga catccccatt gctgatcctc
     8221 ttttttctgg cgggaaaata gatagcatga tattttgcaa taatggcatc gatactccca
     8281 caccgcaagc ttggatcaaa cgaccgatta gtaatgtact aaaattaggt gctgtaaagc
     8341 aagtaatcgt tccgatcaaa aagatcgtca tggcagaaag atataaattt cttgaactga
     8401 atttattgat cagccatgct gttattggga tcatgatccc gttgactagc aagaaaccag
     8461 tcgttaacca ttgcacatca gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag
     8521 ctgtcgtcaa taaggtctga ttcaaaacgg tacaaaatgt accaattaat aagacaacaa
     8581 ctaaaagtgt ccgattatat ggttttccgt aaatatctac gggttggttg ttactcatat
     8641 ttctccttct ttctgaattt tttctgaaac tactagaata ctttagtcct aaattcgcta
     8701 tttgtcaacc tacttgacac ataagttaat acggatataa ttaagggaag caagaggaca
     8761 aaaggagtga caaaaatggt tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg
     8821 gttgttggca tcagtgaatt aagtgatatc gtaggtgtgt ctccacgtca attaaggtat
     8881 tgggaacaaa agggattcat tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat
     8941 cgtttaccta cagtcataaa agtagaactg atcaaaaaat atttagatga cggatttact
     9001 ttgtcaaaag ctgcagaaaa ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa
     9061 gttttttcaa aagcaatcaa agatatcgaa ttgatcgacg aacgctatac gattttacaa
     9121 atcggtaact tcaaagaaga gaatgctaag atgtacatca ttcatgacaa cgagacagac
     9181 gaattaactt acaagctcct tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa
     9241 gaactctaaa cgccactaat ctcctaagta ccaagctttt taataagtag atgctacata
     9301 aatacttgaa aaaaggcctg tttgaacata agaatatccg aacggtgatc tgcgtttctt
     9361 tcatatagaa aggaattctc catcactgtt cggatatttt tttatttttt tcggttactt
     9421 tcttctaata catctgttag ccaatttgca tattctgttt cccgattgat cgcatgcgct
     9481 aaaatcaggt agtgtccgta attttcatgt ttttcttttt cagtaggaaa gatcgttttt
     9541 tttcgctcta atagatgaag tcgtttttct tcatgaaggt cacgctgctg ctgaataatt
     9601 tctactaatg ccggatcatt cacgtctttg acgaaataca atttcagaac aaattcgtct
     9661 cgattaaccg gtagttcatt ggtgggtgta tgaatccact ctgccaattg ttccttccct
     9721 tcttctgtta attcataaac tttcttttcc aatttttcac ctgtgatctc tacttgatgc
     9781 tgaatgattc cttgctcttc catttttgca agttcaggat aaatctgact atgttttgct
     9841 tgccaaaatt ctcctatttc atttttgaac gattgattca attcatatcc tgtcatcttc
     9901 ttcttactta ataaccctaa caaaatatat cgaagtgtat tttttcttgc catcagtttt
     9961 ccctccttga caaatcttta aatccattct atcataaatg agtaatcgaa aacatgtagt
    10021 ttattacatg ttttaattta taaggagatg cttaaacatg aaagaattta aagaattgga
    10081 agattttatt ggaactcact ttatctatac gtatgacaat ggttgggaat atgaatggta
    10141 tgccaaaaat gaccatactg ttgattaccg tatccatggc ggaatggtca aaggccgttg
    10201 ggtaaaagac caagaagctg atatcgttaa attgacggac ggcgtattca aaatcacttg
    10261 gacagagcca acagggacag acgtggcatt ggactttatg ccaaatgaaa agaaacttca
    10321 cggaatgatc tttttcccta aatgggttga agaacatcca gaaatcactg ttactttcca
    10381 aaacgaacat atcgcagaaa tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt
    10441 acttgttcct gaatttgcta cgatcactta tatgggtgat gcaggtatag acaatgaaga
    10501 tgttattagc gaagaaccat acgatggaat gccagatgat atccgtagcg gaaaatactt
    10561 cgatgaaaat tataaacgag taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt
    10621 ctgacctgag tattagagaa agaaacggaa aaatagttcc tcatgttttg ccgttttttg
    10681 agctaatacc gagggtcagc tgattgaaca ccgtttatca agaggttgtg aaatcagcgt
    10741 tatgctttga gtaataagaa cgctagttag aaaatagctt ttcatatttt ctaggctagt
    10801 gggcttatta ccgagaagca gccgtttgaa caccgtttat caagaggttg tgaaatcagc
    10861 gttttgacct gagtattaga gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt
    10921 ttgagctaat accgagggtc agctgattga acaccgttta tcaaagattg taacaactgc
    10981 atattcacac agttaaatta gtgacttcaa aatgaaataa tgctcccagc aaaaaattag
    11041 gctgggagca ttatttcgtt attctgttca tcaaaaatca gcgttttgat tccatttcat
    11101 cacaagttcc tcctcattta ctgcttcatc tttttgctgt gtttgaattg taaaaccttg
    11161 tgaaagatag aaatgaatag ctgattgatt tttttgatag actgtcaaag tcagtgtttt
    11221 attggtttct tttatttttt tcattagttg cgtaccgatt ctttgttgcc ggtattccct
    11281 tttcacaaag attcctgcga taaactgatc aatcacccca gcaaaaccaa taatttcttg
    11341 atctatttct gctaagtaaa tcactgcatc tggtaaagct ttcctaacag attctacttg
    11401 attctcccaa taagttgatg gaatgaattc atgagaatcc aagttagcat gcagccaaat
    11461 cgatagaaca gattccagtt ctttggcaga caattttgtt gttgtgcgga tcattttttc
    11521 tctccttact tattcattga ttttaaaata aaaaccattt ttttgaagct cttttacaag
    11581 ttttcgcccc tcaaaaaagt atatcttctc ttgttttact tttctaatac caaaagaaac
    11641 agcaacttta ggatcatcag ctgaaacgat tcgataagtt ccaccaggca attgatccag
    11701 cttgactttc ccatgatggt cgctttgtac tgaaaacggc acaccatttc tagtaagtgt
    11761 ttttttctta tgtttcaaaa tgaatgaact accatttacc ggtttccctg acgaatcact
    11821 aataataaat gaaaaatcaa gcactttttt acttaatcta tactgataga ttagtcgtcc
    11881 gataaacact aacagtatga ctacagctat tatcgttcct gcaagaataa accattgccg
    11941 catggttctt tcctgatttc cttttccttc ttcttttttt accgattcgt tataaggaat
    12001 ccgatgtcct gtcactagca aacgatgcgt gttgatcata tacggcgtac atgtaagcaa
    12061 ggtaactaag tcttctcctt ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac
    12121 ttgtatattt ttaacttgat aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc
    12181 tccgttttct aaacgatcta attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc
    12241 agagatcact gaatgcgtcg aatttccacc aagcgggaaa gaggttcctt gcaagacgcc
    12301 tgctccattt tcaaggatcc taccattgag ccgatcataa agtgaaatat ttaattggat
    12361 cttaggtata ttgatacttc cgatcagcca ttcctctaat tggctggatg ttgtactttc
    12421 atttctgttc ttttcattga aaggatcagt accaggattg aatccttggt cagttaatcg
    12481 ttggtttttc tcctccattt tctttcgctg cacttcaatt tcagctgcgg tccgtttttg
    12541 tacttgttcg atacgttttt gatcaataaa actatttaaa gaatccacat aaaatggata
    12601 taaagcgatc agtaacccta cgacgataca tattaataaa aacaaacgaa acaaaagtcg
    12661 acgagactgt ttttgcttac tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc
    12721 tttcttttat tatatgggtt caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa
    12781 aaagctgtcc tgcatcaagt aataagtaca gcccaaaaaa atagctactc atattttttg
    12841 ttgggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt
    12901 gaaaactgcg ttatgctttg agtaataaga gcgctagtca gaaaatagct tctcatattt
    12961 tctaggctag tgagcttatt accgagaagc agctgattga acaccgttta ccgagaggtc
    13021 atgaaaaagc tactttgttt cgagtattaa gaaaaattct gtttcaaaac aaggagatgc
    13081 aacaaaactt atgtcgcatc ctctctcctt agaactttca cttatgtttt tcttgtaaaa
    13141 ttcataaata ttttcatgaa ttttctaatt tcttgcctac ctctcgcata ggatatatga
    13201 tgaatttatc taaagtttgc aaaggagttc gacaattgaa aaaatatggc ccatttttag
    13261 caattttttt cattgctttg gcatttctca tgcctcattt attcacacat aaactaattt
    13321 tgggtgctga tgccccattc cattacaatc ggttctatga tgccgcgcaa caaattaaat
    13381 atggggattt ccattatttt ccttcccttt atggattcca gcaaagtggc aggatcgtga
    13441 atgccgttta cggcccattt ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta
    13501 gctggtttca ttatcagatt ctttcaaatt tcattcttta tcttatttca ggattttcta
    13561 tgctgatttt actgagatat gtcaatgttc gaaattgggt aagtgtacca gtagccgttt
    13621 tattcatgag tacgtattct attcaatact ggtctatcaa ccaaggattc actagctggg
    13681 ggacttgttt ttatccttta tgtatgatcc ctattgctga tttcgtcctt cataaaaagt
    13741 tccctgttat caaagtcgct gtctcagtgg gacttatgac acagatccat ttactgtctt
    13801 cgatcatgtt agctttgatg tatatccctt tttatcttta ttactttttt aaccagactg
    13861 aaaaaggaaa agcactgctt caactgatta agtcggtctt gctttatttt gttctgacag
    13921 caaatatttg ggcaggatta gctattgttt acagtgggaa taagattctt cctccctttg
    13981 ttaacagaga aatgagtcaa aaggcgatag atctggatgg cagttattgg ctccattacc
    14041 ctaaggcctt tcctatattg atattatcag gttttttgct ttttattttc tttcaacaaa
    14101 agaacacctt attccaaaaa atcgtgttgt acacaagtct tgttttcttt tttctttcta
    14161 ctagcttgat tccatggacg acattgatcg aaaaaaatgt gccttttatt agtttgatcc
    14221 agtttccatt tcgatttttc gtgccattta cgctattgct tctactttac cttgcccttt
    14281 cactgaacca gtggtctgaa caaaagtggt tccgtctttt aagtatattt ggcattctta
    14341 ttttttcagc acagacgatc cagaacttat accagcatct ggaaaagtgg gaaaatgaaa
    14401 catttgtcag tcgacatact tatctattcg atacgccgga agaagcaagg aaaacattct
    14461 tcagcaaaga tatgtctacc agcttgttcg ttttccaaaa aaccacacct gactatctgc
    14521 caatctataa ggagacaaaa gacaataaat acgaccggta taacgaagaa atactagaaa
    14581 acgaaacaac ttttataaaa acacatccag gcggtctttt gacaatcaag tggaaaaaca
    14641 ctggggacaa aactgttcat atccctgtta tcgtctacga tcggactgtg ctacagcaaa
    14701 atggaaaaac attgactgat tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc
    14761 aaaaaggaat caacgagctc acgcttcatt atcaaacgcc aatctatttc tatttcattc
    14821 tttctcttac tttgattggc tggtttacct tgctatgttt atttatttat catagataca
    14881 agcttttacg tgcttaggca aaagggataa atacatccga tcaaaaaact ctccaaacgg
    14941 tataatcgtt tggagagttt tattttaatc agttttttta tgttatatgt tatatttttt
    15001 attcgttcac atctaattcg atttccaccc cgtagtgatc actgatgatt ggactatcga
    15061 tcccatcaaa aatccgttga tattttttaa cctgcattcc ttcagggacg aaaataaaat
    15121 ctatacgcaa tttttctgta ttttgttccc aaccatctat tttcttttcc acagtagctg
    15181 cttttccttt ttcattcgcg acaacatacg aatcttgaag aagaaggcaa ctttctctta
    15241 cagtataata accttgagag tctactggat tattgaaatc acctaggaaa aaaagtggta
    15301 cgcgagtttc caaaaagtag ttttccaagt ttttccactc aaaataaaag ccactttttt
    15361 tatctgtcca ccaagaaaag tggcagctcg agacaaaaat gtcctgatca tctatctttg
    15421 ttttcccaat caagatcttg cgtgtctgat aatcttcttt cttttgaaat tgggagacgg
    15481 taaggacctg actttccaat gtttttttcg taagcagtgc atttccttct tcatatttat
    15541 cataaccgat gtggctcatt tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa
    15601 gctgaactaa acggtaggca aaattatctt tacggatagg tacttgattc tttaccggac
    15661 agaacttcat tagcttcgca ggttctactt cttcagattc cagtaattgg ttgatttctt
    15721 gtaaagcaat cacttcataa tcttctaaca ggatcttatc tgctaattct tgcaattttt
    15781 tctctggttc tggctcaagc cagctatgcg tgttgagtgt catgaatttc attggttttc
    15841 tccttgtctt catttcgaaa caaaagggtt gtgacaaaat ttttgtcaca acctctccag
    15901 caaatagtgt tctcctgcta actcctcagt aataagctca atggaccagg agctattttt
    15961 tgtttggctg tttttatcac ttggtgcagg gcagcttttt cacgacctct ccactaaaca
    16021 gtattcaaac tgctgcttct cggtaataag cccaaccaaa cgaaaaatag gaggagctat
    16081 ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc tctccttaca
    16141 ttttaatgga gccgatcact ttaccagctg tcactgtacc taattgcttg atcactactt
    16201 cttctacttt atcgtttgta aatacgatca tgatcgttgg atctttttct tcgttttgga
    16261 tcacatcaag atccatctca gctaatttcg ttcctgttcc tacttcttgg ccttctttga
    16321 cagcaatctc aaaagctggc tgattcattt ggactgtatc gattcccata tggatcaaaa
    16381 cttcgatacc atctttcgtt tccagcccaa ttgcatgttt gctagggaaa atacttgtca
    16441 cttttccagc gatcggcgca acaacttctc ttgatgttgg aataacagca aagccgtcac
    16501 ccatcatttt ttgtgagaat accggatctt ttacttcaga taaaggaatc acttctccag
    16561 aagctgcgga aactagttct tgttctatac ctaagtattg cttactacct gcttgtccat
    16621 ttcctgtaga aaccatttct ggctctggaa tctctgctcc tgattgcagc agatcttcga
    16681 tatctgattt caagacatct gcttttggtc cataaactgc ttggactcca ttgtctttga
    16741 cgatcaatcc catcgcccct gctcgtttcc aggcttcttc agtccctact ttttctcgat
    16801 catttacact tacgcgtaac cgagtcatac aagcatcgac atctttgata ttttcttttc
    16861 ctcccagcaa atgaatgatc tggaccactt gctgatctgc tgttcctgtt ccgctgttag
    16921 atacaacggt ttcttcactg tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt
    16981 tgaatttttt gatcaagaaa tttgctaaga aatacgtaac aacaccaaaa gcgatcacac
    17041 acaacacgaa gttgataaga tctccaccta gacccgcttt gatagctaac ggtgtccgag
    17101 tcagcaattc gatattccca aatgaatgga cccgcaatgg caagatatct gccatcgcaa
    17161 atgcagcccc ttggatcact gaataaatga catataatgg cacagcagca aacataaaca
    17221 tgaactccaa tggttctgtt acacctgtta gaaagacagc caacgctgct gaaaaataca
    17281 ttgatttata tcttgctttc ttatcaggat cgacattacg atacatcgca aaagccattc
    17341 ccatcaagat acctgaagaa ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt
    17401 tatgcaacac aaactcatat tgcgacatat cccctgcacc tttaagattc actaaatcag
    17461 tggcccaagc caaccataac ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg
    17521 ataaaatctc atacgatccg ccaagctgtg tatagttgat aggaatcgtc agcatatgat
    17581 gcaatccaaa tggcaatagt aaacgttcta atgttccaaa taaaaacggt gctagaatcg
    17641 gtgcgctttc ttgtgattct gcaatccata aaccaaaatt attgattcca gcctgtacgt
    17701 ttggccacac cacagcaaga atcaatgcca cgattgttga ccataagata acaacaaacg
    17761 gaacaaagcg ctttccattg aaaaatgaca aagcatccgg caattttcta taattatagt
    17821 acttattata agccattgcc ccgacaaatc cagcgatgat ccctacgaat actcccatat
    17881 tcaaagctgg tgcttccaat acacttgtaa aaaatccttt aaccatgatc ttcgtaccga
    17941 acagagtgtg ggtaaatgca tcttcattcg caagcatgtc acttgtgaca ccaaagatag
    18001 ccccagtgat ccgattgatt aaaataaatg agatcccagc tgcaaatgct ccgcctgctc
    18061 tttcttttgc ccaacttcct cctattgcta aagcaaataa aatatgaaga ttaccaataa
    18121 ttgcccaacc aatattttca attactccgc ccgttgtgac aagcggcgtc caatcaggat
    18181 tgatcaaggg aattgatttc cctatactga tcatcaaccc agctgccggc ataactgcta
    18241 ccacaaccat taaagccttt ccaaattttt gccaaaactc aaaactaaat atctttttca
    18301 ttccgtttag ctcctttacg caatcggttt cgttaaacgt attatagcgt gcgttttctt
    18361 ttttgtaaat gctttatttt ttatttttct gtctatacca ttctataaaa cctataatac
    18421 gtgtgttccc ttctataatt ttctcctaaa aatttatttt tttaagcttt tttctatgaa
    18481 atattttctt ttgtctattg acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac
    18541 agctcattag gaggagaaaa atgaaacaga tcaaacgact atttgctatt gatccatgga
    18601 aaatcagcac gacacaatta gataaaaaaa atcttcgact gcaagaatct ttaacaagta
    18661 ttggaaacgg ttatatgggg atgcgcggga actttgaaga aacttactca ggggaccacc
    18721 atcaaggtac ttatttggcg ggtgtctggt atcccgataa gacacgtgtc ggctggtgga
    18781 aaaatggtta tccagaatac tttggaaaag tgatcaacgc cttgaacttt atcgcgatag
    18841 atatttcgat caacgatcag ctgatcgact tggcttcttt ggaaccagaa gacttttact
    18901 gggaattaga tatgaaaaat ggtgtattat ctcgtacatt cacaatcact acttctacta
    18961 ataaagtacg catttccttc gaacgttttc tgagcattgt caaaaaagaa tcagcttata
    19021 tctatttaac tatagaaatg cttgaaggta gcggaacagt caaactcgta tcaaaattgg
    19081 atggaaatgt tcaaaacgaa gacagcaact atgatgaaca tttttggaat gagattgatc
    19141 gtggaactat agaagaaatc ggctatttga cgactaaaac tattccaaac aactttggga
    19201 ttgaacaatt tactgttact gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc
    19261 ctgactatca cgaagatact ttagcattac atggttgctt gtctgcagaa ttatctcaag
    19321 gtgagacatg gtcagtggaa aaagaaatta tcgttcttac tagtagagat attccagaag
    19381 cgcagcaagc agaaaaagct gtctcagaat taacgaacca gacaaacaat tatatccaag
    19441 caaaagaaga gcaaacagcg gcttgggcag aacgatggaa gcttgccgat gtggtcatcg
    19501 aaggagacga agaagcacaa caaggtattc gattcaattt gttccaacta ttctctactt
    19561 actacggcga agatgaacga ttgaatatcg gccccaaagg attcacagga gaaaaatacg
    19621 gcggagccac ttactgggat acagaagcgt atgctgtccc tctttatctt gctttagcca
    19681 aacctgaagt aactaagaat ctgctgaaat atcggcataa tcaactgccg caagcaatcc
    19741 acaacgctca gcaacaagga ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg
    19801 tcgaatgcca taacgagtgg gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg
    19861 cctatgcaat ctacaattat acgaattaca ccggcgatac ggaatatttg aaaaatgaag
    19921 gactggaagt tttagcagaa atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac
    19981 gagcaaataa atacatgatc cacggtgtga ccggcccaaa cgaatatgag aacaatatca
    20041 ataacaattg gtatacaaat acgatcgcag cttgggtctt gcgttacacg cgagaaaatt
    20101 atttaactta tcaaaaagaa gctgctatca ctatcagcga agaagaacta gccaaatgga
    20161 aagatatcgc agagaacatg tacttcccag ttgacgaaga attaggaatc tttgtccagc
    20221 atgacacatt cttagataaa gatctcatgc ctgtatccga tattcccaca agcgatcttc
    20281 ctctgaatca gcattggtct tgggacaaaa tccttagatc ttgcttcatc aagcaggcag
    20341 atgtactaca ggggatttat ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa
    20401 actttgattt ctatgaacca atgactgtcc atgagtcttc cctttcgcca agtatccatt
    20461 cgattttagc agcagaatta ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag
    20521 ctcgcttgga tttggataat tacaataacg acacagaaga cggcttgcac atcacttcca
    20581 tgactggtag ctggttgtcg attgtccaag gatttgctca aatgaaaact gcccatgatc
    20641 agttgagatt cgcaccattt ctgccaaaaa cttggaaagc ttattctttc catatcaatt
    20701 atcgcggacg tttattgttc atctcagtca cagatgaaca agttactttc aagttattag
    20761 aaggttctcc tctagaaatt tacgtctatg aacagtctta ttctttagcg gatcgtctag
    20821 aaataacgct aagaaaggag gaagcaaatg tttaaagggg tattgtttga tttagacggt
    20881 gtcattaccg atactgctga attccattat cgtgcttggc gaaagcttgg tcaggaaatc
    20941 ggcattacga tcgaccacac attcaacgaa cagctaaaag gcgtcagccg agaagactca
    21001 ctctctttgc ttttagctta tggcggaaaa gaacactctt ttacaaagga agaatttgca
    21061 gaattagcga aaagaaaaaa cgattattat ttagagatga tccagacgat cgaaccaaaa
    21121 gatgtcttcc ccggggtcgt gcctttactg gattctttaa aagaagaaaa aatcaagatt
    21181 gctcttgctt ctgcaagtaa aaacgggcca ttcctacttg aaaaaatggg tcttacacct
    21241 tactttgatg ctattgccga cccggcagaa gcaacaaacg gaaaacctgc gccagatatc
    21301 ttcctccttg cagcaaaagc tgtcggacta aaagcagaag aatgtatcgg gatcgaagac
    21361 gcgcaagccg gtatccaagc catcctctca agtggcgctc aaccaattgg cgtgggacgt
    21421 gctgaagacc ttggagagga gatcccgctt gtccctgata cgacttttct tacgatcgat
    21481 tacttgaaag aaaagtggca tgaccatgga taatccagag atctccgtcg taccagttcc
    21541 agagactgaa ataaagaaaa tcacattgaa gaacgactac ttagaagtcg ttcttctcaa
    21601 ttacggtgcc cgcctgcacc aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt
    21661 tttattgtct tacgatcgtt tcgaagatgt tctaacagac aaaagctttt ttggagcaac
    21721 tgtcggacct gtagccggaa ggatccggga tgcttcttgg ggtgagcata gattggagaa
    21781 aaactgcgga tcacaccata tccacggtgg cacgaaaggc tggtctttcc aattttggga
    21841 agtagaagtt ttcaaaactc catcatcaat aggtgtggtc ttttacttaa aagatgaatt
    21901 ttctacctat ccaggaccaa tcactgctac gatcacttac cgtttaacaa aaaacgaatt
    21961 agagatgaca acctctgcta gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc
    22021 ctacttcaac ctttcaggaa atggaacaag agacatctat gaacatcagt taacggtctt
    22081 tttagatggt gtgcttgaat tagatcaaga aaaactgcct actggaaatt ggacaaaaaa
    22141 agaaaaactg ccaatcgact ttagaaaatc cccaacactg caagagatcc ttgcatgtta
    22201 tccagatgga ctagatgatg tatttctttt gcatcatcct aggctttcca aaacttcctt
    22261 acaattattc gagaagcatt ctggtcgcca aatgactata gcaacttcta ataaaagtat
    22321 ggtattattt tctacgactg gattcgaagc tgatttctct gtcaatggaa aacagatgca
    22381 ttcaaactac ggattagcaa tcgaaccgca agaaatccct gatatcgttc atttccccaa
    22441 atttggatcg atcaacctcc atcctggtca agaacggatc agtcagacga tttatcggtt
    22501 ctctgcacaa taacaagaga aaggtgtttt tatcatggct attaccgtaa aagatgtggc
    22561 gaaaaaagcc ggtgttgcca cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat
    22621 ttccgagaca acaaagaaga aagtccgaaa aattatggat gaactaggtt atgttcctaa
    22681 catgactgcc cgcaatctag gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc
    22741 tcttgattca aaagagcgga tcggtaaccc attttatctt gagatcatgg aagcaatcaa
    22801 cgaagaagcg cggaattatg atatgactac agcaatcgct actgctaaaa attttgaatt
    22861 gctgttagag aacgtccagc gtatgcattt acaaaaacaa gtagatggtt ttattctagt
    22921 gtattcagat agtcaagata ctgtcattga ttatttatac caaaaccaca tccctttcac
    22981 tttgatcgga cagccttatc atcatgagaa cgaaatcgtt tatgtagata atgataacca
    23041 gcttctaggt aaacaagcaa cagaattttt gatccagaat ggacataaga atatcctgtt
    23101 tgtgacaaac acgactcatg aaagtttgta tttcgaacga tatttcggtt atcaaaaagc
    23161 aatgatgatg gctggtcttc ctgcgttatc cagtgtagta ctagagcatc cggaggatta
    23221 tctgtccttt gagaaaacgt tgaaagaaaa cgaagccaca gcattggtgg tcattgacga
    23281 tatcttcgcc cttcgcacga tgcagttggc gcaaatgtat ggctatgtca ttccagagac
    23341 actctccgtc attagcttca acaactcgat cttctcaaca ttaacacacc cttaccttac
    23401 aagtatcgat atcgatatct ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt
    23461 gatccatgaa gaattgagca gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag
    23521 agagacagtc gttcctttag aaaacccata ctaaatagct gactcactaa atacgctgac
    23581 catttatcct atggtcagct ttttatgtaa aagaagatac tacttctaac acattgttta
    23641 tctgtaaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    23701 tataaacggc ggaagcagaa gcaactcctt cggaaataag ctgaaattca caaaaattta
    23761 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
    23821 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
    23881 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
    23941 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
    24001 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
    24061 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
    24121 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
    24181 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
    24241 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
    24301 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
    24361 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
    24421 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc caaataaaat
    24481 aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt tttggatcat
    24541 ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg tataaaaaat
    24601 caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat gttcgaatag
    24661 attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg agcagttatt
    24721 ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga atccaagtaa
    24781 atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg aactgccgac
    24841 agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac
    24901 aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc atgaagcaac
    24961 taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga ttcctttgat
    25021 gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa acaacaaaca
    25081 catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga tcagtactcc
    25141 taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa tatttttatt
    25201 catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaaacta ttcacttggc
    25261 aggacctcct actaaatcag cgacaaaacg tcggctgaaa gacttatttt tttatttttc
    25321 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
    25381 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
    25441 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
    25501 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
    25561 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
    25621 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
    25681 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
    25741 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
    25801 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
    25861 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
    25921 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
    25981 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
    26041 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
    26101 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
    26161 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
    26221 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
    26281 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
    26341 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
    26401 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
    26461 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
    26521 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
    26581 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
    26641 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
    26701 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
    26761 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
    26821 gaggggattc tttgagttta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
    26881 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
    26941 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
    27001 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
    27061 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
    27121 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
    27181 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
    27241 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
    27301 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
    27361 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
    27421 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
    27481 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
    27541 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
    27601 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
    27661 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
    27721 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
    27781 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
    27841 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
    27901 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
    27961 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
    28021 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
    28081 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
    28141 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
    28201 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
    28261 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
    28321 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
    28381 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
    28441 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
    28501 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
    28561 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
    28621 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
    28681 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
    28741 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
    28801 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
    28861 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
    28921 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
    28981 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
    29041 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
    29101 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
    29161 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
    29221 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
    29281 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
    29341 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
    29401 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
    29461 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
    29521 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
    29581 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
    29641 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
    29701 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
    29761 acgcttctgc ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
    29821 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
    29881 agtcgataga ttgcaggatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
    29941 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
    30001 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
    30061 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
    30121 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
    30181 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
    30241 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
    30301 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
    30361 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
    30421 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
    30481 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
    30541 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
    30601 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
    30661 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
    30721 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
    30781 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
    30841 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
    30901 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
    30961 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
    31021 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
    31081 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
    31141 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
    31201 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
    31261 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
    31321 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
    31381 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
    31441 aaatgatttt gttttagtta atttgaatga tttgatccaa aaagacgaac aaggaaatct
    31501 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
    31561 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
    31621 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
    31681 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
    31741 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
    31801 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
    31861 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
    31921 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
    31981 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
    32041 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
    32101 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
    32161 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
    32221 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
    32281 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
    32341 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
    32401 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
    32461 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
    32521 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
    32581 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
    32641 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
    32701 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
    32761 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
    32821 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
    32881 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
    32941 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
    33001 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt
    33061 atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt
    33121 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
    33181 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
    33241 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
    33301 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
    33361 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
    33421 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
    33481 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
    33541 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
    33601 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
    33661 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
    33721 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    33781 cgagcgacta gcggataaag ttgtgatcat acgcgaagga acactcgatg aagaaggaac
    33841 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    33901 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    33961 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    34021 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    34081 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    34141 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    34201 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    34261 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    34321 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    34381 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    34441 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    34501 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agttgtacga tgattttttt
    34561 ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
    34621 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    34681 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    34741 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
    34801 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    34861 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    34921 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    34981 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    35041 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    35101 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
    35161 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    35221 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    35281 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    35341 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    35401 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    35461 cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact
    35521 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    35581 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    35641 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    35701 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    35761 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    35821 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    35881 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    35941 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    36001 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    36061 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    36121 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    36181 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    36241 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    36301 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    36361 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    36421 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    36481 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    36541 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    36601 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    36661 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    36721 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    36781 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    36841 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    36901 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    36961 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    37021 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    37081 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    37141 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    37201 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    37261 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    37321 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    37381 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    37441 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    37501 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    37561 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    37621 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    37681 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    37741 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    37801 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    37861 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    37921 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    37981 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    38041 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    38101 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    38161 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    38221 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    38281 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    38341 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    38401 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    38461 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    38521 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    38581 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    38641 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    38701 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    38761 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    38821 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg
    38881 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    38941 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    39001 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    39061 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    39121 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    39181 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    39241 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    39301 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    39361 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    39421 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    39481 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    39541 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    39601 attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct
    39661 tcttgaagtt tgttatactt taatt
//
LOCUS       DACCCM010000017        36561 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCCM010000017 DACCCM010000000
VERSION     DACCCM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.017"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            99..746
                     /locus_tag="IVK78_000906"
     CDS             99..746
                     /locus_tag="IVK78_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6374393.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVK78_000907"
     CDS             1139..3784
                     /locus_tag="IVK78_000907"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP6374394.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVK78_000908"
     CDS             4094..5407
                     /locus_tag="IVK78_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP6374395.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVK78_000909"
     CDS             5397..6050
                     /locus_tag="IVK78_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6374396.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IVK78_000910"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IVK78_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP6374397.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IVK78_000911"
     CDS             complement(6671..8023)
                     /locus_tag="IVK78_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6374398.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLAEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="IVK78_000912"
     CDS             complement(8089..9378)
                     /locus_tag="IVK78_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6374399.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="IVK78_000913"
     CDS             complement(9465..10169)
                     /locus_tag="IVK78_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP6374400.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="IVK78_000914"
     CDS             10369..10635
                     /locus_tag="IVK78_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6374401.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10803..11033)
                     /locus_tag="IVK78_000915"
     CDS             complement(10803..11033)
                     /locus_tag="IVK78_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6374402.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="IVK78_000916"
     CDS             11105..11260
                     /locus_tag="IVK78_000916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374403.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11569..11793)
                     /locus_tag="IVK78_000917"
     CDS             complement(11569..11793)
                     /locus_tag="IVK78_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6374404.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="IVK78_000918"
     CDS             12060..12395
                     /locus_tag="IVK78_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374405.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="IVK78_000919"
     CDS             12628..13569
                     /locus_tag="IVK78_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP6374406.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13571..14461
                     /locus_tag="IVK78_000920"
     CDS             13571..14461
                     /locus_tag="IVK78_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP6374407.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="IVK78_000921"
     CDS             14504..15304
                     /locus_tag="IVK78_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6374408.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="IVK78_000922"
     CDS             15319..16170
                     /locus_tag="IVK78_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6374409.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16167..16526
                     /locus_tag="IVK78_000923"
     CDS             16167..16526
                     /locus_tag="IVK78_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP6374410.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16541..16702
                     /locus_tag="IVK78_000924"
     CDS             16541..16702
                     /locus_tag="IVK78_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6374411.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16699..17025
                     /locus_tag="IVK78_000925"
     CDS             16699..17025
                     /locus_tag="IVK78_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374412.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17022..17837
                     /locus_tag="IVK78_000926"
     CDS             17022..17837
                     /locus_tag="IVK78_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP6374413.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17976..18287
                     /locus_tag="IVK78_000927"
     CDS             17976..18287
                     /locus_tag="IVK78_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374414.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18284..18514
                     /locus_tag="IVK78_000928"
     CDS             18284..18514
                     /locus_tag="IVK78_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374415.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            18521..18724
                     /locus_tag="IVK78_000929"
     CDS             18521..18724
                     /locus_tag="IVK78_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP6374416.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18721..19020
                     /locus_tag="IVK78_000930"
     CDS             18721..19020
                     /locus_tag="IVK78_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374417.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            19263..19676
                     /locus_tag="IVK78_000931"
     CDS             19263..19676
                     /locus_tag="IVK78_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6374418.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            19689..20075
                     /locus_tag="IVK78_000932"
     CDS             19689..20075
                     /locus_tag="IVK78_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374419.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            20626..20850
                     /locus_tag="IVK78_000933"
     CDS             20626..20850
                     /locus_tag="IVK78_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374420.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            20868..21296
                     /locus_tag="IVK78_000934"
     CDS             20868..21296
                     /locus_tag="IVK78_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374421.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            21274..22692
                     /gene="terL"
                     /locus_tag="IVK78_000935"
     CDS             21274..22692
                     /gene="terL"
                     /locus_tag="IVK78_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAP6374422.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            22704..24239
                     /locus_tag="IVK78_000936"
     CDS             22704..24239
                     /locus_tag="IVK78_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6374423.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            24319..25197
                     /locus_tag="IVK78_000937"
     CDS             24319..25197
                     /locus_tag="IVK78_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP6374424.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            25199..25516
                     /locus_tag="IVK78_000938"
     CDS             25199..25516
                     /locus_tag="IVK78_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374425.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            25565..26266
                     /locus_tag="IVK78_000939"
     CDS             25565..26266
                     /locus_tag="IVK78_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP6374426.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            26281..27171
                     /locus_tag="IVK78_000940"
     CDS             26281..27171
                     /locus_tag="IVK78_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP6374427.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            27194..27409
                     /locus_tag="IVK78_000941"
     CDS             27194..27409
                     /locus_tag="IVK78_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374428.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            27421..27762
                     /locus_tag="IVK78_000942"
     CDS             27421..27762
                     /locus_tag="IVK78_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374429.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            27762..28133
                     /locus_tag="IVK78_000943"
     CDS             27762..28133
                     /locus_tag="IVK78_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374430.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            28126..28521
                     /locus_tag="IVK78_000944"
     CDS             28126..28521
                     /locus_tag="IVK78_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6374431.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            28523..28900
                     /locus_tag="IVK78_000945"
     CDS             28523..28900
                     /locus_tag="IVK78_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374432.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            28901..29509
                     /locus_tag="IVK78_000946"
     CDS             28901..29509
                     /locus_tag="IVK78_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6374433.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            29509..29850
                     /locus_tag="IVK78_000947"
     CDS             29509..29850
                     /locus_tag="IVK78_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374434.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            30092..32881
                     /locus_tag="IVK78_000948"
     CDS             30092..32881
                     /locus_tag="IVK78_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP6374435.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNGGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVASSMESFLKVIAANIPRAVSFLSNLFDAVQKFGSALKFMMPFLVPA
                     TTAFGALMFQLKGIPTIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIVGAVVGAI
                     AVFGYFMATNEEVRNKVISVWNDVKDSVLGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            32871..33611
                     /locus_tag="IVK78_000949"
     CDS             32871..33611
                     /locus_tag="IVK78_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6374436.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            33608..36319
                     /locus_tag="IVK78_000950"
     CDS             33608..36319
                     /locus_tag="IVK78_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6374437.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            36332..>36561
                     /locus_tag="IVK78_000951"
     CDS             36332..>36561
                     /locus_tag="IVK78_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP6374438.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT        12655 a         5655 c         7573 g        10678 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tccgctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgt gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaagc ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga caacgagatc
    13381 atcagtgagc taggtaaacg tgaagccagt atcggtatca cacaaagaaa cattatcagc
    13441 tggaaacttg ttcgtacaag acggatgaac acgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaaggag
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
    16201 gaggtatcaa gtttgattct atcgcggaag cagagtatta cgatctagcc ttgtggcaag
    16261 ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg
    16321 aactagaggg caagaagtat cgcaagatcg agtatattcc cgacttcaca ttttacaaaa
    16381 gcggcaagct ggtcaaagtc gtagatgtta aggggatgca gacaaaagac tttaagatca
    16441 aggcaaagtt gttctgtcat caatatcaag tgccgttgat attagccaaa aaatatcgga
    16501 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
    16561 tcaaatgcga attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt
    16621 gatggtcaat gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga
    16681 aatccgtaga ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg
    16741 ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta
    16801 ggaaagattg ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg
    16861 aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg
    16921 tctgcagaaa ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc
    16981 gatcgaaaag ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa
    17041 tcaaattggg tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt
    17101 tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca
    17161 aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc
    17221 caattcgctt gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa
    17281 agacaacgat tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt
    17341 acaaggaatt tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc
    17401 ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct gcttgatgtc ctttcaggag
    17461 attcctctaa agttgaagct gaggtattaa cgaactttgc taaatcagtt gaatctaaag
    17521 ggtttgaagt cgaagacgta acacttggtg ttccagatgt cgataaagaa acacaaaaat
    17581 caatcgatgc gatcattcga gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa
    17641 ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac aaaagctgca gaagcagagg
    17701 cagaatctaa tcgcaaagtc gccgaatcag taacagacaa tttgattcgt tatgaagaag
    17761 ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg agcagatact gtagttacgg
    17821 atgaagcagg caaataatat gggattcttt atggctaaaa ttctcttgtt cttaggttta
    17881 gtaggagcag catatctcgt gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac
    17941 aacaacgatg aaatgaaatt ttaggaggag aaataatgga cgaactaatc acaaaagtag
    18001 agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag tttttgaaag
    18061 tagctgagga attcggggaa atagcttcgg cgatggcaag aagtaatgat gaactattta
    18121 aagatagcgt aggagacgtt atcgtcacgc tgattatcct ttccatgcaa aaagggacaa
    18181 acgtacaaga gtgtttagaa atggcataca acgaaatcaa aggacgcaca gggaaaatgg
    18241 tagatggtgt attcgtgaag tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc
    18301 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaaca tagaacttcc
    18361 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
    18421 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttaaaag aatttcgatc
    18481 aagtatagac gacactttca gattagggag ttgattgaca atgaacaaca gacatcgcag
    18541 aataacaaaa ctaagaaaac aggaactgaa tgtactaaag acaaagtttg aaaaagaata
    18601 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcaagtga
    18661 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaatgag
    18721 atgaaactaa aagacggatt ttacgctagt agtcacggca tcggcggttt aatgctagat
    18781 atgccgacaa agaaccctaa aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtt
    18841 cgctgtgaag cagaagagtt catttatccg tttcgtggat atgtagaaca cgtctataat
    18901 cactcagcga tcattcgtat tgaaaacaca atggaatgcg ataagtggtt agcgaaaagc
    18961 aaagagaatt tagctgtagc gagattggtg gatatggagg ttataaacaa tgaagtttaa
    19021 aatctttgaa gaggacactc gttataaatt agaaaaagaa ttaaacgatt ttgcgaaaaa
    19081 caatgagatt cagcatatat ctttagcaac ttctaagaga ggttatgcaa attactatgc
    19141 agctgttgtg agctatgtaa gtcgagaagt gtaactcggc aaataaaaaa agccggatcg
    19201 ctccgactga ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact
    19261 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
    19321 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
    19381 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
    19441 ttcaaatgat ggatatagaa gcggagagag acgcgattct agtggctttg atggctctta
    19501 gtctgattag tcgtcagata ctctactaca gcttctgtgt gccagacagc ttctcaaact
    19561 acagaattag ccgtgaagtg ggttattcag aaagaagtat acaacggatg aaatcggaag
    19621 ctctaataga gtttgcagaa gcatataaac acggaagaat aattgcttat aaataggggg
    19681 accatataat gtggaaagat tatgtatcgt tgaaagagtt gaaaaaagat cttgttttca
    19741 aaagaattgt agaatggtca gagagtgaat taattttaga agatggaacc aaaatggaag
    19801 ttgtatgtag cgaatcagat tgctgcgcat gggccgaagg tgaattcaaa aatgtaaagc
    19861 ttgatgcagt gattacagat ataaagattt ttgataaagg taaccgtctc tataatggtg
    19921 acggacatac ctcttatgct gaagtcgttg tttatcataa tcgtaatgag atcgctaaag
    19981 cagaatgcac agcgaatgat gggaatggcg gctactatta tagtgtttgt gctttaaagg
    20041 tcaaagacaa actttgtata gtgactgacg cataaaaaaa tggcggtttt ttggcggaat
    20101 gatggcggtt tttagccatt tatcagtgat attatggtag tgtcgaaaga ttagtgatag
    20161 gtctgagaca aaataataat aaaaggaaca tcgttttatt attgtttcac aattaagctt
    20221 cgatagacag cagcggaaat attaagaata aggatgtgaa ttttaactcc ttctaaattg
    20281 ttcttattat ctatcatccg ttgctgtcta ttgttacata cagatcgctt aggcggtctt
    20341 tttattttgg ccgaaaacct acattttcga tggccgatta ttggaggagg aatagcatgt
    20401 tcaaactatc tgaaatcatt aagaagtccg acgttgagaa attagagatg ttaaaaaaga
    20461 agttgaagaa atagcagtct cttcgtgagg ctgttttatt ttgctcacaa aaatagacca
    20521 ctatcgggta atagtggtca ggaattaaat gaaaaagatg ttagggttgt tagctaagta
    20581 taacatcata acgcttacaa aacaatgcaa gaaggataaa ttattatgag aacttactgg
    20641 tatgtgtcat taaacaataa atatccgaag ccaatgaaag gacagcatag gcgtgtagtc
    20701 atgtctgttc aaatgaaggc gaagtattcg attgtagaaa tgatcagaga ggcaacgcca
    20761 gtagagattg attattgcaa gctagtctat tgtgggtgcg gccgttggaa agaggatcat
    20821 gtacaaaaaa atatcagcaa gtacatatag agaggcggtg attaaggatg gaaaaaagcg
    20881 atacgaaagc gaacagatac aaacctactg ctgcagagaa aaaactcttg gaagtattga
    20941 taaatccaga taacttaggc aaaccggtgc aagagttgtg ttcactggcg aaaataggta
    21001 ggaccaaata ttacgacgcc atgagcaaag atggctttgt tgatctagtt aacgaaacaa
    21061 caatggatct gatcaaagga aaagcttctg atgtccttaa tgccacttac aagtatgcat
    21121 tgactgaaaa aggtcatcag gataggaaaa tgattctgac tattgctgga atctatgcag
    21181 acaaacaaga actcaaacat tctggcggcg tcgatatccg aaaacagtat gagaaaatgt
    21241 ccgatgaaga attagaagag ttggtgaaac ggtatgaaaa aatcaacgac tcttaatcgt
    21301 gttgagtatg ccgttgttca aagagagcta gccattcgaa aagctaggaa aagtttttgg
    21361 ctattctgcc agcttcttta tccagagttt tacaaagatg atcggttgta tttgaaagat
    21421 ttatgtcata cgctacaaga tttttacgaa gataaaatcg acaaaaaaat tttggttatt
    21481 aacatgccgc ctcgacatgg gaagactttt acagcgcggc tattcgtttt atggatgttt
    21541 ggacaaaatc caaagacgaa aattattacc ggatcttata accaaatcct gtcatcttta
    21601 tttgcgcaac aaacacgtga tggaatcatg gttgaaaacg aaaatgttaa ggctgaatac
    21661 tttaaagata tttttccaga ggtgcaaatc aaatatggag atgctgcaaa gggtttctgg
    21721 tctttagatg gttcagaaga aaagaattat ttagcgactt ctcctggtgg aacatccact
    21781 ggtattggcg cgaattatgt aattgttgat gacatcatta aaaccgcaga agaagcctat
    21841 aacgagcgtg ttttagattc tcactgggaa tggtataaca acacgttggc ccaacgaatg
    21901 gaacggccac gtaaacagat attgattatg acacgttggt caagtaatga tttagccgga
    21961 aaaatgttga cgagacgaaa aaataatgtt catcagatct gttataaagc agtacaagaa
    22021 gacggctcga tgttgtgcaa cgagattatg acccacgatg agtacttaga tgtggtacaa
    22081 gaaatgaatg tagatattgc agaagccaac tatcagcaag aaccaattga ccaaaaaggc
    22141 aggctatatc agaagtttct tacttatgat acgttgcctg ataacattat caaaatatgg
    22201 aactacaccg acaccgccga caagggtgcc gattactttg cgtctcctgt ttttgctgag
    22261 acgtctgatc atgatgctta tattatcgat gtcatgtata caaaagagcc gatggaaaca
    22321 actgaaatag cccacgctaa tatgattatt cgcaatcgag ttaataaggt acggattgaa
    22381 ggaaacaatg gcgggcgtgg attcagaaga aattcagaaa gaattacaaa agagcgaggc
    22441 tatcgaggtg cagtatttga agactttcat caatcggata ataaactgtc tagaatactg
    22501 gcgaatagtg cttgggtgga aaatcatgtt tactatccag atgattgggc gacacgttgg
    22561 ccggactttt atgaggctat ggttagttat caacgtgaag gtaagaacgc tcatgatgat
    22621 gctccggatg cagttacagg aattgcagaa acgattatag aagcagatga cttcgagttt
    22681 attgcttatt aggaggtggg tctttgtttt attctggaat cacattgaaa aaatacaaac
    22741 gtttaagaac gaaatattca acgcaaatcg aagaaggatt attcgatcct aacgctttta
    22801 tggaagatat gaaacccttt ttcgatgatc gagaaagaaa gtatcttgct tatactagcg
    22861 agaaaaacga gatcgacagt cgtgagaaac caaaaacaaa aatcattaag gtcaataaca
    22921 agattcatgc gggaatgtat tcagtggtcg ttgatcaagc agttaaccat ttcacaggaa
    22981 taccaattaa atgggattat gatgtttcgg aacagaaaag gacattcatt caacgaatga
    23041 gagacaaatt actgggtaat gatatcagcg ccccttctac cccagaagaa tttgaaacac
    23101 tcacgagaca attaagtact atgcggtttg gaatgttaga ccccgatact gcaacatttc
    23161 aaggagcgac agggcttgct ttcagattac tggagcctgt tgagacaaat gatggttggg
    23221 aactaagagc aagcaacatt gagccgtgga aagctgaaaa gtacgaaaac ggcgcagtgt
    23281 acattaaaga gaaatacgat ccatatcaaa agaagtttta tgaagagatg aaggttgtca
    23341 cgccgaaatc aatatttact tatgctcgat atactgatta ccctggcacg gtacaaggag
    23401 catttaaaac gctgggtaat gttcctaatc ctttaggcgt tatcaatctt acggagttta
    23461 aaaacaatac aaaccgttac tgtgactttg aagttgcaga ggagattagt gatgctattg
    23521 atcgtgtttt atctgatcag cagaacgaga ttgaacaatt caagttagct tatatgctga
    23581 tcactggggc agatttgtcc gaggataaag ccaagaagat gatggagcaa ctagggatca
    23641 tcaatctgaa agaccctaat gcgaaagccg gctacgtgac aaaggatcta caaaaggatt
    23701 tcaacgagta tcattttgat cttcttaaaa agcaattcta cacggtgtgt aaagcaattg
    23761 atttcaatga cgaagtcttt aagtcgaata gttctgggga agcccgaaag tggcaaatca
    23821 ttagtttaga agctaagaca aataccaaag aacagtactt tcgagaagga ttgaaagagt
    23881 gtgccgaggt tatggctgct tatttggatt ttgccgagaa agttacaatt gatccggaaa
    23941 agatcatttt caccttctct cgttcgcttc caacagattt aagctaccta gctgaggcat
    24001 tgcctaagtt ggcaccatac gtttctaaga ggacgattca aagtcagatc ccgttcgtca
    24061 cagatgttga ttatgaaaac gaaatgattg acgtcgaaag cagacaatct tacccagacg
    24121 gcgagtatgg aggtggcatc gttgagtcaa atagaaacga agtactggac caaacgtcga
    24181 gaactggaag atttagcaag actcaaaatg gaaaatcaaa cgctaaagaa actaagtagt
    24241 gtctttcctg atgcgttgaa agaaattcaa gcggagttat tatcccaagc tgatttacac
    24301 gatattacct atgctgagat gatggaattt tatagtacaa gtgatcagaa aaaatatcgt
    24361 gaatacgttg aaaagaacta caaatcactg aagatgtatg atgcaaaata caaagagttc
    24421 atcgaagaat actttccttc tttcgactac gccaaagtga atcggctgct tcaaatgagg
    24481 tcagatattt ttcgaattct tgcagagagt gcaatagatg cagatgtgaa taaatatttt
    24541 tcggatcggt tagaagatat cttgcaacgt tcgtatgtat caaacgcaaa cgtatttgcc
    24601 cagattttga acgtagattt gccgaattac ttatcaaagg aagaacttga acgttacctg
    24661 aactatcctt ggtcaggtaa gacattctcg cgtcgtttat ggggcaatat ttcatctcta
    24721 gaacaaaagc tatctaacgc cattacaact agtgtagcaa gtggcgaagg agttctcgtg
    24781 gcattacgaa caatgcgaga agacccagaa atatgcgata tgttcaaaag ggaggaatcg
    24841 aagtacaaca aggcgatcga aaatcttgtg agaacagaat acgctaaatt cgctcaagat
    24901 ggtattgaga aatcttatat tgaaactggc gtagacgaat acaatgtctt aactgcaaaa
    24961 gatgaaaagg tatgttcaat atgcggtggc aaagcaaaga acaacccata caagttgagt
    25021 gaagcgatca ttggtgagaa ccgtgcgcct tttcatggcc gatgcagatg tacagatgtt
    25081 cctaatatgc cgaaattagg aaaagatatt gacgaagagt atgagcgttt atttggcgat
    25141 ttattagatg agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat
    25201 gaaagatttt tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta
    25261 caaaacagct attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa
    25321 ttatgcctac gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga
    25381 taagaatacc gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga
    25441 aacagttgat tggtatgaaa atatgggatg tattgttgtt agaactgact acaaagaagg
    25501 aaagtctagt aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact
    25561 gggcttggtt gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa
    25621 cgaatcgtgg ggcgaaagga gaatgattat gaaacacaaa tcattaatgc caatgaattt
    25681 gcaatacttt gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt
    25741 ggaatccaat gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa
    25801 acaattggaa gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga
    25861 atatgaggaa tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc
    25921 tgaatttgaa gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga
    25981 acaaattgct ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct
    26041 aaataatctt gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag
    26101 tgttttgaat gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc
    26161 tgcatttggt ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac
    26221 aaatccagaa gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt
    26281 atgaaaaaaa catctttaaa taatttagag tatttggata tttcaccagc gattaatgct
    26341 atgcaagtac caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca
    26401 gcaaactcga cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa
    26461 aaacttgagg gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat
    26521 actgaaattt ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat
    26581 ggagtcggaa atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat
    26641 ttgaaccgaa aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga
    26701 atgaatggga ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt
    26761 gcagtaacaa gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg
    26821 ggagaaaaac tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat
    26881 ggaaaatcaa caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga
    26941 aatatcgtgt ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga
    27001 acaattgccg caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca
    27061 gaagaacttg caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt
    27121 gaatacaatg cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt
    27181 agtactccct tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga
    27241 tgaagtcgtg aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa
    27301 aactcgtaga tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa
    27361 agaagcaatt gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg
    27421 atggatgaat cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg
    27481 agtgaagaac gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac
    27541 acttgtaaaa atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat
    27601 aaaataataa aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg
    27661 attagttatg cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg
    27721 atacgaagag ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt
    27781 tagaaactac ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta
    27841 atacaaatat tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc
    27901 tttcacaagg tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt
    27961 caactgacac atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa
    28021 taacaataac tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt
    28081 actatcctag tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa
    28141 tatgaatcaa atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa
    28201 aaagaaggaa tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca
    28261 ttaactcctg taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa
    28321 ctatcaagcg gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta
    28381 gaagaaggtc ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt
    28441 gcaacgaatc ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa
    28501 atcgttaaga aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt
    28561 aaaagaaatc tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga
    28621 tttttatttc gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca
    28681 aaatgtttct ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat
    28741 taatgaatta gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat
    28801 attatttcca aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac
    28861 attagaattt ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa
    28921 aaccggaatt atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa
    28981 tttcccggta tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc
    29041 taattatgaa acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt
    29101 tggtggtgca gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat
    29161 tactggaggg aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga
    29221 tggtgcgttt gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta
    29281 taaaggacag tttgctctag attcagatga aacgtcaacg attgaaggaa gtaaagtaag
    29341 ttttactcat ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta
    29401 ttcagttgat gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa
    29461 agtagcaatt ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat
    29521 caaacaacga ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt
    29581 aaatctgctg aatttttacc aggaacagtt gtagaagatg ctgcaggagt tatggaagaa
    29641 atgcaaacag cgactgataa acaatcagtt aaaaaagctt taagtcgtgc ttattcattt
    29701 attgcagata ctttatttga gggacaattt actggtgaag attattgcaa aggaattgat
    29761 gctcgtgaga ttgcctcatt gacaggaaaa ttattgaagt ctgttactgc aggttttgat
    29821 gaaacttata cagagacgaa aaaaaagtaa gtgaggcgct caagtcacct tcatttaagt
    29881 attcgattac ttaccgagaa ttagatataa agacgcaatt actcgaagca ggttggacgt
    29941 taccagaaat tgaacgtact gacttggatg agttaatgcg tctttttgct tttagagatg
    30001 cggtaaaaga gcatgaagat gttgagtact atgataattt cactcaattt taggaggtga
    30061 tactttgaat aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc
    30121 cggtatgaat aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag
    30181 tagaaatggt ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc
    30241 tagcggagta actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg
    30301 agcggttgat cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt
    30361 tagtaattca gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca
    30421 aggacttcct actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac
    30481 aggtgattta actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt
    30541 cggtggttca actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa
    30601 cggaaaagta gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct
    30661 ttctgctatt gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca
    30721 aggtaagatt tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg
    30781 cggaggactt aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc
    30841 tatacaaaat gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa
    30901 taaagcatta gtagattcag atttaggtgg atttaagggg attattgata aagttgccag
    30961 ttccatggaa tcttttttga aagtaattgc tgcaaatatt cctagagcag tgtctttttt
    31021 aagtaatctt tttgatgcag ttcaaaaatt tggctctgca ttgaaattca tgatgccatt
    31081 tctggttcct gcaactactg cttttggcgc attaatgttt caacttaaag gaataccaac
    31141 aattataaaa agcttcaata attttaagaa tgccataatc ggtgttggaa attcactaaa
    31201 gattatgggg gctatagccg ccgcaaatcc gtttgttttg attgttggag ccgttgttgg
    31261 agcgattgct gtatttggct attttatggc aaccaacgaa gaggttagaa acaaagttat
    31321 atccgtttgg aatgatgtaa aagattccgt acttggtgta ttaaagaata taaaagattg
    31381 gggaattgat acttggaatt ctgctaaaga aatggcatca aatgctgttg agagtgtcaa
    31441 ggatgcttgg tctggaataa aggaatggtt ttcaaatact tggcaaggca ttaaaaatgg
    31501 agcaacaggt ttatttgata aaacagtaga aacatctatg aatgcggttg atagtgtaaa
    31561 aaacgcatgg tcaaatacaa agcaatggtt ttctgatatt tggcagagca taaaagattc
    31621 agcaactgaa aaatggaatg aaattaaagg ttctatcatg gaagttgctg gtccattgat
    31681 tacaggttta aagaacgcat tcttgcatgt cactttttat ttagagacct tatggaataa
    31741 tctagttgag attggtaaaa atgtttttga gattttaaaa aatgttatac ttacgccagt
    31801 tttgtttatt acctccatga tttctggtgg atgggaagaa acaaaaaaca acatgattgg
    31861 tgtatggaat aatattaaag aaagtgctct aaatatttgg gagtctataa aaaatatttt
    31921 tgttagttac attacaaata tttattttgc tgcgcttaat atttggacag ggttcaaact
    31981 tactttgata aacatttgga atgaagtagt aagtcaagct aaatcaattt ggattaatgt
    32041 aaaatacttt tttattaatc tttggattga cattaagtat tttgctattc aaaaatggat
    32101 tgaattaaaa ttcgggatta ttcaaacttg gattgattta aaatataatg ctattactac
    32161 ttggaacaat attaaacagt tcttcaaaga tacttggcag aacattaaag atatagcata
    32221 caatacatgg atttctataa agaattccat gatcaatact tggaataaca tcaaggaatc
    32281 tttctggaat attgttactg gaattgttaa ctccgctgaa aatgcatgga ctaatctaaa
    32341 aaatggtgtt tcaaaagcaa ttaatcgggt gaaagaaatc tttgattcat taagggaaat
    32401 caatttattc gaaatcggta agaatatcat tgatggactt atcaatggtg tagtagaaaa
    32461 atggaatgca ttgaaaaaga ctattaaagg aatagctgga agtatcaaag attctattaa
    32521 gggtgcatta ggtattcatt ctccatctag atggatgagg gatatggtag gtaaaaatat
    32581 tgttcaaggt attatcgttg gtattgataa agaaaaaagt aaattggatc agacaatgac
    32641 tgatttagtg aaaactccgt cggttcaacc agttattaca gggtctaata gtcaaccagt
    32701 ggtacaagct aaacaaaata catcttcaaa tgcagtgaat gaaattcact tgcatttaaa
    32761 tgtttatgga gatctacctg attcgatgat taaacaaatc gccaagaaga tgaaaacaga
    32821 attgacgaga caaatgaaac gagatgctga tgcagtagga gggacattat atgcaactta
    32881 aaagaggaca attttttatc aatcaacatt attcttctga gtttaacgta tatattcaaa
    32941 ataggcctgc ctctgtttca gctagtcgtg taattgaatt gagagaacgt gaaggcaatg
    33001 actcaattat tatcgataaa gcctattaca aaaacgttac tagaaaaatt gaatgttatt
    33061 acaaagcacc atcgattgat ttagtgcagg aatgggaaga tcgaattact gaatggttag
    33121 atatgaactc ttacagtgat tttattcttt attacgatca acaatatatc tatcaagcta
    33181 tagtaaccga agcgccagaa tttaaaggaa caagaaaaac agggaacata gttccatttg
    33241 aatttacagt tagtcttcga ccattcaaag aaaattatag tggtcgtttt gctattcaac
    33301 agataaaagc tttcgagtta tttaatccag agaagtatgc ttcaaaaccg cttattaaat
    33361 tgagtggttc tggagatgct tctttttata ttaataatga taaatatgat ttgaaactat
    33421 tagatagaga attatatata gattctaaac tagaagagtc ttatcgaaag ctagatgaca
    33481 atttagaaca tcaagatcat gtcactttat ttttagattt tccatttcta taccctggaa
    33541 aaaatgaaat caaatggacg aataacatac attcgtttga gataatacca aggtggtgga
    33601 gaaaagtatg ataccaagaa tatatagtcc taccgagaca gatttttcta cgaatggttt
    33661 agggattttg aaggacacca caaaatgtga gatatatgaa gtagcaaatg gaaaatatga
    33721 attagagttg gagtatccat taggtactcg atttgatgaa tattttgaaa atgactatca
    33781 aataaaagca aagtcaaatg atcaagaaga gtatcatatc ttttttattg atgataaaga
    33841 tattgatacc tttttagata ccgtaactat ttatgcccag agtcgtacaa atcggcttgg
    33901 aagacgagcg gtcacttttg ctggagtcga ctctaaaact ggtcgagaag caatggcaat
    33961 tattgaaaac aatatggata aaaaatcaga tatacgctta tattctgata ttacaactgt
    34021 atcaagtaca acttttgaag caagaaatgt tttaaattgt attgctggtg aacaaggatc
    34081 tttacttcag tattggggcg gagaaattaa acgtgagcct tttaagctat ctttattaaa
    34141 gcgaagaggt cgtgataata ttggcacaat ccgatatgga aaagatttat ctgggttaaa
    34201 agttaagtta gattggacag gcgtgaaaac aagaattatt ccgtatgctg atcctcaaag
    34261 tgatgcaggt acaactagcc gaatttatgg ctcgccagta gatagcgaat atattaataa
    34321 ttatcctgat gtatatacag aacacgttca attcacagaa gaacagggag ttaaagacgt
    34381 taatagctta aataaaatag ctaagaatta tttcaaaaca attaatccag gctgtgataa
    34441 accgaagatt tctattaccg ttgagttcga taaattaacg gacaccgaag aaggaaaaga
    34501 attcgctaag ataagaaatt atgggttatt tgatacgttt aaaatatatc atcgaaaata
    34561 taaactatat tttgaatcga aagttagcgg tgttcaatat gattcattat ctgaaaaggt
    34621 tttgaaattg gaagctgggg atgctcaagt tgctttttat caacaacaag ctgttactat
    34681 tcaagataaa ttaaaagatt atgctacgaa taattatatg agtagtttta atgattatgt
    34741 gtcctctatg attgcaggtc aaggaaatgc aggcggttat gtagttttat ggccaaaaga
    34801 aaaaccttca aatattttta taatggatag ccctgattta aacaaggcaa aagaagtatt
    34861 aagaatgaac aaaaatggaa ttgccttttc taaaaatggt tggaatggtc cttttaattc
    34921 cgcttggaca ttggatagta tattcaatgc taattttatt caaacaggat taataaaggc
    34981 agatatcttt caaaactctt ttaataaaac tggagatgta ttgaaattag ttaatggact
    35041 acttcaaatt tggaataaca aaaagaaaat aatggagtta accaaaaaag gaatggagtt
    35101 ttggaattct aatagttcaa ttggaacgat tggaacaact gattctgctg gtaatccttt
    35161 tcctggagct tctactccca cacctattga agataattct ttagttattc gtacaaatgg
    35221 agacggcaaa tatattttga tttctcctaa agttggtaaa ggattagttt tattaggaaa
    35281 tggtaaagca atttattttg gagacttaga tgtacaaggc aaactcacag ttaacggaaa
    35341 agaaatcaca gggaacaaca gtggaggaag tgatcctgga actatccctc ctcaattgac
    35401 gacggaagct gaaaaaagag catggaaaat ttggacaatg ctgaaagctc gtggctattc
    35461 tgaatatgca gctgcaggta ttctaggaaa tattcagggg gaggttggag caagtatgaa
    35521 ccctgataca gaacaacttg gtggtccagc ttatgggatt gttcaatggg atggttctgc
    35581 ttatccgtta gtcggatcac caacttggaa cggacgagag tatgttcagc gtttgatgaa
    35641 caccgcaggg attcaagaag attatcgaag tattgaagcc caagtaaaat tattagattg
    35701 gtgtatgttc aacggtcaat ggctcggaaa agtaaatcca accacagtat caggatttaa
    35761 atcgatcaat gatgccaaaa gtgcagcgta tgcttttgaa atgaacttcg aacgcccagc
    35821 ttctgcacat ccagaacgcc aaaattatgc ccaatcttgg tataacaaat tacatggatt
    35881 aactagtcca gaacctggag ggaatttcat ttgtccaatt caaaaaccag tgacagttac
    35941 ttcagaatgt ggatggagaa ctagtccaat aaatggcggc caagaatttc ataatggaat
    36001 tgatcttgta aatggaaatc ctaatacacc tgtttttgca gcattagatg gggaagttgt
    36061 tcaggctggt gctaattatt atgactggta tggtaattac gtggttatta aacataataa
    36121 tgggaagtgg acaggttatg ctcatttgtc tcgtattgat gtttctgttg gacaaaaagt
    36181 ccagaaaggt gctcaaatag gcttgatggg aacaactggt ccatctacag gagaacatct
    36241 acattttcaa attatgaaaa attattggcc acagccagtt gttgattttg agaatccaag
    36301 aaattatatc caattttaag gtggtgattc tatgagtaaa tggaatgtcg ttttaagtac
    36361 aacagaacca tataattatg tggggatgat tcaagttcga caaggcaata agaatacaga
    36421 ggttatggaa gcgactatag ttgaaaatgg tcttccctac gatttatcag aatgtaaggt
    36481 atattttgaa tcagttgtag gtgggaaata tccagtccaa ttagaaacaa aaattgtgga
    36541 tgctaaaaaa gggaaaatta a
//
LOCUS       DACCCM010000018        35793 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCCM010000018 DACCCM010000000
VERSION     DACCCM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.019"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..463
                     /locus_tag="IVK78_000952"
     CDS             <1..463
                     /locus_tag="IVK78_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HAP6374439.1"
                     /translation="NGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKN
                     NSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDK
                     GISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            663..977
                     /locus_tag="IVK78_000953"
     CDS             663..977
                     /locus_tag="IVK78_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6374440.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            998..1375
                     /locus_tag="IVK78_000954"
     CDS             998..1375
                     /locus_tag="IVK78_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6374441.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1385..2158
                     /locus_tag="IVK78_000955"
     CDS             1385..2158
                     /locus_tag="IVK78_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374442.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2180..3583
                     /locus_tag="IVK78_000956"
     CDS             2180..3583
                     /locus_tag="IVK78_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6374443.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3765..4949
                     /locus_tag="IVK78_000957"
     CDS             3765..4949
                     /locus_tag="IVK78_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6374444.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4946..5236
                     /locus_tag="IVK78_000958"
     CDS             4946..5236
                     /locus_tag="IVK78_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374445.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5233..5454
                     /locus_tag="IVK78_000959"
     CDS             5233..5454
                     /locus_tag="IVK78_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374446.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5496..6275
                     /locus_tag="IVK78_000960"
     CDS             5496..6275
                     /locus_tag="IVK78_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP6374447.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6342..6836
                     /locus_tag="IVK78_000961"
     CDS             6342..6836
                     /locus_tag="IVK78_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6374448.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6892..7530
                     /locus_tag="IVK78_000962"
     CDS             6892..7530
                     /locus_tag="IVK78_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374449.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7602..7997
                     /locus_tag="IVK78_000963"
     CDS             7602..7997
                     /locus_tag="IVK78_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6374450.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7981..10434
                     /locus_tag="IVK78_000964"
     CDS             7981..10434
                     /locus_tag="IVK78_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6374451.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10431..12458
                     /locus_tag="IVK78_000965"
     CDS             10431..12458
                     /locus_tag="IVK78_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374452.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12455..13477
                     /locus_tag="IVK78_000966"
     CDS             12455..13477
                     /locus_tag="IVK78_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6374453.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13494..14423
                     /locus_tag="IVK78_000967"
     CDS             13494..14423
                     /locus_tag="IVK78_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6374454.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14668..14784
                     /locus_tag="IVK78_000968"
     CDS             14668..14784
                     /locus_tag="IVK78_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6374455.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14800..16719
                     /gene="tet(M)"
                     /locus_tag="IVK78_000969"
     CDS             14800..16719
                     /gene="tet(M)"
                     /locus_tag="IVK78_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6374456.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            16817..17005
                     /locus_tag="IVK78_000970"
     CDS             16817..17005
                     /locus_tag="IVK78_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6374457.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(17063..17416)
                     /locus_tag="IVK78_000971"
     CDS             complement(17063..17416)
                     /locus_tag="IVK78_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6374458.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            17947..18369
                     /locus_tag="IVK78_000972"
     CDS             17947..18369
                     /locus_tag="IVK78_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6374459.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18366..18596
                     /locus_tag="IVK78_000973"
     CDS             18366..18596
                     /locus_tag="IVK78_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6374460.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            19094..19294
                     /locus_tag="IVK78_000974"
     CDS             19094..19294
                     /locus_tag="IVK78_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6374461.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            19321..20514
                     /locus_tag="IVK78_000975"
     CDS             19321..20514
                     /locus_tag="IVK78_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6374462.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(20582..22147)
                     /gene="guaA"
                     /locus_tag="IVK78_000976"
     CDS             complement(20582..22147)
                     /gene="guaA"
                     /locus_tag="IVK78_000976"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6374463.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(22206..23129)
                     /locus_tag="IVK78_000977"
     CDS             complement(22206..23129)
                     /locus_tag="IVK78_000977"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6374464.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            23325..23930
                     /locus_tag="IVK78_000978"
     CDS             23325..23930
                     /locus_tag="IVK78_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6374465.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            24238..25254
                     /locus_tag="IVK78_000979"
     CDS             24238..25254
                     /locus_tag="IVK78_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6374466.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            25395..26696
                     /locus_tag="IVK78_000980"
     CDS             25395..26696
                     /locus_tag="IVK78_000980"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6374467.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            26742..27404
                     /gene="deoC"
                     /locus_tag="IVK78_000981"
     CDS             26742..27404
                     /gene="deoC"
                     /locus_tag="IVK78_000981"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6374468.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            27425..27823
                     /locus_tag="IVK78_000982"
     CDS             27425..27823
                     /locus_tag="IVK78_000982"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6374469.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            27889..28971
                     /locus_tag="IVK78_000983"
     CDS             27889..28971
                     /locus_tag="IVK78_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6374470.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            29183..30745
                     /locus_tag="IVK78_000984"
     CDS             29183..30745
                     /locus_tag="IVK78_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374471.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            30738..31871
                     /locus_tag="IVK78_000985"
     CDS             30738..31871
                     /locus_tag="IVK78_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374472.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            31871..32827
                     /locus_tag="IVK78_000986"
     CDS             31871..32827
                     /locus_tag="IVK78_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374473.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            32926..34092
                     /gene="deoB"
                     /locus_tag="IVK78_000987"
     CDS             32926..34092
                     /gene="deoB"
                     /locus_tag="IVK78_000987"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6374474.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            34116..34934
                     /locus_tag="IVK78_000988"
     CDS             34116..34934
                     /locus_tag="IVK78_000988"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6374475.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            34951..35658
                     /gene="deoD"
                     /locus_tag="IVK78_000989"
     CDS             34951..35658
                     /gene="deoD"
                     /locus_tag="IVK78_000989"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6374476.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11776 a         6218 c         7427 g        10372 t
ORIGIN      
        1 caacggtgtt gatttataca aatttgttac caaacatata tttgttaatc ttaatattga
       61 cgagaaaaat tacccgtcta tttttaagca ctatctattt tttattaacg aagtagaaga
      121 tcggataaaa aataattcat tatctagtag tgtatctagt tttaagaaga tgtttaaatc
      181 tcgagatggt atcacactta ctacagcaca taaagttaaa ggaaatgagt atgatactgt
      241 tatcgctatt aatcttctaa acgggaaaat tcctcattgg aatgaaattt ttaatgtttc
      301 tgataaagga attagttcag cgagaaaact actatacgta atttgttcaa gagcaaaaaa
      361 taatttattt cttttctcag aggtgggtta ttttacgagt aaaggagact tgctttatcc
      421 cactcaatta ttagataaaa tagaatttga ttataatatt taaaatacct aggtagtcgt
      481 tgttttaaat atagtaggaa tcggacgctc agtacggagc gtctattttt ttacccaatt
      541 ttgaaaggaa gtgaacttat gaaaataaaa aatcaagaat caaaaggtcg ctctcccccc
      601 tttaaaacca tcacacaaat aagattcatt caaacataaa aacagaaagg ataggtaaaa
      661 atatggaact taaatatgtc attcccaata tggaaaaaac atttggacaa ttagaatatg
      721 ctggtgaagg gaacattgaa caacgtcgaa taaatggcag accaaccatt ctctctcgta
      781 gttacaattt atactcaacg attcaacgag cagatgatat tgttgtcatt cttccctccg
      841 aagctggcga gaaacatttt gaatccgacg aaaaagtcaa actcatcaac ccacatatta
      901 ccgcagaagg ttataaaatt ggcaatcgag gatttaccaa ttacattatg cacgcagacg
      961 atatggtgaa agcataagaa aggaaaggtg aaaaaatatg agattagcag aaggtattgt
     1021 gattgaaaaa gaccagacat ttggcacatt gaaattttcc gccttgcgtc gtgaggttcg
     1081 tatccagaat gaagatggta cggtttccga agaaattaaa gaacgtacct atgacttaaa
     1141 atcaaaagga caaggacgca tgatacaagt cagtatcccc gcttctgttc ctttgaaaga
     1201 atttgactac aacgcagaag tggaattaat caatcctgtg gcagataccg tagcgacggc
     1261 tacttttcga ggggcagacg ttgattggta cgtgaaagca gacgatcttg tattaaaagg
     1321 acagacaaaa cctcatcaga aatataaaaa agaagcgtcc gaaagtgaaa aataggtgtt
     1381 gcttatgaca aacacaccat tgattttgaa agaaattccg aaggatgaag ccatttcttt
     1441 tattcggcaa taccattatt caaagatact ccctagattg tgtaaatact ttctagggat
     1501 tttttcagaa gaaaaattat taggcgttgt ggaattaggc tggggtacgc aacccctaca
     1561 aaccatacgc aaactctttc ccgacagttc ccttcaaacc acagattatt tagaaattgg
     1621 aaagatgtgt tttcttccag aaatgaatca aaccaattat tttggcagtc aagctctttc
     1681 agctctaatt aagtggttga aagaacatac ggactgtcat tttttatata cactggcaga
     1741 tggaattgag ggaaaatgtg gctatgtcta tcaagcgtcc aacttctttt actgcgggta
     1801 tttcaagaca agtgtctatc gtgataagca gtcatgggaa aagatacacc ctcgaagtgc
     1861 tcgcctttta ttagaggaaa atgctcgctt tgaacaggta gagaaaaagc attggctttc
     1921 ccaggcattt tgcgaataca aagggattga gaaaatcaat ggacgcatgt ttcgctatct
     1981 ctatccgttg acaaaagaag cgaaaaaact gttaggacac actctctatc gacgtcatta
     2041 ttatccgaaa gaaaaaaatt tacgctttga aaagcgaatc gcttatcaga aatatgaagc
     2101 catttctcaa ccaacttttg ataaacaggc tcgtatctac aatacacaat tattttgata
     2161 ggtggaaagg aggattttta tgccattgct tcatgacaga ggagagcgaa tcaaagccaa
     2221 cgataagcac ttggtctatc attttgtact aatttcattg ctggtagttt ttctagtcgt
     2281 cctactccct tttcattgga gagcgttaca atcggtgaac tggcaagcag ttcagttaga
     2341 tacatttttc tatcaacttc atacgccata cttttggagc agtattctca ctgctctttt
     2401 tgtatgcgga cttatcagct ggctttatta tcactatcgt atagattcta taaaacagtt
     2461 agagcaccgt caaaagttag ctcgtatgat tttagaaaat cattggtatg aagccgaaga
     2521 aactcaatca gagggattct ttaaagattt acccagcaag aaaaccaaac aacgaatccg
     2581 ccattttcct aaaatatatt atcgaatgaa agatggacta atccatattc atgtagaaat
     2641 caccttaggg aaatatcaag atcagctttt gcacctagaa aacaaactag aaacaggatt
     2701 gtattgtgaa ttagtggaaa aaacgttgaa agattcttat gtagagtatg tcctacttta
     2761 tgatactatt ggcaatcgaa ttggcattga agacgtggta gctcaagatg gacgtgtcaa
     2821 attgatggaa tcggtctatt gggagttcga ttctctccct cacatgttaa ttgctggggg
     2881 cactggtagt ggaaaatcct attttattct cacattgatt gaatctctcc tgcatacaaa
     2941 tgcaaagctt tatattcttg accctaaaaa tgctgatttg gcagacttag gggcggtcat
     3001 gaataatgtc tattaccgta aagaagatat gttggcatgt atcaatcaat tttacgaaga
     3061 tatgattgat cgcagtgaaa ccatgaaaca acaccctaac tataaaacag gagaaaatta
     3121 tgcttatctt ggtttgtctg ccaattttct tatttttgat gaatatgtgg caatgatgga
     3181 tatgctaggt cgtgaaagta ccaccgttat tcataaactc aaacaaattg taatgttagg
     3241 acgacaagca ggcttctttt taattcttgc ctgtcaacgt ccagacgcaa aatattttag
     3301 tgatgggatt cgggacaact ttaacttccg tgtggctttg ggtcgtatgt ctgaaatggg
     3361 ctttggtatg atgttcggaa gcgatacaca aaaacaattt ttcttaaaac ccatcaaggg
     3421 aagaggttac gtggatacag gaaaaagtgt gattagtgag ttttatacac cccttgtacc
     3481 aaaaaggtac gatttcttag gagaaatagg aaaagtcatt caaaaaaagc aatctgagcc
     3541 tgtaccgcac gaagtgaaag gtacaggttc agacttgctt tgattggtgt ggcgttagcc
     3601 acgccaatcc tttagccccc tctatctaac aggggggcac aaatacaact gaaaacaggc
     3661 aattctcatc aaccaatgtt gatggtacaa aggattgcgg aaaatatgaa aggtgtaaac
     3721 tcaagggtca tagtttacat cttttttaga ttggaggttt gcgaatggaa aacgaaaaaa
     3781 tttggattaa ggaattgaaa gaaaagcgac tggcttatgg cgtttctcaa aataaactgg
     3841 cggttgcttc tcatattacc agaccttacc tatcggatat tgaaacaggc aaggcagtcc
     3901 ctactcaaca ggtgaaagaa gatttactga acgctttgga acgcttcaat cccgataacc
     3961 cattggagat gttattcgac tatgtacgga ttcgattccc taccaatgat gtggctcaaa
     4021 ttatcggtga ggttttacgt ttgaatatgg attatatgct acacgaagac tttggctatt
     4081 attcttatcc cgaacactat cgtttcggtg atattgttgt cctagtctct catgatgtaa
     4141 gtaaaggtgt tttattagaa ctgaaaggaa aaggctgtcg gcaatttgaa aactttctat
     4201 tggctcaaca tcggagctgg tatgatttct ttcaagattg catggaacat aaaggtattt
     4261 tcaaacgctt ggatttagct atcaacgata aaacagggat tttgaatatt ccagatttgg
     4321 caaagaaatg taaacaggaa gaatgcattt ccgtctttcg aagctttaaa aattatcgca
     4381 gtggtgaatt ggttcaccgt gatgaaaaac catacatggg aaacacactt tatattggct
     4441 cattaaaaag tgaagtctat ttctgtatat atgagaaaga ttatgagcaa tacgtgaaaa
     4501 atgatgttcc cttagaagac gcagaagtaa agaatcggtt tgaaattcgg ttaaaaaatg
     4561 accgagccac ccaagccatg aaagatttat tggcacatca acaggcggaa aaaacagcct
     4621 ttgaaattat caatcgctat atccgctttg cagataaaga cgacactaaa cgtcggagtg
     4681 attggaaaac taatgaacgt tgggaatggt ttatcggtaa aaatcgtgga gcattacgcc
     4741 taaccacaca gccagagcct tattcttttg aacgaacatt gaactggtta catcatcaag
     4801 tcgcccctac attaaaaatc gcaagcattt tagatgttct caacggtaca accattatct
     4861 ccaccatgat tcaggaagcc aaactgacag aaaaacatga gaaattaatt gaacaacaac
     4921 atctagccat ggaggatttg attacatgag ccttttaaca aaagaattaa agaagttagg
     4981 gtttcagtgt ggcattgagt ttcaagcgta tatacaaaat acagggaaat atacgtctct
     5041 cattattgag ggaaaacggc aagctggtga taccatctat acctatgatt tttacaaggt
     5101 gagattttat aataattata ccaatcgagt aaccgtctat ggagagcatt taaccccttt
     5161 tcaattatta aggcgtgtaa aaagctatat ctattatcga gaaaaatatc ttaaggaaag
     5221 gaggacaatc acatgaattt tgggcagaat ttgtacaact ggttcttgga taatgcacag
     5281 tcattggtat tgttagcgat tgttgtcatt ggactgtttt taggttttaa acgagaattt
     5341 tcaaaactca ttggcttctt gattattgct ctaatcgccg tcggacttgt gttcaatgca
     5401 gctggcgtga aagatgtgtt attaaactta tttaaccgaa tcattggtgc ttaacagata
     5461 tttttcatga taaaatagga agtatatatt ttgttatgaa aggatttatg aatatgttta
     5521 caaatgatca atttaaatct cttttaatta aaacattaga aagaaagagc tcttcaaatt
     5581 attatgaagg aggtaacgag attgtttcta aaatgaaaat atcagaggta actttagatg
     5641 agactgacga ttttacttat tataaaggat ataaaaaggg ctggaatacg ctttgcacat
     5701 atcttaaaat aagagttcct tttgatgatt tagacttttt tgaaagtcat aaagatttga
     5761 ttacccagac agcaagctcc atatatgata aacaaggtga taatgtatta gtggatacta
     5821 ttttagtacc acttccagag aattacgaag taattaactt tagtcagttg aaaattagtg
     5881 atgtggtttc acaagctatt gaagacgctg agtcttttat gagtaatggt gaatatcaac
     5941 gtgcctttga cagagttcac actgcttttc atggatattt aattgaaata ctaaaaaaat
     6001 atgaaattac agttcctaga gacgaaaatc tatctaaatt atatagtagg atacaacaat
     6061 taattgaaaa agaaattcaa cctactgaac ttgctgatat agtgaagaca acgataaggt
     6121 catcaaatgg aatgattagc tctcttaatg aagcaagaaa taggcattca cttgcacacc
     6181 ctaatacaaa tattattgga aagagagaag caaaactaat aataggtatt tcaagtacag
     6241 ttacagatta tattagcgga taccttgata agtagcgtca ttaactgatt gctacttttt
     6301 ttatgactat ttttataaaa aggatgtgat aagaattgga aatgaaagtc tatgtggcaa
     6361 atttaggacg gtataatgaa ggtgaattag tcggagcatg gtttactcca ccaattgatg
     6421 aggaagaaat ggcagaaaga atcggtttaa atgaggacta cgaagaatat gcaatacatg
     6481 attttgagct tccttttgat gtggacgaat acacgccgat tagtgaaatc aaccgtctat
     6541 gtgaagccat tcaagaaatc gaaggtacac ctatttataa tgaactcaaa gaaattcaag
     6601 gcatgtggtt tagtagcttg gaagaattac tggaaaacaa agaggatatt cattgttata
     6661 gtgattgtga ttcgatggaa gatgtcgccc gttattacgt ggaagaaaca ggacagcttg
     6721 gtgaagtccc ttccaattta caaaattata ttgactacca atctttggga cgtgatatgg
     6781 aaatagaggg taattaccta gttacctctc atggggtatt tgaatattgt cagtaagttt
     6841 aacaatccgt gttataatta aattaataaa tagcacggag aggtataaat tatggtaaat
     6901 gtatttgaaa atcttaaact attaaaacta gacatgaaag ataaaggctg ggtgatagat
     6961 tccttttact ttcgttacaa acagcaaaat tatattgtac ttgttaagct atttgaaaaa
     7021 gaggaaaaag ttccagaata tgctctgtta aaactagaat ttttaaagga aaacgatttt
     7081 tcagatatgc tagctgtcta tgctaattcg gtaaaattat atacagacac gaaaacaata
     7141 agagaatatt ttggtatcga atacagtagt aacttgggag atgttttatt tcaatttagc
     7201 caaactcttg ctcgatttat tccgacagaa gttagtgaga agaaaaatga agaccaaaaa
     7261 gaagccatgt gtttctcatt aagtcaaagt gattcagaag atcccagaaa aaaatattgt
     7321 ttttcagtta gaagaaatcc tttaaaggga aatggtgagc tgggaaaacg aagtcctttt
     7381 aatgacaata aaacgagatt gtatagaccc agcttatatg aacgattagg gtcagatacc
     7441 aatttaagtt tcagatattc aatgaatcca gatgatgaag aaactgatga aggtattatt
     7501 gcaaaatgga caaaaaataa aattgaatag ttaaagcatt gaaagagcaa tcacctattt
     7561 tgattgttct ttttctattt ttacggaaag gacgtgaaga catgaaaaaa ataagaagtt
     7621 acaccagtat ttggtcagta gaaaaagtga tttatgcgat taatgattta caactccctt
     7681 tcccaattac ctttacgcaa atgacatggt ttatcgtttc tttgtttgtc gtcatactct
     7741 ttggcagttt accaccactt tctctgattg acggagcact actcaaatac ttagggattc
     7801 ccattgttct cactgggttc atgtgccaaa agacctttga cggaaagaaa ccctatggat
     7861 ttctaaagtc tatcattacc tatgctctac gccctaaagt aacctacgct ggcaaacaaa
     7921 tacgattaaa aaaagaaaca atgaatgaat cgattacgat tgtaaggagt gaacaactag
     7981 atggcatatc cgattaaata tatggaagat aatctcgtat ttaatcatga tggcgaagta
     8041 ttcgcttatt atgaattaat tccctataat tacagttttt taagttccga tgaaaaaatt
     8101 caagttcatg ataatttctg tcaattgatt gcccaaaatc gtgatggcaa gattcatgct
     8161 ctgcaactag caaccgaatc aagtattcga gccacacagg aacaatcgaa aaaagaaatc
     8221 actggacgat taaaagagat agcttacgaa aaaatagatg aacagaccga agcgttaatt
     8281 tctatgattg gtgacaatca agtagactat cgctttttta ttggttttaa actctacctc
     8341 aatgaacagg aagtatccgt aaaaaatgtt gggaaagaaa ttcaaaaagc atttgcggaa
     8401 ttctttcatg gtgtgaatca caaattaatg ggcgattttg tttctatgcc tatggcggaa
     8461 atcgaacgct tttctaagat ggaacagttg ttagaaagta aattatccag acgctttcag
     8521 attcgtaaat tggagaaaga tgattttggt tatattattg aacatcttta cgggcaaaca
     8581 ggtgtccctt ttgatgacta cgactaccat ttaccgaaac gatacgaaga taaaaaagtg
     8641 ttggtaaaga aatacgatat tctcaaaccc actcgctgtt taatcgaaga aaatcaacgc
     8701 tatttacgga ttgaacagga agataccact acttatgtgg cgtattttac aattaatagt
     8761 attgttggtg aattggattt ccctaatagt gaaatttttt attaccagca acagcagttt
     8821 actttccccg ttgataccag tatgaatgtt gaaattgtcg ccaataaaaa agccttatct
     8881 accgttcgca ataaaaagaa agaactgaaa gatttagata gccacgcatg ggaaagcgac
     8941 aacgaaacca gtactaatgt ggtggacgct ttagaatctg tcgatgaatt agaaagtaat
     9001 ttagaccaaa ccaaagaatc tatgtacaaa ctaagctatg tggtacgggt atcagcaaag
     9061 aatttggacg agctgaaaca acgttgtgat gaagtaaaag atttttatga tgatctgtct
     9121 atcaaattag tacgaccttt tggcgatatg ctaggcttgc ataatgaatt tattccctca
     9181 agcaagcgat atatcaatga ctatattcaa tatgtcacgt cggatttttt ggctggtctt
     9241 ggtttcggtg ctacacaaat gattggtgaa aattctggta tttacgtcgg ttacaatctc
     9301 gatacaggca gaaatattta tctccaacct agccttgcaa gtcaaggaat taaaggctct
     9361 gtaacaaatg cattagcgtc cgcttttatt ggttcattag gtggcggaaa atcattctct
     9421 aacaatatgc tggtctatta ttcagtactc tttggcggac aggcaaccat tgttgaccca
     9481 aaagctgaac gtggtgaatg gaaagaaacc ttacctgata ttgcccatga aatcaatatt
     9541 gttaatctca cgagtgagaa cgacaataaa gggttacttg acccttatgt gattttaaaa
     9601 ctcccgaaag attcagaaag ccttgccatt gatattctca cgttcttaac aggtatttcc
     9661 agccgagata gcgagaagtt tcctgtcctg cgtaaagcta ttcggagtgt aactcaaagc
     9721 aaacaacgag ggttgttatt ggtgattgat gaattgcgaa aagaagatac gcccatcagc
     9781 aatagtattg ctgaccatat cgaaagcttt gtcgattatg attttgccca tctgttattc
     9841 agtgatggca gtgtcaccca atccattagc ttggaaaaac aactcaatat tattcaagtg
     9901 gcagatttgg tcttacccga ttccaatagc tcttttgaag aatacactac catggaatta
     9961 ttaagtgtgg caatgctgat tgtcattagt acctttgctt tagactttat ccactctgac
    10021 cgttccattt ttaaaattgt tgaccttgat gaggcttgga gttttttaca agtggcacaa
    10081 ggaaaagctc tatctatgcg attggttcga gcaggacgtg ccatgaatgc aggggtctat
    10141 ttcgttactc aaaacgcgga cgatttacta gatgaaaaga tgaagaataa tattggcttg
    10201 aaatttgcct ttcgttccac tgacctagtg gaaatcaaaa agaccttaga attttttggt
    10261 gtggacaagg aagatgaaaa caatcagaaa cgcttacgtg aattagaaaa tggacaatgt
    10321 ttagtttcag acatctatgg tcgtgtaggg gttatgcagt ttcacccgat ttttgaagag
    10381 ttgcttcatg cctttgatac cagaccgcct gtgcgaaatg aggtggaaga atgacacaaa
    10441 aacaaaagaa atttcttcac tatagttgga taacgctcct ttgtgtgggc gttattttac
    10501 ttttattagg cacgctgggc aatgcagtac aagcgacagg cttagttgat gaaaccattg
    10561 atacaagcaa tgaatactcc aagtatggtt tgaatcaata tcagcttgat tattatgtgg
    10621 acaatagctg gggctggctt ccatggaatt ggagtgatgg cataggacaa tcggtgatgt
    10681 atggattgta tgccattact aattttattt ggacaatttc tctttatcta tcgaacgcca
    10741 caggttattt ggtgaaagaa gcctatgcct tagatttcat tagtcaaaca tcccaagcaa
    10801 ttggcgaaaa catgcagacg attgcaggca ttacaccgaa tggcttttcc agttcagggt
    10861 tttatgtcgg tttcctcctt ctttttattc tcattgttgg aatctatgtt gcctatacag
    10921 gattggttaa gcgagaaacg acgaaagtga tacgagctgt tttaaacttt gtcgtggtgt
    10981 ttattctatc ggcttccttt attgcgtatg cacccgatta tattgcgaaa atcaatgatt
    11041 tttccaaaga tgtcagtacc gccagtctga atattgggac aaaaattgtt cttcctaaca
    11101 cggatagtca agggaaagac agtgtggaca tgatacgtga cagcttattc tctgttcaag
    11161 taaagcaacc atggctattg cttcaatatg gctccactga tgtagaagcg ttaggggaag
    11221 aacgtgtgga aagtttattg tctaccagtc cagatacaaa caatggtgaa gatagagaaa
    11281 atgtagtgat tgaagaaatt gaagatcatg acaataccta tttaacccta ccgaagacga
    11341 tttccagatt aggaacagtc ttctttttgt ttgtattcaa tattggtatc tcgatttttg
    11401 ttttccttct aacaggaatc atgatttttt cgcaagtgct atttattatt ttcgctatgt
    11461 ttttgccaat cagtttccta ttaagtatgt tgccgagttt tgatggcatg ggcaggcatg
    11521 ccattaccaa gttatttaat gtgattatga caagggctgg tattaccttg attattaccg
    11581 ttgcctttag tatttccact atgctttatt cgctgtcagc aagttctccc tttttcatga
    11641 ttatgttttt acaaattgtg acctttgcag ggatttattt taaactgggc gatttaatga
    11701 gcctattcac tttacaaagc aacgactctc aaaacatggg cagacaagtg tttcgcagac
    11761 ctcgtcaaat gatgaatcga caaaccagaa gatttcaacg cagtatgagt aagatttttc
    11821 gtggaaaagc agggtcaacg aagcaaaaga caatgacaac aaagcagaat caaaccaata
    11881 aagctaacca tactcgtcaa aatgaacgga ctacaccaaa gaaagaaaca gcactttcca
    11941 atgtgaaaaa gcaacaaggc ggtatcgggc aacgattagg tgaaaagaca gcccatgtat
    12001 tggacacgaa aaatcgtcta gtcaataaaa gaaagcaggc aaaacaacag atacaggcga
    12061 caccaaccca tgtcaaatat gcccttcatc aaggaaaaga gaatgtcact cataatgtaa
    12121 cagactttaa acaatctatg gcagatacca aagctcaacg caaacaacag cgtatgaacc
    12181 agcaggagca aaaacgaaag aacattgctc aaaagcgttt ggagctggat aaagcaaaag
    12241 aaaaaagacg aaaagaaaac ccatcatctc ctgttcagaa taaccagcag catgttcgca
    12301 agcgtcagca aaccgtcacg cctgttttgt cacaaaacac taaacgtgaa aatcatcagc
    12361 gaccattgaa aaaagagcaa aaacaaaaga caccaccgaa gcaattcaat cagaggaagc
    12421 taaataaaag aacggataag cgaggacaac aaacatgaag ctaaaaataa cagtgatttg
    12481 tagtgtggtc ttctctctct tcttttttct cttattattc ctagtcattt tctttgcaga
    12541 tgatacggac agtggcgaaa acaacaagga ttcttctatt ccacaaggcg gtgtgaccgt
    12601 ttcaccagaa gtgttggcac accgtccgtt gattgaaaaa tatggtaagg aatatgggat
    12661 tgaagactat gtttcttata tcttagccat tatgcaagtg gaatcgggcg gtacagcaga
    12721 agatgttatg caaagttccg aatctttggg tttaccgccc aacagtttga gtaccgaaga
    12781 gtccatcaaa caaggggtta aatatttcag tgaattatta accagtgcag aacaacaagg
    12841 agtggatata gattctgtta tccaatctta taattatggc ggtggctttt taaattatgt
    12901 gaggagtcat gggaaaaaat atacctatga attagccgaa cagttttcta aagagaagtc
    12961 gggtggtcaa aaagcagact atcccaatcc tatagccata cctgtgaatg gtggttggcg
    13021 atataactat ggaaatcagt tttatgttca gttggtttca caatacctaa ccgacacttc
    13081 cccaacagaa tttgatggtg aaaccgtgca agtcattatg gacgaagcct tgaaatacga
    13141 agggtttccc tatgtctttg gaggggcgtc gcctaccacc tcttttgatt gtagtggctt
    13201 aatacaatgg gtgtatgaca aagctggcat ttctctccct cgtgtcgccc aagatcagta
    13261 tgacgctacg caagaaatct ctatggaaga agcccaagca ggcgacctta tcttctttca
    13321 ttcaacctat aatgcaggca catatgtgac tcatgtggcg atatatttag agggaaatcg
    13381 cttttatcat gcaggcgacc caatcggtta tggcgactta agcagtcgtt attggcaaga
    13441 ccatctgatt ggggctagac gtgttattca taactaagaa aggaatgaat caaatgatta
    13501 taaaaatcga gcgaaaagaa aagaaggaga aaccaaagaa atctaaaaaa ccgagcaagc
    13561 aaagaaaagt tccgatgatt aaagttggca cgcataagaa attaaccttt gttttatggg
    13621 ttttattgat tggtagtgtc ggatttggaa tctataaaaa ttttactgcc attgacactc
    13681 atactgtccg tgaaacagaa atcattaaac agcagattgt cgataccaac caagtagaaa
    13741 gttttgtgaa atcctttgcg aaagactact tttcgtggca acaatcccaa gaagccattg
    13801 ataaacgaaa tgaaaaattg acccattatt tgacggaaga attgcaggta ttaaatgaag
    13861 aaatgattcg taaggatatt cctactagtt ctagtgtgaa cgatatacag gtgtggcagg
    13921 tttctcaagt gaatgagaac acctttgagg tactgttttc tgttgaacaa gttattaccg
    13981 aagacaaaga caaggaaact atatcgtcta gctttcatgt ggttgtccat atagatgaat
    14041 cagataatat ggttattatc aaaaatccga caatgagtaa gaaacctcaa aaatcggact
    14101 accaacccaa acagctagaa agtgaccata cagtagatac agaaacgatg gacgaaatca
    14161 ctagcttttt agaaacgttc ttccagctct atcctaccgc cacagaaaaa gaattgactt
    14221 actatgtaag taatcatgtg ttacctatga ttaacaagga atatgttttc gaggaattgg
    14281 tgaatcctat ctttaccaga aaagataacc aagtaatcgt gaatgtcgct gtgaaatatt
    14341 tagatcaaga aacaaaggca acccaaatct cgcaatttga gttgatatta gaaaaacaag
    14401 ataattggaa gattgtaaaa taacaaatat tggtacatga ttacagatac tttgtaatca
    14461 tgtactcttt ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta
    14521 tttaagtgac tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg
    14581 tcaatttgat agcgggaaca aataattaga tgtccttttt taggagggct tagttttttg
    14641 tacccagttt aagaatacct ttatcatgtg attctaaagt atccagagaa tatctgtatg
    14701 ctttgtatac ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat
    14761 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
    14821 gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt
    14881 ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt
    14941 ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat
    15001 acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt
    15061 tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
    15121 caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc
    15181 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
    15241 ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg
    15301 aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag
    15361 aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga
    15421 tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt
    15481 gataacctta tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct
    15541 gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat
    15601 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
    15661 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
    15721 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
    15781 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
    15841 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
    15901 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
    15961 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
    16021 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
    16081 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
    16141 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
    16201 tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa
    16261 ggattatatg gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat
    16321 agccctgtta gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc
    16381 tttagaaaag ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca
    16441 caggaatatc tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat
    16501 actcaactga aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa
    16561 gattatcgca atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta
    16621 aaaggatatc aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg
    16681 atagataaag taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata
    16741 aatatggttt cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg
    16801 aaaggaggag attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa
    16861 aacaagacta cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa
    16921 atgtaagaac gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc
    16981 agacgccaag acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt
    17041 ttgtttatct gaattttact gactagcctt caatatttct tgcttcattg ataccttttg
    17101 ctagtgcttc cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct
    17161 gtaaccttcg agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata
    17221 catggagtaa tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt
    17281 ctatatttgt taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga
    17341 gaccccgttt cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat
    17401 cttctttttt acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa
    17461 acaggtatag aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta
    17521 ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact
    17581 ttataaaatt tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa
    17641 agtcgccatg agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa
    17701 acttatccac ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat
    17761 agaaaaatca aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt
    17821 aataaatatc ttttaggatt tctcttcatt tatgcaacaa atcccccttt cgtgtcgggg
    17881 tttagtatga agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg
    17941 aaaagcatga aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc
    18001 aaacaagtta ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca
    18061 aaagaagtct cgttttctga tatagataac taccttgtca actctttttc tactgttgat
    18121 acgtacgtca ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt
    18181 gatttattaa gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt
    18241 cattacttta tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg
    18301 actgtttatc gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt
    18361 aaagaatgaa aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg
    18421 gcgacgtgga agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt
    18481 cattacgtcg catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac
    18541 gtggcagaat ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc
    18601 attttttcgt ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat
    18661 tccatcaaag catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt
    18721 tgacaaccaa ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca
    18781 cgccatgacc tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc
    18841 aggattgctg ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga
    18901 gcgttagaag cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt
    18961 cctgtgagct ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta
    19021 ttataaaact atttgatata attataggtg aatattggag ttttcctagc ctataattgg
    19081 gaaaggagta ataatgataa cggtcacaaa aaaggattta gttgagctag gatatggaac
    19141 ttctttttca gcagacatca taaaaaaagc aaaagaattg atgattacaa aagggcatat
    19201 ctactatcaa tctcgaaagc tagatagagt ccctcgtgaa gctgttgaag aaattctagg
    19261 aattcggttt tcaaatgaaa acaggtctga gtagtttttg cacagtaagg agtaaaaact
    19321 atggttaaag acccaataaa aaaagcaaag aatggaacct attattttcg tgcaaatcta
    19381 ggttatgatt ctaaagggaa aaaaattcaa aaatatcgta gtggattcac tactaaaaaa
    19441 gaagcaagag aagaatattc gaaactgcta ttgatgaaac cagaagaatt aagtgagaat
    19501 aaggataaaa tgacgtttaa acattatatc tttgaaattt tccttccatg gtataagaca
    19561 caggtaaagc tcagaacata tgaaaatcgc ttaccaacta ttaagaagca tttctcttat
    19621 tttgataaaa tggctgtttc cgatattgag cctattgatg tgcaaaattg gcaactaaaa
    19681 ttatcaaaga agtgtaagtc ttcttatgta agagctgttc aaggactatt ttcagttgct
    19741 atggatcgag caattgtcct tgggataacc accacaaatc catctaagat tattggcaat
    19801 gtgaaaaagc aaaagtctaa aattgaattt tggacaaaag aagaatttga aaaagtaatc
    19861 tctctaattt acaaagaaga ttactatcaa cactttttat ttatctctct gtggttttta
    19921 tttatgactg gaatgcgaat tggtgaagct actgccattc aatgggaaga tattgatttt
    19981 gactcaggag tactaaccat caacaaaaca ctctattaca agaatcaatc gaattataga
    20041 tttgttgaac caaaaacaaa ggcgagcgtt cgtcatattg tattagataa atgcacatta
    20101 acttatttat ccgaatggaa ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg
    20161 agctataacg gtattcctac ccaaaaacat acactatcat atgctattga acgctatgct
    20221 aagctggctg gtatccatcg aatcagaatc catgggttac ggcattctca tgcttccttg
    20281 ttaatcagta tgggagaaaa tccacttatc attaaagatc gtctaggaca cgaagatatt
    20341 gagacgactt taggaacgta tggacatctt tatcctaaca gtaatttcga ggtagctaac
    20401 aaattaaatg gagccatttc ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag
    20461 aatcaattta ctgtagacta cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc
    20521 agtaaaaaga taagaagagc cagaaaccct tggtataaga ggatttctag ctctcctaca
    20581 actattccca ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga
    20641 cgtgggctac ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac
    20701 gggcaaagtc agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt
    20761 cgtaagtacg tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat
    20821 attgccagat atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag
    20881 attcgcgtac gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac
    20941 caggaccagg gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta
    21001 attcacggac ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat
    21061 cttctggcag tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag
    21121 attcgataac gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag
    21181 caagttttgt tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt
    21241 tttgttctgg gtcagataca cctttcaact tgtctaagaa gcgatctctt gcatcgactt
    21301 tgatgatgtt caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt
    21361 tgcgtagcag accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta
    21421 aaagaacccc aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt
    21481 caccaacttg ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt
    21541 taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg
    21601 aatgacgtac ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag
    21661 aagcaatcgg acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta
    21721 ccaagtcacc atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag
    21781 cttgatcgct tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta
    21841 cggaaccgcc aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa
    21901 tacctaaatt gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg
    21961 gtccgccaga aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc
    22021 ggtggctcaa taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt
    22081 tatactgact accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt
    22141 ttgtcaaatt tattacccct atccctttaa attttatctc aaaatagtca aactatttta
    22201 aaatgctaat attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga
    22261 attatatcgg ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca
    22321 tttttccata catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga
    22381 taataataat tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa
    22441 taccattttt cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg
    22501 ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag
    22561 atatcaggct gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag
    22621 taaacaggta cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc
    22681 atctccatat cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat
    22741 atacgatttg gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag
    22801 gtacgagaca aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt
    22861 ccggcgatcc cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta
    22921 ctcaagaaca aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca
    22981 tagatatctc gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt
    23041 tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc
    23101 acacggtagt agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa
    23161 ctattttaac acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg
    23221 tataatcttt ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa
    23281 aaggatagac tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact
    23341 attctgaacg cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca
    23401 gaacctttca atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt
    23461 cacgtgtcct gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag
    23521 atgtgggatg cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg
    23581 ttgaaatggt cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta
    23641 accaaatcga acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa
    23701 cctatgcagc tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa
    23761 ttttgacagg cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga
    23821 agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa
    23881 tcgtcacaaa agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa
    23941 aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt
    24001 tttttgttga gaagttttta aaaacaaaga actatttttt cttcaaaaag aaatagtagg
    24061 aacagagtcg ttgtttttta cgataaaata aataagatac agaacttgta tagaaggctg
    24121 ccaagtcggt agataaagga ggtttttatc aaaaaacata ctttcctttt ttctctattc
    24181 ttttcttggg ggttttggta cactgatagg gaatttagat agaagggagc caataggatg
    24241 aagctgacca tcagagatat cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag
    24301 attttgaata acaaaggaag cagatttagt gataagacaa gaaaaaaagt gttgtcgatc
    24361 gtgaatgaac atcattataa gcccgattat ttcgcttcaa atgtgatcaa tcgacattca
    24421 aagaccatcg gaatgatcgt ccctgatgtc actgatttct tcttctccaa actgatcgaa
    24481 ggtgtagaga gctatttgaa tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat
    24541 agccaagaaa atgagatcaa gtatttacaa gagctttccc accgctcagt ggacggaatc
    24601 ttgctggcca caccaaatat tttacctgag aaatatgcat tagacagcgg tttttatcaa
    24661 aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc gggataacgg acggcttatc
    24721 gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa tggccatacg
    24781 aagatcggca tgctgaagga aaccactgga tactatcaat tagaagaacg tttcaacgga
    24841 tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt tgaacatgga
    24901 gagttgactg tccaaggtgg atatactgca tcaaaaaaat tactcaaaca taaagatatc
    24961 acggctattt tttgcgggaa tgatgccatg gcgatcggat gctatcaggc gatcgatgaa
    25021 ttgggcaaaa aaatcccaga agatatctca gtcataggat tcgatggatt aaagctgtca
    25081 gaatacatga ttccgcaatt gacgacagtg cagcagccaa gttttgatat tggattttat
    25141 gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg
    25201 ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc
    25261 ggtatattgc ttatgtaagc atttacaaag gctagttagt gcttttttta tttttagtaa
    25321 accagtttac taaagtggtt tacctttcct aaaaataatg tataataccg aatgaagaca
    25381 aggaggatca ggcaatgcga atggtggatc tgattgaaaa aaagagggat ggaaacgagc
    25441 tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac gaatggaaag attccagatt
    25501 atcaagtcag tgcattattg atggcaattt tttatcaaga catgacaaat gaagaaatca
    25561 cgaacttaac attggcgatt gctaattctg gtgatgtcat cgatttgagt tctcttgaag
    25621 gaataaaggt agataagcat tcgacaggtg gcgttggtga cacgaccaca ttgattttag
    25681 cccccctagt agctagtgtc ggagtgacag tggctaaaat gtctggacgc ggtctaggtt
    25741 acacaggtgg tacactagac aagctagaag caattcccgg atttcagatc gagctttcgg
    25801 atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa
    25861 acttggcacc ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg accgttgatt
    25921 ctcttccgct gattgccagt tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa
    25981 tcgttttgga tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac
    26041 gtctggcaaa gaccatgacg agcattggga aactggctaa ccgagaaaca gtggcggtta
    26101 tctcagatat gtcagaacct cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg
    26161 caatcgaaac attacaagga aatggtccag aagatctagt agaaatgtgc tacgccttag
    26221 gaagccaaat ggttgttctt gctggcaaag ctaagacaac cgatgaagca agaacgttgc
    26281 ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc
    26341 aagggggaga cccagcgatc gtggaacatc cagaacgaat tttgactgca cgctacacaa
    26401 tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa
    26461 ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga agaagatatt gatcacgctg
    26521 ttggattgaa actgcataaa aaaattggtg acactgtgac aaaaggtgag tcactattga
    26581 cgatctatag taacgacgaa gaaatcactt ctgtcattga attattatat aaaaatattg
    26641 aaattggtga atcggcaatg aagccaacgt tgattcatga catcatcaca gaataaagga
    26701 aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga
    26761 tcgaccatac tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag
    26821 aagcaaaaaa ataccatttt tattctgtct gcatcaatcc agcatgggtt gcgctagcga
    26881 aagaacaatt acaaggtgag ccagtagccg tatgtacagt aatcgggttt cctctaggag
    26941 caaacacacc cgagacaaaa gcatttgaaa caacagatgc catcaataac ggtgcagatg
    27001 aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa
    27061 aagatatcga agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg
    27121 aaacatcgtt attagataga gaagaaatca tccgagcttg cgaaatagca aaagctgcag
    27181 gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag gtagaggatg
    27241 tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc
    27301 ataacgaaga agaagcaatg gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg
    27361 caggagtagc aatcgtatct ggttcaactg gtgaaggcta ttaatttttt caaaggagaa
    27421 gctcatggca gaactcaaac aagaatggat caacacagct attgaagcgt tagataaagc
    27481 ctatgttcct tattcccatt ttccggtagg cgcttgtctc gtaacggaat caggcaagat
    27541 ttatcaaggc atcaacattg agaatgcatc atttggatta acgaattgtg ccgagcgcac
    27601 agcttttttc aaagcagtat ctgaaggaga aaaaacattt acgcatctag tcgtagcggg
    27661 gcacaccccc gatcctatct ctccttgtgg tgcatgtcga caagtaatgg cagaattctg
    27721 ttcccctgat atgccggtta cgctagtagg agacaatggt gtgacgaagg caacgaccgt
    27781 tcgtgcactg ctgccttatg catttactga aaaagatcta taactcatgt tcattcagtc
    27841 agctgactgt tgaaaaataa aacaattatt ttaggaggct ttacagaaat gaaaaaagca
    27901 aaattatttg gtttaggtgc tgtcgcacta gcagcagggt tatttttagg tgcatgcggc
    27961 aacaacggct ctactgattc atccggtggc aaaagctcat cagacacaac aactgcagca
    28021 ttgatcacag acacaggtgg tgtagacgac cgttcattca accagtcagc ttgggaagga
    28081 ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa atgatggctt ccaatatttc
    28141 caatcttcaa atgaatcaga ttacatccca aatattgacc aagcattgaa tgctggattc
    28201 aaaacgattt ttggtattgg ttataaacta aaaccagcga tccaagaaca agcaacaaac
    28261 aatacaggta cgaactttgt tattatcgat gatgtcatcg atggattaga taacgttgta
    28321 tctgcaactt tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc agcttatacg
    28381 actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt
    28441 gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa
    28501 gtattgaatc aatatgctgg tgacttctct gctccagata aaggacgttc aattgctcaa
    28561 ggtatgtatg cacaaaacgc agatatcatc ttccatgctt caggcggtac aggtaacggt
    28621 gtattccaag aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt
    28681 gttgaccgcg accaatcaga cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc
    28741 acattgacat ctacactgaa agcagttgga acagtagtag aagatttggc acaaaaatca
    28801 gcggacggta aattccctgg cggcgaacac actgtttatg gattgaaaga agacggtgtt
    28861 ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa
    28921 aaaatcattt caggtgacgt aaaagttcca gaaactcctg aagaaaacta aaaccatttc
    28981 tatagccttg ttagacacat gtttgacaag gctatggttt gattagaaca agaattgttc
    29041 atttatccaa gctgccttgt gacagtttgg ataagggaac aactccctga agtaatgttt
    29101 gtttcaaaag aagaacgctc actaagcgtg aatcttagtc aatcgttgcc ctagatagaa
    29161 atcgttagga tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa tgcagaatat
    29221 taccaaagaa tttggaacat tcaaggcaaa tgacaacatc aatttacaag taaaggcagg
    29281 agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa tctacattga tgaacgtgct
    29341 ttccggatta ttagagccga catcagggaa aattttgatg cgtgggaaag aagtaaagat
    29401 cacaagtccg accaaagcca accaattagg gattgggatg gtccatcagc actttatgct
    29461 tgttgatgcc tttactgtaa cagaaaacat cgtgttggga agcgagccta gtcgcgcagg
    29521 gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac agtatggatt
    29581 gtcagtcaac ccagatgctt acgttcgtga tatttcagtt ggaatggaac aacgggtaga
    29641 aattttaaaa acactttacc gaggagcaga tgtactgatt tttgatgagc cgacagctgt
    29701 attgacccct caagaaattg atgaattaat cgtgatcatg aaggaattag tcaaagaagg
    29761 caagtcgatc attttgatta cgcataagtt agatgaaatc aaagcagtag ctgaccgctg
    29821 tacagttatc cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg taacctcaca
    29881 acaattagcc gatatgatgg ttggaagaac ggtttcattc aaaacgatga aaaaagaagc
    29941 gaagcctcaa gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa atcgtggatt
    30001 agaagccgtg aaaaacttga acttagaggt tcgtgctggc gaagtacttg gtatcgctgg
    30061 aatcgatgga aacgggcagt cggagttgat ccaagctttg actggtttgc gaaaggcaga
    30121 aagcggacat atcaagctaa aaggggaaga catcactaat aaaaaacccc gaaaaatcac
    30181 tgaacatggt gtaggacacg tgccagaaga ccgtcataaa tacgggttag tcctagatat
    30241 gacattatct gaaaatatcg cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg
    30301 catgctgaat tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt
    30361 tcgaacaacg aatgaacttg ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc
    30421 aattatcgct cgtgaagtcg accgagatcc tgatctgttg attgttgcaa atccaactcg
    30481 tggactggat gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac aaagggacaa
    30541 atacaaagca gtgttactga tcagtttcga attagaagaa attttaaatg tttcggatcg
    30601 tattgctgtt atccatgaag gagaaattgt cggtatcgtt gatccgaaag aaacatctga
    30661 aaatgaatta ggtctattga tggcgggcta taccttagaa gaagcacgaa aagagttaga
    30721 aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg
    30781 tactttcagt tgttttagga ttgatcttag gcgcgatatt gatggctgct tttggttttc
    30841 agcccatcca aggttattct tctatgctag atgcagccct tggttcgcaa agagggatag
    30901 gtgaaacgtt aaggcaagca acgccgttga tttttacagc attaggtttt tctgtagcta
    30961 actcagctgg gtttttcaat attgggcttt ctggacaagc actttgcggg tggatcgtca
    31021 gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt tgttttgctt ccgttatgcg
    31081 tgatcttagg tgccttagct ggtgcgatgg cagcggctgt tccaggttta cttcgagcat
    31141 tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa
    31201 gcacatttgc tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg gactcttcca
    31261 atatgatcag tcaaaatgcc tcactgcggt taccatggtt aacacaagct tttggcggct
    31321 ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct tattttcgtt tggctgctga
    31381 tgaagaaaac gactttagga tttgaaatcc gttctgttgg attgaatcct tttgcttctg
    31441 agtatgcagg aatgagtagt aaacgtacga tcgttttgtc gatggtcatt tctggcgctt
    31501 tggcagggct tggcggtgtg gttgaaggat taggaacgta tcaaaacttc tttgttcaaa
    31561 cgacttcgct atctattgga tttgacggaa tggctgtttc cttgcttggt tctggatcag
    31621 caatcggtat tcttttatca gcgatcttgt tcagtatttt gaagatcggt ggacttggca
    31681 tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct ttgattatct
    31741 tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa
    31801 aacaagaaga gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac catgaagggg
    31861 gcgaactata atgtcgacta tcgaattaat tgccttgatc cttacttcta cactggttta
    31921 ttcaacaccg ctaattctaa cgtcactagg cggaactttt tctgaacgaa gcgggatcgt
    31981 aaacgtaggg ctagagggaa tcatggtcat gggtgctttt agttccatcg ttttcaactt
    32041 gacgatgagt ccatcacttg gttctatgac accatggatc gctattttag tgggtggcgt
    32101 cgtaggtatc ctattttctc tgattcacgc agtagcaaca gtcagtttgc gtgctgacca
    32161 tattatcagc gggacagtaa ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa
    32221 agcatggtat ggtaaaggtc aaactgataa catcacgcag aatctggggt atttttcttt
    32281 cccaggatta gcagatattc cggtcattgg accaattttc tttaaaaata cattcttacc
    32341 tgcttattgt gctattttga ttgctgtcct agcttgggtg atcatcttca agacgaaatt
    32401 cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct gccgacacat taggtatcaa
    32461 tgtatatgct atgcgttatt caggcgtact gatttctggc ttgttaggcg gtatgggcgg
    32521 tgctgttttt gctcagtcga tttccggtaa cttttcagct ggtacgatcg ttggacaagg
    32581 gttcatctcc atggctgcta tgatctttgg taagtggaat cctttagggg caatgggtgc
    32641 ctcattgttc tttggttttg ctcaaagctt gagtatcatt ggcgcacaat taccagttat
    32701 ttcttcgatt ccgccagtat tgctgcaaat tgctccatat ttgctgacaa tcattgtgct
    32761 ggttgttttc ttagggaaag cttctggtcc aaaagcaaac gggaagaact atatcaaatc
    32821 aaaataatct tttagcgaat aaaaagggtg ggttacggga agtgtttttc gtctcgccct
    32881 ttttgtttta aaggcaataa aaataaaaag aaagaaggaa taatcatgtt taaacgcgta
    32941 catctgatcg tcatggattc agtaggaatt ggagaagcac cagatgccga taagttcggt
    33001 gatgttggtt ctgatacatt aggacacatt gcgaaagaag caggattgaa cgttccacat
    33061 ttagagcagc ttggtttagg aacgatccgt cctcttgaag gtgtggaatc agtgaaagat
    33121 catcaaggct atgcgacgaa attagaagaa gtttctgtag ggaaagatac gatgacaggc
    33181 cactgggaaa tcatgggatt gaatatcaaa actccatttc gtgtgtttcc taatggattc
    33241 cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa
    33301 ccttatagcg gaacagctgt tattgacgat tacggtgaac accaaatgaa aacaggcgat
    33361 ttaattgtat atacttctgc cgatcctgtc cttcagatcg cggcacatga agaaatcatt
    33421 cctttagaag aattatatcg gatttgccaa ttcgttcgtg atataacaaa agatgatccg
    33481 tatatgattg gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga cttcacacga
    33541 acaagcaatc gccatgatta tgcgttgaat ccattcggac acacagtcct tgattctttg
    33601 aaagaagcag gaaaggacgt gattgctgtt ggaaagatca acgatatttt caacggacaa
    33661 ggaatcactg aatctgtccg cacgaaaagc aatatggatg gcgttgacca gctgctgaaa
    33721 gttatgaaaa acgatttttc aggaatcagc tttaccaatt tagtagattt tgatgcatta
    33781 tacggtcatc gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt tgacttacga
    33841 attccagaat tgcttgaagc gatggaagaa gacgacttgc taatgattac agccgatcac
    33901 ggaaacgatc cgacgtttac tggtaccgat catacgcgag aatatgtacc gttattggtt
    33961 tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag gatattattc agatatttca
    34021 gcaacgattg ctgagaactt tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa
    34081 ttattaaaat aatcttttta aataggagaa agcaaatgac caagttaagc gagatgttac
    34141 aagaaacgac ccagtttatc cttgataaag gagtaaaaga aattgatttt ggtttgattc
    34201 taggttcagg attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg atttcttatg
    34261 atgagatccc gttctttcct gtttcgacag tagttgggca tgcaggaaag ctagtttatg
    34321 ggtctctggc aggcaagaaa gtgttagcca tgcaagggag attccactac tatgaaggtc
    34381 attccatgca aacagtgacc tatccagtac gtgtgatggc cggactaggg gcccattcag
    34441 tcattgtgac taatgcatgt ggtggtgtca acgaatcatt tgtaccagga gatttgatgc
    34501 tgattaccga tcatatcaat tttactggtg aaaatccttt gatcggtccg aatgaagatg
    34561 caatgggccc tagatttcca gatatgagtc aagcttatac gcctgactat caagcaacag
    34621 caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat atgggctatt
    34681 ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc tcggacaata ggagccgatg
    34741 cagtagggat gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt
    34801 taggaatctc ttgtattacc aacttagcag caggtatgca agcgaatctg agccatgaag
    34861 aagttgtaga aacaactcag agagtcaaac aatcttttaa agcattggtt aaagaaacat
    34921 tgacattatt gtaataggag gaacaataaa atgagtgtgc atattgaagc gaaagaaggc
    34981 gagatcgccg ataagatttt acttccggga gatcctttgc gagctaaata tattgcggag
    35041 acttttttgg aagatccagt ttgttacaat caggtacgcg gtatgttagg ctttaccggc
    35101 aaatataaag gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc
    35161 atctatgccc atgaattgat ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca
    35221 tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca aggtgcagcg
    35281 accagttctt cgatgatcga aaaaaatttc caagcctttc attttcctcc aatcagtgat
    35341 ttcaatctat tactgaaagc atatgagata gctaaagaaa agggctatac ggttcatgta
    35401 ggaaatgttc tttcggaaga ttctttctac aaagatgatt tgacagagac gttccaatta
    35461 gctgaacttg gcgttttagg agtggaaatg gaagcagcgg ctctttatta cttaggcgcg
    35521 aaataccatg tgcagacatt aagtttgatg accgttagcg accatttgat caccggtgaa
    35581 gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa
    35641 acagcgatag cagaataaga aggaaatgaa aaagctgccc tgcattaagt aataagaaca
    35701 tccaagcaaa aatagctcct catgtttttg acttggctgg acttattatt gcaaatgtaa
    35761 gcttttgaac accgtttacc aagaggttgt gaa
//
LOCUS       DACCCM010000019        35719 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCCM010000019 DACCCM010000000
VERSION     DACCCM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.020"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..705)
                     /locus_tag="IVK78_000990"
     CDS             complement(<1..705)
                     /locus_tag="IVK78_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6374477.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="IVK78_000991"
     CDS             1393..1551
                     /locus_tag="IVK78_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6374478.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2849
                     /locus_tag="IVK78_000992"
     CDS             1623..2849
                     /locus_tag="IVK78_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6374479.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="IVK78_000993"
     CDS             complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="IVK78_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6374480.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="IVK78_000994"
     CDS             complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="IVK78_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP6374481.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4308..4775)
                     /locus_tag="IVK78_000995"
     CDS             complement(4308..4775)
                     /locus_tag="IVK78_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP6374482.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4895..6433)
                     /locus_tag="IVK78_000996"
     CDS             complement(4895..6433)
                     /locus_tag="IVK78_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP6374483.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6513..7199
                     /locus_tag="IVK78_000997"
     CDS             6513..7199
                     /locus_tag="IVK78_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP6374484.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="IVK78_000998"
     CDS             complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="IVK78_000998"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6374485.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="IVK78_000999"
     CDS             complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="IVK78_000999"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6374486.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8685..9815)
                     /locus_tag="IVK78_001000"
     CDS             complement(8685..9815)
                     /locus_tag="IVK78_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6374487.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9815..11998)
                     /locus_tag="IVK78_001001"
     CDS             complement(9815..11998)
                     /locus_tag="IVK78_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6374488.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11995..13020)
                     /locus_tag="IVK78_001002"
     CDS             complement(11995..13020)
                     /locus_tag="IVK78_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6374489.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13065..13892)
                     /locus_tag="IVK78_001003"
     CDS             complement(13065..13892)
                     /locus_tag="IVK78_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6374490.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13889..14956)
                     /locus_tag="IVK78_001004"
     CDS             complement(13889..14956)
                     /locus_tag="IVK78_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP6374491.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14982..15818)
                     /locus_tag="IVK78_001005"
     CDS             complement(14982..15818)
                     /locus_tag="IVK78_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6374492.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15815..16684)
                     /locus_tag="IVK78_001006"
     CDS             complement(15815..16684)
                     /locus_tag="IVK78_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6374493.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16754..18037)
                     /locus_tag="IVK78_001007"
     CDS             complement(16754..18037)
                     /locus_tag="IVK78_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6374494.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18051..19736)
                     /locus_tag="IVK78_001008"
     CDS             complement(18051..19736)
                     /locus_tag="IVK78_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP6374495.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20011..21036
                     /locus_tag="IVK78_001009"
     CDS             20011..21036
                     /locus_tag="IVK78_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6374496.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21631..25284)
                     /gene="rpoC"
                     /locus_tag="IVK78_001010"
     CDS             complement(21631..25284)
                     /gene="rpoC"
                     /locus_tag="IVK78_001010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP6374497.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="IVK78_001011"
     CDS             complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="IVK78_001011"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6374498.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29708..30676
                     /locus_tag="IVK78_001012"
     CDS             29708..30676
                     /locus_tag="IVK78_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6374499.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30694..31599
                     /locus_tag="IVK78_001013"
     CDS             30694..31599
                     /locus_tag="IVK78_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374500.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31596..32402
                     /locus_tag="IVK78_001014"
     CDS             31596..32402
                     /locus_tag="IVK78_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374501.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32559..33527
                     /locus_tag="IVK78_001015"
     CDS             32559..33527
                     /locus_tag="IVK78_001015"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP6374502.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33844..34368)
                     /locus_tag="IVK78_001016"
     CDS             complement(33844..34368)
                     /locus_tag="IVK78_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP6374503.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34655..>35719
                     /gene="scm"
                     /locus_tag="IVK78_001017"
     CDS             34655..>35719
                     /gene="scm"
                     /locus_tag="IVK78_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6374504.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        10272 a         7539 c         6612 g        11296 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgcttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctagtgc tttaacacac tcagatattt
     2761 ctacaacgag aatatacgta aatacaccag atgtagtaga taaaactgtt tttgaagcct
     2821 ttcagagagg attgaataaa tgtgattagt atgtgttctt aaatgtgttc ttaaacaaga
     2881 aaaagacact tttcaaacaa ttgaaaagtg ccttgaaaca ttgatatata aataatatta
     2941 acgtttagag aattgagatg ctttacgagc ctttttgaga cctggttttt tacgttcgac
     3001 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     3061 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     3121 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     3181 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     3241 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     3301 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     3361 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     3421 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     3481 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     3541 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     3601 cactttgtct gcattgatga cgatgacgaa atcaccagta tccaaatgag gtgtgaaagt
     3661 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     3721 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     3781 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     3841 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     3901 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     3961 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     4021 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     4081 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     4141 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     4201 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     4261 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     4321 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     4381 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     4441 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     4501 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     4561 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     4621 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     4681 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     4741 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     4801 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     4861 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     4921 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     4981 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     5041 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     5101 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     5161 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     5221 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     5281 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     5341 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     5401 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     5461 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     5521 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     5581 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     5641 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     5701 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     5761 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     5821 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     5881 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     5941 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     6001 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     6061 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     6121 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     6181 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     6241 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     6301 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     6361 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     6421 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     6481 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     6541 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     6601 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     6661 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     6721 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     6781 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     6841 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     6901 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     6961 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     7021 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     7081 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     7141 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     7201 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     7261 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     7321 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     7381 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     7441 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     7501 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     7561 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     7621 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     7681 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     7741 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     7801 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     7861 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     7921 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     7981 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     8041 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     8101 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     8161 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     8221 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     8281 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     8341 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     8401 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     8461 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     8521 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     8581 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     8641 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     8701 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     8761 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     8821 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     8881 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     8941 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     9001 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     9061 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     9121 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     9181 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     9241 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     9301 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     9361 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     9421 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     9481 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     9541 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     9601 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     9661 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     9721 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     9781 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     9841 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     9901 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     9961 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    10021 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    10081 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    10141 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    10201 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    10261 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    10321 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    10381 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    10441 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    10501 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    10561 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    10621 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    10681 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    10741 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    10801 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    10861 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    10921 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    10981 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    11041 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    11101 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    11161 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    11221 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    11281 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    11341 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    11401 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    11461 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    11521 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    11581 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    11641 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    11701 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    11761 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    11821 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    11881 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    11941 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    12001 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    12061 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    12121 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    12181 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    12241 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    12301 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    12361 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    12421 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    12481 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    12541 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    12601 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    12661 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    12721 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    12781 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    12841 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    12901 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    12961 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    13021 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    13081 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    13141 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    13201 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    13261 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    13321 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    13381 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    13441 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    13501 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    13561 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    13621 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    13681 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    13741 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    13801 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    13861 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    13921 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    13981 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    14041 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    14101 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    14161 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    14221 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    14281 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    14341 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    14401 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    14461 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    14521 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    14581 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    14641 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    14701 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    14761 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    14821 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    14881 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    14941 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    15001 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    15061 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    15121 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    15181 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    15241 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    15301 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    15361 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    15421 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    15481 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    15541 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    15601 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    15661 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    15721 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    15781 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    15841 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    15901 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    15961 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    16021 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    16081 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    16141 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    16201 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    16261 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    16321 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    16381 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    16441 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    16501 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    16561 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    16621 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    16681 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    16741 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    16801 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    16861 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    16921 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    16981 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    17041 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    17101 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    17161 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    17221 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    17281 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    17341 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    17401 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    17461 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    17521 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    17581 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    17641 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    17701 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    17761 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    17821 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    17881 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    17941 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    18001 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    18061 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    18121 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    18181 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    18241 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    18301 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    18361 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    18421 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    18481 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    18541 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    18601 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    18661 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    18721 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    18781 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    18841 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    18901 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    18961 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    19021 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    19081 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    19141 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    19201 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    19261 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    19321 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    19381 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    19441 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    19501 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    19561 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    19621 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    19681 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    19741 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    19801 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    19861 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    19921 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    19981 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    20041 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    20101 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    20161 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    20221 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    20281 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    20341 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    20401 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    20461 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    20521 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    20581 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    20641 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    20701 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    20761 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    20821 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    20881 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    20941 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    21001 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    21061 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    21121 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    21181 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    21241 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    21301 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    21361 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    21421 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    21481 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    21541 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    21601 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    21661 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    21721 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    21781 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    21841 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    21901 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    21961 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    22021 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    22081 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    22141 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    22201 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    22261 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    22321 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    22381 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    22441 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    22501 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    22561 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    22621 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    22681 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    22741 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    22801 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    22861 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    22921 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    22981 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    23041 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    23101 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    23161 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    23221 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    23281 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    23341 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    23401 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    23461 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    23521 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    23581 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    23641 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    23701 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    23761 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    23821 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    23881 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    23941 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    24001 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    24061 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    24121 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    24181 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    24241 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    24301 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    24361 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    24421 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    24481 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    24541 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    24601 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    24661 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    24721 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    24781 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    24841 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    24901 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    24961 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    25021 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    25081 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    25141 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    25201 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    25261 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    25321 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    25381 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    25441 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    25501 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    25561 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    25621 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    25681 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    25741 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    25801 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    25861 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    25921 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    25981 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    26041 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    26101 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    26161 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    26221 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    26281 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    26341 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    26401 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    26461 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    26521 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    26581 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    26641 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    26701 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    26761 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    26821 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    26881 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    26941 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    27001 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    27061 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    27121 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    27181 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    27241 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    27301 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    27361 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    27421 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    27481 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    27541 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    27601 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    27661 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    27721 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    27781 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    27841 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    27901 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    27961 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    28021 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    28081 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    28141 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    28201 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    28261 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    28321 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    28381 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    28441 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    28501 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    28561 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    28621 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    28681 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    28741 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    28801 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    28861 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    28921 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    28981 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    29041 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    29101 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    29161 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    29221 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    29281 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    29341 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    29401 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    29461 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    29521 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    29581 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    29641 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    29701 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    29761 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    29821 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    29881 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    29941 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    30001 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    30061 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    30121 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    30181 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    30241 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    30301 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    30361 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    30421 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    30481 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    30541 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    30601 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    30661 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    30721 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    30781 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    30841 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    30901 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    30961 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    31021 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    31081 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    31141 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    31201 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    31261 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    31321 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    31381 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    31441 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    31501 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    31561 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    31621 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    31681 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    31741 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    31801 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    31861 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    31921 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    31981 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    32041 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    32101 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    32161 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    32221 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    32281 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    32341 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    32401 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    32461 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    32521 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    32581 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    32641 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    32701 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    32761 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    32821 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    32881 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    32941 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    33001 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    33061 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    33121 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    33181 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    33241 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    33301 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    33361 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    33421 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    33481 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    33541 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    33601 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    33661 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    33721 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    33781 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    33841 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    33901 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    33961 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    34021 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    34081 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    34141 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    34201 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    34261 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    34321 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    34381 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    34441 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    34501 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    34561 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    34621 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    34681 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    34741 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    34801 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    34861 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    34921 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    34981 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    35041 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    35101 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    35161 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    35221 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    35281 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    35341 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    35401 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    35461 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    35521 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    35581 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    35641 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    35701 tgatagctca gattcatca
//
LOCUS       DACCCM010000020        35178 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCCM010000020 DACCCM010000000
VERSION     DACCCM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.021"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            355..1173
                     /locus_tag="IVK78_001018"
     CDS             355..1173
                     /locus_tag="IVK78_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6374505.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1192..1599
                     /locus_tag="IVK78_001019"
     CDS             1192..1599
                     /locus_tag="IVK78_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374506.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            1919..3004
                     /locus_tag="IVK78_001020"
     CDS             1919..3004
                     /locus_tag="IVK78_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6374507.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            3840..4172
                     /locus_tag="IVK78_001021"
     CDS             3840..4172
                     /locus_tag="IVK78_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6374508.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4763..5092
                     /locus_tag="IVK78_001022"
     CDS             4763..5092
                     /locus_tag="IVK78_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374509.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            5079..5420
                     /locus_tag="IVK78_001023"
     CDS             5079..5420
                     /locus_tag="IVK78_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6374510.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5534..5773
                     /locus_tag="IVK78_001024"
     CDS             5534..5773
                     /locus_tag="IVK78_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374511.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5840..6079
                     /locus_tag="IVK78_001025"
     CDS             5840..6079
                     /locus_tag="IVK78_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374512.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            6206..6895
                     /locus_tag="IVK78_001026"
     CDS             6206..6895
                     /locus_tag="IVK78_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP6374513.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            6901..8067
                     /locus_tag="IVK78_001027"
     CDS             6901..8067
                     /locus_tag="IVK78_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP6374514.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            8149..8820
                     /locus_tag="IVK78_001028"
     CDS             8149..8820
                     /locus_tag="IVK78_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6374515.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8873..10849
                     /locus_tag="IVK78_001029"
     CDS             8873..10849
                     /locus_tag="IVK78_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6374516.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            10862..11614
                     /locus_tag="IVK78_001030"
     CDS             10862..11614
                     /locus_tag="IVK78_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6374517.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11630..12385
                     /locus_tag="IVK78_001031"
     CDS             11630..12385
                     /locus_tag="IVK78_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374518.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            12398..12658
                     /locus_tag="IVK78_001032"
     CDS             12398..12658
                     /locus_tag="IVK78_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374519.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12655..14745
                     /locus_tag="IVK78_001033"
     CDS             12655..14745
                     /locus_tag="IVK78_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6374520.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            14772..15236
                     /locus_tag="IVK78_001034"
     CDS             14772..15236
                     /locus_tag="IVK78_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374521.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            15233..15475
                     /locus_tag="IVK78_001035"
     CDS             15233..15475
                     /locus_tag="IVK78_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374522.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            15493..15795
                     /locus_tag="IVK78_001036"
     CDS             15493..15795
                     /locus_tag="IVK78_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374523.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            15865..17919
                     /locus_tag="IVK78_001037"
     CDS             15865..17919
                     /locus_tag="IVK78_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374524.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHEGRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYSTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYKIYQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFSGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            17919..20528
                     /locus_tag="IVK78_001038"
     CDS             17919..20528
                     /locus_tag="IVK78_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP6374525.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            20525..23056
                     /locus_tag="IVK78_001039"
     CDS             20525..23056
                     /locus_tag="IVK78_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374526.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            23108..23440
                     /locus_tag="IVK78_001040"
     CDS             23108..23440
                     /locus_tag="IVK78_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374527.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            23441..24034
                     /locus_tag="IVK78_001041"
     CDS             23441..24034
                     /locus_tag="IVK78_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374528.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            24197..25891
                     /locus_tag="IVK78_001042"
     CDS             24197..25891
                     /locus_tag="IVK78_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6374529.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKCKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            26579..28456
                     /gene="ltrA"
                     /locus_tag="IVK78_001043"
     CDS             26579..28456
                     /gene="ltrA"
                     /locus_tag="IVK78_001043"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6374530.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            28587..28964
                     /locus_tag="IVK78_001044"
                     /pseudo
     CDS             28587..28964
                     /locus_tag="IVK78_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            28964..30169
                     /locus_tag="IVK78_001045"
     CDS             28964..30169
                     /locus_tag="IVK78_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6374531.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            30185..30829
                     /locus_tag="IVK78_001046"
     CDS             30185..30829
                     /locus_tag="IVK78_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374532.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            30840..31646
                     /locus_tag="IVK78_001047"
     CDS             30840..31646
                     /locus_tag="IVK78_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374533.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKEKI"
     gene            31668..31898
                     /locus_tag="IVK78_001048"
     CDS             31668..31898
                     /locus_tag="IVK78_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374534.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            32399..32695
                     /locus_tag="IVK78_001049"
     CDS             32399..32695
                     /locus_tag="IVK78_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374535.1"
                     /translation="MDLIIRNVNAKFITEIERRCVELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTMILEEYVSQNNRIIEILIEQNKGSEL"
     gene            32697..33119
                     /locus_tag="IVK78_001050"
     CDS             32697..33119
                     /locus_tag="IVK78_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374536.1"
                     /translation="MAKVIDKHIYFYQTHIDILDEIISTHPEVKNYSEAVRFLCMNYD
                     PEMEKKVNANQAKLNSMSKEISTMTEIIAEFADIHLKDAGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTQFLKR"
     gene            33138..34730
                     /locus_tag="IVK78_001051"
     CDS             33138..34730
                     /locus_tag="IVK78_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374537.1"
                     /translation="MTQSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKSVLNDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTHSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSISSDEV
                     KGSDLKEIKQIFQKGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLTEVYKTLPDKRSEWNYGNENRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKKEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIVSVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERH"
     gene            34742..35077
                     /locus_tag="IVK78_001052"
     CDS             34742..35077
                     /locus_tag="IVK78_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374538.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYESKLVPYYTTWEMNEEGINNFPCESI
                     KESISLTEC"
BASE COUNT        13083 a         5362 c         7059 g         9674 t
ORIGIN      
        1 gtatatgatc taagtcttgc ttctagtatg aatatgaccg atgtatacga aaagttatta
       61 atactagaaa atgaatatga tcaattcaat tcaaatcagc agcgaaaagt aaagaaacag
      121 atagactaga atagctattt tcgaaaagca atatttaagg tataagttat acctttaata
      181 ataaaagtat aagttatacc ataaatatat atggtataac ttatactttt attattaaaa
      241 accgctatat ccaaacgttt ttagatatta ttatttaagt tatactttta atgttgacca
      301 tttccgatat ataagttata ctttaattat caattaaaga ttgggaggat ttacatggat
      361 acttttcaag ggactacatt tacagttggc aattttaaag gaggagtagg taaaactaag
      421 atagtcagca tgcttgctta tgacaatgca atgataagaa atagaaaaac gcttattatt
      481 gatttagacc ctcaagcgaa cttaacacaa gtcatagcaa gaactttcgg tataaccgat
      541 atcaaaacca ctataacaaa tggtgtcgca aatggagaat tagcaagtag tattattaag
      601 gtaaatgaaa atcttgacct tctggcatgt gacactagtt ttcgatcatt ctccgctttt
      661 gctaataaac atgaaaatga attagaccaa gttactgtct tgaaaaaatt acttcttcca
      721 ataaagaaca attatcaaga aatatttatt gatgtaccac caaccattag cgaattttcc
      781 gataatgcta tggcagcttc agactattcg ataatttcat ttcaaacagc tgaagaatcc
      841 ttagagggag tcaacaaata tgtaaattat caaaaattca tggtcgatag atacgatctt
      901 gatttgcaaa ttatagatat tgtaccttgt atggtagaac caaatgatga ttttgatgaa
      961 gaaatcctag aagaagcaat tgaaaaatac ggaaccgtgg tatccaaaaa tctcattcat
     1021 tatcaaaaaa gattgagaag gtattctaaa attggtatac atttaagaaa atacaaaaat
     1081 ggaaattttg atcaatggga ttataaagca caccaagtat ttataaatat tttggcagaa
     1141 ctagatgcta gacaagagtt tttaaataaa taataaggag ttgcataagt aatgcctaaa
     1201 gattttgata ttcctaaaat gatggagaat ggtgaatttg agccattatt acccaataac
     1261 aaacgccaag atgtgaaaat aagcggtgaa tataacttta gcaatagtgc attgagtaac
     1321 acaagaaata ttacattgaa aaaaagaggc cgaccagttt caattcctga taaaagaaac
     1381 aaggtatcag ttcctaaaaa gatctctccg gcattaaata gcaaactctc aattttacaa
     1441 gactatatga cggagttaca aagtgaaaaa gggagaattt ctttcgaaaa aattgttaat
     1501 acattagcag actcatatat ccagcataga ctaggagtag caaaagaaga acacataaga
     1561 gaagaaatac aagaagagtt tgaaaaatta ccaaaataat tttttttcat gcggtattca
     1621 attaaagtat aagttatact agataaatga atattaaagt ataagttata cattaaatat
     1681 ttaaggtata acttaataaa caacgtgtaa atttgcgatt aaatcaacat tataaaatta
     1741 ttatttaaag tataagttat acattatttg tatttaaagt ataacttata cttatttttt
     1801 taatatataa cttatacctt aaatactgaa agtgttttat tcacccttaa tacatggtat
     1861 agttcacttg ttgaaaatcc tgaatatata caagtttcta agaagaagga gaaatttaat
     1921 gtctacagaa aatcaatctc aaaaaatgat aattaaaaat tggggatata aagcaacgaa
     1981 tgtttatatt gaaaaatatt ataaatttcc tagtttttta attcagaatt ctaaatactc
     2041 taaaatgaaa gacagctcaa aaatagccta tatgcttttt aaaaatgaat ttcgtagagc
     2101 cttacatgaa aaatggatcg acgatgatgg tctattatat ttggaattta cacaagcaga
     2161 cctaatggac aaattaaatt gctaccaagg caaagtaaaa accattttaa acgagttaga
     2221 aacatataat ttgatttcag tcgtaaaagg agaatttaac ccaaaaacga aaaaaaatga
     2281 aaaaaataaa tactatttac ttcaacctga aataagtgcc gatgatcttt ttattcaaaa
     2341 ttctttcgat gacgatttaa gcgaaactat tgataaatca acctcttctc ggaatgcgga
     2401 aatcgcacgt cgagaggaaa ctgctaaaac ccttgataac actggaaatg cggaaatcgc
     2461 acgtcgagaa aaaacagctg aaacccttga taacactgga aatgcggaaa tcgcacaaga
     2521 ttattatatt aataaatctt tagacactaa aagacactta aaagacactg aaacagacca
     2581 attacaaaat caagttttgc tagataactt cgttgaaatc atgaaagaag acagcataaa
     2641 cacatttgta ccagaaaagg tattaagctt gataaaaaca ttttctaaca cttacgaaga
     2701 agcccaacaa accgtcacca caatacacaa agcaaaacat aaagccgagg aacttgagaa
     2761 gacggtagtt gtgtatgaag aacttgaatc ttacggaatt gatgcggata aaggattata
     2821 tatgacgttg ctgaaagctt atcaaaaaca aaagactgag aaagtaaata atttgcagaa
     2881 tttgattttt gtgtatgtaa aaaactggtt tattgaaaaa gctattgcgg caaagcttgc
     2941 aaacgaacaa agagaaacat ttaacgaatt accaacagtt agtacggaaa attggttaga
     3001 gtagttgata aaaagcaatt actataaaaa tgatcaattt taaattagaa cttttttatg
     3061 attttaattt tattacaaat attacaaata aatagttagt attccggagt ttttagatta
     3121 aaagtttatg aaaacaatca caatcgtagg ttttaaattc ataatcgtta gatattttag
     3181 ttatttttca ctcaaaaaaa tagggaacta gtgtttgcat atgtgctttt ttgagttata
     3241 ataattcatg aagagaggta attcacttat ttccccaagc tgaaaagcct gatattactt
     3301 cttttcaggc agtgctgcta atattcaatt acactttttt gtatattttt agttgtttta
     3361 aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggacgata
     3421 aatttggaca attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctacca
     3481 accttcgtcg acgtccacag gtggcattaa gatcactccc ttaatgcgtt tacctttcag
     3541 tgttacggta ttacccgtaa cggatttagt ataacagata ttcaaaagtg ataccacttt
     3601 ttttggctgt gttaaggtta aaaatacgtg atattacgat gaatctatgc ctaagcataa
     3661 tgttataaaa atgtccgtgt ggctctttgc cgtatgacga acgctgaaat tagagggaac
     3721 gcatgtttgg atgcgttccc tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt
     3781 ttgaaaaata acaagaaaaa cttgttaaaa atatttaaag taagtgggtg gattatttta
     3841 tgtcatatac aggaattttg agtctcgaag atatttgtca ctacggtaaa cggtgtaccg
     3901 ctacagaaaa gatcacgaag aaactatcaa ctggtcaaag taaaaccgtt gtgcagtgta
     3961 agaaatatat aatccagaaa gacaaagtct cagaagaaat gatttattac attgggaaac
     4021 aaaaacaaat aattttaaaa gatcctatcc ctttaaaaga gctatatcca acaatcaaac
     4081 atgtttatga ccaaaacggt gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta
     4141 ccgcaaaagg gatggggcgt ttaatcagct gaattgatca ttctcataac ttgtatttta
     4201 tcgtttttcg cttgcttgaa aatattgtcg agacttctaa aggaacttag aatcactaga
     4261 aaaataagga gtagacttga tctatcaaca attcccttcg gggagtgacg gggtaggttt
     4321 ttacgattgg taacgtaaaa aattggtcaa tcgagtaaat gcccttgttt ggatagtggt
     4381 gaaatgaatt tgtgtaaata atatgcacag agtaaacaag agggaattta gaagttagac
     4441 aagttagaat agcgcctaga ttaatcatga gcagctacta agggatctag gctttgacgc
     4501 aaactcatgg aataaggagt aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt
     4561 cgtgtgctgc agtacatgta gatacttgcg aggacgttga tcattggatc aatcgagcat
     4621 ttaccgctgt ctactagggt ttctcgtttc ggacgccata aacgagtcga tttattttat
     4681 atatttttga gtgaacgaaa aacgataaag atattcgtta tgaagtaaaa ctaatttagt
     4741 agatagcaaa ggtggtagag gaatgattca aaaatttgcg aaaatgagta aaactgcatg
     4801 gattgaaaag cttgaaaatg acttgagtgt catgcgatta gctccaaaga aattcaaacg
     4861 agagattgca gaaaaagaag caattttgtc ggtattgaat gcggtagacg ataaatgtcc
     4921 agtaaaaatt agtttttcag atatagcaac aattatcgaa ggtatttatt atggtgaagt
     4981 tgatgaagag atatcttcta caaaagattt attagatttt ttggataaaa acaaagatac
     5041 ctttatagta ctaactgatt atgaggaggt tacaacaaat gaaaaattat gatccgaata
     5101 tacgattggg cacacatacg attaaggttt ctttccaacg ttgggattac aaaggatttg
     5161 tgacgtttag aagaggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg
     5221 atttatacga tcaaaaattg actgataatc caattggatt cggcttgcta cctgaagatg
     5281 atgaaggtaa tgaatggttt aaaatgacgc ttacggatga taatggagac gaattatctg
     5341 ttgaagatac ttggtcatat ttgagtgatt atattgtcag cgttgaaatc attgaatttg
     5401 tagcggataa agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg
     5461 agtcacgacg aataataaaa aggtagtatt accttaggca ttgagtaagg aatttattta
     5521 ggaggaaaat tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa
     5581 aggaaggtat gtacagctgt tagctcgtgg ttattcacct aaagatgtat tcaaatccct
     5641 gtctaaagga acagatatgg aaaaagaaag attacgaaaa gaattcgatt attggagaac
     5701 ccataatgga atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat
     5761 aacccaagac tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac
     5821 aagaaaggta ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa
     5881 agaaatggaa gaaaagttgc taaaaatgat gaaacaaaaa caccttaaaa gattatctgt
     5941 catgcaatat atcaatgata tgaagataac aggaaaagag aaagcttgtt tgttaggttc
     6001 aatgaaaaac tttgaacagt tgaggcgaac ttatgtaaaa acaggttcca attgccaact
     6061 attattagaa gtttcttgac gattatgata tgaaaatgat caattatgat gtggcattgt
     6121 tattgaataa aaagaatagt aaaatttaac atggtttatc ttggttagat gtaggtaaat
     6181 taattcatat atagaaggta gatagatgaa taaaaacaat aaaactttat ttaatcctta
     6241 cgtgggcgta atcattgttt ttcttctttt tagttatttt gcttatcaag gttatcaaaa
     6301 ggacaaaatc gctgaaaaag tagatggtgt aacaaaaaca atccatattc aggaacaggc
     6361 taaaacctat caagataatt taaccagttt aacggatatt tctaagagac aactgattac
     6421 taatcaaaaa caggcattag aaaaggatct caaaggtgaa attgctggaa aacttgtttt
     6481 ttcttctaca ggatttattc tccctgtttt tcaaggagcg aatagcgaca cgcttagttt
     6541 aggagtagct tctacttatt atctagatac tgaaatggga aatggaaatt atattcttgc
     6601 tggttataac gtagatacac caagcgtttt attatccgat atgactgatt tatctgaagg
     6661 agaagaaatt gatctaatcg atgctcatta caagtatcag tatgaagtct ccaaaagagg
     6721 aaaaatttct gattacagcc aggtagttca atctaaggaa aacaattcct ttttatcctt
     6781 gccaaaagca ggtaaaaaac cactactcac actgttagcc tttcaagcaa ataatatgaa
     6841 aaagtgcgat gtgatccagt gtgaattagt caatgtagaa aaaatcaata aataagggaa
     6901 gtgagtcaca ttcaagaact tgtagaaaaa ttgatcaata aaatgagtgc catttttctg
     6961 ttactgttgg tatccattct atatttaata tggacggtgg ggttaaatcc tcctacgata
     7021 ttatttattt atgataatgc tgcacagtta gtgacgtttt tcgtaggctg tttgtttctt
     7081 ttgaatctgt acaaggtaaa gagaaacgat ctattcttca ttgcagcagg cattcttttt
     7141 taccttttct tttctttttt tagaagctac cgaggagata ctttctatgg tgatctgatc
     7201 gttccgacaa ttattgctat tattttgatt gtaaaatgga cgaaatttac tgtaattgat
     7261 cgtagcttgt atcttgtagt attctatgtc tttttactcg taacggttta tcgagtattt
     7321 actgagatac cagtaccaga aggacaaagt atttggaatc catcgaacaa attatcagat
     7381 atttggatca atacaaatac gataggtagt tctttaatga cattagccct tcttatcaca
     7441 ggatttgcta gttcatttgg aaaatggtac atgcgattgc tatcattacc agcgttgatt
     7501 cttgctgggg caacgatttg ggtttgccaa tcgaaagcag cattatatgc gcttgttatc
     7561 ttcattatct tagaaatttt acctaagaag gtttttcgaa atacacactt gtccttttgg
     7621 atgtatgcag cagtttcggt tctagcttta ccgatttctt attttgctgc aatgtcagac
     7681 aaagttcact tatttacagg acgagaagaa atttggcgca aattctatga aacattaggc
     7741 gaaaaaacag gacaaatatt tgtagggatg aaacctttct ttttccatag aggaaatcag
     7801 attctaggta atcataatac gtatcacgca ctcgtcaatc actatggatt gattggtttt
     7861 gttctatcga ttattctttt gcttagtttc ttatggttgc tcgtaagaaa acgtgagctg
     7921 acaaatggac aactaacatt cttgtgggct tttttagcga tcatgtgtca atcgttcatg
     7981 gaagaaacat taacgacaat cagttgggtg ccaattgtat tcttaatgtt agcaatggct
     8041 acacaccaat ataaaaccac cagataaatg aaatagaata gtaataagag aaaacacttt
     8101 ttcgttgtga aaaagtgttt tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg
     8161 ttatttggtt ttttaggagt cgctttaatc gtcgtttgca gtgtgtttgg ctatgttagc
     8221 taccaaaaac atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat
     8281 caaatcaatg cacacgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg
     8341 tctttggggc aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa
     8401 gtatttgcac aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca
     8461 aatcaataca ctttgagtct tggagcggct acttattttt atgaagatgc cgaaatgggt
     8521 aaaggaaact atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat
     8581 attcaaaagt tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt
     8641 tacaaggtaa cgcgtaaatt tatcgtacct gagtacttta aattaatcga tggcgttcct
     8701 gaagagaact ctttcttgtc tttaccaaag aaaggagaga aacccttatt aacgttgttt
     8761 acttgtgttt atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa
     8821 caagaattta atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat
     8881 aaatcggtat gtaaaaaaag ctggaatgat tgttggaatg ttaggaatca ttgcaggctg
     8941 ctttcagggg tatcgagctt atgcagaaga tgtcacgcaa aaaacgccac cagaaagggt
     9001 caatatcacg gttcataaat taatgtacga tcaaagtacg caattgaacg ttgatattga
     9061 cggaatcaaa aatgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa
     9121 caaggcagat tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc
     9181 gactgaagat tctgatctaa ccaacgcgaa agtggacgaa atcgttaaag atgtagaaga
     9241 caagggatct aacagtgagt acgttaagaa tgcaaccaat aaaattactt cagctgtaga
     9301 tgaaaatggt gagatcactt ttgctgatca accagcctat gtcaattcca aagggaatgt
     9361 atatgtggtt tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat
     9421 ggttgtgatt gcgccaatga cagacaacac aggatcagga tttttaaaag atattcattt
     9481 gtatccaaag aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg
     9541 cacagctcaa agcaaacaaa cacctttaaa aggggcaaaa tttgagttat acaagggcga
     9601 acctggaaaa ggaacaaaat taggagagct tgtatcggat gatcaaggaa agctgactgc
     9661 aactgatttg acgttaggaa aatactactt tgtggaagtt ccttcagaag ttgtagttgg
     9721 atcagatcaa gaaccaacag cagaccaata tttgttaggc gcagacgctc gcaacgatgc
     9781 gcacaataag ttaacctttg aaattacgaa tgacggcgtg acaagtgatt taaaagcttc
     9841 ctacgtgaac tataaagcac cagtgatcga taaaacagtg acaaatggaa caggagaaga
     9901 acattctttc caaattggtg acgcagtgaa ttatcaaggt acaattcata tccctacaga
     9961 tatcgcaggt ggaacagatg ggattacagt aaatggtgta aaaagtgaaa cttctcctta
    10021 cagtgtattt aaatggggcg acacagcagg taaaggactt tcttatgttg ctgaaaaagc
    10081 agatattaaa gtaacaaata atgatggttc agtcgtatta aaagaaaata ctgactataa
    10141 aatcgaaaat tctgatagtg gttttgtgat cgattttatc gtaaataatg gacaagtaag
    10201 tgatacagtt gcgaatttac atggtcaaga tctaaaaatg acttataaca tgtttgtgaa
    10261 tgatagtgct gaaatcgcaa atccattaac aaatagcgtg gatttcgttt ataacaataa
    10321 tccatttaat caggaagaac accacgaaaa aacgaaagct gacgttgtga cttatggcgc
    10381 aaaattctta aaagtcgata gcggcctatt tggaacaggt atcaaagcaa caccgcttga
    10441 aggcgcagaa tttgcagcta agaatgcaga aggtaagtac tacggtggac ttactgatac
    10501 agataaagat ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg cagcaatcct
    10561 aaaatcagat aaagagggtc attttgaaat cactggttta actgaaggag attactcact
    10621 ggaagaaaca aaagctcctg aaaactatca aaaattaaca aaagagatct ctttcaaagt
    10681 agacaaagat tcatacaaag aagaaaaccg aatcacgatc aaaaataatc aaaaagcttc
    10741 cgtaccaatg actggtagca acggtttcca atcttacgta ttgatctctt gcttgttgct
    10801 tggggcaggc gcaatttctg cagttgttta ctttaagaag aaagcctaga aggatcagct
    10861 aatgattatc agacacccta agaaaaagcg aatcatgggt aaatggatca ttgctttctg
    10921 gttactttca gctgttggtg tcttactttt gatgcctgct gaagcgagcg tggcgaaata
    10981 ccaacaaaat cagcagattg ccgcaatcga tcgaacagga acggcagcag aaacggattc
    11041 ttctttggat gttgctaaga ttgagttagg cgatcctgtt ggtattctta caattccaag
    11101 tatttcttta aaactaccaa tttatgacgg aacttccgac aaaattttag agaatggtgt
    11161 agggattacg gaaggaactg gcgatattac aggaggaaac ggtaaaaatc ctttaatcgc
    11221 agggcacagt ggactttata aagataatct tttcgatgat ttgccttcag ttaaaaaggg
    11281 agaaaagttt tatatcaaag ttgatggtga gcagcacgct tatcaaatcg atcggattga
    11341 ggaagtgcaa aaagacgaac tacaaagaaa ctttgtcact tacttagaac caaatcccaa
    11401 tgaagatcga gtaacgctaa tgacatgtac gcctaagggg atcaacacac accgtttcct
    11461 agtgtatggg aagcgagtaa cctttacaaa atcagaatta aaagatgaag aaaataagaa
    11521 acaaaaatta tcttggaaat ggcttttagg aagtactgtt tttctttcag tcatgatcat
    11581 tggttcgcta tttgtttata aaaagaaaaa ataaaggagg gtaaccaaca tgagaaatag
    11641 aaaatggcta tttgtttgtg cagccgctct tttttctttc actgcctaca cacagttgac
    11701 gtttgcagac agacaagaaa gcacggatag cacagaacaa acaatccaaa cgtcagatag
    11761 cagcacgatt caaagcagcg atgaaaccgt cgattcgtca acagtggatt cttcagaaga
    11821 accgaaaatt gctgaagacg atcaaatagt caaacagacc attcacgttc aaaagattat
    11881 ttcttcagaa caattagaca accaggggca tgaaaaacaa gaaggagtaa atggcgcgaa
    11941 gttttctgtt tatgatgtat cggacatttt acaaaagatg gatgttaagg atctcactac
    12001 tgaccaaatt gaatcccaat taaaagatcg agtgaaaaaa ctatccagtg atcaattgaa
    12061 gctagtttct aatggagaaa caaagacaat tgatcaacaa acaggtgtct ttgaattctc
    12121 ggtagaagta caggcaaatc agaaacaagc ttattatatt gtcaatgaat caagtcctga
    12181 aaacatttct aatagcgagg atattttgct attaacacct gtttctgaaa aaaacggtga
    12241 attcttaaag gatgtgtgga tttatcctaa atcagaagct tcgcaaccta aagaagaagt
    12301 aaaaaaaatc gtttctactg gtgtaaagaa aaacttcttt gagaattgtt gggattttgt
    12361 tactcacttg ttttctagag attaaggaag aaataagatg aaactgatca aattattatt
    12421 attcaatctt tcttgtttaa ccgcagtctt tttgtactgc ggtttattat tttctcaatt
    12481 atctattttg ccaagctacc aactatacct aatgaggatt ttaaacatac cagctgcttc
    12541 catgccgcta gatattatgg aaaagctcat taggaatcca ttatttacta cgatcaattt
    12601 gatcgttatt gtcggaattt cactagtcat ttataaaaaa gaggggatga agggatgaat
    12661 tttaaacact cgtggaccag acggttaaaa tatgtttttc ccataatgat gatgttaggt
    12721 acattgtttg ggttaaaaac agccaatgtc tttgctgaaa aagttattgt cgatccgtca
    12781 aatccaatcc agaacgtaaa gaataatttt attattccaa aagatgttcc taatgcaaaa
    12841 acaaatatta ctgtaaacgg agcgtccaaa gtttacaaat atcatgttga tgccaacaaa
    12901 ggtggtttta cagacgacta cgtaggatta gtggagggaa acaaaaatat acgctatcct
    12961 tctttccaaa aggaatacgg tgaaacaaac cttgttttag aaggcgtaac aaccaacacc
    13021 aaagtcaata ttgactatgg aaaaatcggt acgtataacg ggaaaaaagt caatatcaag
    13081 ctagttctat ctaatattca cttatactcg gatacgttgc cgtggaatct actagataac
    13141 aactatacaa aaactcattt tcgagacgat ggctataaaa atacgaatgg tgcaatgagt
    13201 aaatctaaaa aaagaacagt actttggatc tcagacaatc ttttctcagg aattgtttac
    13261 cattcaacac aaatgaatgt gcagctagta gccacatatg aggacggtag ccccgttcaa
    13321 ttttcaggag atacatttat cagttttaac tctttgaacc ctgcaggagg aaaaagcacg
    13381 gatcttaaag gagaatatgc ccactatgac aaaatgaata cgacagattg gtatgtcaga
    13441 caagatgcgg ttctttcaga atttaaatct ttctacaaca atctgaacgt ggtaggtggc
    13501 cacccaggag gaagctctaa gctgacacaa gcggataatg atttcaataa tttgcatgat
    13561 aaattaggag atccgaagtt tggtcaaggt actgtatcct ttaaaatatc tgaagcaaat
    13621 ccgacgtttg tgattggttc ttctaatgtt caaacctggt ttactttatc ttctgcgaca
    13681 atctttagtg ttgttcctga tcaacctgaa aaaacagggg ttgataaaaa tggaaataac
    13741 gtgaacgata aaatgttgca agtaggagac acgattcaat atcgaatcaa acagaaggtg
    13801 aatcgattag gagtggattt actagcggtt tacgatcgat ttgagttaat cgataattta
    13861 ccaaaagaag tagattacgt cgatgcacac gttgaaagtg gtacaaataa aaaattcgat
    13921 gtatctggtg aagtgaccta tgacaaaaca aaacaccagg taaaatatgc agcgaaagct
    13981 gatacattaa aaaatcgaat gaagtataac ggcgaaactt acgaattagt gatcaatgta
    14041 aaagtcaatg aattggccaa tcaaaatagt gtagccaaaa atcagggaac gtcgatcatt
    14101 aataaagtag aaaaggatac aaatatcatt accgtttatt tccctaaaat tccagtgaaa
    14161 gaagtacaac aaaatggaaa agatgtaaac ggacgaaatg acggtaagaa gggtacgccg
    14221 acagctcctt taaacgcagg atctgaagta caatatctag tcacacaaaa atggcacaca
    14281 aaaggcgtag acgctgcgtc agatcactac aaacaattta gtattcaaga tccaattgaa
    14341 gctcgtttga catataaaga aggttctgcg caagtaatag ataagagtaa aggaaaagat
    14401 attacttctg aaggcacact aacctatgat agcaattcaa gaacgctgaa gtgggaagct
    14461 tccgctgact tcttaagtaa caatctttta gatggacgag aaattcagct gatatttaca
    14521 gctaaaactc cattacaatc agaaaaaaat attgataacc aagccgtagt agcagtagat
    14581 aatgtttcta ataaaacaaa tgttgtgaca attggggttg atcctaactt gccacaggtc
    14641 attgttccta aaacaggttc tacacattta gtaacgattt cagcggtcag cttactatta
    14701 cttgtgttag caacttttag ttatgcggtt ctgagataca tttaaaataa aaaaacgaaa
    14761 gaatgaataa aatgaaaaag ttaataatag tagttacttc cttgattgcc atgattgtta
    14821 ttttagtcgg atgtagtaat gaaaaaaata aacaaactaa tcaagataat ggcgtattga
    14881 aatcaggaac tatgtggaaa gaagaagtag gtggtcttgt ttataatttg aaaattatag
    14941 acgagactac ctgggaatac tctgagagtg tatggcatcc agatcctgta caaataacag
    15001 tcaagaggca aaaagactat aaaggtttag aaagatacaa aatagtagat tcagctggtg
    15061 ttagggaatt tataaacaaa tctgattctt tatttattgt tgttccctat gagaagaatg
    15121 gcgtaaaaaa aataattttt cttggatcta gcaaggatga aaagcgaacg aaagagaaat
    15181 tgattcacga tggatcacaa tccaataaat ataagttaca aaagactagc gagtgattgc
    15241 tagtcttttt atttgtcctg aaggaggaat cacagtggca aaagctaaaa cgccatttga
    15301 aaaagccgcg tctaaaatct tagaacgccg cggtgtatct gaaacacagt ggaaacaaga
    15361 agtccttgaa aaagcacaat ttgacctatt acggggaaaa gacaaggact tagaaggata
    15421 tatctatcaa cgtgaacgtg aaaagcttgt tttcgatgaa attattaaac actaaaagga
    15481 gcgaacaatt ttatgtttga atcaattaca atgaattttt taacacaacc gaaagtagta
    15541 gctgcggttg atatttttag agcattacaa actgccgcaa ctagtttaaa aaacaatctg
    15601 atgatttttg ctgctgctgc ggccgttgct tccattgtta ttgggggtct tttcttccta
    15661 tttggacgaa gaggagctga aaatgggaag atgcacgtag gctatgtatt gatgggggta
    15721 ggagttattt tagcagcagc ttctattgtg gtttcgttat ttggattgtt tggacaaaca
    15781 cctcaaggat tataagaaag taaaaaagta ggagtaagaa gtagtcgata ctttttactc
    15841 cttttttatg aaaggagaat actcatgtac tttacgattg caggcaatac caaaacagat
    15901 aaagagttaa taaaaaaagc aacgaaggaa gtaggaaagc gtacttcttt tttaccgaaa
    15961 acaattaaat attctgtcat tgaaacggaa gaacagctac atgtgttagg gaaagtactg
    16021 gaacacgaag gtcgatcttc tgttaagttg actctcacgt tacatgacac agaaggaaaa
    16081 atagagaaag ctggtacaga atacgttttt ggcccactgg attatcaaat ttcagaaggt
    16141 ttctattcta cattgatgga tttaatccgc cttgatgtgt ttaaagaaac ggatgaaact
    16201 atgagtgtgg aagagtctta tcgaaacgaa aaacttttac aagttttaga agaagagtta
    16261 gagagcgacc aaagaaaagt gaatcaaggg agtgaagtcg aagtaaatga agaagtacca
    16321 tgggaacgac caatatttcc aaagaaggag tcaaaagaaa aagagacagt ctatgttgac
    16381 gaacaagctg atattccgac tcagaaatcg atatttgagg aagagaacga tgtgatgggt
    16441 caattggagg tagatccctt tgaagaaaca atggaggatg aagagacggc cgaagtagaa
    16501 gttgatccag ttgaagctaa tgaagagtca acatggactg aaggggattc tcaggaatat
    16561 attgaaccac ttatcagtga agaagtagaa aaaaacgaaa tagagataca aaaggcaact
    16621 gtatttgatt atattttgga tcaatatccg ccagagcatg aatggatgaa agaaaaaaac
    16681 aaattatttt taaatgagct ttattcgaaa tatcaattac cccaaaccta tcaagcattt
    16741 ttagagagta aagaagaatt aatctcacag ggtaaaggca cacttttaga acagttagag
    16801 tctgtccaat caacaaattg gcatgagctt gcacaagaag atttattagc cacctttgaa
    16861 gaacgtcaac aagaaacaga tcaagaaatt aatgagtatg cgacggaaca gtcgtctaat
    16921 tgggagaaag caaaagagga actaaataaa gaagaacagc gagtgattga agaagaagta
    16981 caaaaaatta aagagcgata cgaaaagaaa cgcgacaatg cgtattcctt gtgtgtagaa
    17041 aagatcaata attttactca gacgaatcaa gaagcgttat cgaaagaaaa agaaaacttg
    17101 ttaaaagaac gtataagaga acgaaaagaa gaattttatc aaggtttgcg tacagataag
    17161 ctaaagatta gcaaagaatt aaatgagcaa ctgaataagc tctatcaaat aacaagtgaa
    17221 gctcttatgg aaaaacacga gcagatccag caagaattac aagtacaaat tccaaaatgg
    17281 aaaaaagagt ataaaattta ccaacaagaa ttgaaagaac aagaagaaaa acaacggcaa
    17341 aaagaaaaag agcgtgaaca attagaatta aaaaaacgtg aacaggatct tgcggaacaa
    17401 aaaatggcga tagaaaaaca acggttagag caagaaaagg aaaaagaagc agctgctcgt
    17461 aaggaacgtc aagaacaaat ggatacattg cgacaagcac agttaaatta tctagcacaa
    17521 cctcaaccag tagctgctgt tcaaacgcca gtgaacaacc aagcaaattt aactccttca
    17581 ccacagcgtt cctttagcgg atggatggtg ggatgtattg caagtttaag cttgcttcta
    17641 ggtggaggaa cagtatttgc ttttaatcat atcaatcaat cttcacaggc tgattctgtg
    17701 gccacaacga cgtcaatcaa ggaagaagcc caagaacaag caagaaaaga gtatgaacaa
    17761 caattagaag aattattgaa tcgtaccaat cagtcaacaa cagagtcaag tatggaaaat
    17821 agcaaagaga gtgatcctag taaggctaaa gaaggatcaa gcgattcaac tgtacaatca
    17881 aacacttccg aaagttcaga atcaaatgag gtgaaataat gcgtgttaga gaaacaacga
    17941 gacgaaaaga caaacgattc aatttagaaa agaataggta tctttctcat ttctatcgct
    18001 atactccttc aggagaagag ctaccagaaa aacatgacaa acgtatttct caaaagattt
    18061 tttatagtgg cttagcttat cttcctttaa actatgttgt tacaagaata ccagaagcat
    18121 ttcatcgatt tgagattatt ccaggaagtt tacaaggagt gaatattttt cagttacaag
    18181 aaatgccgca gttattccat tttgattcaa ctggtttttg gaatcctttg accccaacaa
    18241 caagtttagg cacacgttta gttgtttcta cgatttgtgt tgttccagct gtacttatgg
    18301 gattgaaaat ggacatgtgg tttcgtcctc tagcagatgg gcaaaaagga gataaccgat
    18361 taaaaacgtt aaaggaaatc caacaaattt atccgcaaat accagaatca aagggaggtt
    18421 ttcctggtta tggcggaata ccaattactc attataaaaa gtactggtat attcagacgg
    18481 atacagtaaa tacttgtatt gttggtactt cccgttcagg gaaaggacag atagaagttt
    18541 tagcaaccat tgacaatttg tctcgtgcag aaagacaaag ttcaatggtt gttaatgatc
    18601 caaaaacaga attatatgtt gctagtaaag atgaactaga agctagaggg tatgatgtgt
    18661 atgcatttaa tatcaccgat ccactgcaat caatgagtga taatccattg gcactcatcg
    18721 tgaaatactg gaagcgagga gacatagaca cagcgacaca gttaaccaat actttcacga
    18781 atacgattta ttatgatgcc aacgcttcag acaataaata ctttaacgaa aatgcgcaaa
    18841 aagctgtcaa tgcgcttatt tttgcgttat tggaacaagc ggatcagaca ggggattact
    18901 ccaaattaac accgaataat ctcgtcgaac ttctttctga gattggtggt tttaattacc
    18961 aagatcctga taatgaattc aagcagctca atgctttaga tgaatttatg aatcagttac
    19021 cacctggaaa catagcaaaa aaacaatatg gggcaacaaa aattggttct gataaagcga
    19081 aaggcaacat tctatcgaca gcgatcacag gactaaatcc atttacactg acgaaaattg
    19141 cgaaaatgac ttctcaatca accattgatt ataagtccat tggttttccg aagtatttgg
    19201 atctcaaact acacgagagt ctgttaaacc agcgaattgt tgtagagttt tatcatcaag
    19261 ggaaaaaatt tcatgaggaa cgtgtcaaag taggttatag aggtttttgt gaaatcaact
    19321 ttgattgtca tttagagcag ggagatcatg tttgcttaaa atacgatcat caaggaaaag
    19381 aatatgttgc ttggtatgag ttttctcaaa gagtattgaa agacgaagag ggaaatatcg
    19441 tttatcgaaa aaaacgagga gaaacccatt taccagaatt agaaaaagaa gctgttctaa
    19501 cattagatga ggataaaagt aacttattag tagagtctat tcggatgcac tataccgata
    19561 aaccaatggc tatttttatg ctcacacctg attatgatcc atctaaccat atcattgtta
    19621 gtatttttct tagtcagttg tataaagaat tatctacgca atgtgttaag actaaaggag
    19681 ataaatgtca ccgtaggatt cattttattt tagatgaatt tgggaatatg ccaccaatgg
    19741 ataatatgga aggaatcatg acagtcacag ctggtagaaa catgttgtgg gatttgttta
    19801 tccaatccta tcagcaactt tatgcgaagt atggggaaca aaacgcagcg attatcaaag
    19861 aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc gaagaatttt
    19921 ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat tcattaggta
    19981 tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct gaaagaattt
    20041 ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa gatttgaaag
    20101 gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt ccttatgctt
    20161 atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac atacattgta
    20221 cacatgcgga cttggatctt aaggctttac aaattgatta cgattatttt ctacctgata
    20281 tctatgcacg tcgactttat cacaatatgc acgatcaaat agaagaggaa gctaaagaaa
    20341 acatttttat taaaatcgtt caatctttag acaactcaac ggcacaaaag ttacttattc
    20401 aattttatga agagaagcaa atagatcgtt tgcaagaata ccttgaaaaa ttgcgtcaaa
    20461 atgaagaaat tacagaggtt ataaggaaac gacttttgct agaagctgaa aaggcggtga
    20521 aagaatgatc attggaggaa tattttccgg cattgccgac tggtggtcag gcttatttgg
    20581 agatggggat gcagatccca aaaccgtaac gaaagtctta caagattatg gtaattatct
    20641 gcagtatcaa gatgtattca gttggatttg taatactttt ttatggggtt tgattaaagg
    20701 gttatataat ctcaatactt tactagaaaa aacaatttat caaagctttg atttaaaaga
    20761 cttattgaac gccgctgggg taaatgcact atttcaaagt ttgattagta aggtattggc
    20821 tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga gtaaacaccc
    20881 accaaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga tttttggtgg
    20941 tagttcttta atcgatgaag ggttaaatat cagtaaaagc ttttacggag acgtcactgc
    21001 agcaaataaa gcagataaaa cgggatcacc tgcttttcaa attattcaaa ataatgtgat
    21061 tgatgtgagt acgttactag aaaatgatcc gaacaaagta gaacacatac caacagacaa
    21121 aagaaatgct ctcacggcga aaaattttaa aacagcgaat atcaatggga ttattactcc
    21181 tgaacaagct aaaggtatgg caggtaatga taaaagtggg ttatcagata tacaaaagga
    21241 tcggttaaag gcattacaat accgattgga actagatgat aatgggaaag aaattcctgt
    21301 tcaaattagt gacgaaggct tagcaaaata tgtctatact tcagggtatc gtcgatacag
    21361 tagtaaacca ggtattattt tagctggtga atcttctttg gcagtagcct atttatttat
    21421 cctatttacg atcgttactt gcattattga gctggtattt aaaaaattct atttagtgat
    21481 tgccgcgtca acggactttg agacaggtca acggatgaaa agtgcgatag aagatatttc
    21541 tcaatccttc ttgttattgg cgtttacgat tttagaatta cgaatctata ccttaatgct
    21601 ttcaggtatc ggtgatttgc atgcggcagg aaagatcaat ggattcttgt atgtcgttgc
    21661 tttgattgta ctaaccattg cgctattcaa aggctctcaa tcagttacga aaattttcgg
    21721 cgttgataca agcctgaaaa acggcagtaa ttctcttctt agcatgtttg ccttaggaaa
    21781 tattgcgaaa aatgtgggta gcggagctaa aaaccttgca agtgctggta agagtggttt
    21841 aagccattta aatgatattc gtaaaaatgg atttggccat cgaaaagatg ttacccctga
    21901 taacggggaa gaacaatcag acgataaagc tacaactgta cctaatagta agaaaacagt
    21961 ttccgaaatg ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag gtcttggtta
    22021 cgtcaatgaa cgtgggttaa aaggaacagc tgaagatctt acagaaaaag ccaaagacac
    22081 cttagaagat aaattagacg gcacgagagc taaagaattg gctgaagctg tgaagaatcc
    22141 atccgaagca ctagaaaaac aaaaagaaaa agcttcagaa gtcaaagaag gactaaaaga
    22201 tgcgtatggt gaaggacaag tcaatgcagc agtgaaagct aatacaacaa ccgaaaaaga
    22261 aaatcaagaa ggtatagtga ttccttcagt caaagatcac caagaagcgt tgcactctaa
    22321 tgaaaattcg attgaagaga aaaattccat agaagaaaga aattcagaag aaggaaaaaa
    22381 tggacagtta ccaaaaactg atccagaaca atatcgctat aaacctaaca gtgaccttga
    22441 aaatgagaag cgaatttcag atttatcaaa agtaaatcaa gaagacaaag cagaaaatga
    22501 gggacagtta ccaaaaactg atccagaaca atatcggtat agatctgtta actccgaaaa
    22561 tgaaaaacgc atttcagatt taccaaaacg aaatcaagaa gtacaaacgg atgaggattc
    22621 catcgttctg ccaaatgata atggaatcga agttggaact gaaaaatcaa cgaaaattgg
    22681 tttacctaag gcacaaaatt taccaatgaa agatagtcgt ttgaatcaat tcaaggagaa
    22741 tgatcaaagt tttgttcatc aatcacctgc aggtactgtt caaatgaaac caagtcgttt
    22801 gcctgaaaat ggaaatctga caactgtttc ttacaattta tctgaaagtg gtgagacagg
    22861 gaatgcgtcc gttcaaaatg ttgtgacaag agaagctgca ccaaaaccta ttcaaaacgt
    22921 tcatacaggt tacaagccag caaatgtggc atttactgca ccaacaaaaa taaatgtgac
    22981 aagagaaaca aaaaacagga acaatgcatt gaaagaacaa ctattgaggg atatcaaaca
    23041 ggatatcaaa ccataaaagg cctacaagtt aggccttttt tctatgtatg aaaaggagaa
    23101 atcatttatg gataaatata gtaacatgaa aaaccctcca agatttagtg gagaatggaa
    23161 gtttttcgct tttttctttt tagatctatt aacgcttggt ggctgctttt tttgggcgaa
    23221 aatggtcaat aatcttgtcc acttaccact cttttttgca ctcttgaatt atggcttatg
    23281 gattgcgata gcgttagtga ttgtttggcg accctataat aacccaggtc aacgacaaat
    23341 ttttgcgatt atcgagtcta ttttttttgc ggacaagaac cattatcatg cgttagatcc
    23401 taaccgaaat tatcatggta cgtataagga ggatgaataa atgcgagtca aaggacaaaa
    23461 ttcagttgaa aggaaatcca ttttgcagtt tggaaaacca gaagagcgct ctatcttgga
    23521 cttatttccg tataaatcct gtacagaaga gggagtctta attactagag aagatcattt
    23581 tcaacgttat tatcgtgtag tttctacaga cgtggaagga ttaaatgaac aagagaaact
    23641 agaacgaatg aatcagttga ccaccgtcat gcgtacgtat gtgccaacaa ttaagttagt
    23701 tagtttgacc acagagacgg atctaacgga acaaatcgtg caaaagcggc aattattaga
    23761 taaaaatcgt atggaacagt ccttaggaag aaatctacaa cgattaaaaa aatatgaaaa
    23821 gaaattagtc gaagaaatcc aggaattgaa acgagcagaa caagaacgtc ctgacttaag
    23881 cttctttttc gtcattgaag gaaagacaat caaggattta caaaccagaa accgtcagtt
    23941 aatgcgatct agtggcattt tagggttaaa accactctac aaaaaagaat tgatcaagat
    24001 tctgtatcga atgaacaata tgaatgatga gtaggaatgg agaagagaga atgaaatatc
    24061 cgttgatatg gttagaaaaa gatgttccga aggaagagac cactagaaaa tttcctcaca
    24121 ctttaagcct gggtaagttg ggaaaagggc aatcttttaa ccaagtaaag aatcaaacaa
    24181 gaagaggtga ctgtacatgt tagaagcaat taaaacagct ttttcaccac aaaatgatca
    24241 aaaagtactt gatccattaa agcaactagg aaatcgggaa atagaaattt taaaatgcaa
    24301 agggtatgat ttagattttt taaaacaagt gatgccacaa ggtgggttaa attttgatga
    24361 agaatatgtg acgtatggta atggttattc acgatgtgtg acagtttatc actacacaaa
    24421 aaatcctacg cttttttggt tggccaatct catgaacaat cctcatacag tggccacact
    24481 cgatgttagc acagatgaaa agcaaaaagt gattgatcaa atcaatcgtg ccttagatga
    24541 attaaatgat cgatcagata atgaacgaaa agcgactgac cgaaataaaa gcgcggaaga
    24601 atacctggaa ctagcggaat atgctagaaa attgacttct aatggggaaa tttctaagca
    24661 agtcaaagtg agagtacatg tttacggaaa cacgattcaa gaagtagatg aacgagcgca
    24721 ggaactgatc gaaaatctca aagggatgga ttatcgtgcg gtgacttatc tgcataaaac
    24781 caaacgagat tggcaatcga tgtttaccat gttcgatgaa caagctgaat ggttcggaaa
    24841 tatcaaaggg caatcactac catcgttaaa cattggcgga ggtttcccgt tccaccacca
    24901 aacgctaaaa gatccaggag ggacacaata tggggaaacc atgacaggcg gcccatttgt
    24961 ctggaatgcg acgtataaaa atagtgtacg gttgtctact aataccttgc ttctaggaat
    25021 gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg gaagcccatt tagctgcagg
    25081 agactttatt tggggctttg aaaagggaaa agactttatt cctttcttaa aggaatataa
    25141 cggtattatg gtgcgtttag acggatctga cggaatgatc aatcctttag agatatttgc
    25201 cacacggacg tatgatgaag catccagttt atatgacgat ggatcagtca aagatttgaa
    25261 gatcaatgaa gcagcgtcct atcaaacaca cttagacaaa gtggtctatc aggtacagtt
    25321 agtgagtcct cagctaaaag gaacgatgaa agcagaattt aagacctact taaatcgttt
    25381 ttatgaagag tatggaacag taccacgcgg ctttacttcc tcaaattcgc gttcaaatac
    25441 tgagacgcaa gtcacaggga aagatccaga agcttatccg acgttcaaag aatttttaga
    25501 ctttttagga caactggaat taccaggtgc ttcccaagag aaaaagaatc gaaaagaaga
    25561 aatggaatcc atcgtagaat ccttatgtga gacgtatggc atgatttttg atggtcatag
    25621 tacgattcga caccttgacc aacaacagtt ggtctttttt gacattgact ctattacagg
    25681 cttagatcga ggggtacaac aatgtcaaat gtacatggct atgacgttga tttggaatca
    25741 ggcattgatt caaggaagaa gacaacgacg tttacttaaa gaaaaacaaa tggaagaaca
    25801 agacttcaaa cgcttcttgg cgatgattga tgagtgccat aatggtgcga cctgttctgc
    25861 agtgaaaagc tgtgggttgc aagtagtcta aaaactattg taataactct tataacgaga
    25921 agtcaaatga gtgggtaacg ccacaaaggg cttccactga tactccgaca tgcaacaagt
    25981 agtttatgca gaccaacgtt gtgtgaagct cggtgaagtc ggctgaagga taccgtaagg
    26041 taaaagaaat agtgaatacc acgaaagttc tttagaggta aagggtgtat ccgataagtc
    26101 gggtgtccaa aaaaacttaa tatggtgagt atgtacaaac gtactgacga actagcgaat
    26161 gtactgtccg aaaggcatag ctcctagaaa tgggagagac agtgagcaaa aaatcactca
    26221 ttgtgctaac ttgtggttga gtaaaagtcg ttgttatgaa agaccatgct gcgttacagg
    26281 cgtaggagaa gttagggggc aaaagctagc atctaagtta aggcatgtgg atagatataa
    26341 gggaacaggg gaagccatca acatggagag tgcatactcg atgaagtggt agtaagaaat
    26401 atgtgaatga caaataactt actttagtga tggtgaaagt agagtcacga ctgatgaagg
    26461 ttctgtaatg gaactggagg aacaggctcg attcgtctgt aaaagtcatg aaacccgttc
    26521 aaccatggga ttctcgtatg actaaaaagg tcacgagcca attggagcgt gataaaccat
    26581 gacaaaagaa acaagtccaa aagataggaa gttgcggcat gcagaatatt atggcatgac
    26641 ggaaatcttc gataagatat actccaaaag tttgaaggga gataatttca agaatctaat
    26701 gcagattatc atttctgata acaacattgt cctagcatat cgtaatatca agagaaatca
    26761 cggtagtgtg acaagaggag aagatggagt aacgattaaa gatattgaaa atctcaaccg
    26821 taatgaattc gtagaaattg ttaggaagcg attcgcacat tataaccctc ggaaagtaag
    26881 gcgtgtagaa attcctaaat ataatggtaa attaagaccg ttgggtattc catcaatatg
    26941 ggatagaatc gcgcagcaat gcatcttaca agtattagaa ccaatatgtg aagcaaaatt
    27001 caataatcac agctatggtt tccgcccaaa tagatcagca gaacacgcaa tagcagattg
    27061 tatggcccga atgaatcaaa gtcacatgga atatgtggta gatgtagata tcaaaggttt
    27121 cttcgacgaa gtcaatcata ctaaactcat gagacaaatc tggtctatgg gtatacgaga
    27181 caaacagctt ctcttaatca ttaggaaaat gttaagagca cccgtagtac ttccaaatgg
    27241 gaagttgata tacccaacaa aaggaacacc acaaggcgga atacttagcc cattacttgc
    27301 gaacatcaat ctcaacgaat ttgactggtg gatagcaaat cagtgggagg aaaaagatgc
    27361 caaagagcta aaacatcatt ttaggaaaaa tggagcttgg gataaaggta atatctatca
    27421 caagctcaga aagagtacaa ctctaaaaga aatataccta gtgagatacg cagatgattt
    27481 taaaattttc tgtagaaatt ataatgaagc gaaaaaagtt ttctatgcaa ctaaaatgtg
    27541 gttacaggag cgtctaaagt taccaatatc tgaagaaaag tcaaaaatca caaatctttc
    27601 aaaagaagaa agtgaatttt taggctttac gatgaaatta gtgaccaaag gtaacaaaaa
    27661 ggttgttaat acgcatatag gtacaaaagc acaaagcatg attaaagtga aactaaaaaa
    27721 acaaatcaaa cgaattcaaa aaagtcctaa tagcaaagat acctttatct atattggagt
    27781 ctataacagt atggttattg ggattcataa ttacttcaaa attgcgactc attgtagtgt
    27841 agcaatgcga aaaattgcaa gagaactcag accaagtttt gaaatacgac tacgagaaca
    27901 aggaatctca aaagaaggac aatacggaaa gtacgaagga tacaagcctt acctacggtc
    27961 aggacaaata cgatatataa gagggtttcc aatattacca gtagggtata ttcaacacag
    28021 acatccgatg tgtaaaaaaa gaaatattaa taaatacacc gaagaaggta gggaagccat
    28081 tcataagaag caacaaaccg tagaagaatg gaagatatcg tggttgtgta gcaatccagt
    28141 gcttagtaag agagcaacga ttgaatttgc ggacaatcga atttcaaaat tcatcgctca
    28201 aaagggaaga tgtgcagtaa caggtgagga gcttatctta tctgaaatgc attgtcatca
    28261 tattatccct tatcatgaaa gcaaaagtga tagctatgaa aacctagtga ttgttactga
    28321 ggaggttcat cgggtaatac acgctacaca aagtgaaacg attgaggaac ttctaaaata
    28381 tctaaaatta aatccaaaac aaaaagaaaa actcaatgag ttgagattaa aagttggtaa
    28441 cgaagaaata tcttaataaa tatttgtagg acgggaaaac agaatgtctt ttatagacga
    28501 gggaacgccg tatgcggtga aagtcgcacg tacggtgtgg actgggggaa aagacggaga
    28561 taatatcaaa ctcttaccta tcagtatcga ttaatccaga ttttattgaa acggtaaatt
    28621 atgtcacaaa ctttcaacga gaaatgcgta aagtagaagc aatgactgtt ttagctacac
    28681 aatccccaca agaaatggtg ccagaaaata tgtcttctga taacttctcg aaactgaaga
    28741 aagtctttga attttcttct acgaagatct ttatgcgtat ggatgaatct attttacgtc
    28801 atatagaagg attagtgggt aaatcaatga cgaactcaga attagaatcc attcctcaac
    28861 aaaatcaagg agacgctgtg atcaattttg gctcaaaaga aaccattcgt gttcatttta
    28921 gtccgaacaa acgtcaatta aaattgtttg atggaggaaa ataatggaaa accaaaatga
    28981 aagtttaatc aagcagtatg ttaagcgtcg tgcaaagcgg cgcttatttt tgtggttgtt
    29041 tggtacttct gcaggtttga ttactatttt gatcactgtg ttcgtgaccc tatttctaat
    29101 acttgcggcg ggctcgattg ataattcgga ttcagattca agcagtggtg gcgaagcttt
    29161 tacaggagaa tattcggaag gcttaccgat ttataaagaa atcaaaggaa gaggcccttt
    29221 ttctgatgag attgcgcaat atgccgtagg tgcagcagtg aaatataaat tattaccaag
    29281 tgtgatcctt tcgcaatacg gttacgaatc agcgtttggt acatcggcgt ccgcaagaaa
    29341 tgatctcaac tattttggga ttacttggtt tgatggttgt ctgtttccta aaggaactgc
    29401 acgaggaatt ggtggcattg aaggtggctg gtacatgaag tttcccaact caaaagctgc
    29461 attttcctat tacggcttta tggtagccac acaatcaaat tttaatgctt gtgtaggaaa
    29521 taaaagccct ggcgcaagct tattgatctt aggacgaggc ggttatgccg ctgcaggaat
    29581 tacggaagat agtccgtatt atacaggttg tatgtcaatt atcacttcta ataaattaac
    29641 cgaatatgat gaattcgcca tcaaacattg gggtgaaggt ggcaataaca acggcacgat
    29701 tactggtgaa tggacgaatc cattccctgg cagcagttta gataaaagtt ccttcagcgg
    29761 tggtcaatta tttggaacga atccaggcgg tgaattccgt ccgaacggct tccatgatgg
    29821 attagatttt ggttcagttg atcatccagg aagtgagatt catgcggttc atggcggaaa
    29881 agtcgtgtac gttggcaatc ctggtatttc aggtttgggc gcgtgtgtga tcgtgattaa
    29941 ttacgatggc ttgaatatgg tgtatcaaga atttgcaaat agtacaggaa attctcgagt
    30001 gaaagtaggc gatcaagtga aagttggtca agtaattggg atacgagata cagcacactt
    30061 gcatttagga tttactcgaa tggattggcg tcaagcacaa ggacatgcat tcattgatga
    30121 tggtacatgg atcgatccgt taccattttt gaatagcagt aaaaaataga tcaggagaaa
    30181 aaatatggaa aataaaacgt taaaaatcgt cttaacagct gcaattagcg ttagtttaat
    30241 cggcaatatc gcgttgtttt ggcagctgaa agaggaacaa gcgacaaata cctataaaat
    30301 tgaagaagtg gctaaaaacg cgtctgagaa gctcaaaaaa aattcctctg aggcacaggc
    30361 gaagatcgac aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg atcagctgaa
    30421 gcaacaagat ggcacaaaag caaaagagga agtaactgaa gcacaaagaa agacggcaga
    30481 agagttcggt caattggcca ttgataaatc attaagcaga aatgaattaa cggaaaaact
    30541 aaaagatgta gcaacccaag aagtgattga caaattaagt cctgcagacg atgatcacca
    30601 acatgatgat tacggaagtt actcctttac attaggtgac gtgcaaactt acgctcaaac
    30661 aagcagtgta aaagaggaac aaaattttgt cgtatttgtt gattatacaa tcggcaatcc
    30721 gcaattcaaa gatgtcaaac cacaaaaaat aaaaggtgga ttaactgtca ctgaaaaaca
    30781 agtagatggt caatggaaag tcatagactt tagttatttc actcgttagg aggaagaaaa
    30841 tgagtaagaa aaaaggagaa agacaacgtt tcaaaaaacg aaaaagttat cttcaatggc
    30901 tgggaatctt tatgattctc ggctttttga ttcttgctag tccgatttat acgaatcaga
    30961 aggtagatat accagatgag gtaagcacga acgcattttt aaacacgaag gaactgacga
    31021 tggtttcaaa agaaaaagga gcgaaccagc aattagtttt tgaatttgcg attgacgatg
    31081 aagatcaaag cttgttaaaa gaactggcta atcttaatta tcgcgtagaa gtaaagacag
    31141 ctaaagggga ttatcaagca ataaaagcca aagtgattaa ggtgtctgat gattattttg
    31201 cagtgaagct aacgcatgta ccagaagaat atattgctat gcgattaact attattcctg
    31261 aaaaaattga tccaaaggtg gatatgcaag aaccgcaaga tctgattttt tatattcacg
    31321 aagataaggt caaagatcac gtgaaagatg aagattatga acaacatgcg atcaattaca
    31381 aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa acaaattgaa tccttccaag
    31441 caaccattga tttgaatgaa gaattagtga agaagctaaa agatcaattg ccttatcaag
    31501 tcgcagatga tcaggaaaat actgaaaaca agattaatag ctaccagcaa gaaattgaaa
    31561 catcgaaaac gcaaatgaaa gatcaagaag agagcattca aaaacttcaa gaaaaaattg
    31621 atcgtatgac aaaagaaaag atttagaagg acaggtggag tgaaaaaatg aagcgaacaa
    31681 acttggctga caaagatact ttcgctgagc agaaagtatt gattaaagcc tatctcaaag
    31741 ttgtaaaagc taaagaaata tgtcgagaag tgaatttatc taacacaaca atatctgagt
    31801 tgaagaatgg tcgaaagaat ttagatactt taaaattcag atcatttgaa gctctatatg
    31861 tagctgctaa aaaatatttt acagctgata acaaataaaa aacaggagta ttttatgtga
    31921 aaaaagtata taaaaaatgc ttatgaatca gctatatcac cttaaccatt tgttaatgag
    31981 atgggagaaa acattaacca cgattctaac gcaaacatca agcacataaa acatttaaca
    32041 ctcctccttt ctggctgaag atttcaacca aacaggaaga cagcgtatca attatagcat
    32101 acactttttc ttaaattcat ttttcatatg acaaaatatt tccttctaat cgacaaagtt
    32161 agaaggattt tttattgtct aaatacacaa ataaccttaa tttttaaaga gcataaggaa
    32221 acgtgatatc actttcttac gaaaggtgct aggtaggctg tgcctaccac gtttctaaga
    32281 tgaaaatttg atatcaaatt ttagtaacta aggatagcaa aaggatagta aaggatagta
    32341 aaaggagggc ggtaggatag taaaaaggat atgctccttt ttactatcct aaagagaaat
    32401 ggatttaatt ataaggaacg tcaatgcaaa attcattaca gagattgaga gaagatgcgt
    32461 tgaattaagt aatcgcacag gaaataaatg gagtcgaaat gattacttaa agttactcat
    32521 tgaaaatgat tttgatcgtc ctttaatgga atataagcaa gaaaagttcg accagttatt
    32581 agagaagttt tcagaaattc aaagctataa cacaatgata ttagaagaat atgtcagcca
    32641 aaataatcgt attatagaaa tcttgattga gcaaaataag ggaagtgaac tttgatatgg
    32701 caaaggttat agacaaacat atttactttt atcaaacaca tatcgatata ttggatgaaa
    32761 ttatttcgac acatccagag gttaaaaact atagtgaagc tgttcgcttt ttatgtatga
    32821 attacgatcc agaaatggag aaaaaagtaa atgcaaatca agcaaaacta aatagtatga
    32881 gcaaagaaat atctactatg actgaaatta tagcagaatt tgcagatatt catttgaaag
    32941 atgcaggaat actaattggt acaaattcaa ctgtttatca agaagcaaaa gaacgtgtag
    33001 aagggaaaat aaaagcaaat caaacgaaga aatattctcc taagatcaag aaaccaatca
    33061 atgaatctgt tcatgccaga gtagatccat ttgatccaac acagttttta aaaagataga
    33121 aatgaaggag aataataatg actcaatcac ctgcagttat tctcacttgt caatttacct
    33181 tgcccaataa aaaatctttt tcaacgtaca ttgattatat gacaagagaa aaagcattgg
    33241 aagaaatgga acgacgtacg ccagaagaag aacaagaatt gcaacgaata aaaagtgtat
    33301 tgaatgattt ctatattccg gaaggagaaa ctttttcgga gagtaagacg gataaagaat
    33361 tcagtgaaac gtcaaatgaa gcgcaatcgt tgatggaaca tggaatcaat tttgacaagc
    33421 tagaggaaca agattttacc aaatatcttt cttatatgac tcgtcactat gcgttagaac
    33481 aaaagaaaga actgacgcat agtgaacaaa aagaatatca acggttgaat caagcaatga
    33541 aaaaatatca aacagaaccc cataaaaaag cttcaggaaa tcttcctgga gttttttcta
    33601 tatcttcaga cgaagtaaaa ggaagtgatc tgaaggagat caagcaaatc ttccaaaaag
    33661 ggcaacaaaa aggctcaatc atttatcaag atgttgtctc tcacgacaat tcgtatcttg
    33721 aaaaaatggg cttgtacgat cccaaaacag ataccttaga tgaagaaggt ttgaaagcag
    33781 ctggaagaaa aatgatggag acattattcg agaaagaaca attgaatgaa acaggttact
    33841 ggatggcaac gattcaccgt aacactaagc atatccatat tcattttgca gttgttgaaa
    33901 aagaaaatac gcgtaaaccg tatactgaga ttgaaaatgg tgtgagttat atcgtaccga
    33961 aggggaagag aagacaagca acattagacg caatgaaaac caattatgta catgagttag
    34021 aacgtttctc ttatgaaaag gatcgaatga ttggtatgga gaaacgaaat ttattgacac
    34081 gaaaaagcga tttgcgtaac accttaacaa aagtagtaaa agaaccagca cgttacgatc
    34141 gacatgccat gaacttatta actgaagtat ataaaacttt acccgacaaa cgttcagaat
    34201 ggaattatgg aaatgaaaat cgaaccaaac tttcaccata tacaagagaa aagctcgatg
    34261 aactaaccaa atatgtttta acaaaagaac ctgaatatca agaatatata cagatttcaa
    34321 aagcgttgaa aaaagaagca aaaagtcttt atggagaaag caaaagagag tcaaaagatg
    34381 cagagaaaaa cgctctcttt gatttgaaaa agcgtacagg aaatgcgatt ttaacagagc
    34441 tgaagagaca ggatagtcaa ataaaaccat taatagtaag tgtcaatcag atgcaatcta
    34501 tttacaatga acgatccttt cgaacactca aacagttgcc aacaaatgaa tacaagtata
    34561 gttaccaaga attcgtaaag cgtaggaaac aattccaaca aagagttgtc acgcagcgaa
    34621 gcttgcgtca attaaatcga acaattaatg cacatgaaga aaaataccac gccagtaaag
    34681 catatgaaga gcaacagcga agaatccaaa tggaacaaga aagacattag gaggaagaga
    34741 aatgaaacaa tatttagatc aatggaaagt catagaagga tcactaaaag aagaacgaat
    34801 cgaacaactt cctgattgtt tagaaaaaga gcatttgttt cagataagcg aaatgttgag
    34861 aaacgaacag tttgatccaa atcaattttt agttgttgag tacccagcta ctggtgtgta
    34921 ttgttgtaat catgtgaacg gtgaaaaata ttttattatt caagaatacg aaagtaagtt
    34981 agtgccttat tatactactt gggaaatgaa tgaagaggga atcaataatt tcccatgtga
    35041 aagtattaaa gagtctattt ctctcacgga atgttaaata aatagagacg taaaagagtc
    35101 ctgaacgtga ttgtccagga ctctttttca atgaattgtt ttccgccaat tcactgagtg
    35161 gtgcgattcg attttatt
//
LOCUS       DACCCM010000021        35019 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCCM010000021 DACCCM010000000
VERSION     DACCCM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.022"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IVK78_001053"
                     /gene_synonym="adhC"
     CDS             complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IVK78_001053"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP6374539.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IVK78_001054"
     CDS             complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IVK78_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP6374540.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IVK78_001055"
     CDS             complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IVK78_001055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP6374541.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4798..5496
                     /locus_tag="IVK78_001056"
     CDS             4798..5496
                     /locus_tag="IVK78_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6374542.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5664..8303)
                     /locus_tag="IVK78_001057"
     CDS             complement(5664..8303)
                     /locus_tag="IVK78_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6374543.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IVK78_001058"
     CDS             complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IVK78_001058"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP6374544.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10146..11321
                     /locus_tag="IVK78_001059"
     CDS             10146..11321
                     /locus_tag="IVK78_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6374545.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11325..11966
                     /locus_tag="IVK78_001060"
     CDS             11325..11966
                     /locus_tag="IVK78_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374546.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11963..12880
                     /locus_tag="IVK78_001061"
     CDS             11963..12880
                     /locus_tag="IVK78_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6374547.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12877..13548
                     /locus_tag="IVK78_001062"
     CDS             12877..13548
                     /locus_tag="IVK78_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374548.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14089..14577)
                     /locus_tag="IVK78_001063"
     CDS             complement(14089..14577)
                     /locus_tag="IVK78_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP6374549.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14778..15044)
                     /locus_tag="IVK78_001064"
     CDS             complement(14778..15044)
                     /locus_tag="IVK78_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6374550.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15044..15307)
                     /locus_tag="IVK78_001065"
     CDS             complement(15044..15307)
                     /locus_tag="IVK78_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6374551.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(15300..15572)
                     /locus_tag="IVK78_001066"
     CDS             complement(15300..15572)
                     /locus_tag="IVK78_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374552.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            15783..15968
                     /locus_tag="IVK78_001067"
     CDS             15783..15968
                     /locus_tag="IVK78_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6374553.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16110..17933)
                     /locus_tag="IVK78_001068"
     CDS             complement(16110..17933)
                     /locus_tag="IVK78_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP6374554.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18531..21236)
                     /locus_tag="IVK78_001069"
     CDS             complement(18531..21236)
                     /locus_tag="IVK78_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP6374555.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(21542..22489)
                     /locus_tag="IVK78_001070"
     CDS             complement(21542..22489)
                     /locus_tag="IVK78_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP6374556.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            22679..22948
                     /gene="rpsN"
                     /locus_tag="IVK78_001071"
     CDS             22679..22948
                     /gene="rpsN"
                     /locus_tag="IVK78_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6374557.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(23124..23486)
                     /locus_tag="IVK78_001072"
     CDS             complement(23124..23486)
                     /locus_tag="IVK78_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6374558.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(23497..24618)
                     /locus_tag="IVK78_001073"
     CDS             complement(23497..24618)
                     /locus_tag="IVK78_001073"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP6374559.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(24634..24984)
                     /locus_tag="IVK78_001074"
     CDS             complement(24634..24984)
                     /locus_tag="IVK78_001074"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP6374560.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(25114..26625)
                     /locus_tag="IVK78_001075"
     CDS             complement(25114..26625)
                     /locus_tag="IVK78_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6374561.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(26947..28299)
                     /locus_tag="IVK78_001076"
     CDS             complement(26947..28299)
                     /locus_tag="IVK78_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP6374562.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(28452..29528)
                     /locus_tag="IVK78_001077"
     CDS             complement(28452..29528)
                     /locus_tag="IVK78_001077"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP6374563.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            29724..31469
                     /locus_tag="IVK78_001078"
     CDS             29724..31469
                     /locus_tag="IVK78_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP6374564.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(31749..32186)
                     /locus_tag="IVK78_001079"
     CDS             complement(31749..32186)
                     /locus_tag="IVK78_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP6374565.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(32170..33543)
                     /locus_tag="IVK78_001080"
     CDS             complement(32170..33543)
                     /locus_tag="IVK78_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374566.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(33865..34353)
                     /locus_tag="IVK78_001081"
     CDS             complement(33865..34353)
                     /locus_tag="IVK78_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6374567.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(34375..34977)
                     /locus_tag="IVK78_001082"
     CDS             complement(34375..34977)
                     /locus_tag="IVK78_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6374568.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9919 a         7476 c         6466 g        11158 t
ORIGIN      
        1 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
       61 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
      121 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
      181 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
      241 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
      301 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
      361 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
      421 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
      481 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
      541 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
      601 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
      661 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
      721 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
      781 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
      841 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
      901 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
      961 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
     1021 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
     1081 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
     1141 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
     1201 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
     1261 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
     1321 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
     1381 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
     1441 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
     1501 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
     1561 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
     1621 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
     1681 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
     1741 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
     1801 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
     1861 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
     1921 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
     1981 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
     2041 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
     2101 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
     2161 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
     2221 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
     2281 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
     2341 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
     2401 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
     2461 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
     2521 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
     2581 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
     2641 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
     2701 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta
     2761 tttttaaata taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat
     2821 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
     2881 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
     2941 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
     3001 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
     3061 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
     3121 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
     3181 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
     3241 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
     3301 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
     3361 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
     3421 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
     3481 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
     3541 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
     3601 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
     3661 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
     3721 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
     3781 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
     3841 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
     3901 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
     3961 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
     4021 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
     4081 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
     4141 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
     4201 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
     4261 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
     4321 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
     4381 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
     4441 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
     4501 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
     4561 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
     4621 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
     4681 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
     4741 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
     4801 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
     4861 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
     4921 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
     4981 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
     5041 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
     5101 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
     5161 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
     5221 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
     5281 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
     5341 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
     5401 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
     5461 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
     5521 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
     5581 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
     5641 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
     5701 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
     5761 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
     5821 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
     5881 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
     5941 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
     6001 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
     6061 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
     6121 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
     6181 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
     6241 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
     6301 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
     6361 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
     6421 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
     6481 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
     6541 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
     6601 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
     6661 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
     6721 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
     6781 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
     6841 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
     6901 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
     6961 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
     7021 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
     7081 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
     7141 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
     7201 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
     7261 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
     7321 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
     7381 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
     7441 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
     7501 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
     7561 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
     7621 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
     7681 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
     7741 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
     7801 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
     7861 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
     7921 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
     7981 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
     8041 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
     8101 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
     8161 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
     8221 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
     8281 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
     8341 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
     8401 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
     8461 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
     8521 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
     8581 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
     8641 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
     8701 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
     8761 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
     8821 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
     8881 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
     8941 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
     9001 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
     9061 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
     9121 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
     9181 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
     9241 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
     9301 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
     9361 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
     9421 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
     9481 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
     9541 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
     9601 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
     9661 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
     9721 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
     9781 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
     9841 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
     9901 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
     9961 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
    10021 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
    10081 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
    10141 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
    10201 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
    10261 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
    10321 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
    10381 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
    10441 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
    10501 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
    10561 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
    10621 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
    10681 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
    10741 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
    10801 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
    10861 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
    10921 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
    10981 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
    11041 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
    11101 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
    11161 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
    11221 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
    11281 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
    11341 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
    11401 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
    11461 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
    11521 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
    11581 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
    11641 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
    11701 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
    11761 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
    11821 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
    11881 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
    11941 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
    12001 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
    12061 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
    12121 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
    12181 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
    12241 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
    12301 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt
    12361 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
    12421 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
    12481 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
    12541 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
    12601 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
    12661 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
    12721 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
    12781 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    12841 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    12901 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    12961 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    13021 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    13081 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    13141 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    13201 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    13261 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    13321 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    13381 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    13441 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    13501 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    13561 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    13621 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    13681 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    13741 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    13801 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    13861 aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    13921 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    13981 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    14041 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    14101 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    14161 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    14221 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    14281 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    14341 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    14401 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    14461 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    14521 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    14581 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    14641 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    14701 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    14761 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    14821 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    14881 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    14941 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    15001 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    15061 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    15121 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    15181 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    15241 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    15301 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    15361 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    15421 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    15481 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    15541 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    15601 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    15661 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    15721 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    15781 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    15841 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    15901 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    15961 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    16021 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    16081 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    16141 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    16201 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    16261 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    16321 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    16381 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    16441 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    16501 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    16561 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    16621 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    16681 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    16741 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    16801 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    16861 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    16921 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    16981 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    17041 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    17101 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    17161 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    17221 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    17281 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    17341 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    17401 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    17461 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    17521 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    17581 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    17641 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    17701 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    17761 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    17821 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    17881 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    17941 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    18001 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    18061 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    18121 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    18181 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    18241 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    18301 gcttgcatct gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg
    18361 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    18421 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    18481 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    18541 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    18601 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    18661 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    18721 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    18781 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    18841 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    18901 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    18961 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    19021 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    19081 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    19141 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    19201 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    19261 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    19321 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    19381 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    19441 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    19501 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    19561 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    19621 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    19681 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    19741 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    19801 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    19861 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    19921 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    19981 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    20041 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    20101 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    20161 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    20221 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    20281 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    20341 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    20401 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    20461 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    20521 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    20581 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    20641 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    20701 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    20761 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    20821 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    20881 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    20941 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    21001 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    21061 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    21121 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    21181 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    21241 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    21301 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    21361 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    21421 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    21481 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    21541 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    21601 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    21661 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    21721 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    21781 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    21841 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    21901 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    21961 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    22021 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    22081 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    22141 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    22201 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    22261 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    22321 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    22381 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    22441 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    22501 ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt
    22561 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    22621 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    22681 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    22741 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    22801 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    22861 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    22921 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    22981 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
    23041 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
    23101 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
    23161 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
    23221 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
    23281 tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt
    23341 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
    23401 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
    23461 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
    23521 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
    23581 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
    23641 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
    23701 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
    23761 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
    23821 gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc
    23881 tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc
    23941 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
    24001 gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc
    24061 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
    24121 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
    24181 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
    24241 cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc
    24301 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
    24361 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
    24421 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
    24481 atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
    24541 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
    24601 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
    24661 agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt
    24721 cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
    24781 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
    24841 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
    24901 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
    24961 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
    25021 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
    25081 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
    25141 tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct
    25201 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
    25261 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
    25321 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
    25381 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt
    25441 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
    25501 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
    25561 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
    25621 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
    25681 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
    25741 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
    25801 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
    25861 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac
    25921 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
    25981 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
    26041 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
    26101 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
    26161 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
    26221 gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
    26281 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
    26341 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc
    26401 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
    26461 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
    26521 tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac
    26581 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
    26641 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
    26701 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
    26761 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
    26821 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
    26881 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
    26941 agatttttat ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct
    27001 gccttttagc ataatactat ctgttgctgc aagattttgt ttgatagcat caatcaattt
    27061 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
    27121 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
    27181 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
    27241 taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc
    27301 catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt
    27361 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
    27421 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
    27481 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
    27541 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
    27601 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
    27661 tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
    27721 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
    27781 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt
    27841 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
    27901 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
    27961 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta
    28021 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt
    28081 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
    28141 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
    28201 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
    28261 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
    28321 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
    28381 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc
    28441 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
    28501 cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
    28561 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
    28621 tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
    28681 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga
    28741 atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct
    28801 tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg
    28861 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
    28921 gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc
    28981 atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
    29041 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
    29101 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
    29161 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
    29221 tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc
    29281 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
    29341 tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct
    29401 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
    29461 gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta
    29521 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
    29581 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga
    29641 acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
    29701 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa
    29761 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
    29821 aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc
    29881 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
    29941 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
    30001 tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg
    30061 ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc
    30121 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
    30181 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
    30241 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
    30301 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
    30361 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg
    30421 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
    30481 caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg
    30541 atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta
    30601 gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
    30661 cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
    30721 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
    30781 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
    30841 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
    30901 ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata
    30961 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
    31021 tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac
    31081 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
    31141 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
    31201 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
    31261 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
    31321 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
    31381 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
    31441 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    31501 agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    31561 ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca
    31621 cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc
    31681 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    31741 ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc
    31801 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    31861 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    31921 actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    31981 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    32041 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    32101 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    32161 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    32221 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    32281 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    32341 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    32401 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt
    32461 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    32521 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    32581 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    32641 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    32701 gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    32761 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    32821 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    32881 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    32941 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    33001 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    33061 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    33121 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    33181 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    33241 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    33301 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    33361 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    33421 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    33481 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    33541 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    33601 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat
    33661 aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca
    33721 aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg
    33781 tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa
    33841 tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat
    33901 ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt
    33961 ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt
    34021 tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg
    34081 tactttattt agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc
    34141 agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag
    34201 ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg
    34261 accaatgatc ggcacaaaag cttccggcca tacttcttgt atgactgcca tcagctcatc
    34321 cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt tacattataa
    34381 atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca
    34441 tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc
    34501 tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt
    34561 tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga
    34621 taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc
    34681 cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat
    34741 gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa
    34801 cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt
    34861 gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag
    34921 accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta
    34981 tattccccac atcttttttc atttattaaa actgcctcc
//
LOCUS       DACCCM010000022        34522 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCCM010000022 DACCCM010000000
VERSION     DACCCM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.023"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(133..306)
                     /locus_tag="IVK78_001083"
     CDS             complement(133..306)
                     /locus_tag="IVK78_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6374569.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IVK78_001084"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IVK78_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IVK78_001085"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IVK78_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP6374570.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IVK78_001086"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IVK78_001086"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP6374571.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IVK78_001087"
     CDS             5704..6489
                     /locus_tag="IVK78_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6374572.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IVK78_001088"
     CDS             6502..7353
                     /locus_tag="IVK78_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP6374573.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IVK78_001089"
     CDS             complement(7477..7929)
                     /locus_tag="IVK78_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6374574.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IVK78_001090"
     CDS             complement(8394..8762)
                     /locus_tag="IVK78_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP6374575.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IVK78_001091"
     CDS             8912..9394
                     /locus_tag="IVK78_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP6374576.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IVK78_001092"
     CDS             9431..10441
                     /locus_tag="IVK78_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP6374577.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IVK78_001093"
     CDS             complement(10556..11269)
                     /locus_tag="IVK78_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374578.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IVK78_001094"
     CDS             complement(11459..12049)
                     /locus_tag="IVK78_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP6374579.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IVK78_001095"
     CDS             12533..12760
                     /locus_tag="IVK78_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP6374580.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IVK78_001096"
     CDS             12776..13942
                     /locus_tag="IVK78_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP6374581.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IVK78_001097"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IVK78_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP6374582.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IVK78_001098"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IVK78_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP6374583.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IVK78_001099"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IVK78_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP6374584.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVK78_001100"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVK78_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP6374585.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IVK78_001101"
     CDS             complement(19155..19820)
                     /locus_tag="IVK78_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP6374586.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IVK78_001102"
     CDS             19796..19969
                     /locus_tag="IVK78_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6374587.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IVK78_001103"
     CDS             complement(20014..20751)
                     /locus_tag="IVK78_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6374588.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IVK78_001104"
     CDS             complement(20753..22255)
                     /locus_tag="IVK78_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6374589.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IVK78_001105"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IVK78_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP6374590.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IVK78_001106"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IVK78_001106"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP6374591.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IVK78_001107"
     CDS             27597..28862
                     /locus_tag="IVK78_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP6374592.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IVK78_001108"
     CDS             28866..29597
                     /locus_tag="IVK78_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP6374593.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IVK78_001109"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IVK78_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP6374594.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IVK78_001110"
     CDS             30613..31611
                     /locus_tag="IVK78_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP6374595.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IVK78_001111"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IVK78_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP6374596.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="IVK78_001112"
     CDS             33123..33773
                     /locus_tag="IVK78_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374597.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34522
                     /locus_tag="IVK78_001113"
     CDS             33960..>34522
                     /locus_tag="IVK78_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP6374598.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11453 a         5832 c         7325 g         9912 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 tgaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata ga
//
LOCUS       DACCCM010000023        33610 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCCM010000023 DACCCM010000000
VERSION     DACCCM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.024"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(462..887)
                     /locus_tag="IVK78_001114"
     CDS             complement(462..887)
                     /locus_tag="IVK78_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374599.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1261..2232
                     /locus_tag="IVK78_001115"
     CDS             1261..2232
                     /locus_tag="IVK78_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6374600.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2264..3661
                     /locus_tag="IVK78_001116"
     CDS             2264..3661
                     /locus_tag="IVK78_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6374601.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRWVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3820..4443
                     /locus_tag="IVK78_001117"
     CDS             3820..4443
                     /locus_tag="IVK78_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374602.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(4669..5193)
                     /locus_tag="IVK78_001118"
     CDS             complement(4669..5193)
                     /locus_tag="IVK78_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6374603.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5186..5659)
                     /gene="tsaE"
                     /locus_tag="IVK78_001119"
     CDS             complement(5186..5659)
                     /gene="tsaE"
                     /locus_tag="IVK78_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP6374604.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5782..6762)
                     /gene="pta"
                     /locus_tag="IVK78_001120"
     CDS             complement(5782..6762)
                     /gene="pta"
                     /locus_tag="IVK78_001120"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP6374605.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6915..7595)
                     /locus_tag="IVK78_001121"
     CDS             complement(6915..7595)
                     /locus_tag="IVK78_001121"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6374606.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7721..8590
                     /locus_tag="IVK78_001122"
     CDS             7721..8590
                     /locus_tag="IVK78_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6374607.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8616..9131
                     /locus_tag="IVK78_001123"
     CDS             8616..9131
                     /locus_tag="IVK78_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374608.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9178..9741)
                     /locus_tag="IVK78_001124"
     CDS             complement(9178..9741)
                     /locus_tag="IVK78_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6374609.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9796..9975)
                     /locus_tag="IVK78_001125"
     CDS             complement(9796..9975)
                     /locus_tag="IVK78_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374610.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10052..10561)
                     /locus_tag="IVK78_001126"
     CDS             complement(10052..10561)
                     /locus_tag="IVK78_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374611.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10657..11037)
                     /locus_tag="IVK78_001127"
     CDS             complement(10657..11037)
                     /locus_tag="IVK78_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6374612.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11367..12116
                     /locus_tag="IVK78_001128"
     CDS             11367..12116
                     /locus_tag="IVK78_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP6374613.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12300..13577)
                     /locus_tag="IVK78_001129"
     CDS             complement(12300..13577)
                     /locus_tag="IVK78_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374614.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13656..14300)
                     /gene="pcp"
                     /locus_tag="IVK78_001130"
     CDS             complement(13656..14300)
                     /gene="pcp"
                     /locus_tag="IVK78_001130"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6374615.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14499..15443)
                     /locus_tag="IVK78_001131"
     CDS             complement(14499..15443)
                     /locus_tag="IVK78_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6374616.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15445..16131)
                     /locus_tag="IVK78_001132"
     CDS             complement(15445..16131)
                     /locus_tag="IVK78_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6374617.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16605..16925
                     /locus_tag="IVK78_001133"
     CDS             16605..16925
                     /locus_tag="IVK78_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6374618.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17079..17984
                     /locus_tag="IVK78_001134"
     CDS             17079..17984
                     /locus_tag="IVK78_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374619.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            17981..18754
                     /locus_tag="IVK78_001135"
     CDS             17981..18754
                     /locus_tag="IVK78_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6374620.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(18935..20359)
                     /locus_tag="IVK78_001136"
     CDS             complement(18935..20359)
                     /locus_tag="IVK78_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6374621.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20570..21016
                     /gene="nrdI"
                     /locus_tag="IVK78_001137"
     CDS             20570..21016
                     /gene="nrdI"
                     /locus_tag="IVK78_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6374622.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21081..23063)
                     /locus_tag="IVK78_001138"
     CDS             complement(21081..23063)
                     /locus_tag="IVK78_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6374623.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23077..23973)
                     /gene="murQ"
                     /locus_tag="IVK78_001139"
     CDS             complement(23077..23973)
                     /gene="murQ"
                     /locus_tag="IVK78_001139"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6374624.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(23976..25037)
                     /locus_tag="IVK78_001140"
     CDS             complement(23976..25037)
                     /locus_tag="IVK78_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6374625.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25248..25775)
                     /locus_tag="IVK78_001141"
     CDS             complement(25248..25775)
                     /locus_tag="IVK78_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6374626.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(25842..27344)
                     /locus_tag="IVK78_001142"
     CDS             complement(25842..27344)
                     /locus_tag="IVK78_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6374627.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(27602..28723)
                     /locus_tag="IVK78_001143"
     CDS             complement(27602..28723)
                     /locus_tag="IVK78_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6374628.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            28849..29790
                     /locus_tag="IVK78_001144"
     CDS             28849..29790
                     /locus_tag="IVK78_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6374629.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(29940..30836)
                     /locus_tag="IVK78_001145"
     CDS             complement(29940..30836)
                     /locus_tag="IVK78_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6374630.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            31105..32445
                     /locus_tag="IVK78_001146"
     CDS             31105..32445
                     /locus_tag="IVK78_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6374631.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            32663..33106
                     /locus_tag="IVK78_001147"
     CDS             32663..33106
                     /locus_tag="IVK78_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6374632.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT         9959 a         6748 c         6150 g        10753 t
ORIGIN      
        1 aaaaattact acgtgatgta gaataagcct ttctatcgtt tggttatttt atattcaaat
       61 atttgaaata aataaatctt tactctttga tttgtaaact gaaatgatcc actgtttgtt
      121 ttactgatga agcttgatca aaggaagaag ctgagtttga agttcataaa aatcagccat
      181 agtgagtttt gcttggattt gtttatcttc ttctactttt tacataattt tgaacaggat
      241 aagattcaaa tgttttaaat gaatctttga ctagtttgtg acagttgtcg catatgctca
      301 agcttccttg gtcattaaat tctgtatgga tatttttgtt gttattacat gtacaataat
      361 caggactatc gatcatccat ttcgtctcct ttacaatcat cttgcttctt tactatagca
      421 gaaaagaagg ggagaaacaa aagaagatcc aacagacggc actatagact gtagatgttg
      481 ccatcctcat ccttcaaacg gtaattaccg atttttgtaa atatgactgt tgtttctttt
      541 ttattttctt ttcctgaaat aatatattcg tcatctttgg tagtgatctt gatatctgtc
      601 agcactgttt tttcttcaga tgattcagaa gaatcagttc cttgtgtctg atacgtgcgt
      661 gtccattcca tttttttatc tgtaaatgtc aactgtcccg cggataaatc catatcttta
      721 cgtactttta gcgtatatac actgttggtg gtatctgctt tttccgcttt taagacagaa
      781 gaatcggttg tttcgtccga ggattggtca gagccgccaa acaaatcaca gccagtcaat
      841 gtcaaaagtc ccgcagacaa aataaatagt acgattaatt tcttcaaaat acttccccca
      901 tttgatagga taaagaaatt ataccattgg aatgtaaaac ttcaaacgga tggaagagaa
      961 aaacgcacaa aacacccaca aatagagtaa attttagaat aaaattttaa aaaggcaaaa
     1021 aatccgccac tttcagaaag cgttttaaaa aagtgttgca atcttttaaa aaaatgctat
     1081 attaaaaatg aaccggttca aatacttgtt taagtataat taatagtgag aggaagttgc
     1141 tctactgaag actttagtta tatggatatt gatgacaagc aacactatgc aaagaatgcg
     1201 gtcatcataa tcgaacaaca agaaagagaa atcaaatgta actggcagga gtgagaatgt
     1261 atggcatcga tccgtgatgt ggctaagatg gctggtgttt cagtgggaac agtttcccgt
     1321 tacttgaatg gtcagcaact gaaggaaaag aacatgaaaa aaatagcaga agcgatttca
     1381 gcgttggact ataaagaaaa catcattgct aaagggttga agaataatcg aagcttctct
     1441 attggacttt tgataaacgg gatttccagc cgttttgggt cggaagttgt ctcagggatc
     1501 gaaagagtag ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca
     1561 gaaaaaattg aacaaaaaat tgaatacctg atgaagcatg tgatcgatgg gctgattgtc
     1621 ttcttatccg aagaagagtg gtctggattt gaaatgctca caaaaatgag tattccagtt
     1681 atcgcattga attgtccaga aggaccagca ggtattgata caatattagt gaatgatcga
     1741 gaaagtgtag caaaagtgat cgctcatcat ggtgaacaag gacataaaaa aatcggattt
     1801 attgctgcca ctcaaacaga ctacgtagca agagaacgtt tagcaggagc caaagaagcg
     1861 gtccaaaatg atccatcgat ccaattagag gtctttacag gagactattc tagaaaaagc
     1921 ggctattacg gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat
     1981 tacaatatgt cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag
     2041 gatattgcgt tcagtcacta tgattattta gataaaaacg atctgagtat ccttccgaaa
     2101 atcacgatca cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttaaaa
     2161 cgaatcaaaa aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat
     2221 ggaatcaact aaaacgaact aaaaaagaaa aagaggtgct ttgatgaaaa aatatcaatt
     2281 tccagcaaat ttttggtggg gttctgcagc aagtggccca caaacagaag gaaggatttc
     2341 aggcgatgga aaaggagaga acatttttga ttattggtac aaaaaagaac cgcaaaagtt
     2401 ctttgatcaa gtaggacctg aaaaaacctc gcaagtttat acgaagtatc aagaggatgt
     2461 acagttgatg aaacagactg ggcataattc atttcgaaca tctatccaat ggagccgttt
     2521 gatacctgat gaatctggaa caatcaacga agaagccgtt cgtttctatc atcgttattt
     2581 tgatgagttg attgaaaacg gtatcgaacc ctttgtgaat ctgtatcatt tcgatatgcc
     2641 gatgtatctg caagaaaaag gcggatggtt gaaccgagaa acggttgaag cctatgaaaa
     2701 atatgccaaa agttgttttg aattgtttgg ttcgaaagta aaaaaatggt ttactcataa
     2761 tgaaccaatc gttccagtcg aagcaggtta tttatatcga tggcattatc cagaagagtc
     2821 agatatgaaa aaagcgatcc aagttggcta tcatgaagcg ttggcttcag cgttagccat
     2881 caaatcttac catgaaatgg agcaaggtgg tgttattggt atcatcttga atctgacacc
     2941 aagctatcca agagacgaat caaaccctga agatgttaaa gcagcgcaaa tagcagatgc
     3001 gttcttcaat cgttcattcc ttgatccagc tgtaaaaggc gagtttccaa gtgaattgat
     3061 tgagatagta aaagaacttg atctcatgcc tgtgatccat gcagaggatt tgacggttat
     3121 caaagaaaat acagtagatc ttttaggtat caattattac cagccacgct gggtaaaagc
     3181 aaaagaaaca ccaatcgata ctaaaaacgg tccaatgcct gaggattatt tcgacaatta
     3241 tgaaatgcct ggtcgtaaaa tgaatcctta tcgcggatgg gagatctatg aacaaggaat
     3301 ctatgatatt ttgatcaatg tacgagatca ttatggaaat atcgactgct ttatttctga
     3361 aaacggtatg ggtgtagaaa acgaagcacg atttatcaaa gaagatggca tgatccacga
     3421 tgattatcgg atcgaatttg tccaatctca tctgaaatat gtccatcaag ccattcaaga
     3481 aggtgccaat tgtttaggct atcatatgtg gacatgtatg gataactggt cgtggaccaa
     3541 tgcatataag aatcgatatg gctttatttc agtagactta gcacaagatg gaaaaagaac
     3601 aatcaaaaaa tcgggtcatt ggttcaaaga cgtatcagat aaaaacggat ttgaggctta
     3661 agaaagaata gttgagagca aatgcgtgaa attctccatt cttaccattt tgtgaaaaag
     3721 aagttcaatt atttacgatc tttttcaact tttttgtctt tctatttcat tttttgtata
     3781 taatagaata gtcaaaatta aataggagtg gaggaaagca tgaggaaaaa aagaccgttt
     3841 cttctctttt ggttattcgg ttttttctta ctgcttattt tatccgcagc attttcagga
     3901 aaaatgacag cagatcttgc tcagaaagaa acagaaaaaa tcgtagcaag caaagaatca
     3961 gtagataatt tcaaaaaaga attctcaact tattggtggg cggagtataa aataactgcc
     4021 aattacgaaa aaggcgaatt ggacatttat ttgccaaaag gaaaagaaga aaatcaattt
     4081 attaatatcg atgacgtact tagtgcgatt tctctcagac aatatcaatt taaagacaag
     4141 catttgaccg ttttgctttt gactcatgat ggcgacaaag tagcccgttt gaaaaatgga
     4201 aaagtgatca ccgcttattc tgaaaaagta gacaaaaaag tggtcgccga tcgtttagcg
     4261 gaatggaaaa agacaaatga acaagtcaag aaaacaactg tctcttctca aaaaaacgag
     4321 tcgacaggaa caagtccttc agattctggc agtggacaaa cgataacggg aagtgaagaa
     4381 aatcaagcct caattattac gaatgagtca tcacaaggac aatatcaatc ctatggacaa
     4441 tgaaaaacag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggcaa
     4501 aatagttcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
     4561 cgtttattca aaaaaaatgg actgcgacac gacttttgtc acagtccctt cgtgtgtttt
     4621 tctgccttct caaaaggatg acagtttctc actcttttcc tatttcttct attcaatcaa
     4681 ataactcatt ttatatacat ctaaaaattc acctagatct gttcttgccc ctctaggcat
     4741 gaccgcttcg atttggaagc ccatttttcg gtaaaggtgt acggctcttt cattacgaac
     4801 ttgaacgtca agctccaatc gaaaaagtac acccatttcc ttcgcccaac tgatgatttc
     4861 ttctagcata agagtgccta gtcccattcc ccaatattcc tgcagaatac tgataccgac
     4921 ttctcccacg tgagacaaac gaaattgact gtctgccttg acagatgctg ttccaataat
     4981 cgtcccttca tagattgcaa gcagcaataa attattattg ctttcgtata tataatccaa
     5041 ctcttcttct aaggtttctg gtggtaacag gagttctgct tcatccaaaa ctaaaaattc
     5101 tgtctcctta ttaacttgtc ccatcatcaa aagaatgtct tttgcatctc ctggcactgc
     5161 ttcccgcaag atgatcccaa cttcttcact catgattttc agcccatttt tctagaattc
     5221 gctgtttgaa ggtttcactt tgctccccgt atgagattag tttcacatac cgcttttcta
     5281 aatctgtatc acttttttct aaaatcagtt ccaaataatc ctccggcagt gcttcttcta
     5341 atagatttcc ccattcaaca acggaaagcc catcaccttc aaaatattca tctaacccta
     5401 aatctgctcc gctagctgct acccgataaa tatccatatg atacaacgga atccgacctt
     5461 gactatattc tctaataatc gtgtatgtcg gacttttgat catttgcgtt atccctaatc
     5521 cttgggcaat tcctttcgtc aacgttgttt tacccgcacc tagatcacca gtcaatatca
     5581 gattgtcacc tggcattgcg gaatgaccaa ttgcctctgc gaattgttct gtcattgtta
     5641 atcctgatag ttcgatcatt tttcatcttc tcccgctctt ctttcttttt gccagtatat
     5701 cgcgtaatga agttttttta aagaaaaaat gaactagtca agaacagcag ctgctgtttt
     5761 tggctagttc gcgtgtattt gttaatcaat caatgtttgt gctgctgtga taatcgataa
     5821 tttatataca tcttcttcat ttgctccacg agacaaatca gaaaccggtt tgttcaagcc
     5881 ttgtagaatt ggtccgattg cttcaaagtt accgaaacgc tgagcaattt tatagccgat
     5941 atttcctgac tgcaattctg gaaatacaaa gactgttgct tgaccagcta cttctgaatc
     6001 tggcgctttc agttgtgcaa ctgaaggaac ataagaagca tcaaattgca attcgccgtc
     6061 gatcatgtat tgtggcgcta gttttttagc gattttagtt gcttcaacta ctttgtctac
     6121 ttctggcgat ttcgctgagc cttttgtcga gaagctcatc attgctactt ttggttcgat
     6181 atcgaacaat tctgctgttt ttgcagaatc tacggcgatt tcagccaatt cttgcgcagt
     6241 tggattcacg ttgatcgcac aatccgaaaa gacatatttt tcttgatctc ttccacgtac
     6301 catgatcatc gctccgcttg tccgactgat gccaggcttt gttttgatga tctgaagtgc
     6361 tgggcgaaca gtgtcgccag tcgaatggat cgctccagag accattccgt ctgcaatccc
     6421 catatacgta agcatcgtac caaagtaatt cacatctttt aaaatctttt ctgcttgttc
     6481 tttggatgcc tttccatttc tacgttcaac aaaagtatcc accatttcat cccacttatc
     6541 gtaattatca gggctgatga tcgtaaagct agatgttttg attcctctgg catgcgctgc
     6601 atttcttatt tcttcttcgt tccctactaa aatgggttcc acaagttctt ctgctttcaa
     6661 ccgggcagct gcgcctaaaa tacgagcatc agtagcttcc gggaaaacga ttttgatccc
     6721 acggcgaaca attttaaatc ttaaactatc aaacaattcc acaagatgac ctccagacgt
     6781 ttttatatgt attatcatac actattccag aaaaaaagaa cagtttattt tatctgtatc
     6841 agtcaaaaat gctggaaaaa ttaagttttt cacatacaaa gcgaactgca ctataacagg
     6901 aaagcgctgt tttattacac tgtttcaggc agttgccaat caatcggttc ttctcctaac
     6961 gcaaggagcg catcattcgt tttcgagaag ggtttggaac caaaaaagcc acgacttgca
     7021 gataacggac tgggatgtgg agatgttatg atgatatggc gactcttatc gatcattttg
     7081 attttttctt gtgccggttt tccccacaag ataaacacga ttggtttttc ccgttcattt
     7141 aatttttcaa tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg agaatacgct
     7201 tgcccttccc gaacggtaag aacggtgttg agtaaaagga ctccctgttt agcccatgac
     7261 actagatttc catgttgaac aggtgcgatt ccaagatcat cataaagctc tttatagata
     7321 tttcttaacg atggcgggat ctttacgcca ggttgcacag aaaaagataa gccatgagct
     7381 tgattttctc catgataagg atcttgtcct aaaatcacca ctttaacttc ttcataagga
     7441 gtcaattcca atgcttcata gatatgatac atatctggat ggatcttttg ggacgcatat
     7501 tcttttttta aaaattctct tagatgcaga taataatctt ttgaaaattc tgaggacaaa
     7561 acttcttgcc aactgttatg aatgatttct ttcaaaaact ccacctccta ataaaaagaa
     7621 taactcacat tttcttcaat cacaagggac aatcttcacc ctcagcactt gattaatatg
     7681 gtaaactagg aacaagaagc agaaaggaac gataatcatt atgatcaaat taattgcatc
     7741 agatatggac ggcacattat tagatgccca tatgagtgtt tctcctgaaa atgccgaggc
     7801 gatacgtttt gcaaatgatg ccggtgttga gttcatggtc gctactggtc gaaactatca
     7861 agaagctcgc gcagctttag atgaggtagg aatcgattgt gcgatgatca ctttgaacgg
     7921 ggcacaggta tttgataaag aaggaaattc attgtttacc gtgtcgattc caaataatca
     7981 agcaatctct gttttagata ttctagatgc taacggtatt tactatgaag tagcaaccaa
     8041 cgatggtctt tattctgaaa atcaagcgaa acgaattgaa agttttgctt ccatggtggc
     8101 tacccattta cctcacctta cgtataaaat ggcgattgcc atggcctcag ctaagctgga
     8161 gttattgcac atcacatacg tggacagtat tcgtgctatt ttggaagaaa agaatctgga
     8221 agttttaaaa attatttgtt ttcatacaga agggccagct gtattaggtc cagttggaaa
     8281 gcagatttct cttttaggcg atctagccgt tacttcctct ggtcaaaaca atatcgaagt
     8341 gaaccataaa aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg agcgtgggat
     8401 ttctttagaa gaagtcatga cgattggcga taacttcaat gatgtcagca tgctgcaagt
     8461 agcaggagtc agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt atgccaaata
     8521 tacaacggac acaaaccttg aatctggcgt aggaaaagcc atattgcgcg cgattgcaga
     8581 agacttgtag ttttatctag aaaagaggga tatagatgtc agaatttttc attcaggaaa
     8641 agcagcttgg acgtgcaact cggacaattg tcaaaaacga aaaaggacat tccctgtttt
     8701 tgatggttgg tcgctgggga acaaagggcg atgcattgtc tctttatgcc atgaatggcg
     8761 atctcgttgc tcacatcaag cagataagcc tgacattcgg tacgcgtttt gagctgtata
     8821 aagagttcga aaaggtggga accatgcaga aaatattcaa ttggcccggt gacttttatt
     8881 atattcgtag acttcactgg acagtccaag gtgatatcta taatcatcaa tatcaaatcc
     8941 atcatttcaa tcagcaaatc atgaaaatgg ataaagcaac acttttttca ggagattact
     9001 atgtattgga tgtctcaaat gatgaagatg ctccagtttg tatttgtatt gctgcggttt
     9061 tagattactg gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct taccgactta
     9121 acttaagtta aaaagatgta cctgaaatgc ggaaatacgc atttcaggta catcttttta
     9181 gatattcttt tgcatatgat cataaagatg accactgatc atccgctgac cgacaacttt
     9241 gaagtcatgg cgttcgtata attttttagc ctttggattt ttttcatcca cacttagacc
     9301 gatgacggag cgattagctt tcttcgccaa tttaggtaat gcttctaata atcgcgaacc
     9361 gatgccttgt cctctaaaat cttctgatac actgatagag tccaaatacc attcgttggg
     9421 aaacgtttct ttatcaatga acaaacgtgt agacgcatct agctgatatt tctctaagat
     9481 aggtaccagt ggctgatcga tgattgattc ttcttcatct gtataaccgt aagcaactcc
     9541 tgcaacttta ccgtctattt cttctacgat acctcgtgaa taactgtaac gataatttgg
     9601 atcagtgatt gcttcttcta aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa
     9661 tggcagttcc atatctttta gaatcactaa gatcaatggg gcgatttctg gagcatcttc
     9721 ttttctcgca aaacgaatca tagtttcctc tccttttctc cattctaaca tgcagaaaaa
     9781 gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg
     9841 agtaacgatt cttgccgctt cagttcggca ataagggcag acgactggca tttctttatc
     9901 attatgttcg actacattat tgacttcttc gtatgcaaat ttttttccgc aattagggca
     9961 tttcattttt tccatgttat ctcaacttcc tttcatcttt actctcttag cttactctta
    10021 tattgtaaaa aagagaagca atatccttct tctatgcaga tatccatacc tgacggtcct
    10081 gttctccatc tattaatcgg actttataat aaggacgttc atctttgact agaagtgtcc
    10141 actctttcac ttttgctttt ttcccgtagt agctgactgc ttccttcatc gctgcttgcg
    10201 gttgtttgaa ctcttccgtt ttttctatat caaaaagatg ttctgcggcc ttttccgtgt
    10261 catttcgtac ggttgttttc ccagtggatg gattgactgc tatgacttgt ccttcacaaa
    10321 aaaactggta ttcatatgtt gcattcgtag gattctccaa atctttttca actgcaaaat
    10381 gaatagcaga tatgttatgt tcttttgttt cgttttttac gattgctgct gcttttttca
    10441 atgagatccg atattcctgg tcactaatat catccatgtg aaaagcggca ttgagcagtc
    10501 ccgtcatacc ggtaatccct attacgatga gggcagcaac accaaacatc atttttttca
    10561 ttcttcattc tcctgtttct tttttttaaa gaatatcaag aaatgaacga atgaaaaatc
    10621 gaatgaaacg aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt ctaatggcaa
    10681 tccgtctgga tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt ctctaatcgg
    10741 ttctgattcg atccctaagg actgcaattc tgcaattgtt tcttcaatat cgtctacttt
    10801 gaaagcaaga tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg gcgcattagc
    10861 aattccaaaa atctccaatt cagcacttcc tagtttcaga tctaatttat agtcatctcg
    10921 ttctttgcgg taattttctc tgattacttc aaatcctaat ttattaacat aaaaatcacg
    10981 tgatttctga taatctgaaa caatgatggc tatatgatgg atcgttgtaa ggttcatatg
    11041 agcactccta ttcttcgttt aagctcggat ctatattaat ctccagttcc attgtaaatg
    11101 tgtcagattg aaaaaaatag ctgtttttcc tttttataag aaaaaaggca aaaaaaacag
    11161 aaagtaggtc ctccaagaca ctcttcctgt tcccaatata ataaatgaaa gtaagaaatt
    11221 atattttcaa gaaaacaaac agaaaattat cataacacaa attatttata attgaaagac
    11281 tagttttttt tcaagaaaag catacaatag attgtgttag taaatacaga acacactaca
    11341 tttagtgtct taaggagtcc tcatacatga agtcatattt tcagttttta gctcatcagc
    11401 taaaaggctg gccgcaacaa aattattatc ttttcttttt cagtcttggc tgccaggtca
    11461 tgactcttgt caatagtccc atcacttggc tgtccgttat tacatttatc ggtaccacgc
    11521 ttggtgtatt gtgtatttta tccatcaatg cggcaaaatc agtcaacggt attttaggta
    11581 ttttgtctgc gatttgtttt attatcgttg ggttttcagc aaagaactat ttaagtatcg
    11641 gtgaacagat tgcttatatg atcacattgg atatccccgt attgttaagt aaagaatgga
    11701 atcataatat ggcttcaaaa atccgaaaat tcactggaaa aagctggttt atcgcaatca
    11761 tcagtacttt ccttgtctat cttgtttcag gattcctgat tggtcgttat acagatgatc
    11821 ctcggccgtg ggtagatgcc attgcttttt ctattagctt gactgctggt atcatcagtt
    11881 tcttgcgtta caacaaccaa tattactggt ggttagcttc agggcttgct caaatggtcc
    11941 tttggtatat ttcatttacg catggatctg caagtttggc gatgttcgtg aacagctctg
    12001 tttacttgct gaatgatgtc cttgcattta ccgtttcacc ttggtataac caaaaagaac
    12061 gtgaacgtat gcaaaaaatg gaagaagaat actacaacgc acaacaacac gcataaaaaa
    12121 actgagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    12181 gcggaatcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    12241 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctattttt
    12301 tatagtaatt ccttcagata ctttcttgcc tcatcattga aacgcagtga ttcttctaac
    12361 ttctgataat attccaacgc ttgtttctga gcttcagtaa aacgataatg ttttcctttc
    12421 catttgtcga ttgtctcttg cacatttcca gattggatat ctttcactac tagttgccat
    12481 ttttcttttg agactgtacg gtctttctta tcagcagatt gtatagcttc tcgttcttta
    12541 cgcaatactt ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa gaacccttct
    12601 gtaaaaccat agtgacaata ttgcttccaa ataaatagat agatttttgg ttggatgaat
    12661 cgttggtttt tcgcaaaata ttccatctga gcagtgactt ctggtgttcg ttgacgtttt
    12721 gtctttctca acacggaaat aaatgtttca aaataagcat taggatgtac tggcacttct
    12781 tcatatacga tggattgcca atctttacgt aaaaatgcgc gatagttgat caagaattct
    12841 tctacatcct ttactaattc ttctgcaaag tcgtcaaata gttctttttt tatctgtcgc
    12901 ttttttgact gcccattgat gtgttcactt attttcttga tttcattttc caatgcttga
    12961 taatccgcta tgtaaatcgg catttctact tggcgaaatt ctgcgagccg ctgagctaat
    13021 tttatatccg gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac aaaacagtat
    13081 ttttgccata gtccttcagg aaaatcgata ttcttctgct taagccataa taattcgtct
    13141 aaggccagat caacttttgt cattccctga tggatcgaag ctgccactgt cggagagaca
    13201 tgcaggtgat cggcaaagtg gttgatgcct agttttagga tctttccggt ttttggcgat
    13261 ttaacgatgt attgcacttt taattctcta ccacactcac aatacaattt ttgctggctt
    13321 cgttttgttc gaaaaaacgg attgatcttg aagtcagaaa acacccaatc tgttgcagct
    13381 aaataatgtt cttccataaa acgagagctg attagatatc ggcggtgctt atcgactagt
    13441 tcccgctgtt ttttcgttaa ttttgcatag atctcccaac gttgactgct tgtgtattgt
    13501 ttagaaattt tgatgggata gcttttgctg atttcttttg gaaaccattc ttcaaagacg
    13561 gattgccatt cttccacggc tgtttctttc ctttctaatt acaaaaagct ggctactaac
    13621 ataacgttag caaccagcca gtgtttcttg ctaaattagt gagttgttcc ggcatttccg
    13681 gaaatatctt ctttattttc tactgctgct tcaactgcat aagtcaaaga agaagcaatg
    13741 tcttctaaag acatagaagg cgtacccact ggacgatcga tcacttgatc gggtgagaat
    13801 ggcacgtgga tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa atgaagcaaa
    13861 cgatacatga tatcattgca gacaaatgtt cctgctgtat aagagatgta cgctggtaga
    13921 ccatgatctt gtacattttt tgtcattgct ttgattggca atgaggtaaa gtaagcggct
    13981 ggaccgtttt cttctaaagc cgtatcgaat ggctgatttc cttcattatc agggattctt
    14041 gcttctgcta gattgattgc gacacgttcg aacgagactg ctgaccgccc acctgcttgt
    14101 ccgacacaga tcacgacatc tggttggtgc tgttggatgg cttctttgac tacttgactg
    14161 cttttttcga atacggtcgg tacttccact ttgatgattt cagcaccgct gatttcatct
    14221 ggcatttttt ttactgcttc ataggctgga ttcactttgt ccccaccaaa tggatcaaaa
    14281 cctgttacta gtactttcat ctgttgttcc tccgtccgat tgatttaaca tatggttatc
    14341 ttttatcata ccattttcct tgaacagcgt aaataaagga aacaatagtt taaataacat
    14401 aacagatcaa acttaatata ttcttataaa aaaatagtgt tccactacaa tgttagaaga
    14461 ggttgcgaca attattctgt cacaacccct ctcttctatt atttaaatag aatcatataa
    14521 gcaatttgga aaactagcat aaataacgca ataaacaact ggtttttgat cactccgtat
    14581 ttgtctttca tctccagcat cgctacaggc acgatattga agttagcagc catcggtgtc
    14641 agcagggtcc cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc tggattcgct
    14701 ccatgactaa atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat ggccgcaaat
    14761 gcattcccca tgattaccgt aaatatcacc atgccaagag cgtaaatgat gatgccgatg
    14821 ttcacatttc cttctggaac gatcgtacct actgcattgg aaatcactgt cccaactcca
    14881 gcgcttgtaa ataccgcacc tagagaagct aaaagcattg gcaacatgct taacggccct
    14941 actgtgccca gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt atctcgagaa
    15001 aagaacatca agattagcat ggctgcaaaa acaccgacgc ccacgccgac taaagcacct
    15061 aaattggtaa atagagcaaa caagatcgca aagacaccga tactcaaagc tggaataaaa
    15121 attttcattc ctagtttgtc tgacattttt tccatatatt cttttgatgg aagccggctt
    15181 tctcctttgc tgactttctt agcaattgct ggtatggtca tcgcaaagat caagccaccg
    15241 ttcacgattg ccggaagcca tcttcctcct gcaataacta cccctaagac cgtccagaaa
    15301 aaggctgacc cataacgatg aggattggtt ttatctgtta aatttcttac tccagtgtaa
    15361 atcaatacga gtcccatcac gatataaagg atctccagta atttatcgcc taataaaata
    15421 tcaggatttg taaaaaagtc catcttattg cccctttcct ggataatatt tcttcgctaa
    15481 tttcttttct ttaagataga agtagacact tgccacaact aaagccgtga tagccactgg
    15541 aacttccact aaagcaagct gtcccaatgt tacgtcatat cctagatctt tcagtgttga
    15601 ttggactagc aacgcccctg ctcctccgat gaatagtact tggccaaaga accagcagac
    15661 attttccata gctgaagcca taccttttag ttcttcttca tgcttaggat ggacttctaa
    15721 cccttttgat tcgatggtcc ccaatgccat cggtaaaatg attggacgaa caaatccagc
    15781 aactccacca aaactcacgt taaaagccgc aaagatcatt cgaaagatcc cataaatatc
    15841 tacaatcata ccagcagata ctttcttgaa tcgcttgatc aaggaagccg ctgattcttt
    15901 cagaccattt ctttcaagcg ttcctgttgc caacataatg atgataaaaa tcgccatccc
    15961 gcgattgttt acaaaacttg tacctaaaag ttctaacatt ccttgaatgc caagaccgcc
    16021 agtcaaagct gtaacaacta aagcaaccat tacgatcaat atcgagtcta atttaaatgc
    16081 aaacccgaga ataacaatca agacgcctaa aaggctaaga taattttcca tatattttcc
    16141 tccataaaat gacaatattt ttttaattat aaagcaaaac acataaaaaa tatagttgtt
    16201 ttctcacgaa atagacacta tttttaaata tttttcgtta tttttttatc acaaaaatta
    16261 tcttacttat tgtaaggtaa agcttttttt aatttattgc ccgataatat atagaaataa
    16321 ggtctagtta ctgttttttt taaatgatta ataaataaca acttctttct atgaaattgc
    16381 aaagcattca tcttcttttc cgctaacgat atatccgtca gtgcttggta cactttacta
    16441 aaaagtatga gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacaa
    16501 tctgccaccg ggtaggataa agaggatgat cattccatta ggtctttgaa gggatgattt
    16561 tcctcttttt gtgtattttt ttatagttaa ggaggaatgt aagtatggca tggatggaat
    16621 taatcatagc aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt
    16681 tttctaaaat caattattct ttttacacag tgattggcat gattgctagt ttttactttt
    16741 tgtcaaaggc aatcaaaagt ttgccgatga gtctctctta tcctatctgg acagggattg
    16801 gcgcagttgg ttcggtcata attggtgtgg tcttgtttca tgataagctg aatctttcca
    16861 cttggttctt tgtggggatg ctactcatca gtatcattgg catcaagatt actagcggcc
    16921 actagacact tggaataagt actgatcaga aaaaataagc ctttttattt aatattcaat
    16981 ttttttatga aaaaataaga aataagtcca atgacctagc gacttctcca ctcatcaggt
    17041 gtatgcttgt actatctata gaaaaggagt gaatattttt gaaaagtctt tcggtccaag
    17101 aattatccaa gaactttggt cacaaacaag ccctcagcga tgtcagtttt agcttatccc
    17161 ctggcaaaat cgttggatta gtaggtccta atggcgctgg aaaaacaacg atcatgaaag
    17221 ctattttagg actgatccat tactcagatg gaacaatctg tattggtgaa aatgagattt
    17281 ctccctctag tcataaaggg ttggaacaag taggagcatt gatcgaatac ccgggtatct
    17341 atccattctt aacaggctat gatcatctga agttattctc tgaaaccaac gatgtatctg
    17401 ctatcgacac tatcgtgaat cagctcaaga tggaaaagta cattcacaaa aaagcgaaat
    17461 cctactcgtt gggaatgaaa caaaaattgg gaattgctct tgccttactg aaccatccgt
    17521 ctatcgttat tttagatgag ccgatgaacg gtttggatcc acaagcaaca agagatgtgc
    17581 gaaaaatcat taccgacttg gctactcagg gcacctcttt cttgatctcc agtcacttac
    17641 tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga aagatcatca
    17701 aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa
    17761 caaatgatct agctgctcga acgatcttga cagaagctgg atatactttg gaagatcctg
    17821 ataaaatcaa gcttttaaaa gaaaacgaag aattattagg aaccgtgatc cagctattgt
    17881 ctgcacaaaa tatccatgtg acagacgtcc agcatctgac gaatgatttg gaatcttctc
    17941 tattggaaat tctggattca gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca
    18001 agaattattt aaattgatca agaaaaagtc aactgctgtt ttatctgttc tattagttgt
    18061 tttgctcata ggaacagcac ttctagccaa aaaatacacg acgatcattg atccagttga
    18121 aatgactgct cagcttttta gtgccacatc ttggattgtc tttatcatga ttgcagctgc
    18181 aagcacgatc atttcgatgg aagcacaata tggtacattg aaaaatctcc tttatcgaaa
    18241 gtattctcga ggagaaatat tagtaagcaa atggatcaca ttagttattt attctgttta
    18301 tttatacctt ttagctatta ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat
    18361 ttccttcacg gagaaggttt ctactggtca aacactgatc caaagcttgt tcacttatac
    18421 tttgggtagt tatatcggtt tatggctgat tttaagttta gtgttgatga ttgcttgctt
    18481 tatcaatagt tccggcgcat ccatctcagc aggaatcgtc ttttattttg cttcgtcaat
    18541 catatctggt atccttattg cactcatcca aaaatgggag tggatcaaat ggaatccgat
    18601 cagtatgttg aatctgcaaa accaaattgg aaacgaagag atcatgaagc aattgactca
    18661 tctttctact aatcaaatgc tattcggtaa tttagcttac atcgttttgt tcttagcatt
    18721 aggatatctc gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa
    18781 agagacgttc agccttattg ccgattggct gtctcttaaa caccgtttat tctgaaaaag
    18841 gagctgtgac aacatttgtg tcacagctcc tttcctagta tgtttttgaa taaacgacag
    18901 gctcacaacc tctctttata cttcttttat acttctatac gtgaagcatc ccagtggcaa
    18961 ataaaaccat tgcaatcact ccgaccatcg tcacacctat aagtaaaatc ttatctaagt
    19021 ggatatgttg tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg
    19081 gcaagaaaag aacaagaaca agcattactt cattaaaggc tacgactagc atagcccata
    19141 cttgaaaggc acttgctaaa agccctacta aaaattggac ccattctttc cgctgataag
    19201 aaagtttcat ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa
    19261 gtgtgtaagc aaagttataa gcactgtcag tgaataataa tgaaagtaaa aagacattcg
    19321 tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc
    19381 atgctttagg caaaagtccg tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg
    19441 tccatgagag ccaaacacca actaaagaaa tgatcaaacc gccgttgatc acgactgctc
    19501 cccatttacc aaccatcatt tcaaacacat agcccatagc aggctgcgcc ttgatcgttt
    19561 gcagctgatt catcgtcatt gtcccatatg gaagcatgga aaccaagacg taaataataa
    19621 tcaagccgaa tatccctaga atcgttgctt tcccaacatc tgattgtttt tttgctcgtg
    19681 aacctacgac agaggctcct tcaataccaa caaataccca aagcatcacc atgatgcaac
    19741 tcaatacttg actggaaaac gggatcggct gtccattcgc attcaatata atgtcgctag
    19801 aaaagttatc aatgaattgt tggatcgaaa acgtgctgat agctactaca ataaataaaa
    19861 ataatggcac taatttacag aatgtgacga tagcattgac gaaactagca ttttcgactc
    19921 cccgattcac tagccatgca tatccccata aaaagactgt actcatcacg atcgatgcca
    19981 gattttgccc accttcaaat actgggaaga aatatcctaa tgcgctcatc aacaatgttg
    20041 caaaagctac gttgcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag
    20101 cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt
    20161 ttttattgat attattgatc gacaaggaga gcatcaacat tgctgttcct acgagtaccc
    20221 aactgaaaat agctgggccg ccagccgatc cggagatgtc cgtcataata ccaaatacgc
    20281 cacctccaat cgctgaacca attactaagg cagcaagtgg aattaaacca attccttttt
    20341 tctgttgctc tgtatccatg taagcatcct ccaaaataaa accgctttta tcaaaaagta
    20401 taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac
    20461 cggctttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa
    20521 aaacttaaaa aagatgctat actaaaagga aagaattgga ggatgatcca tgaacatttt
    20581 atatatatcg atttcaggaa atacgcgtgc ctttgccaaa catctagcag aatatgctga
    20641 aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa
    20701 tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg
    20761 cggaaatggt ttagataacg gtgatacaga aatcttgacc gaaacgatgc gtgaatatct
    20821 ggaacatgaa gacaatcata aactttgtct tggtgtagtc ggtagcggaa ataaaaactt
    20881 caataatcag tattgtctaa cagccaaaca atatgcacaa acatttggct ttcctttctt
    20941 ggcagattat gaattacggg gaacaccatc tgatgtgaca cgaatttatc agattttagc
    21001 agaaaacaat aaataataaa aaagaagaag ctgaaaaata gatttcagtt tcttctttcg
    21061 tttctattgt ttctatcata ttaagctttg acatccatca caggtgcttt tgcttttgct
    21121 ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac caagacgatc
    21181 atggatgttt ctttgcctgc tgctttgatc gcttctaagt cagcttctgc tacaagcgta
    21241 tcaggtgtca cttgctgccc ttctgttact ttcacatcaa atggcgtacc gcctaagtca
    21301 actgtgttga ttcccatatg aagcaagatt tctaaaccat ttggcgttgt gatcccaata
    21361 gcgtgtttcg ttgggaagac agagctgact accccagtaa ctggagaata gatatgtccg
    21421 ttttctggtt ctacaaagta gccttcaccc atcatctttt gtgcaaaaac tggatcactt
    21481 gctttatcta aagaaacaac tgttccgtca gcaactgatg agaggatagt cacactttca
    21541 gctgaagttt gaggtactac tttttcaggc acttcattta ctgtggctgt ttcagtcgct
    21601 aattcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc aacgaagcca
    21661 ccagcataag ctgccaataa tccaaggatg tagcctaacc atttaccgtc agagatcaat
    21721 gggatcaaag cggctcctga aggtccaata gcgatagccc cgacatttcc gatcattccg
    21781 atcactgccc cgccgatacc tccaccgata caagcagtaa taaatgggcg tcctagaggt
    21841 aatgttactc cgtaaatcaa tggttcgccg attcctagga tcccgactgg taaagctcct
    21901 ttgatcattt ctgttaattc tttatctttt ttgcagcgaa tccataatgc caatgctgca
    21961 cccacttgtc ctgcaccagc catcgcaaga attggaagca atagcgtacg accagtttca
    22021 tcaatcattt gcacatggat tggagtcaaa atttgatgca gtccgaacat aaccatcggc
    22081 aagaataatg tgcctagaac gaagccggca aatgctccac caacgccaag tacccagttg
    22141 atcccaccga ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca
    22201 atcagaccga tggcaatcaa cgaaagagtt ggtgtaacga tgatatctac cgcatcaggt
    22261 actactttat gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa gatgacacct
    22321 aggatcccac cttgtccagc tgcaagagct tcaccagtaa ataagttttt cagtggtgct
    22381 tccggattca tccctgtcag catcacaacg gccccgataa ctccaccaag agttggtgtt
    22441 gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctaggtagct aaacatcccg
    22501 tttttcaaga tgttcatcac atcgatatat tgttgccatg ttgctgcatc aagtgttccg
    22561 gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt accgacaaat
    22621 gcaggaatca ttggcacaaa gatattagaa atgtctttta ataccgattt gattttagat
    22681 ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt tacacggtct
    22741 tttccagatg ctgatgaatt tgctgtagct gtacttacag aagaaagttt ttctcctaat
    22801 ttcacgcctg ccaaatcaac catttgctgt gctactttgt tcactttccc aggtccgatg
    22861 atgacttgaa gctgttcatc ttctactaca ccaagcactc ctggaatcgc ttttaattca
    22921 gagacattta caagatcatc attgatgatc ttcatacgca cacgagtcat gcagtggaca
    22981 atgctttcta cattgcccat gccgcctgcc cctttataaa tttgttttgc cagttcttcc
    23041 aaagtcagtt cgttttttgc catggtcttt tccccctcat aaggtccctt ttacaaatcc
    23101 gtttgcttca gctaattttg cttgagcttc ctctttatca ctatttgtca aaatcatgac
    23161 gatagccagc ttaacatttt gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc
    23221 agcttctgtc gcttgcatga tgattcgttt tgagcgttcg attagttttt catttgttgg
    23281 tttgacatcg accatcaagt tattgaatac tttacctata ccaatcatac tgatagtaga
    23341 aatcatattc aagataagtt tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa
    23401 aaattctgga cctgcatcga cttcgattgg gaaatccgcg tgtttactga tttcggcttg
    23461 tttattgcaa gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat ctaatccacc
    23521 gatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat tttttgtcaa
    23581 attcagcttg attagatctt cttgccctag ctcataagaa tcttcggccc cttcgacagc
    23641 tacagtcatt gcttcttctc caccagcaat tagacctatg accatttccg gttctacacc
    23701 gaatgtcggt acgcattcag cagcatctaa cacacctagt cttccacttg ttccggctcc
    23761 catataaatc agacgtccac cttttttaaa ggactcaatc gtgttagcaa tcactggttc
    23821 gatacttgtc agttcttttt caaccgcagc ggccactttc tgatcttctt tgttcattag
    23881 ttgaacggct tgagaaatac tcatctcatc taatccaaaa gtttcttggt ttctgcgttc
    23941 cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt tcttataaat
    24001 tgatagttca taccagcttt gatttggttg atcaatggta gctcttcttt gataacttta
    24061 gctactacat tcactttttc atccgctggc aaatcatatt tggttaattg gatctcaccc
    24121 atatagcgca aatacttttc gttatctaaa gtaatagacc ctttcatacg cgtagctgta
    24181 tttcttgctg ggataggcgg aatctctcga aaacgtgcat ctacgcttcg tatgacatct
    24241 cttgcatcgt cttgccgatt cgtatgcttc cctaatatat attcgtaaaa ttcttcatct
    24301 acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa ttgctcctga
    24361 acgttttctg acagacctgc atctccgatg taaacaatat ccgtgttact tgctaataga
    24421 tctagagcgc ctgcaagagg atggatccct cgatgttctt ccaaagttgg caatccttga
    24481 tatagtgggc ctctcaactc tgtatctcct ggtacaaaac cttggacaag gagaccttga
    24541 attttgagcc attgattttt tttctgatac cattctttgt ccagccctgt ttcgggacgt
    24601 ggataataat tatgccaagc ttcaatctgt gagaaatcag cttctgcttc tttcaattca
    24661 tcgatatctt taggcgtgat cgtgctagca ttcaaactga ttttcaattg atgtgaccag
    24721 tcggcaatct gctgattgga aatatgataa tccattctta aacctgttac accaattgct
    24781 ttcaaaggcg ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata
    24841 tcgaccatca attctagttt attctttttc gcttcgcttc ctagttcgat caatcgcttt
    24901 tgatatgccg aaacatcatc ttctggtata tgcatggaag tgaaaatacc gacaaagcca
    24961 ttggatgcca tttggcggat atactgtttc ttctcttctg acaaagaatc gttcataaat
    25021 attgaaaagc caaacatatt atccctcttt ttctgtatcc gtcttcattt ctatgactag
    25081 agtatatatc taaagaaaca aaatttcaat acttttatta aaataattaa atttcatttc
    25141 taaaggttga gtttatatta tcaaaagaat tgtcataacc taaaaaaggc actgctaagc
    25201 aaaagcagtg ccttgaaata ttttatttaa ataacacaaa aacataatta ctcataagaa
    25261 accaattctg tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa
    25321 taagcattga tcactcttat caaggcatta tggcaaacta acagatagac attttcttcg
    25381 tcttcgatgc attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc
    25441 ccatttggga atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt
    25501 ggcagtccat cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc
    25561 aagctgagac gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg
    25621 atgattttcg tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc
    25681 atctgtcctt tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc
    25741 caagttgttt ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaag
    25801 ttccccttaa ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa
    25861 ataatctcag tagatacttt atccaggaag gtttgatcat gttccactag taacatggca
    25921 ggtttcacgt tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc
    25981 aatggttcat cccatgtata tagttcagct ggctgtgata aagatttagc caattccact
    26041 tttttccgtt ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc
    26101 ccaagctttc ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat
    26161 tccgccaacg ttcctcgatt gtcttcataa ttttggcttg cataactaat gctcagatgt
    26221 tttggctggt atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata
    26281 atggatgact ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt
    26341 gtaaaatgaa ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt
    26401 aaaagtcgct tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata
    26461 tcttttaata gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg
    26521 gatcgtttca tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat
    26581 tcagtatcga tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat
    26641 tcggcttttt cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt
    26701 tgagccattt caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct
    26761 tgataaagtt ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata
    26821 aatccccggt catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct
    26881 acttgttttc taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga
    26941 gtgtcatcca caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg
    27001 ctgaattctc tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt
    27061 tcccatatct caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct
    27121 tttgtctgtt gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt
    27181 agtttgtttt gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt
    27241 cctagtttcc attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg
    27301 ctgtcgtagc cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta
    27361 aaaaaagacc agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac
    27421 aaaaagaccc atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt
    27481 gtctccacaa ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac
    27541 ggatccctca ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca
    27601 actacttcgg gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt
    27661 ctaatgtttg cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta
    27721 agacaattgg gagaaaatca gaaagtcctt ctagctcttg gtctctactt ccgcaaatag
    27781 cgattattgg ttttcgatat ttttttgcaa gcactgctac accgtatgga acttttccgt
    27841 ggatcgtttg ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct
    27901 ttttttccaa tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca
    27961 acaaaaaaag ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat
    28021 ccacctccca gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag
    28081 ctagcgtcgt atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac
    28141 ctgtatatgg attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt
    28201 ttcttcgatc aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt
    28261 ctaaagaagc acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc
    28321 ctaaagtgac aataatttgt tctacttctt gaagcagtgc atctttaata agttctccta
    28381 aggcataact ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc
    28441 ccaatgcttg tgcagattcg atgactgcgg tgttttttcc ttgccaagtt gtcaataaat
    28501 agctgatttt tcgctgacgg aataagagat ccatcccatc aatggtacgc catgttcccc
    28561 tgagcgcgta atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga
    28621 tttttcttcc tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag
    28681 aagcacttcc tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc
    28741 ttcttaagta agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat
    28801 ttttctatat actaaaaggg aactcattga ataaaggaga gaaaaaacat gaaaatcgca
    28861 atagctgggg ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat
    28921 gaggtcctct tgatcgatcg ttggcctgct cacattgaag ccattcgtac aaatgggttg
    28981 attgctgact ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa
    29041 atcatcgaaa gcaacgaaca cgtggacttg atcgtagcat taacaaaagc caatcaatta
    29101 gatgatatgt tttgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta
    29161 ctaaatggat taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta
    29221 tttgggatca cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt
    29281 gatggagaaa tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt
    29341 gtagaagtct ttcaagaagc aaatttaaat ccgatctata gccataacgt tcgttattcc
    29401 atttggcgaa aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt
    29461 aatattgctg aactaggcgc gcaaaaagcg gcagagagca tggttcgaac aatcgtatct
    29521 gaatttgctt ctatcgcagc aaaagaagga atcatactag atcaagaaga agtgtaccaa
    29581 catattgctt ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa
    29641 gacctgatca aaaaccatcg tttgactgaa atcgactaca tcaatggtgc catttggcga
    29701 aaaggacaaa aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat
    29761 gcaaaagaag gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca
    29821 gcagctggtt ctcttttttt aagcccaata aaagatcaaa aagaaaacac aactctcatg
    29881 atgccataag aattgtgctt gtttgtttca gtacataatc tcttcaaaat gtaggttttt
    29941 tatttgtttt caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta
    30001 gtgataaatc gttttgccca attttctgca tcataaaatt ccaatagctc tgataggatt
    30061 ggcttaaatg gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa
    30121 aaaatatttt caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt
    30181 ttcttttgaa aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc
    30241 aacagttcca gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagtaaga
    30301 tatccaggtt gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt
    30361 tttataaatt cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca
    30421 aaaaatagat ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa
    30481 ctattatgtt tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg
    30541 attgccggtt catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc
    30601 caaaaaaata acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata
    30661 tcaaatccat aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat
    30721 ttaatgaatt ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa
    30781 ggcaaaaaat aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt
    30841 tccgcctcac tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca
    30901 gtaaaacgtt tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata
    30961 aatcattctt ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa
    31021 tttgttatca ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt
    31081 gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc
    31141 agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt
    31201 ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg
    31261 attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat
    31321 cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat
    31381 tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc
    31441 tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt
    31501 ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg
    31561 gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt
    31621 cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg
    31681 tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac
    31741 ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt
    31801 cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt
    31861 ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg
    31921 atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc
    31981 cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc
    32041 cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta
    32101 ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat
    32161 tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg
    32221 atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg
    32281 gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg
    32341 ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc
    32401 agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt
    32461 ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt
    32521 gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa
    32581 accctctgtg caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat
    32641 gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt
    32701 ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt
    32761 cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat
    32821 gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc
    32881 tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga
    32941 cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg
    33001 agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga
    33061 agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca
    33121 aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc
    33181 tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    33241 caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc
    33301 ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag
    33361 acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc
    33421 tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa
    33481 aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg
    33541 gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt
    33601 tttatgtttt
//
LOCUS       DACCCM010000024        30349 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCCM010000024 DACCCM010000000
VERSION     DACCCM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.025"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            171..515
                     /locus_tag="IVK78_001148"
     CDS             171..515
                     /locus_tag="IVK78_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP6374633.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IVK78_001149"
                     /pseudo
     CDS             516..935
                     /locus_tag="IVK78_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="IVK78_001150"
     CDS             complement(1027..1377)
                     /locus_tag="IVK78_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374634.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IVK78_001151"
     CDS             complement(1343..2674)
                     /locus_tag="IVK78_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP6374635.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IVK78_001152"
     CDS             2930..3496
                     /locus_tag="IVK78_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6374636.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IVK78_001153"
     tRNA            3641..3728
                     /locus_tag="IVK78_001153"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IVK78_001154"
     CDS             4062..5036
                     /locus_tag="IVK78_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6374637.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IVK78_001155"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IVK78_001155"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP6374638.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNETIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IVK78_001156"
     CDS             complement(7451..7897)
                     /locus_tag="IVK78_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6374639.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IVK78_001157"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IVK78_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6374640.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IVK78_001158"
     CDS             8362..8880
                     /locus_tag="IVK78_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6374641.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IVK78_001159"
     CDS             complement(8957..9913)
                     /locus_tag="IVK78_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6374642.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IVK78_001160"
     CDS             10079..10885
                     /locus_tag="IVK78_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374643.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IVK78_001161"
     CDS             10882..11871
                     /locus_tag="IVK78_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6374644.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IVK78_001162"
     CDS             11868..12872
                     /locus_tag="IVK78_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6374645.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IVK78_001163"
     CDS             13004..13546
                     /locus_tag="IVK78_001163"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP6374646.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IVK78_001164"
     CDS             13566..14264
                     /locus_tag="IVK78_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP6374647.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IVK78_001165"
     CDS             14285..14497
                     /locus_tag="IVK78_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP6374648.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IVK78_001166"
     CDS             14497..15459
                     /locus_tag="IVK78_001166"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP6374649.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IVK78_001167"
     CDS             15477..15872
                     /locus_tag="IVK78_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6374650.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IVK78_001168"
     CDS             16035..16400
                     /locus_tag="IVK78_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP6374651.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IVK78_001169"
     CDS             16397..18208
                     /locus_tag="IVK78_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP6374652.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IVK78_001170"
     CDS             complement(18270..18437)
                     /locus_tag="IVK78_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP6374653.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IVK78_001171"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IVK78_001171"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP6374654.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IVK78_001172"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IVK78_001172"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP6374655.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IVK78_001173"
     CDS             21194..22174
                     /locus_tag="IVK78_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6374656.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22263..23087
                     /gene="tsaB"
                     /locus_tag="IVK78_001174"
     CDS             22263..23087
                     /gene="tsaB"
                     /locus_tag="IVK78_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP6374657.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23071..23613
                     /gene="rimI"
                     /locus_tag="IVK78_001175"
     CDS             23071..23613
                     /gene="rimI"
                     /locus_tag="IVK78_001175"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6374658.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23620..24084
                     /gene="rimI"
                     /locus_tag="IVK78_001176"
     CDS             23620..24084
                     /gene="rimI"
                     /locus_tag="IVK78_001176"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6374659.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24081..25097
                     /gene="tsaD"
                     /locus_tag="IVK78_001177"
     CDS             24081..25097
                     /gene="tsaD"
                     /locus_tag="IVK78_001177"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP6374660.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25706..26407)
                     /locus_tag="IVK78_001178"
     CDS             complement(25706..26407)
                     /locus_tag="IVK78_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6374661.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            26868..28097
                     /gene="arcA"
                     /locus_tag="IVK78_001179"
     CDS             26868..28097
                     /gene="arcA"
                     /locus_tag="IVK78_001179"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP6374662.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            28188..29207
                     /gene="argF"
                     /locus_tag="IVK78_001180"
     CDS             28188..29207
                     /gene="argF"
                     /locus_tag="IVK78_001180"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP6374663.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            29322..30269
                     /gene="arcC"
                     /locus_tag="IVK78_001181"
     CDS             29322..30269
                     /gene="arcC"
                     /locus_tag="IVK78_001181"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP6374664.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         9921 a         5198 c         6317 g         8913 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa acgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaatcga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaatga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagagat tctgggctgg aaacctgaat acacagatat caaagcgatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc cccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 taccaattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agaagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaact
    20641 tgcttattga agcagctaac tcagtattgg aaaaatggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc gattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgcc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccggca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacact ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggcttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tcgctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt gggaaaatgg
    22201 agtgataccg cagctttttt gtgtttcagg aaggggcaaa atttgcccgc cagcagataa
    22261 tcatgtatta ttgtactagt tttgcctttg gtattttcat cctcagaata aaaaagtatg
    22321 ctacaatgga aggcgagaat gtcatagcag aaaggatcaa atatatgatt acattaggaa
    22381 tagatactgc aaaccaaact ttggcgatcg gtgtggtaga agacgaacaa attcttggac
    22441 aaatacaaac aaatatcaaa cgcaaccaca gtgtaacact gatgcctgca atcgaccaat
    22501 tgtttgcaga tcttcaaata agtccaaaag acattgaccg aatcgcggtt tctgatggtc
    22561 ccggttctta tacagggctt cgtataggtg taacgacagc aaaaacgatt gcttatacat
    22621 tagataaaga gctagtagga gtatctagtc tgaaaaccat cgcggctaat tgcatcggtg
    22681 tggataatat tattgtgccc atgtttgatg ctcgaagaaa aaatgtatac gctggggcat
    22741 accgctggaa agaaaataga ttagaaactg tacttgaaga tgtacatatt tctgcttctg
    22801 atttgttcac acaactacaa aagctgaatg aagctattta tttcgtcggg agtgattgtc
    22861 acaaattctt ctctgaaata aaggaaatat tgcctagtgc gatgattaat acagttccag
    22921 catgggatat tccaagtggt gtaactgtgg ctcaattagg tgctttagtt gaaccagttg
    22981 cagagaaaca agcttttctt cctcgctatt taaaacgagt agaagcagaa gaaaaatggt
    23041 tggagacgca cacacctgga gacgaaagtt atgttgaaaa aatttagagc gtatgccaga
    23101 agcttttttc aagataaaat gatttataca gaaaaaacag tcgaacaaat cgatcgtcgt
    23161 tacgtactaa gagaattgac tacagaagat atcaaagagc tgctggatgt cgaacgagaa
    23221 gtctataatg gcgaattgcc atggacaaaa agtgccttta tatcagaatt gaagtcaccg
    23281 cttcctcatc tatatctagg tatctttgat aatggaagaa tgattggttt tatcggttca
    23341 agagcgttcg gtttagattg ccatatcacg aatattgccg ttcgaccggc atatcagcgt
    23401 aaacgaatcg gttcgctgct tattgacgaa atcgagaact ttgctataat gaatcgctgt
    23461 gagacgatgt cattagaagt gcgtatgagt aatcaagatg cacaaagact ttatcggaaa
    23521 ttggggtttc aagcaacaac gatcaaaaaa agatattaca ctgaaacaaa agaagatgcg
    23581 ttggatatga taaaactttt agaaaatgag tgagattagt tgatttgcag aaaaaaagat
    23641 tttgatgaag cgtacttggc agaatgcgta tggatgatta gcgaaaatgc ttatccacat
    23701 ggttcacctt ggacgaaaat gcagtttttg gcagacatcc aacagcctca ctctgattat
    23761 ctgcttttag ctgaagataa ccagatctgt ggctttattg gatactcgaa agtaatagat
    23821 gaagtcgaga tcacaaatat tgctgttgca gtttcagaac aaggaaaagg gcatgcacgg
    23881 catttgttac aattactgat tacggaacaa ataaaagatt cgctgcatgt ttttttagaa
    23941 gtacgtctat ctaatgaagc agcaagaaaa ctttatgaat cagaaaagtt tcgtatatta
    24001 ggtaaacgga aatcatatta tcgcaatccg acggaagatg cgatcattat gagcagaaag
    24061 ttaaaaacgg agacgatgaa atgaatgaag aattgattct ggcgattgaa tcaagttgtg
    24121 atgaaacgag tgtagcggtc gttagaaatg gaacagagat cttatctaac atcgttgctt
    24181 ctcaaataaa cagtcataaa cgttttggtg gcgtagtacc tgaagtagct agccgccatc
    24241 acgtagaaca aatcacctta tgcttggaag atgcactggt agaagctggt gtgtcggcag
    24301 aagatttatc ggcagtcgct gtcacttatg gaccaggact tgtcggctcg ttgctgattg
    24361 gtatttctgc agctaaggct tttgcttggg cacaccaatt gccgcttatt cctgtaaacc
    24421 atatggcagg gcatatctat gcagcccgtc tggttaaacc attccagttt ccattgatgg
    24481 cacttcttgt tagcggagga catacagaat tagtctatat gcaagaagat ggatcatatg
    24541 aaatcattgg ggagacaaga gatgacgcag caggagaagc ttatgacaaa gttggaagag
    24601 tattaggtct ttcttaccca agcggaaaag aaatcgatca gctagctcat cagggaaaag
    24661 ataattacca ttttccgcga gcgatgatcc atgaagataa ttatgatttc agttttagcg
    24721 gattaaaaag tgcatttatc aatttggtgc ataatgcaca acagcgtgga gaagatctag
    24781 acaaaaatga tttagcagct agctttcaag caagtgtgat cgatgtattg attactaaaa
    24841 cacttcgagc ttgtcaaaat tatccagtca agcaactgat tatagctgga ggtgtagcgg
    24901 ccaatcaagg gttgcgtgaa ggattgcagg atgctttggc aactaaactc cctgaggtag
    24961 aactagtgat tccccctctt cgactttgtg gagataatgc tgcaatgatt ggagcagcag
    25021 cacatgtgga aatgcaaaaa agaaattttg ctagctatca gttgaatgct gatccaagct
    25081 tggttttatc tgaataaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    25141 cccaggaaaa acggcttctc atgtttttga cctggctgtt cttattaccg cgtatgcaag
    25201 cttttgaacg ccgtttaatc aagagttcgt aagcctgtcg tttaactctg attcacaagg
    25261 aacaatttca aaaaacagct cctcatgttt atgaacatta tgacttgtga ccgaagagtt
    25321 gacaggtaca caccgtttaa tcgaaatagg gctgtgacac gtttttgtca aagccctatt
    25381 ttttacatac ttttaatcat tattgttatt ttgctatttt ttacagctta tttatcatta
    25441 tccgttcctc tgattgccct cactgagtaa tcgtttattg tcgaggaata tcgaataatc
    25501 tagatcggat gaggacaaga cgcttcatct agttaatcta acagtatttt ttgcaaacag
    25561 tgaatattac ataactgaaa cacacataga aaatctttga gtcttgtcca gtgatctaaa
    25621 actgccggat ctttcagaag ttttagggag gaaaataagc agtgaccctg atatttatca
    25681 gggtcactgc ttatttttgt tcgcgttatt ctctcatagc tggagtagta gctgtcttac
    25741 agaaagtaaa acgctttttg tcataatcta acagcccgga atcacgtagt tgtttgatta
    25801 cttgtccagc agtttcacgt gtcgtgccgc tattcactgc taattctttt aaggtgatcg
    25861 gataaggaat ggtgatatga ccatcttttg ttggtacgcc caattcttct ttcaaaatga
    25921 ataaattttg ttttacccgg tcaaaggcgc tagaggtcaa tccgatttgg atacgcaatt
    25981 catgtatttc aactacttta gaaatttttt gtatcatata gatcaactgt tcgggatttt
    26041 ttcgtgctaa tttttcatag taggaaacag gaaggtagaa tgtttcaata tcagtcatcg
    26101 cttgagctgt ataagtgtag gtctcttctc caaacattcc tgacatagga aaaaggttat
    26161 ttgtattcac atagtcatag tagtaataag ttgcactttc atcataacgt tctaaacgaa
    26221 cgagtccttt ggtcaagata tacaaccgtt gtctgggatc accatcgtcg aaaaggatct
    26281 gtcctttttt ataagaacga agaacagtat gttccttgat ctgttggaac tcttcatccg
    26341 agaaatgttc aaaatcaggt tgatatctga gttttgaaaa gtgaaaatcg attttattaa
    26401 cacgcattaa aaagtcctct ccttattgtt cactatattt attgtcactt tttttaaaga
    26461 atttgtaaag ttttaacata ccaacattag aaacaagttt taatacttta caaatataat
    26521 ttgttattat gtaaaaaata tacaatggta ttgaataatt tgttttgaag cgaatgattt
    26581 gtgtataaaa aacaacaata atacatatag taaacgctaa caacgataat ataatttatt
    26641 tttttaattt attcaaaaaa attttttcaa tgaataaata tttttttgaa agtgtataca
    26701 gaataaaaaa tgaataaatt acgcttaaaa atagaaaaac gtttaattat ttaattatcg
    26761 aatattaatg tgaggaatgt ctcattttga atgagacaaa attatgaaag tgattacaaa
    26821 aggaggatat cctatacatg taatcaaaaa gaaggaggca acacaacatg gataaaccta
    26881 ttcacgtttt ctctgaaata gggaaattaa agacagtttt actgaaacgg cctggacaag
    26941 aagtggaaaa tttgacacca gatattatgg atcgtctgtt gttcgatgat attccatatc
    27001 ttccgattgc tcaagaagaa catgacaact ttgcgaagac attgcaaaat gaaggcgtcg
    27061 agactcttta cttggaaaaa ttagcggcag aagcgattga cgcaggcaat gtcaaagaac
    27121 aattcttgaa taaaatgttg gatgaatctc atattgcatc gaatgctgta agagagggtc
    27181 ttcatgagtt ccttctatca atggaaacgc aagaaatggt agataaaatc atggctgggg
    27241 ttcgtacaaa agatatcgaa gtacgttcta gcagtctata tgatctgtca gcagacgatg
    27301 actatccatt ttacatggac ccaatgccaa acttgtactt tacacgcgac ccttccgcat
    27361 caatgggaaa tgggatgaca gtcaataaga tgacttttga agcacgtcgt cgtgaatcga
    27421 tgtttacaga atatattttg aaacatcatc caagatttgc aaataaagga atcgaagtat
    27481 ggttggatcg tgaaaatcca gatcatatcg aaggcggaga cgaattgatc ttaagcgata
    27541 aagtagtagc tgttgggatt tctcaacgaa ctaatgcgaa agctttagaa aaattagcac
    27601 gccacctatt cgcaaaaaat tcaggatttg aaaaagtgtt ggcaatcaaa attccgaaca
    27661 accgtgcaat gatgcatttg gataccgtat ttacaatggt agatcacgat aaattcacta
    27721 tccatccagc aattcaaagt aaagacggta agatggatgt ctttacaatc gaaccagatg
    27781 gtgatgatat caagattact cattcagata acttacacga aacgatgaaa gctgctcttg
    27841 gattagatga tcttgtactg atcccaactg gtaatggaga tgagatcgtt gcacctcgag
    27901 aacaatggaa tgatggatca aacacattgg caatcgctcc aggtgttgta gtcacttata
    27961 accgaaatta tgtatcgaac gaattgcttc gtagctatgg catcaaagtg ttagaaatca
    28021 attcaagtga attgtcacga ggtcgtggtg gtccacgctg catgagccaa ccacttgtcc
    28081 gtgaagactt gaagtaattt gtttcctgac ggcagtctta ctgtcagctg ccgtcgggat
    28141 ttcctcttta atcttaaata aaaacaatct aaaggagaat gtttaatatg aaagaatcag
    28201 tattccaagg aagaagctta ttagcagaaa aagactttac aaaagaagaa ttacaatatt
    28261 tgatcgattt ttcagaacac ttgaaagact tgaaaaaacg tggtatccca catcactatc
    28321 tagaaggaaa aaatattgcg cttttatttg aaaaaacatc tacacgtact cgttctgcat
    28381 ttactactgc agcgatcgat ctaggtgcac atcctgaata tctaggtgca aatgatatcc
    28441 aattaggtaa aaaagaatcc acagaagata cagctaaagt actaggacgt atgtttgacg
    28501 gaatcgaatt ccgtggattc agtcaaaaaa tggtagagga actagcagaa ttctctggtg
    28561 ttccagtttg gaatggcttg actgatgaat ggcatccaac acaaatgatc gctgactttt
    28621 taactatcca agaaaacttt ggaacagtag aaggtatcac tgttgcttac tgcggagatg
    28681 gtcgaaacaa tatggctaac tctttattag tcacaggtgc aattttagga gcgaatatgc
    28741 gtatcgttgc accaaaagaa ttacaacctg aagaagaaat cgtcaaaatg gcagaaggct
    28801 ttgctgaaaa atccggtgca caattgatga ttaccgacga tgttgataaa ggtgtagacg
    28861 gagcagacgt cctttattct gatgtatggg tatcaatggg tgaagaagat aaattcgaag
    28921 aacgtatcaa attgttgaaa ccttaccaaa tcaatatgga aatggtagaa aaaacacaca
    28981 acacagatcg tttgatcttc ttgcactgct tgccagcttt ccatgataca aacactgttt
    29041 acggtgaaca aatgaaagaa cgctttggca tcactgaaat ggaagtaaca gatgaagtct
    29101 tccgcagcaa atatgctcgt caatttgacc aagcagaaaa tcgtatgcac tcaatcaaag
    29161 cgatcatggc agcaacatta ggtaacttgt ttatccctcg cgtttaagca taaccacatt
    29221 catctcacga aaaaacgagt cagcattgag ggaaaatcac aaaacgctgg ctcgttttgt
    29281 tatctagaaa attcggtaga actgaagaaa ggacgaatga aatggccaaa cgaaaagtag
    29341 ttgtcgcttt agggggcaat gccatcttgt ctacggatgc aagtgcaaaa gcccaacaag
    29401 aagcgttaat ggaaacagca aagtatttag tgaaatttat cgaacaagga gatgaattga
    29461 tcatctccca cggaaatggt ccgcaagtcg gtaacctatt gattcagcag caagctgctg
    29521 attctgagaa aacaccggca atgcctttag atacatgtgt tgcaatgaca gaaggatcta
    29581 tcggttactg gttacaaaac gccatgggcg aagtactgaa ggaaaaaggg atcgataaag
    29641 atgtggtttc tttagtcact caagttattg tggatgaaaa tgatccttca ttcaaaaatc
    29701 caagtaaacc agtcggtcca ttctacacgg aagaagaagc caaagaacaa atgaatgctg
    29761 actccactgt gacattcaaa gaagatgctg gacgtggatg gagaaaagta gtcgcttccc
    29821 ctaaacccat ctcgattaaa gaagcacgtg tcatcgaaac gctggtcgat caaggagtta
    29881 tcacagtctc tgttggtggc ggtgggattc ctgttgtaga aacagctact ggattagaag
    29941 gacgagaagc cgtaatcgat aaagattttg cttcagaaaa attagccgaa atcatcgatg
    30001 cggatttgct gatcgtattg acaggagtag acaatgtgta tgtcaattac caaaaaccag
    30061 atcaaaagaa attagaaact gttactgttt cagaaatgaa acaatacatt gatgaaaagc
    30121 aattcgctcc aggaagcatg cttccgaaag tagaagcagc tattgcattt gttgaagcaa
    30181 aaccaaacgc taaggcaatc atcacttctt tagaaaatat cgagaatctt ttagcttctg
    30241 aagaaggaac gattatcgta gctgattaat ttgttaagtc tttcgtattt acgaaatgat
    30301 ttatagctat ccaggtggta tttcttctcc tttaggccat ctggatttt
//
LOCUS       DACCCM010000025        29520 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCCM010000025 DACCCM010000000
VERSION     DACCCM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.026"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(233..1081)
                     /locus_tag="IVK78_001182"
     CDS             complement(233..1081)
                     /locus_tag="IVK78_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6374665.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVK78_001183"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVK78_001183"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP6374666.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IVK78_001184"
     CDS             complement(3582..3761)
                     /locus_tag="IVK78_001184"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP6374667.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVK78_001185"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVK78_001185"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6374668.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IVK78_001186"
     CDS             5909..8188
                     /locus_tag="IVK78_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6374669.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IVK78_001187"
     CDS             complement(8281..8928)
                     /locus_tag="IVK78_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374670.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IVK78_001188"
     CDS             complement(9107..9826)
                     /locus_tag="IVK78_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6374671.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IVK78_001189"
     CDS             complement(9957..11108)
                     /locus_tag="IVK78_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP6374672.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IVK78_001190"
     CDS             complement(11325..12065)
                     /locus_tag="IVK78_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6374673.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVK78_001191"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVK78_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP6374674.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IVK78_001192"
     CDS             complement(12449..13042)
                     /locus_tag="IVK78_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6374675.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IVK78_001193"
     CDS             complement(13026..13673)
                     /locus_tag="IVK78_001193"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP6374676.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVK78_001194"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVK78_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP6374677.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVK78_001195"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVK78_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP6374678.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IVK78_001196"
     CDS             complement(15119..15646)
                     /locus_tag="IVK78_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP6374679.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IVK78_001197"
     CDS             complement(15826..16611)
                     /locus_tag="IVK78_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP6374680.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IVK78_001198"
     CDS             complement(16604..17473)
                     /locus_tag="IVK78_001198"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP6374681.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IVK78_001199"
     CDS             complement(17474..18853)
                     /locus_tag="IVK78_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP6374682.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVK78_001200"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVK78_001200"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6374683.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVK78_001201"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVK78_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP6374684.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVK78_001202"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVK78_001202"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP6374685.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVK78_001203"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVK78_001203"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP6374686.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVK78_001204"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVK78_001204"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6374687.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IVK78_001205"
     CDS             complement(22811..23035)
                     /locus_tag="IVK78_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6374688.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IVK78_001206"
     CDS             complement(23088..24050)
                     /locus_tag="IVK78_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP6374689.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IVK78_001207"
     CDS             complement(24060..24500)
                     /locus_tag="IVK78_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6374690.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IVK78_001208"
     CDS             24806..25771
                     /locus_tag="IVK78_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6374691.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IVK78_001209"
     CDS             complement(25828..26058)
                     /locus_tag="IVK78_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP6374692.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IVK78_001210"
     CDS             complement(26144..27031)
                     /locus_tag="IVK78_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6374693.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IVK78_001211"
     CDS             complement(27120..28343)
                     /locus_tag="IVK78_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6374694.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IVK78_001212"
     CDS             complement(28360..29439)
                     /locus_tag="IVK78_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6374695.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8204 a         6449 c         5076 g         9791 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
//
LOCUS       DACCCM010000026        29154 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCCM010000026 DACCCM010000000
VERSION     DACCCM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.027"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1030)
                     /locus_tag="IVK78_001213"
     CDS             complement(<1..1030)
                     /locus_tag="IVK78_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6374696.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IVK78_001214"
     CDS             complement(1112..1801)
                     /locus_tag="IVK78_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6374697.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVK78_001215"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVK78_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP6374698.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IVK78_001216"
     CDS             complement(2328..2831)
                     /locus_tag="IVK78_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP6374699.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVK78_001217"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVK78_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP6374700.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVK78_001218"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVK78_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP6374701.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVK78_001219"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVK78_001219"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP6374702.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVK78_001220"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVK78_001220"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP6374703.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IVK78_001221"
     CDS             complement(6443..7462)
                     /locus_tag="IVK78_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6374704.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IVK78_001222"
     CDS             complement(7635..8522)
                     /locus_tag="IVK78_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6374705.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVK78_001223"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVK78_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP6374706.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVK78_001224"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVK78_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP6374707.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVK78_001225"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVK78_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6374708.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IVK78_001226"
     CDS             9753..10397
                     /locus_tag="IVK78_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6374709.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IVK78_001227"
     CDS             complement(10453..10956)
                     /locus_tag="IVK78_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6374710.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IVK78_001228"
     CDS             11553..12518
                     /locus_tag="IVK78_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6374711.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IVK78_001229"
     CDS             complement(12617..13789)
                     /locus_tag="IVK78_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374712.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IVK78_001230"
     CDS             complement(13776..14921)
                     /locus_tag="IVK78_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374713.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IVK78_001231"
     CDS             complement(14935..15867)
                     /locus_tag="IVK78_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374714.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IVK78_001232"
     CDS             15984..17063
                     /locus_tag="IVK78_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6374715.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IVK78_001233"
     CDS             17060..17689
                     /locus_tag="IVK78_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6374716.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IVK78_001234"
     CDS             complement(17799..18026)
                     /locus_tag="IVK78_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6374717.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IVK78_001235"
     CDS             complement(18129..20717)
                     /locus_tag="IVK78_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP6374718.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IVK78_001236"
     CDS             complement(20737..21396)
                     /locus_tag="IVK78_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6374719.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVK78_001237"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVK78_001237"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP6374720.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IVK78_001238"
     CDS             complement(22169..23035)
                     /locus_tag="IVK78_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6374721.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IVK78_001239"
     CDS             23144..23785
                     /locus_tag="IVK78_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP6374722.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVK78_001240"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVK78_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP6374723.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IVK78_001241"
     CDS             complement(25226..26125)
                     /locus_tag="IVK78_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6374724.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IVK78_001242"
     CDS             complement(26127..26924)
                     /locus_tag="IVK78_001242"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP6374725.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IVK78_001243"
     CDS             complement(26902..27606)
                     /locus_tag="IVK78_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP6374726.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IVK78_001244"
     CDS             27737..28312
                     /locus_tag="IVK78_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP6374727.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IVK78_001245"
     CDS             28430..29107
                     /locus_tag="IVK78_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6374728.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8582 a         6173 c         5242 g         9157 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa aggctctttg tcaaatgatg ttggtaaaga gaaatcttag aata
//
LOCUS       DACCCM010000027        29120 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCCM010000027 DACCCM010000000
VERSION     DACCCM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.028"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            271..1140
                     /locus_tag="IVK78_001246"
     CDS             271..1140
                     /locus_tag="IVK78_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP6374729.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1349..2374
                     /locus_tag="IVK78_001247"
     CDS             1349..2374
                     /locus_tag="IVK78_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6374730.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2413..3363
                     /gene="manA"
                     /locus_tag="IVK78_001248"
     CDS             2413..3363
                     /gene="manA"
                     /locus_tag="IVK78_001248"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP6374731.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3457..5160
                     /locus_tag="IVK78_001249"
     CDS             3457..5160
                     /locus_tag="IVK78_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6374732.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5162..7195
                     /locus_tag="IVK78_001250"
     CDS             5162..7195
                     /locus_tag="IVK78_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6374733.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7206..7646
                     /locus_tag="IVK78_001251"
     CDS             7206..7646
                     /locus_tag="IVK78_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6374734.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7846..8973)
                     /locus_tag="IVK78_001252"
     CDS             complement(7846..8973)
                     /locus_tag="IVK78_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374735.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8976..9653)
                     /locus_tag="IVK78_001253"
     CDS             complement(8976..9653)
                     /locus_tag="IVK78_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374736.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9917..11683
                     /locus_tag="IVK78_001254"
     CDS             9917..11683
                     /locus_tag="IVK78_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP6374737.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11698..12135
                     /locus_tag="IVK78_001255"
     CDS             11698..12135
                     /locus_tag="IVK78_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6374738.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12148..13299
                     /locus_tag="IVK78_001256"
     CDS             12148..13299
                     /locus_tag="IVK78_001256"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP6374739.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13361..14059)
                     /locus_tag="IVK78_001257"
     CDS             complement(13361..14059)
                     /locus_tag="IVK78_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP6374740.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14052..15404)
                     /locus_tag="IVK78_001258"
     CDS             complement(14052..15404)
                     /locus_tag="IVK78_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP6374741.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15627..17027)
                     /locus_tag="IVK78_001259"
     CDS             complement(15627..17027)
                     /locus_tag="IVK78_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP6374742.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17188..17925
                     /locus_tag="IVK78_001260"
     CDS             17188..17925
                     /locus_tag="IVK78_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP6374743.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18050..18529
                     /locus_tag="IVK78_001261"
     CDS             18050..18529
                     /locus_tag="IVK78_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP6374744.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18526..19293
                     /locus_tag="IVK78_001262"
     CDS             18526..19293
                     /locus_tag="IVK78_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374745.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19523..20092
                     /gene="lepB"
                     /locus_tag="IVK78_001263"
     CDS             19523..20092
                     /gene="lepB"
                     /locus_tag="IVK78_001263"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6374746.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20196..20798)
                     /locus_tag="IVK78_001264"
     CDS             complement(20196..20798)
                     /locus_tag="IVK78_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6374747.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20904..21373
                     /locus_tag="IVK78_001265"
                     /pseudo
     CDS             20904..21373
                     /locus_tag="IVK78_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21479..22063
                     /locus_tag="IVK78_001266"
     CDS             21479..22063
                     /locus_tag="IVK78_001266"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP6374748.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22066..23139
                     /gene="prfA"
                     /locus_tag="IVK78_001267"
     CDS             22066..23139
                     /gene="prfA"
                     /locus_tag="IVK78_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP6374749.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23132..23971
                     /gene="prmC"
                     /locus_tag="IVK78_001268"
     CDS             23132..23971
                     /gene="prmC"
                     /locus_tag="IVK78_001268"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP6374750.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23964..24143
                     /locus_tag="IVK78_001269"
     CDS             23964..24143
                     /locus_tag="IVK78_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374751.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24165..25190
                     /locus_tag="IVK78_001270"
     CDS             24165..25190
                     /locus_tag="IVK78_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP6374752.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25365..26609
                     /locus_tag="IVK78_001271"
     CDS             25365..26609
                     /locus_tag="IVK78_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP6374753.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26762..27709
                     /locus_tag="IVK78_001272"
     CDS             26762..27709
                     /locus_tag="IVK78_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6374754.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27843..28472
                     /gene="upp"
                     /locus_tag="IVK78_001273"
     CDS             27843..28472
                     /gene="upp"
                     /locus_tag="IVK78_001273"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP6374755.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28611..28853)
                     /locus_tag="IVK78_001274"
     CDS             complement(28611..28853)
                     /locus_tag="IVK78_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374756.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28974..29046
                     /locus_tag="IVK78_001275"
     tRNA            28974..29046
                     /locus_tag="IVK78_001275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29007..29009,aa:Thr,seq:cgt)
BASE COUNT         9884 a         5151 c         5955 g         8130 t
ORIGIN      
        1 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
       61 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
      121 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      181 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      241 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      301 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      361 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      421 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      481 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      541 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      601 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      661 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      721 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      781 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      841 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      901 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      961 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
     1021 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1081 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1141 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1201 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1261 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1321 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1381 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1441 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1501 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1561 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1621 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1681 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1741 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1801 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1861 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1921 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1981 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     2041 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2101 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2161 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2221 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2281 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2341 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2401 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2461 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2521 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2581 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2641 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2701 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2761 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2821 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2881 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2941 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     3001 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3061 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3121 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3181 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3241 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3301 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3361 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3421 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3481 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcacct aaatgaaatg
     3541 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3601 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3661 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3721 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3781 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3841 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3901 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3961 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     4021 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4081 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4141 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4201 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4261 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4321 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4381 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4441 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4501 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4561 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4621 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4681 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4741 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4801 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4861 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4921 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4981 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     5041 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5101 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5161 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5221 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5281 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5341 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5401 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5461 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5521 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5581 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5641 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5701 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5761 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5821 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5881 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5941 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     6001 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6061 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6121 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6181 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6241 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6301 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6361 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6421 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6481 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6541 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6601 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6661 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6721 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6781 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6841 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6901 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6961 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     7021 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7081 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7141 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7201 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7261 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7321 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7381 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7441 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7501 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7561 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7621 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7681 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7741 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7801 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7861 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7921 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7981 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     8041 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8101 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8161 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8221 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8281 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8341 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8401 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8461 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8521 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8581 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8641 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8701 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8761 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8821 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8881 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8941 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     9001 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9061 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9121 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9181 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9241 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9301 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9361 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9421 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9481 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9541 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9601 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9661 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9721 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9781 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9841 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9901 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9961 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
    10021 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10081 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10141 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10201 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10261 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10321 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10381 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10441 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10501 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10561 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10621 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10681 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10741 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10801 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10861 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10921 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10981 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    11041 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11101 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11161 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11221 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11281 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11341 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11401 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11461 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11521 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11581 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11641 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11701 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11761 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11821 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11881 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11941 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    12001 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12061 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12121 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12181 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12241 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12301 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12361 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12421 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12481 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12541 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12601 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12661 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12721 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12781 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12841 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12901 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12961 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    13021 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13081 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13141 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13201 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13261 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13321 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13381 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13441 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13501 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13561 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13621 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13681 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13741 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13801 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13861 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13921 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13981 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    14041 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14101 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14161 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14221 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14281 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14341 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14401 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14461 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14521 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14581 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14641 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14701 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14761 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14821 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14881 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14941 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    15001 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15061 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15121 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15181 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15241 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15301 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15361 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15421 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15481 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15541 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15601 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15661 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15721 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15781 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15841 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15901 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15961 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    16021 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16081 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16141 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16201 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16261 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16321 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16381 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16441 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16501 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16561 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16621 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16681 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16741 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16801 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16861 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16921 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16981 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    17041 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17101 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17161 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17221 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17281 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17341 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17401 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17461 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17521 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17581 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17641 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17701 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17761 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17821 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17881 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17941 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    18001 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18061 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18121 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18181 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18241 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18301 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18361 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18421 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18481 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18541 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18601 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18661 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18721 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18781 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18841 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18901 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18961 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    19021 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19081 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19141 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19201 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19261 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19321 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19381 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19441 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19501 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19561 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19621 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19681 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19741 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19801 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19861 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19921 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19981 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    20041 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20101 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20161 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20221 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20281 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20341 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20401 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20461 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20521 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20581 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20641 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20701 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20761 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20821 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20881 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20941 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    21001 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21061 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21121 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21181 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21241 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21301 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21361 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21421 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21481 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21541 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21601 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21661 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21721 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21781 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21841 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21901 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21961 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    22021 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22081 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22141 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22201 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22261 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22321 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22381 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22441 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22501 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22561 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22621 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22681 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22741 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22801 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22861 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22921 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22981 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    23041 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23101 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23161 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23221 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23281 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23341 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23401 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23461 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23521 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23581 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23641 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23701 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23761 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23821 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23881 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23941 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    24001 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24061 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24121 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24181 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24241 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24301 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24361 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24421 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24481 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24541 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24601 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24661 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24721 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24781 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24841 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24901 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24961 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    25021 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25081 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25141 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25201 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25261 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25321 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25381 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25441 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25501 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25561 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25621 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25681 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25741 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25801 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25861 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25921 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25981 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    26041 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26101 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26161 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26221 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26281 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26341 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26401 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26461 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26521 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26581 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26641 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26701 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26761 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26821 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26881 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26941 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    27001 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27061 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27121 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27181 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27241 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27301 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27361 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27421 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27481 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27541 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27601 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27661 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27721 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27781 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27841 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27901 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27961 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    28021 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28081 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28141 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28201 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28261 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28321 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28381 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28441 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28501 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28561 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28621 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28681 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28741 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28801 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28861 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28921 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28981 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    29041 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29101 ttttgtttaa aggaaataaa
//
LOCUS       DACCCM010000028        28681 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCCM010000028 DACCCM010000000
VERSION     DACCCM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.029"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(20..1186)
                     /locus_tag="IVK78_001276"
     CDS             complement(20..1186)
                     /locus_tag="IVK78_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6374757.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(1198..1551)
                     /locus_tag="IVK78_001277"
     CDS             complement(1198..1551)
                     /locus_tag="IVK78_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6374758.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1679..2701
                     /locus_tag="IVK78_001278"
     CDS             1679..2701
                     /locus_tag="IVK78_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP6374759.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(3140..3574)
                     /locus_tag="IVK78_001279"
     CDS             complement(3140..3574)
                     /locus_tag="IVK78_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374760.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(3684..5543)
                     /locus_tag="IVK78_001280"
     CDS             complement(3684..5543)
                     /locus_tag="IVK78_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6374761.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(5661..6820)
                     /locus_tag="IVK78_001281"
                     /pseudo
     CDS             complement(5661..6820)
                     /locus_tag="IVK78_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(7127..8551)
                     /gene="araA"
                     /locus_tag="IVK78_001282"
     CDS             complement(7127..8551)
                     /gene="araA"
                     /locus_tag="IVK78_001282"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP6374762.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAEEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(8567..9265)
                     /locus_tag="IVK78_001283"
     CDS             complement(8567..9265)
                     /locus_tag="IVK78_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6374763.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(9222..10883)
                     /locus_tag="IVK78_001284"
     CDS             complement(9222..10883)
                     /locus_tag="IVK78_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP6374764.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            11101..12501
                     /locus_tag="IVK78_001285"
     CDS             11101..12501
                     /locus_tag="IVK78_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP6374765.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(12753..13832)
                     /locus_tag="IVK78_001286"
     CDS             complement(12753..13832)
                     /locus_tag="IVK78_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6374766.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(13918..14877)
                     /locus_tag="IVK78_001287"
     CDS             complement(13918..14877)
                     /locus_tag="IVK78_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP6374767.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(14889..16412)
                     /locus_tag="IVK78_001288"
     CDS             complement(14889..16412)
                     /locus_tag="IVK78_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6374768.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(16422..16985)
                     /locus_tag="IVK78_001289"
     CDS             complement(16422..16985)
                     /locus_tag="IVK78_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6374769.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(16991..17854)
                     /locus_tag="IVK78_001290"
     CDS             complement(16991..17854)
                     /locus_tag="IVK78_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6374770.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(17868..18764)
                     /locus_tag="IVK78_001291"
     CDS             complement(17868..18764)
                     /locus_tag="IVK78_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6374771.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(18939..20210)
                     /locus_tag="IVK78_001292"
     CDS             complement(18939..20210)
                     /locus_tag="IVK78_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6374772.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(20200..21717)
                     /locus_tag="IVK78_001293"
     CDS             complement(20200..21717)
                     /locus_tag="IVK78_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6374773.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            21915..22805
                     /locus_tag="IVK78_001294"
     CDS             21915..22805
                     /locus_tag="IVK78_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP6374774.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(22878..23702)
                     /gene="nadE"
                     /locus_tag="IVK78_001295"
     CDS             complement(22878..23702)
                     /gene="nadE"
                     /locus_tag="IVK78_001295"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP6374775.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(23714..25186)
                     /locus_tag="IVK78_001296"
     CDS             complement(23714..25186)
                     /locus_tag="IVK78_001296"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP6374776.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(25823..26185)
                     /locus_tag="IVK78_001297"
     CDS             complement(25823..26185)
                     /locus_tag="IVK78_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6374777.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            26408..27160
                     /locus_tag="IVK78_001298"
     CDS             26408..27160
                     /locus_tag="IVK78_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6374778.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(27300..28223)
                     /locus_tag="IVK78_001299"
     CDS             complement(27300..28223)
                     /locus_tag="IVK78_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6374779.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFLDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(28238..28510)
                     /locus_tag="IVK78_001300"
     CDS             complement(28238..28510)
                     /locus_tag="IVK78_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374780.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         8479 a         5985 c         4883 g         9334 t
ORIGIN      
        1 tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct tcataaagag
       61 aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct gctgcataaa
      121 caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc agcagaacta
      181 ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga gagatcaagc
      241 gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc atgaatggac
      301 ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct aagtaaggga
      361 tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa ccaatgaccg
      421 caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt cctcttatat
      481 aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt tgcatgcgtg
      541 tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat agataaaata
      601 agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg acaaaacttc
      661 caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc gaattcatga
      721 acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa
      781 tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg atgaagctca
      841 ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt agtgcaacaa
      901 ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt tcaaagtaat
      961 caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc agacctacga
     1021 ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga
     1081 tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt aaaaaccagc
     1141 gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt cctcctgtta
     1201 gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat gaagatagat
     1261 tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata cgtgagctac
     1321 tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata cctgatcatc
     1381 aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata cttcttgaat
     1441 caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca gcatgggttc
     1501 ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca ttgcactctc
     1561 tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg tgtggaataa
     1621 ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat cagctcatat
     1681 gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat
     1741 cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg aagaaaatag
     1801 ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta gtgtggatgg
     1861 attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg taacagagtc
     1921 tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat acggggcgaa
     1981 ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac tgaaagcagc
     2041 agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc atgcgcatgt
     2101 ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg gtacagatcg
     2161 tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta ccttcactgc
     2221 agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat tcgcttattt
     2281 atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa caggtgcttt
     2341 tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa atggaggggc
     2401 agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag gacataactc
     2461 gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc aaatcatctc
     2521 aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt ttgtcgtgtg
     2581 tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa caggattact
     2641 ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta
     2701 gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac gggaagaact
     2761 aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag aggaatatca
     2821 gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg tagaaaacta
     2881 atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt ctatcctgat
     2941 gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa aggagtattt
     3001 actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt atcataaagg
     3061 attttgatca ctctatcata aaggtaatat tacatttata ttagataact ctagttttta
     3121 ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt tggatggcag
     3181 ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat agatcttcat
     3241 ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct acatatgaaa
     3301 gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac
     3361 tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac aggatgatct
     3421 cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg tttctgatca
     3481 cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg atttgatcga
     3541 tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt tctccattgt
     3601 tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa aaaatttaat
     3661 ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc taaactatat
     3721 tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat ctcttgttgc
     3781 gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag ttctcgttca
     3841 aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt tgttgtctga
     3901 ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc
     3961 gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc atgcaatgtt
     4021 tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg atccccccaa
     4081 aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg cttcagtgcc
     4141 gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc acttccatct
     4201 gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat atgtgtctgc
     4261 atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt cattacttct
     4321 ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt ataggtcatc
     4381 gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc tcttttatac
     4441 atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa
     4501 acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt ggtgagttgc
     4561 gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt tgctgttcca
     4621 ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac ttcataataa
     4681 tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc tgagaaactt
     4741 tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt tttttcatcg
     4801 gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata tccttcaggt
     4861 tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc agtgttatag
     4921 tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc ttttcgcaac
     4981 aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc cacaagcagt
     5041 gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa catgtaccag
     5101 caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag catagtgccg
     5161 gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg ataaatcggt
     5221 tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat ggctacgaca
     5281 tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat aagaataatc
     5341 agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac
     5401 gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact aaaaacaaca
     5461 agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa
     5521 agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata aaagaatgtt
     5581 gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa gaattaatga
     5641 aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg gacctttcgt
     5701 actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa atcagtactg
     5761 tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat atctgcgatc
     5821 gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt tttcttttga
     5881 taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg ataattgacc
     5941 gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc caaacgtccg
     6001 ctgtctgcca aagcttcctg agtcgatccc ctagatgagg caacataagt atacggtcat
     6061 tttctaagaa ctcttcttct ggaaaatcag tcaagtattt agccagatgt tgtttttcca
     6121 atgcttgcat aacagcatat ttatcaacaa tttcacttct tcctacattg ataagtacag
     6181 catctttttt cattttttct atattcgcct gatcgatcaa tccttttgta tcttctgtca
     6241 acggcagcat gactacgatg taatccgagg tagaaagaac tgtttctaaa tctgcttgcc
     6301 gaacataatc caactgggca tcgcggatag aataacccaa gacatccatc cctaagctat
     6361 agcaacttag cgctactttt gtgcctatcg ctccaagacc aagaagaccg atggttttgc
     6421 cttgcaattc tcgtcccaca taagcacttc ttttgttttc tgcctgttct aaaatattag
     6481 gacctgttag tgtttgtacc atacgacttg cttcgattat cggacgggaa gataagagca
     6541 aacagcacaa aacaagctct tttactgcat tagcatttac acctggtgta ttcatgacga
     6601 tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg
     6661 aaattgctag caaatctggt gtaagccatt catctttcac tttcgtacta cgaataacta
     6721 ttccttcggc tggcaaatca tctattattt tgaaatattc cccttgtaac ttttctatct
     6781 cttgttctgg aatagcatca atcaaatgaa cgtgatacat aaaaccccct ccttactaac
     6841 aagtatctcg aaatttctgc aattagacaa ctgtttacca tttgacttcc tagaagcaaa
     6901 aagagcagtt agagcagagt aggctgagtg ataaaaataa ggctatgaca agttactgcc
     6961 ataagcctta ttttcgagag caaaatattg agggtgagat atcttgataa atggtgttcc
     7021 gttagctgac attgataata agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa
     7081 tctcttctta tttcttaaaa ctaagtggct ttttcacaac ctctttttat ctaataacca
     7141 ctgtttccaa ctcaactaat tttgcccacg tttcaatttg atctgttgtt agatttagcg
     7201 agacaacggt atgatgaccg ccacctgcct gaatccatga atggacacct tcatggaaat
     7261 tcggtttagt tttccataac acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt
     7321 caaaggcttc tacttcattg atcagcagtt tataatgcgt accgaaatct gccatcgata
     7381 cgactacccc ttctcctgct ttcccatcaa atacaagacg cgccggatct tctctatctc
     7441 ccattgataa aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc ggatctactt
     7501 ccatcatatg ggattccaag attgcttctt ggccactagc tagctcgtaa gtataatctt
     7561 ccatgaatcc tgtatctttc cctcgagcca tgattttcag caaccgatca atcgctgcag
     7621 ttttccagtc gccttcctca gcaaaaccgt atccttctgc catcaaacgt tgtacagcta
     7681 gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc
     7741 ctccttcttc taagaaacgg cgaataccga tttcctgctg agcttgtact tttacatgtt
     7801 cctcccatgt cttttcttca taatcaccat agtcgaaggt atacaattcc ttgtactctt
     7861 caaataattg atcgacttct tcgtctgata cgcgatcgat tacttgtaca agatctccga
     7921 taccaaaata atcaacagtc caaccaaatt gaatctgtgc ttccactttg tctccttctg
     7981 tgacacccac gtttctcatg ttgtcaccaa aacgagccac ttttattcca aaactttcat
     8041 tataggctac tgcaacatcc atccaatcag caatctcttt ttggatctca ggtcgtttcc
     8101 agtagccaac gacgatttta ttttgtttat ttaatcgagc attgatgaac ccatattcgc
     8161 ggtcaccatg agcggattga tttaaattca tgaaatccat atcgatcgtt ttccaaggaa
     8221 tgctttcgtt atattgcgta gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc
     8281 ggatccacat ttttgctggt gagaaagtat gcatccaagt gatcacacct gctacttctt
     8341 ctcgataatt gacctctttc atgatttttg tgatcgtatc agcagtaact gctaattctt
     8401 gaaggcggat tggataaggc aattggccat tttcattcag ttcatcgacc atttcttgtg
     8461 catgtttctt tacttctcgc agtgcttctt ccccatataa atgctgtgat cctacaacaa
     8521 accaaaattc tttttctcca atattcaaca taattagcca ctccttttat ttgttttgtc
     8581 cgtaatacgc attttttccg tgttttcgta aataatgttt atctaaaatc cgttgaggca
     8641 gctcttctga aaatgaattg agctgtctgg taaataaatt catcttgcat acttcatcta
     8701 gtacgactgc gttcattacc gcactttgcg catctttccc ccaagtaaat ggaccgtgtc
     8761 cgtgtaaaag tacccctgga attgctaagg gatcaatttt gcgttctttg aatgtttcta
     8821 tgataacttt tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc
     8881 gagcacaagg taccgatcca taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat
     8941 ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg attcctccga
     9001 tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt atccgaagag ggattgagtt
     9061 taccttcaat gatatgattg tccaagtcca caacgaccat gtcgtctgct gtcatctctt
     9121 cataactgac tccgctaggt ttgataacga aatatcgtgt ttcaggatca aaagcgctca
     9181 catttcccca tgtatatttg acgagtccgt gtttaggcaa gtcaagattt gcttggtata
     9241 cttcttcttt cagttgttcc agcattttct atcactcctt tttcttgcaa ttgatgaatc
     9301 gctgtttttt caatgtcgat cccctcacgg taacgtctca taaatatctg atagcctgct
     9361 atttcttctg ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac gtcttctgcc
     9421 aagaagtttt ctaaagactt cccatgtcga tccatcatat aggctgctaa taacgcgatt
     9481 ccccacgctc caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc catagcagat
     9541 gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga
     9601 tcaatttgga cattctcaga tttcaatata tccattccga ttctcattgc accaaaagca
     9661 ctggctaaat ggatccgcat gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca
     9721 aataatggcc ttccttcatt tactcctgta atgttttcac cggaaaaata accataagaa
     9781 agtaatcccc cgcaatccgt atcgccttct agcgctttgt tgaataatac cgtgaataat
     9841 ttatctctgc ttatctccat acccaaagaa ttggtaaatt cttcaaaaat actgacccaa
     9901 gcattgatat ccgaagagca gttattcgta tgaaccatgc ctactagttc tcctgaaggt
     9961 gtagtgacca tgtcgatttc cggatggaca ttttctagtt ctttttctaa tacgatcata
    10021 gcgaaggcgg aagtaccagc cgaaatgttc cctgtccctt tggctacact gttcgtagcg
    10081 accataccgg ttcctgcatc cccttctggc ggacaaaccg ggattcctgg tctcaaattt
    10141 cccgtagggt cgatcagata agcccctgat tcgcttaact tgccagcttc ttctcctgct
    10201 actagaactt taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac
    10261 tgaccaaaaa tatttttcat ttcttgatgg taggttttcg ttgaaggatc gataggaaac
    10321 atgcctgatg catctcctac gcctagtact ttctcaccag taagctgcca atggacataa
    10381 cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt cttctttatt caaaatagat
    10441 tgatacaaat gagcgatact ccatctctgc ggaatattgt attgaaattt ttccgttaat
    10501 attcgttctg cttcttcagt gattgtattt cgccaagtac gaaatggtac tagtaactgt
    10561 tccttgctat caaatgctag ataaccgtgc atcattgcac tgaacccaat cgacccgatt
    10621 gtcgtaagat tctcgccata gatttcgaat acttctgctg ccatttttcg gtaactgacg
    10681 cgcagtcctt tccaaatatc ttctaatgaa taggtccaga taccattttc tagtttgttt
    10741 tcccactcat aactaccagc ggcaatagga gtaaaagatg aatcaatcaa tacagctttg
    10801 atcctagttg atccaaactc aatccctaaa gatgtacgac catctatgac atctaacata
    10861 ctttgcgcat ttacttcctt cataatgatt taactccctc cttctcgtca aacctgatca
    10921 aaccgtcttt ttagtagcgc ttacaatgat tattgtatac ttttgtacgt acatgtcaag
    10981 cagttattta caagaataca ataaaaaaca cttgttttgt acgtacataa gttataaaat
    11041 gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat tctagaagga gagaatactt
    11101 atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc atttggcggc
    11161 attctatttg gatatgatat tggtgttatg accggcgcct tacctttttt acagcatgat
    11221 tggggtttgg caggtaaagc ttctttgata ggatggatca cgtcttcggt catgttaggt
    11281 gcgatccttg gcggctcttt atctggtctt ctttctgata agcttggacg acgaaaaatg
    11341 attcttcttt ctgcacttat ttttatggct ggttctgtcc tttctgcatc tgcccctcac
    11401 aatggttctt attttctgat tgcagcaagg attcttctag gtttagctgt tggcgctgca
    11461 tctgccctag ttcctgctta tatgtctgaa atggctcctg ctagactaag gggaagattg
    11521 tcagggatca atcaagtgat gattgcttct ggcatgctcc tttcctatgt tgcagattat
    11581 ttgctaaaag ggttgcctga aacgatggct tggcgcgtga tgttagggct cgctgctgtc
    11641 cccgcgctga ttttattttt cggtgtttta gctctaccag agtcaccgcg ttttcttatg
    11701 caatctggcc gcttggaaga ggcaaaaaga gtattgaact atattcggac accaaaagaa
    11761 gctgaacagg aattcgaaca aatccagctg aatgtcaaac aagaaaagac aaccggaact
    11821 tcttggcata ctttgttttt agaaaaatat cgttccttag tcattgccgg tattggtgta
    11881 gcagtattcc agcaatttca aggagctaat gctatttttt actatattcc gctgatcgta
    11941 gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt ggccgatcat ccaaggaatc
    12001 attttagttg ctggttcttt gctctttttg gttattgcag acaagttcaa tcggcgaact
    12061 ttattgaaaa taggcggtag cgtgatggga ctttctttca tcctaccagc tgttttagga
    12121 actgtactgg atgctcatac aaattcgttg ttgattctct tgtttttatg tatttatgtc
    12181 gcattttatt cttgcacatg ggctccactg acatgggtaa tcgtaggcga aatattccca
    12241 ctagctgtcc gcgggcgagc atctggccta gcctcttctt ttaattggat tggttctttc
    12301 ctagttggct tgctttttcc tgtgatgact gcttctatgt ctcaagaaat cgttttcggc
    12361 atttttggga tcatctgctt tttaggggtt ttatttattc aagagatcgt accagaaact
    12421 cgtgggaaat ctttagagga aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt
    12481 atttctatcc atcattctta aagaggttgt gacagaagtg gtcagcttca agaaataaga
    12541 aggaaccgct tagtttccag tcacaaggaa caatttcaaa aaacagcttc tcatgtttga
    12601 tgaatattgt gacttgtgac cagttggtag gagaacaccg tttagtggag aggtggtgaa
    12661 agaaacgttc agccttgagc gatgacaaaa gctctcacaa acattgcgac gagtaaccgt
    12721 aggagcaata agaggcggta aaaacccgtc tcttattttt cgttttcttt cactgtttcc
    12781 cgttcaacta gtacaggttc atagagaata tttggctgtg cctgtccttt ttcaatcgtt
    12841 gtgacgatcc accgtgcggc atcttgtccc aacttttctt ttggatgcgt caatgttgtc
    12901 aattttttat tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat cgataggtca
    12961 tctggaatat gatagccatt ttctgtcaac ttatctagca ttaaactagc tatttgatcg
    13021 ttatagcaaa tgatgccatc taaagcttgt tcttctttca accgttgtac tgttttttca
    13081 gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca
    13141 ttatactgtt cacacgcgct gataaaacct ttcatcctgt attttccttg aagatcgtcg
    13201 atttttgtga tgaacaacag acgctgatgt ccttttttta tcaaatattc tgctgccata
    13261 aaaccgactt tcacatcatc taaacaaata taagaggcat ttaactcttc ataagcagca
    13321 ttgatcatca ccatcggaat gccttgttct ctcaaagcaa cataaagtgc taagtttggg
    13381 ttatactgat tacttttcgt aggctcaacg atcaaaccat ctaccccata agcaatcatt
    13441 ttcttcaaac actctttctc caattcgtga tcgttattcg tactggctaa aagcaatgaa
    13501 tacccagatt ctcgtaattc attttcgatt cctcggataa tcgacggaaa aatataatca
    13561 gagagatatg tggtgatgac accgatcgtt tttgttctgt tcaatggtgt tttttctttc
    13621 ttatagctgt catctacata tgtgcccgac cctttttctt tacgcagata gccctcattt
    13681 accaacacag cgattgcttg ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc
    13741 ttgaattctg agggaatggt tgtatttact tcgtattcct cacgaaggat cttttgtttt
    13801 atctcttctg caatatcttg atattttgcc atttgccttg ccccttttta tttgtacgta
    13861 catatatcat aacgagtctg caaggtttat tcaagcaaaa atcaaggttt cttgtgctca
    13921 taaggcatac ggaacaggtt caccaaattc tggaaatcct tgctcattcc acgtaaagca
    13981 ttgcgcgttc gcgtgccgat taggattgtc taacggatcc cctttttggt tcttctctgg
    14041 tcgagcatga tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg
    14101 accaggacca aattgcttat ttttttcaga agaaacgaat actggctccg ataatttatg
    14161 ccatgattct tttaataaaa gattcttgtg ctcatcagcc cacagcaggc ccatggcata
    14221 gttttcatct gttgcactac cagaatacgt aatgaatatt ttcccattgc gagtaaggac
    14281 tgctggacct tcatttactg aaaaaccccg tttttcccat tcatactcag gtatagaaag
    14341 gaggatctgc ttccccttta acgtccaagg attatccatt tcggatagat aaagattaga
    14401 attcccagta atttcaggat cttgttgggc ccacacataa tatagctttc cttgagaaga
    14461 gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc ccttttctat
    14521 ccaatctgta ttcattggat ctatatgctg attttcaagt acgaacatgc ggtgatgatg
    14581 agattcatca cgtatagttt tatgattgct tgcagcaaag taaatatacc attttccatt
    14641 gatataatgg acttcaggtg cccagatcaa ttcactcatc ggtccttctt catgagcctg
    14701 ccatacaatc aggcttgcag catgttctaa ttcattcagt gactttgccc ttcttaattc
    14761 aatcacttgg tagcctggaa ccgatcctgt aaaataataa taaccatctg tatgtttata
    14821 tacccaagga tctgcccgtt gccgaacgat aggattatta taaagtgtct tcatcataac
    14881 aaatttcctc acattttctt tattctaatt acattccaag agtatttttc tatccctaca
    14941 tttaattcgt catgtttttc tattgaaaaa tagtttattt caggttttat tttttctggt
    15001 tcttcacgtg tatttttgtc caacagatcg ctactggtta atgtgtagtg ctctccttga
    15061 tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc
    15121 aagaaaatag tcagctgatt gccgttatct acgatcacac tgtccacata aggaacattt
    15181 tttatctttt cacaatcata aacagggcaa atgatttgtt gtttcaatac ttgaccttta
    15241 gcatatttcg ataagtgttg catcacataa taagttggat tcctccatgc ttgtccttta
    15301 gcatctgtca gtattaaagg tattgtgttt actaacaatg attgacaact aatctttacg
    15361 cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa ataataatgt atcttccata
    15421 gtaaagtaac accaatcgat gggactaccg acttcaaaat ccctatgtct tttttccggt
    15481 tgggcatgta cattccattc atcaaacgct agatacattg ttttctcact tctcaaatat
    15541 ccttttacat aatcacaagt agcaacgatt tcttcaattt gacgatcgaa tcgataagct
    15601 tttgcaaggt atgtcgctgt atcatcacgc tcatttttag gaggtttagt caagtcttct
    15661 tcatcatact tatcaatata gttatgcaaa gctaaataat ctacattctc ataagcttct
    15721 tctaatgcga tacgatccca ttcagggtac gatggtaaac gtggcgtaga gcttccaact
    15781 aagattgttt caattgaatc atccactaac ttcatgactt ttgaggcttc atttgctaac
    15841 ctaccatatt cataagcact cttagccccc acttgccatg gaccgtctag ctcattccct
    15901 aaacaccata atttaacgcc aaaaggcttt ttttgcccat tttttattct taaatcactc
    15961 cagaaggttc cttttggaaa attacaatat tctactaaat tttttgcttc atcaattcct
    16021 tttgttccta aattcaccgt gaatatcggt ttcgcttgta catgactgat ccatttaaaa
    16081 aattcgtgga gtccaaaaga atttggttct atacctcgcc atgctaaatc aatttttttt
    16141 ggtcgttgtt ctttcctacc tacagtatcc acccaatcat aaccagatgt aaagtttcct
    16201 ccaggatagc gaatgacacc taattctaat tcctttacat aagagaggac atcttgtcta
    16261 aacccgtcac catcagctgt ttcatggccc ggttcataaa tccctgtata gacgacactt
    16321 cccatatgct caacaaatga gccaaacagc cgagaactga tctctcctac cgtaaaatcc
    16381 tctctgattt ttactttcac ttcttcgtcc aacagctttc cctacctttc atttcctata
    16441 gtcaagataa tagatacaag tttccatacg ccaccagcaa agataaaaat aaaaggaggg
    16501 aaaagaaata tgagcagtac aaatcctaat aacaatacgc aacaaagaat atttctcaaa
    16561 aaatcaaaaa atactttcca tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg
    16621 ttttcagaaa tcacatatat ccaggataaa agaaacatgg aataaataag taaaactaca
    16681 tatccactaa ttgaaagtac gattgtctgt tcttctaata attgattaac aagaaaataa
    16741 ataatgatcg ttaacagact aaggaacaag ttcttaccaa aatgttgctt tattccttga
    16801 aagaaaaaat aaatgatatt tcctgatccc tcacctttcc attgcgccat tacataaaaa
    16861 acagcacatg tagcaggcac gatagtaata acaggcaaac acacgattac ccagagaata
    16921 cctaataaga tgtaatcaga aaccacaatc aactgtccat aaagtttagt ctcaactatt
    16981 ttcatcttcc tcactctttc attccagaca tagctgttcc ttgaacaaaa tatttctgtg
    17041 caaaaatata aaccactagt aatggcagca ttgaaataac cgttccagcc attacaggtc
    17101 cataaaaaga aatgttctgc cctgtaaata aagctaaccc tgctggtaaa gttctcattt
    17161 ttgaactact tgtcaatatc aatggataaa gcaagtcatt ccaactattc atcagcgtga
    17221 aaatccctaa tgataaaaga gtaggtttgc tcaatggcaa catgattttc aaaaaaatat
    17281 taaactcact taatccatca atgcgtgctg cctcttccaa agaatctggt aaagtaataa
    17341 aaaaggaacg catcatgaag ataccaaatg cagttgtcat acgaggaatg atcaaacctg
    17401 cataagtatc aagcatgcca agcaaattga cttcaataaa tagaggaatc atgaagactt
    17461 ggaatgggat catcattgtc agcaaaacga aatagaacaa aactgatttt cctttgaaac
    17521 gcatacgagc aaatgcatac cccgcaagag aatcaaagaa tacagaagtg atcactgtcc
    17581 ctccagcaaa aatcactgta tttttgatgt aatctaataa agggatcgtt gtccaaacct
    17641 taatatagtt atctagggta tagctatctg caaatattct aggcggaaat gagataatgt
    17701 ctttttcgtg tttaaaagaa gaaataatca gccacaaaaa aggaaacgca ataaccaaca
    17761 atataactag catactaatc gttcgaatga tttttttacc actaactgct tttcgactat
    17821 tcacagtaac tgtaatcgag ctttctggtg ccatacaggt ttccctccta tttcatttgt
    17881 ttttctttcc gtgacatgta cacattagtg gagatagcta tagttgcaac aatgataaac
    17941 aaaaccgaag acagtgctga tgcatagccc aattcataag gtgctgtaaa tcctcgactg
    18001 taaatatatt gaacagctgt ttctgtctta aattgtgggc caccagcggt tgcaacatat
    18061 acttggtcaa acacttgcat cgaaccaatc agattcgtta aaatacaaaa acctaaggag
    18121 ggaacgatct gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc accatccatt
    18181 tgagcagatt catacaaaga gggagagatt ccctgaatag cagtcaagag aatcgtcata
    18241 gtaacaccaa aattcttcca aacagtcatg atggctaccg tcggcatagc aagggttgga
    18301 tcgcgaaaaa attgagggat cggcaagccg acttgtgaaa acaagtaagg aatcgcccct
    18361 acattcggat caagcaacat agaaaaaata atcgcaattg acgtcaatga acaaataact
    18421 ggaatgtaaa atgtcgaacg acaaaaacga ttgaatctgg tatttttaga taatgctact
    18481 gctgctaata aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa
    18541 tgaatcaacg aattaactgc tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt
    18601 acaaaatttc ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc aaatgttcct
    18661 aaaagcggta tgacagtaaa taataataaa actaaaaaag atggagttaa aaacaaatac
    18721 gcagcacgat caggacgatt ccaatatttg gtgaatccgt tcatatagtc cacctcatct
    18781 ttcattttga taaaatatac aatcttggtt taaacgaatc tgaataagat atccgaacaa
    18841 tcgctgtgat ttttgcaata acacctatct gcaaatacta ggtcgatcat tcagatatct
    18901 gtatcttgcc aacagtctat cctgtataaa gttctgtact atttttgcag taattgattg
    18961 atggcatcat ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa taatttttca
    19021 atcagtggat tgatgatatc gttattgatc gcagagattt gggtaaaacc tggtaaatat
    19081 ggctctgcat agtccatttg tttcgataat tcagatacaa taggatcatt ttttacttct
    19141 tcatcttttg ctactgaatc caagtaggca ggaaaaccat tttcgatact ccatttttta
    19201 ccaatttcag ttgtattcca atatttcaaa aactcataga cagcttcttt tttattatta
    19261 tctgtacttg ccggaatggc atatcctacg ccatctaaaa tagctgtttt tttgtctgaa
    19321 tcactccctt gtggtaaggt agtcacatta taatcaatct cgtttgcttt caatccgtta
    19381 tttaaccatg gcccattgat ttcaatggct agttgaccag cattcattaa attgtccatt
    19441 tcggcgccag aaatcgattc tggaccagtc ttgtctacat ggatcatttt ttggatcgct
    19501 tttaacgttt taaggttctc aggagacgca aattcagact tagtataatc atcgttcact
    19561 aatttaccgc catttgctaa catccagtta tataacggtg cagttccatc cttattgaag
    19621 acaaaacctg atacattttt actactatca gttaattttg gtgccatttc tgtcaattct
    19681 tcccaagtag taggtggttg ttcagtatct aatccagctt gtttaaacaa atcattattc
    19741 caatacatat acatgccttg tacctgcatg gggacaaaat actgtttacc ttctacttca
    19801 cctaattcaa tagccgtttc tttaaaatca gatttatcta ctccatcata ttcccagaaa
    19861 tcgtctaatg gttgtaccgc tccgttttct acatactgtg caaaatctgg ataatttaat
    19921 gcaataaagt caggtgcttt tttagaagaa atcgctgtag gaagtttttc attcaaattg
    19981 gcccaagtca tgatatccat ttcaattttg atgtttttat catttgtttt attaaattca
    20041 ttaacgattt cttgcaatat ttcaccatca gatgcagtaa acccattcca aaaagtcaaa
    20101 gtcacttctt tatcagaatc agattctgct gaacttccgc caccgcaagc tcctaaaata
    20161 cctgttgcag ctaccaacaa agagccaatt aaccattttt tacctttcat tttttgttcc
    20221 tccttcatct gataaacgac atctgaccac attccaagat agcggtttca ataaacaatt
    20281 gattttccca tcttcagctt ttacatttgt tgaatgactt ggttttactt gttcactttt
    20341 aggaagatta gttgcttttg tatcaaatcc actcatttca gaaaaatcaa tcactccctc
    20401 taatgataat tcagacaagt gaacagttag attcatttct tcttctccac ggttaactgc
    20461 aaatatgaca agttctttgt catttattac ggctatactt tctaaatacg gcacttcttc
    20521 aaaatcttcc gtcgcatagt ttgctgaacg aatttgtggg cttaacacct ctccttgtcc
    20581 atacacagca gcttgcataa atggataata gatcgtctgt ctccaagctt ctccgtttgt
    20641 ttctgtcctg attggtgcaa taacatttac caattgagca agacaagcaa tctttactct
    20701 atctgcatgt ttcaataaag tgattaatag acagcctaat agtaatgcat cttcaaaatt
    20761 atagttatct tctagtaaag gaggagcaat ctgccaaggc gctgcttgct catcttgttc
    20821 cgctgaatga taccaaatat tccattcatc aaaggacagg ttgatctgct tcttactatg
    20881 tttcttcgcc ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc
    20941 taaggaacgt gcaagataat gagaaagatc atttttttgg ttgccataat attgatgcag
    21001 agacaaataa tctatttgat cgtacgcttg atcaagaact gttaactccc aatcaccaaa
    21061 tgtaggcatt tgccgattcg aacttccgca taaaaccacc tcaattgaat catctacgag
    21121 tttcatcgcc ttagccgttt cctctgctaa ccgtccatat tcttccgctg ttttatgtcc
    21181 aatctgccaa ggaccgtcca tttcattccc taaacaccat gtcttaattc caaaagggct
    21241 ctcttgacca ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa aattacaata
    21301 ttcgactaaa ttacgagctg catctactcc acgagtaccc agattgacag ccatattgat
    21361 ttcggccccc acatgctgac accagttata aaattcatga atcccaactt cattcgtttc
    21421 aattgtccgc caagctaaat ccaaccttct aggtctattt gctatgggcc caatactgtc
    21481 ttcccaacga aatcctgaca caaaatttcc accaggatat ctaatcatgg gaatgttcaa
    21541 ttcttttacc aagtgaatga cgtcttggcg aaatccactt ttattagctg atggatgatc
    21601 tggctgataa atcccttcat atactgctcg tcccatatgt tcaataaatg aactgtacaa
    21661 ccgcggatca actttgccta cacgcttgac actactaatc aacattgtgg cttccataac
    21721 tgacctcctt taatttatac atgaataata cacctcgttg aaagcgttgt caataatata
    21781 aatgttaaac ttacttattt tttatcgatt ttaattaacc aaaatattaa taatgattaa
    21841 aacaaataat ttaatataac ttttattaac aaatatgtta atatataaga aaaacctttt
    21901 aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta tatgaagcat
    21961 tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag atgaatgtca
    22021 aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc
    22081 tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc
    22141 gtgtcgataa aatcgatatc agttttccag aaaaaatatt caatgctttt gatacgaaag
    22201 aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca tgcggtttag
    22261 ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg gatcctcgaa
    22321 ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag gcacctaatt
    22381 ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt tcttctgaat
    22441 ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat ggtgtggagc
    22501 taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat acgcctgatt
    22561 ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt accaaacact
    22621 tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc cctaaagagc
    22681 aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg gggggctgta
    22741 ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa gtttactaca
    22801 gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat gaagggacgg
    22861 ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt
    22921 ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc gggaacttct
    22981 tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac ttcatctgca
    23041 atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa tttttccggt
    23101 gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa
    23161 atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc tgcagcatga
    23221 tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt aatcatacgt
    23281 tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat
    23341 acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac atcgggctgg
    23401 ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata aggaagacga
    23461 acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc catcgttagt
    23521 tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag tacaaaagtt
    23581 ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt acgtatttct
    23641 tcttttggat caattgttgg tttgacacct aattcattga taatttctgt ctgcaggctc
    23701 atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc attcgttcca
    23761 tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat ttttgcggat
    23821 tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta tattctttga
    23881 tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata tcttgtaaaa
    23941 ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact ggatggaaca
    24001 tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag tctccttcag
    24061 atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga gtagttactt
    24121 tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca tcttcgatcg
    24181 atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc aattttgtcc
    24241 caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc gttgcttcat
    24301 ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct aattgttccc
    24361 gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct tggaagttga
    24421 ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat
    24481 cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc ataaatgctt
    24541 cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta gctggtatcc
    24601 caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata tatgccgctc
    24661 tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat tccaatagtg
    24721 gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat
    24781 tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc ccttccactt
    24841 ggattaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga actgttgctt
    24901 taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct aatccacgca
    24961 agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact aaccgttcca
    25021 atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa
    25081 cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg atttgatata
    25141 gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg tattactcct
    25201 cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga aaagaaatct
    25261 tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa tgaactccat
    25321 ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat acttatttta
    25381 ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact tgataaaata
    25441 agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac aaatataacc
    25501 aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac gtctttcctt
    25561 ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat ttttttggaa
    25621 aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg
    25681 tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca aaaatggcga
    25741 gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag caggttacca
    25801 taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt tctatcacta
    25861 caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc tgtttcactc
    25921 ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga
    25981 tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt ttatcatatg
    26041 tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa ttaatagccg
    26101 tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt aaattactat
    26161 tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg atttagatcc
    26221 aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa ttgaaccttt
    26281 aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttcg tgagaaaaaa
    26341 atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt tgtcaggagg
    26401 aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca gtccgagaac
    26461 cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt cttcaggtta
    26521 tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt atcgaacaat
    26581 ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg accaccagaa
    26641 agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt
    26701 gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga gtgacaaaga
    26761 aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct atggacaaga
    26821 gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat
    26881 ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat ttgatctcac
    26941 tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc aagctcataa
    27001 gaagtggttc tcttacagct ggcctgaata tcagccaaaa gcgcataaag gactaggaat
    27061 gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg gtcatggtgc
    27121 agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa
    27181 aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta gatttttatg
    27241 ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt taaatatatt
    27301 catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt aagaaacaat
    27361 tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa accaagaagc
    27421 tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg cagatagctt
    27481 tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac tccaagctct
    27541 ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccaa aaaaacaagt
    27601 gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt tgagttataa
    27661 cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg ctgcttcaaa
    27721 taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct tgcctgtttt
    27781 cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa taaatcatct
    27841 agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc aaattcttca
    27901 agataccttc gctctttttc tttatgatag cccgtcggta aaagtgtcac tttgtaatca
    27961 taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg atgattggta
    28021 taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct ggcatttgca
    28081 ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt ttcctcatct
    28141 acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt
    28201 gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga ttttcttgta
    28261 aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct tcatgcgaaa
    28321 aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt tcattcattg
    28381 gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt aatacggatc
    28441 ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt atctctacat
    28501 aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa acgcgtctca
    28561 tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat taccaaagtc
    28621 tcttatttta aattgaatac attataatga tactgtaaat atatctttat tattagtgaa
    28681 t
//
LOCUS       DACCCM010000029        28512 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCCM010000029 DACCCM010000000
VERSION     DACCCM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.030"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(218..1015)
                     /locus_tag="IVK78_001301"
     CDS             complement(218..1015)
                     /locus_tag="IVK78_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP6374781.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IVK78_001302"
     CDS             complement(1012..2196)
                     /locus_tag="IVK78_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6374782.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IVK78_001303"
     CDS             complement(2221..3468)
                     /locus_tag="IVK78_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP6374783.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IVK78_001304"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IVK78_001304"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP6374784.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IVK78_001305"
     CDS             5448..5762
                     /locus_tag="IVK78_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP6374785.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IVK78_001306"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IVK78_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP6374786.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IVK78_001307"
     CDS             6447..7091
                     /locus_tag="IVK78_001307"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP6374787.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IVK78_001308"
     CDS             7106..7435
                     /locus_tag="IVK78_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374788.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IVK78_001309"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IVK78_001309"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP6374789.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IVK78_001310"
     CDS             8423..9247
                     /locus_tag="IVK78_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP6374790.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IVK78_001311"
     CDS             9240..9587
                     /locus_tag="IVK78_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP6374791.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IVK78_001312"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IVK78_001312"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP6374792.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IVK78_001313"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IVK78_001313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP6374793.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVK78_001314"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVK78_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP6374794.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVK78_001315"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVK78_001315"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP6374795.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IVK78_001316"
     CDS             15244..16011
                     /locus_tag="IVK78_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374796.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IVK78_001317"
     CDS             16001..18043
                     /locus_tag="IVK78_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374797.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IVK78_001318"
     CDS             18324..18473
                     /locus_tag="IVK78_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP6374798.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IVK78_001319"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IVK78_001319"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP6374799.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IVK78_001320"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IVK78_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP6374800.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IVK78_001321"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IVK78_001321"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP6374801.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IVK78_001322"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IVK78_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP6374802.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IVK78_001323"
     CDS             22890..23972
                     /locus_tag="IVK78_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP6374803.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IVK78_001324"
     CDS             23972..24775
                     /locus_tag="IVK78_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6374804.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IVK78_001325"
     CDS             24845..25585
                     /locus_tag="IVK78_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6374805.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IVK78_001326"
     CDS             25596..26600
                     /locus_tag="IVK78_001326"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP6374806.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IVK78_001327"
     CDS             complement(26645..28489)
                     /locus_tag="IVK78_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374807.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8992 a         5041 c         5863 g         8616 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac tt
//
LOCUS       DACCCM010000030        28048 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCCM010000030 DACCCM010000000
VERSION     DACCCM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.031"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            226..894
                     /locus_tag="IVK78_001328"
     CDS             226..894
                     /locus_tag="IVK78_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP6374808.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IVK78_001329"
     CDS             complement(1094..1480)
                     /locus_tag="IVK78_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374809.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IVK78_001330"
     CDS             complement(1452..2834)
                     /locus_tag="IVK78_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6374810.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IVK78_001331"
     CDS             complement(2990..4177)
                     /locus_tag="IVK78_001331"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP6374811.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IVK78_001332"
     CDS             complement(4184..5215)
                     /locus_tag="IVK78_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6374812.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IVK78_001333"
     CDS             complement(5231..6685)
                     /locus_tag="IVK78_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6374813.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6860..7765
                     /locus_tag="IVK78_001334"
     CDS             6860..7765
                     /locus_tag="IVK78_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6374814.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            7856..8533
                     /locus_tag="IVK78_001335"
     CDS             7856..8533
                     /locus_tag="IVK78_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6374815.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IVK78_001336"
     CDS             8814..9710
                     /locus_tag="IVK78_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6374816.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IVK78_001337"
     CDS             complement(9765..11135)
                     /locus_tag="IVK78_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6374817.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IVK78_001338"
     CDS             11373..12119
                     /locus_tag="IVK78_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6374818.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IVK78_001339"
     CDS             12116..12955
                     /locus_tag="IVK78_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6374819.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IVK78_001340"
     CDS             12956..13906
                     /locus_tag="IVK78_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6374820.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IVK78_001341"
     CDS             14047..14799
                     /locus_tag="IVK78_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374821.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IVK78_001342"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IVK78_001342"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP6374822.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16354..16962
                     /locus_tag="IVK78_001343"
     CDS             16354..16962
                     /locus_tag="IVK78_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6374823.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            17130..18767
                     /locus_tag="IVK78_001344"
     CDS             17130..18767
                     /locus_tag="IVK78_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP6374824.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(18839..19951)
                     /locus_tag="IVK78_001345"
     CDS             complement(18839..19951)
                     /locus_tag="IVK78_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP6374825.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(20026..21399)
                     /locus_tag="IVK78_001346"
     CDS             complement(20026..21399)
                     /locus_tag="IVK78_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6374826.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            21620..22237
                     /locus_tag="IVK78_001347"
     CDS             21620..22237
                     /locus_tag="IVK78_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374827.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            22335..22790
                     /gene="argR"
                     /locus_tag="IVK78_001348"
     CDS             22335..22790
                     /gene="argR"
                     /locus_tag="IVK78_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6374828.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            22808..24310
                     /locus_tag="IVK78_001349"
     CDS             22808..24310
                     /locus_tag="IVK78_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP6374829.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            24405..24611
                     /locus_tag="IVK78_001350"
     CDS             24405..24611
                     /locus_tag="IVK78_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374830.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            24697..25098
                     /locus_tag="IVK78_001351"
     CDS             24697..25098
                     /locus_tag="IVK78_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6374831.1"
                     /translation="MKKAYETAMINHGGRNGEVEEPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVFEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            25253..26584
                     /locus_tag="IVK78_001352"
     CDS             25253..26584
                     /locus_tag="IVK78_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP6374832.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            26606..27232
                     /locus_tag="IVK78_001353"
     CDS             26606..27232
                     /locus_tag="IVK78_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6374833.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            27415..27996
                     /locus_tag="IVK78_001354"
     CDS             27415..27996
                     /locus_tag="IVK78_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6374834.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
BASE COUNT         9351 a         5150 c         5740 g         7807 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggatctactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacaaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccacagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagaat gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa aacatagaaa aacgattact ttgcttatac aacaaggctt ttatggtttt
    16201 gtatcaaaaa aatatttgtt taaatgttac aaatattaaa taattcgtta ttttgtcatt
    16261 aaatgtcatg tttcttttac ctatgaaata tttctaaatg ataaagttaa tagcgtcgaa
    16321 agaataaaaa acggagaaaa aaaggagttt aatatgaatt catttaagaa aattgtttta
    16381 ggtacaactt ttgctgctgg tgctactgca atgtttgtag gaactacaaa tgctcacgct
    16441 gatgaagttt atacagtaaa atcaggtgat tcattatcta agatctcaca aaaatttgca
    16501 ggagacaact caatgatcga tgcaattgct gaaaaaaatt cgatcgcaaa catcaaccgc
    16561 atttatgttg gtgaacaatt aacaatccca acttcaaatg attcttcagc aacaacagaa
    16621 aacaaaacaa cagaaaatac agcatctaca actgaaacag caacgcaaga acatacatac
    16681 gttgcacctg ttgaaacagt tgaagtagct cctgctgccc cagctgctgc aacagcgcca
    16741 acttcaagct ctgcaaaaga atggatcgca caaaaagaat caggcggttc atacacagct
    16801 actaacggac gttacatcgg ccgttatcaa ttagatgctt catacttaaa cggcgactat
    16861 tcagctgcca atcaagaacg tgtagcagaa caatatgtaa catctcgtta tggttcatgg
    16921 gatgctgcta aaacattctg gttagcaaac ggctggtact aagccgataa aaggaaaatg
    16981 atgaaaccga gaaacttgtt tattcaagtt ctcggttttt ttgtgtgaaa aataactcgt
    17041 ttgcttccca cagcgcagca gtacaaataa aaactcgaat aatgtataat aaagaaaacg
    17101 tttttttgtt tagggaatga ggaggagcca tgagtacaaa agagagtctc gaattaaacg
    17161 aaatcacaag tggaaatcca ctgttatcca gtattcgatc gattgtagaa acgacatttt
    17221 acggtaataa tgttcatgaa gtttttgata ggaagacagc ttatcaacta gcaaaaggga
    17281 gtccaggaac gatcatcaca gatttaacaa tctcacatgc tgaagaacta gatttaccag
    17341 ctgatgttag gacactagta ttcaatgatg gaagcattgt gggaagaaca gcaagtgctc
    17401 ggcggatctt tgaggactta gacaaggaac aaagcaaata cgagaaaatt ttaagagaag
    17461 ctgtgtatca aagtcgcaag cgccagtttt accatacaaa agtaatcgtt ggcctatctg
    17521 aagaattttc tgtgcaaagt catttgctga ttccagaagg atttgagaac aatttgtatt
    17581 cttatttgct gaattttcag ccagcgaatt cgaagatctt agagacttat caaaagtctg
    17641 tttcctttga tgagggagat ctttatatct tttgtgatcc cgaatggaca catgaaacat
    17701 ttccaaatgg actcattttg tttgatgctc tccataatac agcggttgtg ctaggattac
    17761 gttactttgg agaattaaaa aaagcgactt tgacgcttgc ttgggcattg ggaaatcgca
    17821 atggctttat tgcctgccac ggtgggatga aacaatatgc tagtaatgac cagacctata
    17881 cgatggctgt atttgggttg tccggttctg gaaaatcaac gattacctta gcaaaacata
    17941 cggaaggtca ctctgtttcg atcttgcatg acgatgcttt cgttatttct cgaaaaaatg
    18001 gttttgctac tgccttggaa ccttcttact ttgataaaac acaagactat ccgtctgatg
    18061 atccggccat ttcttctttc cttacttgtc agaatgtggg cgtgacatgt acaagtaaag
    18121 gagaaaaagt cctagtaaca ggagatatac gaaacggaaa tgggcggaca gtcaaatcac
    18181 gttatgcgac acccgaccga gtcgatcatc ttagcgaaaa gatcgatgct gttttttgga
    18241 tcatgaaaga tgattcgctt ccgccaatta tcaagatcaa tgacccagtt ttagcagcgt
    18301 cttttggtgc aacattagca acaaaacgat cagacgcgga aaatatcact acaaaaagac
    18361 agctagatga gctagtcatt gaaccatttg ctaatccttt ccgtgtctat cctcttaggg
    18421 aagactatca agcttttcga gagttgtttt tgaaacagca aacagcctgc tatgtcctga
    18481 atactggata tttcgatgat aaaaaaatca aacctgaaac gacattgtca atcgttgaaa
    18541 gaatcgtgca acaaacggca gaattcaagt cgtttggtcc atttgaagac atccactatt
    18601 tacctatacc agattttcct gtttcttttg aggatacgtc ctataaaaat ctggtgaaac
    18661 aacgacttga tattcgttta cgattcattg aagctcagat aggaaaagat ctcttgccag
    18721 atgaagcaaa agaaagtttg gagaatttga ttcagaaatt gaactaaaat aacagctttt
    18781 tcaagaagta atccatgcgc aatatcgctc gggatcctta cttgaaaaag ctgtttttct
    18841 atgatggatt tttcgtaata taggtggaga tgtccttttg ataatcttcc gtaggccaat
    18901 gaccagtatt taagaagttg gctaatagat agccgatata aggacctgtg gtcaagccag
    18961 aagagcctag accgctagca gccagtaagc ttggatcatc tggcagtacg ccgaaaaatg
    19021 gggaaaaatc tgacgtataa gcacgggtac ctacacggaa ctcgtatggc cagtttttta
    19081 tctgatctgg atcagtcaag aactgttctg tactcttagt caatccctta taagcttcgc
    19141 tagtaggagc caaatcccag ccttgatcgt tttcatgggt agcacctaaa aggatctttc
    19201 catccatgaa aggaatgaaa tcagcttctc cgtcaagcat agcaacaggc cagtcttgac
    19261 tcatcttata agatgtctga aatgctaaga gctgcccttt ttgcggacgt atatctgcag
    19321 catagcctaa ttcttgtaat aaaggcttaa gtgccgggcc aggtgtcaaa attacacgat
    19381 cagctgtgta ttctccaaac ggtcccttga tgagccaaga atcgctcttc ttatgcaaag
    19441 tgacttttgc ctggatctgc tccatttgtt tttgtgctaa acgctgacca agatgttcta
    19501 aatatgcttt tccatccaga cgaccaccac cactgatacg cagtgaaact gctggtttca
    19561 ataacggaag tgcttgagat gtctgttcag gtgaaagcca ttcgatgttt ccaatttctg
    19621 gcgcttcttc ttttcgtttc tcggcaagaa cagccaattc agctaaatct ttttcaggtc
    19681 gaagcaaaag tgtccctgat tgtgcgtaaa tcgtttgatc aagttggaaa tcttttataa
    19741 gtttagggaa aaaagcagcg ccgtctttag ccagacgata ccattgtttg tttctgcgtt
    19801 tcgacaacca aggggagata atcccagcgc tagctttagt tgcctgtcct gtcccttcat
    19861 caaataaagc aacattgaat tttgatgtat ctaagtaact ggctagggtc ataccaatga
    19921 tcccgccacc gataatggca attttttcca taaaaaagcc tccttttaca aacaaccatt
    19981 ttcacacaaa aaaacggcag atgcaacacc tgccgtatat ggaatctacg cttttttagc
    20041 aaagaacaga taataggcga atcgagcaaa gcttacgaga agcaacagca acaagacgcc
    20101 taatgctgct tttgatccta atgtagtaga cacttcatag ttatgggaag tatgatgctg
    20161 gacgtaattg ataatgaccg ctactaatga agtagccaca gcgccagaaa actgctgcaa
    20221 ggtattgaac attgcgttcc cgtctccttg tagttcttca ttcagtgcat tcattccggt
    20281 ggtcatcata ttgctgtaag ataatccgat accgatcata taaaacacat gacaagcaat
    20341 caatacaccc actgaagcgt gtcctatgag taggaataat gctagccaac caatcaaaga
    20401 aagaatcagc ccagcaaaaa ttggcttctt cggaccatat tgatccagca ttttcccgga
    20461 aaaaggagaa agcaaagcac caaccgctgc gccgggaagc atcgctaatc cagcgacaaa
    20521 aggactactt ccttgaacga tctgaaggaa attcggcaag acaaaagata caccaagtaa
    20581 taaaaactga taaactaaga agccaatcaa aaacaagcgg aatgttttat ttgaaagaat
    20641 agacagtcgg atcagtggat gctccacttt tttacttcga tagtaaaaaa ggaaccagcc
    20701 aataccgccg atgattaaag caatccagct ggaaactgtt cccaacgtac tcaaaaatgt
    20761 tagacttcca ctaaacaaaa gacaaattcc taaaacgctt atccaatcca aataaccact
    20821 tttttgtact gccatctcgg gaatagcaaa ccagccgata atcaaagaaa gaatcaaaac
    20881 gggcacaaga aacaggaaga tatgattcca tgtaaaatgt gaagtcataa tgcctccata
    20941 agtggggcca attgctggag caatggccgt tgtcaaagta ccgacaccca tcatcgcgcc
    21001 tcgtcgattg aggggggcaa atgtcaagat aatatggaac atcaacggaa gagcgatacc
    21061 tgtcgctgca ccttggagca atcgaccaaa caataggacc atgaaggtag gagagaagaa
    21121 atcgatcatt atacctgcca agaaaagcag atttgaagct aaaaagagtt ttttgagact
    21181 aaaattgcga ttcaaataag tagacagcgg aacaatgatc gaaatcatca aaagataaat
    21241 cgttgttacc cattggactt gtgcagtcgt taaatcaaac tgtctgataa gagttgggaa
    21301 agttacattc atggccgtct cgatcaatac gcccgcaaaa gacatcatcc ctgttgcaat
    21361 tactgcaggt actaaaagca gctgtttttg ttttgtcatt tacatcacct ctaaaaaatt
    21421 tcgtaaaaaa agagagtgat ttctattata aaaatagaaa ccgcctctct ctcgacacgc
    21481 cgaaacgtgt tatctgtacg atttatttac ttcacttatt ctagcacaaa ctgaaaaata
    21541 gtgtcaacac gattagccaa gtcactcaaa aagaggtatg ataataaata actaaatgga
    21601 atttcgcgga ggaagaaaaa tggaaaaaat tgaatggcgt aaagacaaga gctatagtat
    21661 gaaaaaaagc ccgcaaatac tagaggttcc taaaaaatca ttctttagca tcagagggat
    21721 cggcgacccg aatcaggaag actttaaacg aagagtagcc tgtctatatg cagtcagcta
    21781 tgcaatacgc atgtcgccta aaaaaaattg gctgattcct gattatcaga tgtatactgt
    21841 ctatccactg gaaggacaat ggggactgca agaaaagtat ttgcatgaac cagtgatgaa
    21901 aaaagaacat ttttcttatc aattgatgat ccgacagcct gactttgtta cagaagcgat
    21961 agcacaagaa gccattcagc gttctagcaa acttccggaa gacttgaaag aacaagtagt
    22021 attcaaaaag atggaagaag gtctttgtgc ccaaatctta cacgtcgggc cctatgccga
    22081 agaaccagca acgtttgaga aattagaagc attcatcgca aaagaaggct atgaaagagc
    22141 ttctaaagaa cacaaagaga tttacatcag cgatccaaga aaatcagcac ctgaaaaaat
    22201 gaagacgatt ttgcgtgtta aaatagaaaa aggttagcaa cctgaatgaa aacaaaaaat
    22261 aaaagtaaaa aataccaaaa tatgaagaaa tattacttat cttttgctat aatcaacatt
    22321 gagaggtgtc taatatgaaa aagaaacagc gacaagcact gatccgccag atcattacag
    22381 aacaacctat tgggacacag gaagagctgc ttgcgcggct gcatgaagca ggcgccgatg
    22441 tgactcaagc aacgatttct cgagacattc gagaaatgaa gttagtaaaa agtcaaaatg
    22501 aaaacaaaat agtacgctat acattattta accagccatc tgtttcttta aatgaagaac
    22561 gtttgcgtac agcaattaga cgcgaggttt tgcgtatcca gtctgttcag ttcatggtta
    22621 tcgtattgac tgaacgaaat ggtgccgatg tggtaacgaa ttggctagat gaagtggctt
    22681 accctgaagt tgtcggaacg atggccggtg tagatacatt cattattatt tgccgctcag
    22741 aagaagaagc acagcgattt gcagaaaagc tggaaaaaat gagggaataa atgaggagga
    22801 cttttatatg aaagatatga aagcgctgca aaatggatcg gatattcgag gaatcgcatt
    22861 accagttgaa ggatatgaag taaacttgac gacagacatg gtaaaaaaga tcggctgggg
    22921 actagcaaac tggataaaga aaaagaagaa atcaaccgat ccaaataaaa aatggacgat
    22981 tgggattggt catgacagta ggataagcgg accagcactg aaagaggcgt tgatccaaag
    23041 tctgaaagaa caagggatac atgtcatcga tttcgaatta gctactacgc ctgctatgtt
    23101 tatgtccact caatttgaac agttctcgtg cgatgctgcg gttatgctga ctgctagcca
    23161 tcttccctat tattataatg gaataaaatt ctttacctca gaaggaggag cagaaaaaga
    23221 agatattgct tttatcctat cacagcagca aaaaataaaa agcgaacaaa ctggaagcgt
    23281 agaaaaagca gaccttttga caccttatgc gcaagattta gtcacaaaaa ttaaaaaggg
    23341 gattggaaca ctagaaagca aaccactaaa tggttggaaa atcattgttg atgccggaaa
    23401 tggtgctggt ggattctttg ctgaaaaagt attgaatagc ttaggtgcgg attcaacagg
    23461 ttcccaattt ttggaaccag atggccgatt tcctaatcat atcccaaatc ctgataataa
    23521 agaagcgatg gaaagtatca agcaggctgt tttagcaaat gaagcagatc ttgggatcat
    23581 tttcgataca gatgtggatc gttctgcagt tgtcagtgct tctggaacag tgatcaatcg
    23641 aaataatttg atcgcagtat taagtacaat cgtattaaaa gagcatccag gaacaagaat
    23701 cgtgacgaat tcccctactt cttctcatct acaaagattc attgaagaaa aaggaggcat
    23761 ccaagttcgt tatatttccg gctatcgaaa tgtaatcaat aaagcattag aacttaaccg
    23821 tcttgggaaa gatacacagc tagccatcga aacaagcgga cacgctgcat tcaaggaaaa
    23881 ctattttctg gatgatggtg catatgtcat cgcaaagatc ctgatgcttt tgccagaatt
    23941 aaaaagaaaa ggtatcacat tagatgaaca aatcaaagag ttgaaacagc ccgtagaagt
    24001 acaagaaatc cgattaaaat tgttcagcga tgaaccacgc gaacaaggaa acaagatatt
    24061 agaagaaatg aagcgactgt tggaagaaga aaaagggatg aacttcgacc cggaaaatga
    24121 agaaggtatt cgatatgatc tagaagagcc atacggaaac ggctggtttt tgatccgttt
    24181 aagcttacat gagccgttac tagtattaca gatagaaaat gatgaattcg gaaagaacaa
    24241 gttggcgtta gtaaaattag caaaattatt cggacatttt tctgatcttg aatcattaac
    24301 agcagaataa accagtcttt ttgaaaggct agttgcttgg acggcaaaaa aagtggtatg
    24361 gttaaatcga gtgctggtag ggagaccagc aaaaggagga aaaaatggta acgaatatgg
    24421 ttattttttt aacagtattg gtttatgggg tcaactttct ggtatatatg tatcttaaaa
    24481 tcaatcatat aaaagattat gtagaaaaga ttgctattta ttttggaaca aatatgacgc
    24541 tgttgttcac gagtagtatt tttttgtttt ttggaaaaat agtagaagac ggcattctgg
    24601 tattcgaata agcagaattt ttgtagaaag cgccttactt gtcatacact ataattatag
    24661 ccaagtagca aatggtcaat cgaaaggagc agtacaatga aaaaagcata cgaaacagca
    24721 atgatcaacc atggcgggcg caatggagaa gtcgaggaac caaatggctc gatgcatatg
    24781 aaaatcactc ctccaggaat ccatgcagaa ggaacaaatc cagaacaatt atttgcagca
    24841 ggttatgctt cttgtttcaa tggggcggta caacatatga tcaaagaagc aggaatgaca
    24901 gcagagtcca cagtaaaagc acgtgtctct ctttataatt tagatgatgg cggctatcag
    24961 atcggtgtag tattcgaagt gcatatcgat ggcatcagcg aagaagaagc ccaaaaaatt
    25021 gcagaagatg cgcatgaatt ctgcccttac tccaaagcaa ctagaggaaa tatcgatgta
    25081 gaagtaacga ttgtttagaa gaataaagga aatcgaacca gactaaaaac gtgaaaggac
    25141 cgctttttgt ctggtttttt ttgacaaata aagcagatga tttggagaat agacaattat
    25201 cggttatcat atttttgtac caatcggtag gtaaaaaaga agcgagggaa aaatgatgga
    25261 aaaatatcaa aatattgtga tcggttttgg aaagggcggt aagactttag cgaaggcgtt
    25321 agcttcaaaa ggtgaatcgg ttttagtcgt tgaaaagtca acacgtatgt atggcggaac
    25381 atgtatcaac atcggctgta ttccttctaa atcattgatt ttcaatggag aaagaggaat
    25441 cgactttaca gaggctgttg ctcggaaaga aaaattgacg ggcatgcttc gcgccaaaaa
    25501 ttatcatatg atcagcgatg aggcaactgg gacagtgatg gacggtacag cacgttttct
    25561 ttctaaccat caaatcgaag tgacgaataa tggtgaaaaa gtgattgttg aaggcgaacg
    25621 gattttcatc aatactggtt ctgaaccaat cattttacca atcaaaggat tgaatacaag
    25681 tcgctacttg attgattcta cgcaagcgat ggatcaggaa caattaccgg aaaaattagt
    25741 gatcatcggg gctggatata ttggcttgga atttgcatca atgttcaatg aatacggatc
    25801 taaagttgtc gttcttgatg cccattctga atttcttcca cgtgaagatg aagatatcgc
    25861 gcaaatgatt ttagaagaca tgacaaatgc gggaatcgaa ttccatctag gtgtatctgt
    25921 agatcaagta gcagaccaag aatcaacagc tgccgtcacc tttacagaag atggccaaga
    25981 agtaacaatc caagcgtcaa aagttttggc tgcaactgga agaaaaccaa gtacagcatc
    26041 acttggatta gaaaatacag atgttaaact aacagatcgc ggagcaatcg ctgtggatga
    26101 ataccttcgt acagatgcag acaacatttg ggcgattgga gacgtcaaag gcggattgca
    26161 gttcacttat atctctctag atgattatcg aatcattatg gatcagttga agggcgagaa
    26221 taaacgaaca acgaataacc ggaaagctgt gccttacagt gtatttatca ctccgacttt
    26281 atccaatgta ggtttaacag aaaaacaagc caaagcacaa ggcgtcaatt acaaattatt
    26341 caagtatatg acaagcggtg tccctaaagc acaagtatta gaagatccta aaggtgtatt
    26401 caaagtttta gtcgaccctg aaactgactt gattctaggg gcaagtatct atgcggaaga
    26461 ttctcatgaa gtcatcaact tgatttcgct agctatgaat ggaaaacttc cttatacgtt
    26521 attgagagat caaatctatt ctcatccgac aatgagtgaa gctctaaatg atgtattaaa
    26581 ataaataacg aatggagaat ccaccatgtt caataataaa aatacagcag tagttctcac
    26641 tgacccgcaa gtcgagttct tgaaaccagc aggcggtggc tttggtttga caaaagatat
    26701 cttagaaaaa tacaatacga tcgaaaatat gattgaaatg gtaaaacaag caaaagaaaa
    26761 aggatacaaa ctctttattt ctcctcatta tttttaccca catgaccata aatggcaatt
    26821 tggcggtgca ggagaacata tgatgcttga gcagcagatg tttgctagaa aaagtcaata
    26881 tgaatcagtg gaagaaggtt caggagccga ttttatcgaa gaattgaaac cttatttgga
    26941 tgaaaatacg atcatcagct ctcctcataa aatctttgga ccagaaagca atgatttggc
    27001 gctccaattg agaaaaaatg gaatcgacac agtaattctt ggtggaatga acggaaactt
    27061 gtgtagcgat tcccatatgc gtgaattggt agagagtgga ttcaatgttg tggtagcgac
    27121 agatgcaatt ggtgctcctg gccaagaagc aatggatgca gcgttgacga actttggctt
    27181 tattgcagat caagtgatga caacagagga agtattagag caactatcat aagataggaa
    27241 ttgcttttgt cctcgcagtt cttgaatcaa tgaacatcta gctatatgag aacagtttta
    27301 tagtgagggc tgtgtcagat aatttggcac agtctttttt aaacgattaa gaagaaaaaa
    27361 tgatgaattt actgtataat ataggactat gagaaatgaa aaaggagaat atcattgaaa
    27421 acaaaggaaa agatcttgga agcgaccaaa gatcttattt ataaaaaagg ctatcatgaa
    27481 acgtcaatcc gagatattct agcagcttct gatacaggaa aaggtcaatt ttattattac
    27541 ttttcttcaa aaaaagaagt ttgtctagcg gtgatccatg cgcatatgga cgtatggcag
    27601 acagaattgt tcgaagacat cttaaatgct ggaaaacagc cagaagaagc attagaagaa
    27661 atgttggact ggattttcgc tgatcatgag acacagatca cttatcatgg ctgtcctgta
    27721 ggtaatttga ttattgagtt gagtacagtg gatgaagaat accgtctgtt gctactagaa
    27781 ttagtcaatc aatgggtcaa gtatacgaaa caaaatatcc tggcactttc tgaagaaaaa
    27841 ctgtccgatg aagaagcaga ccgtcaagcc aaagcgttga ttgctgaaat ccagggaagt
    27901 atcttattgt tgaaagtcac acaggatcta tcactactta ggcagaattt tcaattggtc
    27961 aaagaaagat tgttaacttc gcaaaaggtt ctttaataat tagaggttgt gacagaagtg
    28021 gtcagcttca agaaataaga aggaattt
//
LOCUS       DACCCM010000031        27633 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCCM010000031 DACCCM010000000
VERSION     DACCCM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.032"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            119..766
                     /locus_tag="IVK78_001355"
     CDS             119..766
                     /locus_tag="IVK78_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP6374835.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            850..1794
                     /locus_tag="IVK78_001356"
     CDS             850..1794
                     /locus_tag="IVK78_001356"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP6374836.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1929..3380
                     /locus_tag="IVK78_001357"
     CDS             1929..3380
                     /locus_tag="IVK78_001357"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP6374837.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3401..4237
                     /locus_tag="IVK78_001358"
     CDS             3401..4237
                     /locus_tag="IVK78_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6374838.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4237..4896
                     /locus_tag="IVK78_001359"
     CDS             4237..4896
                     /locus_tag="IVK78_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6374839.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5022..5711
                     /locus_tag="IVK78_001360"
     CDS             5022..5711
                     /locus_tag="IVK78_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374840.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5717..6673
                     /locus_tag="IVK78_001361"
     CDS             5717..6673
                     /locus_tag="IVK78_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP6374841.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6717..7229
                     /locus_tag="IVK78_001362"
     CDS             6717..7229
                     /locus_tag="IVK78_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6374842.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7485..8033)
                     /locus_tag="IVK78_001363"
     CDS             complement(7485..8033)
                     /locus_tag="IVK78_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6374843.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8122..9303)
                     /locus_tag="IVK78_001364"
     CDS             complement(8122..9303)
                     /locus_tag="IVK78_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP6374844.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9539..11047)
                     /locus_tag="IVK78_001365"
     CDS             complement(9539..11047)
                     /locus_tag="IVK78_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP6374845.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11206..11397
                     /locus_tag="IVK78_001366"
     CDS             11206..11397
                     /locus_tag="IVK78_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374846.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11600..12517
                     /locus_tag="IVK78_001367"
     CDS             11600..12517
                     /locus_tag="IVK78_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP6374847.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12510..13583
                     /locus_tag="IVK78_001368"
     CDS             12510..13583
                     /locus_tag="IVK78_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374848.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13731..14249
                     /locus_tag="IVK78_001369"
     CDS             13731..14249
                     /locus_tag="IVK78_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374849.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14230..14622
                     /locus_tag="IVK78_001370"
     CDS             14230..14622
                     /locus_tag="IVK78_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP6374850.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14698..15633
                     /locus_tag="IVK78_001371"
     CDS             14698..15633
                     /locus_tag="IVK78_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP6374851.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15766..16545
                     /locus_tag="IVK78_001372"
     CDS             15766..16545
                     /locus_tag="IVK78_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP6374852.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16538..16855
                     /locus_tag="IVK78_001373"
     CDS             16538..16855
                     /locus_tag="IVK78_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6374853.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17099..18709
                     /locus_tag="IVK78_001374"
     CDS             17099..18709
                     /locus_tag="IVK78_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6374854.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18866..19714
                     /locus_tag="IVK78_001375"
     CDS             18866..19714
                     /locus_tag="IVK78_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6374855.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19945..20424
                     /locus_tag="IVK78_001376"
     CDS             19945..20424
                     /locus_tag="IVK78_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374856.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20457..22685
                     /locus_tag="IVK78_001377"
     CDS             20457..22685
                     /locus_tag="IVK78_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6374857.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22814..24457
                     /locus_tag="IVK78_001378"
     CDS             22814..24457
                     /locus_tag="IVK78_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6374858.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKINK"
     gene            complement(24530..25075)
                     /locus_tag="IVK78_001379"
     CDS             complement(24530..25075)
                     /locus_tag="IVK78_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP6374859.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25098..25583)
                     /locus_tag="IVK78_001380"
     CDS             complement(25098..25583)
                     /locus_tag="IVK78_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6374860.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25573..26385)
                     /locus_tag="IVK78_001381"
     CDS             complement(25573..26385)
                     /locus_tag="IVK78_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP6374861.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26524..>27633)
                     /locus_tag="IVK78_001382"
     CDS             complement(26524..>27633)
                     /locus_tag="IVK78_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6374862.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9048 a         5026 c         5526 g         8033 t
ORIGIN      
        1 tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa
       61 ttttttcttc gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat
      121 gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc
      181 aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca
      241 ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag
      301 tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca
      361 agataaatac ccagatactg caattactag tttggaatta gatagtgatt ggggatctta
      421 cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca
      481 aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca
      541 aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa
      601 aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt
      661 aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat
      721 caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa
      781 aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga
      841 gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg
      901 aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa
      961 gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca
     1021 cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt
     1081 tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg
     1141 ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata
     1201 gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga
     1261 aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc
     1321 atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat
     1381 cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg
     1441 ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg
     1501 ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt
     1561 tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa
     1621 gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac cgagcttcat
     1681 aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa
     1741 aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga
     1801 gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct
     1861 gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg
     1921 aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt
     1981 ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg
     2041 cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag
     2101 aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg
     2161 gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta
     2221 attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag
     2281 agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga
     2341 accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa
     2401 tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt
     2461 gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga
     2521 gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg
     2581 ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg
     2641 agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga
     2701 tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg
     2761 gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca
     2821 cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag
     2881 gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat
     2941 atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag
     3001 caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat
     3061 ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac
     3121 acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga
     3181 gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct
     3241 tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag
     3301 gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt
     3361 taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt
     3421 tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca
     3481 ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg
     3541 cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga
     3601 tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt
     3661 ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg
     3721 tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga
     3781 cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg
     3841 gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc
     3901 taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg
     3961 tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc
     4021 agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt
     4081 attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa
     4141 agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa
     4201 accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct aattgatttt
     4261 tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt
     4321 ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt tttaggtctt
     4381 ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt
     4441 cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt aagtgcctta
     4501 acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga
     4561 ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct
     4621 ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca
     4681 ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg
     4741 ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc
     4801 tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag
     4861 tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg
     4921 ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc
     4981 atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca
     5041 tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag
     5101 aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg
     5161 atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta
     5221 cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg
     5281 aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg
     5341 ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag
     5401 agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag
     5461 taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat caattgtaca
     5521 aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac
     5581 ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc
     5641 ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc
     5701 cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc
     5761 attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc
     5821 agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg
     5881 aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca
     5941 cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa
     6001 tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc
     6061 aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga
     6121 tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca
     6181 aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta
     6241 tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg
     6301 ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc
     6361 ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca
     6421 agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca
     6481 tcaactcttg attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt
     6541 ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca
     6601 cctgctagat catccaaaag attggcttaa atattattca aaagttcagg aactatatga
     6661 agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt
     6721 atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag
     6781 cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag
     6841 aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa
     6901 ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa atagatggta
     6961 gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta
     7021 ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca
     7081 aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca
     7141 tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa
     7201 gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca
     7261 ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag
     7321 cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg
     7381 tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt
     7441 tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat caataatcct
     7501 ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag
     7561 gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct
     7621 gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag
     7681 atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca
     7741 tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta
     7801 ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc
     7861 atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt
     7921 cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg
     7981 agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt
     8041 tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa
     8101 gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac ttaaaaagct
     8161 gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc
     8221 ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga
     8281 aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc
     8341 taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc
     8401 atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc
     8461 taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat
     8521 caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag
     8581 aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc
     8641 atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa
     8701 taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc
     8761 gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc
     8821 agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc
     8881 agaagctact ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag
     8941 accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc
     9001 agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc
     9061 tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat
     9121 ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag
     9181 aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact
     9241 gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact
     9301 caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact
     9361 aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa
     9421 aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa
     9481 aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct
     9541 attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt
     9601 ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct
     9661 caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg
     9721 ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg
     9781 atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga
     9841 tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac
     9901 ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat
     9961 cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt
    10021 ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt
    10081 cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt
    10141 cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca
    10201 cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt tcaataatac
    10261 gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg
    10321 catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat
    10381 aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg
    10441 agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag gaagtagcga
    10501 gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat
    10561 actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt
    10621 cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt
    10681 tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac
    10741 cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac
    10801 ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt
    10861 tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg
    10921 catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca tagtggttca
    10981 ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt
    11041 ttttcactag gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc
    11101 aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag
    11161 atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag
    11221 aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg
    11281 cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta
    11341 actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa
    11401 ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga
    11461 aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt
    11521 tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga
    11581 gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga
    11641 actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat
    11701 caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa
    11761 agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga
    11821 agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat
    11881 ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac
    11941 gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc
    12001 aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca
    12061 accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc
    12121 tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga
    12181 tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt
    12241 agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac
    12301 gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct
    12361 tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga
    12421 gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa
    12481 taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa
    12541 aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa
    12601 gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa
    12661 aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa
    12721 ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga
    12781 ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag
    12841 attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca
    12901 cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg
    12961 atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga
    13021 tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg
    13081 atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa
    13141 tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat
    13201 tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa
    13261 gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga
    13321 atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa
    13381 tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag
    13441 ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct
    13501 catctggaga ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc
    13561 cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat
    13621 tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg
    13681 tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa
    13741 ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc
    13801 aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa
    13861 gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg
    13921 gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt
    13981 caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt
    14041 ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt
    14101 tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag
    14161 gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag
    14221 gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt
    14281 ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa
    14341 acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa
    14401 gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa
    14461 gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc
    14521 tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg
    14581 aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt
    14641 aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg
    14701 gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta
    14761 gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa
    14821 agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa
    14881 gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc
    14941 agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct
    15001 tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata
    15061 ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa
    15121 gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa
    15181 atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca
    15241 ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg
    15301 atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc
    15361 atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct
    15421 gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt
    15481 gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat
    15541 attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca
    15601 gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa
    15661 atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta
    15721 caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa
    15781 agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca
    15841 gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga
    15901 ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg
    15961 acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt acgctataca
    16021 ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc
    16081 ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac
    16141 ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc
    16201 ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc
    16261 cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta
    16321 ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa
    16381 aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag
    16441 aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag
    16501 ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact
    16561 tttagttgtt tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt
    16621 tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt
    16681 atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg
    16741 gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat
    16801 ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt
    16861 tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg
    16921 catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt
    16981 ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga
    17041 ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat
    17101 gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt
    17161 tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat
    17221 gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg
    17281 tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga
    17341 taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat
    17401 cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc
    17461 taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc
    17521 aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac
    17581 ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt
    17641 gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt
    17701 cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt
    17761 cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc
    17821 tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt
    17881 tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc
    17941 agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt
    18001 gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat
    18061 gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt
    18121 attgattctt cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt
    18181 gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt
    18241 gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt
    18301 tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt
    18361 aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt
    18421 tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag
    18481 caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt
    18541 tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg
    18601 tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg
    18661 gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc
    18721 gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt
    18781 ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat
    18841 tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata
    18901 tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat
    18961 ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt
    19021 aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt
    19081 atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg
    19141 gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt
    19201 cattccttat gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt
    19261 agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg
    19321 agataatacc tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg
    19381 tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt
    19441 ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg
    19501 agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt
    19561 gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata
    19621 tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga
    19681 aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa
    19741 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
    19801 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
    19861 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
    19921 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
    19981 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatac ggatgcaaaa
    20041 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
    20101 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
    20161 attttagatt tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt
    20221 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
    20281 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
    20341 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
    20401 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
    20461 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
    20521 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
    20581 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
    20641 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
    20701 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
    20761 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
    20821 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
    20881 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
    20941 attacccttc tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc
    21001 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
    21061 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
    21121 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
    21181 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
    21241 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
    21301 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
    21361 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
    21421 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
    21481 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
    21541 agcatacaaa aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
    21601 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
    21661 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
    21721 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
    21781 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
    21841 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
    21901 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
    21961 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
    22021 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
    22081 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
    22141 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
    22201 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
    22261 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
    22321 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
    22381 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
    22441 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
    22501 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
    22561 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
    22621 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
    22681 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
    22741 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
    22801 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
    22861 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
    22921 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
    22981 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
    23041 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
    23101 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
    23161 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
    23221 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
    23281 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
    23341 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
    23401 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
    23461 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
    23521 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
    23581 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
    23641 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact
    23701 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
    23761 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
    23821 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
    23881 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
    23941 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
    24001 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
    24061 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
    24121 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
    24181 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
    24241 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
    24301 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
    24361 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
    24421 accttatgaa gctcggttat ataaaataaa caagtaaaac ttcctcgtta aagttcatta
    24481 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
    24541 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
    24601 gggttttcaa acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg
    24661 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
    24721 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
    24781 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
    24841 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
    24901 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
    24961 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
    25021 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
    25081 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
    25141 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
    25201 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
    25261 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
    25321 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
    25381 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
    25441 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
    25501 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
    25561 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
    25621 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
    25681 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
    25741 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
    25801 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
    25861 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
    25921 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
    25981 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
    26041 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
    26101 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
    26161 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
    26221 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
    26281 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
    26341 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
    26401 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
    26461 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
    26521 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
    26581 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
    26641 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
    26701 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
    26761 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
    26821 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
    26881 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
    26941 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
    27001 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
    27061 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
    27121 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
    27181 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
    27241 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
    27301 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
    27361 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
    27421 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
    27481 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
    27541 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
    27601 caaagcaggt tgttccatta aataaagaat agg
//
LOCUS       DACCCM010000032        27311 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCCM010000032 DACCCM010000000
VERSION     DACCCM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.033"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            647..964
                     /locus_tag="IVK78_001383"
     CDS             647..964
                     /locus_tag="IVK78_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6374863.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IVK78_001384"
     CDS             complement(1055..1420)
                     /locus_tag="IVK78_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374864.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IVK78_001385"
     CDS             1628..2110
                     /locus_tag="IVK78_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374865.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IVK78_001386"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IVK78_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP6374866.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="IVK78_001387"
     CDS             complement(3484..4758)
                     /locus_tag="IVK78_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6374867.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="IVK78_001388"
     CDS             5031..7502
                     /locus_tag="IVK78_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6374868.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="IVK78_001389"
     CDS             complement(7605..9341)
                     /locus_tag="IVK78_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP6374869.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="IVK78_001390"
     CDS             complement(9338..10042)
                     /locus_tag="IVK78_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6374870.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="IVK78_001391"
     CDS             10173..10676
                     /locus_tag="IVK78_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP6374871.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="IVK78_001392"
     CDS             10636..10974
                     /locus_tag="IVK78_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP6374872.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="IVK78_001393"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="IVK78_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP6374873.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="IVK78_001394"
     CDS             12525..13103
                     /locus_tag="IVK78_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP6374874.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="IVK78_001395"
     CDS             13107..13628
                     /locus_tag="IVK78_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6374875.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="IVK78_001396"
     CDS             complement(13707..14261)
                     /locus_tag="IVK78_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6374876.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="IVK78_001397"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="IVK78_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP6374877.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="IVK78_001398"
     CDS             14801..15052
                     /locus_tag="IVK78_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6374878.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="IVK78_001399"
     CDS             15177..15968
                     /locus_tag="IVK78_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374879.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="IVK78_001400"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="IVK78_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP6374880.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="IVK78_001401"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="IVK78_001401"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP6374881.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="IVK78_001402"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="IVK78_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP6374882.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18266..18928
                     /locus_tag="IVK78_001403"
     CDS             18266..18928
                     /locus_tag="IVK78_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374883.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            19007..22234
                     /gene="fss3"
                     /locus_tag="IVK78_001404"
     CDS             19007..22234
                     /gene="fss3"
                     /locus_tag="IVK78_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6374884.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            22348..22662
                     /locus_tag="IVK78_001405"
     CDS             22348..22662
                     /locus_tag="IVK78_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6374885.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            22675..23049
                     /locus_tag="IVK78_001406"
     CDS             22675..23049
                     /locus_tag="IVK78_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6374886.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            23059..23403
                     /locus_tag="IVK78_001407"
     CDS             23059..23403
                     /locus_tag="IVK78_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6374887.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            23474..24619
                     /locus_tag="IVK78_001408"
     CDS             23474..24619
                     /locus_tag="IVK78_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6374888.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            25334..27193
                     /gene="ltrA"
                     /locus_tag="IVK78_001409"
     CDS             25334..27193
                     /gene="ltrA"
                     /locus_tag="IVK78_001409"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6374889.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         9289 a         4756 c         5580 g         7686 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatcagaaga taaacacgga aaacttcctt ggaaatctga aatttgtgaa aagtccttgt
    17941 atatcaaggg ctttttcttt tttctctatc acagttttaa gccatttttg gctgttttgg
    18001 accatttttc aatcatattc ccttaaaaat tgtcctagat tgtccgacat tgactatcag
    18061 tgtcgtcaaa atagggcaaa atttagtagt cagcttgacg acaaatataa tacgaaaaat
    18121 accaaaacat ttaccgaatt tagcaacttt tgaacccaag tttctcaaag ggtcagctat
    18181 tagcaaaaaa gacaaaacat gtacttttct actaaaaagt tgatgtgata ggcttctatc
    18241 taaataacta ttaggagttt ttatcgtgaa gcaaaatgat ggacgtatca tttggaaaaa
    18301 tcaaagtgag ttaaaactga ttttaacaat taatgaattc atcgaaaaac acggaattac
    18361 atctagccgt caataccaga aaaagctatc agaaaatcca aattctgcac caagtatgtg
    18421 gttcattaac aaaaaatatg gatcatggga gaatttattg attagtatag gacgagaaaa
    18481 tactgggtat ggaaaatggg ctagaatgtc agaacaagaa ttattggaaa tagtagaagc
    18541 ctttattaag tgcgagaaga taacctctca acgaatgtac gaacaaaaat cagtagggaa
    18601 gaatattcct tcacttagta ccattaaaaa gatgttagga gatatacgtc cactttttaa
    18661 agaaaaaaat gatggatctc gatttactga tttcgaactg ctgttagaac taaaaaatga
    18721 aataatccga ttaaaactac aggatgattt atcaatgaca aagtttcgaa aactagttca
    18781 atccccaaaa ctaccatcag ttgatacaat tatgaaaaga acgaacaaaa attgggagga
    18841 attgatggca gagattggat ttgattatcg aagaatcaaa atttataagc agagaaacaa
    18901 cctatccaag acaaagaaaa ctaaatagta tcactctaaa tcagacgttc aaaattaaaa
    18961 tttggacgtc ttttttatta cccaaaaacg aaaggagatt tattttatga aaaagccaag
    19021 atttaagaat tggcgtttgc tcgcaacact gagtctatta tgccaaacaa ttggtggatc
    19081 gcttggccca acgattgcct ttgctgatga aattactcat ccacaaacgg taactgtgga
    19141 attggatttg gctcaccagt atgctgtcga aggtacattt agcgatggtc gtcccatgtc
    19201 agaagttacc gttccgcact atgcagttta caatggtgtg aagcaagata ttttctgtat
    19261 tgagccaggg gtgccgattt acaatgagtt cactcctggt tatgagaaaa atccattgcc
    19321 tgatatgtcg gagaaagcga aattagtttc cgttttatgg aaggaagctg gtacggatgt
    19381 ggatacacat attgtggccc agaagatgat ttggcaagaa gttaatggct acacgcttca
    19441 ctcaattaaa cgttctgatg gtagtgcagt aaatatcgca gccattgagt caaaaatcaa
    19501 tcaagccatt gcggattatc aaaagaaacc aagcttccat aactccacag tgaaaaccgt
    19561 gttaggtcaa tcgactactg tgacagatac gaacggcttg aatttatcgg agtttgatga
    19621 agttgtcgag aatatcgcaa atattgatta tcgtgtgaac ggcaatcaac tagtgattac
    19681 tccaaatgcc aattcaaaag agagtggtga attaacccta aagaagtcag ctaatacagg
    19741 aacaccggta gcttataaaa tggctggtca acaaactttg atggcggggg caattgataa
    19801 gcccaatacc tacacaatca aaattgatgt ggaaactgag ggttctttga agattaaaaa
    19861 agtcgataaa gaaacaggaa atgttgtacc gggaaccgtt ttccatttag actttggaaa
    19921 aacattacct gcaaaagacg tgaccaccga taaagaaggc attgcgacat tggatgagat
    19981 tcctcatggc gcgaaagtaa ccattactga aaaatcagtg ccagctcctt atacgattga
    20041 cactacacca atcaccgcaa ccattaaatc tggtgagact atttctgtga cttcaaaaaa
    20101 tactcgcgaa aaaggtcaaa tcattttgaa taaaatgggg gtggaaacag gaactgatct
    20161 ttggaacgac aaatactcat tagccggaaa tacgtttgcc attcgcaaag acagtccaac
    20221 cggtgaaatt gtccaagaaa tgaccacgga tgaaaaaggt catgcggaaa caccaaaaga
    20281 gattgccaat gcgttggaat tgggaaccta ttatgtgacc gaaaccaaag ctagtaatgg
    20341 ctttgtgaat acctttaaac cagtaaaagt cgaattgaac tatgtcaatc aaaccgtggc
    20401 tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatc actggtgaaa ccactttgac
    20461 aaaagaagac aaagataccg gtaatgaaac tcaaggtaaa gctgagttta aaggagctga
    20521 atatactctc tttactgcaa aagatggtca agcggtcaaa tggagtgagg cttttaaacc
    20581 agagttagtg aagggaacga aagcttctga tgaaacggtg actttgactt tagatgaaaa
    20641 gaatcaagtt gctgttaaac acttagcgat taatgagtat tactggcaag aaaccaaagc
    20701 acctgaagga tataccttgg atgaaacaaa gtaccctgtt tccatcaaaa aggtggatga
    20761 taacgaaaaa aatgccgtga ttacccgaga tgttacggca aaagaacaag tcattcgctt
    20821 tggctttgat ttctttaaat ttgctggatc agctgctggg actgccgaaa ctggcttcaa
    20881 tgatctgacc tttaaagtat cgccattgga aggaaccagt gagattacag gtgccgaaga
    20941 tgaagcaatt acagcttata acgagcaact aggttttgat ggctatggca agtttgaaaa
    21001 tcttccttat ggggattatt tacttgaaga agtagaggct ccagaaggat ttcaaaaaat
    21061 tacgccatta gaaatccgtt ctacttttaa agaaaataaa gcggattaca ctaagagtga
    21121 atacattttt acgattaccg aaaaaggaca aaaacaacca attaaaacgg tgaccgttcc
    21181 ttacgagaaa ctgacgaata aagcattttc tgttagcttg aatcgtttga tgctctatga
    21241 tttacctgaa gaagaagata gcttaacttc tcttgggact tggaaagacg gaggcaaaga
    21301 attgacagac cttgattcta ccgagctagt tgataaattg agctataact tgcatgaaat
    21361 caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gaagcgacaa aagctgccca
    21421 agaaaaagat gaaaaagcca aaccagtggt aattgccgaa acaaccgcaa cgttggcgaa
    21481 taaagagaaa acaggaactt ggaaaatttt gcataaatta accgcagaac aagttttgga
    21541 taaaaccatc gtcttattca actatgtgta tgaaaacaag aaaccctatg aagcaggtga
    21601 tgatccagta gcgaaagatg ctagcttgaa caatcaagcc caaactgtca attgtacggt
    21661 ggaacgccat gtttccatcc aaacaaaagc ccacctagaa gatggttcgc aaacctttac
    21721 tcatggggat gtggtggata tgttcgatga tgtgtcgatt acccatgatg tactggatgg
    21781 ctcaaaagaa gcttttgaaa caattctgta tgcattactt ccggataata cgaccaaaga
    21841 aatttggaaa tctggcaaaa ttgattatga agtgaatgac aaagaattca ctaaaaccgt
    21901 acttgcggaa aaagtggaca caagtaaata tccagaaggt acaagtttta ccttcaaaga
    21961 aattaactat ggcaaagacg gaaacatcaa tgggaaacac aatgaggatc taaaagaaaa
    22021 aactcaaact ttaacaccta aaaaagttcc gacaacacca agtgcaccag aacaaccgga
    22081 aataccaact gttccaagtg actctcaaga atctagtcct acagtaaaga cattcccaca
    22141 aacaggggag aaaaattcta atgttctgct gtttattggg tttaccttga tctttgcgac
    22201 tgctgggtat tatttctgga atcgccgaaa ctaaggtgat gtgatgaaac aactaaatag
    22261 gaaaaaccaa cgtgcgcgac cgcctcctaa aaaaagtggt cgcgctattt ttgtatccaa
    22321 aactaaaaat gaaaagagga aaagaagatg gaattgaaat ttgttgtgcc agatatggcc
    22381 gaaacctttg gaaagataag gtatgccggc gaaggcgaag ttttaactga aggatatggg
    22441 cggaatacta cagtaattgg tcgtagttat catttgtatt ccagtaagca acgagccgat
    22501 gatattgaag tggtggtagc tgcggaagcc ggcgaaaagg attttgatca agatcaacca
    22561 ctaaaagccg tgaatcccca tttggttgcc aaaggctatg agattgagaa tcgtgggttt
    22621 accgactacg tgttgtatgt cgatgattta gtgaaagcat aggaggaaaa gaagatgaga
    22681 ttaacagaag gtattgttgt agattcagga ctaacgtttg ggaaattacg attttccgca
    22741 ttacgccgag aagtacggaa gcaaaatgag gatggaacgg ttagtaacga ggtaaaagaa
    22801 cggacttata atttgaaatc ctctgcgcaa ggtcgaatga tccaagttag tatcccagca
    22861 aatgtaccct tacgcgagtt tgcctatgac gcagaggtag agttggtcaa tccaatcatg
    22921 gatacggtcg ccaattatgt tttccgagaa ggaactactg tcaattggtt cattaaggcc
    22981 gatgatttag tcttgaaacg acaaccagat cagggaaatt ctacaaacca aaatgaagga
    23041 aagaaatagg tgaagcgtat ggaaattaat gttgaacata ttttagattg tctcgatcaa
    23101 tatggaaaag gtgagctaac ggaggaacaa ctcactaaag cattaaccta tgatgaaaaa
    23161 atgtttctga ttatgcatca agggttactg gaagtaggta acgatgccga agaagatttt
    23221 gatgtactga attggttagg ggaagaagag tccttcttta tggttatcga agtaaatgaa
    23281 agtctatgtc gagaagccga aaccgtattg gaagaaattg gtgtggaaat gccggatgcc
    23341 attgaagttt ttttaaagca actagtggaa acgaaacaac tccctatagc agtaaatgac
    23401 tagcataact gcttgaaatt ggatcaatga aatcctatga agcttttttc gattggaggt
    23461 ggaaaccata aaaatgtaca aagggcatcg tatacgggca ggagatcagc acttggttta
    23521 tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt
    23581 tcaagagctt agacaatttg atatttctaa gctatcactt tctactatag aaatcgttcg
    23641 ttctataaaa gatttaatct atttgctagg aagtctcgtt ctttcaggaa gcacgatgtt
    23701 gctttatata cacttttttc aggatcattg gcgcagtttg tggcatcgac aaaagctagc
    23761 ccggatgatt ctagaaaatc attggtacga agtaaaacaa actcagagtg aaggattttt
    23821 caaagacctc aatagtagtc gaaccaaaga gacaatcagt tacttcccca aaatctatta
    23881 ccgtatgaag gatggtttac tttctattcg cgttcaaatt tcactgggaa aatatcaaga
    23941 tcagctttta aagctggaaa agaagttaga aagtggtcta tattgtgaac tagtagaaaa
    24001 ggaactcaaa gactcctatg tggagtacac tttactgtat gatataattg ccaatcgaat
    24061 tgggatagac gaagtagtgg cagaaagtgg ggctttgcga ttgatgaaaa atcaagtatg
    24121 ggcttatgat tctttacctc atatgttaat cgctggcggc acaggtggtg ggaagactta
    24181 ctttttactc actatcatcg aggctttatt aaaatctgat gccgagcttt ttgttcttga
    24241 tcccaaaaat gcggacttag ccgatttagg tacggtaatg cctcatgtct attcccaaaa
    24301 ggaggaaatt tctgcttgtg tggaagattt ctatgagcgc atgatgactc gtagcaaggc
    24361 aatgaaagaa atgcccaact acaaaacagg agagaattat gcgtttcttg gacttccacc
    24421 aaacttttta atctttgatg aatacgtggt ttatatggaa atgttaacga cgaaggaaag
    24481 tgcggtgatt ttgaataagc tcaaacaaat cgtgatgtta ggtcgtcaat ctggtttctt
    24541 tctgattcta gcttgtcaaa gaccggatgc gaaatattta ggagacggga ttcgcgatca
    24601 atgtgcgaca tgtttctaac tgaaaaggtt aggcacttaa aagaagaaaa cataagtttc
    24661 cttttaagga gaactgatat tttgagcagt ggaatgatgg ggtaacgccc tgaaacgctc
    24721 ccttaatact ccgactggct gtttaacata tagctgttag atggtcagaa gctcggtgaa
    24781 gtcggctgaa agttaccgta aatatgataa attcatatac gaaagttgtt cagaggtgaa
    24841 tgatgtaacc tataggtcgg aggtctataa aatactcacg gtgagaatgt aagtaatact
    24901 actgacgaac ttgcgaatga aagaatctat acacggactg tatagaaatg tacaggattc
    24961 taccgtatca acggaacaga atgtatgttg tggtttagta agattattgg ttatgaacaa
    25021 ccatgcaatg ttacaggcat tggaaatata caggttcaaa gcaagcacct aaaagtatat
    25081 ataaagataa aaatatcgaa acatgggaag ctggcaatgt ggaggaaact atattccatt
    25141 gaaacatttg tgacgaagaa taataagcac atttatgcca gtgaaagtgg tggcacagta
    25201 ccaatgagga tatagaaata tatcggagga atagccacta gtcaaaagaa atcgttaatt
    25261 aaatgatgta tttcatagaa tcgagtaaga caaaataaac actacaacct aagtaaacaa
    25321 aggtagtggt gctatgaata aagtaaaaac aaataaaaca cttcgtcata gtgaatacta
    25381 tggcatgaca caaatatttg atgaactgta tgtaaaaagt gataaaaaag aagtttttac
    25441 agagctcatg acaattatta tgagtgatga aaatattcga ctagcataca gaaatatcaa
    25501 gaataacaag ggcagttata cagcgagtac cgataagaaa agtattcgag acattaaaac
    25561 actgtcagaa gatacctttc taactgaggt taaagttctt tttcgctgtt acaaaccacg
    25621 agcagttaga cgtgtagaaa ttcccaaacc aaatggaaaa acacgccctc tgggcattcc
    25681 atcaatctgg gataggttaa tacaacagtg tattttacaa gtcttagaac cgatatgcga
    25741 agctaaattt cacaattata gttatggatt tcgaccaaac cgttccacac atcatgcgat
    25801 tgctacctgc aatcgactgg caaatagggg gaagctacac tttgtagtag atgttgatat
    25861 taaaggattc tttgataatg tcaatcactc taaacttttg cgtcaaatgt ggacgattgg
    25921 aatccgtgat aaatggctat taggtattat tcgatccatg ctaaatgctc ctatagtcct
    25981 accaaccggt aaagtcgaac accctaaaaa aggtgtacct caaggtggtg tcctatcccc
    26041 tttactatct aatatcatat tgaatgaatt agattggtgg gttgcttcac aatggcaaaa
    26101 taccacgatt gagacagaaa agattatgga taggcgagat aaaggcaaag gcattgataa
    26161 gactaatcag tatcgggcat taagacgaac aaacttaaaa gaaatataca ttgttcggta
    26221 tgctgacgac ttcaaaatct tttgtagaaa acgaagtcat gcagataaaa tatttcacgc
    26281 aacgaaaatg tggcttaaag aacggttatc acttgaaatt agcgaagaaa aatccaaaat
    26341 tgtcaatctc aaaaaaggta aatcagaata tctaggattt gaattaaagc taatcaagaa
    26401 aggtaaaaaa tacgtagtcg aaagtcatat gagcaaaaaa tcaatgacaa gagttaaaat
    26461 ccaattaaag aaacaattac gtaaagttgc cagaccgaag aatcattgcg aacaagcaaa
    26521 agaaattgga ttatataatt ctatggtgat tggaatccat caatattatg gaattgcaac
    26581 ctgtgtaaat ctcgattgca gtaaaatagc ctatacacta aaaccgttca tcaatcacaa
    26641 actgccgaca aagaaacatg gaaagatatt aaatacattt atcaaatcga agtatggaaa
    26701 aagtaaagaa atgcgatggt tgaataatgt gccaattgta ccgctcaact atgttcagtt
    26761 tagacttgct gtccccctat cagagggaac ttgcaagtac actgaaagtg gacgaagcaa
    26821 gatacacagt aaattaaaat tagacctagc ccttttactt cacatgatga gaaatagtaa
    26881 ttatgaaaga agtattgaat ttgtggataa cagaatatct aaatattctg ctcaaaaggg
    26941 aaaatgtgct atcacgggta aatttctcga atacgaagaa atccattgcc accatatcgt
    27001 accagtaaaa caacagggta cagacaaata cagcaacctc attattattc acaaaaatgt
    27061 tcacgcatta gtccatgcta tcgaagaaaa aatcatacac aaatacctga atgtattaaa
    27121 tctaaccaat gaacaaatag aaaaactaaa tgaattacgt gtcaaagctg gtaatagtgt
    27181 tttaacagtt taacttgaaa aatatatcat acttaacatt cttttgatgg aacgccgtat
    27241 gaagtgaaag tttcatgtac ggtgtgaagc gggggaaaat ctggagatga catcaaagga
    27301 ttacctatcg c
//
LOCUS       DACCCM010000033        26902 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCCM010000033 DACCCM010000000
VERSION     DACCCM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.034"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(40..1146)
                     /locus_tag="IVK78_001410"
     CDS             complement(40..1146)
                     /locus_tag="IVK78_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6374890.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1356..2357
                     /gene="ccpA"
                     /locus_tag="IVK78_001411"
     CDS             1356..2357
                     /gene="ccpA"
                     /locus_tag="IVK78_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP6374891.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2694..5039)
                     /locus_tag="IVK78_001412"
     CDS             complement(2694..5039)
                     /locus_tag="IVK78_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6374892.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5318..6220
                     /locus_tag="IVK78_001413"
     CDS             5318..6220
                     /locus_tag="IVK78_001413"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP6374893.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            6433..8208
                     /locus_tag="IVK78_001414"
     CDS             6433..8208
                     /locus_tag="IVK78_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP6374894.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8274..9860)
                     /locus_tag="IVK78_001415"
     CDS             complement(8274..9860)
                     /locus_tag="IVK78_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP6374895.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            10111..10335
                     /locus_tag="IVK78_001416"
     CDS             10111..10335
                     /locus_tag="IVK78_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP6374896.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10476..12227
                     /locus_tag="IVK78_001417"
     CDS             10476..12227
                     /locus_tag="IVK78_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374897.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12227..14011
                     /locus_tag="IVK78_001418"
     CDS             12227..14011
                     /locus_tag="IVK78_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374898.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14337..15188
                     /locus_tag="IVK78_001419"
     CDS             14337..15188
                     /locus_tag="IVK78_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6374899.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15285..17165
                     /locus_tag="IVK78_001420"
     CDS             15285..17165
                     /locus_tag="IVK78_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6374900.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(17205..18107)
                     /locus_tag="IVK78_001421"
     CDS             complement(17205..18107)
                     /locus_tag="IVK78_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6374901.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18338..19741
                     /locus_tag="IVK78_001422"
     CDS             18338..19741
                     /locus_tag="IVK78_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6374902.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19741..21174
                     /gene="ascB"
                     /locus_tag="IVK78_001423"
     CDS             19741..21174
                     /gene="ascB"
                     /locus_tag="IVK78_001423"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6374903.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21319..22245
                     /gene="trxB"
                     /locus_tag="IVK78_001424"
     CDS             21319..22245
                     /gene="trxB"
                     /locus_tag="IVK78_001424"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP6374904.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22459..24186
                     /locus_tag="IVK78_001425"
     CDS             22459..24186
                     /locus_tag="IVK78_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6374905.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24258..24551
                     /locus_tag="IVK78_001426"
     CDS             24258..24551
                     /locus_tag="IVK78_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6374906.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24643..25503)
                     /locus_tag="IVK78_001427"
     CDS             complement(24643..25503)
                     /locus_tag="IVK78_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP6374907.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25595..26023
                     /locus_tag="IVK78_001428"
     CDS             25595..26023
                     /locus_tag="IVK78_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6374908.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26185..26361
                     /locus_tag="IVK78_001429"
     CDS             26185..26361
                     /locus_tag="IVK78_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP6374909.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26388..26834
                     /locus_tag="IVK78_001430"
     CDS             26388..26834
                     /locus_tag="IVK78_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP6374910.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8514 a         4797 c         5747 g         7844 t
ORIGIN      
        1 aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa
       61 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
      121 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      181 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      241 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      301 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      361 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      421 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      481 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      541 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      601 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      661 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      721 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      781 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      841 tacattgcca ggctgaccat agcgagtacg aagttctttt ccgatgatct cccaagggtt
      901 ttcgctgtct aaatagccgc ggacatcata cgcccaacta ctgttttgag catcttcaac
      961 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
     1021 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1081 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1141 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1201 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1261 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
     1321 cagaaaacat ataaaatatg aatgggagaa cacaaatgga aaagcaaaca atcactatct
     1381 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1441 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agtcatcgat cgcttggatt
     1501 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggggtaa
     1561 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1621 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
     1681 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
     1741 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1801 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1861 aagatgcagt aaataaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1921 cgttgatcga tcctatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1981 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     2041 gtctatctct agccgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2101 atgaattagc catcggtatt ttggatggtt tattagatcg tggcgtgaaa gtaccggaag
     2161 gattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2221 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     2281 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2341 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgagcctg ccgtttaact
     2401 ctgcgttaca aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat attgtgactc
     2461 gtgattcgga gttggcagga gaacaccgtt tattcgggaa aaggggttat gacaaaattt
     2521 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2581 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     2641 ggactgtgac aatactattg tcacaatctc ttattactgt gtattctatt aaatcaatca
     2701 tctttctcat ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc
     2761 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     2821 ggtccatttt tcgcccatag cgattcaact gtttttccag gtgtttggat ggatttttta
     2881 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     2941 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     3001 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     3061 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     3121 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     3181 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     3241 ctgcgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     3301 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     3361 tcttcataga cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg
     3421 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     3481 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     3541 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     3601 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     3661 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     3721 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     3781 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     3841 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     3901 atgtatccat aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc
     3961 gaatggattg tatatccttt attttggatt gtttgctgtg ccttttgata ataagcgtca
     4021 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     4081 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     4141 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     4201 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     4261 gaaagatctt cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg
     4321 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     4381 attcctcttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     4441 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     4501 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     4561 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     4621 gatactaacg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     4681 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc
     4741 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     4801 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     4861 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     4921 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     4981 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     5041 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     5101 cagacaaggc tgtttttggc aggaatttct tttttaaagg agttaaaacg tgagaaaatg
     5161 atctttttta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     5221 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     5281 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     5341 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     5401 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     5461 gaatattgat gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca
     5521 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     5581 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     5641 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     5701 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     5761 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     5821 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     5881 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     5941 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccgaca
     6001 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     6061 aatgtttgac aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa
     6121 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga
     6181 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     6241 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt
     6301 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt
     6361 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     6421 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     6481 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     6541 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     6601 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     6661 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     6721 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc
     6781 gtgaagaaca agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt
     6841 tttacacatg aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag
     6901 atcattatcg cttgtttact agctatttat taccgcaaat acggttctag cagaaaattg
     6961 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     7021 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     7081 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     7141 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     7201 gtctatcagt ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     7261 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     7321 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     7381 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     7441 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     7501 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     7561 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaaccat caaaaagaaa
     7621 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct accaatcgta
     7681 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     7741 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     7801 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     7861 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     7921 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     7981 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     8041 gtttttcttt tcggtgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt
     8101 ttaagtgtct acactggcgc aggcgtgccg ttaacgcaat tcatcgtagt aacaagcatg
     8161 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     8221 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     8281 atttcttcta ccatttcctg caaacgctgt tcatattttt tgtttccttt atataaatca
     8341 ccaatcgtcc atgccaacag gaggaggaca agaacaatga ttacataagc gatgtgatca
     8401 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     8461 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     8521 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     8581 gggatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     8641 ttcaaagcgg tatgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     8701 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     8761 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     8821 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     8881 atcaagatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     8941 gaaggatctt gaacagaacc ggttttgaag acagcgacac ccataatcaa tagcagcgca
     9001 ttgactagaa aagccatcga caattggata ttagagtccc aagtagaaaa acgtacagct
     9061 tgtgctacat cttcttcgtc ttggtgatcg atcttccgag tttgtgaaac tgctgaatgt
     9121 agatataagt tatgtggcat gactgttgcg ccaataatcc ctaatgctcc agttagcgga
     9181 gtttcgccac caatgacagg tgagtcggag aatgtatggc tgttagggat aaagccagca
     9241 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     9301 acgaaaatca agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     9361 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     9421 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     9481 aattcagtca agatccataa aatgacaccc aatgtcttac ttgtgcgtgc tcggattgct
     9541 tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatactg taaaagcata
     9601 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     9661 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     9721 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc cactgttcca
     9781 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     9841 ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     9901 cacctcaaaa agaaaaaatt aaagaaaaag attcggtatg cctaattttt tttcgtggcg
     9961 tgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
    10021 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
    10081 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
    10141 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
    10201 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
    10261 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
    10321 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
    10381 ttttatttcg taatttgtta gactattcag attattcagc aaaaagacaa gcacagacaa
    10441 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aagttaggct
    10501 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
    10561 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag attgccgata
    10621 aaaatttatc agtaaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
    10681 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcggttgg
    10741 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
    10801 aaaaatatcg gactggggat ttgatggctc atgcaacgaa tgatttgaat gcgatccaga
    10861 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
    10921 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
    10981 ctttgctggc cgtcacttca cgagtgctgg ggtcgaaact ccatgatgct tttcgtgatt
    11041 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
    11101 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
    11161 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
    11221 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
    11281 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
    11341 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
    11401 atcgcgtcaa tgaacttttg catgaaaaaa cgcatatcgt ggaacaactg ggtgcagtgc
    11461 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
    11521 aatcaccagc attagaacaa atcaagtttg caatccaaga aggagaaaca ttaggtattg
    11581 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
    11641 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
    11701 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
    11761 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
    11821 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
    11881 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
    11941 tcaaaaatcc tgaattatta atattagatg atgcactttc tgcagtagat gccaaaacag
    12001 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcatttcag
    12061 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
    12121 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
    12181 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
    12241 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
    12301 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
    12361 tctgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
    12421 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
    12481 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcttcatt
    12541 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
    12601 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
    12661 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
    12721 ttcattcatt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttctt
    12781 gccaatttta ggaatagtca tctggtatta ccagaaattt agttctcgta tttaccggaa
    12841 tatgcgggaa aaattaagtc agctgaatac gaaattgaat gaatcgattt ccggcatgcg
    12901 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
    12961 tgagtattta gacacgcgtt atgcgatgat caaaacaaat tcgattctgt taagtccgat
    13021 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
    13081 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
    13141 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
    13201 tggtagtcgt atcttgaaga ttatggatac agaagaatat gcaccacaac aaaaccctgg
    13261 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
    13321 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
    13381 attagtggga cacaccggaa gtgggaaaag ctcgatcatc aatgtgttga tgcggtttta
    13441 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
    13501 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
    13561 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
    13621 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
    13681 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
    13741 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
    13801 aacagaagga ttgatccaag aaggattggc gaaaatgcga caaggacgga caacgattgc
    13861 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
    13921 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
    13981 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtagc attttgacct
    14041 aagcactaga gtgaaaataa aacatagctt cccatatttt tcatctgtga gagttagtgt
    14101 cgtagttacc agatttaaaa aagacgacgt cagctagtca gaagtgccta atagccccta
    14161 agaaagcgtt gacaaaatcg tttgctttcg ataatataaa gaaaacgata caacttggat
    14221 agtgattgcc tgatgcaagc taaacctaat gagtctccca tggtttcatg gacggctttt
    14281 tgggtttttc tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc
    14341 tgattgagaa agtactgaat aacaacgtcg taataacaag ggatgaaaga gatcgggaaa
    14401 tgattgtgat gggaaaagga ttggcattca aaaagaaggt gggtgacccg attcttgatt
    14461 catgcatcga caaaaaattt cagttgacaa atgacggact ttcacaacag ttccaagaat
    14521 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
    14581 aagaggctct gcaagtccat ttaaatgatt ctgtgtatat ttcattgacg gatcacttgt
    14641 atacagctat cgaacgtcaa aaaatgggga tctttgtgcc aaatgtattg ctgtatgata
    14701 tcaaacaact gtttcctaaa gagtatgcca ttgggaaaaa aacagtgaag aaaatctcgg
    14761 ataaatacca catcaagtta tcagaagacg aagccggatt tatcgcattg catttagtca
    14821 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttaccaca acaatacagg
    14881 atattttaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccact
    14941 tttttcgatt cgtcacacac ctgcgttttt ttctgacacg tgttctagat ggagcgaaat
    15001 cagaagatgg agaagtttct gaggaattgt tccaagttgt tcaaaaaaaa tataaggata
    15061 cgtttggttg cgttgagaaa atagcagact ttttaaccgg ggctttcgac taccagatgt
    15121 ccacggatga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    15181 gtaaataaat aaaaaaggag gacaaattaa caagaacgta tgaaaataag ggtagtgaca
    15241 ttcagttggc taaaccttcc actaattaat tggagggaat agaaatggga aaataccagg
    15301 aattagcaga aaacatcgta aagaatatcg gaggaaaaga aaatatccgt agcttgacac
    15361 actgtgtgac gcgacttcgt ttcaaactaa aggaggaaaa taaagcaaag gatgatgttt
    15421 tgaaaaagat ggaagggata gtcaccatca tgaaaagcgg cgggcaatat caagttgtaa
    15481 tcgggaatca tgtgccagat gtctatgcag aagtactgac agtagccggg atttccgatg
    15541 aagtagaaga agaggtttct tcaagtagta atctgttcaa tcgattaatc gacatattaa
    15601 gtggctgttt ccagcctttt ttaggtgctt tagctgcagc gggaatggtc aaaggattaa
    15661 atgctttgtt cttgtttctc aaattttata ctgaaggatc gggtacgtac atcatgctga
    15721 atgggatagg ggatgcaatt ttttatttca tgccagtgat cttagggtac acagcagcga
    15781 aaaaatttca tctgaatccg atggtggggc tcgtgattgg tgcagcactt tgttatccga
    15841 ctgtacaagg agcagcacta caaagtggtt tccctgatgg cagtcctgcg ccttatagct
    15901 tgctgggtct tccggcttat gaaacatttt tgggtatccc atgggtgggc agcagttata
    15961 ccagtagcgt cgtacctgtt atcttcatta tcgcatttgc cggacaagtc caaaaaattg
    16021 ccaagaaatg gattcctgca gtcgtccaga cctttttggt tcctttttct gtgttattaa
    16081 tcgcacttcc tgctggcttt ttgattatcg gtccgattgt cagtatactg acagatctat
    16141 taagcacggg attccaagca ttgatggact tttctccagc tgtgtatggt gcagttctag
    16201 ggtttctttg gcaagtactg gtgattttcg gacttcattg gagtattatc cctcttgcga
    16261 ttatgcagct agcgcaaaat gggtggagtc agatgttagt aggcgtttat ggcgcgagtt
    16321 ttgcacagac agcagtggta ttggcgatgt atttcaagtt caaggatcaa aaaatgaagg
    16381 cgctttgcat gccagctgtg atttcaggaa tcttcggcgt aacagaaccg gctatttatg
    16441 gaatcacctt accgaagaag aagccattta tttactcgat gattggtggt gccatttcag
    16501 gaatctattt gatggcatct ggaacaatca gctacacaat gggcggatta gggatttttg
    16561 gggtggtaaa ctacatcacg gaaacagacg ccagtggtat gtttcaagcg tttatcggca
    16621 ttgctattgc ttcggtaatc gggttttcgc tgactttttt cttttggaag gatgaagcag
    16681 gtgaaacaga tgctatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag
    16741 gacaaatcgc tccactaaat gcagcgaagg atgcagcttt tgcacaagga acactaggtc
    16801 gaggaatctt gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca
    16861 tgaccctgtt cccgacgaaa catgcaattg gaatggtttc tgaaacaggg ctggaattgc
    16921 tgattcatgt cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc
    16981 agcaaggaga aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg
    17041 aaaaggaagg atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg
    17101 atattttgga aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga
    17161 cctgaacggt taggtgaaga atggaatagg cttaactatt ctttttattc cacttcttta
    17221 ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt
    17281 tgaccgacag aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta
    17341 tataacacgt ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa
    17401 tacagtcgct cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa
    17461 tcaccgatac tggtcaaagc taaaattgga acatgatttt gtttcaatat ttctgcggta
    17521 cgaagaatca ctttattttc acccgtataa gaaatcagga tcccgcacat atcgctcgtg
    17581 caattgtacg cttcatagaa tgattcacca aaagtggtga caattgtgac atccattttg
    17641 atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaag
    17701 atcacgattt tttttgcctg gagcagtaaa tacttggctt tttgcagttg gtcatgatga
    17761 atgagagaaa gtgtatcttg aatcgtcatc tgttctaaag aagccatttt atttgcgatc
    17821 gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct
    17881 tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc
    17941 attttttttg ctacacggat gagtgtagac gggtggacat agcatcgctc agcgatctct
    18001 tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt
    18061 tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta gttaccttct
    18121 tcttattttt ttacgtaaat tataactttt ttgaaaatgt tttgtaaacg gatttatact
    18181 atataactta aaatagactt tttttgacaa gatatttaca aaaatccact agaaaacagt
    18241 aacttttgta aatgttactg gaaagaaacg gatttctatt gtttgttttg tttacaaaaa
    18301 agataatgaa agtgtaaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc
    18361 tacgattggt ggaggcagca gttacacccc tgagttgatg gaaggattta tcaaaagata
    18421 tgaggaatta ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct
    18481 tgagatcgtt agtcagatgt ctcagcgtat gtgggatgct tctccgtatg aagtcaaaat
    18541 ctatgcaact ctggatcggg aagaagcatt gaatgatgcg gattttgtta cgactcagtt
    18601 tcgtgtcggt caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat
    18661 gattgggcaa gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt
    18721 ggtcttggca atcgttgaag acatgaagcg tttatgtcca aatgcttggc tgatcaattt
    18781 cgcgaaccca agtgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt
    18841 gattggatta tgcaatgtgc cagtaatggc gatgatgaca gaaccagcaa tgcttggcaa
    18901 gaaaaaagaa gagctgatct acaagtttgc cggtctaaat catttccatt ggcacaaagt
    18961 agcagataat aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga
    19021 caatggattg ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat
    19081 gaatatgatc ccttgtggct accaccgcta ttactaccgt gaagaagaaa tgctggaaca
    19141 tgctttagaa gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc
    19201 agagctattt gaattataca aagatccaga attagaccat aaacctgaac aattgcaaaa
    19261 acgaggtggc gcttattatt cagacgctgc ctgtgaaacg attgcttcaa tctatggaaa
    19321 taaagaaaca cagatcgtgg tatctacaaa gaatgatgga gctgttcctg acctgccggc
    19381 agattgtgta gtagaagtca ctgcctatgt tggcgcacaa ggagcgagaa atgtagcgtt
    19441 tggttctctt cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct
    19501 gacgatcgaa gcagctgtga ctggtgatta tgggacagct ttacaggcgt ttacgatcaa
    19561 tccgcttata cgatcaggaa agacagcaaa acggatcatg gatgagttgt ttattgcaca
    19621 ccagcagtat ttaccgcaat tcaaggaaac aatcagccga ttaaaagaag aaggaatcga
    19681 agttcaagat gaactggcaa gagaattatc aggtaataca catataatgg aggaagtata
    19741 atgggattca gaaaagattt tttatggggc ggtgctacag cagccaatca atgtgagggc
    19801 ggttatgatg aaggtggacg cggactagca aacgtagatg ttgtgccaat cggaaaagac
    19861 cgttttcctg ttatcaccgg aaagaaaaaa atgtttgaat ttgatgagga tcatttttat
    19921 ccagcaaaaa ttgcaatcga tatgtatcat cgttataaag aagatattgc tttgtttgca
    19981 gaaatggggt tcaagactta tcgaatgtcc atcgcctgga gtcgtatttt tccaatgggg
    20041 gatgaaaaag agccgaatga agctggcttg aaattttatg aggatgtctt taaagaatgc
    20101 caaaagcacg gaatcgagcc acttgtgacg attacgcatt ttgattgtcc aatgcatttg
    20161 atcaaagaat acggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgt
    20221 cgagtcatct tcaatcgata taaaggactt gtcaaatact ggctgacatt caatgagatc
    20281 aatatgatcc ttcatgcacc attcatggga gcaggactgt actttgaaga aggagaaaac
    20341 gaagaacaag tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg
    20401 aagattgctc atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca
    20461 tattatccat acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa
    20521 aattatttct tcattgatgt tcagtcacga ggagaatacc cagcttatgc attgaaagaa
    20581 atggaacgaa atggcattca gatcgaaatg gaaccagggg atgccgcatt gctgaaagaa
    20641 catactgttg attttatctc tttttcttat tattcttccc gagtttcgac taccgacaac
    20701 gaactcttgg aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctgcaa
    20761 gcaagtgaat gggggtggca aattgatcca ttaggcttga gaataacgat gaatgatatt
    20821 tatgatcgtt atcaaaaacc attatttatt gtagaaaacg gcttaggtgc agtcgatacg
    20881 ccagatgaga atggttatgt ggaagatgat taccgaatcg attacctggc agcgcatatc
    20941 caggcgatga aggatgcagt agaactagac ggagtcgatc tattaggcta tacgacttgg
    21001 ggatgtattg atttagtatc agctggtacc ggtgagatga agaaaagata cggattcatt
    21061 tatgtcgatc gtgataacca aggaaatggc acattagccc gttcaaagaa aaaatcattc
    21121 aactggtata aaaaagtaat tgattcaaat ggagaaaatc tttcgaacga ttgatcaatg
    21181 atttataaaa gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa
    21241 acgcaagcag aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa
    21301 gtaaaggaga atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg
    21361 actgctgctt tatatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggtgcc
    21421 cctggcggcc aaatgaacaa tacggcagaa atcgaaaatt atcctggatt cgattcgatc
    21481 atgggccccg atctagctta taagatgtat gaaagtgtct caagatttgg gacagaaaat
    21541 gcttatggaa tcgttcaaga tatcaaggat cacggcagcc ataaagaagt catctgtgaa
    21601 gataaaagtt acgaaggaaa aacagtcatt atcgcaacag gctgtatcca ccgtaaatta
    21661 ggcgtacctg gcgaggaaga atttgctgga cgaggcgttt cttactgtgc agtctgtgat
    21721 ggtgcgtttt tccgtaacaa acgtctagta gtcatcgggg gtggggactc agcagtagaa
    21781 gaagccatct atttgacaca atttgcttct gaagtagtga tcgtccaccg tagagatgaa
    21841 ttacgtgcac aaaagatcat ccaagaccgt gcatttgcaa atgaaaaaat ttcctttatg
    21901 tgggatacag tcgttgacga aatcgttgga aacgaaatgg tagtgacagg tgtgaaagca
    21961 cgtaatgtca aaaccggcaa tgaatttgat ttagcagcag acggagtatt catctatgtt
    22021 ggactggatc cattaactgc accttttaga gaagcaggtt tgacgaacga agctggctgg
    22081 attccaacag acaacgaaat gaaaacgaat atgccaggcg tttttgccat cggagatgtt
    22141 cgtgaaaaag atttgcgaca aatcactaca gctgttggtg acggcggtat tgctggacaa
    22201 caagtgtata aatacttgga atctttagaa gaaacaactg cataagttta aaaagagtag
    22261 aagaacgggt tcaattgttc ttttactctt ttttttcgcg tgaaatttac tgaatctgat
    22321 ttatgcgaac acgtccaaca ccttttgata aaagaatcct ttcttgattt aagcgttttt
    22381 tggtcctcat actttcatat ctaataagtg aatgctatac ttattaagaa ccatatttca
    22441 gaaagaggtg ttattttcat gtcttgggaa caagtgtatg aacaatgggt acatgaagaa
    22501 aatttagaag aaaacttaaa aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt
    22561 gaagatgctt tttatgcacc gttggaattc ggtactgcag gcatgcgggg aatcttagga
    22621 cctgggatca atcgaatgaa catctatacg gtaagacaag caacagaagg actagcacgt
    22681 ttcatggata cacaaggtga agaagcaaaa cgccgaggtg tcgctattgc ttatgactca
    22741 cgccatcagt caccagaatt tgcaatggaa gcagccaaaa cactggcaca tcacaatatc
    22801 ccgtcttatg tattcgaaag tctgcgtcca acaccagagc tttcttttgc agttcgtcat
    22861 ttgaagacgt ttgcgggaat catggtaact gcatctcaca acccagcggc ttacaatgga
    22921 tataaagtct acggagaaga tggcggacaa atgcctcctg cagacgcaga tgcattgacg
    22981 aaatatgtcc gtgaagtcag caatccgcta aaagtcgaag tattatctga cgaagaagct
    23041 aaacatagcg ggttgatcac gatcatcgga gaagaagtgg acgctgctta tctagaagaa
    23101 atcaaagcag tgacgattga tcgtgaattg gtagaaacaa tggggaaaga tttgaaactt
    23161 gtgtacacgc cacttcatgg aacagggaaa atgttgggcg aacgtgcgtt gaaacaagct
    23221 ggattcgaac aattcgtact agttcccgaa caagctgtgg cagatccaga tttcactacg
    23281 gtcaaatcgc caaatccaga agaacattca gcttttgaat atgctatacg acttggagaa
    23341 aaagaaggag ctgacttgct gattgcaaca gatcctgatg cagaccgttt aggcgcagcg
    23401 gttcgtttgc cagatggttc ttatgaagta ttgactggga accaaatcgg ggcattgttt
    23461 gctcaatata ttttggaagc acatcaatca agaggcactt taccagaaaa cgcagcggta
    23521 ttgaaatcaa tcgtttccag cgaattgcct acagcaattg cggaacacta cggtgctacc
    23581 atgttcaatg tgttgacagg gtttaaattc attgctgaaa aaatccaaca attcgaagaa
    23641 gaccattcac atacttttat gtttggattc gaagaaagtt atggttattt agttaaacct
    23701 tttgttcgcg ataaagacgc gatccaagca ttgcttttat tagcagaagt agcagcttac
    23761 tataaaaaac aaggcaaaac gttgtatgac ggcttgcaaa cgatttttga aacctatggc
    23821 tattttgcag aaaaaacgat ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc
    23881 tctgctttga tggaaaaatt ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt
    23941 attcaaacag aagactttaa agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa
    24001 ctaacaacac cgccatccga cgtattgaaa tatacattgg aagaaaatgg ctggattgct
    24061 atccgccctt caggaactga gccgaaaatc aagttttata ttggtgtaaa aggaagctct
    24121 acagaagacg cagccgaaaa agtagcctct tatgaagcga ttatcaaaaa gatgacagaa
    24181 gaataaaaaa cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca
    24241 agttggaggt gaatggattg ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat
    24301 tattcaaaat gttgagtgat cccactcgtc ttaaaattct gctatatctg aaagacgggg
    24361 aacaaaatgt gacagcaatc acccaagcag tggaaatgga gcagtctgct gtatcgcatc
    24421 aactacgttt attaagagaa aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt
    24481 tgtatagtct tgatgattct catgtactgg atattttgaa ccagacactc aaacatgtgg
    24541 aacatcaata acgataggtg caaatagata aacactggag tgtgacaacg aaatttcttc
    24601 gttgtcacac tccagtgttt tttaagaaaa agacaaaaat gattaatcgc ttttttctgt
    24661 tgtttcagtt ccataataac tatgatcttc taggtctaaa gcattcaaaa taagcgaagc
    24721 cgtttcttca atagataaag atgccacatt gatcactaca catcctagtt tttcataaag
    24781 atcattcgca aaatcgagtt cagctctaat tttttcgata tcggaataag cagtatcagg
    24841 gtttaatccg taagagcgca tccgttcttt acggatatta tttaaaatat ctgggttatt
    24901 cgttagtccc acgatttttt taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg
    24961 tgggatcaaa ggtaaattgg caacttttaa atttttgtta gctaaaaaca agctcaaagg
    25021 cgttttggaa gttcgggata cgcctagcaa aacgacatct gcttctaaaa aaccacgagg
    25081 atctttccca tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt
    25141 ctcattcaat aaatggaggg caccaggttg ccgaattggc ttgataccgg ttaaacgttc
    25201 aatttctgat atcggtggcg tcatcacatc aaaactaaat aattgatgtt cttcacaaaa
    25261 agaacgtgtt atctcgatca gttcttgatt gattaatgta tgcaggacaa gtgctttttc
    25321 ttctaatgca tcttgcaatg cctgctttaa tgttttttca tctgtcacga acgttctgcg
    25381 gaataggttg aactcaacgg taggatactg agccatagta gcttgcgcta gttttgtcgc
    25441 agtttctcca gcagagtcgg agatgacaaa aaaagtaacg atttctgtag aatcggaaga
    25501 catggataaa acctcacttg catttttttt atgtttatta taccatactc tagaatgcgt
    25561 acttcgtaat cattttgatt tggaggaata agtcatgaaa aaacagacat tgatcgaaca
    25621 atcattagaa acattgaaaa ataacggttt taaatatact aaaaaaagag aagcattgtt
    25681 aacttattta gtgaagcgta accgctacgt atctgctaaa gaagtttttg actatatgaa
    25741 tcaggaattc aaaggggtaa gttatgatac gatttatcgg aatctccatg attttgcaga
    25801 gatcgattta ctggaagaaa ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg
    25861 tcagggggaa atcggccatc accatcattt tatctgtaca gtgtgcggaa caacgaaaga
    25921 aatccatatg tgtccgatga attatttcga agaacagcta ggtggatgca cgatcgaagg
    25981 gcatagattc gagattttcg gaagatgtga aaattgtcaa taagggttta aataaaataa
    26041 catttgataa ttttaaaaaa aagcaagatt tccttgacgg tcaaaacagc attagatata
    26101 atatttacag gacagtcttg ttttttgaga tatatttcaa taacaattac ggttactttt
    26161 taagctcgga gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt
    26221 gacgatgctc ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt acaagaatct
    26281 cgtaaacgtg aattctacga aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct
    26341 agaaaacgca aaaaattcta gttctgtgat gaattggagt tggtagtgtg tcactactaa
    26401 gtagattaaa cgatgacatg aaagcagcga tgaaagcgaa agataaggaa tctttacaag
    26461 tcatccgaat gatcaagtca tctatacaaa acgagcagat taaagaaggt cacgacttga
    26521 cagaagaaga agaactaacg gtgttgtctc gcgaaatgaa acagcgacgt gattctttgc
    26581 atgagtttga agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa
    26641 tagtcgaaaa atatatgccg gaacaacttt ccgatgaaga aattcgacaa cttgttcaag
    26701 aagcaatcac tcaaactggt gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa
    26761 ttatgcctaa agtcaaagga aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac
    26821 tattacaatc ttaattttat catttaaact gcggacagtg attgtccgca gttttttgtg
    26881 ttcgtaaaaa aagaactaaa aa
//
LOCUS       DACCCM010000034        26833 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCCM010000034 DACCCM010000000
VERSION     DACCCM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.035"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(718..3867)
                     /locus_tag="IVK78_001431"
     CDS             complement(718..3867)
                     /locus_tag="IVK78_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6374911.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3881..5125)
                     /locus_tag="IVK78_001432"
     CDS             complement(3881..5125)
                     /locus_tag="IVK78_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP6374912.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5118..6710)
                     /locus_tag="IVK78_001433"
     CDS             complement(5118..6710)
                     /locus_tag="IVK78_001433"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6374913.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7219..7944)
                     /locus_tag="IVK78_001434"
     CDS             complement(7219..7944)
                     /locus_tag="IVK78_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374914.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8098..8424)
                     /locus_tag="IVK78_001435"
     CDS             complement(8098..8424)
                     /locus_tag="IVK78_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374915.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8461..8826)
                     /locus_tag="IVK78_001436"
     CDS             complement(8461..8826)
                     /locus_tag="IVK78_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP6374916.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8863..9078)
                     /locus_tag="IVK78_001437"
     CDS             complement(8863..9078)
                     /locus_tag="IVK78_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6374917.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9207..9542
                     /locus_tag="IVK78_001438"
     CDS             9207..9542
                     /locus_tag="IVK78_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6374918.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9618..11615)
                     /locus_tag="IVK78_001439"
     CDS             complement(9618..11615)
                     /locus_tag="IVK78_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374919.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11713..11988)
                     /locus_tag="IVK78_001440"
     CDS             complement(11713..11988)
                     /locus_tag="IVK78_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6374920.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12220..12954
                     /locus_tag="IVK78_001441"
     CDS             12220..12954
                     /locus_tag="IVK78_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6374921.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12993..14135
                     /locus_tag="IVK78_001442"
     CDS             12993..14135
                     /locus_tag="IVK78_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6374922.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14230..15621)
                     /gene="sufB"
                     /locus_tag="IVK78_001443"
     CDS             complement(14230..15621)
                     /gene="sufB"
                     /locus_tag="IVK78_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP6374923.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15626..16096)
                     /locus_tag="IVK78_001444"
     CDS             complement(15626..16096)
                     /locus_tag="IVK78_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP6374924.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(16083..17318)
                     /locus_tag="IVK78_001445"
     CDS             complement(16083..17318)
                     /locus_tag="IVK78_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6374925.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(17315..18601)
                     /gene="sufD"
                     /locus_tag="IVK78_001446"
     CDS             complement(17315..18601)
                     /gene="sufD"
                     /locus_tag="IVK78_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP6374926.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18614..19387)
                     /gene="sufC"
                     /locus_tag="IVK78_001447"
     CDS             complement(18614..19387)
                     /gene="sufC"
                     /locus_tag="IVK78_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP6374927.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19584..20432)
                     /locus_tag="IVK78_001448"
     CDS             complement(19584..20432)
                     /locus_tag="IVK78_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6374928.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20456..21142)
                     /locus_tag="IVK78_001449"
     CDS             complement(20456..21142)
                     /locus_tag="IVK78_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374929.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(21135..22169)
                     /locus_tag="IVK78_001450"
     CDS             complement(21135..22169)
                     /locus_tag="IVK78_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374930.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(22564..22902)
                     /locus_tag="IVK78_001451"
     CDS             complement(22564..22902)
                     /locus_tag="IVK78_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP6374931.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22904..23260)
                     /locus_tag="IVK78_001452"
     CDS             complement(22904..23260)
                     /locus_tag="IVK78_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP6374932.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(23266..24453)
                     /locus_tag="IVK78_001453"
     CDS             complement(23266..24453)
                     /locus_tag="IVK78_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6374933.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24644..25489)
                     /locus_tag="IVK78_001454"
     CDS             complement(24644..25489)
                     /locus_tag="IVK78_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6374934.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25723..26688
                     /locus_tag="IVK78_001455"
     CDS             25723..26688
                     /locus_tag="IVK78_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP6374935.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7689 a         5549 c         4515 g         9080 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtattat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccctcc ctaaacaaac atcttttgta
     3901 agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtgt
     3961 cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc ctagtaaaag
     4021 gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt ccttcagtgt
     4081 tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg aactcattat
     4141 ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc gtcataccaa
     4201 gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg aattcatttg
     4261 gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca tcaaactgtt
     4321 ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag tctaaatctt
     4381 tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt tcccaatctt
     4441 ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg aacatttttt
     4501 gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga agagcgatag
     4561 tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat attggaaatt
     4621 gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct gaacctggat
     4681 tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata aaatattctg
     4741 aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat acacccggtt
     4801 ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc atgataaaat
     4861 cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta attttatatc
     4921 ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa ttgctattag
     4981 atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact ccatcttttt
     5041 tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct gggaaacgaa
     5101 tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg taaccacgct
     5161 tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt ttctagcact
     5221 tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc aattggttct
     5281 tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt ttctttgatt
     5341 tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc ataggtttcg
     5401 aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt gacaaattct
     5461 cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa tacaatcacc
     5521 gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac agcataaata
     5581 ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg gaataaaact
     5641 ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa accatgaaga
     5701 agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt aaaccgagaa
     5761 tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt cattactact
     5821 gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc cccattgcgg
     5881 agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc tagattgtat
     5941 gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt taagtagtta
     6001 cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact aaagaatcgt
     6061 cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat atgaggcgtg
     6121 taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag aaattcataa
     6181 gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa tttcttaatg
     6241 acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt tgattggaga
     6301 tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg cacaaaggct
     6361 ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa gacgttgaaa
     6421 agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat cagatcttct
     6481 tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct taccgtatca
     6541 tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg gtcggataaa
     6601 tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc cattttgcta
     6661 cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa aaagatacac
     6721 tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc ccttttagaa
     6781 aaagcaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa aggagtctaa
     6841 cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct acatagttat
     6901 ctttagaacc atttcagata catatgaaac aattacatat gattttttca taaaaaaaca
     6961 gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact aaaatcacta
     7021 cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc cgtttcattt
     7081 cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc agtagcttat
     7141 cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg taaataaatg
     7201 aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt aatcagttac
     7261 ctgtccgttc ttaaatacca cagaataatc ttttttccca ttttcaagta accgaagtgg
     7321 aatatcttta ccaactttct ctgtcactgc attactgata cctgttagat tagctaataa
     7381 atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg tgattccttt
     7441 atcttcattt acaagtttcc aagtaggtgg tatagctgtg tatgtttcta caaatgaatt
     7501 aagtttttca ttaagtgact tctcttttat atttccatct aaaaactctg tatatatggc
     7561 ttgagctata ctaaaacaaa gctggccttg ttctaataat tttacatcat tagttagtaa
     7621 agctgtgttc acgttatcaa tcgcttcaag aagagtatct ttggtgtccc taacagcttt
     7681 aggtgtgcct tctggaattt ccctacttga tacctcgtcc attttatctt ccataaaccc
     7741 cttaaaatca tatctttctc ccaaatccat agaagttttt gagaaagctt tcatttctga
     7801 gtctatttct tcaataatat ttaacatgtc tgatacatat tgtttaggct gattcgtttt
     7861 taatctatat gtggtaaata tctcaattcc tccaattatc aataaaattg ccaaacaaat
     7921 ccataataat tttttctttt tcatttttcc tcctatacac tgctgttata acagtgacta
     7981 atgtaaagat tttcccagca atcatcagac tattatttac agggaatcaa aaaaactcct
     8041 tagtaagctt acacctttag gagttcctgt ttgaacggta tgcataatac atttaaatca
     8101 atttaacatt aacacctcag tatatggtat ctctacatat acacggtctt tttttggata
     8161 gtactcgaca tttatattga tagcacttcc attgtctaac gtagctttag cccggtacgt
     8221 gttatcacca ttatcatgag tgatcgtcac atcatcacat gtccgattta agttatactg
     8281 ttcttgtaaa atctgagtta ccaatggtac agatgacttc tctagtccag atgaacccgt
     8341 cattcttgaa aaaattatac ctataatagc caatacaacg atacctataa tgacaaactt
     8401 cttattcata tttactttat tcaacttttt atctcctatc tccaaaatga ttttctctca
     8461 tcagtaactc attgacatcc ccaatagtac accaaagatg gctccagcta cccctgctat
     8521 catcaaaatc gtacccattt gttcatcttt ctctcgatat aagtaaccca taataacagc
     8581 aacagcacca aataccacag gaataaatgc taatgatact accatagaaa tccaaccaat
     8641 aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca tttcattaat
     8701 tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa
     8761 tgctccgcaa aactggcaat acttactttc atttgttatt tctttaccac attttctaca
     8821 atacatgtct tctcctccta aaatcattca attgcttatt agctaactgt ttactttatt
     8881 aaatacatca gaaaacagct catttattgg tacacttaat atctcagaaa gcattttcat
     8941 ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt gctgtgtaac
     9001 attcattttt ttggctaact cagtttggga aagtcctttt tcttttctgt atcggctgag
     9061 attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca atatgatgta
     9121 tttcaactca taaaatatat aaatacttca aatgtcatta caaatacaac aacttgttgt
     9181 actctactga gaaggggggt gtattaatga aacagaagca atatgcaaag gtcattagag
     9241 aaaagcgaaa agcactcggg tggacacaaa aagaattagc taaaaaaatc ttctctacac
     9301 aacaggcagt agcaagatgg gaaaattctg taacagaacc taacctcgat agcttaacag
     9361 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gacatagatt
     9421 atgaggaaga gtttttggct ctctatcgct ctcttagtac tgaagatgca attcgaacaa
     9481 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
     9541 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
     9601 agctatggat aacctgtcta gtttaatata attgggtctc ttctattcct tactctccgt
     9661 ccatcatctc ttgttacact tgtaaattga taaataggaa ctaattcaac tcctgataat
     9721 atcaattctt ttattacttc atctatccct gcccctttcg ctagatatac taaaaaacta
     9781 ctgcttgctt tcacattgta tagtttctta atatccttag aagctcgttc aagagtgaat
     9841 ccttgtttaa caaagtagcc tctgttttct attaaagcat ttttcacgct gttatccctg
     9901 acatttcgta ggcgttcaca aacagtgtta ataagttctt tttctgtcat tagatatgtc
     9961 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
    10021 ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
    10081 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt
    10141 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
    10201 tagacattgc ttctcttatg tttaaagttt cctaacgctt tctcttcctt taacctattt
    10261 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
    10321 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
    10381 gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
    10441 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
    10501 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
    10561 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
    10621 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
    10681 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
    10741 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
    10801 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
    10861 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
    10921 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
    10981 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
    11041 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
    11101 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
    11161 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
    11221 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
    11281 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
    11341 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
    11401 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
    11461 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
    11521 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttcttgatct
    11581 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
    11641 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
    11701 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
    11761 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
    11821 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
    11881 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
    11941 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    12001 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    12061 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    12121 acataatacc tcgataaaaa aggggtgtca acagctttgt aaaatttatt tacaacaccc
    12181 cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    12241 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    12301 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    12361 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    12421 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    12481 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    12541 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    12601 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    12661 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    12721 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    12781 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    12841 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    12901 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    12961 gtactccttg taaactaaga ggagatgatt taatggcaaa aatatcaaag tacaaaaaga
    13021 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    13081 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    13141 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    13201 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    13261 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    13321 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    13381 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    13441 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    13501 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    13561 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    13621 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    13681 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    13741 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    13801 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    13861 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    13921 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    13981 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    14041 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    14101 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    14161 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    14221 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    14281 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    14341 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    14401 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    14461 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    14521 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    14581 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    14641 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    14701 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    14761 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    14821 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    14881 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    14941 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    15001 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    15061 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    15121 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    15181 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    15241 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    15301 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    15361 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    15421 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    15481 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    15541 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    15601 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    15661 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    15721 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    15781 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    15841 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    15901 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    15961 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    16021 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    16081 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    16141 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    16201 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    16261 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    16321 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    16381 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    16441 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    16501 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    16561 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    16621 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    16681 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    16741 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    16801 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    16861 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    16921 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    16981 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    17041 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    17101 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    17161 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    17221 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    17281 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    17341 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    17401 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    17461 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    17521 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    17581 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    17641 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    17701 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    17761 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    17821 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    17881 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    17941 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    18001 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    18061 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    18121 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    18181 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    18241 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    18301 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    18361 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    18421 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    18481 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    18541 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    18601 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    18661 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    18721 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    18781 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    18841 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    18901 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    18961 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    19021 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    19081 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    19141 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    19201 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    19261 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    19321 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    19381 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    19441 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    19501 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    19561 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    19621 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    19681 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    19741 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    19801 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    19861 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    19921 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    19981 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    20041 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    20101 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    20161 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    20221 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    20281 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    20341 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    20401 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    20461 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    20521 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    20581 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    20641 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    20701 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    20761 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    20821 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    20881 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    20941 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    21001 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    21061 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    21121 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    21181 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    21241 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    21301 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    21361 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    21421 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    21481 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    21541 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    21601 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    21661 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    21721 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    21781 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    21841 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    21901 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    21961 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    22021 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    22081 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    22141 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta tgatttttca
    22201 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    22261 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    22321 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    22381 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    22441 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    22501 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    22561 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    22621 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    22681 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    22741 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    22801 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    22861 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    22921 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    22981 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    23041 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    23101 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    23161 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    23221 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    23281 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    23341 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    23401 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    23461 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    23521 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    23581 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    23641 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    23701 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    23761 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    23821 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    23881 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    23941 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    24001 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    24061 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    24121 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    24181 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    24241 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    24301 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    24361 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    24421 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    24481 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    24541 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    24601 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    24661 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    24721 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    24781 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    24841 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    24901 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    24961 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    25021 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    25081 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    25141 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    25201 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    25261 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    25321 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    25381 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    25441 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    25501 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    25561 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    25621 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    25681 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    25741 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    25801 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    25861 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    25921 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    25981 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    26041 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    26101 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    26161 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    26221 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    26281 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    26341 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    26401 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    26461 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    26521 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    26581 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    26641 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    26701 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    26761 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    26821 caagaggtcg taa
//
LOCUS       DACCCM010000035        26686 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCCM010000035 DACCCM010000000
VERSION     DACCCM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.036"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(106..1053)
                     /locus_tag="IVK78_001456"
     CDS             complement(106..1053)
                     /locus_tag="IVK78_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6374936.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IVK78_001457"
     CDS             1299..1763
                     /locus_tag="IVK78_001457"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP6374937.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IVK78_001458"
     CDS             complement(1825..2868)
                     /locus_tag="IVK78_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP6374938.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            3219..4082
                     /locus_tag="IVK78_001459"
     CDS             3219..4082
                     /locus_tag="IVK78_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6374939.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(4211..5833)
                     /locus_tag="IVK78_001460"
     CDS             complement(4211..5833)
                     /locus_tag="IVK78_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6374940.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            6082..6603
                     /locus_tag="IVK78_001461"
     CDS             6082..6603
                     /locus_tag="IVK78_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374941.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(6789..8519)
                     /gene="spxB"
                     /locus_tag="IVK78_001462"
     CDS             complement(6789..8519)
                     /gene="spxB"
                     /locus_tag="IVK78_001462"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP6374942.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            8684..9502
                     /locus_tag="IVK78_001463"
     CDS             8684..9502
                     /locus_tag="IVK78_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP6374943.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(9605..9802)
                     /locus_tag="IVK78_001464"
     CDS             complement(9605..9802)
                     /locus_tag="IVK78_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374944.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            9971..10549
                     /locus_tag="IVK78_001465"
     CDS             9971..10549
                     /locus_tag="IVK78_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6374945.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(11594..12097)
                     /locus_tag="IVK78_001466"
     CDS             complement(11594..12097)
                     /locus_tag="IVK78_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6374946.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            12456..13481
                     /locus_tag="IVK78_001467"
     CDS             12456..13481
                     /locus_tag="IVK78_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6374947.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            13828..14706
                     /locus_tag="IVK78_001468"
     CDS             13828..14706
                     /locus_tag="IVK78_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374948.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            15182..15298
                     /locus_tag="IVK78_001469"
     CDS             15182..15298
                     /locus_tag="IVK78_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6374949.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            16226..16564
                     /locus_tag="IVK78_001470"
     CDS             16226..16564
                     /locus_tag="IVK78_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374950.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            16658..16888
                     /locus_tag="IVK78_001471"
     CDS             16658..16888
                     /locus_tag="IVK78_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374951.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            17288..17659
                     /locus_tag="IVK78_001472"
     CDS             17288..17659
                     /locus_tag="IVK78_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP6374952.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(17933..18625)
                     /locus_tag="IVK78_001473"
     CDS             complement(17933..18625)
                     /locus_tag="IVK78_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6374953.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(18628..19413)
                     /locus_tag="IVK78_001474"
     CDS             complement(18628..19413)
                     /locus_tag="IVK78_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6374954.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            19650..21050
                     /gene="lacG"
                     /locus_tag="IVK78_001475"
     CDS             19650..21050
                     /gene="lacG"
                     /locus_tag="IVK78_001475"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6374955.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21075..21392
                     /locus_tag="IVK78_001476"
     CDS             21075..21392
                     /locus_tag="IVK78_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6374956.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            21405..23078
                     /locus_tag="IVK78_001477"
     CDS             21405..23078
                     /locus_tag="IVK78_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6374957.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            23092..24723
                     /locus_tag="IVK78_001478"
     CDS             23092..24723
                     /locus_tag="IVK78_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP6374958.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            25595..>26686
                     /locus_tag="IVK78_001479"
     CDS             25595..>26686
                     /locus_tag="IVK78_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP6374959.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSF"
BASE COUNT         8389 a         4596 c         5362 g         8339 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt
     3061 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt
     3121 agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga
     3181 cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt
     3241 cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga
     3301 atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc
     3361 gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc
     3421 gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt
     3481 tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg
     3541 aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag
     3601 ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc
     3661 tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga
     3721 aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga
     3781 gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc
     3841 gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac
     3901 cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa
     3961 aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa
     4021 cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat
     4081 aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact
     4141 cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct
     4201 aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa
     4261 attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc
     4321 ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa
     4381 atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat
     4441 catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt
     4501 tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc
     4561 ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca
     4621 accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag
     4681 aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga
     4741 taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt
     4801 ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta
     4861 cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg
     4921 gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac
     4981 gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga
     5041 gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt
     5101 gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta
     5161 gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca
     5221 cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac
     5281 cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata
     5341 acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag
     5401 ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct
     5461 cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt
     5521 tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga
     5581 ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc
     5641 gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt
     5701 taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg
     5761 taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat
     5821 ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa gtcttataga
     5881 attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga
     5941 aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta
     6001 gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa
     6061 gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt
     6121 ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta
     6181 gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt
     6241 gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg
     6301 atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca
     6361 ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct
     6421 gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta
     6481 ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc
     6541 tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga
     6601 taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg
     6661 gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa
     6721 atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag
     6781 cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca
     6841 tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga
     6901 tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct
     6961 gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta
     7021 tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat
     7081 ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag
     7141 acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata
     7201 gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg
     7261 gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt
     7321 tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg
     7381 acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga
     7441 atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt
     7501 tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt
     7561 tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat
     7621 tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca
     7681 aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg
     7741 ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt
     7801 ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt
     7861 tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca
     7921 agataaggca aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga
     7981 gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata
     8041 gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc
     8101 ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga
     8161 ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag
     8221 aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc
     8281 gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca
     8341 gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt
     8401 tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg
     8461 tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg
     8521 gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt
     8581 tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat
     8641 atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt
     8701 gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc
     8761 tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat
     8821 gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc
     8881 tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg
     8941 tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc
     9001 acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc
     9061 tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc
     9121 aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg
     9181 tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag
     9241 tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt
     9301 gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc
     9361 cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt
     9421 gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat
     9481 tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg
     9541 aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca
     9601 tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga
     9661 taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga
     9721 caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta
     9781 agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa
     9841 agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac
     9901 ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga
     9961 ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat
    10021 catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc
    10081 tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg
    10141 gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc
    10201 gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga
    10261 gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat
    10321 tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt
    10381 gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg
    10441 aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct
    10501 agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg
    10561 tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata
    10621 ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta
    10681 gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg
    10741 tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga
    10801 tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt
    10861 ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc
    10921 cacagtggat aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga
    10981 tttcttttga taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt
    11041 ataaaacggt gtggtttttg gaataaaaaa ataacatatc tcattttacg cttttgttat
    11101 tttatactta aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct
    11161 aaaaactact gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac
    11221 tggcagaatc aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta
    11281 attacttata aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa
    11341 gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt
    11401 taagctaaaa aaaaaacctt tttgttaaat aaggggaata tagcctgtca gtttcaaact
    11461 gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc
    11521 aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt
    11581 tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca
    11641 gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata
    11701 gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta
    11761 tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga
    11821 agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat
    11881 tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg
    11941 tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag
    12001 tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata
    12061 aataaatccc taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag
    12121 tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag
    12181 ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta
    12241 agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt
    12301 cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc
    12361 aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc
    12421 agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca
    12481 ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat
    12541 tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct
    12601 ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga
    12661 tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc
    12721 ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc
    12781 atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc
    12841 agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg
    12901 ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa
    12961 aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct
    13021 acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt
    13081 attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc
    13141 atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt
    13201 catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg
    13261 cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt
    13321 tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt
    13381 tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt
    13441 tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc
    13501 aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga
    13561 ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta
    13621 tatttattct atcaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta
    13681 actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt
    13741 cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg
    13801 gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg
    13861 atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc
    13921 gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat
    13981 agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc
    14041 tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg
    14101 aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct
    14161 tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt
    14221 ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa
    14281 atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct
    14341 cttcatattc cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt
    14401 gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat
    14461 acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt
    14521 tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg
    14581 atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga
    14641 caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact
    14701 tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt
    14761 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    14821 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
    14881 taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata
    14941 ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta
    15001 gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc
    15061 gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt
    15121 aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca
    15181 tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac
    15241 acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga
    15301 agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg
    15361 gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa
    15421 attggtagaa tctttctttt caaattgata ccattcccta attccagtta tatagttaac
    15481 tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc
    15541 ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc
    15601 gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga
    15661 aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg
    15721 tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt
    15781 tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa
    15841 agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat
    15901 caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta
    15961 gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt
    16021 aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag
    16081 tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga
    16141 taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc
    16201 tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa
    16261 cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc
    16321 attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta
    16381 cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac
    16441 accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac
    16501 tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat
    16561 gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca
    16621 caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga
    16681 aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt
    16741 caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat
    16801 agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt
    16861 tttggattat gttagtatgc aacagtaagg gagttcttat tatgaaaaat tttgacacat
    16921 attatgttat tgttttttcg atatctttgc tgttaatatt actaacttta atgcagtata
    16981 aagagtatcc agagtatttt attttaactt ttgtatattt agtaagtagc tacacacttc
    17041 tatataaatt cagaagttct tctagagttt ctaaagtttg tttcttaatt agcttattga
    17101 ttctataata tttttgattt atataggaaa ttaaaaatat tatagaatca acactcagag
    17161 aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc ctggttggga
    17221 aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga
    17281 taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc
    17341 attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat
    17401 tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg
    17461 catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac
    17521 ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat
    17581 ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga
    17641 actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac
    17701 tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa
    17761 ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg
    17821 ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta
    17881 taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat
    17941 taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat
    18001 ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa
    18061 taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa
    18121 caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt
    18181 gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg
    18241 ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg
    18301 ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact
    18361 gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca
    18421 atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat
    18481 tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat
    18541 catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt
    18601 tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc
    18661 agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg
    18721 atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt
    18781 gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg
    18841 tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact
    18901 gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa
    18961 tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg
    19021 agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa
    19081 atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg
    19141 aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt
    19201 ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac
    19261 acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga
    19321 aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc
    19381 tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat
    19441 ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt
    19501 ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt
    19561 tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa
    19621 actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg
    19681 gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg
    19741 ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt
    19801 tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa
    19861 gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag
    19921 gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg
    19981 tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc
    20041 gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag
    20101 tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag
    20161 gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata
    20221 tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa
    20281 ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg gaagataagc
    20341 atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct acctatttag
    20401 gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca aatcaggcca
    20461 gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg cgtaatgact
    20521 acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa
    20581 tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag ggaatcggag
    20641 aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt tatccagaag
    20701 gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac aaaatctata
    20761 tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt atggatcagc
    20821 cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata acagccgggg
    20881 taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg acaaatgggt
    20941 ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt tacccaaaag
    21001 aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa taagtgataa
    21061 tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag atcgttgcat
    21121 attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga caaggaaact
    21181 ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt
    21241 cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg ggattcattt
    21301 ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg attcaagact
    21361 ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat ggaatcatta
    21421 aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt tatttaggag
    21481 ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc attttcattt
    21541 tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc tccacatggc
    21601 tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca gcaaccactg
    21661 ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta atcaacacca
    21721 cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag
    21781 gtggattttc aacggaatat atggggacaa agggactact ggcttccttt gtcgctgcat
    21841 ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt aaaatgccta
    21901 aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc tctttttcag
    21961 tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc tcatttgcag
    22021 aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac ttaggaatcg
    22081 ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg
    22141 tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg caattgttca
    22201 attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt gtaggaacta
    22261 tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt gctaagtcta
    22321 agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct gtcaacgaac
    22381 cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca tttttgttaa
    22441 cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc atgaacagct
    22501 ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta ggaacgggca
    22561 tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc
    22621 taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa aaagcagagg
    22681 tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct gtggcagcga
    22741 gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag
    22801 ggacaagtgc gatgttagct aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga
    22861 tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat tttgatatgg
    22921 tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac accgatcgct
    22981 taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg agagatcctg
    23041 aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat aatgaaaatg
    23101 ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt gggggctgtt
    23161 tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat ttcgatcctg
    23221 tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa agatgcctta
    23281 gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca ggatccttat
    23341 aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac gattgacctt
    23401 gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta cagtgatttt
    23461 tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac ctttgaagaa
    23521 atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa gcagctggac
    23581 gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct ctggccttat
    23641 ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt tttgaagtct
    23701 ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct ccacttagct
    23761 gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag tcgaggtgtg
    23821 tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat gaatgaactt
    23881 caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt agttgaaaca
    23941 gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg tgaagccgaa
    24001 gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt gctggatctt
    24061 gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta ttgggaacct
    24121 ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta tctcagtgtt
    24181 caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc tggaaacgcc
    24241 ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt taaaaatgca
    24301 agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa
    24361 ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga ctacaaacta
    24421 actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag tggcataagc
    24481 aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga tactaatcag
    24541 ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa aacaagtgaa
    24601 tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga aattggggtt
    24661 tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa
    24721 tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat cgttagcgta
    24781 gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa cgatttccag
    24841 tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat tatgctaaag
    24901 agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg cagataacat
    24961 aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt tgagcactta
    25021 gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct ttttatgata
    25081 agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat cttattttta
    25141 atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc cccctccata
    25201 ttatgttggc tctttattcc cactctgttt tcaacgtaaa gtgtataaaa cagaatgcga
    25261 tactatcaag ggagttaaaa caatatacag aacagtaaaa cactataaat aaaaataaaa
    25321 gtgcaataaa agtgcaattt ttgcacctaa tcatactctg tttatatatg aaaactagat
    25381 tctgaatcca atatttggtt aacgagaggc gttcgaattt gaacgtcttt tatatattac
    25441 tttcaaataa atatgaggta gaatactatc tattttcttt ttaacccaat tgttataatg
    25501 gagagtgtaa aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt
    25561 accacaacac attcacagaa gagaggactt ccctatgaac gattttacta cagaaattct
    25621 aaagactcta gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggagaaagc
    25681 agtcaatacg cttctcaaaa cggagttaac ggctttccta gattacgaaa agtatgatcg
    25741 cattggtttt aacacgggta attctcgtaa cggctcctat gaccgtacgg tcaagaccga
    25801 gtatggggaa cttcatctcc agattccgcg cgaccgcaat ggtgagttca agcaacagac
    25861 tgttcctgct tatagacgga cgaatgacac gttagaggag accgtcattc acctcttccg
    25921 aaaaggtatt accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcattacta
    25981 cacgccccaa accatgtcca atataacaaa atcatttaca gaagaggtaa cggcgtttaa
    26041 tgggcgggag cttcatgacc gttatgctgc tatttatatg gacgcaacgt atattccgtt
    26101 aaagcggaaa accgtcgcca aggaagctat tcatatcgca gttggcattc gcccggacgg
    26161 atcaaaggaa gtattgagct atgcgattgc accgactgaa tccatcacga tttgggagga
    26221 aattttattg gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg
    26281 cttaaagggg atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg
    26341 ttgtgtacac gtttcccgta atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt
    26401 ctgtgatgat tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg
    26461 tggtactttt gcggaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc
    26521 gaacgatcac ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc
    26581 tacgaacttg attgaatcct ttaataaaca aatcaagaaa tacagccacc gcaaggaaca
    26641 gttccaaaat gaagagtcga tggaacgttt cttagtctcg tctttt
//
LOCUS       DACCCM010000036        26460 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCCM010000036 DACCCM010000000
VERSION     DACCCM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.037"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(99..1229)
                     /locus_tag="IVK78_001480"
     CDS             complement(99..1229)
                     /locus_tag="IVK78_001480"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP6374960.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVK78_001481"
     CDS             complement(1878..3080)
                     /locus_tag="IVK78_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6374961.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVK78_001482"
     CDS             complement(3140..3343)
                     /locus_tag="IVK78_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6374962.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVK78_001483"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVK78_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVK78_001484"
     CDS             4880..5692
                     /locus_tag="IVK78_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6374963.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="IVK78_001485"
     CDS             complement(6716..6913)
                     /locus_tag="IVK78_001485"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374964.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVK78_001486"
     CDS             complement(7059..7937)
                     /locus_tag="IVK78_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6374965.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVK78_001487"
     CDS             complement(7976..9094)
                     /locus_tag="IVK78_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP6374966.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVK78_001488"
     CDS             complement(9091..11190)
                     /locus_tag="IVK78_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP6374967.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVK78_001489"
     CDS             complement(11241..12062)
                     /locus_tag="IVK78_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6374968.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVK78_001490"
     CDS             complement(12059..12868)
                     /locus_tag="IVK78_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6374969.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVK78_001491"
     CDS             complement(12889..13383)
                     /locus_tag="IVK78_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6374970.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVK78_001492"
     CDS             complement(13395..13883)
                     /locus_tag="IVK78_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6374971.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IVK78_001493"
     CDS             complement(13960..16656)
                     /locus_tag="IVK78_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6374972.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRMTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IVK78_001494"
     CDS             complement(17084..18157)
                     /locus_tag="IVK78_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6374973.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IVK78_001495"
     CDS             complement(18154..18981)
                     /locus_tag="IVK78_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374974.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IVK78_001496"
     CDS             complement(18982..19788)
                     /locus_tag="IVK78_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6374975.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IVK78_001497"
     CDS             complement(19800..20885)
                     /locus_tag="IVK78_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6374976.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IVK78_001498"
     CDS             complement(20902..21444)
                     /locus_tag="IVK78_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6374977.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IVK78_001499"
     CDS             21702..22691
                     /locus_tag="IVK78_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP6374978.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IVK78_001500"
     CDS             22688..23425
                     /locus_tag="IVK78_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP6374979.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IVK78_001501"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IVK78_001501"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP6374980.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IVK78_001502"
     CDS             23989..24621
                     /locus_tag="IVK78_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6374981.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IVK78_001503"
     CDS             24706..25353
                     /locus_tag="IVK78_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6374982.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IVK78_001504"
                     /pseudo
     CDS             25358..26341
                     /locus_tag="IVK78_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
BASE COUNT         8103 a         5586 c         4483 g         8288 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagctgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt catccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactag cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
//
LOCUS       DACCCM010000037        26029 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCCM010000037 DACCCM010000000
VERSION     DACCCM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.038"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            592..1281
                     /locus_tag="IVK78_001505"
     CDS             592..1281
                     /locus_tag="IVK78_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6374983.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1268..2476
                     /locus_tag="IVK78_001506"
     CDS             1268..2476
                     /locus_tag="IVK78_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6374984.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2770..4041
                     /gene="serS"
                     /locus_tag="IVK78_001507"
     CDS             2770..4041
                     /gene="serS"
                     /locus_tag="IVK78_001507"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP6374985.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4286..4981)
                     /locus_tag="IVK78_001508"
     CDS             complement(4286..4981)
                     /locus_tag="IVK78_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP6374986.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(5071..6171)
                     /gene="ychF"
                     /locus_tag="IVK78_001509"
     CDS             complement(5071..6171)
                     /gene="ychF"
                     /locus_tag="IVK78_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP6374987.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(6180..6371)
                     /locus_tag="IVK78_001510"
     CDS             complement(6180..6371)
                     /locus_tag="IVK78_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP6374988.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(6392..7282)
                     /locus_tag="IVK78_001511"
     CDS             complement(6392..7282)
                     /locus_tag="IVK78_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP6374989.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(7269..8036)
                     /locus_tag="IVK78_001512"
     CDS             complement(7269..8036)
                     /locus_tag="IVK78_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6374990.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(8048..8761)
                     /gene="rsmG"
                     /locus_tag="IVK78_001513"
     CDS             complement(8048..8761)
                     /gene="rsmG"
                     /locus_tag="IVK78_001513"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP6374991.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8993..10894)
                     /gene="mnmG"
                     /locus_tag="IVK78_001514"
     CDS             complement(8993..10894)
                     /gene="mnmG"
                     /locus_tag="IVK78_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP6374992.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10911..12308)
                     /gene="mnmE"
                     /locus_tag="IVK78_001515"
     CDS             complement(10911..12308)
                     /gene="mnmE"
                     /locus_tag="IVK78_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP6374993.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12652..12990
                     /locus_tag="IVK78_001516"
     CDS             12652..12990
                     /locus_tag="IVK78_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6374994.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(13158..13916)
                     /locus_tag="IVK78_001517"
     CDS             complement(13158..13916)
                     /locus_tag="IVK78_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6374995.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13934..14752)
                     /locus_tag="IVK78_001518"
     CDS             complement(13934..14752)
                     /locus_tag="IVK78_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6374996.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14749..15105)
                     /gene="rnpA"
                     /locus_tag="IVK78_001519"
     CDS             complement(14749..15105)
                     /gene="rnpA"
                     /locus_tag="IVK78_001519"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP6374997.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(15223..15357)
                     /gene="rpmH"
                     /locus_tag="IVK78_001520"
     CDS             complement(15223..15357)
                     /gene="rpmH"
                     /locus_tag="IVK78_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP6374998.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15880..17214
                     /gene="dnaA"
                     /locus_tag="IVK78_001521"
     CDS             15880..17214
                     /gene="dnaA"
                     /locus_tag="IVK78_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP6374999.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            17412..18542
                     /gene="dnaN"
                     /locus_tag="IVK78_001522"
     CDS             17412..18542
                     /gene="dnaN"
                     /locus_tag="IVK78_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP6375000.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18771..19013
                     /gene="yaaA"
                     /locus_tag="IVK78_001523"
     CDS             18771..19013
                     /gene="yaaA"
                     /locus_tag="IVK78_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP6375001.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            19000..20124
                     /gene="recF"
                     /locus_tag="IVK78_001524"
     CDS             19000..20124
                     /gene="recF"
                     /locus_tag="IVK78_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP6375002.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            20121..22067
                     /gene="gyrB"
                     /locus_tag="IVK78_001525"
     CDS             20121..22067
                     /gene="gyrB"
                     /locus_tag="IVK78_001525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP6375003.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            22087..24558
                     /gene="gyrA"
                     /locus_tag="IVK78_001526"
     CDS             22087..24558
                     /gene="gyrA"
                     /locus_tag="IVK78_001526"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP6375004.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24794..25093
                     /locus_tag="IVK78_001527"
     CDS             24794..25093
                     /locus_tag="IVK78_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP6375005.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            25143..25655
                     /gene="ssb"
                     /locus_tag="IVK78_001528"
     CDS             25143..25655
                     /gene="ssb"
                     /locus_tag="IVK78_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6375006.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25681..25917
                     /gene="rpsR"
                     /locus_tag="IVK78_001529"
     CDS             25681..25917
                     /gene="rpsR"
                     /locus_tag="IVK78_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP6375007.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
BASE COUNT         7831 a         5110 c         5072 g         8016 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt tttaaaagtt ttttcgattt cgtttttcga aatcttccag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtca
      241 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
      301 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      361 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      421 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      481 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      541 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      601 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      661 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      721 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      781 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      841 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      901 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      961 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
     1021 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
     1081 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
     1141 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
     1201 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1261 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1321 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1381 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1441 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1501 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1561 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1621 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1681 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1741 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1801 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1861 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1921 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1981 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     2041 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     2101 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     2161 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     2221 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2281 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2341 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2401 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2461 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2521 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2581 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2641 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2701 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2761 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2821 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2881 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2941 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     3001 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     3061 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     3121 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     3181 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3241 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3301 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3361 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3421 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3481 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3541 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3601 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3661 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3721 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3781 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3841 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3901 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3961 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     4021 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     4081 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     4141 tggcttttga acaccgttta tcgtaaaaag ggactgtgac aagacttttg tcacagtccc
     4201 ttactattat ttaactatgg ataaacaaaa tgaggttctt tactacccct agatatctag
     4261 caaaatagct gatctttttg ctagtttatc ttcttgggac ggataaactt ccaataatcc
     4321 cgtatttttt cttcaagtag taacggattg ctaaagcagt tgccccgata atgataacta
     4381 ctactggatc taatactgga ttgataactg gtggcaatag tgttgtcgca gaaaacagta
     4441 cgacccaaag aagtgtcgtt ccacctagga taagcaatgt cttacccatt cctggtttct
     4501 tgcttcgatc tgctccggga tattcatatt gatagatgta tttgtacatc atataaaaga
     4561 cccagccacc gattgcagaa cctagaacga gtgtagaaaa gccataagct ggcgttgttc
     4621 cacgtgaaaa caatccggta atcgcaatca tgatacttaa tagaccaagc aacagcaacg
     4681 tattatctaa ccacatcaat aaaggggtgg atgaattttt tacttctgct ggcttattga
     4741 tgattgcttc cgttctttca gagactgtcc caaacaattg acgggctgtt cttccgcctt
     4801 tttgttctgc aactaaagta ggcagaatgt catgaagggc ttttactttt tcttcttcag
     4861 acaaattcgc tgcttctaaa gattttttca aatcaaaaat gtactgttga tttcttttgg
     4921 ttaattttgt ttctaaacta ttgttttctt ttacgattgc tcgtaattgt tctggttcca
     4981 ttatctaata gtcctcctta gccgttcctt ttctcaacgg tcgtcacaaa agacattcta
     5041 ccatattcta cagctagttt gatagccctt ttacacatta aagcggaata acattacgtc
     5101 gccgtcttgt acgacataat cttttccttc taatcgaaca cgtccagctt cttttgctgc
     5161 atgcatgttt ccgtaatgat ccaagtcttc aaaagagaca gtttctgcac ggataaatcc
     5221 gcgttcgaaa tctgaatgga tgattcctgc tgcttgtggt gctttgattc cttttttgaa
     5281 tgtccaagca cgtacttctt gttccccagc agtaaagtaa gtagctaagc ctaacaaatc
     5341 ataagcagca cggatcaact gatccaaacc tgattcttcg attcctaaag cttctaaaaa
     5401 ttccgctttg tcttcttcat ctaattctgc gatttcttct tctgcacgag cactgaccac
     5461 gatcacttct gcattttctt ctgcagcaaa gttgcgcact tcttgtacat aatgattcgc
     5521 gtctgcatct gctacgtcat cttctgagac attggctaca taaagaattg gttttgtcgt
     5581 caataaaaat agtgatttaa cgattttttg ttcttcttca gtaaattcga tcgttcgggc
     5641 agattttcct tcttccagta caggtttgat tttatccagt acagctagtt ctgccactgc
     5701 atctttatct ttggttttag caacttttgc tacacgagta tagcgtttgt tgatagattc
     5761 taagtctgct aacactagtt ccaagttgat cgtatcaata tcttctagtg gatcaacacg
     5821 tccttctacg tgagtaatat tatcatcatc aaaacagcga acaacgtgac aaatagcgtc
     5881 tacttggcgg atatggctta agaattgatt tcccaaacct tctcctttac ttgcgccttt
     5941 tacgattcca gcaatatctg taaattcaaa agtcgtcgga acagtttttt tcggatgtac
     6001 aagttctgtt aagcgttgca aacgccagtc aggtacttct accatcccta cgttggggtc
     6061 gatcgtcgca aagggatagt ttgctgcttc tgctcctgct tttgtgatcg cattaaacaa
     6121 agttgatttt cctacgttgg gcaacccaac aattccagca gtcaatgcca taaatttatt
     6181 cactctttct ctgcattttt ttcaattact ttttttaatt tcttttcaaa atctcgacgg
     6241 gtcatcatga cgatatgtcc gcaattcata catctgattt tgatatctgc gcccattcga
     6301 atgacttccc agcgattcgt ctggcaagcg tgaggttttt tcatttcaac gatatcgtgc
     6361 aagtcgtaca taaaaatagc ctcctttttg tttattcttc atcaaattga atattcaaga
     6421 tatcaagaat tcgagtcaaa tctttttgag aaagatattc aatctcgatc ttacctttcc
     6481 catttttttc ttggatagct acacttgtac caaatttatc catcaaacga tcttcgcttt
     6541 ctcggatata atatggtttt tcctttgcta gccttgggat ttttttcttt tcttttccat
     6601 cattttgatt taactcagaa acaagctgtt ccagttggcg gacagttaaa ttttcagtta
     6661 ctgcacgatt ggctaatttc aagatcaatg ttttattttt taaacctaac agcgtacggg
     6721 cttgtcccat agataaacgc tgatcttgta ccatttcctt gacaagatcc ggtaaagaaa
     6781 gcaagcgcaa ataattggcg atataaggtc tgctctttcc taaacgacta gctacttctg
     6841 cttgtgttag tttcaggttt ttcatcagca tctcatatgc ttctgcttct tctaacggat
     6901 tcagatcttc tcgctgcaag ttttccagca ctgctacttg catcatcgct tcttcgtcaa
     6961 agtctctaac gatagcaggg atcgtttctt tttcagctag tttggacgct cgaaaacgtc
     7021 gctcccctgc aataatctca tatcctttta ctgcggaacg gcgtacgatg atgggttgaa
     7081 agacacctga ttgttcaata gaattagcta gttcctgcaa agatacttct tcaaatgttt
     7141 tacggggctg atacggattt ggtcgcagct cattcaatgg tagctggaca acggtatcat
     7201 cttttacatc caccgtctct aagttttcta aatcttgaaa cagtgcatcg attcctcggc
     7261 ctaatccttt acttttcttc acgacttacc acttcctttg ccaatgcttg gtagacttca
     7321 gctcctcgag aacgtggatc gtagtcgatg atcggttttc catgactagg tgcttctgat
     7381 aaacgaatat ttcttggaat gatcgtttcg taaacttttt cgcggaagta tttacgtact
     7441 tcttctacga cttcatttcc cagatttgtt cgtgcatcat acattgtcag taagactccc
     7501 tcgatctcta attctggatt aaagtgtttt tggactaaac gaaccgtatt cagtaattgg
     7561 cttaatcctt ccaatgcgta atactcacac tgaacaggta tcaaaatcga atcacttgct
     7621 gtaaatgaat tgattgtcag atggccaaga gaaggcgggc aatcgatcaa aatataatca
     7681 tactgactgc ttacttctgc taaagatcct tttaatcttg attctcttgc catcattgac
     7741 gtcaattcga tctctgctcc tgctaattgg agagtcgcag gcacaatact caaattttcg
     7801 tgttcagtga tcaaagttgc ctcatcaatt ggcaattcat tgactagaac atcatagatg
     7861 tctcttgtca catcaggttt tctgatcccg acaccactgg tagcatttcc ttgtgcatcc
     7921 atatccacta gcaatacttt tttgccaagg ctagcaagac aagcacctag attcaccgtc
     7981 gtcgttgttt tgccgacgcc acctttttgg ttcgcaacag atattatttg tgccatctct
     8041 tctcctccta tttgattggc tgcttattag gcagtccggg ctttcttggg tatttctttg
     8101 gggttggttt ctttttttga ataacgatga tatgccgttg atcagagaca ttcggcaagg
     8161 taaccgcctc ttctttgagg aatttaccac ctaataaagc aatcgcttgt tttgcttctg
     8221 tcaattcctc ttcacttttt gctgctttca atgcataaaa atagccttct tgtttagcca
     8281 agggcaaaca caattccgct aacacattca aacgagcaac cgcacgagca gtgacaaggt
     8341 caaatgatgc acgaaagtga ggattttgcc caaatgtttc tgcccggtca tgatataagg
     8401 aagcatcgat tcctaataat cgggttaact cagataagaa ggttattcgt ttattcagag
     8461 aatcaacgat cgttatctgc agttctggaa aggcaatttt taaaggtatg ctaggaaacc
     8521 ctgcccccga accaacatcg cataaagata aaggttgcgt aaagtctact tcaaatgcta
     8581 atgtaagtga atcgtaaaaa tgcttcagat atacgtcttt ttcatccgta atagctgtaa
     8641 ggttcatctt ttcgttccat tcgactaatg tccgataata aatagcgaat tgattcattt
     8701 gatgatcggt aagctctatc cctttttctg ccaaggattg gcgaaattct tctggtatca
     8761 taaatattcc tttcattgtg aaacagcaat tgtttcacag attctctccc tactttaacg
     8821 caaaatctct agacatgcaa tccttccata tggattctta aaaatttgaa aagtaagaaa
     8881 agccgataga ctttttcttt ctagtatgac tcataaaata tacacagaag agagaactag
     8941 ctttgagcaa tttctgcaaa aactagttct ctcttctatt tattattacg tattacgctg
     9001 gttcactgac tttcgcaatc ttcccttgtt cgatatagac cattaggata cttaggtctg
     9061 cagggtttac gccactgatt cggctagctt gggcaattgt ttctggttgg attttctgca
     9121 atttttgttt tgcttctgtt gctaatccgt tgattgctgc gtagtcgatg ttttctggaa
     9181 tctttttcgc ttccatgcgt tttaattttt ctactttttc catggctttt ttgatgtagc
     9241 cttcatattt gatttggatt tctacttgtt cgatttcttt tcgtgtcaat tcttcatttt
     9301 ctggaataaa ccgtagaagt tctgcataag taatctcagg acgtcttaaa aagtcgcttg
     9361 ctagtacacc atcttttaat ggtgccgcat taatcgttgc taaaaagtct tggacttctt
     9421 ctgttggttt gattcgtaca ttcatcaaac gtttgatttc ttgttcaact gcttcttttt
     9481 tcttcaaata attcttgtat tgatcttctt ttactaagcc tacacggtga cccatttctg
     9541 ttaatcgcaa atctgcatta tcatgacgta aaagcagacg gtattcagca cgtgaagtca
     9601 gcaaacgata aggttcgttc gttccttttg ttaccaaatc atcgatcatg acaccgatgt
     9661 aaccgtcact tcgtttgatc actaaaggtt cttttccctg aactttcaaa gctgcattga
     9721 taccggcaat cagcccttgt ccagccgctt cttcataacc gcttgtacca tttgtttgtc
     9781 cagctgtata aagattttca acgactttag tttctaatgt cggacggagc tggtgtggct
     9841 ctactacgtc atattcgata gcatatcctg tacgcatcat ttctactttt tctaatccag
     9901 caatagaatg aagcatgttg acttgaacat cctctggtaa agaagtagat aaaccttgaa
     9961 catagacttc ttccgtattt aagccttctg gttccaaaaa taattgatga cgtggtttgt
    10021 ctgcaaaacg cacgatctta tcttcgatgg atggacaata gcgagcgcct accccttcaa
    10081 cgatccctgt aaacattggg gcacgatgta agttgtctcg aataatttgg tgagtcgtct
    10141 cacctgtata ggtcagccag caagattcct gatctagttt ataggcacta tctggtgtgc
    10201 tgaagctaaa atgattcggc tctttgtcac caggctgctc ttctgtaaca gaatagtcga
    10261 tcgtacttga ttttactcga ggcggtgttc ctgttttgaa ccgttcgata gcaaagccaa
    10321 gttcttttag atgattcgct agtccaatag aaggttgaga gttattcgga cctgatgaat
    10381 atttcaattc accgataatg atttctccgc gaagtgctgt tccggctgta ataacaactg
    10441 ctttggcccg gtagctcgct cctgtggacg tcactactcc atgacaaaca ccatcttcga
    10501 cgatcagttc ttcgacgatt ccttgtctta gcgtcaaatt tggttctttt tcgatcgtat
    10561 gtttcatttc tgttgaataa gcatgtttgt ctgcttgtgc tcgcaatgct cggacagctg
    10621 ggccttttcc tgtgttcaac atacgcattt ggatataggt tttatcgata ttcttaccca
    10681 tttctccgcc taacgcatcg atttctcgaa cgacaacgcc tttagctggt ccgcctacag
    10741 aaggattaca tggcatgaat gctaccatgt ccaaatttat tgtcagtaat aatgtttttg
    10801 atcccatacg tgcagcagca agtgcagctt ctgaaccggc gtgtcctgcg ccaacaacga
    10861 tcacgtcata cgcatctgct tcataatgtt ccaatcgttt gttcctcctt ttattttcct
    10921 aaacaaaatt gactgaataa ttgagtgatt agctcatctt gtacactgtc gccgacaact
    10981 tctcctagat agtcccagca acgcgtcata tctatttgaa cgagatccac aggcattcct
    11041 gaatcaatac ctgcaatgac ttcttcaagt gagagggaag ctttttctaa caacgcgata
    11101 tggcgggtat tagaaacata agtagcgtct ctttctcctg tttctccgct gaagaataaa
    11161 tcagaaatag ctgattccaa acgatctagt ccgtcgtttt tagctacgga aattgaaaaa
    11221 actggttcat tttcgatcaa tttcttcaat ttttcttgtt ccagctgtgc tggcaaatct
    11281 gttttattta gaagaatgat ccgtttcaat ccacttgtcg cttccaataa ttgttcatct
    11341 tctgctgtca gtggttcgct ttggtttaga acaagtaaga tcaagtctgc ttctgctaat
    11401 gcttttcggc ttctttcgac cccgatccgt tccaccacat cttctgtttc ccgaattcca
    11461 gctgtatcga ttagcttgag cggtaccccc cgaacattga catactcttc gattacatct
    11521 cgagtcgttc ctgcaatatc cgtaacgatt gctttttctt ctcttaacaa atggtttaac
    11581 aaacttgatt tgccgacatt tggccggccg atgattgccg tacttaaccc ttcccgcaga
    11641 accttccctt gcttggatgt agcaagtagc gcttggattc tttgttggat catctgcgct
    11701 ttttcaagca ataacttcgt agtcaattct tcgacatcat catattctgg ataatcaata
    11761 tttacttcta cttgtgctaa tgtctctaaa atctcttgtc tcaatgagcg gatcaaagca
    11821 gacagattgc cgtctaactg attaagagct agtcccattg ctttgtctgt ttttgcccga
    11881 atcaaatcca tcacagcttc tgcctgtgaa aggtcaacac gaccattcaa gaatgctcgt
    11941 tttgtgaatt cgcctggctc agctaatctt gcaccttctc ttaacaatag ctgcaaaatt
    12001 tgattgacta caacgatccc accgtgacaa ttgatttcta ctacatcttc ccgcgtaaat
    12061 gtctttggtg ctctcatcac ggagaccatg acttcatcga caagctgatt gctcttggga
    12121 tcaacgatat gtccataatg gatcgtatga cttggtactt ctgataatcg tttgtttcca
    12181 gattgataaa ctttatctgc tagttgcaca gcttgatcgc cacttaaacg aacgatactg
    12241 attgcacctt ctccaggtgg ggtagaaatt gccgcaatcg tatcaaattc taaagtaatt
    12301 tcagccattt tttcacttcc tttttcattt tgcgcacaaa aaaagtgccc actccaccct
    12361 ttatgcacaa taaacggtga acttcgcact ttgactgctt gttcttgatt aaattctggt
    12421 catagattat cacacttgct ataaggaatc aagtgtttaa aacaattaga tgaagttgag
    12481 cctgttctgc ttccattacc ttatatttaa ttgtccttgg atgtctcttt gcgttctttg
    12541 gtttattcga agagaagata ctgtataaac aaggctttat ctttcttaaa aaaaacggta
    12601 cactaaatgg tagcgctatc taaactatac acttttgaaa ggagaaaata tgtgagcccg
    12661 ttcagatctg ctgcattgga agaagaaagt gaccctcgga tcaacgatgt cgttttatct
    12721 acagggaata tcaaccagaa atatatcgtc cgcgatatcg tttttgttgt ggaatgctta
    12781 gaagctgatt tgtttgaccc atcttttccc aaagatgacc ttttcttaac aacgaagagg
    12841 aaattaaaga cgaaggtact tgaatatggt gcgaatgcag taatcaactg tcattttgag
    12901 tatcagcaaa aaaaacgcga cggaaagtat tttctggaac tttttgctta tggaacagtg
    12961 atccaattta tccaaacaac gattggttaa atgaaacaga caaaaaaagc ggatcaaaac
    13021 tgaagacagt ttcgatccgc ttttttctat caaagttagt gactacttca tcgtatactc
    13081 tgataagaat gatttctagt aaaatatact tggtttgaaa aactttgtca acgtgattta
    13141 ttcaaaaagt aatggaatca ataatatttt ttagcaggtt ctactactaa ataacgataa
    13201 ggttcctcac cttccgaatg tgtttttaca tgttcatctt tgcttaatgc agcatggatt
    13261 tgtttacgtt caaatgcagg cattggttct aagaaaacag gctgccctgt ccgttttact
    13321 ttttcagccg tgcgatcagc taaacgctga atgatctctt ggcgcttttc acggtaattg
    13381 cctacattga ctactacaga cagcttattt cttgctaaac gatggatgaa gacttgtgcc
    13441 agatattgca atgcattcag tgtttttcca tgtttcccaa tcagtatgcc ttgcttttgt
    13501 gtttctaaat ggaaaacaac catattattt gattctcttt ccattttgac aagagctgga
    13561 gcatcaagct gttttgaaat ttcagtaaga taaactgcca attccgttaa agctgcttca
    13621 tcacctagct cacttacttc ttctgttttt tcttcaagaa tcgtttcgtt gactacctcg
    13681 atctcagctg ttggttcttc ttccaaaatt tcttctacag tctgttctac tgtttcagaa
    13741 actttttcgc tgatacttgg ctggatcgaa agccgtgcat tctttcgacc cattcctagg
    13801 aaaccttttt ttccttcttc cagtatttcg atttcgactt ggtctttggc caaacccaaa
    13861 tcagccagac ctagttggac agcttcgtct attgttgcag cttcataaat cggcattagc
    13921 attacctccc ctattatttc cgttttttct taggattcat tgctttctta agagcacgtt
    13981 ctctttcgcg gattctccgt gcttcttctt cacgctcttt tcgaattttg aaagggttgt
    14041 tgatcagcag cgtttgtaca acttggaagg cattggaaac tacccagtat aatgaaagac
    14101 cgctggctaa attgacaccc atcactaaaa tcattacagg catcacgaaa ttcatgatct
    14161 tcattgatgc attcgattca atttgactca tgctagataa atacgtactt gcaaaagtaa
    14221 agattgctgc taaaatcggc aaaataaatg ttggatccgc gttccctaag ttcaaccata
    14281 agaaatgccc ttgtttcaac tctggtacac gtgaaataga ttgccataac gccatcaaga
    14341 ttggcatttg tactaataaa ggaagacaac cagcatatgg attgacgcca ttttctgcat
    14401 ataaacgctg ctgctcttct cttaacttac tttgtgtttc cggatctctt gaactatatt
    14461 gttgctgcag tgcttttaat ttcggttgta attcttgcgt tttacgcatg ctctttgttt
    14521 ggaaatgcat caacggcaat aggatcacgc ggatgatcag tgtaaataaa atgatcccta
    14581 tccctgcatt accaaatgac aacgccttga tcgcctcagc aaagtaataa acaatataac
    14641 gatcccaaat tcctgtactc tgtgcgctga cttccccagt tccgcaaccg gataagacga
    14701 tgaccaaact aacgataccg gccatcaaaa gtaaacgttt gtatttcttc acttactctt
    14761 ttcctctctg tcgattattt ttgcaagttt taagacgtgg actaaattgg agcgaacctc
    14821 atcataagtc aaccccttca cacttggtct agcaatcaca ataaaatcca actccggatc
    14881 aatttcttct tttatagaat aaactgctgc tcggattttt cgtttgactt cattgcggtt
    14941 taccgcgttg ccgacctttt ttcctacaga taaaccaact cgaaaatgag gctgtcccgg
    15001 tttttccaaa cggtaaacga cgaatttgcg gttagcaaaa gaatctcccg tatggaacac
    15061 ggcttgaaac tctcgttctt tttttactcg atacgctttt ctcattagtt ttctttcctt
    15121 ttctatctct cacttacgct cataatcata ccataattac gattgtcctt tcatcatttc
    15181 cctaaaaaag agtataaaaa aaaccactga aaccccagtg gcctaagctg aaagaacttt
    15241 tcttcctttg cgacgacggc tagctaacac acgacgtcca tttttagtac tcatacgttt
    15301 acggaatccg tgaacttttt gacgtttacg tttatttggt tggtatgttc ttttcatata
    15361 ttccacctcc aaaatgttct tatctatttt ataaacacag acatactttg atagtataac
    15421 gacaaagtag tgattttgtc aatataaaat agaagatcga ttgttaaaac ttatccacaa
    15481 gattattttt ttctatggga atcctatgtg gaaaaagtta tgcacaggga ttatccacag
    15541 ataataaaag gctggtttta cacagaaaac acacgtgtaa aaaaagtaat ccacaatcag
    15601 gcgatttcct gtgtattccg tctcctaact attggtatat ctgtattttt tatccacaga
    15661 cgcaagagga aatctattgg tttttacgag ttttccacct gtgcattagt ggtgtggaaa
    15721 actttttttg acaactgtgt ttttctcttt tttctttttg tcgattgtgt ggataattgt
    15781 aattaaaaaa tttttttctt cggtttctgt ggaaaactat tgaaaaaaat gctaaattag
    15841 tactagttat tcacttttta atgaaggagg aggccttata tggtatccct cgatgcttta
    15901 tggaatgaat taaaagcaac ataccaaaag gatttatcgc ctgctagcta taatacatgg
    15961 atcgaaacag cgaaccctcg aacccttgat caaaaccagc tcgttgttga ggttcctagc
    16021 aagattcata aagaatattg ggaaaaaaac ctggcgacaa aaatcgttga agtcggttat
    16081 atgttatcag gaaatgagat tatccctcgc tttattactg gcgaagaagc agagcaagaa
    16141 gaagttatag aagaaaaaaa tccaaaagtt gtggcaccta gtccgctaaa aaaagccatg
    16201 ctgaacccta aatatacctt tgatacgttt gtcattggta aaggaaacca gatggctcat
    16261 gcagcagcgc tcgttgtagc agaagatcca ggttctattt ataacccgct cttcttttat
    16321 ggaggcgtgg gacttggtaa gactcacttg atgcatgcaa ttggtcatca aatgctgcaa
    16381 agtcagccta atgccaaagt aaaatatgta agcagtgaaa cttttgctaa tgatttcatc
    16441 aattcgatcc aaaacaaaac agcagaagag ttccgtcaag aatatcgaaa tgttgattta
    16501 ctgctagtgg atgacattca attttttgca gaaaaagaag ctacgcaaga agagtttttc
    16561 catactttca atgccttgta caatgaaggg aaacaaatcg tactaaccag tgatcggttg
    16621 ccaaacgaga ttccgaaact gcaagaacgc cttgtttctc gctttgcatg ggggctgtct
    16681 gttgatatta caccgccgga tcttgaaaca agaacagcga ttttacgtaa aaaagcggcg
    16741 gctgaacgtc tagagatccc agatgacaca ctaagctaca tcgcaggaca aattgattct
    16801 aacattcgtg aattagaagg tgcgcttgtt cgagtgcaag cttttgctgc gatgaatagt
    16861 gaagatattt cgactagtct agctgcagac gcactgaaaa cgttaaaatc aggaaaagga
    16921 catcctcaat tgtcaatcct gcagatccaa gaagaagtgg caaagtatta tcacatccaa
    16981 ttgaaagatc tcaaaggaaa gaaacgcgtc aaatctattg ttgttcctag acaaatcgct
    17041 atgtatttgg cacgtgagct cacagataat tctttaccga aaatcggagc tgaattcggt
    17101 ggcaaggatc atacaacggt tatccatgct catgaaaaaa tccagcagct gatggattct
    17161 agtgtcagta tgcaaaatga agtatcagaa atcaaaaact ttttactaaa ttaatttgtc
    17221 gattctatca actgaaaata gcatgtggat aaaagaaaaa aacgttcaaa agttatacac
    17281 actttttcca cagctgtttt ttcgtttctc tcttacgttt tttgagtttt ccacagaatc
    17341 aacatgccct attactttta ttattctttt taactaataa taaataaaca aacattcaaa
    17401 ggagatacat catgaaagtt actttaaacc gagctagctt tatgcaggaa ttgcaaactg
    17461 ttcaacgagc tatttcaagc aaaaccacga tccctatttt gacaggtgta aaaatcacac
    17521 tgacacaaga aggtttgact ttgacgggga gcaacgctga tatatcaatt gaaacttttt
    17581 tgtctgttga aaacgaaaaa gcaaatatgc aaatcgaatc tactggttcc attgttttac
    17641 aagcacgttt ctttagcgaa atcattcgga gacttcctga agaaacattt actttagaag
    17701 ttttagaaaa taaacaagta gcgatcactt ctggaaaagc gaattttatc gtaaatggat
    17761 tagatgcaga taactatcct catcttcctg ttgtcgaaag ccataaccag atgaaattac
    17821 ctgtacacgt attgactaaa ctaatcaacg aaacagtttt tgctgtctca caacatgaga
    17881 gtcgtccaat cttgacaggt gtgcatttca ttttatctga taattcttta ttagctgtag
    17941 ctactgattc tcaccgtcta agtcaacgcg tgattccagt agaacaagcg gctgatcatt
    18001 ttgatattgt tattcctgga aaaagtttga tcgaattatc tcgctcatta acaaatgaag
    18061 aagaaatcgt cgaaatcagc attatggaaa accaagtgtt gttcaaaaca gaaacgatgt
    18121 atttctattc ccgtttgcta gaaggaaact atccagatac caatcgtttg attccttcaa
    18181 gttttaatac agaggttgaa ttttctgttc ctagcttttt agcggcgatc gaacgggctt
    18241 ctttactttc tcatgaaggc cgtaacaaca tcgttcgttt atctattcgt ccagatgcgg
    18301 ttgttttata cggaaattcg ccagaaatcg ggaaagtaga agaaagcttg agttatacag
    18361 caagcagtgg cgatccatta gatatttctt tcaatccgga ttatatgaaa gcagcgttgc
    18421 gtgcatttgg agatatgagc atcaaagtga aatttatctc tgctattcgt ccatttacat
    18481 tggaaccaac ggaagatggc gttcaattta ttcagctgat tactcctgta cgtacaaatt
    18541 aacaatgatt ttcaatgttc ttgaaaattt aattgaaaag aaatagaaaa aataaaattt
    18601 taaacagaag gccctaaaat ctcttttaag gctttctgtt tatttttgtt taaatcacct
    18661 tttttagatt aaatcgtctt atatggaaaa atatatggat aaatttgttt tattgcgaaa
    18721 aaaaaggtat aatagagtat acttttaata atgaaaatga gggatgggca ttgaaacaac
    18781 aagtcattct aaatacggaa ttcatgacgt tgggccaaat gttgaaagaa gtcacagtta
    18841 tcggtagtgg cggacaagca aaatggtatt tggcagaaaa cactgtgtta gtcgatggcg
    18901 aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat gatggttgaa gttccagaag
    18961 taggaacttt ttttatggta aaaaaaggaa acgacgataa tgaggctgaa taagctgtat
    19021 ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat tttccaagaa cctcgtcatc
    19081 tttttaggag aaaatgcgca aggaaaaacg aatcttttgg aaagtatcta cgtattagct
    19141 atgacgcgaa gtcatcggac aagtagtgag caggaattga ttggctggac tgacgatcag
    19201 gcgatgattc aaggagagat cacaaaaggt tcatccactc ttccattaga aatcttatta
    19261 tctaaaaaag gccgaaaaac caagatcaac catatcgaac agaaaaagtt aagtacttac
    19321 gtgggacaaa tgaatgtcat tttatttgca ccagaagatc tttctttagt aaaaggcagc
    19381 ccgcagacca ggcggaagtt tttagatatg gaattaggcc agatcaatcc aatctatctt
    19441 tatgatcttg tccaatacca agcaattttg aaacaaagaa accagtactt gaagcaattg
    19501 aatgaaaaaa aacaaactga tttgctttat ttggatattt tgtccgaaca actagcagcg
    19561 tttggcagca aagtattgaa agcaagagca cagttcgtca agcgattgga gttttgggct
    19621 aattctttgc atcagcaaat cacccatcaa aaagagcagc tggaaatcga gtatctcacc
    19681 gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc aagaacaatt tctcgcactt
    19741 ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa cgacagttgg tcctcatcgt
    19801 gacgatcttt ccttttttat taatcagaaa aatgtgcaga cttatggatc gcaaggacag
    19861 caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg atttgattaa agaagagaca
    19921 ggggaatatc caatcctttt gttagatgat gtgatgagcg agctggatga taatcgtcaa
    19981 ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt tcttaacaac aacgacactt
    20041 gaccatgtaa aaaataaaat gactgttgaa cctgagattt tttatgttca acaagggaaa
    20101 ctaaaaggag aagaaactga atgacagaag aaagaagttt agtagaacgc gctaaagagt
    20161 atgatgccag tcagattcaa gtacttgaag gacttgaagc cgttcgtaaa cgacctggca
    20221 tgtatatcgg ttcgactagt tctgagggtc tgcatcacct tgtttgggaa attgtcgata
    20281 attcgatcga tgaagtatta gcaggttttg caacgaaaat ccatgtgatt attgaaaaag
    20341 acaacagtat caccgttatt gacgacggtc gggggatacc tgtagatatc caggcaaaaa
    20401 ctggacgtcc tgcagttgaa acggtcttta ctgttttaca tgctggagga aaattcggcg
    20461 gtggtgggta caaagtttct ggtggacttc acggagtagg atcttctgtt gttaacgcgc
    20521 tttctacaca gcttgatgtc aaagtataca aagaaggaaa tgtgtactat caagaatatc
    20581 gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca aacggatcgc catggaacaa
    20641 cggttcactt tacgccagac cctgaaattt ttacagagac aactgagttt gatttttcaa
    20701 aattggctac ccggatccgt gaacttgctt tcttgaaccg cgggatgcgt atatcgattg
    20761 aagacaaacg agaagaagaa ccagtcatca atgaatacca ttatgacggc gggattaaaa
    20821 gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt tccagaacct gtatatttgg
    20881 aaggtgaaca gcaagatatc gcagttgaag tctctatgca atatacagac ggctaccatt
    20941 ccaatatcat gagttttgcc aacaatatcc atacgtatga aggcggaaca catgaatcag
    21001 gattcaagac tgctttaact cgtgtgataa atgattatgc ccgaaaacaa aagctgatga
    21061 aagaaaatga cgacaacttg accggtgaag acgtgcgaga aggattgaca gctgttattt
    21121 ctatcaagca tcctgaccct caattcgaag gacaaacaaa aacaaaattg ggcaattccg
    21181 aagtacggac agtcactgat cggttattta gcgaacattt catgaaattc ttgctggaaa
    21241 acccaagtgt gggccgtcaa attgttgaaa aaggattact agcttccaga gctcgtctag
    21301 cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc tttagagatt agtaatctgc
    21361 caggaaaatt agcggactgc tctagcaacg atcctgaaaa atgcgaattg ttcatcgtcg
    21421 aaggagattc ggccggcgga tcagctaagc aaggtcgtaa tcgagagttt caagccatct
    21481 tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag catggacaag atcttggcaa
    21541 atgaagagat ccggtcttta tttactgcta tgggtacagg gtttggagaa gattttgatg
    21601 tatcgaaagc tcgctaccac aaattagtta tcatgactga tgccgatgtc gacggcgcgc
    21661 atatccgtac attgctgctg actctttttt atcgttacat gcgtcctatt gttgaagctg
    21721 gatacgtcta tattgctcag ccacctttat acggggtaaa acaagggaag aatattacgt
    21781 atgtacaacc aggaaaaaat gcagaagaag agttgaaaca agtcgttgct tcacttcctg
    21841 cctcaccaaa accaagcgtt cagcgttata aaggacttgg agaaatggat gatcatcaac
    21901 tatgggaaac aactatggac ccaagcaatc ggatgatggc ccgcgtcagt gtggacgatg
    21961 ctattgcagc agatcagata tttgaaatgt tgatggggga tcgagtagaa ccccgtcgtg
    22021 cattcatcga ggaaaatgca cactatgtga agaacttaga tatttaatca tgaaggagga
    22081 aaaagaatga gtgaagaaat cagagaaaac atccatgatg tcaatctaac cagcgaaatg
    22141 aaagactcct tcatcgatta tgcgatgagt gttattgtcg cacgtgcact tcctgatgtc
    22201 agagatggtc tgaaacccgt tcatcgccgt attttatacg gcatgaacga attaggtgta
    22261 acaccggata aagcccataa aaaatctgcc agaatcgtcg gagatgtcat gggtaaatac
    22321 catcctcatg gagactatgc gatctatgag tcaatggtac ggatggcaca gccattcagc
    22381 taccgttata tgctagtgga tggtcatgga aactttggtt ctgttgacgg tgacggtgcc
    22441 gccgctatgc gttataccga agcacggatg agcaaaatcg ctacagaaat gctgcgtgat
    22501 atcaataaga acactgttga tttccaaagc aactatgatg atacagaaaa agaaccagtt
    22561 gtactacctg ctcgcttccc gaatctttta gtgaacggaa caacggggat cgccgtaggt
    22621 atggccacga atatcccacc tcataacttg tctgaggtag tagcggctat cgatttgctg
    22681 atggataatc ctgatgtgac gaccaatgaa ttgatggaag tattacctgg accagatttt
    22741 ccaacaggtg gtttggttat gggaaaatca ggaatccgaa gagcgtatga aacaggtaag
    22801 ggttctatta ccgtacgtgc aaaagttgag attactgaga tgccaaatgg taaagaacgg
    22861 atcatcgtga cagaattgcc ttatatggtc aataaagcaa aattgatcga acggatttct
    22921 gaactgcaca gagacaagcg tatcgaaggg atcacagatt tgcgggacga atcttcccgt
    22981 gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca gcgcatctgt tatcttgaat
    23041 aacttatata aattgacatc cttgcaaaat tcatttggat tcaatatgtt ggcgatcgaa
    23101 aaaggtgtgc cgaaagtttt gagcttgaaa caaattcttg aaaactatat cgaacatcag
    23161 cgcgaagtca tcacaagacg gacagtcttt gaaaaagaaa aagcagaagc aagggcacac
    23221 attttagaag gtttgcgtat tgctttggat catatcgatg aaatcattgc gattatccgc
    23281 gggtctaaat cagatgacga agcaaaagcg acgatgatcg aacggttcga tttatccgat
    23341 cgccagtctc aagctatttt agatatgcgt ttacgccgat tgaccggttt ggaacgggaa
    23401 aaaatcgaaa atgaatacca agaactctta aaactgattg aagatttaac tgacattctg
    23461 gcaagaccag aacgtgttac agaaatcatc aagaatgaat tagcagactt gaatcaacga
    23521 tttggtgata aacgacgcac agaattacta gtcggtgagg tcttaagtct ggaagatgaa
    23581 gatttaatcg aagaagaaga aatcgttatt acattgacaa acaatggtta catcaaacgt
    23641 ttggcaaata atgaattcag agctcaacga aggggtggac gtggtgtcca aggaatgggt
    23701 atccacaatg acgacttcgt caaaaatctg gtttcttgtt ctacccatga tacattgtta
    23761 ttctttacga ataacggaaa agtgtatcgg gcaaaaggat acgaaattcc agaatacggg
    23821 cgcacagcca aaggaatacc aattatcaat ttactaggta tcgattcgag tgaaagcatc
    23881 caggcaatca ttgcggtgac gggagaagca gaagaaggcc actatctctt ctttacgacg
    23941 cgtcaaggta cagtaaaacg tacttctgtc actgcttttg ctaacatccg aagtaatggt
    24001 ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca atgtgctact gacagatggt
    24061 caatctaata tcattattgg aacacataac ggctactctg tcactttcaa agaagaagca
    24121 gtccgcgata tgggacgtac agcttccggg gttagaggaa tacgcctgcg tgaggaagat
    24181 tatgtcgttg gggcagcctt aatgaaggaa gatcaagaag tattagtgat cacagaaaaa
    24241 ggttacggaa aacggacaaa agtatcggaa tatccagtga aaggccgcgg aggtaaaggg
    24301 atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag ctggattgac cactgtttct
    24361 ggagatgaag acatcatgct gattacagat aaaggtgtca ttatccgttt caacgtatcg
    24421 actgtttctc aaactggacg ttcaacatta ggcgtacgtt tgatgaagat ggaagctgat
    24481 acgaaagttg tcacgatggc agcagtcgaa ccagaagctg cagaacaaac agttgaagaa
    24541 caaggatcag aagaatagcc atcaagacta attgtgtaag ctagcaagaa tgaccgatta
    24601 aaaaatctgg ttattcctgc tggctttttt gattagttga atttctctta aaatattcga
    24661 aactgttcat tctgtattgc ttttgaccaa aatagagtgt ataatcagag cttgtgagta
    24721 gtctaattaa ttagataact ctccttgctc ttaggaacct aagagccgaa agtccacagg
    24781 gaggtgaata tcaatggaaa acacgaaata tgaaatcatg tacattattc gtccaaacat
    24841 tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat gcaatcttga aagataacgg
    24901 agcagaagtt atcgaatcaa aagattggga aaaacgccgt ttcgcttatg aaatgaacgg
    24961 ttaccgtgaa ggtatctatc acatcgttaa agtatcttct ccatcatcag caaatgctgt
    25021 caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc attcgtcaca tgattgttaa
    25081 agaagaagct taattgttgt ttcatgtgaa acaatagaaa acgatgagag gagatgacta
    25141 ttttgattaa taatgttgta ttagttggac gtctgacgaa agatccagat ttgcgttaca
    25201 cagcaagtgg tacggcagtc gcaacattta ctttggctgt gaatcgtaat tttacaaacc
    25261 aaagcggcaa tcgcgaggct gattttatca actgtgtgat ttggcgcaaa tcagcagaaa
    25321 ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt tactggacgt atccaaactc
    25381 gttcttatga taatcagcaa ggacaacgtg tatacgtgac agaagtagta gctgacaact
    25441 tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca acaaagcggc ggattccaaa
    25501 gttctggtca atcagctggc ggatttggcg gaaacaataa ctctaatcaa acatctcaat
    25561 catccaacgg tatgcctgat ttcgaccgtg atacttctga cccattcggc tcaagttcaa
    25621 cgatcgacat ttcagacgat gatttaccat tctaaacgaa tttgatagga gggaaataga
    25681 atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag tagactacat tgctgctaac
    25741 catattgaat atatcgatta taaagatatc gaattactaa aaagatttat ttcagaacgc
    25801 ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta aaaaccaacg taaaattact
    25861 gttgcaatca aacgcgctcg tatcatgggc ttgctacctt tcgttagcga agactaataa
    25921 atcctaggaa ttgaatgact gatccttttg atcagctatt caattccttt ttttctgtaa
    25981 aaataacctc tattcgttta gtcgctcgaa tgacgaaagt tatggtaaa
//
LOCUS       DACCCM010000038        26027 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCCM010000038 DACCCM010000000
VERSION     DACCCM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.039"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IVK78_001530"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IVK78_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP6375008.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IVK78_001531"
     CDS             808..1242
                     /locus_tag="IVK78_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP6375009.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IVK78_001532"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IVK78_001532"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP6375010.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IVK78_001533"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IVK78_001533"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP6375011.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IVK78_001534"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IVK78_001534"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP6375012.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IVK78_001535"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IVK78_001535"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP6375013.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IVK78_001536"
     CDS             9513..10439
                     /locus_tag="IVK78_001536"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP6375014.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IVK78_001537"
     CDS             10621..11514
                     /locus_tag="IVK78_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP6375015.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IVK78_001538"
     CDS             11648..11773
                     /locus_tag="IVK78_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6375016.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IVK78_001539"
     CDS             complement(11907..12539)
                     /locus_tag="IVK78_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375017.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IVK78_001540"
     CDS             complement(12687..13229)
                     /locus_tag="IVK78_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP6375018.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IVK78_001541"
     CDS             13364..13753
                     /locus_tag="IVK78_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375019.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IVK78_001542"
     CDS             13757..14521
                     /locus_tag="IVK78_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6375020.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQAGEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IVK78_001543"
     CDS             14650..15597
                     /locus_tag="IVK78_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6375021.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IVK78_001544"
     CDS             15727..16467
                     /locus_tag="IVK78_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375022.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IVK78_001545"
     CDS             complement(16758..17426)
                     /locus_tag="IVK78_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6375023.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IVK78_001546"
     CDS             17560..17847
                     /locus_tag="IVK78_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375024.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IVK78_001547"
     CDS             18263..19801
                     /locus_tag="IVK78_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP6375025.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IVK78_001548"
     CDS             19798..20763
                     /locus_tag="IVK78_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375026.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IVK78_001549"
     CDS             20750..22072
                     /locus_tag="IVK78_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6375027.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IVK78_001550"
     CDS             22313..23056
                     /locus_tag="IVK78_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6375028.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IVK78_001551"
     CDS             23071..24063
                     /locus_tag="IVK78_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6375029.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IVK78_001552"
     CDS             24075..24812
                     /locus_tag="IVK78_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375030.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IVK78_001553"
     CDS             24802..25524
                     /locus_tag="IVK78_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6375031.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>26027)
                     /locus_tag="IVK78_001554"
     CDS             complement(25608..>26027)
                     /locus_tag="IVK78_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6375032.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8953 a         4426 c         5429 g         7219 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctggcgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DACCCM010000039        25971 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCCM010000039 DACCCM010000000
VERSION     DACCCM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.040"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1273..1938)
                     /locus_tag="IVK78_001555"
     CDS             complement(1273..1938)
                     /locus_tag="IVK78_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6375033.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IVK78_001556"
     CDS             2064..4100
                     /locus_tag="IVK78_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP6375034.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IVK78_001557"
     CDS             4104..4742
                     /locus_tag="IVK78_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375035.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IVK78_001558"
     CDS             4770..5198
                     /locus_tag="IVK78_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375036.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     EKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IVK78_001559"
     CDS             5655..6566
                     /locus_tag="IVK78_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6375037.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IVK78_001560"
     CDS             6783..7220
                     /locus_tag="IVK78_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375038.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IVK78_001561"
     CDS             7315..8199
                     /locus_tag="IVK78_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6375039.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IVK78_001562"
     CDS             8423..11590
                     /locus_tag="IVK78_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375040.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IVK78_001563"
     CDS             11610..12155
                     /locus_tag="IVK78_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP6375041.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IVK78_001564"
     CDS             12183..12638
                     /locus_tag="IVK78_001564"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP6375042.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IVK78_001565"
     CDS             12635..13393
                     /locus_tag="IVK78_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6375043.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="IVK78_001566"
     CDS             complement(13479..14219)
                     /locus_tag="IVK78_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6375044.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="IVK78_001567"
     CDS             14280..15533
                     /locus_tag="IVK78_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6375045.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="IVK78_001568"
     CDS             15855..16082
                     /locus_tag="IVK78_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6375046.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="IVK78_001569"
     CDS             16205..16531
                     /locus_tag="IVK78_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6375047.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="IVK78_001570"
     CDS             16599..17312
                     /locus_tag="IVK78_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6375048.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17622..18473
                     /locus_tag="IVK78_001571"
     CDS             17622..18473
                     /locus_tag="IVK78_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6375049.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18594..20459
                     /locus_tag="IVK78_001572"
     CDS             18594..20459
                     /locus_tag="IVK78_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6375050.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20507..21916
                     /locus_tag="IVK78_001573"
     CDS             20507..21916
                     /locus_tag="IVK78_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP6375051.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22024..22725
                     /locus_tag="IVK78_001574"
     CDS             22024..22725
                     /locus_tag="IVK78_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6375052.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22884..23363
                     /locus_tag="IVK78_001575"
                     /pseudo
     CDS             22884..23363
                     /locus_tag="IVK78_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24211..24786
                     /locus_tag="IVK78_001576"
     CDS             24211..24786
                     /locus_tag="IVK78_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP6375053.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24786..25226
                     /locus_tag="IVK78_001577"
     CDS             24786..25226
                     /locus_tag="IVK78_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6375054.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25242..25943
                     /locus_tag="IVK78_001578"
     CDS             25242..25943
                     /locus_tag="IVK78_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6375055.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         8767 a         4221 c         5360 g         7623 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagagaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggcagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tattttttat atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata
    17521 agaaatggat tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg
    17581 aattctaggt ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
    17641 tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa
    17701 aaggactagc ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag
    17761 aatttgtttt aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc
    17821 cggcagagga gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag
    17881 ttgaattttc gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac
    17941 gagcaaaaga aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aaattttatc
    18001 cgaaagaata tcaagcagcg aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg
    18061 aactttctga ggatgaagcc ggttttattg cctttcattt tgtgaatagc caacaaggaa
    18121 acgaagacat gcaagtgacg atgaccgcta ccacaatggt aagagatata ctgagtatca
    18181 tcagtaaatt cttcggcatc gtgttcgatg aagaatcttt gaattatcag cgtatcatta
    18241 ctcatctgca gttttttgcc cagcgttatc tcaaagggga aggatctgac gagcaagatg
    18301 aatttctcta cgcattagtt caaggaaagt atccaaaagc gtttcattgt gtcgaacgga
    18361 tcaatgagta tcttcgacaa acgcaaaaac aggaaatggg gatcgcagag cagatttatt
    18421 taacgattca tattcaaaga gtagtaagtg agaaaaaaag catccaatca taaggattgt
    18481 tactgtatgg caggcaaaac ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt
    18541 tttttcaatc tgggttttta ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata
    18601 atcaagcagt tggtcgtcgt gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt
    18661 tagtacattg tgctacgcgg ttgcgattca gattaaaaga tgaatcttta gcagatacgc
    18721 aaaagttaaa agaagacccg gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag
    18781 tagtgattgg cagtaatgta gcagatgttt atcaatcgat cgtggatgag gaaggtcttg
    18841 cagtttcgga ggatggaaaa gaagagtcca aaaatatttt gaatcgtctg atcgatatta
    18901 tctctagtat atttacacct tttctagggg cgatggcagc cgccggggta ttgaaaggat
    18961 tcttatcatt ggcaacggtg atgggctggc tgacagctga tagcggtact tatcaaatat
    19021 tgtttgctgc agcagatggt gtctttacct tcttacctgt catgcttgct tttacagcag
    19081 ctaaaaagtt caaagccaac cagtttttag cagtagcaat tgctatggca cttgtgtatc
    19141 cagctatcac agcagttgct ggtgcgggag aagctatcag tttctttggg atcccagtga
    19201 ttctttcacc atctggttat acttcttctg tcataccaat cattctagcc gtatgggttc
    19261 aaagtaaatt agagccattc gtaaaaaaag tgattccgca atttctgcaa atgattttag
    19321 ttccgcttgt cgttttagta gtgatggttc cattaacatt tcttgcttta ggaccaatcg
    19381 ggacggttgc cggtaatgct ttaggcggtc tcttcaactc gatttatgga tttagtccaa
    19441 tcgtagcagg tctaatcatg ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt
    19501 ggggattcgt accgatcatg tttttgaata tcgaacaata tgggtttgat gtattgatgc
    19561 caatgcttct tccagccatc ttggcacaag gtggggctgc tttagctgta gcattgcgaa
    19621 caaaagatac gaaacttcga gcattaggga tttcttctac agtgacttcg ttatttggaa
    19681 tcactgaacc aactgtgtat ggtgtgacat tacctttgaa gaaaccattc attgccgctt
    19741 gtatttctgg aggtattggt ggagctatta tcggattttc cggagtaaaa gctttctcca
    19801 gcagtttggt cagcttattg acgattccga ctttcattaa tacggtggat ggtgtagagt
    19861 caaatgtgac agtagcagta atagctacag gaattgcttt tgttcttgca ttcgtaggta
    19921 cattgatttt aggatttgac gaacaaacac aagacaatca attagaaaac aaacatgcga
    19981 atgcaggaga accgattact tcagcacggc ataccttaaa aagtccttta acgggtaaag
    20041 tactgccatt gagtgaagta ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta
    20101 tcgctattga tccagaagta ggagaactag ttgcacctgc agacggagag atcacgacta
    20161 tttttccaac tggacatgct gtagggatca caacgactga cggagcggag attttgattc
    20221 atatcgggat ggatacagta gaattaaatg ggaatggctt tgagatctta gtcaaacaag
    20281 gggatctcgt caaagcagga gatttattga ttcgttttga catcgaagca attagagcag
    20341 ctggctatag cgtaatcact ccagttgtca ttacgaatac tgatgcattt gccgatatac
    20401 ttgaattaga ccaaaaagag attattgcaa acgaagatgt tctggcaatt gtaaaataag
    20461 gaggaaatca ggatgcaatc acgtttttca gaaaaatttt tatggggtgg agcaactgct
    20521 gccaaatcaa ttagaagggg cttatgatcg agacggaaaa ggactatccg ttgcagatgc
    20581 tatgcctgga ggtaagcagc gctttgcgat tatcggcagt gaagaatttg actggacgat
    20641 tgatcaagaa aaatatattt acccgaatca tcgaggaatc gatcattacg atcgtttcaa
    20701 agaagatctt gccttgtttg cggaaatggg attcaaatgt tatcgttttt cgattgcctg
    20761 gacacgaatt tttccaaatg gagacgaagg tactcctaac gaagcgggat tagaatttta
    20821 tgatcagctg attgatgagt gcttaaaata tgatattgaa cctgtgatca cgatttctca
    20881 ttacgaaatg cctcttcatt tggcaaaaga atacggtggc tggaaaaatc gaaaactgat
    20941 cgacttctat gaacgatttg ctcagacagt attagagaga tacagcagta aagtcaaata
    21001 ttggatgact ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa gtcaaggatt
    21061 agtaaaaagc aatggagcag gagagtacca aaatattttc caagcatggc ataatcaatt
    21121 cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg cgttcggata tccaaatcgg
    21181 ctgcatgatt atctatgcaa caacttacag catcgatgcc aatccagtca atcaggcagc
    21241 aacaatgatc caaaaccaag aattcaattt tttctgcacg gatgtccaag tacgtggaga
    21301 ataccctgca tacacagcgc gtacccataa aaaatatgga gtcgatccgg aaaaattaga
    21361 gcaaacggaa aaagacttcc agttgctaaa agaatatcca gtggactaca tcggatttag
    21421 ttattatatg tctacggcaa tcaatgagac tgatccacaa gcagcaactt cagaagggaa
    21481 cttgctggga ggagtaaaaa atccgtttct tgaggcaagt gaatggggct ggcagatcga
    21541 tccagaaggg ctgagaatcg cattgaatga attatacaat cgttaccaga aaccattatt
    21601 cattgttgaa aacggtcttg gagccattga tcatatggaa gaggatggca cgattattga
    21661 tgattatcgg atcgattatt tacgtcgtca tatcgaagcg atggctaatg cagtagcaga
    21721 cggtgtagat ttgatgggct atacgccttg gggatgtatc gatcttgtca gtgcatcaac
    21781 aggagaaatg agtaaacgtt acggctttat ctatgtggat ctagacgatg aaggaaatgg
    21841 gacactgaat cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa
    21901 tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc acatgtgatt
    21961 tgtgttcttt ttttattttt tatgtgctat tcttttagta atagtaaagc aaagggtgac
    22021 gttatgaaaa ggacacgtgt actatacttg gaagtcgcag ataaaattaa agaagatatt
    22081 ttctcaggaa aatatccagt cggttcgatg ttgccgacag aaagtgaatt agaagaactg
    22141 tttcaagtca gcaaaatcac tgtccgaaag gcaatcgaac tgctggcatc agatgaatat
    22201 gtcgcaaaga aaagtggtcg tgggacgacg gttctaagca atcgtcctta caatcgatta
    22261 tccaaagcgg caagctttac gcaaatcttg gagaactccc gatatgaagt ccggaaagaa
    22321 acgctggatt tgcaattggt ccatataaag aaggaagatc ctttgttttc ctttttcggt
    22381 gagcaagctg tttgttttaa gcgcttatat tatttgaaca atcagcctta tatttatttt
    22441 gttcattatc ttccacaaga aatgatgcaa ttcagcagag aagaatttgg caaagaatct
    22501 ttgtatcgtt tgctgacgaa aaaaggatat actattgact cattttctga tgatttctcg
    22561 gcagtatttt taacagaaga agaacaagga attctgaaaa cagacgcaca gatcggcatc
    22621 aaacggatac ggaaatcgtc tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc
    22681 taccatacgg agctgcatcc ttaccatatt gattatgaaa cataaataaa aaaagaggtt
    22741 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    22801 ttttcgtttg gttaggctta ttaccgcaga tacacggctg tttctggatt accatttatt
    22861 ctggataagg gggtgtggca cgattttcag aaggaatcta tgaaccaatc actgtattga
    22921 ttttagcttc gatggtaacc ccattcggct tgacgatcgc ttatttctta gggaaaatca
    22981 ttcgtaaaaa tatactcaat cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg
    23041 ggatctgtca gatcacagag ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg
    23101 tcatagcaac gggagtcggt gggacagtag gtggcggatt aagcatgttt tggggcgcag
    23161 atagccgtat tccggcatcg ggaatgtttg cagtcgctac gatgaccaga ccatgggcat
    23221 tcattggtgc attgttagct ggttcttttg tcacaggaat aaccatttta ttattgaaaa
    23281 aaccagtaga tccgaatgca gaaatcatcc aagaagaaaa agaggaagaa gacatatctt
    23341 gggatgattt gacaattagt taggagaggc agaatggatt acaagaaaaa agtacaatag
    23401 atgtaccttg agacaggaat ggtatgaaag aatcagagtt agaaagaatc gaatatacag
    23461 attttggttt aggagcgatc gaaactgtcc agagcatcgg caccctcctt gaaatgacga
    23521 tgaaggaaaa atgagaacct ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga
    23581 aaggaaacaa aaaaactttc ctgtactatt ccaaaaagga aagaaagtta ttatacagta
    23641 aaaaaagaaa ttattcttca tgctagagat cagtacacgt tcgagcccaa tatcaaacac
    23701 tagttccagg caggcgcaac aggtgcggtt atcactgaat tttcttccag cagtgacgat
    23761 tcaagtgata tttttacaaa tccagctatt tatcgttaac aaaaagattt ttaggagaag
    23821 ctgatgaaca aaaatgatca aattttgatc gtttagttcg tcggcttttt tatttagaga
    23881 aacatcattt atcagtgaaa cataacacga gaacaaaatg attttttcgt taataaaaga
    23941 aacaagtatt tgttgtttaa aataatacac gttattggca tataggacga aatcccagta
    24001 tcttctataa gtaagtcatt tcatatcgat gaaaacacgt tatttatttt tgttttatct
    24061 ataaaatggc tgaaaaatga tttttttcta aatactatat atagtggcgt ctatcctata
    24121 taccactaaa aaactacaaa atctagtctt ttattttaaa aaaacacgtg tagaatgaag
    24181 atagactttt gagttaggga gtgttttttt atgaaactta ctgttttttg cggttcccgt
    24241 tttggtaata aagaaagtta caaatttatt gcgcaaacgt taggaaaata catggcacaa
    24301 gaagaaatcg aactggttta cggtggttca tactccggga ttatgggagt gattagtaaa
    24361 acagtactgg aaaataacgg aaaagtcaca ggtatctatc cgaatgggct ttttgaaact
    24421 gaattgcctg gaaaagatgt tactacattt attcctacag aaacgatcga tgaaagaaag
    24481 gtattattat ttgaaaaggg agatgctgtc ctcgttttcc ctggaggtct aggtacattg
    24541 gaggaattct cacaacttct ttcttggata gcaatcggtc tgacgcccga caagccaatc
    24601 ggaatattga atattggcgg ttattacagc ggcttgcaac aattattaga aacttttgca
    24661 aaagaggatt ttatggataa aaaatggttg gatcaagtaa tcttttccaa caatccattg
    24721 gaactggtca atgaattacg agcagtatct attaaaaatc agttattatt gaaggaggca
    24781 aaataatgag tcaaatatat ttagcaggac catttttttc agaggagcaa atcgatcgag
    24841 taagtcgtat cgaaaaagcg ttggaggaaa acaaaacagt cacaagtttc tactctccac
    24901 gtcatcacca agaaagcaat tatgaactat tcagcgctgg atgggcgcag gaagtatacg
    24961 aaaaagatat ggaagaactg acaaatgcag aatttgttgt tgctatcctt gactttgagc
    25021 atcaaacaat cgatccagga acggcttacg aattaggtgt ggcaactatg ttgaaaaaac
    25081 cgatgatcat tgtacaagaa gagacggtac ctacgaactt gatgattaca caaagtctcc
    25141 atacgtattt aaaatcagac caagctgtac gtgaatatga ttttgaaaca ttgcctgttg
    25201 aaacttacgt aggcgagtac ttatagaaga ggaagggttg tatgtcagtg attttgattt
    25261 caggaacgat tggtgcaggt aagagcagtt tgacggatat gcttgcaaaa gagattgatt
    25321 ctaaaccatt ttatgaaaat gtcgaagata acgaagtatt gccgctcttt tattcaaatc
    25381 ctgagcagta cgctttttta ttgcaaatct tctttttgaa caagcgattt ttagcaatga
    25441 aaaatgcttt agtgaacgat gacaatgtgc tagaccgatc catctatgaa gatagcctgt
    25501 tgttccatct aaacgctgat ttaggtcggg taacggatat cgaagtacag cagtacgata
    25561 gcttattaga tacgatgcta aacgagttag atgatgttgc tccaaaaaaa cggcctgatt
    25621 tgatggtcca tatcaaagtt tctttggaca cgatgctaga acgtatcaaa aaacgtgggc
    25681 gtgactatga acagctagaa agcgatgaga cgttatatac ctattatgaa acgttgaaca
    25741 cccgctataa tcaatggtat gaagattttg atgtatgtcc taaaatacaa gtggatgggg
    25801 ataaattcga ttttgtagaa aatccagaag atgcaaaaaa ggtgatccgt cagatccaag
    25861 aaaaattagc tgaattggaa ggaaagcctg ctggaaaata ttttacggat aaccagcatg
    25921 caaaaggtca agaattaggc taaaaataaa aaaccaaacg acgggaaaga a
//
LOCUS       DACCCM010000040        25808 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCCM010000040 DACCCM010000000
VERSION     DACCCM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.041"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            7..849
                     /locus_tag="IVK78_001579"
     CDS             7..849
                     /locus_tag="IVK78_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6375056.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            846..1796
                     /locus_tag="IVK78_001580"
     CDS             846..1796
                     /locus_tag="IVK78_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6375057.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1811..2044
                     /locus_tag="IVK78_001581"
     CDS             1811..2044
                     /locus_tag="IVK78_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP6375058.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2375..2959
                     /locus_tag="IVK78_001582"
     CDS             2375..2959
                     /locus_tag="IVK78_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6375059.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3090..3758
                     /locus_tag="IVK78_001583"
     CDS             3090..3758
                     /locus_tag="IVK78_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6375060.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3760..5184
                     /locus_tag="IVK78_001584"
     CDS             3760..5184
                     /locus_tag="IVK78_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6375061.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5202..5771
                     /locus_tag="IVK78_001585"
     CDS             5202..5771
                     /locus_tag="IVK78_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP6375062.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5881..7635
                     /locus_tag="IVK78_001586"
     CDS             5881..7635
                     /locus_tag="IVK78_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375063.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7589..9361
                     /locus_tag="IVK78_001587"
     CDS             7589..9361
                     /locus_tag="IVK78_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375064.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9884..10153
                     /gene="rpsN"
                     /locus_tag="IVK78_001588"
     CDS             9884..10153
                     /gene="rpsN"
                     /locus_tag="IVK78_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6375065.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10168..10347
                     /gene="rpmF"
                     /locus_tag="IVK78_001589"
     CDS             10168..10347
                     /gene="rpmF"
                     /locus_tag="IVK78_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6375066.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10379..10756
                     /locus_tag="IVK78_001590"
     CDS             10379..10756
                     /locus_tag="IVK78_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375067.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10777..10926
                     /gene="rpmG"
                     /locus_tag="IVK78_001591"
     CDS             10777..10926
                     /gene="rpmG"
                     /locus_tag="IVK78_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6375068.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10950..11933
                     /locus_tag="IVK78_001592"
     CDS             10950..11933
                     /locus_tag="IVK78_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6375069.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11963..12085
                     /locus_tag="IVK78_001593"
     CDS             11963..12085
                     /locus_tag="IVK78_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6375070.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12103..13626
                     /locus_tag="IVK78_001594"
     CDS             12103..13626
                     /locus_tag="IVK78_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6375071.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13650..13889
                     /locus_tag="IVK78_001595"
     CDS             13650..13889
                     /locus_tag="IVK78_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375072.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14082..14543)
                     /locus_tag="IVK78_001596"
     CDS             complement(14082..14543)
                     /locus_tag="IVK78_001596"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6375073.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14738..14956)
                     /locus_tag="IVK78_001597"
     CDS             complement(14738..14956)
                     /locus_tag="IVK78_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6375074.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15074..16690
                     /locus_tag="IVK78_001598"
     CDS             15074..16690
                     /locus_tag="IVK78_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6375075.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16931..18271)
                     /locus_tag="IVK78_001599"
                     /pseudo
     CDS             complement(16931..18271)
                     /locus_tag="IVK78_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18547..18702)
                     /locus_tag="IVK78_001600"
     CDS             complement(18547..18702)
                     /locus_tag="IVK78_001600"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6375076.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18998..19330)
                     /locus_tag="IVK78_001601"
     CDS             complement(18998..19330)
                     /locus_tag="IVK78_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6375077.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19844..20035)
                     /locus_tag="IVK78_001602"
     CDS             complement(19844..20035)
                     /locus_tag="IVK78_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6375078.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20129..20515)
                     /locus_tag="IVK78_001603"
     CDS             complement(20129..20515)
                     /locus_tag="IVK78_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6375079.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21209..22414
                     /locus_tag="IVK78_001604"
     CDS             21209..22414
                     /locus_tag="IVK78_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP6375080.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22431..22865
                     /locus_tag="IVK78_001605"
     CDS             22431..22865
                     /locus_tag="IVK78_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375081.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23482..>25808
                     /gene="esp"
                     /locus_tag="IVK78_001606"
     CDS             23482..>25808
                     /gene="esp"
                     /locus_tag="IVK78_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP6375082.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         8896 a         3698 c         4830 g         8384 t
ORIGIN      
        1 aataatatgc ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct
       61 ctaattacat ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta
      121 agaggaatgt ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt
      181 tcttatattt taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct
      241 atcattatta cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt
      301 actttcagtt cttttttagc attaggagtt atccttatag gagttgcaaa tagctcaacg
      361 gatttatttc atatcttatt tggaaatatt ttagctgtac aagatattga taagtggatt
      421 acaattggcg tttctgtttt agtgttggtt acagttgttc tattttttaa agaattatta
      481 atcacttctt ttgatccatt aatggcaaaa gcaatgggga tgaatgtcag tttttatcat
      541 tatctattaa tgattctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact
      601 atattaattg ttgcaatgtt aattacacca gctgctacag catatttata tgcaaacagc
      661 ttaaaaacta tgatatttat ttcggcgaca gtcggagcgt gctcatcatt gctaggtttg
      721 ttcattggtt atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc
      781 ttatttgtaa ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga
      841 aagttatgat gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt
      901 ttatattcgg ggcatgttca acaaatagta aagacaaaga tacagtggct tcgaacgaaa
      961 aattaaaggt agtagttact aattcgattt tagcagatat tactgaaaat atagcaaaag
     1021 ataaaattga tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt
     1081 tgcctgaaga tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact
     1141 tggaaactgg aggaaatgct tggtttacaa aattagtaaa aaatgcgaac aaagaggaaa
     1201 acaaagacta ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg
     1261 agaaagggaa ggaagatccc catgcttggt taaatttaga aaacggtatt atttacgcta
     1321 aaaatattga aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa
     1381 atctagataa gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg
     1441 cttcaattcc gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct
     1501 cgaaagcgta taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa
     1561 caccagatca aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat
     1621 tcgtagaaag cagtgtggac gatagaccaa tgaaaacagt atcaaaagat accaatattc
     1681 ctatctattc aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt
     1741 actatgcgat gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg
     1801 gagggtaggt atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca
     1861 ggaattatca aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt
     1921 tagtccagca tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat
     1981 aggatttcaa ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa
     2041 gtaaaagact ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga
     2101 cttaccctaa tagctggata taattttttt aaatctttct cggattattt tagaaaaggt
     2161 gaatgattta gatgagtcta ttaattcttg taggattgct ttttgtatca gttattttgc
     2221 tctttctcat tagtttagct ctaaggaatg agttcagtaa tagacgttaa cgattgaaac
     2281 ttcaagtcct gtgtaagata atatagttaa ttcatcttat taggaggaag tacccataga
     2341 aaccagagat tatattacaa ctcaccaaaa attaatagaa agtggactaa gccattttga
     2401 aaaagaagga tttcagcaaa ctaagataag gcaactgtgc ttgaccgcag gcgtaactac
     2461 gggtgctttt tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
     2521 gcatattaat gcgcttatta atatgtataa tgaagagttt accaaatacc tcttaaactt
     2581 agaaaatata gatatagaga aaagttggag agagaacttc aatgaattag atccatttat
     2641 taattatatt tttgagaata aacgtatatt cgatttggtt ttattccaat cagctggaac
     2701 caaatatcaa gatattgttg aaaaaattac atcatttgtg acaaaaaaga cagtctgttg
     2761 tctagataag ttaaagacta tcggaatgat caatcaaaat ttagtcttta atgaagaaga
     2821 aatccacttc tacatttact cattctacgc tacattctat gatatattga agcatagcta
     2881 ttcaaaagaa aagactatgc tattaaccag aagattgtat caattttctg ttcctgggtg
     2941 gattgaatta ctgagttaat taaaaaaaag cttgcattga gaattgttct cagttatgct
     3001 ttttgataag atcgcactgt tatctgaata acggtgcatt tttgtcagct taagataaca
     3061 gtgcgatctt attttttgga ggtgaaagag tgtcaaaaat caatccgtta accttgatta
     3121 ctttaaatat tttttttccg gtcattattt ttttaggaaa gggaaatata tttcaagtgg
     3181 ggtgtttcat tattgctaca gttttagtat tgatgtttcg taaaataaag acatgtattt
     3241 atttcatctt gatttattca gttctgtttt tagtaatgaa aattattgat gctatcaaga
     3301 tagagttttt tactatattt tttgggacat ccttatatat tgtatttcga atgttgccgg
     3361 tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta ttatcctctt
     3421 tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg atcagattct
     3481 ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg agaaacattc
     3541 gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc gttcttatac
     3601 gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg atagaatctc
     3661 ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat atcatcctgt
     3721 tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt gtttgaattt
     3781 aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga ccttaatttt
     3841 tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg gaaatccaca
     3901 ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt ggttggggaa
     3961 attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc aaaaaatatt
     4021 ggctacgtaa gtcaaaatcc tagaggacaa ttctttacaa caaatacaac gagtgagtta
     4081 gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag aatcaatgaa
     4141 ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc gctatcaagt
     4201 ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa attaattata
     4261 ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc tgacttactt
     4321 tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt ttactatttg
     4381 aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt
     4441 cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt taatagttcg
     4501 attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga agaaattact
     4561 tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt tattggtttg
     4621 gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa gattattcgc
     4681 cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg
     4741 caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc cctggggaat
     4801 gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact ttataaaatg
     4861 aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt ggtttctata
     4921 gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg gttggattat
     4981 atcaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt
     5041 atagccactc atgatccgga atttctttat aaaacatgca atagaattat ttatttaaaa
     5101 aatggtggaa taaaaaaaga tataaaactt agagtagaag attcaacttt gatagaacag
     5161 atttttaatg acataacgaa atagaattga ggagaatata tatgaaaaca aaagattata
     5221 tttttattgg tttagctaca gttatttccc tagtaattta ctttttttca ataatggtta
     5281 gtagtattgg tggggcgttt ggtcacagca tatccccagg gatttttgga ttattatctg
     5341 gaatcatatt tgtttatatt agctataact accctagaaa aggaattttt actatttaca
     5401 caattgtttt attgttattt tttacgttaa tgggcggggc gtacttacct tggtttatca
     5461 gttcgattag tatggcgatc ttagctgata ttattctatc tgtatttgga tatgatcgag
     5521 caattcctca agttgtgtct tgggttctaa tgcaactagg cagtgctgcc ggacagtgga
     5581 ttcccatatg gttctttaca gatcgttttc gtcaagactg gatagataga ggacaatctg
     5641 ctgcaacaat ggatgcaatg atccactacg cggtgggtat atggggaatt atttcagtgt
     5701 tagttgtagc aagtttatct atgattggtg tattgattgg aagaaaagtc ttaaaaaaat
     5761 acaaaaaata gaacgtagtt taccttctat ctgtttgtac atctctaatt tacgctatta
     5821 cggaaagcat taacagttgt aaacgaataa gagaagaaat tgaatgcggg ggaggaaaaa
     5881 atgaaagtat acaaaaaatt atttagttat gtccctaaaa tgaaatacta cggatggtta
     5941 gccactttat tttctgattt ttctgtcgta ttaacagtct atggatatta cagtatttac
     6001 aaatttcttc agtctctaat tattgtagga gatgaatatt tagctaagag ttatgcggtt
     6061 caaactgttg cttggttaac tttaggagct ttattttata ttttttctgg cttattttct
     6121 catatccttg ggtttcgctt agagactaat ttgagaaaaa agggaattag cggtttaaca
     6181 gaagcaagtt ttcgtttttt tgatttaaat tcttcgggat atattcgaaa gacaattgat
     6241 gacaacgcgg ctaaaacaca tatggcaatt gctcacttga ttccagatag tgctcaagcc
     6301 atagtaacac ctgttttatc agttgcctta ggctttttca ttaactttaa aattggattg
     6361 gttctaatcg ccatgttagt aattggtgta ttcttgttaa agaaaatgac agagaataca
     6421 aattttattc aaaaatatca agaatcgtta gatgtgttaa gctctgaaac ggtagaatat
     6481 atccgcggaa ttcaagttat aaagatattt ggtgctaaag taacatcatt taaagccttg
     6541 aataaagcaa ttaacgatta tgctaagttt gcatataagt attctttgag tgggaaaaca
     6601 ccttttgttc tttttcaatg gctatttttt ggcatagttt gcattttgat agttcctata
     6661 acctttttta taacttctct taccactccc aagatcttag ctgttgactt gttgatgttg
     6721 ttttttttaa gtggaattat ttttgtttct ttcatgagaa taatgtatgt aagtatgcac
     6781 ttgtttgaag caagatatgc agtcgaaaca ctagactccc tttatttaga aatgaagcaa
     6841 gaccaactaa aatatgggaa cgagacacag attaaaaatt gtgacattgc atttgaaaat
     6901 gtcagctttt catacaaaga agagtgtgtt ttagaaaatt tcaacttagt tttagaacaa
     6961 aaaagaattt atgcattagt aggaaaatca ggaagcggaa agagtacgat agctaaacta
     7021 atttcaggtt tctacaatgt ggataaggga cagattaaaa ttggtggtaa gcctttaaat
     7081 cactattcaa aagacacaat tattaacaca attgcttttg tctttcaaga tacgaaacta
     7141 ttcaatcaga gtatttacga taacgtttct atagcaaatc ctcaagcgcc agcaagtaaa
     7201 gtatttgacg cgctaagatt agccggttgc gaatcgattt tagaaaaatt tcccgaaaga
     7261 gagcatacct taattggtag taaaggagtt tatctgtcag gaggagaaaa acaacgtata
     7321 gctattgcca gagcaatttt aaaagatgca aagattgtta ttatggatga agccagtgca
     7381 tctattgatc cagataatga gtatgaacta caaaaggctt ttcaatattt gatgaaagaa
     7441 aaaacagtta ttatgatagc tcatcgatta tccagcatta aatctgtaga tgaaattctt
     7501 gtcttgtcag aaggaaaaat agtagaacgt ggaaatcata atgaattaat ggctaaacat
     7561 cgagtgtata aacaattggt cactaattat gagaatgcaa atgattggag gcttgacaat
     7621 gaaaaatttt tataaaaaaa aatttgcttt aactgatcag ggcgcagaag ctttaactaa
     7681 agcttctatt tctagttttt ttgtatactg cattaatatg gtaccagcat tcattataat
     7741 gatgctgata gacgaattag ttttagaaaa tgcaaagccc cgttggcttt attttgcagt
     7801 atcgtttgtt actttgcttt tcatgtattg gttgttagat agggagtatg aaaacttata
     7861 taatagtact tataaagaaa gtgcgcattt aagagtgcaa attgcagacg atttgtcaaa
     7921 tttaccatta tcctattttt caaaacataa tttatcagat ttatctcaaa ctatcatgtc
     7981 tgacgttgaa ggtattgagc atgcgatgag tcatgcaata cctaaagccg gcggtatggc
     8041 tctgtttttc ccttttattt cagtgatgct tttggttggt aatgtcaaaa tgggattagc
     8101 tgttattttg ccaacgttat ttagttttgt cttaatcttg ttatcaaaga aatcccaaac
     8161 gaaagccaat actaaatatt acgatacttt gagagaaaac tcggaagaat ttcaagaaac
     8221 tattgaattg cagcaagaga ttaatagctt taatctatct aaaaaagttc aagacagact
     8281 tttcaaaaaa atggaagaga gtgaaaggat tcatttaaag gtagaattaa gtactttttc
     8341 agtcatggcc ttatcctcta ttttctcata tgttagttta gcagtagtca ttctagtagg
     8401 tgttcactta ctgttaacgg gtgaagtgac tatactctac gtcgttggtt acttactagc
     8461 cgcaataaaa ataaaggatt ccttcgattc tatgaaagaa gcagtgcttg aaatatttta
     8521 tttagctcct aaaatacagc gaattagagc tatgaaggaa accagtatcc aagagggatc
     8581 agatagtcct ctgaaatctt ttgacattga gttgcgagat gtctctttct catatgataa
     8641 caatacccca attttagatc atatctcttt tacggctaaa caaggagaag tgactgcgtt
     8701 agtaggtgct agtggttccg ggaaaactag tatcttgaaa ttagtgtcta gactgtatga
     8761 ttatgatgaa ggctgtattt taattgatgg gtatgatatt aaacgtgtat ctccagcttc
     8821 tttgtttagc aaaatcgcta ttgtttttca agaggtaact ttgtttaata caagtatttt
     8881 ggaaaatata cgtattggaa atagtcaagc gtcagatgag gaagtgaaaa aagcagctcg
     8941 tttagctaac tgcgaagact ttattgaaaa actacctgac ggttatcata cattggtcgg
     9001 agaaaacgga agttcacttt ctggaggaga gcgacaacgc ctatccatag cacgggcttt
     9061 tttgaaaaat gcgccaattt taattttaga tgaaattact gccagtttag atgcggaaaa
     9121 tgaaaaacgt attcaggaaa gtttaaatcg tttaatacaa gataagacag tactaattat
     9181 atcgcatcgc ttgaagtcta ttgaaaaggt aaataaaatt gtagtgatgg atcaaggaaa
     9241 actagttgat caaggtactc ataatgagtt gtatcgaagg tcagaaatat acaaaaatct
     9301 cataaagaaa actaaactat ctgagaaatt cgtctatgaa aaagaagctc aatcacgatg
     9361 atgaaatagg ctttaaatta acctacaatt tttgggatat agttataatt ctttttcaaa
     9421 aagaataatt ttattaaaca gcatctaatt ttgaatacac gaaagaatca gtatgattga
     9481 attagttcac ttaatgtcta ttcttataat ataaggaata atcaaagttt taaaaaattg
     9541 gcgataaacc agttaaacat aaaagattga ataaaaagtt atagtttctt tcatattgcc
     9601 gttttaagta gaggaaaagc tacttaaaac ggtatttgtg tttgattgga ctaataaaag
     9661 tgaattctta tttttcagag cctaaactgc atattctttt aaatgatttg attatctcta
     9721 tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat
     9781 ttattgttga caagagcaac tcttgttggt aaagtatcag tgtcaaaacg taacgattac
     9841 gatttgaggt gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa
     9901 aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tatgcagata aacgtcagga
     9961 attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa
    10021 tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg
    10081 tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa
    10141 agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg
    10201 aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa
    10261 gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag
    10321 aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat
    10381 ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca
    10441 gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga
    10501 aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga
    10561 ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga
    10621 ggagttattt caatgtatcg atggttattg ggagatacct gttgatatga aagaaaattt
    10681 agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata
    10741 tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa
    10801 tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa
    10861 cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt taaagaagtg
    10921 aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt
    10981 ttttaggggc tggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca
    11041 gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat
    11101 tagttcatag cgaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc
    11161 gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac
    11221 caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa
    11281 cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta
    11341 ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca
    11401 tggcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat
    11461 tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc acacatagtg
    11521 aaacgattca aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg
    11581 aaagtgattc atcagaaaat cactataatg gcaaatacta tcatcaacac catcaagtac
    11641 atgagtttaa atcaattctc ttaacttcag atcaagacat aaaaaaagat tttttactcc
    11701 aatggattga ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga
    11761 ttgctttaca agagcaggat tttatagttg ccatgcaaga ggtaaacgaa caggttaatt
    11821 ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg
    11881 atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag
    11941 tagtgattgg gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact
    12001 taaaagcccg aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg
    12061 aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta
    12121 ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca
    12181 aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat
    12241 gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt tccagcggga
    12301 tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat
    12361 gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg
    12421 aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt
    12481 gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg
    12541 tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa
    12601 ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg
    12661 aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg
    12721 acgcaacacg cagcctttgg atatttagct ttggattatg gattgaaaca agttcctatt
    12781 gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat
    12841 gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaattgca
    12901 aaaactttgg ccaacgaagc tgggataaaa ttggaagtac tgaatcctct tgaaagtctt
    12961 acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa
    13021 gcgttagaaa aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag
    13081 gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg
    13141 agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat
    13201 gaagttttcg attacaaagc aaaaatcaat aaagacatga cagctgccga gtacaaagat
    13261 tattatacta ctggctataa gacagatatt gatactatca acattaaaga caacaccatt
    13321 gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt
    13381 ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt
    13441 gctggtcgtt ttaaatatat tcaatttagt gatcatggaa ttgcaccaag taaggcagag
    13501 catttccata ttttctttgg tggagaaagt caagaaaaac tctataatga attgcataat
    13561 tggcctactt tctatccagc ttcattgtct aaacatgaaa ttgctcaaga aatgatggct
    13621 cattaaaatt caatttttgg aggaaataca tggtaagaaa agtattattg tctatggatt
    13681 ccaagaatat tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga
    13741 gtacattagc tgatattgct gcaactttac atgttagtat tgacacagtt catagagtat
    13801 taaaacggga tcctgattgt tgttttatca aggtcaatac tggaagaagt agacgtttag
    13861 caaaacggca taatagcttg gccaattaat tgataatacc agagtagatc atttttccct
    13921 tatttggatt tgatctgctt tttattatgt cttgaattgt caaatcaagc gcaacacttt
    13981 atcaaagaag acttcataag gtgttctcca atctaaacac tttctaggtc tgttattcag
    14041 tttttcaatc atctgattta tcgttgagtt atctaccgta gctatctctt tcgattttgg
    14101 caagtattct cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc aagggcatga
    14161 ggatctggaa aataaaaggg catattattc aatgcttctg taactgcgaa atgattggag
    14221 aattcttttc ctctatctgg agtgatcgtt ttgaccgcct catttggaat actgcttaaa
    14281 agttcaatca ttttctcgct cacataagcg gctttctttc tggggacctt ctttcctgct
    14341 aaaaggaagc atgattttct gtctgtcaaa gtcactaaac aagtaccacc agttttgcca
    14401 gctacaatat cggctttcca atgaccaaaa tcacttctat catctgcgga ttttggacgg
    14461 ccatggatcg aatgagtaag gcaaatcttt ccacgagact cgacatgatt ttttgtatgt
    14521 cttgtttttc ctcgatggtg caatgaacgg atcaggcctt tattgccgtg tgagaggggt
    14581 tctgtatcga aaagatcatt gcaaattgca cgttagatcg tgataaaact aattgaaaag
    14641 tccatatgtt catgcttcaa acgattcgag atttcctctg gcgaccactg aaaatcgaca
    14701 aataaatgac gaacaattga ccaaactcac ggatcattta gaatgtgata acgtccgcat
    14761 ttttttcttc gataagcata atttttttcc gctttggaag gtgaatagtt tttttcttgt
    14821 ttcttctcag ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg ccaattatac
    14881 taatagaaaa tccttcttcg tgaagcagaa agattctttc tcgttcgagt atagtaagat
    14941 gacgatactg gctcataatt attttccttc taggagtgtt ctcgacaaac ttatactagt
    15001 tggatatcga ttatgactca tttttatgtg ttgcacttaa agtgtaaatt caaggtatag
    15061 aaagaggttc cctatgaaaa aaatacctat tacaatctta actggctatc ttggttcagg
    15121 aaaaacgata cttctaaatc atattctgta cggtgatcac ggaaaggaaa ttgctgtcat
    15181 tgtaaatgac ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa
    15241 aagaactgga gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga
    15301 agatttgatt gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat
    15361 tgaagcttca ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa
    15421 ccctttagac attgatttga cccaacgatt agagattggt gccattatta tagtagtgga
    15481 tgtaaaacgt ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga
    15541 aggtaatgta gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg
    15601 ttgtaacata ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat
    15661 taacggtcta attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga
    15721 agtagactta tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc
    15781 agtgggctgg ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga
    15841 atatggtatc tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc
    15901 ccatcttacg gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt
    15961 tgcccaacat agtgattttt cgacgatctt tgaaacatcg ggtagctata aaagtattga
    16021 atcattaaat tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa
    16081 aaatcccgaa ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt
    16141 ttttattggt atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac
    16201 tgattctctt aatgcagata atttccgaaa aatttcacca tttccatgga aaaagtctaa
    16261 tatgtttagc cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga
    16321 tgtcataatt attggcattt tgattattac ctcttttatt cccttatttg tattctctat
    16381 agaaaaggag aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc gaattgatgg
    16441 caaggaaatg gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc
    16501 tgcgaaggga caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga
    16561 taatagtcct gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag
    16621 tatttgttta ccccataagt tagttattag tattgagaaa aaagaaagca cagattacta
    16681 catttactaa tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa
    16741 taatagcagg ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga
    16801 caacaggcac aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt
    16861 acttattttg gctcttcgtc aatgagtgtt ggtgaagaaa ttcaatagaa taatcgagat
    16921 actcacacgg ctagacagct atcttgtcta gccgtgttgt tgttctggct ttagtcagtt
    16981 gggtagtatt cttagttttc ttaaaatatt ttcctcgata taaaaagatt cgttctcggt
    17041 agttttgaaa gtttctatag ccataggcaa ttcgttttag tactttgata tggttattca
    17101 aacattctat agggccgttg gaatagggaa cgcgcaacgc tcgtttgatt tctgtttggt
    17161 atttcttgaa ggtcgtgaac actggttggt agtgctctgg caactccttg tagtcttctt
    17221 ttaggagcgc gacgaattca ggaacatctt gtcttctgat aacggaaaga aaatcctggt
    17281 agacttcgta gcctcgcttt aaggaatcat cataagataa tagccgatcg actatctctg
    17341 cttctgtcag atgatctcga aatgacggac gccagtagcg atgctgacct ttcaattccc
    17401 acgcattctt ttgaagtagt ttccagtatt tctttaagtg cttcgaccga cgatcccctt
    17461 ttttaaaggt cttcattact tgaattcggt ggttggtaaa agcccgacca atgtgttggc
    17521 taatatgaaa gcgatcggcg acgattcggg cattcggaaa acactctttc accaatgtgc
    17581 gatagggtgt atagaaatct gaaaccacta gacgcacttg ttcacggact tttatagggt
    17641 agcgcaagaa atagtcgcgt aaaggattga gttgccgatt ttccacaata tcaatcagtt
    17701 gatgggtctt gccgtccatc atgacaaagc tcatggctcc cgagacattt ttcactgatt
    17761 tgaattcatc gaaacacacc acttctggta acagctggct caaagaaatg gttttaggcc
    17821 gaaggctgcg aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca
    17881 aagacataga aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa
    17941 tggagcagcg acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac
    18001 atttgaagcg ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata
    18061 agatggatac ttttttccag ccctatttaa tgatcgtttg ttcattttga tagtggcgat
    18121 gaaagcattc ctccggttga taggtcaatg tgccatcaaa agcaaaacat gtttcgtcat
    18181 tgatgcgctc tttgcgaaag gcatcctcat gaaaggtaag atttaggtct agaatatcta
    18241 gtaactcttt ggtaaactct gaataggaca tcttgaaaca ctactttgaa tggtttttgt
    18301 cgactttatt ctaaaggatt ttaaggtgtc tgtttgcgtt tattagaaaa aagaaaaagt
    18361 attggcaaca gaaggcgtca ccaacactag atattataga tcccttattt tcttcgcttc
    18421 aaattgtcaa ggagtcatca taaattttgt atggattctt ttgtttgggt agtagtcttc
    18481 taaataatct tggattaaca ccttcatctc tattttactt ttatatttgt tggagtcgat
    18541 taattctcat ttgattgatc gatgaaacgc ttcaattact gcattgtcaa caggagtccc
    18601 agtttggctc actgaatgtg tgatgtggtg gtcgttcaac agctcttgat aaataaaaga
    18661 ccgatgctgg ctacctctat cggaatgaat taccgtaggc aattctcctt aataaatttg
    18721 taatggacgg acgacaatct ctttttttat gtgtgaatct accatatagt aacgtagaat
    18781 acgagttgct aagtcaatat aggtggacaa atagaggcga ccgtcattac atggaatgta
    18841 agtaatatcc atcacccaaa cagcatcaat ttggttctgt tcaaattatc gattcaacat
    18901 ttttttacca tttctttgat tgttttttct ctaccatgag aagatttgcg tcgatgaaat
    18961 cctcttgtgc ataaattcat ttcacgcata agaacagcta caacacgttt attgatttcg
    19021 attccttcat cgtaaagttt ttgagtgatc cctggagatc cgtatcgatc tttgtgttcg
    19081 tagaacactc gtcttcctct tttagtcgtt tattttcctg ttcaagaatg tagaaatctg
    19141 ctttatcagg taatcttttt ccttgtccag caaaagcatt tttcccgtat cgacgatatt
    19201 ctgaaaccca tcgggaaatt gttggacgag agatattgta ttcttcagca attttcttgg
    19261 tagttacttt tttctcaagg atatcctgtg aaatctttag tttaaattta cgatcatatg
    19321 tttttctcaa tagagttcat tccttccggt aacgtaactg atagtcaaga aaagtgtatc
    19381 atagcccaac ttagtatcag ggtttgtcaa ctaaactgtg gaagttgata gataagagtt
    19441 tttaacaacc aaaattctct tgactatttt tgaaatcaga caaattttga ttggacacat
    19501 agttgaataa atctaccgtc gttacggaag gtaaatcgca tgcttttcaa ttctagagga
    19561 gaagtcctcg ttgaagtacc atagcccagt tctggattcg acctcctgcc cacttcatac
    19621 gtaaataaag taattttagg agtgagactg tgacaaaagt tatttttaag ctaaaaacga
    19681 attaaaaaac gttgataatt aaaatcattc gttttagatt ctatgaatca ttatagaacg
    19741 ctgtaaaatc gatactttat aattataaag aagatagttt caaatttatt gttcgaaatt
    19801 tgaaactatc tttttactta tgtcacagac tcccttagtt gagttattct ttaatttcga
    19861 atttcgagat agctagatat attgcaagca tacattcttg ataacaatct tgatcatatt
    19921 ttccagttct ccaggattgc ttatgaatca aagagctaaa tcttttgaga attgcctcaa
    19981 cagcttcttc atttccagcc tttgctaaaa tgaaatcttc ttttaaagaa ttcataaatc
    20041 taccttcttc ccatggactt ataatctatt tctttgggaa ggaggagaac gagcaaagaa
    20101 gcatacaaaa ttagttatta tgcagatatt aaaccagcat tttttgaatt tttttgtaaa
    20161 gtcgatgttt taaatttgtc gcaccctgtc ttgtaatccc cagaaccttt ccaatttctt
    20221 tatcagtttt atcaaagata aatttttcga taagaaaaat ttgttcaggt tctttcaaca
    20281 aaggaaggat ttttgctaat tttatatctt caacaaggag agtcacattt aaaacattaa
    20341 tcggttgttt aaattttata gtatctttag taaacaaata tggtgggagt tgatcaagtt
    20401 gttcatgttt ttgaatttct tcttgttttt tataataagt attagcggtg ttcaaaatga
    20461 ccttttcaaa gaatcgaatc aactgggcat ttacgcgttc gtcgttatct gacatattgc
    20521 acacctcctc cattaagatt taaagggaaa agaggcaaaa aacacgaaaa gaagctaatg
    20581 caattatgct gcgctgcgaa taacttctat aagctcacat gcattgatag gactgctgat
    20641 gtttattttc aaaaatgttt ttttagttga taatggatag ttatctttat acttagaaaa
    20701 gaaaattcat atatcttctt aaatatcaaa catgtcctta tggctaagaa aatgtcttga
    20761 gctttttacg tataataaat cttttctaaa acctcctttt ggataatatt cttattcgtg
    20821 tatagattgt attttttatt tcttccccct tcataattaa caactagaaa agagttcgaa
    20881 tagacacgta tttgaacgaa aatttttatt ggattcccaa attggaatat ttagaaacga
    20941 aaaattccta ttatctgtat agtgcttaga taataacgga tcataataat tattgtcttt
    21001 gaactagata tatgtaaata gtattcatca tgatgaaaaa tgatgagttc tcggtgataa
    21061 aatgtacttt ttacggtgga atatacatga aagcgataag tggatatttt ataatgaagt
    21121 aagttagctt attttgacag aggaatagta ttttagataa cgtataaaat actatttaat
    21181 gatgtagaaa ggaaagagag aaacaaaagt gttttcagat aaagaagtct tattaaagtc
    21241 tcttcatgtt tgtttaaagt tacctatcgt ggtttttaat tccgaattca gtattataaa
    21301 agagtatcgc tcagatagaa ctacttattt tttttatgat tataaaaagt tactaactga
    21361 taatataaaa aaaactaaag atttccattt ctttactggt gattttaatg agatgtttct
    21421 tctatacatg tttaaaacca gatattactt atttggtcca tttagagcaa ataatattga
    21481 taaagttttt tttaaactaa aaatgaacaa tttgaatgtt gcaatggctg atagagaacg
    21541 actctataat tcattgcaaa atttgacttt gtattcttta ggagacattc gagatattct
    21601 gattttagtt cattattttt ttactggaaa aatagaagat ctattccatg aaccgttaat
    21661 tgaatataca gaaaatctaa gtaaaactat cgagcaaatc aaaatagata atctgttatc
    21721 acaaaattac gatccagaaa tatatctgtt tctttatgaa aataaaatac tggaatatgt
    21781 taaaaatgga gatattcgaa atttagaaaa catggttttt aatctgagta atggaataat
    21841 tccaagtgtt agtggagata cgattcggtc tgaaaaaaat tattctatca ttgtttttga
    21901 aaagttagca caaacgtcta taacattagg aatggatata atagaagcgt atcaaagtag
    21961 agatgctttg atacaagaaa acgaattagc tgtatcgtta cctgaggtct taaaagtgag
    22021 ggattctggg attgtttatt atactaagga aatcggaaaa actaagatag agcatctttc
    22081 tcccttaatt tcttctgtcg ttcaattcat cggcttaaat atctataaaa gaattactgt
    22141 aaaggaaata gctaattatt tttctgtaag tgaaactaaa ttaagagaaa gctttaagaa
    22201 tgaaatgcat attacgattt ataattatat ctctaagcga aaaatttcta cggctaaaat
    22261 aatgttgaaa tcaaaccata ctatcagtga agtttcttta ggtttaggat tttcagattc
    22321 ttcacacttt tcgagagtat tcaaaaagta cgcaggtgta tctcctaagc aatatcaatt
    22381 aggtttagtg gacaatttca acaacattag ctgagaggtt ttattttatt atgagactat
    22441 atcatttacg tagtttggag gagttgagga aattggattt taacaaaaaa tttttttggg
    22501 aaatttctga tttgagttgt ttcgtagaat ctgagcgtct aagtaatggt aataatagtg
    22561 tgttaaatga atatgacaaa gtagtaatta ataaagtaaa aagtttatgt gaagaagcta
    22621 gagaatctat cagtcatatt aatggtagca attttaaaca aacattattt aatctgatta
    22681 agctagatgc taaaatatca agttatttat tctttttgat acatgatgaa tttatggatt
    22741 atcaaaaatt ggatcagtta attgaaagag aaagctggga agattattac gtgggtctaa
    22801 ctttttcaga aaagttaaac aactacaagt taatagatta taagtatttg agtgaaagca
    22861 attaattatt catgtagagt actagacact ttaaatatgg acaattcaat tataaagaaa
    22921 aagaatcaat aatttaatga ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag
    22981 tgaaaaaatg ttgataactc caatcattcg tttaagattc taggaatcat tcagaaatgc
    23041 tgtgaaaccg atactttata attataaaga aggtgatttc aaattccgaa gaaggaattt
    23101 gaagtcacct ttttacttat gtcacagtct cattcagtaa tgcgggataa ggctccttat
    23161 tccgttattt gaatactctg aagtttaatc taagaaaggg taatattttg aaaacatgct
    23221 tcatcgcata gaaatgaact taatttcaga aagaatacat acatatcttt ttatatgttt
    23281 tcagctgctg aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt
    23341 ttttgttgta atgttcatga aattctctaa gtaaattagt taacataagg ttagtaagat
    23401 tcctattctg tgaataatac gtatcagatt tatcttttat tcttactagg aaagtaatta
    23461 ttaagggagt tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa
    23521 acaaagaaac gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt
    23581 ttgattggtg ttggtcttgt cttgggaaca actgaactag ttaaagcaca agatgaaatt
    23641 tctccaagca gcccacttga aacagcgata agctccgtac aagtaggtga caaagtagct
    23701 tcggggaata cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta
    23761 caatttagtc ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa
    23821 gtttacggaa aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta
    23881 gatgaacgat tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga
    23941 tcttctagac ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg
    24001 aaggtcaatt atattcgtgc aagtgatgga ttatttgctg gggcagaaac aaccgatacg
    24061 caaaccgctc ctaatggggt aattacgttt gaaaagagcc tagatgaaat ttttaaagag
    24121 ataggcatag acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat
    24181 caagacgatg ataaaattgt accgggtatt gatagtacgg gctacttttt aacagattca
    24241 gatgatttct ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga
    24301 tcagtaagtg caaaatatga gaaacctaat actcaaacaa aagatggttc aggatccaca
    24361 ggtgctaatg gagctataat cttagatcat aagttaacga aaaattataa tttctcatat
    24421 tcatcttccg caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt
    24481 ccatatgtag cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt
    24541 tcgtttgaat ccggaacaaa agtagctgat ttagcaatcg agcgacggga aggtcatgaa
    24601 aattatggca taggttcaat aactaataat gatttaacta aacttatcga ttttgctaat
    24661 gcaagtccac gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa
    24721 atcttagaag atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat
    24781 ttcatctttg attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga
    24841 acaggttatt attatttgca agatattgat ggtgatggaa accctgacga taaagaagag
    24901 agcggagaca cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac
    24961 acaacagtta aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa
    25021 cttgttgata aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat
    25081 ggggctccaa tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta
    25141 atcgcgaaag atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa
    25201 ggttcaaccg ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga
    25261 tttaaaccag atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag
    25321 tattcatgga aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta
    25381 actgttaaga tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct
    25441 caagctatgg aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt
    25501 caaagtggaa aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca
    25561 atttcagatg tctataataa ggacttcggt aatgcaagtg ttgatgcaaa cactggtatt
    25621 gttacattca ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct
    25681 attaaaattg tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt
    25741 aaagatattt atgaaaatcc aggagaaaac attcctgcag gctaccacaa agtaaccttc
    25801 accgcagg
//
LOCUS       DACCCM010000041        23655 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCCM010000041 DACCCM010000000
VERSION     DACCCM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.043"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            256..531
                     /locus_tag="IVK78_001607"
     CDS             256..531
                     /locus_tag="IVK78_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375083.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2622
                     /locus_tag="IVK78_001608"
     CDS             544..2622
                     /locus_tag="IVK78_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6375084.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2782..4668
                     /locus_tag="IVK78_001609"
     CDS             2782..4668
                     /locus_tag="IVK78_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6375085.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="IVK78_001610"
     CDS             4680..5627
                     /locus_tag="IVK78_001610"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6375086.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="IVK78_001611"
     CDS             5647..6174
                     /locus_tag="IVK78_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6375087.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="IVK78_001612"
     CDS             complement(6237..6890)
                     /locus_tag="IVK78_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6375088.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="IVK78_001613"
     CDS             7022..7864
                     /locus_tag="IVK78_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6375089.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="IVK78_001614"
     CDS             8022..8918
                     /locus_tag="IVK78_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6375090.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="IVK78_001615"
     CDS             8921..9634
                     /locus_tag="IVK78_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6375091.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="IVK78_001616"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="IVK78_001616"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6375092.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="IVK78_001617"
     CDS             10169..10396
                     /locus_tag="IVK78_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6375093.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="IVK78_001618"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="IVK78_001618"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6375094.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="IVK78_001619"
     CDS             complement(11182..11724)
                     /locus_tag="IVK78_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6375095.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="IVK78_001620"
     CDS             complement(11961..12359)
                     /locus_tag="IVK78_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6375096.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="IVK78_001621"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="IVK78_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6375097.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="IVK78_001622"
     CDS             13472..14239
                     /locus_tag="IVK78_001622"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6375098.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="IVK78_001623"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="IVK78_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6375099.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="IVK78_001624"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="IVK78_001624"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6375100.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="IVK78_001625"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="IVK78_001625"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6375101.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18895..19800
                     /gene="xerC"
                     /locus_tag="IVK78_001626"
     CDS             18895..19800
                     /gene="xerC"
                     /locus_tag="IVK78_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6375102.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19837..20385
                     /gene="hslV"
                     /locus_tag="IVK78_001627"
     CDS             19837..20385
                     /gene="hslV"
                     /locus_tag="IVK78_001627"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6375103.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20399..21799
                     /gene="hslU"
                     /locus_tag="IVK78_001628"
     CDS             20399..21799
                     /gene="hslU"
                     /locus_tag="IVK78_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6375104.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21817..22608
                     /gene="codY"
                     /locus_tag="IVK78_001629"
     CDS             21817..22608
                     /gene="codY"
                     /locus_tag="IVK78_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6375105.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22690..23566
                     /locus_tag="IVK78_001630"
                     /pseudo
     CDS             22690..23566
                     /locus_tag="IVK78_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
BASE COUNT         8192 a         4079 c         4983 g         6401 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcattttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacaccgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag
    18781 gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca atttatttgt
    18841 ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga gaaaatgaca
    18901 gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg atattcagaa
    18961 aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt ggaggaatct
    19021 ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt gagtgcatta
    19081 gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc cagcctacgt
    19141 tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt ttcttacgtt
    19201 catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga aatgcaagca
    19261 ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc tttactggaa
    19321 gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt atcagacatt
    19381 gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag atatgctcct
    19441 ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg ccaggtattg
    19501 atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg aaaaccaatc
    19561 acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag tttggatagt
    19621 gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa taatggggca
    19681 gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac ccagatctat
    19741 gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa
    19801 aaataaggaa aagaacaaat ttaggaggaa acaaacatgg tcgaatcacc atttcattca
    19861 accactatct gtgccgtcga aaaagacggg aaatttgcta tggctggaga tggccaagta
    19921 acaatgggtg aatctgttgt catgaaaggc actgcaaaga aagtacgacg tatctacaat
    19981 ggagaagttg ttgtaggttt cgccggaagt gttgcagatg cgtttacttt agaagaaaaa
    20041 tttgaaggca aactaaacga atataacggg aatctacaac gcgcagctgt cgaattagct
    20101 caagaatggc ggacacagca atctatgcaa aaacttgaag cgatgctgat cgtaatgaat
    20161 gataaagaaa tgcttcttgt ttcaggtaca ggagaagtta tcacaccaga tgatggtatc
    20221 ttagctatcg gatcaggagg gaattttgcg ttggctgcag cccgagcaat gaaaaactat
    20281 gctcaaaacg acatgagtgc gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat
    20341 atctgtatct ttactaatca taatattatc gtagaagaac tgtaagaggt gagtagatat
    20401 gtatgaatta aaacaaacac caagacaaat cgtaagtgaa ttagatgaat atatcgtagg
    20461 acagcacgca gcaaaaaaat ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca
    20521 gttagatgaa aaaatgcaac aagatattac accaaaaaat atgctgatga tcggacctac
    20581 aggggtcggt aaaacagaaa tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt
    20641 caaagtcgaa gcaacaaaat ttactgaagt cggttatgtc ggaagagatg tagaatcaat
    20701 ggttcgggat ttagtagaaa atgcgatcca aatcgtagaa aaagagcagt acagccgtgt
    20761 atacacaagt gctaaaaaaa atgcagaaaa acgtttggta aaaatcttgg tacctggtat
    20821 caaaaaagaa cagaaacaat ccactaatca atttgaacaa atgatgaatc tttttaatgc
    20881 tagtcagcaa aataccgctg aaccgcaaga agaagtgaca gaagatattc gaatcaatcg
    20941 ccgtacagtc ttggaacaac tacaaaaggg tttactggat agtcgtgaag taaccattga
    21001 agttgaagac caaaagaaac ctgcaccaat gatgaacaat ggattggagc aaatggggat
    21061 cgacctaaat gaaactctag gtgctttgac accgaagaaa aaagtggaac ggacggtaac
    21121 agtcaaagaa gctttagagt tattgatcaa ggaagaatca gcaaaattag tcaaggatgc
    21181 tgatatccat agtgaggcaa tcaaattagc ggaagaaagt ggaattattt ttatcgatga
    21241 aatcgataag atcacttcta aatcacagca atctggagaa gtatccagag aaggcgtcca
    21301 aagagacata ttgccaattg tcgaaggttc acaagtaaac acaaaatacg ggatcatcca
    21361 aacagatcac gtcctattca ttgcttcagg agctttccat ttgtctaaac caagtgatct
    21421 aattccagaa cttcaaggac gcttcccgat acgggtagaa ttagatgatt tgacagctga
    21481 agattttgtg aagattctga ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt
    21541 gatcggtaca gaaaatgtgt cagttacgtt tacaaaagaa gcaattgaac ggatcgctca
    21601 aatcgctttc gatgtcaacc gtgacacaga taatatcggc gcacgcagac tgcataccat
    21661 tttggaaaaa ctactagaag atctattatt tgaagcacca gatatgcaaa tgggagaaat
    21721 aacgatcaca gaagcatatg tggatgacaa acttggctca attgcggcaa atgaagattt
    21781 aagtcgctat attttataaa aaatcggagg acgacaatga cagacttgct aacgaaaacc
    21841 cgcaagataa acacactttt gcagcaaaag aatacttttg acatgcaagc agatttgcct
    21901 tacgataaaa tggctgtcac actaggtggt attcttgaca gcaacaccta tatcatagga
    21961 agtgacggag tattactagg ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa
    22021 acaatgtttg ttgagaaaca atttcctgaa tcttatactg atacagtggc acagttgatg
    22081 aaaactgaag ctaatatccc aattactagt gatattactg catttccggt agagtcaaga
    22141 gagaaatatc catttggtct gacgactgtc gttccgatct ttggagctgg cgaacgtttg
    22201 ggtacgatta tcttatctcg tatggaacaa tcgtttgcag atgacgatct agtcttagca
    22261 gaatatggag ctacggttgt cggtatgcag atcctttatc agaagtctcg aagcatcgaa
    22321 gcagatgtac ggagtgcaac ggctgtccaa atggcaatca acacattatc ttatagcgaa
    22381 ttgaaggctg tacaagctat ctttgaagca ttggatggag atgaaggtcg tctgactgct
    22441 tctaatattg ccgatagtat cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa
    22501 ttggaatcag ctggaatcat tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa
    22561 gtattgaatt cccgttttaa agacgagtta gcaaaacaca gttattagaa gtaaggggac
    22621 tggggcagat agaaatgttt gttccagcct tcccatttta aaaagtaagt cgaagttgga
    22681 ggaatggcaa tgactgttat tattcaaaat gaccagctga tcgccgaaat cgctgaacac
    22741 ggtgcggaat tgatcagtct gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt
    22801 gatccagcgt attggggcag acatgcaccg gttttattcc cttttgttgg acgtttaaaa
    22861 gatgatcagt atgtttatca agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg
    22921 gatatggatt ttgaagtcat tgaacatgga caagaaatgg ctagtttcat cttaaggagt
    22981 acgccggaga cattagaaaa gtatccattt gattttgaat tagtgatcag ctatgaatta
    23041 ggaggagacg gcatcacggt tcattaatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcg
//
LOCUS       DACCCM010000042        23576 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCCM010000042 DACCCM010000000
VERSION     DACCCM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.044"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            43..675
                     /locus_tag="IVK78_001631"
     CDS             43..675
                     /locus_tag="IVK78_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6375106.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(677..1582)
                     /locus_tag="IVK78_001632"
     CDS             complement(677..1582)
                     /locus_tag="IVK78_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6375107.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1667..2320)
                     /locus_tag="IVK78_001633"
     CDS             complement(1667..2320)
                     /locus_tag="IVK78_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6375108.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            2642..3586
                     /gene="mvk"
                     /locus_tag="IVK78_001634"
     CDS             2642..3586
                     /gene="mvk"
                     /locus_tag="IVK78_001634"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP6375109.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3579..4556
                     /gene="mvaD"
                     /locus_tag="IVK78_001635"
     CDS             3579..4556
                     /gene="mvaD"
                     /locus_tag="IVK78_001635"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP6375110.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4569..5654
                     /locus_tag="IVK78_001636"
     CDS             4569..5654
                     /locus_tag="IVK78_001636"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP6375111.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5651..6715
                     /locus_tag="IVK78_001637"
     CDS             5651..6715
                     /locus_tag="IVK78_001637"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP6375112.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6964..7767)
                     /locus_tag="IVK78_001638"
     CDS             complement(6964..7767)
                     /locus_tag="IVK78_001638"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP6375113.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7787..8629)
                     /gene="kduI"
                     /locus_tag="IVK78_001639"
     CDS             complement(7787..8629)
                     /gene="kduI"
                     /locus_tag="IVK78_001639"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP6375114.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(8645..9652)
                     /locus_tag="IVK78_001640"
     CDS             complement(8645..9652)
                     /locus_tag="IVK78_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP6375115.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(9659..10303)
                     /locus_tag="IVK78_001641"
     CDS             complement(9659..10303)
                     /locus_tag="IVK78_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6375116.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(10403..11161)
                     /locus_tag="IVK78_001642"
     CDS             complement(10403..11161)
                     /locus_tag="IVK78_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP6375117.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(11288..12439)
                     /gene="fucO"
                     /locus_tag="IVK78_001643"
     CDS             complement(11288..12439)
                     /gene="fucO"
                     /locus_tag="IVK78_001643"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP6375118.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(12521..12832)
                     /gene="rhaM"
                     /locus_tag="IVK78_001644"
     CDS             complement(12521..12832)
                     /gene="rhaM"
                     /locus_tag="IVK78_001644"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP6375119.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(12834..13679)
                     /gene="rhaD"
                     /locus_tag="IVK78_001645"
     CDS             complement(12834..13679)
                     /gene="rhaD"
                     /locus_tag="IVK78_001645"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP6375120.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(13693..14949)
                     /gene="rhaA"
                     /locus_tag="IVK78_001646"
     CDS             complement(13693..14949)
                     /gene="rhaA"
                     /locus_tag="IVK78_001646"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP6375121.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(14946..16406)
                     /gene="rhaB"
                     /locus_tag="IVK78_001647"
     CDS             complement(14946..16406)
                     /gene="rhaB"
                     /locus_tag="IVK78_001647"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP6375122.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            16559..17428
                     /locus_tag="IVK78_001648"
     CDS             16559..17428
                     /locus_tag="IVK78_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6375123.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(17554..18453)
                     /locus_tag="IVK78_001649"
     CDS             complement(17554..18453)
                     /locus_tag="IVK78_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6375124.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(18618..19442)
                     /locus_tag="IVK78_001650"
     CDS             complement(18618..19442)
                     /locus_tag="IVK78_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6375125.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(19453..20748)
                     /locus_tag="IVK78_001651"
     CDS             complement(19453..20748)
                     /locus_tag="IVK78_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP6375126.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(20764..21243)
                     /locus_tag="IVK78_001652"
     CDS             complement(20764..21243)
                     /locus_tag="IVK78_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP6375127.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(21249..22277)
                     /locus_tag="IVK78_001653"
     CDS             complement(21249..22277)
                     /locus_tag="IVK78_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP6375128.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(22294..23400)
                     /locus_tag="IVK78_001654"
     CDS             complement(22294..23400)
                     /locus_tag="IVK78_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6375129.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
BASE COUNT         6779 a         5200 c         4239 g         7358 t
ORIGIN      
        1 cccgcttctt tgctatacta aagaaaaacg aggtgaaccc acatgaaaaa aatcgaacct
       61 attccacctt ctcttcctct tcttacaaag ggagagtttt atttagaaga tgaggagatc
      121 ttctctggtc ttctgatcaa cgggggtgac caaagctatc tcgatgccaa aaatatggta
      181 ttaagagaga gtcatatcca aaaacttacg atgcaaaaaa cagttttacg tcattttgaa
      241 tgcagtaatg tcatttttga aaaatgtgat ttttccaatc tggagtggat tggtggcagt
      301 ttccaccaag tcgtgtttca tcaatgtaaa ttaacaggta cgaattttgc cgaaagttat
      361 cttcgagact gtactttcac tgattgtatt gcttcgatgg cttcctttag tagtacaaac
      421 ttgaaagctg tctccttcga tcattgtcaa ttagaagaca gcgaatttta tgaggtcact
      481 tggaaaaacc tttttttgtc tgacaatcag ttgaccggtt cgaactggtt tcgaacttct
      541 ctgaagggat tagactttac gactaatttc ttcacagcaa tcgctttatc tcaagattct
      601 ttggcgggac taatcgtcaa ccaagagcaa gcacttgtga tcgcccaagc ccttggtttg
      661 cagatcaaag actagatcaa cgaatccgcc gccacagtgc aataagcatc caaatagata
      721 agaccaaact gatgatcaag gcgattcccc atccgaaaac ggaatgggta aagggtaact
      781 ggagattcat tccgaagaat cctgtcacaa tagttggaac ggtcaagatc agcgaccaga
      841 ccgtcaaaaa tttcatcgtg tcattcaagt tgttattcag caaattatta taagtgccag
      901 agagctggtc taaaatctgc gaagctaagt tcgtcatttc aactgcttgt cgtgcttcga
      961 tcagcgcatc gtccaactct tctttttcct tttctgacaa cttgcgataa atagccaatg
     1021 ccttgatttg ttctaaaagt actacatttt gttttgttcc tgtaactaag taaaccaaac
     1081 cgatctctaa atctgaaagt tgcagcagat tcttgttcgt tgttttttct cttaatttca
     1141 gattaagccg ctgacgttca ctattcactt cctcgattaa aggaaaaaag gaatcagaaa
     1201 tcaaaaacaa tgtacgaaac aataaactgt atacactcat gtctggcatt cgttccaaaa
     1261 gcgatcgaat tagtcgaatc acgtactcgg tattcggcgt ggtaaatgta aacaaaccgt
     1321 cttcttttaa aataaaagcg ataggactag tttcataatg attatcgact ttttgctggt
     1381 gcggaacatt tgatacaagc agtaacgctt catcaaaaga atcgtactct acgcgcgccc
     1441 gttcattttt atctagagaa taagtcagca tctcgtcact aatgccatat tcttctttta
     1501 agtttttaac agctagcaca tcgttttgct gtacattgat ccaaaaatta cggtcttgat
     1561 taaaatagtg tttttcccgc atactctatc ctcctgacat ttgtttccag tataaagaaa
     1621 aaagtcgctc aaataaaggt ttacccttta tttgagcaac tgtaaatcaa atggctactt
     1681 tggtttggcg aatctctgct aatttttctt cgatcatttt caagacttct tcttttgctt
     1741 gaggatcttc cacaaaatta tagcggtctc catcgatttg gatttttgga ctttctttgt
     1801 attcttcata ccattgatcg taacgtctgt tcaattcttt gtaataatcg tatagacttg
     1861 gatcaaagga caattgttca tacgaacgac cacgtttttc gatacgctcc aacatcgtat
     1921 caaaagatac acgaatatga actaataaat ccggatgttt tttatgggca gcatagggaa
     1981 gttcttccat catgttttcc agcagttcat catacacacg aacttctgtt tctgttgctc
     2041 ttcctagatc tgcatttaga tggaaaagca gtgaatcttc ataaatcgaa cggtccaata
     2101 cattattgtc atctttcatt gcttgtttga tgcttgcaaa acgtttattc aaaaaataaa
     2161 tttgcagcaa aaaagcatac tgttcagggt ttgcataaaa taagggcaag acttcattat
     2221 catcaatcga ttcataaaat gcctgactat caaagtgttc agccatcatt tctgttaaac
     2281 ttgattttcc ggcaccaatt gtacctgcta aaacgatcac gctcatttac ctccagctca
     2341 cttcatcact aaatattgtg tttaactcca acctttttaa atataccccc aatatctagt
     2401 agtgtcaatt gtattttttt ctagtcaaag aagaaaaaaa gtcaaaaaat tcttaagaaa
     2461 aaagtgtttt ttctttagaa aacgtattct tagttttact tctgagatgg ttttagtaaa
     2521 ataaaggagg aatgtttttt gagaccctta gtggacaaaa aaattcgtga agcagcgcaa
     2581 ctcgttttgg gcgctttatt ttttgcttaa aaatcacttt tacgtgagaa ggagttagat
     2641 tatggcaaac tatggccaag gagaatcaag cggaaagatc atattgatgg gcgagcacgc
     2701 ggttgtttat ggagaaccag cgattgcctt tcctttctat gcaacaaaag tcaccgcatt
     2761 ccttgaagag ctggatgcaa tggacgatca actggtttct tcctactatt caggaaattt
     2821 agccgaagct cctcatgcat taaaaaatat caaaaaatta ttcattcact taaaaaaaca
     2881 gcatgacatc caaaaaaact tgcaactgac cattgaaagc acgattcctg ctgaacgtgg
     2941 aatgggatca agcgctgcag tcgccacagc agtcactcgt gctttttatg attacttagc
     3001 atttcctttg tctcgtgaaa tactattaga aaatgtccag ctttcggaaa aaatcgccca
     3061 cggtaatcct agtggaatcg atgcagccgc tactagcagc ttgcagccga tttattttac
     3121 aaaagggcat cctttcgact acttttcttt gaacatcgat gcttttttga ttgtcgctga
     3181 tacaggaatc aaaggacaaa caagagaagc cgtcaaagat gtcgctcacc tctttgaaac
     3241 ccagcctcat gaaactggac aaatgatcca aaaattagga tacttgacga agcaagcaaa
     3301 acaagcaatc attgaaaatt caccagaaac gttggcacag acaatggatg aatcacaatc
     3361 acttctggaa aagctgacaa tcagcaatga ttttcttaat ctattgatcc aaacagcaaa
     3421 agataccggg gcattgggtg ccaaattaac tggcggtgga cgcggtggct gtatgattgc
     3481 attagcacaa acaaaaacaa aagcccaaga aatcagtcaa gcacttgaag atgcgggtgc
     3541 tgctgaaact tggatacaag gattaggagt acatacctat gtttaaaggc aaagcacgcg
     3601 catataccaa cattgctcta atcaaatatt ggggtaagaa aaatgaagaa ctcatccttc
     3661 caatgaacaa cagcctttcg ttaacattgg atgcatttta tacagagaca gaagtcatct
     3721 tttctgatag ctatatggta gatgaatttt atctagatgg caccttgcaa gacgaaaaag
     3781 caacaaaaaa agtcagtcag tttcttgacc tttttcgtaa agaagccggc ctctcactaa
     3841 aagcttcagt aatcagtcag aatttcgttc ctaccgcagc tggattagcc tcctctgcca
     3901 gcgggctagc tgctttagca ggcgcttgca atactgctct taagcttgga ttagatgatc
     3961 tctctctttc aagatttgct cgacgcgggt ctggttcagc ttgccgaagt attttcggtg
     4021 gtttcgtcga atgggaaaaa ggccatgacg acttaagttc ttacgctaag ccagtccctt
     4081 ccgattcttt cgaagacgat ttagcaatgg ttttcgtttt gatcaacgac cagaaaaaag
     4141 aagtgtccag cagaaatggg atgcgtcgga cagtcgaaac atccagtttt tatcaaggct
     4201 ggttagattc cgttgaaggg gatctatatc aattgaaaca agcaatcaaa acaaaagatt
     4261 tccaacttct cggagaaacg atggaaagaa acggactaaa aatgcatgga acgactttag
     4321 ctgcccagcc tccattcact tactggtctc ctgacagctt aaaagcgatg gatgcggtgc
     4381 gacaattgag aaagcaaggc attccatgct actttacgat ggatgctggc cctaatgtca
     4441 aagttttagt ggaaaacagc catttatctg aggtacaaga aacatttacc aaattattta
     4501 gcaaagaaca agtaattact gcacatgctg gtccaggaat tgcgattatt gaataaaggg
     4561 agatttttat gattgaagta tctgcaccag gcaaactcta tattgccgga gaatatgcag
     4621 ttgttgaaac aggccatcca gcagttatcg ctgcagtcga tcaattcgtg acagtcactg
     4681 tagaatctgc acgaaaagtc ggaagtatcc aatctgcaca atatagtggg atgcctgtac
     4741 gttggacaag acgcaacgga gaattagttt tagatattcg ggaaaatcct tttcattata
     4801 tccttgctgc tattcgcttg actgaaaagt atgcacaaga aaaaaacatt cttttatcct
     4861 tttatgatct gaaagtaaca agtgagttag acagttcaaa tggccggaaa tatggattgg
     4921 gttctagcgg tgccgtgacc gttgcaacgg tcaaagcctt gaatgttttt tacgcgttga
     4981 atttatctca gttggagatt ttcaagattg cagcactagc caatttagca gttcaagata
     5041 atggttcctg cggcgacatc gctgctagct gttatggtgg ctggatcgct ttctcaacct
     5101 tcgaccatcc ttggctccaa gaacaagaaa ctcagcattc tatcagtgag ttacttgccc
     5161 tggattggcc aggtctatcc attgagccat tgattgctcc tgaagattta cgtttattga
     5221 ttggttggac gggtagccct gcctctactt ctgatttggt cgatcaagtt caccgttcga
     5281 gagaagataa aatggtggct tatcagcttt tcttaaaaaa tagtacagaa tgtgtcaacg
     5341 aaatgatcaa agggtttaaa gaaaataatg taacgttgat ccaacagatg attcgaaaaa
     5401 accgacaatt actgcatgat ttatctgcaa tcactggggt cgtcatcgaa acgcctgctt
     5461 tgaacaaatt gtgtaattta gctgaacagt atgaaggagc cgcaaaatct tctggtgcag
     5521 gtgggggcga ttgcggaatc gtaattgttg accagaaatc tggcattctt cctttaatga
     5581 gtgcatggga aaaagcagaa atcactccac tgccgttaca tgtctatagc gatcaaagaa
     5641 aggaaaaccg atgaatcgaa aagacgaaca tgtttcacta gctaaagcct ttcatgataa
     5701 acaaaaaaac gaatttgact tcgtacggat catccataac cctttgccgc aaatagcagt
     5761 ttctgatgtc gatctaagta cacaggcagt tgggtttaca ctaagcagcc cattttatat
     5821 caatgcgatg actggcggaa gcgaaaaaac aaaaaaaatc aaccaagatc ttgcaatcgt
     5881 cgcaagagaa gcagatttga tgattgccac tggttcagtc agcgctgcct tgaaagtccc
     5941 ttctttagcc gatacttata cgattatgcg acaagaatat cctcacggga aaatcattgc
     6001 caacatcggt gcagggactt ctgtcgaaag agccaaagaa gctatccgat tgtttcacgc
     6061 agatgcctta cagattcatt tgaatgcacc tcaagagttg gttatgcctg agggagaccg
     6121 tgattttacc aattggaaag tacttattca agaaactcaa acagccattg acgtccccct
     6181 catcgtaaaa gaagtcggtt ttggcatgac aagagaaacg ctcaatgatc tagctgcttt
     6241 aggagttcat acagttgata tcagcggtcg aagcggaaca agcttcaccc agatcgaaaa
     6301 cgcccgtcgc tccaagcggg aactgagtta tttagctgac tgggggcaat cgacagtctc
     6361 ttcattgctt gaagcaaatg aagcagacac ttctatggaa atcctagcct ccggaggtat
     6421 ccgcaatgcc tatgacatat ttaaagccct ttgtctcggc gcaaatgccg tgggtacttc
     6481 aggaaccgta ctgacgcatc tgatgaatca cggcgtagaa gaaactatta tactgatgaa
     6541 gcaatggcaa gaagagcttc gtctgcttta tactatggtc ggcgcaacga ataccgcggc
     6601 cctacaccaa caatctctga tcttttccgg accagtgaag gattggtgcg aagcaagagg
     6661 gatcgactta gtgaagtatg gaagacgcac agaaaagagc gtgggacaaa agtaaagaac
     6721 acttttgtct cacgctctaa acacgtacaa acggcggaag taaaagcaac tccttcggaa
     6781 ataagctgaa attcacaaaa atttgaaaag caattttcgt gaattccttc ttatttcttg
     6841 gagttaaatg cttctgtccc agcctctttt ttcgaataaa tgatgtttaa tcagctgtcc
     6901 ttcggtattc gttcaatccg ctgatttcac aacctctcca ctaaacagtg tactaaagct
     6961 cttttaaggt tgttttccga tataggcaag tatgcctccg tccacataca agacatggcc
     7021 gttaacgaaa tcagatgcgc tagaagccaa gaagacagat ggtccagcca aatcgattgg
     7081 ctcgccccag cgtgctgctg gcgtacgtcc aacgataaat tgatcgaatg gatgacgttc
     7141 accgttttct tgtgtttcgc gtaatggagc agtttgcggt gtagcaatat aacctggtcc
     7201 aattccatta cattggatat tgtattgacc gtattcagaa gcgatgtttt tcgtcagcat
     7261 ttttaatccg ccttttgccg cagcataagc actgaccgtt tcgcgtccta attcactcat
     7321 catggagcag atattgatga ttttaccgcc gcctttttcg atcatatccg gaataactgc
     7381 ttttgaaacg atgaatgggg catttaaatc cacgtcgatt acttggcgga agtctgccgc
     7441 atccatttcg atcatcggtg tccgtttgat gatccctgca ttgttgacta gaatatcaac
     7501 tgaacctaca tcttctttga tctgttgaac catcgcttga accgcttctt catcggtcac
     7561 atcgcagaca taaccttttg catcgatgcc tgcttcttta taatgcgcta taccttcatc
     7621 aactgattct tgattcaagt tattaaatac gatttttgct cctgctgtag ctaaagagcg
     7681 tgcaatctca aatccaatac catagacagc accggtgact aatgctactt ttccatctaa
     7741 acgaaacata tccatactaa attccatcat tttctctccc ttatgcttat tttaattgat
     7801 ccatttgcac catatccatg tctgtatagg tgatattttc tccgcacatt gcccagatga
     7861 atgagtaatt gcttgtacct acacctgaat ggatcgacca gcttggcgaa ataatggctt
     7921 gttcattgtc tacaactaaa tgttttgttt catctggttt gcccatcatg tggaacacac
     7981 gtgtatcttc attggcataa tcaaagtaga cataagcttc catacgacgt tcatgtgtat
     8041 gacaaggcat cgtattccaa gcgcttcctg gttctaaaat cgtataaccc atttgtaatt
     8101 gacagctttc gcatacattt ggatggatgt attggtagat tttacgttca ttcagtgtca
     8161 ggccttcacc agtctccatt ggttttactt ggtcgatact gatttttaca tttggatatt
     8221 tatgatgagc tggaacagaa ctaatataga atttagctgg gttttcagca tcttgagaag
     8281 aaaatacaac atgtcgtgtt tcttttccga tatagtatcc gtcttgtttt ttcatttctt
     8341 ctttttgccc atcgatctcg atgaatcctg gtccaccgat attgatcaca cctagttcac
     8401 gacgctctaa gaagtaatca acacctagtt ctttgtctaa tgtgatttct aattcttttt
     8461 cagtaggtgt cactccacca aagatcattc gatcgttgtg cgtataagtc aaactgattt
     8521 tccctggttc aaataccttt tctactaaaa attcttttct caattgttcc gttgaataat
     8581 gacggatatc ttctggactg tgtgtataac gtgtttccat ttcttgcatt ttcttctact
     8641 ccttctatcg aatgattttt ccagagccgc atgctaaaaa ttgtgcgact tcttcttgac
     8701 taaattggtt gcagtcccca tggatcgtat gcttcaaggc agaagctgcc gtagcaaaat
     8761 caatgatttc ttgcggagac atattggata aaatcccatg gagcacgcca cctgcaaaag
     8821 catctccgcc acctactcga tccacgattg gggtgattct atggacttgg gattccacat
     8881 attcatggtt catccacaat gtccccttca attcattttc acttgctgaa tgaacggtac
     8941 gcttggtcga ataaaacacc tgaatattcg ggaacttttc ctgcattttt tgataataat
     9001 aaattagagg ttgttcttct gcttctggcg ctttctcgat acctaacaaa taaagagcat
     9061 ccatttttcc agctgagcag atatctacta aaggtaaaag ctgatggatt acttcgctgg
     9121 cttcttgctg tgtccataat tttccacgat agttgatatc gaagctgatc aggcagccag
     9181 ctttttttgc agcttctatc acctgtttcg tcattttttg ccatttttta gacaaagctg
     9241 gggtgattcc tgaaacatga aatatatcta ttccttggaa taactcctct agcgaccatt
     9301 ccaaatgatc catttcagca aaacttgatc cggcacgatc atagatcact gaggcggcgc
     9361 gttcgccact accagtttcc atataataag tacctagtct tgatccacca gataatacct
     9421 gtttcgtatc tactccataa cattgcaagt gctttttgac tgcttttccc aatgcgttat
     9481 caggtacttt tgtagcaaaa atcgcttcat gtccgtaatt agctaaagaa attgctacgt
     9541 ttgcttcccc tccgccataa tgggcagcga atcgttcact tgaatcgata cgggttccct
     9601 gctctgtgga caatcgcaac atgatttctc cgagtgtcac tacttttccc ataagcagct
     9661 acctcttgat ttctttcatt tttgctgcat actgttgcgc gacttccgtt actttgtcaa
     9721 aatctcctgt agctgcgggt gctaaaaggt tcccacctac tccgactgtt acgacaccag
     9781 catcgaacca ctctttcata ttgtccagac tcacaccgcc agttggcatg atattgactt
     9841 gaggcatcgg cgccttgaag gcagaaatga tacttggtcc ataagcgctt ccaggaaata
     9901 gtttgacgat atctaccccg cttttcagcg ctgttttgat ctcggtaata gtcatacatc
     9961 ctggtaagta aggaatttga tacagattgc acatctctgc cgtttcttga tcaaaggaag
    10021 ggctaacaat atactctgcg ccagctaaga tagccaagcg agctgtgaca gcgtctaata
    10081 ctgtgcctgc gccaatcact atttctggtt ggtctttgta gaatgacaaa agttctttga
    10141 ttacttgatc tgcttgaggg actgtaaaag tcaactcaat accagttaga ccacctttga
    10201 tgattgcgtg gcaagcattc aaagcttctt ctttgctttt tccgcgtacc accgcaatca
    10261 ctccggcgtt tttcaatcga gataaaatat caattttttt catcttctcc acctatttcc
    10321 tatttagaaa ttttatttta taacttggaa atctataccc atattatacg gcagaaaaga
    10381 aaaattgcaa acgctttttt cattattttt gaattctttg ctcgatttcg ctttttgttt
    10441 gcttgagggc actgattatt tgttcttttg tctcatctgt taaacgatat ttcggcatac
    10501 ttacactaaa tgcaccaatg atttgttcgt ttttcacgat tgaggctcct atacaaaaaa
    10561 tatcacgttc gatttcttca tcgtcaaatg cgatgtgggt ttcttttacc gtttggattt
    10621 cctttttcaa ccgtaatgca ttggtgatcg tatattctgt gtaagggatc aacggatgct
    10681 tttcaagata atcagtgtac tgctcttcac tgaattctgc taatacggct ttgcccatcg
    10741 cagaattgta taatggtcgg gtgattccga tttttgaaga catgcggatc gtttgatgtt
    10801 ttggatctag tttgttcacg tacaagatct cattgttgtc caatacccct aagtggatag
    10861 tctcatcgat cttttgctgc agctgttcta aaaacggtaa agtcaactct accaaatcga
    10921 tttgttcaat gtttttattc gcgtagcgaa taagctttgc acctaaacga tagtttttct
    10981 ctgaattttt atttacgtag ccgatcatca acaaagtatc tagtattttc aagactgtag
    11041 aagaggtcat tcccacaccc tttgcgatcg tctgcaaaga gacatcttgg ttttccgcta
    11101 aatagtctag tattttagag gctttgatca atacagttcc ataaggcttt ttattttcca
    11161 tataatattc tcctatttct ttataagaaa aattatagcg gaaataccat caatttaaaa
    11221 gagatatcca gatataaact accatttctc tttacaggca atttaatgtt gaagatccgc
    11281 ttcgttttta catcaaactt tgatataaag caatgatttc ttctactgtc gtttctcttg
    11341 gatttcctgg tgtgcataca tctcgcaaag catcttctgc aattgctgga atatctgctt
    11401 cttttacacc taattcagaa atggtttctg ggattccaac cgctttgctc aaccgatcga
    11461 tctcaccaca agccgcttct cttacatctt ctaaagacat ttcagctgct cctttaatcc
    11521 ctagaacaag agcgatctca cggtactttt ctccagtata ttctttgtta tatttcatga
    11581 cagttggcaa aagtgctgca cacgctacgc catgaggaat gttgtaccat gcacttaacg
    11641 ggtgagccat cccgtgtacc agtcctaaac cgacatttga gaagcccatt cccgcaatat
    11701 actgtcctaa tgccattttt tctcgcccat tttgatcgcc atctacagaa gcttccaaag
    11761 aatggccgat gatttcgatt gcttttaaat gcagcatatc agtcatttcc caagcgcctt
    11821 ttgtgataaa cccttcaata gcatgcgtca tagcatccat acctgttgat gcagccaatt
    11881 ttttcggcat tcccatcatc atatcgctgt cgatgaacgc gacgattggg atatcatgag
    11941 gatcaacaca aacaaatttt cggtgattta cttcatctgt gatgacgtag ttgatcgtta
    12001 cttctgctgc tgttccagaa gtcgttggta tagctaatat aggtaaacat ggatttttcg
    12061 tatcagcaac accttctaaa ctaatgacgt ctgaaaactc tggattcgtc aaaataatcc
    12121 caatggcttt tgccgtatcg attggcgaac ctccaccgat tgcgatcaaa cagtctgcct
    12181 ctgcctcttt aacaaaagcc actccatctt ttacattttg gatcgttgga ttaggtacga
    12241 tcccgtcgta taaagcataa gcgattgctg ctttatctag aagttctgtt acttttgttg
    12301 ctataccgtg ctcgatcaat cctttgtcag taatgacagc tgctttgccg aatcctcgct
    12361 gctgaaattc agggacgatc gactcaatcg ctccttttcc aaaatacgat gtttcattta
    12421 aaatcattcg attactcatt ttctattcct cctaatttgt attatttttc acaataaaaa
    12481 cgaaagaacc ccaagcaacc ggttgcccag ttgcttgttg ctacagttca aagactggca
    12541 tcaattccca actgactgga gaggcatctg gatttgtctc catgatatct tccatgtact
    12601 tccaccattt ttgattgata gatgtatttg ccattttttc ccataacttt tcatcttcga
    12661 tcactaagta accaaataac aaacttgtac tcggatcaag atagatcgaa tagtcgatga
    12721 ccccgtgttc acgaagtgct gtacgcattt ctggccaaag ttcatcatgt ctttttttgt
    12781 attcttcttg tttttctggg taaactttca tgcaaaaagc ttttttcacc atgtcagaca
    12841 cctgctttcg tatgaacttc ctccaaataa cctgcttttg gagtgacacc aaacgcatct
    12901 gctaaatgcc acaggttttc gtccgtgatt gtttgcaaga ttggtccttg cgctttgacg
    12961 tacgtataga cttctgctgc cttttctgct gtctcgatca aaccaaagac ttcatccatg
    13021 tcttttcctg acccgtagat tccatgttgt ggccataaaa ccaatcgagt ttctttcatt
    13081 ttttcagctg ttgcttcccc aatctcattt gttcccggaa ccatccacgg aatgatggaa
    13141 attccttctg gaaaaacaac taagcattcc gtacacattt gccatagcgt acgactaaag
    13201 gaacgttcgt ctaattcatg cgtaaaactc atcgctaata gatgactagc atggcaatgc
    13261 ataacgatcc gattatctgg gtctacttct aaacgggcga tatgactcat gaaatgactt
    13321 ggcaactcgc ttgtaggaag gcattcgttt tccagccccc acaaaagttc cagtgttttg
    13381 ccatcttcag caactcgaac gattccaaga ttctcggcag gttcgctcgc tacattttta
    13441 aaatatcttc ctgaaccagt tacaataaaa tagcatcctg ctaaacggct ggcatcaaaa
    13501 atcatcgaga tgcggcggat cactttttca ggatccaaga acggtgtgat ctcttcttct
    13561 tttagtaaat aagaaatatt cccgccattt ctttcatccc atcccaagcg ataaagattc
    13621 gacgtcgttt cgatcatttc tttcacataa ggcgcttgta aaatatctat ttttttcaat
    13681 atagccacac tcctattttc ttaattccaa cacgtctttt tgatattcct ggacttcttc
    13741 cagccaatcc aaaccaactg gaacttgatt tttgtaacag aaataattcc atacatcggc
    13801 atacgggaaa tctttcaatt cttccgtata agccattctt gaagtaaagt caaaagccag
    13861 ctccatttct ttcaatcgct caactggttc cagcatagct ttcagcagtg ctttttgtgt
    13921 atttctcgta ccgatgaccc aagcagcgat acggttgatc gttgcatcaa agaaatccag
    13981 accgatattt gttttttcca acaattcgtt acggactaat tctttagcga tttcctgcaa
    14041 ttcatcatcc atcaaaacca catggtctga atcccaacgc actggacggg aaacatgcaa
    14101 catgattcct ttgctgaata aagctaatga agataattta ttggaaatca cttctgttgg
    14161 atgaaaatgt ccagcatcta gacaaacaag tttgtcacga gtcaacccgt aacccatata
    14221 aaattcatgg gaaccgactg tataagcttc agcgcctaaa ccaaatagtt tgctttctac
    14281 tgcatcttgt acataccgtt catctaactc ttctgtaaaa atttcatcga gtgattccat
    14341 taaacgttcc cgtggggtaa aacgatcaat cggattatct ttcatcccat caggtaccca
    14401 aaagttattg atacttgttt gcccagtttc tttcccaaag tactccgcga ttttgcggct
    14461 tcttttcccg tgttcgatcc agtattcacg aacttcttta tctggatgag ccaatgtaaa
    14521 gccgtctttc atcattggat gcgaaaagaa agtcggattg aagtcaagac cgatttcttc
    14581 tttttttgcc cactctaccc atttttcaaa atgtttcggc tcaatttcat ttagatcgac
    14641 agattcttct gtatccaaat agatagcatg aaggttcaat ttgtgtttgc ccggtatcaa
    14701 cgaataagcc ttctccagat cttgtctaag ctcttcaggt gttcgtgcgg ctcctggaaa
    14761 attccctgtc gccatgattc caccggtcaa ttcgccatcc ggtgtcaaga aacctttgac
    14821 atcatctcct tgccagcaat gcatagaaat tttgatgtcc tgtatctttt ctagtacagc
    14881 atctgtatct acaccgatag ttgcatattt ttcttttgct tgttcgtaat ttctttcaat
    14941 cgtgttcata cgattactcc tttgatttta ttcacgtacc attccatcgt gttttgctta
    15001 ttttcatctg ggtaaaaagt ttcacatgga aacgactgtt tgattacatt tcttgcttgt
    15061 tctatattcg aaaactgacc tgtactgagc atttgcatgg caacattccc gattgctgtg
    15121 gcttcactag gtcctgcttc tacaatgatt ccggcataat ctgctgtcaa ttgattcagt
    15181 agacgattat tcgatccgcc ccctacgatg tgcagctttt ccaatttcgt tccagttaag
    15241 tgttctactt tgatcagctc ttgtgcgtaa caaagagcaa gattatcata cacacaacga
    15301 gcgatagctc ccgatgagac agggactggc tgctctgttt caaaacagta ttgctgaatc
    15361 tcttctatca tatttggggg attcaaaaat cgagggtcat tgatttccat cacttgctgc
    15421 agcggttttt cttttgcagc aagctctgcc aattccgcat aactgtactc gtactcttgc
    15481 attcgggcaa cttcctggat caaccacatc cccatgatgt ttttcaaaaa acgcaccgtg
    15541 ttatgggcac cccattcatt cgtataattt tcagaaaaag cattgcgaga aacattggcg
    15601 atctttgttt caatccctaa aagcgaccat gtcccactgc tgatataacc ccaacgttct
    15661 ccttgacctg gcgttcctaa tacagctgac gccgtatcat gacttgctac tgtgataaag
    15721 gtcacatctg gcaaatcata ttttgaaaat tgttcttttt gcagatctcc tagtatcgtt
    15781 cccggctctg tcaatggtgg aaataattcc ttcgagacac cgattttttc aagaagttct
    15841 atttcccatt cttttgtccc tgcattcagc agctgcatag tagatgcatt agttttctcc
    15901 gtcaccatgt taccagtaaa gacataccct aaataatcag gtatcagtaa caatttatgt
    15961 gcttttgcca aattttcttt ttcttcagca aacaattgga acaaggtatt gaaaggctgg
    16021 atctgaatcc ctgtctgctc atacaactta tctagagaat agatttcaga aaactgttct
    16081 actgctccat tggtacgctt gtcccgataa gcaatcggat catttatccg atccccttgt
    16141 tcatcaatca gacagtaatc gactgcccat gtatcgatcc ccgcaaaaag gcgatcaatc
    16201 cctgtctgct ttaatttttc aaatccaatc aaaatctctt tgataagata atcgatcttc
    16261 cagcgatcgt gaccgtcttt gttccgaaag ccgtttttga accggtgtac ttcttccaag
    16321 atcaaagaac cttttgcatc cctcgtactt ttcattagtc gtccgcttga tgcaccgata
    16381 tcgatggcaa caaatgttgg attcatttct ccacctcttt tactttattg ttgattagca
    16441 ctttattaat aaagcgttat catttattaa caatgctatc ataatacaga atcttatatt
    16501 ttgcttcaca ctattttggt atataattgc attatcttga caagttggga gtgtttacat
    16561 ggattactgg gaaaaagcca accactcatg gtcggaagac tcaaaaagag tgatcttgac
    16621 gcctacacaa agaagcagga atttattttt ttatatccaa gaaatcggtt acttcaaagc
    16681 accaatccct tactatactg aacgagcaca tttaccttct tacttgataa agtttacttt
    16741 gtctggcaaa ggttcgctta catataatga taatcagcaa cagctaaaag ctggagactt
    16801 attttttatt gactgccaag aatatcagcg ttatcaaacg atcagcgaag aaccatggga
    16861 aatggattgg atccatttga atggtcagaa cgtccaatca ttttatcagg agttcacaaa
    16921 agacggccat aacaccttcc attcaacagg ggatccatca gaaaacaaga tccaccattt
    16981 gatcacagaa ttgatccaca tgcaacaagg cacgaatgcc cgtaccgatt tccagtcctc
    17041 tgtagctatc cacgagttgc tgaatgaact gattttgcaa aagtatcagc tagatttcga
    17101 agaagaggat attcccgcct acgtttacaa actgaagcaa tacttagacg aacatttcaa
    17161 agaaacgatt acacttgatc aattagaaca acaattctta ttgaataaat atcagctgag
    17221 caaagatttt tccaaattta tcggttctcc accaatcgat tatttgatca gcaaaaaact
    17281 ttcttacgct aaagaccttc ttcggtatac agaatttacg gttcgaatga tttccgaaga
    17341 aatagggatt gagaattttg cttactttag ccgcctgttc aaagcaaaaa caggactatc
    17401 gccgagagac tttcgtaaat tcagctgatt catataatat gtacgtcatt ttttcaaaat
    17461 attttgaaat atatagataa aaataaaata accgcagttt ctgcttctat gtaaacaaca
    17521 gcagctactg cggttatttt ttagtgatca ttctcattgt tcatcttcaa aatgttcata
    17581 atgaaccaac tgtttctgtg tatcacgttt caaaaattct ttaggcgtta tgccgaactc
    17641 tctcttgaat gctttcagaa aatgcggata agaagaaaaa ccacttttac tatatacagc
    17701 actagagctg cggtattctc gcaacaagtg cttggcatag atcagctttt tcttcattac
    17761 atactgatga aacgtaaatc cagtgtactt tttaaattct cgtgtgatat ggtatttgct
    17821 gacaaaaaac tgcttttcca tttcctctaa agaaagcgga tgagataaat tttttgagat
    17881 atattgtaac atttgttcca cacgctcatt aggaactaca gcattctgtt cttgcgcact
    17941 ctcttctttt tgtacaactt gattgagaaa aatcaagtat tctaccagag cttgttcata
    18001 ttttatgtct tcaccgtaaa tttttttatc aggaggatgg tcgataaatg ataatttttg
    18061 cttcaatact tcctgttcta tttgcaatac acgactcctc ttctctccca ttggttgaaa
    18121 gcaagccgat agattagacc atggcgtgga acgactttct agaaaagaag gggtgacaaa
    18181 gatatagaca cgctcgaata catctgaaga ttggcggacg atacgatgca aatcattgga
    18241 atggatgagc agcacacttc ctgctttgat cgtaaattgt cgtccgttca aataaaaagt
    18301 cgcttctccc tttaatgtag catggatctc atagaaatca tgatgatgat agatcgtttt
    18361 attttggctg acgacatctt tgacatgata gatttcatac tgatcagaaa ccatcgtatt
    18421 gacttgttca ccgctgattt tcttttggag catgtgccgc acctcctttt gttcacttat
    18481 gcacaatttt attataccac gttttttgta atcaaaaaga gctgctttta tatactctta
    18541 taaaaaagaa gagacgtggc atcttctagc cccgccccct ccattctaaa tattagttaa
    18601 ccaacgtctt ctttacatta ttcaagaaga cttcttccat aacgaatccc atcatctttt
    18661 gtttcttcaa gaaccacata caggtacgga cgattcttca tgatgatttc tttgattttt
    18721 gtgtactcga tcacaccgtc tcccaaattt accggcacga ttttttcctg ttcgacacga
    18781 aaatctttca aatgaaccgc acatatcttt tcaccaaaac gttcaaaagc ttcttctacc
    18841 aattggactt gttcttgata atcactactt gtgatcaaat tggtcggatc taaaatgatt
    18901 cctagatagt ccgaatctac tgcttggatc agctgctcta cccttgctaa agaatacacc
    18961 ggatgattca aacctggttc tatccccacc atcatttggt atttttctcc agcttttacc
    19021 aagcgacgga taacggacac agcttcagca aatgcttcat ctgtgaaatt ttcttccgta
    19081 tattggattt ccggcaaaac acatccagtt tccgaagcaa ccatcgacgc accaaaggaa
    19141 gaagcatagc gaagatactg ctcgaacttt gtcagcaact cttgtcgtat cgttaaatct
    19201 ggatgaatca tattgatata gcaacttaaa attcctacgg tcacttcttg cttttctaaa
    19261 actcgtttga cataatttcc catcccagaa ttgatctcat ttgcgccaga aggtatttca
    19321 ggaaacgaaa gacctaaggc gagctgcagt gtatgaatcc cttgttcttt tgttttttct
    19381 gcaagatctt ctatcgtttg gacttcggaa agatcatgtc ctctaattgt aatgttcaac
    19441 ataactattc tcctaactca gcatattggg tagccacatg acgatttgcg ggaagtaagt
    19501 aatcaaaaac aagacgacta agataacccc ataaaaagga agaagcggtt tcagcattgc
    19561 ctctgctttt acgcctccta cacttgcacc gacatatagt cctgtaccta ctggcggcgt
    19621 gatggagcct acacacagat tcatgattga aaacaacccg taatgtacag gatcaacccc
    19681 tacacttgtg acgattggta aaaatattgg tgtaaaaatc aagatagcag gcgccacatc
    19741 cataaacata cccaccagta ataacacaag attgactaat aacagaataa taaatttatt
    19801 atccgttacg cccataatca acgaactaat tgcctgagga ataccggtaa acgccatggc
    19861 gaaagacatc atggaagaag tagccaataa gaacatgatc gtccctgaca ttgctactga
    19921 ttgaaacaac atattaggta tatcttttag ttttaccgtt ttatagtaaa acatagcaat
    19981 caacagggag taaatgacag ctatcgctga agcttctact gctgtaaata tccccgtcaa
    20041 gataccaccg ataatgatga caatcaatag aaggctagga atcgcctctt ttattatttt
    20101 tccagcatga gccatctgtc ctttttcgac aacagggtat ttattctttt tagcaacgat
    20161 gaatgctact agcatgattc ctagcgccca caaagcgcct acgagataac ctcccataaa
    20221 caaagcagag atcgaagcac caccagcaac aagtgaatac atgataaaag cagtacttgg
    20281 aggaatgacc atacctgtcg gagctgacgc gatattgacc gctgtcgcaa acttccggtc
    20341 atatccttct tccacttgct gagggatcaa cactccgcca atcgcggtag aagcggcaat
    20401 ggccgagcta gagatggaac caaataaagc attccctaac acattcgtat gagccaaagc
    20461 ccccggcacg cgcccgacga acaacatcgc aaaatccact agtttcttag ctatccctcc
    20521 attattcatg ataatgcccg agagaacgaa aaacggaata gcgatcagcg aaaaactatt
    20581 gatacttccc accatttttt gagctgaact aaaagcagaa acatcaaacg gtataactaa
    20641 caataaagtc atcatcgatg agaatgcaat acttatagca atcggcatcc ctaatacaag
    20701 caacacaaca aatacaaaaa ataacactaa tccggcttct attgccattt tattcctcca
    20761 tttttatatt ttttcttgaa ttgctggctt gttgcttaat gtatcgatta gattgatcag
    20821 gctatagata atgatgagta cccccgatac aggtactgac agataaacat atcccatagg
    20881 aatacttaaa gaaggcgaga tctgccccat tgtcaatgtg acagcattcc atccgccata
    20941 caccatgatc atcgcagcaa ataccagaaa taaactttga acaacaactt ctaaaatttt
    21001 actttttttg ccagttaatt tttcacttaa aaccgttacg gctaaatgtg ctttttgtcc
    21061 cacaacatac gcggaagaca acatggcaaa ccagacaaga ccgaatcgaa caagctcttc
    21121 cgtgaccgta tttggatttc ctaaaaccgt tctggaaaat acttgccata aaacaacagc
    21181 gaccatccca acaagcaaaa cagctcccag gatctctaat accttgttta aaatcatttt
    21241 catcacagct acgctccttg ctcttggatc ttttgataaa ggtctttgta ggtaggatcg
    21301 tttttgaata tatcatgaag tggggcaact gcctctcgaa acggtgtatt atctacatca
    21361 ttgaattgga ctccatgttc ttcttgtgct aaagcttttt cttcttcaat tgcttttgca
    21421 aaagcttcaa tttcaaaaac cgttgattct ttcgctgctt cttcaatggc tttttcctgc
    21481 tcatcagtta gctgttcttt gatggcatca ttcatgatga tgatatctgg cacacgtgta
    21541 tgctcatcat aggaatagta tttagccaca ccaccatgtc cagctgtata caagacaaat
    21601 tcattgtttt ctgatccgtt gatcaaattg gattgaagag acgtgtacac ttcatcactt
    21661 cccataggta cgggcgaacc gcctaaaagc tctaccattt tgatagcggt ttcgctttgc
    21721 atcacacgga tcttctttcc cttcaaatct tccggcgtat ggatcggacc gtctaccata
    21781 taaaagctgc gctgaccaga atcataatag gtcaatccgg taaatcctaa atttttggtc
    21841 gattggtaga ttggctccat tatttcttca ttatccatca ccttaaagaa atgttcttca
    21901 tcattgaata gataaggaac tgaaaaaatc gaataatctt tagagaaact ttccaacgct
    21961 gaggcactta cttttgtgaa atctactgct cctgactgtg tcaattcgat cagttcacgt
    22021 tctccaccca gttgtccatc tgggaaatac tggatctgta cggaaccatc tgttttttct
    22081 tttactaaac gcccaaattc attcattgca tcaattactg gctggctgtt gcttgcataa
    22141 gcaaagcgca aggtaactat ctcatttaca tctgcatctg cgacagttgc cacactagaa
    22201 ttatttccgc agccgcttag acctagcaga cctaggctaa gcgaacttgc tgcaaacagc
    22261 agtgatgttt ttttcatatg ggtttccttt cttttaacaa taagagacga gcaattctgt
    22321 caaacaaagc accgttaacg actgtccata aggcattgct gtgattccga tattccgata
    22381 aaaatctaaa ctgtccccca ttcctgtccc gattgaaacg ttttgaactt ctcctttttc
    22441 atcgatttgt tcaagcaatc cttggatagc acgataagcg acttctttat attcttgtgg
    22501 taaataacgt ttatggaccg ctttcaaaat accgtaagca aatcctgcag ttgcagaaga
    22561 ttctacgtaa gaagaaggat catccaacaa cgtatgccat aaaccgcttt cagactgata
    22621 agtttttaat gcacgcactt gtgcttcaag tgtttcgatc aataatttgc gcaatgcatc
    22681 ttctttcgat aattctaaga tttcaatgat ctctggaata gcaatcgtca accagcagtt
    22741 tccacgagcc caaaatgctt cggcatagtt atgatttcct tcaaacgtcc agccgtgata
    22801 ccacaatcct gttcgtttat ccatcaagta tttgatatgg atcataaact ggtaacgagc
    22861 ttcttctaaa tattctgggc gattcaacag tttcccgatt tttgccaaag gaagcactgt
    22921 catcatcaat gtatcgtccc acaactgatt cttattttcc ggtccatatg tggcatgctg
    22981 taacccgtct tcatttgtcc gaggcatgtc attcatgacc cattctgccc attgttctaa
    23041 ataaggcaaa tagcgtgaat ctttcgtgtc ttcatacaag taagccatag tcaatagcgg
    23101 tgccattgta ttgacgtttt tgggcggagc gccttcttcc atacgatcct caaaccattc
    23161 attgaccact tgataagcgc gaggattttt tgtcatctgg taatttttat aaataccata
    23221 tagaccgaca ccttgtggcc aattccacac tgtccagctt ttgtcatcta ctttcagtcc
    23281 atcaaagtcc aacaaaaatt caccattatc gtctttgatc gttgtcaggt tttcaacgag
    23341 caggtcaatt ttttctttca ctaattcttt aggtacaaaa atctcttctt taataatcat
    23401 ctttacgcca cttttttaaa cgctttcatt ttatatcctt aatatagcag taataccgtc
    23461 cactataaat atcgcttttt gaacaaaaac tgtctaaaat tgacctaaat gttaaataac
    23521 aaaagtaact ttcagttact tggaaattaa aaaaagaact tggagaaaca acgttt
//
LOCUS       DACCCM010000043        23571 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCCM010000043 DACCCM010000000
VERSION     DACCCM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.045"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK78_001655"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK78_001655"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP6375130.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IVK78_001656"
     CDS             complement(1172..1867)
                     /locus_tag="IVK78_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP6375131.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IVK78_001657"
     CDS             complement(1880..2596)
                     /locus_tag="IVK78_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP6375132.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IVK78_001658"
     CDS             2926..3531
                     /locus_tag="IVK78_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP6375133.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IVK78_001659"
     CDS             3856..4179
                     /locus_tag="IVK78_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6375134.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IVK78_001660"
     CDS             4361..5731
                     /locus_tag="IVK78_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP6375135.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IVK78_001661"
     CDS             complement(5861..7162)
                     /locus_tag="IVK78_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6375136.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IVK78_001662"
     CDS             complement(7392..9383)
                     /locus_tag="IVK78_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP6375137.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IVK78_001663"
     CDS             complement(9527..9793)
                     /locus_tag="IVK78_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6375138.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IVK78_001664"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IVK78_001664"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP6375139.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IVK78_001665"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IVK78_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP6375140.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IVK78_001666"
     CDS             13137..13700
                     /locus_tag="IVK78_001666"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6375141.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IVK78_001667"
     CDS             complement(13953..14474)
                     /locus_tag="IVK78_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP6375142.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IVK78_001668"
     CDS             complement(14477..15319)
                     /locus_tag="IVK78_001668"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP6375143.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IVK78_001669"
     CDS             complement(15312..16037)
                     /locus_tag="IVK78_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP6375144.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IVK78_001670"
     CDS             16213..16509
                     /locus_tag="IVK78_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375145.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IVK78_001671"
     CDS             17077..18669
                     /locus_tag="IVK78_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6375146.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IVK78_001672"
     CDS             18682..19422
                     /locus_tag="IVK78_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6375147.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IVK78_001673"
     CDS             19590..20939
                     /locus_tag="IVK78_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6375148.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IVK78_001674"
     CDS             complement(21067..21657)
                     /locus_tag="IVK78_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6375149.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IVK78_001675"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IVK78_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6375150.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DACCCM010000044        23368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCCM010000044 DACCCM010000000
VERSION     DACCCM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.046"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(114..185)
                     /locus_tag="IVK78_001676"
     tRNA            complement(114..185)
                     /locus_tag="IVK78_001676"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Arg,seq:ccg)
     gene            245..1579
                     /gene="rpoN"
                     /locus_tag="IVK78_001677"
     CDS             245..1579
                     /gene="rpoN"
                     /locus_tag="IVK78_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP6375151.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            1782..2819
                     /locus_tag="IVK78_001678"
     CDS             1782..2819
                     /locus_tag="IVK78_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP6375152.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            2860..3861
                     /gene="gap"
                     /locus_tag="IVK78_001679"
     CDS             2860..3861
                     /gene="gap"
                     /locus_tag="IVK78_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6375153.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            3971..5161
                     /locus_tag="IVK78_001680"
     CDS             3971..5161
                     /locus_tag="IVK78_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP6375154.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            5265..6020
                     /locus_tag="IVK78_001681"
     CDS             5265..6020
                     /locus_tag="IVK78_001681"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP6375155.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            6136..7434
                     /gene="eno"
                     /locus_tag="IVK78_001682"
     CDS             6136..7434
                     /gene="eno"
                     /locus_tag="IVK78_001682"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP6375156.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            7833..8972
                     /locus_tag="IVK78_001683"
     CDS             7833..8972
                     /locus_tag="IVK78_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP6375157.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(9039..9614)
                     /locus_tag="IVK78_001684"
     CDS             complement(9039..9614)
                     /locus_tag="IVK78_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6375158.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(9619..10593)
                     /locus_tag="IVK78_001685"
     CDS             complement(9619..10593)
                     /locus_tag="IVK78_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP6375159.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(10617..10661)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            10876..11844
                     /locus_tag="IVK78_001686"
     CDS             10876..11844
                     /locus_tag="IVK78_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6375160.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            11941..12234
                     /locus_tag="IVK78_001687"
     CDS             11941..12234
                     /locus_tag="IVK78_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6375161.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            12352..12588
                     /gene="secG"
                     /locus_tag="IVK78_001688"
     CDS             12352..12588
                     /gene="secG"
                     /locus_tag="IVK78_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP6375162.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            12700..13446
                     /locus_tag="IVK78_001689"
     CDS             12700..13446
                     /locus_tag="IVK78_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6375163.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            13449..15806
                     /gene="rnr"
                     /locus_tag="IVK78_001690"
     CDS             13449..15806
                     /gene="rnr"
                     /locus_tag="IVK78_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP6375164.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            15828..16292
                     /gene="smpB"
                     /locus_tag="IVK78_001691"
     CDS             15828..16292
                     /gene="smpB"
                     /locus_tag="IVK78_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP6375165.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            16594..17313
                     /gene="atpB"
                     /locus_tag="IVK78_001692"
     CDS             16594..17313
                     /gene="atpB"
                     /locus_tag="IVK78_001692"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP6375166.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            17366..17581
                     /gene="atpE"
                     /locus_tag="IVK78_001693"
     CDS             17366..17581
                     /gene="atpE"
                     /locus_tag="IVK78_001693"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP6375167.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            17672..18196
                     /gene="atpF"
                     /locus_tag="IVK78_001694"
     CDS             17672..18196
                     /gene="atpF"
                     /locus_tag="IVK78_001694"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP6375168.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            18183..18725
                     /locus_tag="IVK78_001695"
     CDS             18183..18725
                     /locus_tag="IVK78_001695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP6375169.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            18748..20304
                     /locus_tag="IVK78_001696"
     CDS             18748..20304
                     /locus_tag="IVK78_001696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP6375170.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            20318..21220
                     /locus_tag="IVK78_001697"
     CDS             20318..21220
                     /locus_tag="IVK78_001697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP6375171.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            21243..22649
                     /gene="atpD"
                     /locus_tag="IVK78_001698"
     CDS             21243..22649
                     /gene="atpD"
                     /locus_tag="IVK78_001698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP6375172.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            22663..23085
                     /locus_tag="IVK78_001699"
     CDS             22663..23085
                     /locus_tag="IVK78_001699"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP6375173.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         7867 a         4238 c         4990 g         6273 t
ORIGIN      
        1 caatatctat acagatacaa caaaaaactg tccaccaatt ctctttggcg gacagttttc
       61 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
      121 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
      181 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
      241 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
      301 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
      361 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
      421 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
      481 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
      541 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
      601 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
      661 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
      721 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
      781 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
      841 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
      901 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
      961 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa
     1021 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
     1081 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctccaacg
     1141 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
     1201 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
     1261 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag atttcggtgt
     1321 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
     1381 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
     1441 aaacaagccg ttgtcggacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
     1501 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
     1561 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc
     1621 tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc
     1681 ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta
     1741 agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa
     1801 tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac
     1861 gaaatatcta ttggatgcag ccgattgggc gtcgaagttt atcggaaagt atggggatca
     1921 cagaacgagt cttacgaaca gaaacagacc ttttgaaaaa tctgaatttg atcgacacct
     1981 caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact
     2041 tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta
     2101 accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat
     2161 tcggcgaagc gctgagcgaa gccttagacc gactacttcc agaaggcgag aatatcatcg
     2221 ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata
     2281 agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac
     2341 aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgcgcacttt
     2401 atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc
     2461 aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat
     2521 tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa
     2581 atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg
     2641 tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg
     2701 cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa
     2761 cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa
     2821 ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt
     2881 aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc
     2941 gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac
     3001 gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta
     3061 aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa
     3121 ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa
     3181 aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt
     3241 ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca
     3301 ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt
     3361 ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta
     3421 gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt
     3481 cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcccgaatt gaacggtaaa
     3541 ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca
     3601 gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac
     3661 gaatcttacg gatacaacac agacgaaatc gtttcctcag atatcgttgg tatgacttac
     3721 ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt
     3781 aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta
     3841 gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca
     3901 tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg
     3961 aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct
     4021 tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat
     4081 caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt
     4141 ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt
     4201 tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg
     4261 agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa
     4321 tacacgtttt gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa
     4381 atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc
     4441 acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat
     4501 ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgcc caatgattgc
     4561 aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa
     4621 agcagataaa atcttaatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat
     4681 caagatcggt aactctctag tagaagctga taaagtagaa ctagcaaaag aattaatcga
     4741 aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa
     4801 tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat
     4861 cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg
     4921 gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg
     4981 tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagctgc
     5041 agcagcagaa caattaggat tcgctgacaa attcacacac atctcaacag gtggtggcgc
     5101 aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata
     5161 aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat
     5221 gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta
     5281 tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag
     5341 taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat
     5401 tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact
     5461 gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc
     5521 taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag
     5581 acgaagacat caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca ccaatcctat
     5641 gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac
     5701 aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt
     5761 atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa
     5821 tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag
     5881 ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag
     5941 aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca ttcttgtcat
     6001 tgttagacgc tgtaaaataa ttgttgaaaa tttgtgaaaa atgcaagaaa tgtttgcact
     6061 aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca
     6121 aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca
     6181 cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga
     6241 atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac
     6301 aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt
     6361 gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc
     6421 gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct
     6481 atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc
     6541 ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat
     6601 gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc
     6661 aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct
     6721 cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac
     6781 gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa
     6841 gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt
     6901 tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac
     6961 gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga cgaaaacgac
     7021 tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac
     7081 gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac
     7141 tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa
     7201 atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat
     7261 tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac tggttctcta
     7321 tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt
     7381 gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt
     7441 tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt
     7501 aatcgaactc tctacttgct gaaatatcag caggtagaga gtttttttgc aactagcttc
     7561 tgtatatgat gtttacaaag aatggtacaa gaaaagatca tccttgaaaa aggaaagcta
     7621 acaataacta gcatcatata agagctttta aggatagaac agtagaatag gaatctatga
     7681 gcaagtcctc tcgtataaat aaaataggag ggaagtagca gcatattttt cataaagtgg
     7741 ctctctttct tcatttttac tttggttgct cctatttgaa aattcgagca cactcaaata
     7801 caataaaaag aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa atcgcgattt
     7861 tacaagagat catccgaatt aaatcagtga atggaaacga aggtgaagta gcagcctatt
     7921 tgaataagct tttggcaaaa catgacataa caggagagat tgtttcttac agagatggta
     7981 gagataattt gattgctcgt taccaaaagg gccaatcagg aaaagtgtta ggcctatctg
     8041 ggcatatgga tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca ccctttgcag
     8101 cagaaatcca tgggaaccgt ttgtacggac gtggtgctac agatatgaag agtggcttgg
     8161 cagcaatggt gattgccatg atcgaactga aagagtcagg aaaaccattt aacggaacag
     8221 tcaaattatt agccactgtc ggagaagaag tgggtgaact aggtggggaa cagctcacga
     8281 aagcaggcta tgtagatgac ttagatgcct tgatcatcgg agagccaacc aattacagtt
     8341 tgatgtatac gcacatggga tcaatcaact atacagtcac ctcgcacgga aaagaagcgc
     8401 atagctctat gccagaccaa ggttataatg caatcaatca cctaaatgaa ttcattacga
     8461 aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga gaatcctgtc ctaggaaaaa
     8521 caatccataa tgttacattg atcagtggcg ggaatcaagt caacagcatc ccaagccatg
     8581 cgcagctcca agggaacatt cgatcgattc cagaataccc aaatgataaa atcattgcat
     8641 tgctgcaatc aatcgtcaat gaattgaatc aagagacaga ctatcatttg gaattgatga
     8701 tcgattacaa caaaatacct gtcaaagctg atccagattc tcctttgatt cattgcattc
     8761 agcaacaatt cagccagcca ttacctttag ttggagcggc agcaacaacg gatgcagccg
     8821 aatttacgaa agctaaccat tccttcgact ttgtcgtctt tgggcctggt gtcgttactt
     8881 tgcctcatca agtagatgag tatgttgaaa tcgataatta tttggatatg atcgaaaaat
     8941 atcaaggaat tattttatcc tacttagcgt aagatttttt taataaatga aaaaacagag
     9001 ccagattagc attattttgc taatccagct ctgtttattc agcctatttt tctttttgaa
     9061 tatatcaagc gttgtggcaa catagtgatg actaatgaaa caacgacgat agacaacaaa
     9121 cgatcaaggt agtctgtacc tgcctgtacc aagatcacac ttgttgtctg acttaaacct
     9181 aacccatgta atagctgaac gatcatactg gatcctgagg aggtgatccc gccaaataac
     9241 tgaacggtaa tgatcgaaga aacgatcgtt ccaggtaccg acaaaatcaa agcgagtaat
     9301 aacagtttca agctgttttt atctttgccg agatatcttg gcaaaatcaa tccagcaagc
     9361 aagcctgtga gaatttggat tggcgaataa tacagagaaa aaatatctgt agtcactcca
     9421 cttaaaatac cgctggacaa tcctgtcagc attccaggta caggcccaag caatgcacca
     9481 gcaaaaatcg ttccgataga atcgagatag attggcaaac gaagccataa agcaatcgtt
     9541 cctccaatat aattgatccc aatacacaaa gccatcactg taagtacgcg agttgccatt
     9601 ttgtttttat tcattttgtt agcccatctt taacctttcc aaatctttca taatcgtttc
     9661 ttcttgtgca tttaaaagaa cggttaaaaa ctttcggaaa aaaagacgag tatctacttt
     9721 tgtcataatt ttactgttag caggtttctg ccaaaattca taacgatcaa ccaatgtttg
     9781 tccacgactt attccttgag tttccacagc agtgaagctt ttgaaacctt cacagatggc
     9841 ctcctcgata aaataagcaa cagctaacgg atcattgatg acacatccta aaattctttc
     9901 ttgtttccag tggaagtcaa aataaaaacg agtgatcgct tttagatact ctccctcttc
     9961 aggattcatt tgacagcagt attcgaggat agtcggtgtc aagacgattt ctcttgtcac
    10021 atctaaccca accatttcta tcgtttgttt gagattctcg aatacataag aagctgcttc
    10081 aggatcacac cagtaattgt attcagcaac tggcgaacag ttaccatggc ttttgtaagt
    10141 tccacccatc gagacaaaac gttccatatg tttcccaata tttgggttga ctttcaaagc
    10201 agaagcgata ttggtcaaag gacccaaagc aatgactgat gtatttgtct tttctttgaa
    10261 ataatcagcc aaaaaatcaa cggcatggag aggttctgcc tgttttctca agatcatcgg
    10321 gaaatttgtt tcccctaaac catccatacc atgagtgtct tgtgcactga tgaaaggttg
    10381 cttcaaaggt ttttccattc cttgataaac aggaatgtct aaacgatcac agcgttccag
    10441 gcatttcagc acattctgcg tacctatatc tgcagggaca ttcccacaaa ctacggtaat
    10501 agcaactact tcgatatcag gggatttgac agctaatgat aaagctaaag tgtcgtcgat
    10561 ccctggatcg caatcaataa ttacttttcg cattatttcc aatctccttt ctttagagat
    10621 acttagtttt ttattctggg aagtttccga accagtccaa gacgagcgtc ttgattataa
    10681 tgcacgaaat tagtatactg gcttttattg cgaatgtaaa tgctctctta caagtaaatc
    10741 tattgaggag aactttaagt aaatgataca atgaaaaaga aatggaattt acgtgtgccg
    10801 aaaataaaga atcctatgga tcggtacatt ggataataga tagattatag tttggcagaa
    10861 agcggcgggt gtacaatggt tattttttat ttcattctta ttctgataat tatagggatc
    10921 agttaccatt ttctttctta tttttatgca aagaaattag taagagcggg agtacagaga
    10981 ggatatgact ggtatgaaga cactggtcat cgtttgatgg accctgctgc agtcacgtca
    11041 gtagaaaaaa aacgacaaaa attagaagaa gagtgtgaaa gcttttggga gcagggaaca
    11101 cccaaaacaa tcaaaagtta tgacgggttg aaactagctg gtcagatgtt catccatcca
    11161 aataagcaga ataaatgggt catttgcgta catgattacc gaagcacagg gaaaagagac
    11221 atgtctcata taggtaaaag atatgcagag aaaggattca acgttcttgt acctgattta
    11281 cgtgcgcacg gagaaagtga aggagaaatc atcggaatgg gatggctaga tcggctagat
    11341 ttaattgcct ggatccaact catcttagat gaacagccga atgcgtccat tattttacac
    11401 ggcggatcaa tgggagcttc tacgatcatg atggcaagtg gtgaaaaact tccatcagcg
    11461 gtcaaaggat ttattttaga cagcggatat gtttctgttt atgcagaatt ccgatatatg
    11521 ttaagtaaaa tcactgtctt tcctaaaaaa atggtcatga gatatgcgaa tcattatgcc
    11581 cagaaatacg ccggttactc cttaaagcaa gcatcagcta cgagacagct tggcagcaat
    11641 catctgcctt tattagtcat ccatggtgaa agagatcatt ttgtccctac agaagcagcg
    11701 tacacgattc aaaatgctac ggcaggggat aaagcactgc tgcttgttcc ggaagcagaa
    11761 cacttggaag catcgatgaa agatccgaaa acctattgga cagtgatttt ttcatttatt
    11821 gaacaaagaa taacgatgaa ttaattttct cttctttttt gtaagtaatt cgaggataaa
    11881 taattgttta tagatcagga gactgctaaa ataaggtagg aataaaaaaa tatcagttat
    11941 ttgaggagtg cggcatatgg aaatcaaaga agaaaaaatc gtcttgcttt attcaatgat
    12001 gaacaacaag agatcggaga aatgacatgg tcagatgcgg gcccagacat catgatcatt
    12061 gaccatacat ttgtagatcc agcatatcgt ggtcaaaagt tagctgaaaa gctagtgtat
    12121 actggagtag aattagcaag acgagacggg aaaaagatca ttcctttatg cccttatgca
    12181 aaaaaagagt ttgaaaagaa accagaatat caagacgtct tacgtcagtc ataagaataa
    12241 cagctgaaag ttttgaactt tcagctgtta ttccatttat atagagttgt cacggacttt
    12301 tatctatgat aaagttaaag agacgatatc ttttgggaag gaggctgcga tatgtataat
    12361 ttaattttgg gaatcgtgat cgttttatcg attgttatgg tcattgcgat tatgatgcag
    12421 ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg gtgcggatca actattcggc
    12481 aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat caactgcagt tatgggagct
    12541 atctggatga tcttattatt tgtcctagca tatttatctt cgaagtagtt gaataagcct
    12601 ctcgcaaaac ggcgggcggc tttttttatt gcaaagagaa cgcttaagaa gaagttatga
    12661 aagaaactat ggtaaaatga ggcgaaagag gtgagacgag tgagacaatt accaaaaccc
    12721 ctttatgcaa aacatggaaa gcgagcggtt ttgctgcttc acgcttactc aggaagtcca
    12781 aacgatgttc gaatgctcgc acgatttttg gaaaaagcag attatacagt ttatgcgccg
    12841 ctttttaaag gacacggaac aatggagcca tatgatatct tgcaagaaaa agcagaaagc
    12901 tggtgggcag acactaaaaa agccatccat tttttacagt ctgagcagtt ttctgatatt
    12961 gccgtattag gattatcaat gggcggcatc tttgctgtac gagcactaga agaagaatca
    13021 gtgattggcg gcggtttttt ctgttcgcct ttgtctcctg taaaaacaaa cgtgcctgaa
    13081 aactttgaaa aatacgtacg tcaagtgctg aagacagcag gtaaatcaga gaaagaaatc
    13141 aatgaaaaag cagttgctta ccgctcgtta gctgaacaac aattaatgga tatacaagat
    13201 caggcagcaa tcgtagaaag taggttgtca gatatccaac agccgatatt tttagctcaa
    13261 gctgggaaag atgaaatgat cgaccctaat ggcgtatttg aaactgcacg caagttgagc
    13321 cgtcagcgag tgacacttca atggtatcct gaaagtggtc atgtgattac agttggtaca
    13381 gcaagacgcg aattagaaaa agacgtcttg gaatttttag aaaaattacc ttggaatgag
    13441 gaataaatat gacaaaagaa acgataaaag agaaaattct ctttttcatg gaaagcagtc
    13501 gcaaacgcag tttttctatg gaagaaatcg ctgaaggttt agggttccaa aaaagcgatg
    13561 attttaaatt actagtacaa acgattgctc aaatggaaag agaacagagt atagttttca
    13621 ataaaaaagg aaaagtgaaa cttcctttaa aaccaatctt gatcgaagga acatttcgag
    13681 caaatgaacg aggatttgga tttgtaacga ttgatcccga agaagatgac atctatatcc
    13741 caaaagaagc gactggtttt gcaatggatg gagacaccgt agcaatcgat atcatgaagc
    13801 gagcaaatac agatatggat cgcggagcag aggggaaagt agtcgagatt cgagaccggg
    13861 cgactactca gttagcaggg gaattcgttg cttattcaga agaagaaata caagaaacag
    13921 atctttacgg cgttgttatc ccgaaagata gaaagctcaa ccaatttaaa gtatatgcag
    13981 cagcagaggg agttcgtcca gttgacggaa gtattgtgct gatcgaactg acccattacc
    14041 ctgaaaaagg gtatgcgact agtttagaag ggataatcaa gcaagtgatt ggtcataaga
    14101 atgaccctgg catggatatc ctctcaattg tcgtggctaa tggtatccct acgaaatttc
    14161 ctgatgatgt attgtctgaa gcagatgaag ttccagatca aatcagcgaa aaggatataa
    14221 ctggacgtag agatttaaga aatcagctca ttgttacgat tgatggagaa gatgcaaaag
    14281 atctagatga tgcagttacg gttaaaaaat tagataacgg taattatttt cttggagtac
    14341 acattgcaga tgtttcttac tatgtgacac aaggcagtca attggacaga gaagcatatg
    14401 aaagagggac aagtgtatat ttgactgatc gagtaattcc gatgatcccg caacgtttat
    14461 cgaatggtat ctgttcactg aatccccatg ttccaaggct gaccatgagt tgcgaaatgg
    14521 agattgatcc aaatggacaa gtagtctctc atgatatttt cccaagtgtg atccaaacaa
    14581 cagaacggat gacctacaca gctgtcaatc aaatcttaga agatcaagac gaagaaacga
    14641 tgcaacgcta tgctcagctc gtacctatgt tccaagaaat gggtgaattg caccgaatcc
    14701 ttgaaacgat gcgaatcaaa cgtggcgcga tttcttttga ggatcgcgaa gcgaaaatcc
    14761 ttgttgatgc tgaaggacac ccaaatgaca tccagctaag aagcagagga attggtgaac
    14821 gcttgatcga atctttcatg cttgcggcca atgaaacggt agctgaacat tataataaat
    14881 taaatctgcc gtttatttac cgaatacatg aacagccaaa agaagaaaaa atgcagcgct
    14941 tctttgattt tgcttcagct ttgggcattc tggtccgagg aacgaaagga acgatcacac
    15001 caaaagattt acagcatgtg atcgaaggag tagaagacaa accagaagct gcagttatca
    15061 atactatgtt gctaagaagc atgcaacaag cacgctattc agaagacaat tttgggcatt
    15121 atggcttagc ggcagattac tacacacact ttacatctcc tattcgacgc tatcccgatc
    15181 taatcgtcca tcgtctgatc cgttcttaca gtgaagataa aagtgaaagc aatcaaagtt
    15241 cgtgggctgc ggaacttcct gaaattgcag atcatagttc taagatggaa cgtcgtgcag
    15301 tagaaacaga acgtgaagta gattccatga agaaagcaga attcatgatg gacaaagtgg
    15361 gagaagaatt cgatgggatc atcagctctg ttgtgaaatt cggcctgttt atcgaactgc
    15421 cgaatacagt agagggattg atccatatca atgaattgaa acaggactac ttccattttg
    15481 ttgaaaatca tttagctctt gtaggtgaaa gaactggtct gacttttaaa atcggacaga
    15541 aagtgcgagt aaaagtagtc aaagctgatc cagatgaacg agcagtagat tttgaacttg
    15601 tttccgcaga agaggttaca gcgttggaac ggccaaaaac acgaaatgga aaatcaggaa
    15661 aacgcgaagg aaaacaagca ggaaaacgcc cagaaagaaa tggcaagaag aacaaacgat
    15721 atagcgatag taagcaaacg ttcggtaaga aaaaagggaa gaagccattt tacaaaggtg
    15781 ttaaaaaaaa gaaaaagaaa aaataaaatg agttggagga aacagctatg ccaaaaggcg
    15841 agggaaaatt aattgcacaa aacaagaaag ctcgccatga ttattcgatc atcgacacga
    15901 tggaagcagg gatggtcttg caaggaaccg agatcaagtc gatacgaaac agccggatca
    15961 atctaaaaga tggatttatt cgcgtccgca acggagaagc tttcttgcat aatgttcata
    16021 tcagtcctta tgaacaagga aatattttta atcatgatcc gttgcgcacg agaaagttat
    16081 tattacacaa aaaacaaatc atccggcttg aaaatgaatt gaaaaatact ggaatcactg
    16141 ttgttccttt aaaagtctat attcgtaacg gctatgccaa ggtattgatt ggtctggcga
    16201 aagggaaaaa atcttatgat aaacgggaag atttgaaacg aaaagatatc gatcgacaaa
    16261 ttgatcgaac attaaaaaat ttctctagat aatttcatta tcctttttcg catattcggt
    16321 gcttttgctt ttacttaatg ataaaatttg ttagcattta atctgataaa acatgattat
    16381 cataggtttt ttcttagatc aggagcaccg tgtataaaga aaaattcgct tcgtttttga
    16441 ctgaaataaa aaagtgtcta attttcatga aaatgttttg aattttctgt ttcatggtgg
    16501 tatgataccg tgtggttaga aaccgattgg aaaaaaccag atacgcacat gaattgattt
    16561 tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg aacgctcgct aacgttccac
    16621 attggaccaa tttggtttga tggtacagtt tgtataatgg tgttactgac ctgtttgatt
    16681 gttttcttct tagcttactt ttttacaaga aatcttaagt tgaaaccaac aggcaaacaa
    16741 aatgcgatag aatgggtcat tgattttagt cgaggaatcg taacagataa tctgcctaga
    16801 aaagaattga gcaatttcca tttgttggca tttacattgt ttttatttgt ttttgttgca
    16861 aacaacatcg gtttagtaac aaaaatcgtt ttacctaacg aaacaacact ttggaaaagc
    16921 ccgacagcag acccgtttgt tactttaact ttggcattta taatgatctt acttactcac
    16981 ttatttgggg tgaaaaagtt aggcttcaaa ggatatttcg taaattcgtt cttgaaacca
    17041 tatagtttta tgtttccgat gaaactgatt gaagaattta caaacttatt aacacttgct
    17101 ttacgtcttt atgggaatat ttatgcgggg gaagttttgc taaccttgat tgcaaaaatg
    17161 gtaataagct taggctggtt ctcattgccg ttagcaattc cattagagat ggtctggatc
    17221 gcattctcct tgtttatcgg aagcatccaa gcatttgtct tcgttacttt atctatggtt
    17281 tatatgagcc ataagattga agtggaagaa taatattcgt tttaaaaaca aactactatt
    17341 tatctaattt aggaggaaaa caattatgaa ttatatcgca gcagcaatcg caatcatggg
    17401 agcagcaatc ggagccggtt atggtaacgg tcaagttatc tctaaaacaa tcgaatcaat
    17461 ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca atgtttatcg gggtagctct
    17521 agtcgaagct gttcctatct taggggtagt tgtcgccttg attctagttt ttggtgttta
    17581 attgaatcag tattaaggca gaaacaattt ggtttgttac tgctttttta catgaaaact
    17641 agaagtactt caaagaaagg aaggctcgcc tatgctgaat caattggcaa ttgcagaagt
    17701 tggcaatgcg atgttaggaa atgtcatcgt tgtcagcggc tcatttttga tactactggc
    17761 tctcttgaag cattttgctt ggggaccaat cagcgatatt ttgaaaaaac gtgaagacaa
    17821 gatcgccaat gatttggatt ctgcagaaca atctcgcatc aactcagcga aaatggaaca
    17881 agaacgcgaa caacaattgt tagcctctcg ttctgatgca gctgatatca tcaaaaatgc
    17941 gaaagaaagt ggagaattaa gccgccaaaa tattttgaag gatgctcaag aagaagcagc
    18001 tcgtctaaaa agtaaagccc aagctgatat cactgtagaa cgtgattcag cgctgaactc
    18061 tgtaaaagac gatgttgcag aactatctct tcaaatcgcg gaaaaaatct tgaacaaaga
    18121 attatctcca gaaatgcacg aatcattaat caatcaatat atcgaaggtc taggttcttc
    18181 aaatgaaact agataaatat acagtaggaa gacgttacgg gaaagcttta tttgaactgg
    18241 cgatcgattc aaatcaagca gaagaaatct atcaagatct cgtaagctta cgtcaaatct
    18301 atgaacaagt acctggatta ggtaatatgt taagtgatgt tcgtctagaa cctcatgaaa
    18361 aacgagaaat catggacaag cttgttagtg gctatgatgg aatcgttaaa aactttttag
    18421 aagttgttta tgcgtataac cgaatggatg atcttttgtt catgatcgaa gaatatgaac
    18481 gtcgatatga tgaacatcaa ggtttgctgt tgggaagtgt cacaacagca gttccacttt
    18541 cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa aacaatgaac tatcaatcag
    18601 tcgaattaaa acaaatcgtt gattcttcta ttattggtgg cgcgattgtt gaagctaacc
    18661 atcgagtaat cgacggaagc attcgcacgc aattagaaaa tatgcgtaat caattaaata
    18721 gatagtacaa agaggtaggt gaatcgaatg gccatcaaag cagaagaaat tagtgccttg
    18781 attaaagaac aaattgaaaa ttatcaaaac gtgctttcag tcgaagaaat cggtaccgta
    18841 acttatgtag gtgacgggat cgctcgtgca catggtttag aaaacgcgat gagtggtgag
    18901 ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa acttagaatc aaatgatgta
    18961 ggtatcatta tccttggcga tttcgagtcc attcgtgaag gagataaagt aaaacgaaca
    19021 ggaaagatca tggaagttcc agttggagag gccttgatcg gacgggtagt taatccgcta
    19081 ggtcagccaa tcgacggact aggtgaaatc gttactgaca aagctcgtcc agtagaagca
    19141 atggcaccag gcgttatgca acgtaaatct gttaacgaac caatgcaaac aggtctaaaa
    19201 gcaatcgacg ctcttgtacc aatcggacgt ggccaacgtg aattagttat cggtgaccgt
    19261 aaaacaggga aaacttctat tgcgatcgat acgatcatca accaaaaagg tcaagatatg
    19321 atctgtatct atgtagcaat cggacaaaaa gattctacag ttcgtacaca agttgaaaca
    19381 ttgaaaaaat atggcgcaat ggattacaca atcgttgtga atgccggtgc gtctcaacca
    19441 gcgccgttgc tttatatcgc accatatgct ggtactgcaa tgggtgaaga gttcatgtac
    19501 aacggtaaac atgtcttgat catttttgat gatctttcaa aacaagctgt tgcttatcgt
    19561 gaactttcct tgctacttcg ccgtccacca ggtcgtgaag cttatccagg ggatgttttc
    19621 tacttacact cacgcttatt ggaacgtgca gcaaaattaa gtgatgattt aggtggcggt
    19681 tcaatgactg ccttaccatt tgttgaaaca caagctggag atatctctgc ttatatccca
    19741 acaaacgtta tttcgatcac tgatggacaa atcttcttag aaagcgactt gttctacgca
    19801 ggtactcgtc cagcggttga tgccggacta tctgtatctc gtgtaggggg ttctgcgcaa
    19861 atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag acttagcaag ttaccgtgaa
    19921 ttagaagcat ttacacaatt cggttctgac ttggatgcag caacacaagc aaaactaaat
    19981 cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc atgcaccgct agctgttgaa
    20041 aaacaagtag tgattttgta tgctttgaca catggattct tagatagtat ccctgtagac
    20101 agtattttag acttcgaaca tgaattattc gaatatctgg ataccaatca ttcagatatc
    20161 tttgaaacaa tccgtacaac aaaagatctt ccagaagaag aagcgttgaa cagtgcgatt
    20221 caggaatata aagacatgtt cttagctaca aaaggaaata attcctctac agaagataaa
    20281 ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg ggtgcttcat taaatgaaat
    20341 caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc acgcgggcaa tgcagatggt
    20401 gtctgcttct aagttgacaa aatctgaagc ttcttcacag aagtttcagg tttatgcaac
    20461 aaaagtaaga gaaatcgtta cgcatttgac tgctactcag ttaaatgata ttgcctcaga
    20521 aaatcctaga ggggatatca attacaacag catgctgctc agccgtcctg ttaagaaaac
    20581 tggttatatc gtcatcactg cagatggcgg tttagtcggt ggatataaca gctcgatatt
    20641 gaaacaaacg atgtctattt tagaagaaga ccaccaatca cccgatgatt acgtgatgat
    20701 cgcaattggc ggaactggtg cagacttctt taaagcacgc ggtatcaatc tagcatatga
    20761 gctgcgcaat ctatctgatc agccaagctt tgatgaagtc cgcaaaatcg taaatatggc
    20821 aacaactatg taccaaaacg aagtattcga tgaattatac gtatgttata accatcatat
    20881 caattcgcta actagtcagt ttcgtgtaga aaaaatgctg ccaatctcag acttagatcc
    20941 agaagaagca actactttcg aacaagaata tatttttgaa ccttcaaaag aagaaatctt
    21001 gtctcagtta ttgcctcagt atgctgaaag tttgatctac ggtgcgatcg ttgacgccaa
    21061 aacagcagaa catgctgctg gtatgacagc aatgaaaaca gcaactgata atgctgctaa
    21121 tatcattgat gagctgacgg tttcttataa ccgtgcaaga caaggggcta ttacccaaga
    21181 aattaccgaa attgttgccg gagcttcggc actagaatag tattgagatt ggaggaaaaa
    21241 agatgagttc aggcaagatt gttcaagtaa tcggtcccgt tgtcgacgtg gaattttctt
    21301 tagaccaatc cttaccagat atcaacaacg cattagttgt ttataaaaat gacgaaaata
    21361 aatcaaaagt tgttcttgaa gcagcgttag aattagggga cggagtgatc cggaccattg
    21421 cgatggaatc aacagatggt ttacaaagag gaatggaagt cattgataca ggcaaagcaa
    21481 tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt caacgtacta ggagatacga
    21541 tcgatttaga aacacctttc ccagaagatg cggaaagaag cgaaattcat aaaaaagcac
    21601 cagcctttga cgaattaagt acaagtacag aaattttgga aacagggatc aaagttatcg
    21661 atttgcttgc cccatattta aaaggtggga aagtcggact attcggtggt gccggtgttg
    21721 gtaaaaccgt actgatccaa gaactgatcc ataatatcgc ccaagaacat ggtggtattt
    21781 ctgtatttac tggtgtaggt gaacgtactc gtgaaggtaa tgacttgtat tatgaaatga
    21841 aagattcagg agttatcgaa aaaacagcca tggtgttcgg acaaatgaac gaaccaccag
    21901 gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc ggaatatttc cgtgatgtag
    21961 aaggtcaaga tgtactgttg tttatcgaca acattttccg tttcactcaa gctggatctg
    22021 aagtatcagc cttgttagga cggatgcctt ctgcggttgg ttatcaacca acattggcaa
    22081 cagaaatggg tcaattgcaa gaacgtatca catctacgaa aaaaggttcg atcacatcga
    22141 tccaagcaat ctatgtgcca gccgatgact acactgaccc ggcgccggca acagcattcg
    22201 cccatttgga tgcgacaacc aacttggaac gtaaattaac agaacaaggg atttatccag
    22261 cggtagatcc attggcttct tcttcaagtg ctttagcacc tgaaatcgtg ggagaagaac
    22321 attataaagt agcaacagaa gtccaacacg tattgcaacg atatcgtgaa ttacaagata
    22381 tcattgctat cttaggtatg gatgaattat ccgatcaaga aaaagtgtta gtttctcgtg
    22441 cccgcagagt ccaattcttc ttgtcacaaa actttaacgt ggctgaacaa tttactggtc
    22501 ttccaggttc atatgtccct gtagaagaaa cagtgaaagg attccgcgaa atcttggaag
    22561 gcaaatacga tgatcttcct gaagaagctt tccgaagtgt tggccgaatc gaagacgtcg
    22621 tagaaaaagc gaaaacacta ggctactaga aaggggtgcc ccatggatca atatttaaca
    22681 gtaaatgtgg taactcctgc cggtttggtc tatgatcacc atgcagctat cgtcgttgca
    22741 cgtacgacag ctggtgagct tgggattttg cctaaacatg caccaatcat cgttccattg
    22801 acgattgatg aagttcgtgt aaaacgaaca gattcagata cccacgtcga ttggatcgct
    22861 gttaatggcg gaatcatgga agttcgtgat aatgttgttt caatcgttgc tgatagtgcg
    22921 gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg caaaacaacg cgcagaacgc
    22981 caaatcgaag aagcaaaagc aaaacaagat acgaacgaat tgaaacgggc aactgttgcg
    23041 ttgcaccgag caatcaatcg tatcaaagta tctaagcact cataaaatga gatgcggatg
    23101 tttatccgtg tttaagaaca gtaaaaagag gttatgacag aagtggtcag cttcaagaca
    23161 taagaaggaa tttacgaaaa ttgcttctca aatttttgtg aaatctcagc ttatttttcg
    23221 aagaagctac ttctggaaca ccgtatatca gaggttgtaa aatcagcgtt ttgtcctgag
    23281 tattagagaa acaaatggaa aagagttcct catattttgc cgttttttga gctaataccg
    23341 agggtcagat gattgaacac cgtttatt
//
LOCUS       DACCCM010000045        22856 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCCM010000045 DACCCM010000000
VERSION     DACCCM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.047"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(104..1336)
                     /locus_tag="IVK78_001700"
     CDS             complement(104..1336)
                     /locus_tag="IVK78_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6375174.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IVK78_001701"
     CDS             1591..2160
                     /locus_tag="IVK78_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6375175.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IVK78_001702"
     CDS             complement(2338..2778)
                     /locus_tag="IVK78_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP6375176.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IVK78_001703"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IVK78_001703"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP6375177.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IVK78_001704"
     CDS             3732..4655
                     /locus_tag="IVK78_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP6375178.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IVK78_001705"
     CDS             4731..5870
                     /locus_tag="IVK78_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP6375179.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IVK78_001706"
     CDS             5863..6663
                     /locus_tag="IVK78_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6375180.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IVK78_001707"
     CDS             6663..7490
                     /locus_tag="IVK78_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6375181.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IVK78_001708"
     CDS             7468..8202
                     /locus_tag="IVK78_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6375182.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IVK78_001709"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IVK78_001709"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP6375183.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IVK78_001710"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IVK78_001710"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP6375184.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IVK78_001711"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IVK78_001711"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP6375185.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IVK78_001712"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IVK78_001712"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP6375186.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IVK78_001713"
     CDS             11787..13820
                     /locus_tag="IVK78_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375187.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IVK78_001714"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IVK78_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IVK78_001715"
     CDS             15353..16159
                     /locus_tag="IVK78_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6375188.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IVK78_001716"
     CDS             16171..17391
                     /locus_tag="IVK78_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375189.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IVK78_001717"
     CDS             17381..18967
                     /locus_tag="IVK78_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6375190.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IVK78_001718"
     CDS             19006..21144
                     /locus_tag="IVK78_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6375191.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="IVK78_001719"
     CDS             complement(21512..22495)
                     /locus_tag="IVK78_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP6375192.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4711 g         7070 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgt
//
LOCUS       DACCCM010000046        22453 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCCM010000046 DACCCM010000000
VERSION     DACCCM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.048"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            194..2728
                     /gene="secA"
                     /locus_tag="IVK78_001720"
     CDS             194..2728
                     /gene="secA"
                     /locus_tag="IVK78_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP6375193.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2854..3955
                     /gene="prfB"
                     /locus_tag="IVK78_001721"
     CDS             join(2854..2916,2918..3955)
                     /gene="prfB"
                     /locus_tag="IVK78_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP6375194.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4060..4746
                     /gene="ftsE"
                     /locus_tag="IVK78_001722"
     CDS             4060..4746
                     /gene="ftsE"
                     /locus_tag="IVK78_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP6375195.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4730..5623
                     /locus_tag="IVK78_001723"
     CDS             4730..5623
                     /locus_tag="IVK78_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6375196.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5775..7223
                     /gene="cls"
                     /locus_tag="IVK78_001724"
     CDS             5775..7223
                     /gene="cls"
                     /locus_tag="IVK78_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6375197.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELSEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7560..8417
                     /locus_tag="IVK78_001725"
     CDS             7560..8417
                     /locus_tag="IVK78_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP6375198.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8532..9452
                     /gene="pstC"
                     /locus_tag="IVK78_001726"
     CDS             8532..9452
                     /gene="pstC"
                     /locus_tag="IVK78_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP6375199.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9452..10336
                     /gene="pstA"
                     /locus_tag="IVK78_001727"
     CDS             9452..10336
                     /gene="pstA"
                     /locus_tag="IVK78_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP6375200.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10351..11157
                     /gene="pstB"
                     /locus_tag="IVK78_001728"
     CDS             10351..11157
                     /gene="pstB"
                     /locus_tag="IVK78_001728"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6375201.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11173..11931
                     /locus_tag="IVK78_001729"
     CDS             11173..11931
                     /locus_tag="IVK78_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6375202.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11944..12621
                     /gene="phoU"
                     /locus_tag="IVK78_001730"
     CDS             11944..12621
                     /gene="phoU"
                     /locus_tag="IVK78_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP6375203.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12858..13889
                     /locus_tag="IVK78_001731"
     CDS             12858..13889
                     /locus_tag="IVK78_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6375204.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13886..14653
                     /locus_tag="IVK78_001732"
     CDS             13886..14653
                     /locus_tag="IVK78_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6375205.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14753..16321
                     /gene="liaX"
                     /locus_tag="IVK78_001733"
     CDS             14753..16321
                     /gene="liaX"
                     /locus_tag="IVK78_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP6375206.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16621..16938
                     /locus_tag="IVK78_001734"
     CDS             16621..16938
                     /locus_tag="IVK78_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP6375207.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            16938..17288
                     /locus_tag="IVK78_001735"
     CDS             16938..17288
                     /locus_tag="IVK78_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6375208.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17455..18393
                     /locus_tag="IVK78_001736"
     CDS             17455..18393
                     /locus_tag="IVK78_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP6375209.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18408..19238
                     /locus_tag="IVK78_001737"
     CDS             18408..19238
                     /locus_tag="IVK78_001737"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6375210.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19282..20307
                     /locus_tag="IVK78_001738"
     CDS             19282..20307
                     /locus_tag="IVK78_001738"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6375211.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20322..21260
                     /gene="galU"
                     /locus_tag="IVK78_001739"
     CDS             20322..21260
                     /gene="galU"
                     /locus_tag="IVK78_001739"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6375212.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21385..21825
                     /locus_tag="IVK78_001740"
     CDS             21385..21825
                     /locus_tag="IVK78_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP6375213.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21828..22430
                     /locus_tag="IVK78_001741"
     CDS             21828..22430
                     /locus_tag="IVK78_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6375214.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         7441 a         3855 c         4900 g         6257 t
ORIGIN      
        1 ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa
       61 atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa
      121 ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga
      181 gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact
      241 aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt
      301 aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga
      361 aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt
      421 attaggttta tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa
      481 tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct
      541 aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg
      601 tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat
      661 caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac
      721 gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat
      781 ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga
      841 agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag
      901 agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc
      961 aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga
     1021 taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc
     1081 caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt
     1141 tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc
     1201 aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac
     1261 tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac
     1321 agaagaagaa gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg
     1381 tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca
     1441 cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac
     1501 tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca
     1561 tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca
     1621 aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg
     1681 acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg
     1741 tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca
     1801 gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc
     1861 tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac
     1921 gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa
     1981 cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca
     2041 agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac
     2101 aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaactgga atttggaaag
     2161 tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc ttgcagatat
     2221 cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt
     2281 taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt
     2341 gatcttgcgt gttgtggact ctaaatggac agaccatatt gacgcaatgg atcaattacg
     2401 tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga
     2461 aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt
     2521 tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc
     2581 tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat
     2641 ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa
     2701 gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct
     2761 ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta gactagatat
     2821 aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc
     2881 ctgacagaaa tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct
     2941 ggaagaagac atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag
     3001 cgaagccgca caaaaagtga tcagtgaaaa taacgctaat aaagaaacct atgaccaatt
     3061 ccattctctt gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga
     3121 acctgatgca gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat
     3181 gaagacgtat gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt
     3241 cgaattgcat ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg
     3301 tatgtataca cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc
     3361 aggtgatgaa gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg
     3421 ttatctaaaa tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc
     3481 gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat
     3541 cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg
     3601 tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac
     3661 agtagtagct agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat
     3721 gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact
     3781 tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc
     3841 ctattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat
     3901 ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc
     3961 cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct
     4021 ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta
     4081 atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa
     4141 ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg
     4201 atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata
     4261 gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac
     4321 tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc
     4381 ggcagaaaac ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg
     4441 aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct
     4501 attgctcggg caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat
     4561 ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga
     4621 actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta
     4681 attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat
     4741 gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg
     4801 gatgacagtt gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat
     4861 ggcagttatc atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc
     4921 tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa
     4981 tgacttagat catgttaaaa aagttgaatt ttcaagtaaa gaacaggaat tgaaaaaaat
     5041 ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga
     5101 tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc
     5161 agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca
     5221 aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc
     5281 gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca
     5341 aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg
     5401 tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat ttggttatgc
     5461 atggtttttc caattgatca atccatcatt attacgttcc cattattcat tgattcatcc
     5521 acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc
     5581 acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa
     5641 aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa
     5701 ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagag
     5761 gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga
     5821 atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg
     5881 catggctgct cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag
     5941 gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga
     6001 acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta
     6061 ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct
     6121 atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga
     6181 ttgaagatat caagcaagca gaagaccata tccacttcca gtattatatt tatcgcagtg
     6241 atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag
     6301 tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt
     6361 tgaaaaaagc aggcggagaa atcgcatttt tctttcctct atttgttccg tatattaatc
     6421 ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa atcggatata
     6481 caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc
     6541 gagacaatca tttgcgtatt tacggagaag cggtgtatat cttgcaaaac cgttttttga
     6601 tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca
     6661 ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg
     6721 aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc
     6781 aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt
     6841 caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg
     6901 caacgtattc tttcgctgca gagctgagcg agtatggagc aaggatcgaa acatatgaga
     6961 atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag
     7021 caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg atttatgatg
     7081 aacgaatggc ttcacgtgta agaaaagcat tttttgaaga ctcgaaaatt tctacgcatc
     7141 tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa
     7201 gattgatttc gccgctcctt taaaacacaa aaaggacatg agaaccatca acagatggag
     7261 atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt
     7321 ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa
     7381 gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag aaacttttaa
     7441 tgagatagtt ttttttacaa ttatgaggat ttattttgtt atttaatgat tttgattcgt
     7501 tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg agatttctaa
     7561 tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta tcaggctgtt
     7621 caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct ttacagcctt
     7681 tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc atcaatgtac
     7741 aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc gacatcggaa
     7801 actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta gtcgatcatc
     7861 gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta aaagacatca
     7921 gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt gaagtcggtg
     7981 gaaaagatca gccagtagtt atattgaacc gtgcttcagg aagcggaacc cgtagcacgt
     8041 ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa gattcaagcg
     8101 gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt gctttttctt
     8161 atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca gatgaaaatg
     8221 tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga gatcatccaa
     8281 aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa gataacatcg
     8341 ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat tggcaaggaa
     8401 atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact tttacatgtt
     8461 attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac ctgattaagg
     8521 gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa cgttcaaaaa
     8581 tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg ttggttgtat
     8641 tatctatttt ttattttgta gctagtaaag gattggccac cttttttact gataagatca
     8701 atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga gcaaatggga
     8761 aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc tttttatctg
     8821 ctattgttgc cacaccattt gccattggtg cagcagtatt catgacggag atctctccta
     8881 aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt ccttctgtcg
     8941 tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt tttggcggga
     9001 cagggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg ccgactgtca
     9061 caacgatgac tgttgatgca ttaaaggcag taccacggca ctatcgggaa gcttcattag
     9121 cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca gccgtgcccg
     9181 ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc ttagctatcc
     9241 aaatggtcat cggtaatgca gcattgatgc caacaggact tactactcct gcttctacat
     9301 tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt gaaaataatg
     9361 tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat atcgtgatcc
     9421 gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag ataaaattgc
     9481 tacaggtgtt ttatatgtag tttcggggat catcgtattg attttagcag ctttactact
     9541 ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg aaccttccaa
     9601 agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt atttgctttt
     9661 gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc tttcagaata
     9721 tgctggaaaa aactggctga cagatgtgat tcgcacgtcg attgaaatct tgagctcatt
     9781 gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat tccagtatgg
     9841 tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc ttttgactag
     9901 aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag gattggcttt
     9961 aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga caccagggat
    10021 tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg cattgatcta
    10081 tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat taagtgtttc
    10141 aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt ggaagatcaa
    10201 tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg cagtgttgat
    10261 cattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat tataccgaaa
    10321 aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa tgaaacaaat
    10381 gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct acatgtctgg
    10441 tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa taagatcact
    10501 gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt gaatcgaatg
    10561 aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg cgtagatatt
    10621 aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt ttttcaacgt
    10681 cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa acagcacggc
    10741 gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca agctgccttg
    10801 tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg cgggcaacag
    10861 caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct gctagacgag
    10921 ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact tgtaaacttg
    10981 aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcggc tcgtatcagc
    11041 gactatactg cctttttcta tctaggaaaa gtaatggaat atgacaaaac taacaagata
    11101 tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt tggttaagac
    11161 ggaggcctca acatgacaaa agaaattatt tcatctaaag atcttcatct gtattatgga
    11221 aagaaagaag cactaaaagg gatcgatcta agtttcaatc aaggggaaat cactgcgatg
    11281 atcggtcctt ctggttgcgg gaaatccact tatctgcgtt ctttgaaccg gatgaatgac
    11341 ctgattccga atgtaacgat caccgggagt gttttatata aagacaaaga catctatggt
    11401 ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca gcagccaaac
    11461 ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa aggggaaaaa
    11521 gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc tgtttgggat
    11581 gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca gcagcagcgt
    11641 gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga tgaaccaaca
    11701 agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga attaagagag
    11761 cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat ttcagataag
    11821 acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa gatattcatg
    11881 aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata aggaggaaac
    11941 ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca gttctacgaa
    12001 atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat caaacacgat
    12061 aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat ggaaacacgc
    12121 ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac tgatttgcgt
    12181 atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga tcatgcggtg
    12241 tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga aatcgaaaaa
    12301 gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct agttgcttat
    12361 gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag agtcaatgac
    12421 tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc tgagacagtc
    12481 atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg agactatgtg
    12541 acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga gttaaatacg
    12601 aaccacaatg agattttcta aactaaagcg gtgattctgc cgtttgttta ggaaaagagg
    12661 ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagtcaaaca
    12721 aaaatagctc ctcatatttt tcatttggct ggacttatta ccgcagatgc aagcttttga
    12781 acactgttta ttcgggaaag ggtttgtgac aaaatatgtc acaaaccctt ttcatcattt
    12841 catagaaagg ataaagaatg caaaaaaaaa ttagcatcat cactatattt tttatttgtg
    12901 tgcttacttc atgggttatt cagcatcaag ctgctttata tgaagtacct gtcatcgaga
    12961 tcgaaaaagt acgaataaaa gaggatcatc aattgattac aggaagactg atgaacacga
    13021 atcaaaaagg agaatcgatt tatttatcgg ttccttatca aaaaaatcaa atagaaacag
    13081 caaatataaa agcgagacaa atgtatctag tccaaatgca tggagatgaa tggcagctag
    13141 tagcaaaaaa aaatgatggc tggcttttct tcttcacaag tatcttcatt ggcgccatgt
    13201 tgcttattgg cggaaaacat ggtctatttg ctttaggaag tctcatcatc aatatcgttt
    13261 tacttttact tttgttgctc atctttacta aaatagaagg gatttctcta ttaaatatat
    13321 ctatcctgtt cgtcgttata gggatgatta ttagcatatt ggctttagat ggatggaata
    13381 gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt ttttttagca ttttttattt
    13441 gtcttttgac aatgaatctt tggaaagacc aaggactgcg ctatgaggaa ctgaattatc
    13501 tgacacgacc gtatcgttct gtttttcttt ctagcgtact gattggaaca gctggaggaa
    13561 cactcgatac cgttatcaca gtcattgcga cattagaaga attgactaag cgacaaccag
    13621 aaataacagc gaaacagtta tggctttcgg gaaggcaagt gggtcgagat gtgattggct
    13681 cgatgaccaa tatccttttg ttcgtctata tcagtgggtc agttccatcg cttcttttat
    13741 atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca gcatttatca ttggaatttt
    13801 tacgcgtcat tgctggcagc ttaggaatag tattatcgat ccctatctcg gtcctctttt
    13861 ttctgactgt aaggaggctg aaacgatgaa tgtacttatt atacttgttg ggattttagc
    13921 aatcagcgtg ctacttattg gcggtacgca aggtatgtat attcttttag gcttattcat
    13981 caatttaggg atctttttct tgttgttatt cggttatcat cagaagtggc cgatactggc
    14041 attgtcggtc ataggatttt tattgattgc tgtggttatc ttatttttta tcaacggtta
    14101 taatctaaaa atgcgtgcag cttttgtgag tatactgatc tttttatttt gtttcctact
    14161 attgcttcct atcactgatt ttttagcaat ccaaggtttt acaacgatag aactagaaga
    14221 attatctggc ttagataaga ctttagcgat tgattttaga ttacttacga gatcgttgct
    14281 tctgattagt cttagtggtg ctgttttaga tgcgagtgta gcaatttcta gtgggacatt
    14341 cgaagtatat caggcaaatc cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc
    14401 tattgctaaa aaaatattgg cgtcaacagt aatgacactt ctttttgcat tcatgggaag
    14461 ttctttagcg ttgatcctct ggtttattga tttagacatt ccttttcagc aagtgatcaa
    14521 tgaaaaatca tttgttctag aatatacgat ggctattctt accacattgt ccgcactact
    14581 tgttcttcct ttaacctgcg tgactacagc atatctcttt acaaaaaaga aagaacaact
    14641 caagatggag taaaagttac aacttaagtc ctatgacatt tttactgaat cagtgcataa
    14701 tatggatgta aggaaaagct tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga
    14761 aagagaacgt attttagatt tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga
    14821 tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa atcaaaaaag aagcagacaa
    14881 ggtaactgct agtcataaag aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa
    14941 cggcgaagaa gaaatttctg agccagttga tccaaaggaa agagaaagac aagatcaaga
    15001 aaacttagaa aagatcttag atgaactagc aactgaagcg aataaaactt cggcgcgtct
    15061 agatgaagta aatgctcaat tcgctgacaa taaggcagca cgcaatgaga aacaggaagc
    15121 attgatgcag ctgaatacaa aagaagaatt aggcgagttg acagaagaag aattagcaca
    15181 gcgtcagacg ttagaagcag aaatcaaaga gttagaagct agtggcgata cactgttaga
    15241 agaacaattg aaattagaag cagaattaaa agatatccgt aagaatcaat ggtctgagaa
    15301 aaaagaaacg tttactgata agtttgaatt gccagatgat tggaaagatc aagcaacaga
    15361 tacactgaat caagtaggcg aaaaaatgtc agaagctgga tcacagttag gaaaattctt
    15421 gaagaaaaca ttccaaactg tttcagaaac agtgaatgac aacatggaat ggaaagatgt
    15481 cagcttgcgt gtccctggga ttgcgacaac gaaatttgag catgaatttt actatgaagc
    15541 tcctgcagca agtatccttg atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg
    15601 ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga
    15661 accatttgaa gcattctctg aacggagcca gattgaggtt aatgaagatc atatctcctt
    15721 ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc tacttaccta aacgagtata
    15781 cgaccacgca gctattaaac ttctaaatgg caacatcatg attgaaacat tggaagctaa
    15841 agatatttat acaaaatcta cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat
    15901 gctggaagta gaaggtgtaa acggcaatat cgatattcga aacgggaaca tcttagattc
    15961 tattatcgaa acagtaaatg gtacagtgac atttggtgcg acagcagaaa acctttctgt
    16021 gtctttggta aatggtgatg tacgtcttac gattaaagaa gataatttga aaaaagtaga
    16081 agcaagttca gtgaacggaa atgtcaaagt cgctttacca gatggcatcg gtttggaagg
    16141 acatgctaaa acaagtcttg gaagtatcaa cagccgtctg tccaattacg aagtagttcg
    16201 tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt cgtgtttcag atggtgaaat
    16261 cgcgcaagtg caactttcaa cgacaacagg aagtatctat ttaaaagata cagataaata
    16321 aagaggttgt gaaagagatg ttcagccttg agcaataaga agcgattttg taaaatagct
    16381 tcccctattt tacaaaattg tgccttattg ccgataggct atctcttgaa caccgtttac
    16441 caagagatta tgaacttgcc aattatcgcc gagtcacaag gaataatttc aaaaaaagct
    16501 cgtcacgttt tatgaatatg gtaatttgtg accgagaatt ttgcaaaaag acaccgatta
    16561 agaagaaggt tgtcacaacg attttcttca tttaaaatag tagaacagag gtgagacatg
    16621 atgaaaaaaa agttgacaaa atccaataaa aatgttgttt tgacagggac tttagcagga
    16681 ataggagaat acatcgggat tgatccgact gttatccgtg tgctttatgt attcctgagc
    16741 ttagttgctt ttggaagtcc aatcatttta tacattttat tagctttgat tatcccatct
    16801 gcgcgttctg cacgaacaaa ctatggacat aacaatgcat attatgaaaa aaacaattat
    16861 cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac
    16921 gaatggagtg acttttaatg tcgtactttc aacgtatttt agttaatacg ttaacattca
    16981 tatcattggc tgttcttttt cctgcgatgg tccatgtgag cagtatttgg acggccgttc
    17041 ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa accgattttg acgatactgt
    17101 ccctgccctt tacattagtc acattgggat tgtttacgtt tgttatcaat gcagcgattc
    17161 ttaaaatgac ctcctttttt gtgggagcag aacattttag tttttcttca ttcggtgcag
    17221 cgttgttagt agctatcttg atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta
    17281 gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt ttttatttag aatcaggaat
    17341 gcgttttcta ttacattggt aattaaataa aaacgttttt tttaattggt agtacttttg
    17401 gctagacaat gataaaatga tagagaacac tctaatagaa atgaggtagc atgaatgtca
    17461 gaagttgtaa aagttagcca attagtgaaa aaattatctt tggaaatcgt gacaggcgac
    17521 gagaaaagct tagaccgtgt catcgtaact ggggacatct cacgcccagg actagagttg
    17581 actggttatt ttaattatta ttcccataat cgtttacagc tatttggcag taaagaaatc
    17641 agttttgcgg aacggatgat cccagaagag cgtttgatca tcatgagaag attatgtagt
    17701 gaagatatgc ccgcatttat tatttcaaga ggattaccag ctccagaaga aatgatccag
    17761 gcagcaaatg aaaatgggat agctgtactg cgttctccca tctcaacttc tcgcttatta
    17821 ggagaattat ccagttattt agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt
    17881 ttagtggatg tttacggatt aggtgtactg attcaaggtg acagtgggat aggaaaaagt
    17941 gaaacagcgt tggagttgat caaacgcggt catcgattga tcgcagacga tcgagtagat
    18001 gtttatcagc aagatgaatt aacggtgatc ggagaaccgc ctaaaatcct ggaacatctg
    18061 attgaaatcc gaggaatcgg gatcattgat gtaatgaatc tttttggtgc gagtgcggta
    18121 cgtggctcta tgcaagtcca gttggctgtt tatctggaag catgggcaaa agataagaaa
    18181 tacgaccgtc tgggctctga agacacgacc gttgaaatcg cggaagtagg cattccacaa
    18241 ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca tcattgaagt agccgcgatg
    18301 aatttccgtg cacgtacaat gggctttgac gcaacgaaaa catttgaaga aagattaagt
    18361 cgcttgattg aagaaaactc aggcaacgac tagcaaggag tgacaatatg ttagcacaaa
    18421 tcaatcgagt tgcttttgag cttgtaggga ttcctatcta ctggtatgca ttgatcatcg
    18481 tatcaggtat cattatcgcc atgtggctta gcagcagaga ggctgttcgg gtaggattaa
    18541 aagcagatga tgtgacagat tttatgttgt ggggattgcc tatctccatt attggcgctc
    18601 gcttatatta tgttttattt gatcttcccc agtatatagc tgatccaatc caaatattca
    18661 atatccgttc aggaggctta gctatttatg gcggactgat tgcaggcgcg atcacgctgt
    18721 atttttttac taaatatcat tttatttctt tgtggacttt tttagatatc gctgcaccaa
    18781 gtgtattatt agcacaagca atcggtcgtt ggggaaattt catgaaccac gaagcatatg
    18841 gaccagacac gacgcgaagc ttcttagaaa gtctgcattt accgcaattc atcattgata
    18901 atatgtatat cgaagggatc tatcgtcaac caacatttct ttatgaatca ctttggagtt
    18961 tgattggatt tattttgctt ttgtttttac gaaagaaacc acaccttttg aaaaaaggtg
    19021 aagtcctttt atgttatcta atgtggtata gtttcgggcg tttctttatt gaaggattac
    19081 gtacagacag tctttacttt ttaggtgtga tccgtatttc tcaattatta tctgctgtgt
    19141 tatttgtcgg tacattgatt ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt
    19201 atgaccgctc attaggaaaa aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa
    19261 aaaaatttca ggagtggtaa aatgaattat agagtagcgg ttttaggacc aggctcttgg
    19321 ggtactgctt tggcacaagt cctcgccgaa aacggacatg acgttcggat ttggggacat
    19381 cgtgcttcac aagtagaaga aatcaacacc caacacacga atcaacgtta tttaccagat
    19441 cattgtctac ccgattccat ccaagcattc accaatatgg aagatgcagt aaaagacgca
    19501 gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg
    19561 cctaaattga atacaaagcc agtgatcatc catgcaagta aaggattaga acaggggaca
    19621 cataaaagga tctctgacat cttgttagag gaaatcccag tagaaaaaag aaaagctgtt
    19681 gttgttcttt ctggaccaag ccatgcagaa gaagtagcga aacatgacat cactacgatc
    19741 acagctgcaa gtacagatgc aaaagcagca aaatatgtac aagagctgtt catgaataat
    19801 tattttcgga tctataccaa tcctgatgtg atcggtgtag agatgggggc agctttgaaa
    19861 aatattattg ctattggtgc tggcgctatt cacggcttag ggtttggcga taatgcaaag
    19921 gcagcaatca tgacgcgcgg attagctgag atcagccgtt taggtgtagc aatgggtgcc
    19981 aacccgctga cctttatcgg attaagcgga gtaggcgatc tagtcgttac ttgcacaagt
    20041 gttcattcta ggaactggcg cgcaggcaac ttgttaggac aagggcagcc cctagaagag
    20101 gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata ctacccaagc agcgatggaa
    20161 ctagcccaaa taatggaaat cgaaatgccc atcactcaaa caatctatga agtgctgtat
    20221 gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt tacgtgatgg gaaaaaagaa
    20281 aatgaattct cattaacaat tttttgaagg agcgaatgta tatgaaagtc aaaaaagcag
    20341 ttattccagc agcaggatta ggaacaagat ttttaccagc aacaaaagct atggcaaagg
    20401 aaatgttgcc aattgttgat aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat
    20461 caggtatcga agacatcttg atcgttacag gaaaagctaa acgaccaatc gaagatcact
    20521 ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa aggaaagacc gaattattga
    20581 aattagtaga agaaacgaca gatgtcaact tgcatttcat ccgtcaatct catccaaagg
    20641 gtttaggaca tgccgttctt caagcaagag cctttattgg aaatgaacca tttgtcgtta
    20701 tgctaggcga tgatataatg gaagacgaag taccattgac acagcaatta atcgatgatt
    20761 acgaacgtac tcatgcttct acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa
    20821 aatacggaat tatcaatcca ggagaagtat tggaagatgg gttatacaat gtcaaaaact
    20881 ttgttgaaaa accagatcca agcaaagcac caagtgattt agctattatt ggacgttact
    20941 tattgactcc agaaattttt gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa
    21001 tccaactaac tgacgcaatc gatacattga ataaaacaca acgcgtattt gcccgtaaat
    21061 tcacaggtaa acgttacgat gtcggtgaca aatttggttt tatgaaaaca agtattgaat
    21121 atggtctaac acatccagaa gtaggtgcac ctttacgtga gtacatcctg tctttaggta
    21181 aaacattaga agcagaagga agcaagatcg atcataatcc aaaaggaata acagaaatca
    21241 aagaagaaaa gaaaaaataa cccttttttc taacgagctt cagctgctaa ttgaataaaa
    21301 agtagttgaa gctcgttttt atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa
    21361 gagagacaag aagggagttt gatcatgtca ggaggagaaa ttgcaggatt gatcgcagca
    21421 atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact
    21481 ttagaaaaag tagatcagac tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa
    21541 gacgtagatc ttttgtctag acaagtagaa ggcttactgg tcaaaagtaa tgaactgctc
    21601 ttggatatca atggaaaagt cgcaacgatc gatccattgt tcacagcagt tgctgattta
    21661 agtgaaagtg tatcagaact taatcattct agtaaaaaca tcgcgacgaa agtcggcgga
    21721 atcggtaaaa cgacagctaa agcgacggtt gccggaaaag tcggtgggac agcgatgaaa
    21781 ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa aataaaaatg gctaaaaaag
    21841 gtggattttt attaggtgca gtagtcggag gcacagccgc agcaatcgcc gcgttattgt
    21901 tagcaccaaa atctggtaaa gaacttcggg atgaattagc tgcccaagcg gatgatttga
    21961 tggatatggc ttcagattac acagatatgg cgaaagaaaa atcgactgag ttaacaggta
    22021 ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg tgatctagca ggtagtgtca
    22081 aacaaaaagt aagtgaatca gcagatgaag caggaagtga atctgacgat atcttgttaa
    22141 atcttaaaga acaatcagct gacttatcta atcgtttcaa aaaaacggca gaagatatga
    22201 aaaaacctgc agatgaatta ggaagtattg ctaaagatac ttcagaagat atcataattg
    22261 atgtaaaaga cgctgctggt aaagcaggag atactgtcaa tgaaggaatg tcagaagcaa
    22321 aaccagttgc agatgactca aaagaaatga tgaaaatggc aaaagaagat gcgaaagaag
    22381 ctaaaaaaga aataaaagat acaatgacag attcatctac aaatcactaa aatcacaaat
    22441 ttatagaata aaa
//
LOCUS       DACCCM010000047        22132 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCCM010000047 DACCCM010000000
VERSION     DACCCM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.049"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..523
                     /locus_tag="IVK78_001742"
     CDS             <1..523
                     /locus_tag="IVK78_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6375215.1"
                     /translation="GGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVA
                     NCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGS
                     LIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVL
                     LLPLFFQPFAGLF"
     gene            523..2109
                     /locus_tag="IVK78_001743"
     CDS             523..2109
                     /locus_tag="IVK78_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6375216.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2128..2349
                     /locus_tag="IVK78_001744"
     CDS             2128..2349
                     /locus_tag="IVK78_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP6375217.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2346..3107
                     /locus_tag="IVK78_001745"
     CDS             2346..3107
                     /locus_tag="IVK78_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP6375218.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3487..3996
                     /locus_tag="IVK78_001746"
     CDS             3487..3996
                     /locus_tag="IVK78_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6375219.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4071..4610)
                     /locus_tag="IVK78_001747"
     CDS             complement(4071..4610)
                     /locus_tag="IVK78_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP6375220.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="IVK78_001748"
     CDS             complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="IVK78_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP6375221.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5677..6474
                     /locus_tag="IVK78_001749"
     CDS             5677..6474
                     /locus_tag="IVK78_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP6375222.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            6502..7137
                     /locus_tag="IVK78_001750"
     CDS             6502..7137
                     /locus_tag="IVK78_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP6375223.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7157..7930
                     /locus_tag="IVK78_001751"
     CDS             7157..7930
                     /locus_tag="IVK78_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6375224.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8338..9135
                     /locus_tag="IVK78_001752"
     CDS             8338..9135
                     /locus_tag="IVK78_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP6375225.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9113..9526
                     /locus_tag="IVK78_001753"
     CDS             9113..9526
                     /locus_tag="IVK78_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375226.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9510..12155
                     /gene="mutS"
                     /locus_tag="IVK78_001754"
     CDS             9510..12155
                     /gene="mutS"
                     /locus_tag="IVK78_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6375227.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12173..14281
                     /gene="mutL"
                     /locus_tag="IVK78_001755"
     CDS             12173..14281
                     /gene="mutL"
                     /locus_tag="IVK78_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP6375228.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14303..14863
                     /locus_tag="IVK78_001756"
     CDS             14303..14863
                     /locus_tag="IVK78_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP6375229.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14948..16936)
                     /gene="treP"
                     /locus_tag="IVK78_001757"
     CDS             complement(14948..16936)
                     /gene="treP"
                     /locus_tag="IVK78_001757"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP6375230.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17159..17872
                     /gene="treR"
                     /locus_tag="IVK78_001758"
     CDS             17159..17872
                     /gene="treR"
                     /locus_tag="IVK78_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP6375231.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17949..18395
                     /gene="msrB"
                     /locus_tag="IVK78_001759"
     CDS             17949..18395
                     /gene="msrB"
                     /locus_tag="IVK78_001759"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP6375232.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18565..19167
                     /gene="ruvA"
                     /locus_tag="IVK78_001760"
     CDS             18565..19167
                     /gene="ruvA"
                     /locus_tag="IVK78_001760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP6375233.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19180..20181
                     /gene="ruvB"
                     /locus_tag="IVK78_001761"
     CDS             19180..20181
                     /gene="ruvB"
                     /locus_tag="IVK78_001761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP6375234.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20210..20788
                     /locus_tag="IVK78_001762"
     CDS             20210..20788
                     /locus_tag="IVK78_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6375235.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20840..21322
                     /locus_tag="IVK78_001763"
     CDS             20840..21322
                     /locus_tag="IVK78_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP6375236.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21439..21813
                     /locus_tag="IVK78_001764"
     CDS             21439..21813
                     /locus_tag="IVK78_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375237.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7237 a         3991 c         4790 g         6114 t
ORIGIN      
        1 cgggggcaat tttttgacta tgctgattac tcctgcgaca gtggcacttg ccatccctct
       61 ttataaaaac ttccgtttac tgaaagagaa tttttatcct gtaatggctg cgattttagc
      121 aggaatagtc gcaaattgct tggttagtgt agggttcggt tacttcttct ctcttcataa
      181 agagatggtg atctcattac tacctaaatc tgtaacgacg gctatttctg tcgatttatc
      241 tcatacgatg ggcggggtga atgccgtgac acttgctatt gttgtctcta caggtatttt
      301 tggttcgctg atcgccactc atattttccg cctattcaag atcgaaagtc cagttgctcg
      361 cggtgtggcg ttaggaagta cttctcatgc tattggtaca gcaaaagcaa tcgagattgg
      421 tgaaatagaa ggaatcattt ccggacttgc gatatgcgtc aacggaatat tgaccgtttt
      481 attgctaccg ttattctttc agccttttgc tggcttgttt tgatgcgaaa accactattt
      541 tatgctacaa taaccaaaac gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct
      601 tccgagtatc ttaaacaatt tgataaagat ctatattata agattctcct cctagaaagt
      661 tttgaggatc aagcttggca taccgctgca caattagcgc aagttgttca gctggatgcc
      721 cgcagcgtgt cgaaatacct gaacgagctg tctaaaaact atcagcagtt tagcggtaag
      781 acacaccctt tgtttacaaa aaatcaccgt tctggttata acttttatga tacgctcgat
      841 tcaattgaac atgagcgttt tttgatctat ctcgtgcaaa gtacgctgaa attccagttg
      901 ctgcacgaca ttttttttga agaattccat acaatgtacc aatttgcgca aaagcattac
      961 attagtgaat ctactgctca tcgaaaaatc aatgaatgga aacagcaact gcaaacttac
     1021 ggaattcggc tgcaacgagg tacatatatc gctcaaggcg aagaagaaat cattcgactt
     1081 tacttgcata tgacattctg gcagttgttt cgagggaaaa tctggccatt tgaaacgatt
     1141 tctcaaatgg acgtaaagaa tatggcagaa catatcatgg ctttttttaa tgttcgtctg
     1201 aacgagatca aaaaaagacg attggaatat atgctaggtg cctttttttt aagaaaatca
     1261 cagaagcatt atgttgtatt gaatgagaaa aaaagacggc tgatttctga caatctgttg
     1321 ttccagcgat tttgccaggt catggagccg gtatttccta attattttca agtagaagac
     1381 gagttaggcg cattatttct tgtactgatg actcgtgaag aatattatag tgatcctaag
     1441 atacggaaga aaatttttga ttttcatcaa caagcaaaaa caccgccctt tacagccctt
     1501 tcagaagcaa aagccgctct ttctctttat caggaagagc aggggctgcc agcagaaaat
     1561 ctaaccttcg aggcagaaaa ctatttattc agcagtcatt ttttcgccta tctttttcct
     1621 aatgcgaaag aaacgattga tggcaatagt agtgatttta ttaatcattt agttattgaa
     1681 aacaaggaat tgaaacaatg gctcgtacat ttttttgaaa gtagacacaa acatcccaat
     1741 catctggcat ttaaaaatca tgcgtttttg atggggcggt atctgactgt gtttaaaaca
     1801 ttaggagcct ttactccaca gcttccaaag atcacgatcc ttttaatgac agatttccca
     1861 ttatttgaag agcaactgtt agaggaggga ttacggacat ttttccgtaa tgagtatcag
     1921 ctgatttttc tgccaacaga ttatcggggg agagaagttg atcttctgat ttcgacaagt
     1981 aaagtccacc gcaaaccatg ggcggatctt gactatttta ttgtgactga agaactaaaa
     2041 ttaattgatt acatccaact atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta
     2101 aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc agaattgaaa
     2161 aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt tgttctcgta
     2221 tccgatgatg aatttgatgg ctgtccggtg acgccgtact tattgatcaa aacaagtgac
     2281 gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga ttgggcgatc
     2341 gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc acaggagctg
     2401 cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct tttgtatttg
     2461 gattagatca aaagttcggt aaaatggaag aaatttcttt gcgatttcct gaaacttttg
     2521 cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta tcgaaatgcc
     2581 aaaatcattt tggtgaagtt acgattttac tgaatacagc ggggatattg gatgcctaca
     2641 aacccttgat ggaaactgac gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa
     2701 gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccaaaaa agcggaacga
     2761 tcatcaatat ggcttcgatt gctggcttga ttgctggtgg aggtgggatc gcttatacaa
     2821 gcgcgaaaca tgcgattgtc ggatttacca aacaactcgc tctggattac gcaggacagg
     2881 gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat gcggctgatt
     2941 ttgaagggaa aggtgagatg gcagagtggg tggcaaatga aacaccagtc aagcgatggg
     3001 cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa gcaagctaca
     3061 tacaaggaac gatcatccca atcgacggcg gatggttgtt aaaataacct tgtgtttttt
     3121 tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa aaaaagtcga
     3181 tgactgttcc cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt
     3241 taatattcga aactaagcgg cttttcacga cctcttgata taaacgatat tcaaaagctt
     3301 gcaactgcgg taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacca
     3361 ttcttattac ttgttgcagg gcagcttttt cactaccttc tgcgacactt cagttggaat
     3421 tttcttccca tcctttggtc gcggtcatta ccaagataaa attatcttgc aaaggagaaa
     3481 tagaacatgg aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact
     3541 cggatggcgc tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc
     3601 tttggagtga ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa
     3661 agatacatcg tcgcggtaac agcgggagta gctttggcta atattgcttc tcctcttgga
     3721 ctgatagatg ttgcagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa
     3781 atcactagtc gcatcaaaaa catgaaaatc aaaatggccg tgacagcatg cgtatttgct
     3841 ttttccatgt ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg
     3901 aattggtggg tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt
     3961 tattggatcg gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaataggg
     4021 cagcatggaa gagactgaat gttgagtctt cttccatgct gcttttttga ttaatttgta
     4081 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     4141 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     4201 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     4261 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     4321 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     4381 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     4441 ccaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     4501 gtttgccctg tcaaaggaac caaaccaaaa ggaatggtca tctgagaaag gacagaaata
     4561 atcgcggcaa attccgcagc aagtatcaat tctttcaatg ttcggttcat aacgccctct
     4621 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     4681 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     4741 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     4801 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     4861 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     4921 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     4981 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     5041 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     5101 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     5161 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     5221 gtattctgat tgtttgccac ggctattagg tccatgttga cctggtgcgt aagggcgacg
     5281 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     5341 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     5401 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     5461 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     5521 ctgcattatt ttacacaaat tacagtatat gatagatcat ccacgaatgt caagataatt
     5581 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gtatgtgcaa
     5641 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     5701 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     5761 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     5821 gcgatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     5881 atgttcagct ggggtctggt catgattcta tacatgaacg ccgaattagg aacatcgaat
     5941 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     6001 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttctaatt
     6061 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     6121 gtaaaactag caaaaccttc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     6181 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca
     6241 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     6301 cctgccttta cgcttactta ctttgcttct cacggacaga tggatggaat gacagtcagc
     6361 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     6421 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     6481 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     6541 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     6601 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     6661 ttagcttttg ttcattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     6721 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     6781 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     6841 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     6901 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     6961 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     7021 aaagcaattg gttgggcatt acgcgattat agtaagaccg atgccgattg ggtaaaaaga
     7081 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     7141 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     7201 agattcaaac aaacaagctt tagatagcac aagagaagca ttggaaaaat tacgcaaagc
     7261 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     7321 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     7381 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     7441 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     7501 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     7561 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     7621 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     7681 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     7741 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     7801 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     7861 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     7921 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     7981 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagctttcg
     8041 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     8101 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     8161 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     8221 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     8281 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
     8341 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
     8401 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
     8461 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
     8521 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
     8581 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
     8641 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
     8701 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
     8761 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
     8821 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
     8881 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
     8941 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gactgcatta
     9001 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
     9061 ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa cgatgatcga
     9121 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
     9181 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
     9241 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
     9301 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa aagaagcaat
     9361 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
     9421 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
     9481 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
     9541 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
     9601 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
     9661 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
     9721 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
     9781 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
     9841 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
     9901 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
     9961 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    10021 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    10081 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    10141 ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa ctattgactt
    10201 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    10261 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    10321 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    10381 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    10441 aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt atcgatttgc
    10501 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    10561 atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg ccaatgatca
    10621 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    10681 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    10741 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    10801 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    10861 gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaattacaa
    10921 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    10981 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    11041 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    11101 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    11161 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    11221 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    11281 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    11341 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    11401 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    11461 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    11521 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    11581 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    11641 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    11701 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    11761 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    11821 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    11881 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    11941 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    12001 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    12061 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    12121 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    12181 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    12241 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    12301 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    12361 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    12421 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    12481 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    12541 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    12601 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    12661 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    12721 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    12781 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    12841 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    12901 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    12961 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    13021 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    13081 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    13141 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    13201 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    13261 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    13321 cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    13381 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    13441 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    13501 aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac aagcagataa
    13561 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    13621 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    13681 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    13741 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    13801 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    13861 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    13921 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    13981 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    14041 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    14101 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    14161 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    14221 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    14281 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    14341 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    14401 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    14461 aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    14521 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    14581 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    14641 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    14701 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    14761 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    14821 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    14881 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    14941 actctgacta agattttact tcagctttac cgactgcttc gcccgggcga acaagtcctg
    15001 attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca ataatgtcag
    15061 ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg gtctctggtg
    15121 tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt tcaacagtat
    15181 ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca atggcatgtt
    15241 ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg ccgtttgacg
    15301 gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca tttacttcag
    15361 ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt gcaactgccg
    15421 taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt
    15481 gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc aaaattccct
    15541 gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc attgcgatga
    15601 tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg cctactccga
    15661 tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca gagccgacca
    15721 ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca ggttcagtta
    15781 cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt ttattcccac
    15841 gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat aaagcaatca
    15901 tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg gcattggtca
    15961 tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg gcactaaaca
    16021 accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag cttccgactg
    16081 tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac aatgaaaaca
    16141 atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag atttctagat
    16201 acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa ccaatcatct
    16261 ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg gaagctacac
    16321 tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt cctaatattt
    16381 gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga aaatggaaaa
    16441 ttgcttcacc cggtaaccac aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa
    16501 cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg aatccaagga
    16561 tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa
    16621 tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt tctttggaaa
    16681 ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca tcgtttccta
    16741 tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt atatcttcga
    16801 tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga gtcgcacaat
    16861 gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc tcggcatctg
    16921 cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc aagttgtttc
    16981 ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa agtacgagag
    17041 aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg aacattgaaa
    17101 tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat
    17161 gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga aagaatatcc
    17221 tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg tttcacgaga
    17281 aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa aaaaacaagg
    17341 aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg gattgactag
    17401 ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc atacgttaga
    17461 agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg gtgcgcctgt
    17521 ttggcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag acaccgatta
    17581 tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat cgatctatga
    17641 atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga tcacagtaga
    17701 agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt atgcagtagt
    17761 agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg agtcggttca
    17821 ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca
    17881 aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc acaaaggggg
    17941 catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa aagttatcac
    18001 ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc gggaaatatg
    18061 atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg ttattcagct
    18121 ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca ataacgaaaa
    18181 gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa gttcgaagca
    18241 aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat caaggtggtc
    18301 tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag atggatgaat
    18361 taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag agcggtacaa
    18421 tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta tttgaaagaa
    18481 acgctcccta gactcctttt ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt
    18541 gtgcaaaaga aagaggacta gaatatgtat gaatatctga caggtgtggt tacctttatc
    18601 aacccctatt acatagtagt agaaaccaat ggtatcggct accagatcgc actaggaaat
    18661 ccttaccgct acagtagcaa actaaatcaa aaaataaaaa tttatgtcca tcaggtaata
    18721 cgtgaagatg cgcatcttct gtatggattt gattcgctag aagaaaagca actgtttttg
    18781 cgattagtaa gtgtttcagg aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa
    18841 gaccactccg gtttgattca agcagtagaa tccagtgatg tcacttattt gacgaaattt
    18901 ccaggagtag ggaaaaagac agcacaacaa atgatcttgg atctaaaagg aaaattcggt
    18961 gagctgagta tcgatacacc ttttagtcta tttgatgaag caaacacaaa agacgctaca
    19021 gctctatctg aagcaatgga agctctctcg gctcttggtt acagtgatcg agaaatcaaa
    19081 cgtgtagaaa aacaattaaa agaagtagat aatcagacaa cggatgagta tctacgccaa
    19141 gcactcaagc taatgatgaa aaaataagga ggagaccaaa tgacagatga atacttgtta
    19201 tcaccagaat ctggcgaaaa tgaactgagt ttggaaaaat ctcttcgacc acagctgctt
    19261 tctcaatata tcggacagag aaaagtcaaa caagaacttc agatttacat cgaagctgcc
    19321 agaaatcggg aagaaccttt agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa
    19381 acgacgatgg ccatggtcat tgctaatgag atgaccgtaa atattcgtac gacgagtggc
    19441 cctgctatcg aacgtccggg tgacttagta gcaatcttga atgaactgga agcaggggat
    19501 gtcctgttta tcgatgagat ccatcgatta cctagagtgg tagaagagat gctttattca
    19561 gctatggaag atttctatgt agatatcatg gtaggacaag ggccaaccgc acatccggtt
    19621 catttccctc ttcctccctt tactttgatt ggcgcgacga cacgggcagg tatgctttct
    19681 gcacctttac gagatcgatt tggcatcatt tctcatatgg aatattatga cgaacaggat
    19741 ctgcaggaga tcgttttacg ttcttcggat atttttcaaa cagagatcat tgaagaaggg
    19801 gcattggaaa ttgctcgccg atcacgagga acgcctagaa tcgctaatcg cttgctcaaa
    19861 cgggttcgtg attttgcgca agtccaagga gacgggaaaa tcgatcgtcc tatagcggat
    19921 caggcactta ctttgctaca ggtcgatcag gctggattgg attatgttga ccagaaattg
    19981 ttgcgaacaa tgatcgagtt atatggcggc ggaccagtag ggttaagtac gctttcagta
    20041 aacataggag aagagcgtga gacggtagaa gatatgtatg aaccgtatct gatccaaaaa
    20101 ggatttttaa agcgtacacc tagagggcga attgctactg cttatgccta tgaacatttt
    20161 ggttacgact atcatatatg atttttgctt ttagattgga ggaaaatgaa tgagtgatac
    20221 aacacgtacg aaacaattga tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt
    20281 tcgaaaaatc agcgtccaaa aaatcgcaaa cgaagcaaaa atcaatcgac aaacatttta
    20341 ttatcatttt acagataaat acgatttgct tcgttgggtc tattgtgagg atgcattgca
    20401 ttatttagat ccacaatgtc tttcacttga taattgggaa gagcaagcat tgaaaatgct
    20461 gaaagcaatt gctgagaaga aagaatttta tgcaaatact gtgctttctg acccagaaat
    20521 tttacaaaaa gagttttctc aactgatcca aaaatcattt atcaagattt ttgaacaaat
    20581 ggatgaagaa agacaacttt ctgcaagaga caaactattt tatgctcgtt ttttttctta
    20641 cggttgcagt ggtgtattag tcaattggat cacgaatgaa tatgaagaaa caccgatgga
    20701 aatcgctatg cagcttttcc gtttagccaa ggatacggaa ttatattctt atcgtcttta
    20761 tgcaaaagaa gagttggaag aacagtaata gaaaaatttt ttgttggaaa agcaataatc
    20821 gctaaaagga gatggggaaa tgacaaaaat tttatttgtt tgtttgggaa atatttgccg
    20881 ttcaccgatg gcagaaggct tgctgagaaa acgaatagca ttagaaggaa gggaagcaga
    20941 attttttgtg gattctgctg ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg
    21001 aacgaaaaaa atattaaacc aagaaaaagt agatatgacg aatatggttg ctcggcaaat
    21061 cacaccccac gattttgaaa cttttgattg gatcattggc atggatcaag aaaatgtaga
    21121 agaactgaaa cgaagagcac cacgctcagc gcaggggaaa atccatttgt ttttaagtag
    21181 cattcctgga aaagaaaaag aaaatgttcc cgacccatac tataccaata actttgatga
    21241 gacatatcac ttgatcacag aaggcctgaa tcattggtgg aatatttgga ttgatgaaaa
    21301 taaaataaca aacaatttgt aaagttaacg ctttcttttt aaggcgatgt tagagcattt
    21361 ccttgttttt ttgtaaaatg ttaggtaaaa taaaattaat taagtaacac tttcttagct
    21421 aaaggaggag tgcattcgat gaacgaagag acagtatcgc caatgaagaa gcttacgaag
    21481 ttagttactc atacttcgta tcaacttttt gattcttttg taaatgacac tgaagaacat
    21541 gctaagaatg tacgattgca aggaaagttt attcttgagc tgcaaaaagc tgctactgaa
    21601 catagtctag ttatcttaca agtaagagca aatcgtttga gtagcaagta tgaaacgatc
    21661 tcaggctggg tggcgacaaa aacggtcgaa aaagaccgag tgatgatccg cctacaaaac
    21721 gaagagcagc agatccggat ggttcctgta gaacaaattg aaaaaataac aatcctttca
    21781 ccagcagggg aacaggaacg gttatcaaaa taatttatac cagatagaaa agcacgtatg
    21841 agcttgtacc atcaatcatt actaatcaaa aataaccgaa tgctttaagc tgatatcagt
    21901 aaaacggaac gttttaggat gagtatcata gcatgacagc attcggttga ttagttgaat
    21961 cgattggatg gtacaagttt ttttgataaa aaaaggtcct gctagccggg gagctagcag
    22021 gataaggagt taaaaatgaa aaagtgttgt tagggttgtt tgttggtatg cttatataat
    22081 agtcattagt tgtgaatatt ttgtgacgaa agttttcgaa agttgttaag tt
//
LOCUS       DACCCM010000048        21503 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCCM010000048 DACCCM010000000
VERSION     DACCCM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.050"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            5..403
                     /locus_tag="IVK78_001765"
     CDS             5..403
                     /locus_tag="IVK78_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6375238.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IVK78_001766"
     CDS             387..1082
                     /locus_tag="IVK78_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP6375239.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IVK78_001767"
     CDS             complement(1666..2553)
                     /locus_tag="IVK78_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP6375240.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IVK78_001768"
     CDS             2738..3577
                     /locus_tag="IVK78_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6375241.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="IVK78_001769"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="IVK78_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP6375242.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="IVK78_001770"
     CDS             5045..5401
                     /locus_tag="IVK78_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375243.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="IVK78_001771"
     CDS             5404..6561
                     /locus_tag="IVK78_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375244.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="IVK78_001772"
     CDS             complement(6587..7027)
                     /locus_tag="IVK78_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6375245.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="IVK78_001773"
     CDS             7187..7693
                     /locus_tag="IVK78_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6375246.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="IVK78_001774"
     CDS             7861..9774
                     /locus_tag="IVK78_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6375247.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="IVK78_001775"
     CDS             9779..10780
                     /locus_tag="IVK78_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6375248.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="IVK78_001776"
     CDS             complement(10982..11188)
                     /locus_tag="IVK78_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP6375249.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="IVK78_001777"
     CDS             complement(11834..12682)
                     /locus_tag="IVK78_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6375250.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12846..13748
                     /gene="gnd"
                     /locus_tag="IVK78_001778"
     CDS             12846..13748
                     /gene="gnd"
                     /locus_tag="IVK78_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP6375251.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="IVK78_001779"
     CDS             13750..14751
                     /locus_tag="IVK78_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6375252.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="IVK78_001780"
     CDS             14748..15032
                     /locus_tag="IVK78_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6375253.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="IVK78_001781"
     CDS             15065..15862
                     /locus_tag="IVK78_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6375254.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="IVK78_001782"
     CDS             15876..16709
                     /locus_tag="IVK78_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6375255.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="IVK78_001783"
     CDS             16727..17584
                     /locus_tag="IVK78_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6375256.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="IVK78_001784"
     CDS             17606..18115
                     /locus_tag="IVK78_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6375257.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="IVK78_001785"
     CDS             18152..18553
                     /locus_tag="IVK78_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375258.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="IVK78_001786"
     CDS             18665..19018
                     /locus_tag="IVK78_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375259.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="IVK78_001787"
     CDS             19052..19495
                     /locus_tag="IVK78_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6375260.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="IVK78_001788"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="IVK78_001788"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6375261.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVALPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="IVK78_001789"
     CDS             20590..21303
                     /locus_tag="IVK78_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6375262.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7398 a         3302 c         4491 g         6312 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactgc ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctttgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttc
//
LOCUS       DACCCM010000049        21263 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCCM010000049 DACCCM010000000
VERSION     DACCCM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.051"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            87..1079
                     /locus_tag="IVK78_001790"
     CDS             87..1079
                     /locus_tag="IVK78_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6375263.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1139..2560
                     /locus_tag="IVK78_001791"
     CDS             1139..2560
                     /locus_tag="IVK78_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP6375264.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2553..4052
                     /locus_tag="IVK78_001792"
     CDS             2553..4052
                     /locus_tag="IVK78_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP6375265.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4091..5485
                     /gene="uxaC"
                     /locus_tag="IVK78_001793"
     CDS             4091..5485
                     /gene="uxaC"
                     /locus_tag="IVK78_001793"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6375266.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5644..6327
                     /locus_tag="IVK78_001794"
     CDS             5644..6327
                     /locus_tag="IVK78_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP6375267.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6329..7165
                     /locus_tag="IVK78_001795"
     CDS             6329..7165
                     /locus_tag="IVK78_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6375268.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7176..8930
                     /locus_tag="IVK78_001796"
     CDS             7176..8930
                     /locus_tag="IVK78_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375269.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9206..9547)
                     /locus_tag="IVK78_001797"
     CDS             complement(9206..9547)
                     /locus_tag="IVK78_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6375270.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9615..11786)
                     /locus_tag="IVK78_001798"
     CDS             complement(9615..11786)
                     /locus_tag="IVK78_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6375271.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            11997..12854
                     /locus_tag="IVK78_001799"
     CDS             11997..12854
                     /locus_tag="IVK78_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6375272.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(12925..13782)
                     /locus_tag="IVK78_001800"
     CDS             complement(12925..13782)
                     /locus_tag="IVK78_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6375273.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(13767..16469)
                     /locus_tag="IVK78_001801"
     CDS             complement(13767..16469)
                     /locus_tag="IVK78_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6375274.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(16482..17771)
                     /locus_tag="IVK78_001802"
     CDS             complement(16482..17771)
                     /locus_tag="IVK78_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP6375275.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17921..18967
                     /locus_tag="IVK78_001803"
     CDS             17921..18967
                     /locus_tag="IVK78_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6375276.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            18969..21122
                     /locus_tag="IVK78_001804"
     CDS             18969..21122
                     /locus_tag="IVK78_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6375277.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         6279 a         4318 c         4042 g         6624 t
ORIGIN      
        1 catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa
       61 agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg
      121 gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa
      181 caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa
      241 aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag
      301 gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa
      361 attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac
      421 gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc
      481 tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta
      541 tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat
      601 gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac
      661 gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt
      721 ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac
      781 tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga
      841 gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg
      901 attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag
      961 aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac
     1021 aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa
     1081 ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat
     1141 gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt
     1201 tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca
     1261 acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat
     1321 gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat
     1381 cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg
     1441 ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac
     1501 agaggcagga atccaattcg tggagactga taatttaaca gatactcccc cacagagcct
     1561 cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac
     1621 catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg
     1681 ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt
     1741 tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt
     1801 tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct
     1861 ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt
     1921 gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa
     1981 tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga
     2041 agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat
     2101 tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg
     2161 tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa
     2221 gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc
     2281 accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa
     2341 gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac
     2401 agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc
     2461 gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc
     2521 agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga
     2581 aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac
     2641 tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg
     2701 cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg
     2761 tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt
     2821 ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga
     2881 accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt
     2941 ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca
     3001 aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag catccttatg
     3061 gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa
     3121 atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc
     3181 ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc
     3241 aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg
     3301 cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg
     3361 gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact
     3421 acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag
     3481 aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga
     3541 tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc
     3601 ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa
     3661 aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag
     3721 ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat
     3781 ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc
     3841 caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt
     3901 ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa
     3961 aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa
     4021 ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga
     4081 aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct
     4141 ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa
     4201 agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca
     4261 ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc
     4321 ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc
     4381 gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa
     4441 tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt
     4501 tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc
     4561 cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct
     4621 accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt
     4681 cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt
     4741 cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt
     4801 agacatctta cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc
     4861 acgagctaac aaactattat cttcagatga aatcgatgct tatcgtacag aaacactgat
     4921 acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta
     4981 tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat
     5041 gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga
     5101 tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc
     5161 tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg
     5221 gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc
     5281 tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca
     5341 tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc
     5401 acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa
     5461 aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg
     5521 atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga ccaaatttgc
     5581 tccgtcttct tctcttgctg ttctgcttca ttgcctataa acctttatga aagaagagat
     5641 aacatgccta ctatccatat tgccggtgat tctactgcgg cagaaaaaga tgaaaacaag
     5701 cgtcccgaga caggttgggg agaaaaaatc tctgcttatt tttcacccga gatccagatc
     5761 aataaccaag caaagaatgg acgcagcacc aaatcattta tccaggaagg aaggttagcc
     5821 cgtctagctt cacagtttca gccgaatgat ttcttatttg tacaatttgg acataacgac
     5881 cagaaaatcc aagaagaaag aggtacgctt cccttcggta gctatttaaa aaatctgcag
     5941 atattttttg aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac atctgtgacg
     6001 cgacgagatt acttagaaaa cggaacattg aatccagata tacttggaga ctatccaaaa
     6061 gcgatgcgtg catttgctga gaaacaccat attcctcttt tagatgtgtt ctcaagatca
     6121 caagaattgt tccagacctt ctcaaaagaa gagacaaaaa aattctacct gcatcttata
     6181 cctgatactt gtaaaaatta cccagaagga ctaaaagaca atacccactt tagtccagaa
     6241 ggcgctgata aagttgcccg aataattgtt gaattgataa aagaatcccg tctgcctctt
     6301 gctaattatt tacaaaaagg agtttgagat gtttccatta aatttaggca tccgtgccca
     6361 cgatttagat gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc ttcacttgtc
     6421 ccatatccaa tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa ctgacatgac
     6481 acttgggagt gctgcctatt ttggtgaata cttccaaaaa aacaatatcg aactttccat
     6541 tttaggatgc tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac aagcattggc
     6601 aactttcaaa cagcaaattg ctctgtgctc tgcctatcaa gccaagatga ttgctacgga
     6661 aaccggtagt gtctctgtcg gttacacaaa agaaaatttt actgaagaag cttatcagat
     6721 cgccagaaat tctatcattg aattagtcgc tcatgcagaa aattttggcg taactgttgc
     6781 tgttgaagca gggatcaatc atccaatcta taactatcag ttagctaaac gtttaatcga
     6841 tgaagtagct tcaccaaatt taaaaatcat tttagattgt gcgaatttga tgcataaaga
     6901 aaatcatgct gatcaggaaa aagtgatccg aggtgccttg gacaatttac atggttatat
     6961 tacagcgctt catctcaaag actatatcat ggaaaatgga aagatccgca tggtacctgt
     7021 tggcaagggc tgtttagatt tccgcccggt actgcattat atcaaatatg agcgtcctct
     7081 aatgtacgcc actcttgaag ctactcctga gatttatgtt cctgaagcaa tagaacactt
     7141 gcagtttatt taccaagatt gttaaggaga agattatgga ggaacgaata cagaaaagtt
     7201 atcaaaaata tcatgcaaat gcttttcaga cattgctgca tctttatcag ggaaattatt
     7261 ggcgttttgc catgtcagga ctctttttta ttatcaaaca tcttccatct tggttaatgc
     7321 cgatcgctat tgccaatgta atcaatgcag cttcatcgaa agattcttct cagttgcagg
     7381 ccatttattt caacgctttt ttgatggctt gtctgattgg tcaaaatata ttgacgaatt
     7441 attttcatgt aaaataccat agcacttcga tccgtcaagt agaagctgga ctaagagcga
     7501 cccttatcaa gaaaatccaa gaactatcta ttccttatca aaaagaattg caatctggac
     7561 gaatccagtc aaagatcatc cgtgatgtag aagctatcca aacgttgtcc tctcaagtat
     7621 ttgtcagcgg attaaatatc gctgtcaatt tattcgtagc tttaggtatc acgctttata
     7681 aaagtcctgt tgttttcatg tttttcttat tgactgttcc tctcgccgca ttcgttactc
     7741 tctttttccg taaaaggatc agacagtcca actcagactt caggcaagaa atggaagaga
     7801 cttctgcaaa agtgatcgaa gtgatcgaat tagcaccgat tgctcgagct catgggttag
     7861 aaaaacacga aacaactcgc ttaaataagc attttactca aatctatgaa aaaggattcc
     7921 acttagatat cctgcaatct gttttcggat cagtcagctg ggctagtttt cagatatttc
     7981 agctgatttg tttgattttc actgggatat tagctttaca agggcgcatc caacccggcg
     8041 atgtcgtgat gtatcagacg tatttcacga cgatcgtcaa ttccgtttct ggaatgatca
     8101 cgctgattcc tacgatttca aaaggcatgg aatctatcac ttctattggc gaactgttga
     8161 ttgctggcga tgtagaaact tatcacggta agaaaaaaat ccaacatttg gatggaaatt
     8221 ttcgttttga ggatgtttct tttctttatc cggactcact caaaccgatt atcgatcatt
     8281 tttctctaga ggtgaaagct ggagaaacaa ttgcttttgt tgggccatct ggctctggaa
     8341 aatctacact ccttaactta ttgattggat ttatccagcc gacttcagga aaaatttttc
     8401 tagatgatca gcctttggat gaactggata tgcgaagtgt gcgaaattat ttagccgttg
     8461 ttcctcaaac cactttgttg ttctccgctt cgatcaaaga gaacatcact tatggcttga
     8521 aaaacgtatc aaaagaacgt ttagatgaag tcatcgaagc tgcgcaactt tcttcactga
     8581 ttgaccagct tcctgatggt ctggatacct tagtaggcga acatggcaac aagctgtctg
     8641 gtggccaaaa gcaacgtatc tccattgcac gagcgttgat acgtcagcca aaaatcattt
     8701 tgctagatga agcaacctca gctttagata accagtcaga gaaaaaaatc cagcaagccc
     8761 tgaattacct gacagaaaca cccactactt ttattgtggc tcatcggctt tcaacgatca
     8821 aagaggcaga taaaatcgtt gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg
     8881 aaaaactgtt agctcaaaaa ggctatttct accgcttgaa aaatgaatga caaaaaatag
     8941 gagctatgac aagacttttg tcgtagctcc tatttctgaa taaacggtgt tctcctgcca
     9001 actcctcggt cacaagtcac aatagtcatc aaacatgaga agctgttttt tgaaattgtt
     9061 ccttgtaact cagagttaaa cggtaggctc acgacctctt ggtaaatggt gttcaagaga
     9121 cagcctatcg gcaataagaa gcaattttgt catcgctcaa ggccgaacgt ctctttcaca
     9181 ccctctgttg tttcataaaa tagttttatt cgctgtaaag gtcgcgaact ggtgtcatga
     9241 tgatacggtt caagtcattg atgattgtac tgaagccttg ctctttttgc atcaagtcat
     9301 tgattacttc ttctgattgg attttcacag ccatttcttg tgctttcatt gcatcttcgt
     9361 cgctgaactc ttgtccactc atttgttttt cttgtaattc gatttgcatc gcttggaagt
     9421 ctttgaacag tttgtgtgct tcttcgtttg cttgtacttt ctcgtacgct tctttcaacg
     9481 ctttgaattc tggtaattca cggatctcac gttcgatttg attcgcgcta tcgtagatgt
     9541 ttgacatgtt atgccctctt ttcttttagt agttactctc tattgtaaca tggggttatt
     9601 cgttttcaaa ggggctactc tccttgatat ttccgccaga ggtctttagc cgcttcacca
     9661 attccctgaa cgccttcttt caccatatcg ccgaagtttt tcagcccttc ttttgcttgt
     9721 tcaccatatt gattcaagtt atcttgccaa tttccagact ctccattaga atttcctgtg
     9781 tttccaactt gatctgcagc taccacttgt cctcctgtgg cataggcatc tgctacacta
     9841 aatcctgttt ttgaagtata cggaaggatg ccttctgccg ctgatttgaa gactttcccg
     9901 acaacatttc cacttgtccc ttccaaatag tgcagcttgc tggattcttc gtaacccatc
     9961 catgtcgaaa tcactacgtc tggcgtgtaa ccgatcatcc attggtcatt tgcttttgtg
    10021 gcatcgaaat tagtttctgt ggctcctgtt ttccctgcta tcgtataacc tgcaggattc
    10081 gcagcagccg ctgttccatt tgaaaatgta ccaagaagca tgctggtcat ttcatcggct
    10141 gtttcctttg atatgacctg ttttggtttt ttgtttgtgt tatcgacgat caccgctcca
    10201 gtcgaatcaa cgattttcgt gataagatgc ggcgtataca tctgaccatc attggcaaat
    10261 gcactgtaag cacccgccat gatcaatgga gatactcctt tttccagtcc accaagggct
    10321 aagccataat acttatcaga ttttgctaaa ggcaggccga attcttctgc tttatcatat
    10381 cctttttgta aaccgatctc atgcaaaagc catacagtag gcaaattcaa actttgtgca
    10441 acagcttgat acatcggtac ttctccttgg tatgtcccgt caaaattatg cgcatcgtaa
    10501 taactttgcg gttcgtcttt caagatactg gaaggcttgt atccagcttc cagtgctggt
    10561 gtatatacgc ttaatggttt gatcgttgat cctggtgaac gacgcatctg tgtagcaaag
    10621 ctgaatcctc taaagacatg atctcctctt cgtcctacta gtgcttgtac accacctgta
    10681 ttcggatcta aagctactga agcagattgg accatggcac catctgaagc atttgctggg
    10741 aataatgcac tgttttgata agtagcttct aattgctttt gataatcctg atctaaagct
    10801 gtatagattt tatagccttt attcatgaca tcttcttcat caagaccata ctcgttaact
    10861 gcttcatcga tcactgcatc aaaataataa ggataacgat aaccagaatc tgaattatcg
    10921 tatgtgtcat taagatacgc gctgatatct gtctggcttt ctgcttgttc ttgttctttt
    10981 gtgatttttc cgttttcttc cattacactt agtactgtgt ttttccgatc attcgcattt
    11041 tttgggtgat cgatcggatt ataaatacca ggacctttca gcattcctgc caaagtagca
    11101 gcctcagaca gcgtaacatt cgttgcatct actccaaaat actttctgga agcgtcttgg
    11161 acaccccata ccccgttgcc aaaataggca ttgttaagat acatggtcaa gatttcttct
    11221 ttgctgtact tcttttcgat ctcgattgcc aaaaataatt ccttcgcttt tcgatcaaac
    11281 gtttggtcta atgtcagata cgcatttttg gcaagctgct gagtgatcgt acttcctccg
    11341 ccccctgatg ttccgccaga caagaccatt cggactgctg cacgggcaat ccctttgata
    11401 tcaaacccgt gatgctcgta aaaattccta tcttctgtcg agattactgc atcttggata
    11461 tagggagaaa tcgcgtttaa ttctacatat gttccttttt gggcgtacag tgtgccagct
    11521 tcttcgtttt tcttatcata aatgactgtc gattggctta atcctgattt taacgtttcg
    11581 acatttgctt gttttgctaa aataaagagg tagatacttg tcactaagac acagatcaaa
    11641 ccaatcagca aaatcaattt gttcacatga tattttttcc agatgcgctt tcgccatcga
    11701 tggaattgtc cgagatatgg tctgatccaa cgccaaaaac tgatcaaagc agcttttatc
    11761 ttttctaaaa tgctttgaaa atccatttgc ttcttctcct gtctatttga ttcaatcgtc
    11821 ttattttacc atagtgaaag agtgttttct tcctgcttaa gttgtagttc aatatgtttt
    11881 tttacgttag tgcaagtaga gtaacataag aatgaacaga aaaatgtaag ttttttctta
    11941 aatatgtttt aaaaatggta caattagtct gcgattgcac tgaaaggagt acacttgtga
    12001 aaatcacgat cactttacct atgaaccatc ctgaaacaac aattcgcgac ttgcttgaaa
    12061 aggaatggtt agttcctaga aaggttcgac actttttgcg tatacgtaaa aacgtttgga
    12121 tcaatcaaca acctgctttg ttccatcatg aagtaaaggg caatgaccag atcacattgt
    12181 tattcgaaga aacagattat aattatcaag aagttcaatt aggagatgcg aagcacatcc
    12241 gtgttttata tgaagatgag catttgatta tagtggataa gcctgcagga atgaagactc
    12301 atccaaatga accgacagaa aacagtacct tactcaatca tttagctgct tatctcgaca
    12361 ctaaacagca acgaccttac gttgtccacc gcttggacaa agaaacaagc ggtgccgttt
    12421 tatttgcaaa aaatccattt gtcttgccga tacttggaag aatgttggaa caaaaaatga
    12481 tctatcggag atatcaagcc atcgtatggg gagaaattgc caaagatatg accatcacca
    12541 ataaaattgg acgagatcgc catgatcgga gaaaaagggt cgtagatgat aaaaatggtc
    12601 agagcgcctt gacacatgtc actgtcaaca aagtactttc tgaacaaaca caagtctatt
    12661 gtgttttgga aaccggacgt acacaccaga tcagagttca tttagctcat aagggacatc
    12721 cgatcgttgg agaccctctc taccaaaata gaacagctga acgactgatg ctccatgcat
    12781 tagaactgca catgatccat ccatttactc aagaaaaaat agtcggtaaa gctttacctg
    12841 gattatggga atagaaataa aaaagaacag agtcttatca gcagataatg ataagactct
    12901 gttctttttg tgtaatttgt ctgcttactt ttgatgttcg tgttctgcta acagcaaact
    12961 accgataatc ccactttcat tcggcagtcc ccaacgaacg atatattcac ttacttgagg
    13021 tgtttccata taaccaccca tcaattcaac aaattgctga cggatttttt gaaggagatg
    13081 atcctgattc attaccccac cgcctaaaat aatcttttca ggtgctaacg tcaacgtata
    13141 gttgatcagt gcttgagcaa tataataagc ctgaatagcc caaacaggat gatcttgcgg
    13201 cagatcttgc cctttgatcc ctgtgcgtgc ctcaatagac ggtcctgctg ctaatccttc
    13261 taaacaatct ttatgataag gacaagtacc ttcataatgg tcatctggat ggcgtttcac
    13321 ccaaatatgt cccatttcag gatgtgcgat ccctgaaaaa atttcttcat tgatcacgac
    13381 acctgcaccg attcctgttc ccacggtaag gtaaatacaa ctattttttc cttgtgctgc
    13441 tccttgcttc aattccccat atgcagcagc attgacatcc gttgtccaag cagcaggaat
    13501 attgtagcgt tccttgattg tgccaagaaa atcgaattgt gcccaccctt ttttgggagt
    13561 tgccaaaacg tatccgtaat tatcttttgt tttgtcgata ccaatcgggc cgaatgagcc
    13621 aatcccgatt cccttcaatt cataacgatc aaaaaattca aatacctgat ccaatgtttc
    13681 ttctggtgtt gtggtaggga tacttaattt ttctaaaatt tctccaccat ctgaaaccgc
    13741 acacacaaat tttgttccgc ctgcttctat tccgccatac attcgttttc ctcctcgaat
    13801 ccgattgtcc gaatctcata aggttggatt attgggacaa ccgcttgctt cgttggttct
    13861 tcaagcagat tcaatatgac agtttttcca ttgtctttag ttatttccaa tttttctaaa
    13921 tgactagaca tattgaaacc tcttactact actttatcac taaacttgct gcgtttcaaa
    13981 gctgtaattg caaacgtttc actttttatc gttaaatatt gttgttttga aatcaatgtt
    14041 ccttcatgat gtttgatttg ttgagtggaa aaaggaatct gtgctgcgta tgcatgcaaa
    14101 taagtagaga atttttcttc tggtccatgt agttcaatgc tgtaattgaa ccgatgctca
    14161 cctagacact gtgcttctgg tgttggaaaa tatccccaat cgcctagttc tcccacactg
    14221 cgaagtagcg tcactgcgat ttgtccgtct tctgttactt catattcatt caaaccaaag
    14281 tttccgactg ttacaccgta ctcttcatca tgaatattga caaatgcttg ctgatgttgc
    14341 ggattcgtgg ggttttccca acttttagac acatggtttg ggcgcttcac cacttcaaaa
    14401 atactatctg cttcgtgatg ttccacatgt aactttgtag gaaacaagac acgtaagcga
    14461 tgatctttca tctgattgtc gagagtcgtt tcaaagtcga tttttttact atcttttctg
    14521 attgtcatga ttgtttttat ttgaaggatt cgtttttctt tggatcgttc tgcttttcga
    14581 taacgaaatt ccacaaccat ctgctgttct ttttctaaac gctcgtctgc agaaacaggg
    14641 atttccattt cctgtagaat acttattttt gctatttccg gtgtatctac taatactttt
    14701 acttcagagt tttctttgtt actggatagg attggctgat cacagaaagg ttgtttaaag
    14761 atatactcat ttcctatatc gccgatatct tcaaaaatat gcagattatt caactttcgt
    14821 ttgtttttgc gatcttccat tgtaatggta ccattttttt caatcgtcaa atgtagaggg
    14881 ccgttttcga tgattcgtcc actctgatga atcattgttt ccttttcttc tgctttcgct
    14941 tccccttcta caagcgcgaa tgtttcccaa gaaaatgctg gcatgttttc taatggtaaa
    15001 gtcactttga tcattcttgc catatatggc acacggaaac ggtctttcgg caaatcataa
    15061 ccaaattgga cagtttcttc agataaaata gctggtatag tggccccact ttttgtttct
    15121 acatgatact tacgtttagg caaattttct aactcttgat atagttgttc aggtattcct
    15181 tcagcgaatc tttttctttc taattcgatg gttatttctg cttcaccagt tttatccatt
    15241 cctgcagtat ttacaatgac aaatggaaag ctgtcttttg ggaatccggt cgtatcgatt
    15301 gctgcggtca gctgttctaa ggaatcttgt gcaagatact ttccaacttc atctgctttt
    15361 tcaaaacgag gtatcatttc ccgatggaca gaatcgactg aacaaccgca aatactgtcg
    15421 tgaggatgat tttgcattaa tgtcttccaa gcataatcta attgatcatg gggatagttt
    15481 ccactaactt cataagccat cgtcgcaagt ggttctgtaa tattttctag ctgtcgctgg
    15541 accttggtat tccattgttt taagtaaacc cgagcagatg ctgtattggc taaagtatac
    15601 cagccatccg tttcttgact tgtcaattct ccttctactg agcctaagtc ttctggcaca
    15661 tctttttgaa ctgcttccag atagtcagta aaattagaat gaataaactc gtaatctgga
    15721 aacagttcat ttgcgagatg gattgctttt gatatatctt tttgtaccgg ttgatgatca
    15781 acaccattca tcatcaataa gtggttcgta gaggcatatt gttctgcatc cgctaatttt
    15841 tgcttccaaa aggcaagagc tgcttctttt tctactggaa tctcattccc gttgctatac
    15901 caattggcaa atagcagacc aaagattgct gtttgatcag gtcctttcca ccacatctct
    15961 gagtattggg aagaatagtt ttctgcttcc aaaacttgat tatcaaagcc aattggtttt
    16021 acaccacggc caaacgctgc tgctgaaata ccagcttgtt tcattaattg aggcgtctgt
    16081 cccatatttc caaatgtatc gggaaaatag cctaacatga caggggttcc ccatttacgg
    16141 ctttcttcca tgccaatcag catatttctc acgttcgatt ccgagctgat aagaaaatca
    16201 tcttgcaaga tataaaatgg tccaattctc aattttcctt cattgattgc ttgttggatt
    16261 gcctgtcttt tttctggcct tacttgaaga tagtcatcta aaataatcgt ttgcccatcc
    16321 aaatgaaaac tgttgaagga cggatcaact tcaaatagct ctagtagatc gtctatcaat
    16381 tcaatcagtc gcatgtgatg ctgttcgtaa gccatgtacc attcacgatc ccagtggcta
    16441 tgagaaatga tatatacttt ctttttcatt tacgatacct cctacttttc tactctaata
    16501 tcaaagtaat ccatgactaa ttcgcaaaac atcatatttg cccaagaaaa ccattctctc
    16561 gtataatttt caggattgtt cacatcgaat ccttcgtgca taaggtgtgt tcctgcgtca
    16621 gttgctacaa gtaaattcaa tatccgctct ttttcagatt tgtctgacgt tgtcattcct
    16681 tccatagcta atgcaatcgg ccaaacgtat ttttctggcg tatgggagga tccaattcct
    16741 tttgcgtact ttccttcata gaaatatgga ttttctttac tcaacaatgt tcttcttgtt
    16801 tgcagataat cttcctcttg ttcagaacaa tatcccaagt aaggtgcggc gatcagattg
    16861 ggcacattac tgtcatccat gactgtggcg tttcccattc catctacttc atatgcataa
    16921 atcgtttgcc ctttttgatt aaccgttcgt ccgtgattct gaatcccctc ttggatctct
    16981 ttcttcaatt tctgtgtttg ctgtacaatt tctttatcat cgagtatcgt ttgaaaaatt
    17041 tcttccacat aacctaacac gaccaccgca aacatattgg atggtaccag atatccataa
    17101 cggcaagtat catcgctagg acggaaacca gaccatgtca ttcctgtaaa agctgttttt
    17161 gcacctcgcc cgtcattgat cagcgtatct tcttttcttg tcgtgtctcg tacaaatgta
    17221 taaggagagt gttcatgatc ttgctctgtt ttgaacacgt ccaagatttt tttgatacct
    17281 tcaataaaag attgattgaa ttgatcagtt tttcctgtat ttttgtaaag aagataggcc
    17341 aactgtattg ggtagcataa agaatcaatt tcatattttc tttcccagat ccaatcgttc
    17401 atttttgtat gatctgtttg gtggccagca ccgttagctt cttcgttgaa ggcattcgca
    17461 tatggatcga tattgatata aaaaaattgc tttttgacta acccgctgat catgtcagct
    17521 aattcctcat cttcttttgc aatcactaaa taaggacgaa cttgtgcagt agagtctctt
    17581 aaccacatcg ctggaatgtc tcccgtcaat aaaaaggtgg ttccgtcatt ttgctttttg
    17641 actgttgtca acagagtatt ggcaaatgct gctgtaaaat tctttgccca gtccgcatgg
    17701 atatccccac atttttgtgt gatatcttcc ataaatttct gcaccgattt tggtatatct
    17761 ttataagcca ctaataaaac cttctttcta tttgatatat ctttaaagat tatacttcat
    17821 acagccaatt atgtctacgc tttcaaaaaa tatcttttta tttacttttt catccattat
    17881 ggtattattt gaatatgata tatcattaag aggtgtttat atgaatcaac cattgtacca
    17941 gacaatattt aatgatttgc aaacggctat tttaactggt gaattacctg tagacagcca
    18001 actaccaact gaaaaagaac tttcacgtac ctataaagta agcagaatca cttccaaacg
    18061 tgcattgact gaattagagc aattaggact tatttatcgc gttcgcggga aaggcagttt
    18121 cgttaaatcc gcttctccgg aacagattct tgcttctcct acaaaaacac gacggattct
    18181 gtttcttctt ccatatgtcg ctgatttgtc tgttggtgat tttagcaagg gattgaaccc
    18241 tgtgatgcaa gataaacaga ttgatgtcat gatgacgacg cttgactttc tacaaaataa
    18301 aacagcaaaa gagctaatca atgaatttga cggattgatt tattatgctc ttgatacaga
    18361 acaacatctt gacttacttt ttgagctttc attgaaagaa ttccctgtcg tcatattaga
    18421 taaaaaaatc tatgagttac cttttcctac cgttctttcc gataatttcc aaggcggtgc
    18481 gcttgctgcc tcttatttga tcgaacaggg acattcaaaa atcgcttatt tgtttggtac
    18541 gcagactcat ccacaatccg ttcgacagcg ttaccttggt taccttgaag cattggacaa
    18601 agctggtttg actttccaca cttcactgga tgacaaaagc gcgacattag atgaattgct
    18661 ttcttatatc actaagtatg aagtaactgc ttttgtttgt gaaaacgatg ttgtcgctat
    18721 ccagaccatg aatctcttga gacagcatgg ttatcatatc cctgaagatt tttcaattat
    18781 cggatttgat gatatccaag cagcagctct tgttgacccg ccactgacaa ccatcgcaca
    18841 agattttgaa cagttaggta agcttgcggg agatgctttg gtcacttggt tagagacaaa
    18901 agatcgacca tccgatagta aatttccagt ttcattcatc aaaagacaat caacaaagga
    18961 gaaataaaat gaatatcggt caaatagata ctagacatgc aacagcgaat cagccaaatt
    19021 tctctaatgg gaactgccag ccttatactg gcgttccttt tgggatgaat tattttgcac
    19081 cgcaaactac ggatcaaaac ggcagctggt ggtttcatcc tgatgaccgc gtttttcaag
    19141 gctaccgtct tacccatcaa ccaagtcctt ggatgggtga tttcagtcat atgctgttta
    19201 cgccgatcaa tggaaaacta caggagaaca cccttttcca tgctcaaagt tcttaccgcc
    19261 cagaagaaag tatattttgc ccgactcatc tgtcgatctg tcaattacgt tatggcatct
    19321 gttctacatt gatacctagc atgtatggcg gaatcttatc tattgattac agcaaaaatg
    19381 acagcggttt acttttatcc tttccgggac gccatcagct ctttgttata gatccttaca
    19441 cagtggaagg aaaaatcaca cattttgctg gttcagaaga tcctgatttt tctttttatt
    19501 ttatattgag atttgaatat cctttaacaa ttgatgagtt agctgtttcc aacaaaaata
    19561 gcgactcgat cccgttgtat tttgaacaga caaaaaaaca aactatccga tttgggacat
    19621 cttttatcag ctcggaacaa gcagcttata acctggaact agaacaaaac caaagcaaag
    19681 agacttacct tgctcacagt caagaacaat ggcagtccta ttttgatcgt atacagctca
    19741 cacaccatga tcaacaacaa ttgcggacgt tttatcataa tttatatcgg gtgtttttat
    19801 ttccacaaac attttatgaa attgatccta gtgggcaaaa aatccactat gatacgacta
    19861 gtcgatctat aaaacctggt gttctctata caaataatgg gttttgggat acgtataaaa
    19921 cggtttatcc tctttattct ttgattgctg tagaaaaata cgaagaaatg ctggaaggtt
    19981 ttttgaacag ctatagagaa agtggctatt tgccaaaatg gctgtctcct gatgaacgtg
    20041 gacttatgcc tgggacattg atcgatgcgg tcattgcaga tgctgctgta aaagatatcc
    20101 gacgagactt gatgcctgaa tttctaacgg caatggaaaa aggtgctacg gttcaaagca
    20161 aacatccaaa ttatggacgc caaggaacag aagattactt gacatatggc tatgttccta
    20221 atcactacca cgaatcagtc aatcacacat tagactatgc ttatagcgat ttttgtatta
    20281 gccaagttgc caaggtacta gaagaagagg aaatgacgaa gaaatatcag cagcaatcat
    20341 tgaactatca aaatatcttt gactatgagt caggatttat gcgttcaaaa gataaagatg
    20401 gcaagttcag agaaccattt tcagctaatc gttggggaca agattatgca gaaggaagtg
    20461 cttggcaaag cagctttgct gtttatcacg atttttcagg actgatcgga aaatatggtg
    20521 gcagtgagag atataaagaa aaactcattg agctgtgcaa tcagccccca acgtttgacc
    20581 caggaggata tggttttgag atccacgaaa tgagtgagat ggccgcgata gaatttggac
    20641 aattagctat ttcaaaccag ccaagttttc actatcctta cttgttcagt tatataggac
    20701 aacctgaaat ggcacagcca ctgcttaaac agttgatgac tcagaccttc gacgattctc
    20761 caatgggtta ccctggagat gaggacaatg gaagtatggc cgcttggtat atcttcaata
    20821 gtctgggatt ttatcctgtc acgcctggaa ccggtgaata tgttatcggt atgccattaa
    20881 ttgacaaggc tgttttgctt ttgtcaaatg gaaaggaatt gacgattgaa gcaacgccta
    20941 atcggccgca gcaacaattt atccataaaa tcaaacggat ggacaaagaa tatacgaaat
    21001 tatattttac ccatcaagat ttgctgaaag gcggcacgat ctcttaccaa ttagggatca
    21061 tccctcaccc taagaaatat caagcaaaag atttgccttt ttccttatca aactataatt
    21121 aacaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    21181 gatcctcata tttttcgttt ggttggtctt attaccgcag atgcaagctt ttgaacaccg
    21241 tttattgaga ggttgtgaaa tca
//
LOCUS       DACCCM010000050        20802 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCCM010000050 DACCCM010000000
VERSION     DACCCM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.053"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(114..1565)
                     /locus_tag="IVK78_001805"
     CDS             complement(114..1565)
                     /locus_tag="IVK78_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6375278.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(1562..3286)
                     /locus_tag="IVK78_001806"
     CDS             complement(1562..3286)
                     /locus_tag="IVK78_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6375279.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(3279..3893)
                     /locus_tag="IVK78_001807"
     CDS             complement(3279..3893)
                     /locus_tag="IVK78_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6375280.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(4238..5695)
                     /locus_tag="IVK78_001808"
     CDS             complement(4238..5695)
                     /locus_tag="IVK78_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6375281.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(5736..6656)
                     /locus_tag="IVK78_001809"
     CDS             complement(5736..6656)
                     /locus_tag="IVK78_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6375282.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(6668..7615)
                     /locus_tag="IVK78_001810"
     CDS             complement(6668..7615)
                     /locus_tag="IVK78_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6375283.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(8094..8813)
                     /locus_tag="IVK78_001811"
     CDS             complement(8094..8813)
                     /locus_tag="IVK78_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6375284.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(8862..10508)
                     /locus_tag="IVK78_001812"
     CDS             complement(8862..10508)
                     /locus_tag="IVK78_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6375285.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            10687..11277
                     /locus_tag="IVK78_001813"
     CDS             10687..11277
                     /locus_tag="IVK78_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6375286.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            11369..12874
                     /locus_tag="IVK78_001814"
     CDS             11369..12874
                     /locus_tag="IVK78_001814"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP6375287.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(12958..14310)
                     /gene="celB"
                     /locus_tag="IVK78_001815"
     CDS             complement(12958..14310)
                     /gene="celB"
                     /locus_tag="IVK78_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6375288.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(14310..14828)
                     /locus_tag="IVK78_001816"
     CDS             complement(14310..14828)
                     /locus_tag="IVK78_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375289.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(14844..15170)
                     /locus_tag="IVK78_001817"
     CDS             complement(14844..15170)
                     /locus_tag="IVK78_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP6375290.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(15183..17162)
                     /locus_tag="IVK78_001818"
     CDS             complement(15183..17162)
                     /locus_tag="IVK78_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6375291.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(17183..17497)
                     /locus_tag="IVK78_001819"
     CDS             complement(17183..17497)
                     /locus_tag="IVK78_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP6375292.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            17665..17958
                     /locus_tag="IVK78_001820"
     CDS             17665..17958
                     /locus_tag="IVK78_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP6375293.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(18036..19149)
                     /locus_tag="IVK78_001821"
                     /pseudo
     CDS             complement(18036..19149)
                     /locus_tag="IVK78_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            19302..19526
                     /locus_tag="IVK78_001822"
     CDS             19302..19526
                     /locus_tag="IVK78_001822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375294.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            19536..19715
                     /locus_tag="IVK78_001823"
     CDS             19536..19715
                     /locus_tag="IVK78_001823"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375295.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            19909..20079
                     /locus_tag="IVK78_001824"
     CDS             19909..20079
                     /locus_tag="IVK78_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6375296.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20503..>20802)
                     /locus_tag="IVK78_001825"
     CDS             complement(20503..>20802)
                     /locus_tag="IVK78_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6375297.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         6417 a         4166 c         3510 g         6709 t
ORIGIN      
        1 ccgtttatcg agaggtcatg aaaaagctgc cttgcctcaa gtaataagaa cagcccaaaa
       61 ttagatttct agacaatttt ttataagtta tttcattaca ttggcttttt cttttatgat
      121 tctccactgt tttctcggta ctctttaggg gatctgccga cgattttctt gaataattta
      181 gagaaatacg tggtgttgtt gtagccaatc tcacttgcta tctcattgat gttttgatta
      241 ctgtgcaaaa gcagttgttt cgctttttcc atacgtaaat ggttcaaata agctgaaaaa
      301 ctttttttcg tttctttttt gaacagctgt cctagataca tgacatttaa atgcaggttt
      361 tctgacactt ctttgagtgt aagtggttct tgataatgct ggtgcaggat tacgattact
      421 ttttccacat tgtcactgta ctttttctgt ctctgatgct ttttgatgaa tgacagaagt
      481 agctgctgaa gttcttgcac cgtttttgcc tgatggattt tttcgattcc ttgtaaatat
      541 tcctcatctt ccagatcaat caattcccgt tgaatctcca taaacagtaa aaaactggaa
      601 tatcgtatat cttccggcat catggctgct aactgaaact gttcaaaaaa atcagtgatc
      661 attttttgag cgacttccaa ttgtttattt tgcagcacgc gtctgaaact tgaaaaatcg
      721 atggtttgat cttttgtttc tgcttgtact gcgtaaaaaa catgctcgtc tttatctcca
      781 taaaattgat gcaactgtag attgtctttc gcttgttgga atgtttcagg gattttgtcc
      841 aaagaatctg tctcttcccc gatactgatc agccattcct gatcaggaaa gttttcttga
      901 acaaaacaac agaagccatc aacttcatct gcctccaaca acaacgtgat caatacacgg
      961 tctccgtaat ttcgttgata gtaaaagatt tgttgccgtt cttgcaacca tctattgata
     1021 agagtatcat gagttcttgc cagttgaatc aatactatcc tatgctgctt ctcacttatt
     1081 tgtcctatta tttcttcttc acttatttca tccaattctt cattcaacca ttgactgaat
     1141 aacagttctt gatagatttc ttgttgattt ttttgttcat tttgctgatc taaccgtgtt
     1201 ttgattttct tgaggctttc aaccaattcg aacttattca cgggtttcat caaataattc
     1261 acggcgccta attgcatacc tcctttcaaa taatcgaatt cttggtatcc tgataaaatc
     1321 ataaattcaa aattatgacg ttctttttga gccgcttcaa taaattccag tccatttatt
     1381 tctggcattg tcacatccgt tactactaaa tcaatctgtc gctgttcaag tacagctagt
     1441 ccttctttgg cattctctgc tgttgctact atttgaaagc ctaaactttg ccaatcaatt
     1501 atctttttta acccattcaa aatcatgtat tcatcatcta ccaataacac ttgatacacg
     1561 ctcatccctc cttgaatgta atagaaacgg tcagtccacc tgtctcatta ttctcaagcg
     1621 acatataata actgctgccg aagctggcac gtaaacgttc attcacgttt tgcagaccga
     1681 tagaaccgtg aagttccact ttaggatgag acagtttttc ttcaatctgt tttagccgtt
     1741 tttcagtcat cccttttcca ttatctttga tgatgatcct tacccgtttg ccttcctgca
     1801 agactttgac acttagcgca ttatcgaatc gagtaaaatc aataccgtgt ttaaaataat
     1861 tttcaactaa tggctggatc acaaatttag gtacttcgat cttttccaaa tcaggatcga
     1921 tcataaaatg ataggctact cgattaggat aacgcatttg atacaaatag acatattttt
     1981 cgcaaaagtc cagttcttct tttaaagtga tcgttttctc ttgattcgta ttgtttcgaa
     2041 gcaaagcgga aaacgcatac acaacatctg ctaattcttc acttccttcg ctgattgcat
     2101 acattcggat atattctaat gtattatata aaaagtgtgg attgatctgt gcttgaagcg
     2161 cccgcatatg tgcgtcttgc tgtttgattt ctaatttgta gatgtcttct acaaactgat
     2221 tgatgctatc caacatttcg ttgataccga ttgaaagttc cttcagttca tattccgtgt
     2281 ttgttatgtc gatccgcgtc tctaaattcc ctgctgctgt ttgatccatg gcctgcatga
     2341 cgatagatac ttgctgtgaa tatcgtttga atgttttata aagaaagata agcagtatat
     2401 tgtctaaaat aatgatacca atgatcagcg gggcaagatt ttttcccgtt tcgattttta
     2461 cacttttttt atctagtaca gctaatatac gataatcatc agacagttct tggtgttgaa
     2521 caaaattgtt tttttccaga tcgtcaatcg gcaattgtga atttttcttc agactctctg
     2581 tgatgatcat ttcttgtctt tcaaacgttt ctttagctat tttattatca tggaatgtgt
     2641 atagtcgatt tgtcgttcca gaaatcatgt ataaagagag tccattgaat gacgttagat
     2701 tttctaaaat catatctagt tctttctttt gaaaaccaat aaaaatactt cctgactcag
     2761 ctcctccttg tgtgataggc aacttgatat aaaaggcgtc tagtaatttt ggtgtcccct
     2821 tttttatttc cccacctttg ttttctctag aagattcata atactccggt agttgattca
     2881 acacgatata tatcgcactt agttcttcat aatttgcgta gagatctttg atttgatttg
     2941 ggaaagaaac taattcatcg gtactgtttt gctgttcgta tacatacatc agatactgat
     3001 ctatgggttt tgttaagtag gtcgtcacat tttcgatctt ttcggaattc cctacaagct
     3061 ggtttttgat gatcctactc aaggttgttt tatcattcag cattctgctt gtgctttgaa
     3121 cggcatcact tgtcatgttt tctgcctgtt tttgattttg ttcccaagta tggatactaa
     3181 aaataacggt agcagtcatc gttacaccga tcatgataac cgcgtatttc cgcaacagac
     3241 gatttagtaa ggttcctttt gaaaatagaa tccgaaactt attcataact ttccaactgt
     3301 ttatctaatt cttcacgcca atgtgttgtc atcgtaccat tccaaaccgt caaaagacca
     3361 atgacaccaa acaggatcaa tcctttgaat tgccaagtca cgcctaaaat tataccgcta
     3421 ccaataatta tttttagaaa agacgaaaag ctggaaaaaa ccgaaataaa acttagcttt
     3481 agcagattag gaacagagat cgtatattca ctatctaaaa tcatactgta ttgataagta
     3541 gcaaataggc agagcaaggc aaaaagtaaa ataaaatcaa tagctaaaaa aatgagccct
     3601 ttgatttgaa cagataaata taaattgtaa ctcaataaag cagtaccaga taaaaataaa
     3661 ctaaatagca gattgcctct taaaaaattt cttttatata tattccagcc tctttttatt
     3721 gtgatttctt tatactcgaa tccgtacaaa taaaatagtt cattgactgt tttccatgcc
     3781 gcacctaatc caaagatgat cccgccggca aatgtatata tccagaaata caaggttagt
     3841 tttataatga cccagatccg gataaataac tcttccaaaa ccttccctaa catatcttct
     3901 cctccaaaca ttaagtttat actttctcat tatacaaaaa aaggttgggc aatcagcacc
     3961 ttgacctgat tattagagaa acaaacggaa aaatagttcc tcatattttg tcgttttgtg
     4021 agcgaatacc gagggttagc tgattgaaca ccgtttacca agagtttgtg aaatagccgc
     4081 ttagtttcga gtagtaagaa aaattttgtg aatatggctt tccacatttt tacatatatt
     4141 tagcttaata ccgagaaact ggcttttgaa caccgtttat tcggaacaag aggttgtgac
     4201 acgtcttttg tcacaacccc tttgtcttta ttcattatta ttcttgtcgg aactcatcat
     4261 attgtttttg catttctttc aataccttat catagccagc cttgtccaaa gcgtctttca
     4321 atttcggtaa tgtttcatct ggatcaacag tccctgtatt caatccatct aaatattgat
     4381 tcatgacatt agaaatatta cttaattctg ttttgacact gtcagtgttg aagctgaatc
     4441 ctaaaatcgg tgaagtttct gcatcagcaa tcgattgatc acgtttggca atcatgtcat
     4501 ccgtaataga ctcttgtgta tatagaatct tgttgtttcc tgtattccaa gcagacatat
     4561 gtgtatttgg ctgataacca tctagcaatt tgattttatc atctgaacca tcgacttttt
     4621 cccatgcttc tccctcgatc ccccaaacaa gcccattcaa taattctgga tcactgttaa
     4681 gcagccctag aagttctact gatttttctt tattttttga agtatttgat acgacaaaat
     4741 ttgccatttg tgcttgagct gtagatttta gtggaacagt aattgctttt gagacaagtt
     4801 cttgttttgc tgcatttgtc aaaattgtat cgccataatc atacggtcct tgtgtttctc
     4861 cacgcattag ccaagtattt ccttctagtg gataatcctt attgctcgtt gcagcgtcac
     4921 ttggaatgag tccttgtttg taccagctat gcattgtacg taacagtccg atatacgttt
     4981 cattatcgta ttggttgatg attttcgaat catctccatc cagatcaata gcaaatggta
     5041 acgtattccc taaaggataa tcaaagttcc cttggacttt gaagccttgt ccaatggcaa
     5101 aagctgccgt gtttggttct ttttcatgga attccttcaa gactttttct gcatcagcgt
     5161 aagattggat tccatcaatt gaaagatcgt atttatctag taaagctttg ttaaaagtaa
     5221 gtacttgttg agcaaagaca tttccattga ctgggaaagc atataatttt ccattgacca
     5281 gatttccttt aatatatgct tcatctaaat cattataagc tttttcagcg tacttaggcg
     5341 ctaattcagt caaatcagca aaggctcctt tttgggcatt ggaaacatag ttgttcgcaa
     5401 aggcgatatc ataattttcc ccggaagaaa tgatgacgtt cattttcttt tcgtaatcgc
     5461 cccagccaat gtaattgata ttgactttta caccgatctc tttttcgatt cgtttgtttg
     5521 ctacttccat caattgatcg aaattatctg gtttatctcc gatttgatac atctgcaaag
     5581 tgtccgaatt gctagcttct gcctggtctt tagaaccaga acctccgcat gctgctaatg
     5641 ttcccaacaa caacgctgaa acacctgtaa aggctagttt cttccataac ttcatttttc
     5701 ttcctcctaa ttttatgatt tatatttttg tttctttatt cttttactcc gccgattgtt
     5761 agacctttga caaaatgctt ctggaaaaat ggatagctga tcgcaatagg caacgtcgag
     5821 atgactacga tcgccattct ggcagattct cctggaactg aggctaatcc gcctgtcaat
     5881 tggcttcctg ctcctgaatt ttgtgtcaga tattgaatat tactttggat tttcatcaaa
     5941 agatactgta gtggaactag cttatcatct tggatgtata gtaaagcatt gaaccagtca
     6001 ttccagtaac ccaatgcggc aaataaactg attgtcgcaa tacctggaac agctaacggt
     6061 aaaacgatct gaacaaatat tcgcagttcg ctagctccat cgatccgtgc cgattcaata
     6121 atcgaatcag agaccgaacg tttaaagaat gtgcgcatca caataatatt gaagggactg
     6181 acagccattg gcaagatcaa tgcccaaatc gtatctttta actgaagaag atttgtcata
     6241 acaagataat tggccaccat ccctggtgag aacaacatcg taatcaaaca aaaaacggta
     6301 aaaaaacgac ggaatggaaa gtttgaccgt gaaatcgcat aagcatataa agaagtagcc
     6361 gtgctattga tcagtgtacc taaaacagta acaagtacag tgacgaaaag cgcttggaaa
     6421 attttatcct gcatctggtc gaacaagtac gtataaccaa atatactgaa cttttctggc
     6481 caaaaactgt acccgtgagt cgacaatatg gactcatcgg taaaagagat ggcgataaca
     6541 aatataaatg gcaaaataca ggaaatagca aagagtgcaa tcattatatt agagaaaaaa
     6601 ttagccgtct tattgaaaga gcggacttgg actctctcca ctttcttttt tttcattttt
     6661 ttcctcctta gaataatgca gaatcgctat caaaacgtcg ggcgatcgta tttgtgatca
     6721 tcaacaagac aaagccgaca actgattggt aaagtcctgc agcagccgtc ataccaaaat
     6781 ctcctgtgga tgtcaggcca ttgtagatat acgtatccaa aacttgagtc acatcatata
     6841 atgcaccgga attcctagga atgttataaa acaggccaaa atcagctcgg aaaatgtttc
     6901 ctacggctaa aatcgttaaa atcgtcatta gtggaataag ttgtggtatc gtcacatgtt
     6961 tgatctgctg ccatttgcta gctccatcaa ccatagcagc ttcataataa gtcggatcga
     7021 ttcccataac agatgcaaag taaataatgc tgttataacc aatccctttc catgtaccaa
     7081 tgaacaccaa gataaacggc caatatttcg gttcattata ccaattgaca gcttctcctc
     7141 catgtccagt gatccactga ttgaatattc ctttgtcagg acttaaaaaa gcgtaaacaa
     7201 agtaactgat aatgacccaa gatagaaaat aaggtagtaa ggacatcgtt tgataaactt
     7261 taacgagtcg tttatttctc aactcgctca taatgattgc aaatacgact gagatcagca
     7321 gattcaaaag gataaatgct acattatata aaattgtatt tttagtgatg agaaatgctt
     7381 gatccgaaga aaataaaaat ttgaaatttt cccaacccac ccaaggactt tccctcaagc
     7441 tagccaagaa cccatctgct gaaatatgaa aatttttgaa tgctacgaca ttcgccatta
     7501 caggtatgta aaagaaaaaa atcatccaaa gcatacccgg tacagccatc aggacaagtg
     7561 ctttataacg ccatagattc ataaaaaaat gattgaaccc ctttcgtttt ttcatctaaa
     7621 aacccctttc gattcattcg caacgtaatt atagcgcttt caataatgag aataaataaa
     7681 caaagtatag ggaaaatgaa acaaagtata ggagaggttg tgaaatcagc gttttggcct
     7741 gagtattaga gaaaaaaacg ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     7801 accgagggtc agctgattga acaccgttta tcagaggttg tgaattcagc gttttggcct
     7861 gagtattagg gagatcgtgt ccttactatc taactctgag tcacaaggaa caatggggac
     7921 tgtaacaaaa gtcttgtcac agtcccttat tccgaacaaa cggtgttcca gaagtagctt
     7981 ctacggaaat aagccgaaat ttcacaaaaa agccatccga aagtcagacg gacaagtctg
     8041 cttttcagat ggcgctacaa ctactctgct tattctttta gctatccatt caattatttt
     8101 tctctcactt gttctagctc ttctttggtc atgggtcgat attgcccttt attcaaatct
     8161 ttaggcagcc aaaattttcc catacggatt ctttttaaat ataagacttc ttttcctact
     8221 gctttgacca ttcgcttaac ttggtgaaac tttccttcat gcaaaatcag acggataatt
     8281 gaagtttctg tctcttgatc tacttgatca atttggagtt ttgccggcaa tgtctgctca
     8341 tcgccatcaa taatcagtcc ttctgcaaat aaacggatat cttcttctgt cattataccg
     8401 ctaacctcag ctagatattc tttctccaca tgttttttag gtgatagtaa ctgatgggca
     8461 aacaccccgt cattcgttaa aatcaagagg ccctctgtgt ctttatctaa tcggccgact
     8521 ggaaaaagat cttcccgata atcctggtct tccaataaat cgattacggt ttgatcatga
     8581 ttatcctgag tagcagaaat gactcctgca ggtttatgca aaatgtagta aaaatctttt
     8641 tgatacgtaa tgatttctcc taaataagta accgcatctt cgaattcatt tacttgcgct
     8701 ttatcgcttt tcaccacttg tccattgact tcgatcgttc cttttttgat taatcctttg
     8761 acttctttgc ggctgccaag tcccatatct gctaaaaact tatctaaacg catgcgtttc
     8821 ccccatttca aaagagcatc aatctagctc ttctgattca cttattttat ttttaattta
     8881 tgtcgtaaac gcgctattcc tggtcctagt agtctttctg ccaagcggat tttcaaagct
     8941 aaataaccat acactaaagc ccctatacct gctactacaa gaatcaacaa tagagactgg
     9001 aatttgctat cttcgtttaa aaaagcacca aaaatcatct ttgtgatcca cgaagccaaa
     9061 agcataatca aggtaagaat aaagatcagc aatgtcctgc gccagacaaa acttggttta
     9121 aagcgggcta cttcatggat tctctttaag atcaaggtac aagacacagc aaatccgatc
     9181 attgtcgcca ataaagggcc gtatacttca aataaacgaa tggctgggta ttgaagaagg
     9241 agtttcacta gaaacccaat aaccaaataa ttaattgccg ccttgttttc aaacattcct
     9301 tgcagcatgt tggaaactag catatatagt cctaggaaga gtcctacaaa acttgcttgg
     9361 atcagtacat tacttcctag agaatccggt gtataaaata atgtatttag cggataggca
     9421 agtaacataa caccaaatgt cgccggaaac ataaaaaatg aaaacaactg taaatttccg
     9481 cttgtcaatt ttgccagacc tactcgatct ttcaacgtaa cagcttctgt aatcaacggt
     9541 aacccagtag acgcgatcga tgttgccaat gcaataacta ccatcgtcaa cttatctgga
     9601 ttggcactaa aaatcgaaaa aagatccagt aactgtgcat tggaatactc ggtggatgca
     9661 gacatgatct tcataaaagt gaactgatcg actaatttga agatcgttac ccctgaaccc
     9721 acaataataa acggaatcgc ttctttaacg gtatctaaca acaattcctt cgttgcaata
     9781 tttattttgt tttcgctgta gtgaataaac gccgatgtat acgcttgatg tttcttcaaa
     9841 aaatacaaaa ggacaagaat acttgctagc ataccgataa atgccgcgaa agttgattgc
     9901 gtgaccgccg tcacatagtc accatccatg actttcatga tgatgaatgt agccaatagc
     9961 atataaaaaa tacgtgccac ctgttctaca atctgcgaca gcgcataagg catcatttct
    10021 tgattgcctt gcaaataccc tcgtatcaca ctcatacaag gaaaaaccaa tacagcgata
    10081 ctcaaagaac gcatcgttgg aatcagttct tctccgccac cagaagcatg agctagccaa
    10141 ggagaggcga tgtacatgaa caaagcaaac aacaggccta acccgcccat catctgtaat
    10201 gcacggataa ataatcgtcg tgaagtgcca tattcattca atgaattata gcgtgcagtt
    10261 tgttttgcaa tcgcagcagg tattcctgct gtagagatca gtagaaacaa tgcatatata
    10321 ttgtatccca tattgaataa tccattggca gccttagcat tttcgcccat ccaagcatac
    10381 cagggaatga tatagacagc gcctagcaat cttgaaatga tattactggc ggtcatccaa
    10441 gcagagcctc gtgccatttt ttcttgcggt gttaattgat ctaatgctgg ttttctttga
    10501 ttatccatcg gtcagattcc aactttctac aaaacattac tatcatttta attggtaaaa
    10561 cagaaactgg caatcgattt tatataactt ttataagaac aattgttttt ttgaattcgg
    10621 tttcgttttt ttggtaaagt aaagaaatat acagcctttt atggctaaca tttataggag
    10681 atatcaatga cacaagaaat aaaaaaacta cccgttatat ttttaggtct tttctttatc
    10741 acagttggat tgaattggtt cttgctccct cacgatatag cttccgccgg agtgggcgcc
    10801 atcggacatt tagttgaaac ggccttttct atccaacgct cagttactgt ttggagcatc
    10861 aatcttatga tgctgcttct agccgctgtc ctattaggaa aatcagtatt ttttaagact
    10921 gtagcgggaa gtctgctttt tcctgttgtt ttagagattg ttccaaaagt ggaaatgatc
    10981 tcctcacatt tcttttcact gatttcagga agtcttttgt tcagtcttgg ggtttacaca
    11041 ttgtacacag tcggggcctc aaatggcggt ataacgatac cgccgattat tttccacaaa
    11101 ttttttcggc ttccaatgcc gcaaggactt ttacttacca atagtcttat cgtctttttg
    11161 aattattatg tttttggatt gttggagaca ctattcgttc tcctatctat tacagtcagt
    11221 tcattgttta tgaaaatact catccgcttg tcccctgtgt caagaaaatt tgaataaagc
    11281 tggattttgt catagcggaa acaaacgata tattaaaaag agtgtgctat aataaaccaa
    11341 gaaattgaat aaagcgaggg atcaatcaat gagcttctct ttagaagaca tccgtcattt
    11401 actattaaaa gaaaatctat taaaagaatt tgtttcaaca agaggctggc atcttgatgt
    11461 accagaaaaa gctgaatttc ttcagttaag ttatgattca agaaaagctg atgcttctac
    11521 tttgtttttc tgtaaaggaa tgaatttcaa agaagaatat ttggattcag caatcgaaca
    11581 gggtattcaa tattatgtat cagaacaacc atacgaaaat tcagcagtag gaatcatcgt
    11641 aacggatatc cgtaaagcaa tggcgcttct tgctatggct ttttacgact atccgcaaaa
    11701 taaattgaaa gtgatcggat tcactggaac aaaaggtaaa acaactgctg cgtactttac
    11761 aaaagcaatc ttggatcaca caacaaataa aaaaacagca ttgctttcta ctatgaatac
    11821 aacgttagat ggcaaaacat atttcaaatc ccatttgaca acgcctgagt cattagattt
    11881 atatcgtatg atgtcagaag cagttgaaaa tggtatgact catctagtga tggaagtttc
    11941 ttcacaagct tacaaaacac aacgcgtata cggcttgact ttagatgtcg gaatcttctt
    12001 gaatatctcg cctgaccata tcagtccgat cgaacatccg acatttgatg actatttcta
    12061 ttgcaagcga caattgatct tgaattcaaa aacagttgtt ttaaatcatg aaagtgacta
    12121 ttttgattta cttagggaaa cagccgatct attccagatt ccaacgatca cttatggtcg
    12181 ttcggaagaa agcaattatc aagtgatccg ttcagataaa ggtactcacg gttttacttt
    12241 atcttctcat gaagatcgct tagctatctg cgatactgct tatgatattt tgctggctgg
    12301 aggattcaac caagaaaatg cagccagtgc gatcattgcc gcagcactaa caggtgcctc
    12361 cagtcaagat gcacaagacg gattaaaaga agctcgtgta ccaggccgaa tggatcaatt
    12421 gatccaatcc aatggagcgc atgtctatgt ggactatgca cataattatc taagcttgaa
    12481 aacattgctg aaatttgcca aaaatgaaca tccagatggc cgtgtaattg ttgtattagg
    12541 cagcccagga aacaaagcaa tctcccgtcg acatgatttt ggaaaagtgc tatcagaaac
    12601 agctgacatc gcctttttga cggcagatga cccagcattt gaagatccga aaaaaatcgc
    12661 ggaagaaatc aatgaagcaa ttactaatcc agatttgatc gttcattatg aaatggatcg
    12721 tcctgaagcg attcgacgcg ctttagcaga aagcgctccg caagattcag tcgttattgc
    12781 gggaaaaggc gtggatccct atcaaaaaat caatggtgta gatgaacctt atgaagggga
    12841 ttatgcaatc gtgaagcggt tgattgaaga ataatggtat ttatatacta gtggtttccc
    12901 atttctaaga aaaaaaggga ctgtgatttt ttgtcacagt tccttttttc ttagaaatca
    12961 ttttaacttt tcactagcta attctgctgc cttttctctt tcttcattta ataatttatt
    13021 atcatagaat ttgataaatg ggaaccagat aatgaatgcg atgattgcac aaatgactgc
    13081 taacacagca gctttccaat caccaccact accaacaaat gctccgatcc cgactggtga
    13141 aggccacggc atctgtgcaa gcatcggttt gacgatttcc agccgaatgg caaaataagc
    13201 gagtgtagca gaagccatcg gagctaagaa aaacggaacc gctaaatagg gattgtagac
    13261 gattggcatc ccgaaaatga ttggttcatt gatattgaat aatcctggta caagtgaggc
    13321 ttttcctaac actttcagct gatcagattt cgcgaggaat gcaataaaga tgatcaatcc
    13381 taaagtcgca cctgatccac caacggttac ataagaattg ttgaattctc ctgccaacgg
    13441 ataatttgcg ccttgtgcgt tagctgccat attagctaat acgatcgggg tcaagaaaga
    13501 agtgatgata ttcgctccat ggatccctac gatccacaat gcatggatca agaaataaat
    13561 caccatgaca ccaagccatg tattcgtcaa ctgcgtaaca aacccaaatg ggatagcaat
    13621 cattttgaag atatccgttc ctagtgcaac taataatcca ttgatgatca tcaccacaaa
    13681 cgcaatgaca aatgttggga tcagagcagt gaaagaacga gaaactcctg gcggaacaac
    13741 atctggcatt tttatgaccc aatttctctt gacacacata caatacaatt cgaccgcaat
    13801 aatcgacata ataatagctg taaagattcc tgaagttcct aaacgttcga caaaagagcc
    13861 catcctaata ccatttacta cagtttctgt ctcattgata gaagcaatca agctgatttt
    13921 accatcagtc atgatcaatt cagggataca cataaagaat gcaaaaacag atagtaatgc
    13981 ccccgtgagc ggattaacat ttaatttttg ttcatctcgt tcaattgacg ttaattcata
    14041 agcaaacaca atggcaaagt aaatagataa aatccccatc gtcattgtat tgacgatcat
    14101 gtacaagtca ctaactttga aaaaagttgc attaaaaaat ccttctaaaa atgggaacgg
    14161 taatggcaaa atatttaaaa ccaaaaacat cgagcctaca atcgtgaaag gaatagaagc
    14221 catccccgct gccatgacag cccgaacgat tttaagttgc gcaattttcc ccataggtcc
    14281 catcaggtat ttctgcaaaa aatcaaacat tagttattct cctttcctaa atataacgct
    14341 ttttcatatt gtttaatacg gatatactgt tggtcttggt tgagtgaaat tttctttaaa
    14401 cgatactgta taaatgcact ggcaaaaata ccgcttacaa caagaatgaa aacaaataac
    14461 tgtcctccat tgttatttgc cataaaagga tacaagaatg taaatcctga ggtaaacaac
    14521 actggaatca ctaataaatt aaagacactc tggaacaaaa aataccactt tgtccatgga
    14581 acgttcgttt ggtgttttcg ataaagcttt acttgttctc ctactgcagg caaaatagct
    14641 aaaatcagaa tcatcggtaa actacttcct aaacttttag ctgatagaag cagaatcatc
    14701 cattgcatgt tcatgaaaaa gaaaaaggca gtcaggtaac gtacgattag ataacgattg
    14761 aaatacatgt ttttcaaact aagttgcttt ttttctaatt cgattttcct ttttactgta
    14821 tcctgcacaa tgatccttcc tttctattta tttttttgat acaaataatt catttcctgt
    14881 gcaacttcca ataatgtcat cgtcgtcatc agatgatctt gtgcatggac catgataatt
    14941 tccatttcta tttttgtccc gctggcatat tctttcagaa gttgagtttg tgactgatgc
    15001 gcatgaagaa tctcttcatt tgcttgtcgc aacttctctt ctacttcttc aaacatctct
    15061 ttacgcatcg agcgaaatgc ttcatgtatc agtgtccgtg catttccgct atggaaaatg
    15121 atatcaaaag aagtcacttg tagtgtttca ctgtttattt tttcagccat ctgttttcct
    15181 ccttattcga tcagtttgat aaatttactg ctgaaattct caaaaatagg ttcagctaag
    15241 atttcctgct gtacttttag ctggtcaact aacctaacga ttgctttagt cacgaccgta
    15301 attccttcat tttcgacata agaaggcgaa actaaaaaaa caaaatgaat ttcttgatat
    15361 tgatcccact gcatcccaga aggaatcaat gcaacggcta cctttgtttc gattccaact
    15421 ggaacagcgg gatgtgggac tgccactgta tcactgaata tgatttgtcc catattttca
    15481 cgttgctgga tctgctttcg catttctctt atatatcctt cttcttcatt tatgctcagc
    15541 ttttgcagca aatcatgaag aatttcttcc tttttcggtt cgtacgtata agtcttgaaa
    15601 aatttttcag acaactgttc ggtgtaaatc tgcttttttt gctgtagaga aaatgctggt
    15661 atcggtctat ctgaggaatg atagggtata gattctccaa ctacctttcg tattttacga
    15721 acatcatctt cattcaaaaa aatactgaca tgtaaaactg gaattttgaa aaacattgtc
    15781 gaaagatcaa tagaagaaat aatcaggtcg attcctttta aagttgtttc attgatctca
    15841 taatagcctt ttacacttac cactgtaata tttttaccga attcactgac tacgcgattc
    15901 tttaatagtt gggcactgcc gtaacccgtt gcacagataa tcaatgcatg tagtttatgc
    15961 gcagctcttt ctttttccaa tgccgccatg agatgcaaag ccaaataggc ccattcatct
    16021 tcattgatgg tatagttttt caaagccggc atgttgctga aatgcctttt tactgcgtca
    16081 aagactttgg gattttcttt ctttatctcc tcagtcaatg ggttctcaat gcgaatacct
    16141 cggctcaatc gaatcatcat cggtgacaaa tgatcgagta gtccattgaa aagctggaga
    16201 tcctctacta atgaaaaatg aaattcctga gacaatcttt tcagaacttt cgctaattcc
    16261 tttgatagtt cctgatcatt ttgattttct ttgtgattcg acttagccat gagatgtaat
    16321 gtcaaataag caatttcttc ttcgggaaac gatacacctg cgattacctc aatacgttga
    16381 acgatccgct gcgccacttg atactctact cgcttagaaa cttcttgttc gatgcctaat
    16441 tccttgattt caaacccttc tcggagccgt ttgatcccca aagccaagtg taaagtcaga
    16501 ttctgtatga taaaatccga aacttttaac ttagcttctc ttgtttcatc gagaatgata
    16561 atgactagct cttcaaaact gatgtctggg aaaaaacggc tttgccctaa atattctttc
    16621 aatgaatttc cataatggtt tccaaaaaaa gtatccatga taaaatgccg tcgatttctt
    16681 tcttcccctt cgatccatgt cccttgacca tgcttagaaa cgatttttag tgaatatgga
    16741 atcaatcgct ccttgatttc ttgcatgtcc tttgtcaaac tggaacgact gatataaagc
    16801 tcttctgcca aatcatccat aaaaagaatg gcatcttcca aaagcaattg gttcaatata
    16861 tacttttgtc gatcttcgga ctcataaaat ccttgcggtt ctctagcata tccaggtact
    16921 atttcttgcc gactcaaaaa caagtcaaat gtcagtcttt gtaaaatatg cagttggtag
    16981 ccataacctg gtttagcaat gatcttacct ccgttttttt ctactaactc tttgatccgc
    17041 tgcaagtaat tacggattgt ccgatcagat aataacaatt cagaagctaa acgttgactg
    17101 gtgacatact ccttcttgtg atcaatcaaa tatctcaaca gcaatttttc cttctgtttc
    17161 attatcatcc actcctattc catcatatgt tctcttcgat cacctctgct aacttctgga
    17221 tgcctgtagg gattgggaca tacgcttgga aagggatact aacaacaggc tttccaacag
    17281 attctcctaa ttttttgaat ttatccaaaa acatcgtcgt ttgcgggctg atcagaaata
    17341 aatcaaaatc actattctta atcatattat cgccttctgt agctgaaact gcatctacga
    17401 gtacctcttc tcttttttgc ttaaagaaat ccgttgtctt tgaggccatc atcgaggatg
    17461 acattcctgc cgcacaaata atcaatgctt ttttcataaa tcaatcactc cttttatttt
    17521 tataaaccaa gcatatcttg taagcgtttt ctattcaaaa tatatatttc cgtagcattg
    17581 cggaaaaaat gtgtatcata tcttgaataa taatttctgg caccgtgata tactagtgat
    17641 agaatttttt caggagggga tttagtgagt atcacagtga atattttgta tacaagtaca
    17701 aatggacaag ccagaaaatt tgcagaagaa atggtcgaaa gaaacattgt caaagcgatt
    17761 cgtgcagaag aaggaaacga gaaatatgac tattactttc cactagaaga tccagaatct
    17821 cttttactga tcgaccgttg gaaagatgaa gaagcaatcg aaaagcatca caaatccgaa
    17881 atgatggctc aaataaacga actccgtaaa aagtataaat taaaaatgaa agtcgaacgt
    17941 ttcactgatt taccgtaaaa agatacacat ggaatgatag caatttatca tccatgtgta
    18001 tcttcacttt ttccttattt tttattcgat ctcatctaat gattcaaacc gtgtttttct
    18061 cttatttttc ggataagtat ttcagtcacc ccaatataca tgattcctaa acatacccca
    18121 aggatattct caaaaatgcg gatcgttact gctcctggaa ccccgaataa actggcagtg
    18181 attgccaaag caccaaaaga attaaaaact gttttatatc tataggtaga acaaacacct
    18241 aaagctagac cacctaatat accgacccag ttaaaaggaa taaattggct gataccaata
    18301 aataaaacac atccaatcaa tgagccaatg attcggtcta ctgctcgttc tttgaaacca
    18361 atcgacatca atccatagct agatacaata gaagaaaatg caaaccctgc ccacatcaat
    18421 cgctgaaaag ggacatactc tcctgcaacg atcaaaagac taatgccaaa tgcatagatg
    18481 agcatccaaa ttttttcctg cgaatacatg cctttcaaaa aaatctcttt aaacaagctt
    18541 ttgtctttgt tcttttctcg atgttttctg taaaggagaa ctgaaaacca acaaaaaaaa
    18601 caaaaataat aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa
    18661 cgaataaaca ataaagagat atgaaaagcc gtacaaagaa gcattcccca ttttgggatc
    18721 actagcggtt gccattaata tgctgcttag taaaataaaa tgaatcaaca tgcccataac
    18781 tgaccatttg atcagctgga catacggtgc tactaatagt gagaacatga cgattgctaa
    18841 acctatgatc gaatgggaaa tcttataacc aaagtcaaca aaacgaatac tcatcaacat
    18901 acagaataaa accacagcgt atggtttatg cttcgattca aataatgttg taattataat
    18961 gataaaacaa atggcaaaca atacgatgaa taatgatcgt aaaaacaatg aacaaatata
    19021 catgcacttt tcttttttag aagctgcttc tcgtattttt tgttttaaaa taaacggatc
    19081 tagttgtaga gactgataaa aatccacctg ttgtcgtcct ttctttttac ttttattttt
    19141 tacagtcatc tatgacaggt tgtaaatact atggaacatc atatggcgtt gtcattttct
    19201 tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc ttgtatgatt
    19261 aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt tctatcaaaa
    19321 aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac gaaaccttac
    19381 agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt gtctgtagaa
    19441 attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa aacaagaaga
    19501 aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata cgcctaaaaa
    19561 agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt tattttatcc
    19621 gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta ctgactgaaa
    19681 aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc gttattcgca
    19741 cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg tcttgagtgt
    19801 tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt ttatttccgc
    19861 ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat tcctcgatta
    19921 acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt tacagacatg
    19981 aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt ggtgattgat
    20041 cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac gagttggttg
    20101 aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag agccattaaa
    20161 actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc aaagtattac
    20221 ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt
    20281 gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc acttctcaac
    20341 gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct actccgatac
    20401 taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg atgggtacta
    20461 gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat ttcgtttctt
    20521 tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata taaactggct
    20581 aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg gattgtttcc
    20641 tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga tcgccacttc
    20701 ttgttctttt tcgatcttga aagttactgg tgtcgcatcc aattcatacc ccgttggcgc
    20761 ttgagtttct accagctgat atgctcctgg tgctaagcca tc
//
LOCUS       DACCCM010000051        20783 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCCM010000051 DACCCM010000000
VERSION     DACCCM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.054"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(19..1575)
                     /gene="rny"
                     /locus_tag="IVK78_001826"
     CDS             complement(19..1575)
                     /gene="rny"
                     /locus_tag="IVK78_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP6375298.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1986..3035)
                     /gene="recA"
                     /locus_tag="IVK78_001827"
     CDS             complement(1986..3035)
                     /gene="recA"
                     /locus_tag="IVK78_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP6375299.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3144..4385)
                     /locus_tag="IVK78_001828"
     CDS             complement(3144..4385)
                     /locus_tag="IVK78_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP6375300.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4625..5203)
                     /gene="pgsA"
                     /locus_tag="IVK78_001829"
     CDS             complement(4625..5203)
                     /gene="pgsA"
                     /locus_tag="IVK78_001829"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP6375301.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5412..6296)
                     /locus_tag="IVK78_001830"
     CDS             complement(5412..6296)
                     /locus_tag="IVK78_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6375302.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(7074..8360)
                     /locus_tag="IVK78_001831"
     CDS             complement(7074..8360)
                     /locus_tag="IVK78_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6375303.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8353..9621)
                     /locus_tag="IVK78_001832"
     CDS             complement(8353..9621)
                     /locus_tag="IVK78_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6375304.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9873..10334
                     /gene="mscL"
                     /locus_tag="IVK78_001833"
     CDS             9873..10334
                     /gene="mscL"
                     /locus_tag="IVK78_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP6375305.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10393..10839)
                     /locus_tag="IVK78_001834"
     CDS             complement(10393..10839)
                     /locus_tag="IVK78_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6375306.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10932..11177)
                     /locus_tag="IVK78_001835"
     CDS             complement(10932..11177)
                     /locus_tag="IVK78_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375307.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11291..11869)
                     /locus_tag="IVK78_001836"
     CDS             complement(11291..11869)
                     /locus_tag="IVK78_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6375308.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(11946..12476)
                     /locus_tag="IVK78_001837"
     CDS             complement(11946..12476)
                     /locus_tag="IVK78_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP6375309.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12480..13340)
                     /gene="rlmB"
                     /locus_tag="IVK78_001838"
     CDS             complement(12480..13340)
                     /gene="rlmB"
                     /locus_tag="IVK78_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP6375310.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13333..13728)
                     /locus_tag="IVK78_001839"
     CDS             complement(13333..13728)
                     /locus_tag="IVK78_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6375311.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13725..15137)
                     /locus_tag="IVK78_001840"
     CDS             complement(13725..15137)
                     /locus_tag="IVK78_001840"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP6375312.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15134..15670)
                     /gene="cysE"
                     /locus_tag="IVK78_001841"
     CDS             complement(15134..15670)
                     /gene="cysE"
                     /locus_tag="IVK78_001841"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP6375313.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(15992..17452)
                     /locus_tag="IVK78_001842"
     CDS             complement(15992..17452)
                     /locus_tag="IVK78_001842"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP6375314.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17622..18779)
                     /locus_tag="IVK78_001843"
     CDS             complement(17622..18779)
                     /locus_tag="IVK78_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP6375315.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18813..20183)
                     /gene="radA"
                     /locus_tag="IVK78_001844"
     CDS             complement(18813..20183)
                     /gene="radA"
                     /locus_tag="IVK78_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP6375316.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20246..20773)
                     /locus_tag="IVK78_001845"
     CDS             complement(20246..20773)
                     /locus_tag="IVK78_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP6375317.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         5473 a         4593 c         3706 g         7011 t
ORIGIN      
        1 cttcttttgt attttttatt atttagcata atcagttgca cgagtttccc gaataacgat
       61 tactttgatg tgtccaggat aatccagttc ttcttcgatt cgtttacgaa tatcacgtac
      121 taaacgaaca gcgtctaaat ccgagatttc ttctggcttg accattaccc gaacttcgcg
      181 tccagcttga actgcgtaac ttgattctac accggcgaag ctgttcgaaa tattttccag
      241 acttcttaaa cgacggatgt agttttctaa tgattcactt cgcgcaccag gtcttgctgc
      301 tgagagcgca tctgctgcag ctacaaggac agagataaca gaagtagctt ccacatctcc
      361 atgatgagaa gcgatcgcgt tgattactac tgggttttct ttgtacttgg cagctaattc
      421 cgcaccgatc tcaacgtgag atccttcaat ctcatggtca agtgctttcc caatatcatg
      481 gagcaacccg gcacgttttg ctaattggat atcttcgcca agctctgctg ctaagatacc
      541 agctaatttt gcaacctcga ccgaatgctt caagacgttt tgtccataac tagttctgaa
      601 tcgcagacgt ccgatgatct tgattagatc tggatgaagt gtatgagcac cgacttcaaa
      661 cgctgcctct tccccgtatt cgcgaatacg ttcatccatt tctttacgag atttctcgac
      721 catttcttcg atacgcgctg gatggattcg tccatcttgt atcagttttt ccaaggtcat
      781 ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga acaactgctt ccggtgtatc
      841 atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg
      901 tccaatgata cgacctttca tttcatcatt cggaagggta acgactgaaa cagtcgtttc
      961 tgaaacttga tctgctgcgc agcgttgaat cgctaatgat aataagtttt tcgctttgcg
     1021 atctgcttct tctttggcac gctgttcaga ttcttttacc attagagtta actcatgatt
     1081 cagctcttct tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg ataaaccagc
     1141 aattttttct agttcttttt gttgatcttc aatcattttt tcaacgtctt tttctcgctc
     1201 ttcaattaat tgctgttttg aactaagtgc ttcttcttta ctttcaagtg agttctcgcg
     1261 tttttctaac gagtcatctt tacgattcag cgtttgttcg cgttgaatca aacgattctc
     1321 ttgagatttt aattctaact tactttctct cagctcactt tcaacttcag aacggtattt
     1381 ctgattttct tccttagctt ctaacaatgc ttcttttttt agagtttctg cttctttttt
     1441 tgcactatcg atgattccag cagctgatga ttgtgcacca gctatttctt tctcgtgacg
     1501 tgactttgcg accataaacc ctaaaccaag accgacaatt aaaccgatga tagcgaggag
     1561 aatgttgaat accataaaat ccacctccat actatctttt tcatttttat catctataca
     1621 gcttctatga taaattattc taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa
     1681 aaacatgcac aactttattc taatgtttgt accccgtagt gtcaacttta aataaacaaa
     1741 gaaacggctt tctacttatt cattaaacga tggtttttaa aataaatgac actagtttca
     1801 tataggtaaa cctttaaatt gagaaacttt tctattaaat tattctttgt agatgaacag
     1861 atatttttat tttatgtacc cactcgtata attaaataac tcacgatttc tttattcaat
     1921 aaaaaaacgg caggtcaagg agagtaccta ctcttcagac cagccgtttt tgtttatcat
     1981 aatttttatt cttctactaa aggcaattct tcttgtcctt tttcatcttc tgggacagcg
     2041 acttcttcac caataccata agctgctcta actagagcag aaacttctgc catcatttca
     2101 ggatgattag ccatatagat tttagcgttt tcccgtcctt gaccaatccg atcttcttta
     2161 taagaatacc aagcaccgct tttatcaaca atgtcttttt ctactgccat gtctagaagt
     2221 tctccttctt gtgagatccc taaaccatac atgacatcta cttcagcgat tttgaaagga
     2281 ggtgctactt tgttttttac gactttgatc tttgtacggt taccaacaat atctgtacct
     2341 tgtttcaatt gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg
     2401 cgtcctccag gagtgatttc tggattccca aacatcacgc ctactttttc acggatttgg
     2461 ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac cagagagttt acgcaatgct
     2521 tgagacatca aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc gtcgatttct
     2581 gctcgtggaa ctaaagcagc aactgaatcg actactacga tatctacagc cccacttgat
     2641 actaaagcat cagcgatctc tagaccttgt tctcctgtgt caggctgtga aagaagtagt
     2701 tcatcgatat tcacacctaa tttttgtgca tattgcggat ctaacgcatg ctcagcatca
     2761 atgaaagcgg ccgttccgcc atttttttgt acttctgcaa tagcgtgtag tgcaactgtt
     2821 gttttacctg aactttcagg tccatatact tcgatgatac gtccacgggg gtaaccgccg
     2881 acacctaaag cgacatctaa cgcaagagag ccacttggaa ttgtagagat ttgttggtcg
     2941 attttttcgc ccaatttcat aatagagcct ttaccatagc tcttttcaat ttttttcagt
     3001 gcagcatcta aggctgcttt acgatcatct gccaatacga taatcctcct tatatttaca
     3061 aatcagagca acatttcatt atccttatca tatctttttt agtgaaaaaa agcaagaaaa
     3121 aaccgaacaa atattcgcat ttatcatttt cttaaaagct gttttctcaa aagatcaaat
     3181 ccttgcataa ccgcattata tctgatagca gcacgatctc tggcaaagtg atatttttcc
     3241 gaaaccacgc cgtctttaga tgaaatagcg atccatactg ttcctgcaga ttgaccttct
     3301 aacgtgtccg gtccagccac tccggtgaaa gctacactgt aatctgttct tgctatctca
     3361 cgtgcttgaa tggccatctg ctccacacat tctttgctga ctgttccata ttctgctaat
     3421 agctccggat caatctccag aaaaccagct tttgtttgga gcgagtaggt aacgaatccg
     3481 cctggaaata cttcagatac accagcaatg tcgcctaatg cggcttggaa ggcgccggct
     3541 gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt tcagtagttc cactacgact
     3601 tttgctagcg aattatcatc gccgtatcca taaaaatatt cccctactcg ttcttggatt
     3661 ttttcttcta atgctaaaag agcttgattc cctgcctgaa catcatttgt tttcactgtc
     3721 agtcgtaatg tcacttcatt tggtttggca tatggtgcga tcgttggatt gatctgtgtc
     3781 tcaatcagat cttttagctc agtgaccagt cgcgattcac caatcccata aaagcgcaaa
     3841 actttagaaa ttagtttttc ttcagaagga aaacgttcct ctagtaaagg acgtacttgt
     3901 tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa gaaggatata cgcatgtgta
     3961 tctgtttgat aaaaagtccc taaagcaagc cctgtacgat ttggtagagg aaccccgcct
     4021 tcgataatct gggactgttg cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa
     4081 tatgccaata attttttgta tccttccgta ttttgtatga gagacttacc gaggtgagcc
     4141 gcagttgctt cttttgtcag gtcatcttct gtcggtccta atccgccaca taagagtatg
     4201 agttcacttc ttgtttcagc tagagcaatc aactcttcta aacgctgctg attatctcct
     4261 acaactgttt gataataaac ttcgatacct agatcagcta gctgttctga caagaaagtg
     4321 gcattcgtat tcacgacttg tcctagtaaa agttctgttc cgacagctat tatttctgct
     4381 ttcattactt cccgctccct tatatagtat acgctgtatt tttatatttt tttatttaaa
     4441 aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa ataaacagtg
     4501 ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa aagctgtttt
     4561 ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct ccactaaacg
     4621 atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc acacctgaat
     4681 agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata ccgatcaggc
     4741 tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta gccgtcttga
     4801 cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt aaacgcaagc
     4861 ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg gcctttcctt
     4921 ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac gggtccgcaa
     4981 acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct aaccagtctg
     5041 tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc agtgtggtgt
     5101 ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg aaaatcggga
     5161 tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg actcctttct
     5221 tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt ttccaaatga
     5281 atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga agaaggaccg
     5341 cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga tgcgtaaact
     5401 aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg actctgattg
     5461 ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc attgcttgct
     5521 gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact ctgtgtttca
     5581 tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc taaccataca
     5641 cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat atttactgcg
     5701 atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc acttgatttt
     5761 gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga ggaagaggac
     5821 tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat tggatcagct
     5881 tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc aaccaatcct
     5941 aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc ctgatgcatt
     6001 gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga tcctgtcaat
     6061 ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac agcttcagcg
     6121 tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa atcatcattg
     6181 tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc atctaaagat
     6241 atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa agccactcgt
     6301 tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga aagacgttat
     6361 gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa acattgttct
     6421 cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc tgttttttga
     6481 acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct taaacggtgt
     6541 tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga gatatttttc
     6601 cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc cttaaacggt
     6661 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt aagaagctgt
     6721 ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttggtaca
     6781 cggtgttcca gaagtagctt cttcggcaat aagccgaaat atcacaaaaa tttgagaagc
     6841 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctctttga
     6901 ttacttcgtt tgattttatt ttaatataag ttgattaaaa gagagcatga tttgttttat
     6961 tttctcacta atttttccaa cacaactaag aataccataa aagctgattt tggaggaggt
     7021 tcctactaca agtctaaaac gaaatttaag acttttcctc ataaaatact ggtttatttt
     7081 ggtctcatgt aaaactcgct gaaggcctct tcacttataa atgcttcccc agctgccagt
     7141 acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt tcgatcgcca
     7201 aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga ttggaaatat
     7261 tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc cggatcatca
     7321 gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc gtccgtatct
     7381 ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc aaagctgtca
     7441 tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc taaaatcatt
     7501 tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc ttgcggcaat
     7561 gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc ttccaatgca
     7621 ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc ttgctttgga
     7681 gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc aggctccatc
     7741 ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata gcacgtatac
     7801 aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc gatatgcaat
     7861 gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt agggtcgtct
     7921 tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt ttcttctgtg
     7981 aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac ctgatccgtc
     8041 gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga agctccttgt
     8101 ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg ttctaaaaaa
     8161 tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat aaattcgtta
     8221 tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc atttttaggc
     8281 aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc attgatttgc
     8341 tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg cctgcaattc
     8401 gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac gtttcaacca
     8461 ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag cttcgattgc
     8521 cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt ttgtctgaga
     8581 cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt gaatcagatg
     8641 aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga atcctcggaa
     8701 cgtatccacg ctactagagg cataataagc tagactttct ttctctctga cattcatgaa
     8761 aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg caaaacgttc
     8821 tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt gaacgattgg
     8881 ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa acagatctgg
     8941 ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg ctttgtccgg
     9001 atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt cttgatagta
     9061 ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca accctgggat
     9121 cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag caaaagtttg
     9181 tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca agtcaaacgt
     9241 caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt ttaaaaaatc
     9301 taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa aactgatccc
     9361 tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc ttgcgccata
     9421 aagctctgca agttgcgcac ttaactttgt ctgatccgga taattcaaac tatttgtttc
     9481 caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac gcgcagtaaa
     9541 acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa gattcacgcc
     9601 ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg cccgcttact
     9661 cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt ataagttcag
     9721 gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag atgtttttta
     9781 tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt tttcgatata
     9841 ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa gagttcatca
     9901 tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg tttacaagca
     9961 tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt gtcgcaatga
    10021 tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg ccgacaaaag
    10081 gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg atcactggtt
    10141 ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc tccaaagaag
    10201 agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac atacgcgatc
    10261 ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca aatctttccg
    10321 atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg tcggctttct
    10381 ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg gaatatcatc
    10441 ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag cttgtaggta
    10501 ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca tgttaatcgt
    10561 ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa caaggacacg
    10621 tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga tcttcccatc
    10681 agtatcttga aaaaacgtat gccaagcttg tccatcaatg tcatcaactt cttgataagg
    10741 acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt aaaactctgc
    10801 tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc cccctgcttt
    10861 tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg aatctcaggc
    10921 tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg agtaagtaca
    10981 tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa atcaacaacg
    11041 aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg ctttcttcct
    11101 gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt cttgattgaa
    11161 gctaaagttg ttggcataca gttcacctct actatcaact ataccacata tttttaaaat
    11221 tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga
    11281 tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc
    11341 tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa
    11401 aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt
    11461 cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt tcttgcaatg
    11521 tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat
    11581 ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc
    11641 ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca
    11701 atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta
    11761 aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa
    11821 atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc
    11881 tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg
    11941 atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc
    12001 tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg
    12061 gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat
    12121 gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt
    12181 gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct
    12241 tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact
    12301 agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa
    12361 cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt
    12421 tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc
    12481 tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca
    12541 tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc
    12601 aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca
    12661 ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct
    12721 ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact
    12781 gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg
    12841 atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga
    12901 tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca
    12961 atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc
    13021 acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct
    13081 tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga
    13141 aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg
    13201 aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc
    13261 tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg
    13321 tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc
    13381 ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt
    13441 tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct
    13501 tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg
    13561 aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt
    13621 gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc
    13681 tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc
    13741 catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga
    13801 atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat
    13861 tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc
    13921 cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct
    13981 agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta
    14041 aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta
    14101 tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac
    14161 gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg
    14221 atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt
    14281 ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct
    14341 tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc
    14401 gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca
    14461 tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa
    14521 ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc
    14581 cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt
    14641 gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta
    14701 cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact
    14761 tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggc
    14821 tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct
    14881 ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa
    14941 ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa
    15001 gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca
    15061 tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta
    15121 ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg
    15181 ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac
    15241 cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca
    15301 tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg
    15361 ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc
    15421 ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac
    15481 cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat
    15541 ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga
    15601 tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa
    15661 accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat
    15721 aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc
    15781 cacttttctt cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc
    15841 tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca
    15901 gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga
    15961 tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta
    16021 atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc
    16081 ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt
    16141 gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct
    16201 gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg
    16261 cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag
    16321 catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt
    16381 catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa
    16441 atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat
    16501 cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt
    16561 gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca
    16621 ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt
    16681 cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg
    16741 ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat
    16801 ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa
    16861 aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg atattatcgg
    16921 attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca
    16981 cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg
    17041 tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc
    17101 gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca
    17161 gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt
    17221 ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact
    17281 ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa
    17341 agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa
    17401 tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc
    17461 tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa
    17521 tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc
    17581 caactggttg aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc
    17641 tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag
    17701 atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc
    17761 atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct
    17821 tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg
    17881 actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca
    17941 gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat
    18001 ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca
    18061 gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca
    18121 cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg
    18181 atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg
    18241 atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac
    18301 agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa
    18361 actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg
    18421 atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg
    18481 gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga
    18541 taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt
    18601 ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat
    18661 gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat
    18721 gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc
    18781 gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca
    18841 gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg
    18901 tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca
    18961 atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt
    19021 ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc
    19081 gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga
    19141 agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga
    19201 cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct
    19261 accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct
    19321 aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc
    19381 atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc
    19441 cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac
    19501 attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc
    19561 ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg gctgacactt
    19621 cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa
    19681 tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga
    19741 taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt
    19801 tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt
    19861 aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc
    19921 accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct
    19981 tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc
    20041 cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg
    20101 caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat
    20161 tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa
    20221 atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt
    20281 gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac
    20341 gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat
    20401 ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca
    20461 acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt
    20521 ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt
    20581 ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt
    20641 tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat
    20701 gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc
    20761 ctctttttct caccggtttt cct
//
LOCUS       DACCCM010000052        20746 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCCM010000052 DACCCM010000000
VERSION     DACCCM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.055"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            168..362
                     /locus_tag="IVK78_001846"
     CDS             168..362
                     /locus_tag="IVK78_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375318.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..929
                     /locus_tag="IVK78_001847"
                     /pseudo
     CDS             631..929
                     /locus_tag="IVK78_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1026..1460)
                     /locus_tag="IVK78_001848"
     CDS             complement(1026..1460)
                     /locus_tag="IVK78_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6375319.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="IVK78_001849"
     CDS             complement(1692..2402)
                     /locus_tag="IVK78_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6375320.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="IVK78_001850"
     CDS             complement(2392..3252)
                     /locus_tag="IVK78_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP6375321.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="IVK78_001851"
     CDS             complement(3257..3895)
                     /locus_tag="IVK78_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP6375322.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="IVK78_001852"
     CDS             complement(3910..4209)
                     /locus_tag="IVK78_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6375323.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="IVK78_001853"
     CDS             complement(4252..5736)
                     /locus_tag="IVK78_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6375324.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="IVK78_001854"
     CDS             complement(5756..6217)
                     /locus_tag="IVK78_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6375325.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IVK78_001855"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IVK78_001855"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP6375326.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="IVK78_001856"
     CDS             7520..8290
                     /locus_tag="IVK78_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6375327.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="IVK78_001857"
     CDS             complement(8563..9390)
                     /locus_tag="IVK78_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6375328.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="IVK78_001858"
     CDS             9958..11193
                     /locus_tag="IVK78_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6375329.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="IVK78_001859"
     CDS             complement(12192..12452)
                     /locus_tag="IVK78_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6375330.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="IVK78_001860"
     CDS             complement(12479..13780)
                     /locus_tag="IVK78_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6375331.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFAVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="IVK78_001861"
     CDS             complement(14072..14530)
                     /locus_tag="IVK78_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6375332.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15868)
                     /locus_tag="IVK78_001862"
     CDS             complement(14543..15868)
                     /locus_tag="IVK78_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP6375333.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17297)
                     /locus_tag="IVK78_001863"
     CDS             complement(15885..17297)
                     /locus_tag="IVK78_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6375334.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="IVK78_001864"
     CDS             complement(17314..18078)
                     /locus_tag="IVK78_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP6375335.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="IVK78_001865"
     CDS             complement(18182..19057)
                     /locus_tag="IVK78_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6375336.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="IVK78_001866"
     tRNA            complement(19312..19396)
                     /locus_tag="IVK78_001866"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="IVK78_001867"
     tRNA            complement(19414..19485)
                     /locus_tag="IVK78_001867"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="IVK78_001868"
     tRNA            complement(19495..19567)
                     /locus_tag="IVK78_001868"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="IVK78_001869"
     tRNA            complement(19629..19713)
                     /locus_tag="IVK78_001869"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="IVK78_001870"
     tRNA            complement(19734..19804)
                     /locus_tag="IVK78_001870"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
     gene            complement(19850..19921)
                     /locus_tag="IVK78_001871"
     tRNA            complement(19850..19921)
                     /locus_tag="IVK78_001871"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19887..19889),aa:Gln,seq:ttg)
     gene            complement(19933..20005)
                     /locus_tag="IVK78_001872"
     tRNA            complement(19933..20005)
                     /locus_tag="IVK78_001872"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:His,seq:gtg)
     gene            complement(20008..20081)
                     /locus_tag="IVK78_001873"
     tRNA            complement(20008..20081)
                     /locus_tag="IVK78_001873"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20047..20049),aa:Trp,seq:cca)
     gene            complement(20088..20168)
                     /locus_tag="IVK78_001874"
     tRNA            complement(20088..20168)
                     /locus_tag="IVK78_001874"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20132..20134),aa:Tyr,seq:gta)
     gene            complement(20170..20245)
                     /locus_tag="IVK78_001875"
     tRNA            complement(20170..20245)
                     /locus_tag="IVK78_001875"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20210..20212),aa:Phe,seq:gaa)
     gene            complement(20281..20353)
                     /locus_tag="IVK78_001876"
     tRNA            complement(20281..20353)
                     /locus_tag="IVK78_001876"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Asp,seq:gtc)
     gene            complement(20363..20435)
                     /locus_tag="IVK78_001877"
     tRNA            complement(20363..20435)
                     /locus_tag="IVK78_001877"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20400..20402),aa:Val,seq:tac)
     gene            complement(20451..20522)
                     /locus_tag="IVK78_001878"
     tRNA            complement(20451..20522)
                     /locus_tag="IVK78_001878"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20487..20489),aa:Glu,seq:ttc)
     gene            complement(20537..20626)
                     /locus_tag="IVK78_001879"
     tRNA            complement(20537..20626)
                     /locus_tag="IVK78_001879"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20590..20592),aa:Ser,seq:gga)
     gene            complement(20666..20739)
                     /locus_tag="IVK78_001880"
     tRNA            complement(20666..20739)
                     /locus_tag="IVK78_001880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20703..20705),aa:Asn,seq:gtt)
BASE COUNT         6557 a         4343 c         3291 g         6555 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atataaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagcaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcgtcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaaa ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
    19261 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
    19861 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
    19921 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
    19981 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
    20041 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
    20101 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
    20161 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
    20221 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
    20281 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
    20341 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
    20401 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
    20461 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
    20521 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
    20581 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
    20641 tcaatactct ttctcaaaat gagaactccg gcagtaggac tcgaacctac gacatcatga
    20701 ttaacagtca tgcgctacta ccaactgagc tatgccggaa taacat
//
LOCUS       DACCCM010000053        20730 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCCM010000053 DACCCM010000000
VERSION     DACCCM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.056"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK78_001881"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK78_001881"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP6375337.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IVK78_001882"
     CDS             complement(2261..2512)
                     /locus_tag="IVK78_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP6375338.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IVK78_001883"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IVK78_001883"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP6375339.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IVK78_001884"
     CDS             3462..3680
                     /locus_tag="IVK78_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375340.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="IVK78_001885"
     CDS             complement(3969..4481)
                     /locus_tag="IVK78_001885"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP6375341.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IVK78_001886"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IVK78_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP6375342.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPTGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7368)
                     /locus_tag="IVK78_001887"
     CDS             complement(6934..7368)
                     /locus_tag="IVK78_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP6375343.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="IVK78_001888"
     CDS             complement(7378..8166)
                     /locus_tag="IVK78_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6375344.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="IVK78_001889"
     CDS             complement(8163..9104)
                     /locus_tag="IVK78_001889"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP6375345.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="IVK78_001890"
     CDS             complement(9281..9868)
                     /locus_tag="IVK78_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP6375346.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IVK78_001891"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IVK78_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP6375347.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="IVK78_001892"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="IVK78_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6375348.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="IVK78_001893"
     CDS             complement(12785..13720)
                     /locus_tag="IVK78_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6375349.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13906..14865
                     /locus_tag="IVK78_001894"
     CDS             13906..14865
                     /locus_tag="IVK78_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP6375350.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14951..16045)
                     /locus_tag="IVK78_001895"
     CDS             complement(14951..16045)
                     /locus_tag="IVK78_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6375351.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16234..16995
                     /locus_tag="IVK78_001896"
     CDS             16234..16995
                     /locus_tag="IVK78_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6375352.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17110..17550)
                     /gene="fabZ"
                     /locus_tag="IVK78_001897"
     CDS             complement(17110..17550)
                     /gene="fabZ"
                     /locus_tag="IVK78_001897"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6375353.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17770..18525
                     /gene="fabI"
                     /locus_tag="IVK78_001898"
     CDS             17770..18525
                     /gene="fabI"
                     /locus_tag="IVK78_001898"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP6375354.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18635..19648)
                     /locus_tag="IVK78_001899"
     CDS             complement(18635..19648)
                     /locus_tag="IVK78_001899"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP6375355.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19813..>20730
                     /locus_tag="IVK78_001900"
     CDS             19813..>20730
                     /locus_tag="IVK78_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP6375356.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         5972 a         4455 c         3671 g         6632 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgttggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataaccc gcgaattgta aaattaagtt agaaaaataa aaaggcattt atggtacact
    13861 caaattgtat ttccgacgaa agaaaacaaa ggagtgtaac cataaatgac ctacaaacat
    13921 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
    13981 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
    14041 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
    14101 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
    14161 cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg
    14221 agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa
    14281 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
    14341 atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt
    14401 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct
    14461 aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt
    14521 aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga
    14581 aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg
    14641 gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat tctgcgtcgt
    14701 aatggactgc cgaaatcaat ggattttaga aaagtgaatc agacatttat ttccagtgtc
    14761 agcaatcaac gtaatcatat tccaagaaaa tcattgaatt acagaacacc aatcgaaata
    14821 tttttgagct atgtacaaga agcattttat tctagcttaa tttgacaaat cataacctat
    14881 aaaaaaacaa aataaaaaag cagaaataac tggctatttc tagcggatta tttctgcaat
    14941 aactaattat ctatttttta ttttcaatta atttaaagct tgaccatggt ttcaaaaagt
    15001 caaggaggaa taattcgtca tcggagattc ttcctacaac attgacacgt ccgtcgtttt
    15061 tcatatcctt caaggcgatt tgtgtttctc ctttgtattg accgtaccct tcattgtcaa
    15121 tcagtacatc cccatgatgc atatctcgcg tattatgagg cgggaaatct ttatctttgt
    15181 agtatacacg agtcatcgtt gaacgaagga tatattctga gcgatcccct cgataactat
    15241 gaaggttatc aaacaagcag attcgttcgt tttctgtaat accttcttcc gtatctactt
    15301 tcaatgttgg ataatccgcg ttaaatgctt ctgccatttc tttcaattct tcttcacttg
    15361 cgtacgcatt tccaatagaa atatcatcaa tcaatcctgt aaggattaaa tgcttcactt
    15421 gtgtcgcaat ttcaagatct cggtggtctt ccagcgtgca caagccatct tgcgtaggcc
    15481 aagggccaaa ctcagcagat tgcgaattga cgaatgccat cgtattcaaa ttatattttc
    15541 tgaatttttc tgaacaaaaa acaaagtgtt cgtatcctaa accagagtat cgatgaggat
    15601 aaaaattatg agaacctaat aaattatccg tgtttggcga ataactcatg atgttgtcaa
    15661 cgtaattcgt gcccgaactc atattgatct cgattttgat accaaaagga ttgcgcgtca
    15721 tttttgcttc ttctgctcct gtgaatccga tatctaagcg gaccccgtag gcacccatct
    15781 tatggaaaaa agataagtca tcgtaagaaa tctccaactg ttcaaataac gctggattga
    15841 tatcaaccat tacttccatg cctaggctat tcgcgtaatc aactacttct ttaaattctg
    15901 aaagaacttt ttctttatca tcattgatct gcagcaaact ggtaaaaacc cgtttatacc
    15961 catacttatg agctaaatcc aagtacgctt tgtccttttc aaatgttgac cgttctggat
    16021 aaatcgagat tcctaatttt cccatattac catttcctcc tgcatctttt cgtttttgta
    16081 tccacttaca ttatagcaaa cacaaagtaa atggtctaca ccttttcttt ttcttttaaa
    16141 ataaatgata aattccaata atttaagtag gtattttccc tctattttgt tatcattaaa
    16201 gataacaaaa tagtaaattg gaaaggaaca gttatgaaga aaaataaagt tattttaagt
    16261 attagtttag cagcagcatt gctgtttgct gctggttgtt ctgcaaacaa cacgacttct
    16321 tctagttcgt ctacagaaag ttcaaaaagt acatctactg cggaaagcac gactgccagt
    16381 agcgtcgatt tgaacgcatt ggaattacca caattgacga aagatgtcgc cgaaaatgaa
    16441 gatctggtcg aaatggtgac gaataaaggc acaatcaaaa tcaaattatt tcccgaacac
    16501 gcaccaaaag cggttgaaaa ctttatgaca catgcaaaag acggttacta caacggactg
    16561 actttccatc gcgtcatcaa agatttcatg atccaaggtg gcgatcctaa aggcgatggc
    16621 actggagggg aaagcatttg gggagaagga tttgaaacag agatcaacaa ccacctttat
    16681 aatataagag gtgcgttatc tatggctcgc tcacaagatc ctaacagtaa cggcagccag
    16741 ttctttatcg ttcaaaatac agatgatatg catgatggtc ttttaaaaga cgactatcct
    16801 caagcaatca tcgacgctta taaaaacggc ggttatcctt cactagatgg ccattatact
    16861 gtcttcggcc aagtgattga aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca
    16921 gatagttcag acaaaccaaa agaagaagta aaaatcgata aaatcaatat actgcaagaa
    16981 gcaaaacaaa aataaaaaaa taacgtctga atcctgcata gatgacaacg tatgctagga
    17041 ttcaggcgtc ttctttttac ataaaaagca agtaagcttc cttttgtttt gctatatcag
    17101 gttttatagt caagcatttt ggttgacgat gaacgtgaac tcgcactcac agactttttt
    17161 atcatctaca tatgctgcag ataacgctgt accgactgat cccttcattt tggtgatttc
    17221 aaaatgacat ttcagcacat ctcctggaac aactttttga cggaatttgg ccttattgat
    17281 cccaccgata taagctcttt ttcctagaaa ctcttctgat tttaggataa gtatcgaacc
    17341 tgcttgtgcc agtgtttcta gaatcaacgc acccggcatc actggattgt caggaaagtg
    17401 accttggaaa aaatcttcat tgattgttac attttttgtc gcaacgatct tttttcctgg
    17461 ttctaaagta tctacataat caatataaca aataggataa cgattaggaa tcaattccat
    17521 tacttcagta gcagataata cgtaagacat aattaaactc cttctttcag gcaattgcca
    17581 ttttcattac tttgactatc aaagtatcat attcctaaat atttagcaat ctttttttaa
    17641 tttaagaaaa aatagtttga caatcaaaac attttgacta ataattaaga aatcaagaag
    17701 ttattttagc tattattctc tattttttta cgactatcaa atttttatca gaaacaaagg
    17761 agagtcatta tgtcatttct agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa
    17821 aaaagcattg cttggggttg tgctgaagca atgttgaaac aaggggcaga aattatctat
    17881 acttaccaaa atgaacgaat gaaaaaatca ttagtcaaat tggtcggcga agaagcattc
    17941 actgtagaat gtgatgtttc tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt
    18001 gaatatgctg gggagatcca cggccttgtc catgcagttg cttacgccaa caaagaagaa
    18061 ttgtccggaa atgtgtctga tatttccaga gacggctatc aattagctca agatatcagc
    18121 agctactctt taattgctgt atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg
    18181 atcgttagca tgacttatct aggttcagaa cgcgcaatcc cgaattataa catgatgggt
    18241 attgcgaaag cttctcttga agctgctatc cgttatttgg cagctgagtt ctcgcctaaa
    18301 ggaattcggg tcaatggaat ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt
    18361 aaagactatc aaaaactgat ccagttatct gaatctcgta cgcctgatca gcaaggtgtg
    18421 acgatcgaag aagtcggtaa tgcttgtgct ttcttaatca gcccactttc cagcggaatc
    18481 atcggtgata ttatctacgt tgacaaaggt gtccatcttt cataaacttt tgttgcattc
    18541 tttaaagtaa aacatataaa aatcaggaaa aaacttttta aagtctcttc ctgatttttt
    18601 ctttttacgt ttttttcaac tcattacttt gaaattattc cacgacttca atccagcctt
    18661 ctggtgcttc tatgtctcca aattgaatac ctgtcaattc atcatacaac cgttttgtta
    18721 ctggtcctac ttccgtttca ctataaaata catgaagatt gttattgtga taaatcccac
    18781 cgattggaga aataacagcc gctgtaccac atgcaccagc ttctgcaaat tgatctagat
    18841 catcaatgaa aatatctcct tcaattgctt gcagtcctag tcgttcttca gctaactgca
    18901 acaaggaata tttcgtgata cttggtaaga tagagggcga ttgcggagtg atgaattttc
    18961 cgtcttttgt aattccaaag aaatttgctg cacctacttc ttcgattttc gtatgtgtgg
    19021 ctggatcaag ataaacacaa tctgagtaat ccagttcttt tgcttctgtt cctggaagta
    19081 aactcgctgc gtaatttccg ccgacttttg ctgcaccagt ccccttagga gcagcacgat
    19141 cgtaatcaga tacaataaaa ttcgttggtt ttagtccacc tttgaaatac gctcctactg
    19201 gcgtacagaa gacgacaaat aaatattctg gagctggatg gacaccaatg ttttcaccga
    19261 ccccaatcag taaagggcgc aagtataatg tagcgcctgt tccatagggc ggcacaaaat
    19321 cttcatttgc ttttaccact tgcttgacag catcaacgaa catcttttca gggaccattg
    19381 gcatcagcaa tcgtctagca ctttgattca tccgtcgact attttgttca ggtcgaaaga
    19441 gattgacctt gccgtctttt cgacgataag cttttagacc ttcaaaacat tgctgaccat
    19501 aatgaagcgc tggagagcct tcatgaatcg tcaggtaatt atcttctgtc aattctcctt
    19561 cttcccattg tccgtctttc catttggcaa taaaacgcca aggagttttg atataagaaa
    19621 aacctagatt gttccaatcg atttgcatat acttaccacc ttcgttattt tctccacatt
    19681 ttatcattat ccatattatt tgtcatttta ttttcagctt ttttccttca ttatagttat
    19741 ttttgctatt tccttttttg aaaacttctt ggtacaatac agaaagaatc aaattttaga
    19801 aaagaggatt ttatgttttc attattagca actgccggta cgacagactc ttctggcgag
    19861 acattgacag atcaagctgt cagcaaagta aatgcgtttc agcggttttg ggacgggatt
    19921 gattgggacg ccattttggc tgcctttatc caaaaaagtt tatatctgat ttttttgatc
    19981 gtcctttttt ggattcttca ccgtgtggga aaatatctga ttgataagtc ttttaatcag
    20041 tattctaaaa aagccattct aaacgagagc cgcttaaaga ctttacatag tcttttaaat
    20101 actatttttc attataccct tggtttcttt tttatctatg caattttatc tgctatcggt
    20161 gtgccgatcg gatcactttt ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt
    20221 gcccaagggt tcatgagtga tgttattacc ggatttttta ttatcatgga acaacagatg
    20281 gatgttgggg actacatcaa gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt
    20341 ggtatccgta cccttcagct gaaagcaacg gatggtactg tccatttcat cccgaatcga
    20401 aacatcacca cgatcagcaa tctctctcgt gcaaatatgc aagtactttt agatattcgg
    20461 attgttcctg aagaaggata tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca
    20521 ttagctgaaa aataccaaga agagctccaa actgaacctt ctatctttgg attagtggat
    20581 cttggaaaca gcaattttgc gattcgtacg gtctgctacg cattgaacgg aaaacaatat
    20641 gccttaaaag aagagttctt gagctcctat gtcaaagaat tgacagcttc tggtttcacg
    20701 attccgaaca gtccaattgc tgtcggaaaa
//
LOCUS       DACCCM010000054        20281 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCCM010000054 DACCCM010000000
VERSION     DACCCM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.057"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            136..456
                     /locus_tag="IVK78_001901"
     CDS             136..456
                     /locus_tag="IVK78_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375357.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            908..1108
                     /locus_tag="IVK78_001902"
     CDS             908..1108
                     /locus_tag="IVK78_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6375358.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1255..4044
                     /locus_tag="IVK78_001903"
     CDS             1255..4044
                     /locus_tag="IVK78_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6375359.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4091..4618
                     /locus_tag="IVK78_001904"
     CDS             4091..4618
                     /locus_tag="IVK78_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6375360.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4639..5829
                     /locus_tag="IVK78_001905"
     CDS             4639..5829
                     /locus_tag="IVK78_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6375361.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5869..7167
                     /gene="asnS"
                     /locus_tag="IVK78_001906"
     CDS             5869..7167
                     /gene="asnS"
                     /locus_tag="IVK78_001906"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP6375362.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8220..8894
                     /locus_tag="IVK78_001907"
     CDS             8220..8894
                     /locus_tag="IVK78_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6375363.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8891..9232
                     /locus_tag="IVK78_001908"
     CDS             8891..9232
                     /locus_tag="IVK78_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375364.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9262..9621
                     /locus_tag="IVK78_001909"
     CDS             9262..9621
                     /locus_tag="IVK78_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP6375365.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9824..10003
                     /locus_tag="IVK78_001910"
                     /pseudo
     CDS             9824..10003
                     /locus_tag="IVK78_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10145..12226
                     /gene="cadA"
                     /locus_tag="IVK78_001911"
     CDS             10145..12226
                     /gene="cadA"
                     /locus_tag="IVK78_001911"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6375366.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12537..14003
                     /locus_tag="IVK78_001912"
     CDS             12537..14003
                     /locus_tag="IVK78_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6375367.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14618..15157
                     /locus_tag="IVK78_001913"
     CDS             14618..15157
                     /locus_tag="IVK78_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP6375368.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15231..15671
                     /locus_tag="IVK78_001914"
     CDS             15231..15671
                     /locus_tag="IVK78_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP6375369.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15684..15893
                     /gene="copZ"
                     /locus_tag="IVK78_001915"
     CDS             15684..15893
                     /gene="copZ"
                     /locus_tag="IVK78_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP6375370.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALYGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15893..18079
                     /locus_tag="IVK78_001916"
     CDS             15893..18079
                     /locus_tag="IVK78_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP6375371.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18099..20270
                     /gene="copB"
                     /locus_tag="IVK78_001917"
     CDS             18099..20270
                     /gene="copB"
                     /locus_tag="IVK78_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP6375372.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6773 a         3527 c         4406 g         5575 t
ORIGIN      
        1 agaggttgtg acgtggtcac gacctctttt tgacgattat gatacaaaaa acatcattta
       61 tgaacaaaat gtgaataacg ggcgttcttg agatatgatt ttaattggga aaaacttatc
      121 gataaggagg tctttatgaa aaaaagactg ttatggtgtt tgctgtttct atccctaagt
      181 ttaacaagtc agccagtttt tgctgaagaa acacagcggt ctcctattac aggcacacgt
      241 ctgtacaaaa atgtttatgt acagacaaca gatcttgatt ctgcgattcc tgaaacagaa
      301 gaacggtctt tgcagccaag atttggttac ttttggtact gtccgacctg tgattatgaa
      361 agttgctggc atttcttaca ttctacagct gcagcttttg cttttactca tactcagaaa
      421 tatgatggac atcatcctgt tatttatgag gtatagatat gagcaaaaaa gaaaaactaa
      481 tctcgttgat cgtattggtt tgccttgttc ttctggtaac tgtcataggc tatcagaaat
      541 accatgagaa catcagacct actaaagaat cttccccagc ctcgaccgtt gaaacagaac
      601 cgataagtag tcagaaagaa taattttttt agtttaaaga agaacatcag agcaaaaaaa
      661 caccaactga acaatatgtc gtttatttta aaaaaagttc ggtttttcac tttatgtaat
      721 cgctgagaaa acttttttta ataaaaaaag tcgaaaaact ctagcttttt tgtaaacggt
      781 atgatatact ataggagtag tttttgttcg gtaatctgtg gtagcttgtt tcaaataaac
      841 atcttccaga tatacaccat tcaagtaatt gcaaaatatg tgttgataca caaggaggat
      901 ttttattatg gaaacaggta cagtaaaatg gtttaactca gaaaaaggat ttggattcat
      961 cactgcagaa aacggaaacg atgtattcgt tcatttctca gctatccaag gcgacggatt
     1021 caaaacttta gaagaaggcc aagcagtaac tttcgatgtt gaagaaggcc aacgcggacc
     1081 tcaagcaaca aacgttacta aagcttaatt aacctatact aaaaaataaa cgcccagaca
     1141 tttgcgtgtc tgggcgtttt tgtctatttt ctttaaaaag cgatgagagt atttttctgc
     1201 actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga tattttgaaa
     1261 ccaagaactt atgcagtcgt tgatatcgaa acgactggga cgaatccaaa agaagatcgg
     1321 atcatccaat ttggctgcgt catgattgaa tcaggtcgga tcacttcacg tttttcgatc
     1381 gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg gatcacgaat
     1441 caacgcgtcc aaaaagcgcc ttatttcgaa gatgtcgccc aaacgattta caatcttttg
     1501 gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt gaaccatgaa
     1561 ctcatgcgat gcggacttcc tagtttgaag ataccaggca tcgatacagt agaacttgca
     1621 caaattttct tgccgacgga accaagcttt cgcttagctg atttgtcaga aagccttgga
     1681 tttattcatg agaatcctca tcaggcagat agtgatgccg aagtgacggg tcaacttctt
     1741 ttgctgatcg aagaacggat gagaaaattg ccaatcatca cgttagagca gattgcgcga
     1801 ttaagtagac acacaggtat ggatacgagt cgtttcattt atcatgtgat tgaagaaatg
     1861 aaagaaaaac cagagccact tgatccctca ttagaaatcg tcgatgggct agctttgcag
     1921 aaaaaagaag tggaactatt cacatccgtc cattatggcg agcggacata tccaagaaaa
     1981 aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga acagaacaga
     2041 ctgatgaatg cggtctacga ccatttcaca aaagatgaat caaaagattt gatgatcgaa
     2101 gcagctacag gaatgggtaa aacaatcggt tatctgctgc cattgagtta tttggctaca
     2161 cctgaaaaac ccgctgtcat cagtaccgtt tcgctagttt tgcagcagca gatcatagaa
     2221 aaagacattc cattgctgaa tcaattgcta gatcagccca tacaacctgt aattatcaaa
     2281 agctatcgcc actatattga tttacagcgt tttaaaggca cacttgtgga accgccagaa
     2341 caaaaacaat atgctttgta tcaaatggcg gtcttagttt ggctgacgca aacgaagacc
     2401 ggtgatttgg atgaattaca tttaacaaat ttgaaccatt cattctttgc agatatcgcg
     2461 cacagaggta ttggattttt agcaagaaat cagtcatttt acgaacaaga ctttgttcgt
     2521 ttcttgcaaa agaaaatcag gcaaagtaat tttttgattg tcaatcatgc gtttttaata
     2581 caagaaacac agcgaaaaga accattgctt ccagctagtc catttttgtt gatcgatgaa
     2641 gtacatcatt tgccggaaat cgctgaaaaa gtaaatgaca aacgattaag tattaactat
     2701 ttccataaac aaatccagca attagaagaa ccaaatcagc tgtttgaccg aatacaaagc
     2761 tatttaccga aagaacatga agccttacgc tggttatcga tttatcgaga agaactttct
     2821 gcgctatgcg aagcacagat ttgtatggca gaaggattaa tgagtttagt gagcggcaag
     2881 caacctaacg gacagtatcc agaagaagtc atgatgacaa aagagatgtt ggatcagctg
     2941 ccactggaat ctgggacagc cattgatcac ctgcagcttt tttatcagga gatccagcag
     3001 ctacagtcac aaatgcgtct gacttttgag attatccaag aaagctggac aaaccagatg
     3061 cggatcgatt tagcaagttt atatagctta tttgatcaat tagacaagca gtacttgctg
     3121 atggatcaat ggctggaaga ctggcgagat catttgatcc attggttcgt tcctgggaac
     3181 acagcgcagc agatgatttt tcatctccat gattttcaag cggctatgtt gccgtcgact
     3241 gtttggtatc ctcgatatga tcgtattctt tatattggag gaacgctgaa gatcggccct
     3301 aatcgacact atctctctaa gcggcttggc attgaagcag cacctcttaa agtgattgcc
     3361 tctccttatg attatgagaa acaggcaagg atctatgtgc caaaagaggc acctgccgtc
     3421 catcaagtca gcaatcaagc atatatcgat tatttagaga cagcacttac aggtctgatc
     3481 gaacaagaag atcggccaat acttgtgtta ttcacttctc atgatgtttt gcagaaggtc
     3541 tatcagaaaa tgcatctgtc tatgctagaa aaaggacgag aaatactagc tcaagggttt
     3601 ggcggaagcc gggagaagct tttgaaacgc ttcattttgt cagaaaaaag cgtattgttt
     3661 ggcgctgaca gcttttggga aggtgtagat ttgcctgggg atgctttgca gatcgttgta
     3721 gtcacacgtt tacccttcga taatccgctt cgcccgatgg tcaaagctag gaacgcttat
     3781 ttagaatcag aggggatcaa tcctttttac caggaatccg tacctaaagc agctttgaag
     3841 ttacgccaag cattaggtcg cttgataagg acggagacag atcgtggcgt attgcttatt
     3901 ttggatcgaa gactagtcac tgcaaaatat ggtgcacgga tcatccatgc attaccgaaa
     3961 aaagtaccga tcaaagagct agctatggag gaaatctatc aggacatcca tgaattctta
     4021 aagaaaaatg aagaaggaag ttagaggagc tgtctttttt cataattaaa atctgctata
     4081 atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga caaaggaaaa
     4141 aacgattaat cgcgtgttac ttggcttgat cgtattttta ttagcggtca tcgtgatcag
     4201 ctcgattttt tacttgcgtt ccagtcgtcc ggcggtgcag gcgaagaaag aagcagtaga
     4261 aatcgcaaag aaatatgcaa atcttgagga agccgatcat ttttattggt ttactcgaaa
     4321 gaagacatac ttttcactga caggtacaaa tgataaagga aaagaaatcg ccgttatcat
     4381 tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga ctgaaaacga
     4441 agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag ctgctttagg
     4501 gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg ggaaactgaa
     4561 ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg gaatttaaaa
     4621 ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga accgtctgtc
     4681 actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga tgtgctgagt
     4741 ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc agcaatcgaa
     4801 gcaatccgca acgggaaagc tagctattat acaccaacag caggaatccc agagcttcga
     4861 caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc ttcacaaaca
     4921 gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat ccttgacccg
     4981 caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca agtaaaactg
     5041 gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa agttactgtt
     5101 gaacagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa ttctccatcg
     5161 aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga atgggcagta
     5221 aaaaatgata ttttgatagt tgctgatgat atttatggtc gtttagttta taatggcaat
     5281 gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagactat catcatcaac
     5341 ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt gggaaatcct
     5401 gtgatcatca atggaatgat cgctgttgct tcccagtcaa ccagtaatcc gacagcagtc
     5461 agtcaatatg cagctgttga agcattgaaa ggcgagcaag atacagtgga agaaatgcgg
     5521 aacgcctttg aagaacgcct gaatacgtta tattcgcttg tggcagaact gccgggtgtt
     5581 tctcttgaaa agccacaagg agctttttat ctttttccta atgtaaaaga aacgctacgc
     5641 atgtgtaaat atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta
     5701 gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgctact
     5761 gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt
     5821 caaaattaat cctaaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt
     5881 caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca
     5941 aataagcgat caagcggaaa aatcgcattt ttacaattaa gagacggaac agcctttttc
     6001 caaggtgtag ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc taaaaatcta
     6061 caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt
     6121 ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc
     6181 acaccaaaag aacacggaac agacttcttg atggatcatc gtcatttatg gctgcgttct
     6241 tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc
     6301 tttaatcaac atcattttgt caaaattgat ccgccgatct tgactggttc tgcaccagaa
     6361 ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt
     6421 ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg
     6481 actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa
     6541 ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca
     6601 ttcttagtac aaaatgtttt agatcattgc gaatatgcat tagatgtact aggacgagac
     6661 aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta tgatgatgcg
     6721 atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca
     6781 cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca
     6841 aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt ggtgatctgt
     6901 gcagatatga tcgcaccaga aggttacggt gaaattattg gcggaagcga acgggcaaca
     6961 gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca
     7021 tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg tctaggtctt
     7081 gaacgaacag tcacttggat tgctggtatc gaccatgtac gtgaagctat tccattccca
     7141 cgtttgttga accgaattta tccataagag gtgatgaaaa agctgttctg catcaagtaa
     7201 ttagaacagc caagcaaaaa cagctgttca tgtttttgaa ttggcgggac ttattaccgc
     7261 agatgtaagc ttttgaatac cgtttagtgg agaggttgtg aaaaagctgt ttagtttcga
     7321 gtattaagaa aaattttgtg gaaatggctt tccacatttt tgcataaatt tggcttaata
     7381 ccgagaaact ggcttttgaa caccgtttaa ggaagaggtt gtgcgcctgc cgttcagctc
     7441 cgagtcacaa ggagtaattt caaaaaacag tttttcatgt ttgatgacta ttgtgacttg
     7501 tgaccgagga gttggccgga gagcaccgtt tattctaaaa aagaactgcg aactctcgca
     7561 gttctttttt taaaatacgg gataaaggaa gtttccagca gacaagagaa gaaagcaaaa
     7621 gaaattgcta tttgattcac aaggttctat aacgaaatcg caatcaatca aacatttaca
     7681 aagaaggtaa ttcaaatgga aatctgattt gatgatgctt ctgctgttga aataaagcag
     7741 catctagcac ctgaaaacaa actaatagta acattcgaag atggagtagg tccgtatttt
     7801 cagcatgcaa taatccatat gtaagttcaa ttcacccttg aaatcatcgg accagataaa
     7861 gttatgacca atctatccca agcaatctag aagagatatt agtaaaggga catttactag
     7921 aagtcttaga tgagcctata aatatatgtt ctgatggcgt catcagtggt ctgattgacg
     7981 gaagcttagg tgtgatcgat ctctctggac caaacaacga aggaatcaag gggaatccgg
     8041 cacgttaaga aaaaaatagc ataaaaaact agacacttga tgagcgttca ttcaattgtt
     8101 tgaagggact aggctggtgt cattttttat acatatattt tctctttcgt cttgataggg
     8161 cttatgggat aatataagga agaaaatcat cgttttattc aaaaaggagt ttttgacaaa
     8221 tgaaaaaacg tatattattt atcggtgata gcattacaga cgcaggaaga gaccgagcag
     8281 aagcaactga tctaggtaaa ggttatccac ttttgattgc cggaaaacta atctcccaat
     8341 atccaacgat ggaatttgaa tgcatgaacc gtgggatagg aggagacaaa attgcagatc
     8401 tgcaagtacg ctggcaagaa gattgtttag ctttacaacc tgactatgta tcgattttga
     8461 tcggaatcaa tgatacctgg cataatacta gcgatcaggc tatattcgga actgagaaat
     8521 ctgctcagca atttgaatcc gtttatcgaa aattactaaa tgaacttcaa acagtgggaa
     8581 tctgcaacat catcttgatg gagccgtttg tacttcctat aaccgacgac cgtcatggct
     8641 ggcgtaaaga cttagaccct aaaatccaaa ttgttcgtaa acttgctaag gaatatggcg
     8701 cattgctggt tcctttagat ggtatattca atgctttagg gattgtttat ggtttttcga
     8761 aatatacaag agaagatggg gtccacccaa cgattggcgg acatgagatc attgcacaaa
     8821 gctggataga tgcaattgcc gcagtcgatt tttttagtgg agataaaaat gagcgaaaga
     8881 aaagtgagaa atgatggaaa tacggtgggc agtaaaagaa gatctacctc aaatcaatca
     8941 gctgattact gatcatccag aatcattcgt aaaagcaaaa acggcagctg acctaaatga
     9001 taagaataaa ctattgctcg ttgctgtaac aaaagaaaaa cggattatcg gatttgcgca
     9061 agcggacgtg caacagtcag aagaacgatt ggatcagcga ccatcaggaa aactaatcca
     9121 acacacatta gtaacaaaag aaaatacgga agaaatccaa aaaaaatttg cagaatattt
     9181 gatactggaa gctaaaaaaa gaagaataaa atcatttttt gcccagtttt aatcaaaaac
     9241 agtaatagaa ggagaaatac aatgagttgt ttaggaaatg tcatctggtt tatattcggc
     9301 ggactaatcg gtggtcttag ctggttagca gcggggatac tttggtgtat cacgatcata
     9361 ggtatcccaa tcggtatgca atgttttaag atcgcctccc tttcttttgc gccttttggc
     9421 aaagaagtaa tttatcacca gtctggcatg tcattgattg ttaatatcat ctggctgatc
     9481 gtttcaggcc taccaattgc tctcgcacat gtcgtcagcg gcatccttct atgtatcacg
     9541 atcataggga taccttttgc caagcaatct ttcaaacttg ctcgattggc gttgatgcca
     9601 ttcggtgcag aaattgtata aaagagaata ataaaggtta gataaagcat agagtcaggt
     9661 gggatacatg tatttcacct gactctatgc gttctcctgt ataagttctt ccatgtgact
     9721 tttacgtctt ttatttatat ggggttactg tttatggaaa gttgaaataa ttttcattaa
     9781 agaactcttt ctatattttt gaaagcgtag tggagcttcg aatatctggg aaggacattt
     9841 tgcagggatt gaaaaaagat attgtcctat cgaaaaatta atgaatttag acaaattgcc
     9901 agcaacaggc ttcaaactta gttgctatcc tgtcaagatc aagaaggcaa gtgcgggttg
     9961 gataagagca gtggcaatga tcgaaaagaa aaagaaagaa taagccaaaa catctgtatg
    10021 gaagaaattt tccgatgcag atgtttttca ttttttcttt cggatacctt gacatatgaa
    10081 taaccgttca tttataatta gttatgaaga gatattcata tgttgaggcg aggaggtcat
    10141 taaaatgaaa agttacagat tagaaggttt agattgtgcg aactgtgcaa tgaaaatcga
    10201 aaaaggagtt cagaagatca atggagtcaa ggaagcaacg gtaaacttca cttctggaaa
    10261 actaacgatt gacgcagagg aagatcattt ggctacgatt gaacaagaaa taaaaaaagt
    10321 agtgaaggaa ttagaaccag atgtcaaagt aacagaaata gataaggaaa aagtatctga
    10381 acatggaaat gaaaaagaga gaaatacgct gttccggatt ttattttccc ttgcaggtat
    10441 cgctttattg ctattatttg attttaatga accaattcgc ttgatagggt atttattgat
    10501 ttatctgctg ataggatatg atgtagtgaa gaaggcagtg atgaatatcg tcaaaggaaa
    10561 aatctttgat gagaattttc ttatgtctgt tgctacgatt ggcgctatgt tgatcggtga
    10621 atatccagaa gccgtagcag tgatgctatt ttatcaaatc ggcgagtatt tccaaggatt
    10681 ggcagtcagt cattcaagaa aatcgattcg cgaattgatg gcgatccgtc cagaaacagc
    10741 tcacgtgcag acgacggaag gattgatgac cgtgaaccct gaagacgtgc agatcggtca
    10801 gtttgtactt gtgaagcctg gtgaacgtgt accgttagat ggaacgatca tcgaaggaga
    10861 atcattagtc gatacgtcag cacttacagg agaatcagtc cctaaatctg tatatgttgg
    10921 tgaaacagta ttaagtggat ttatcaataa aaataaacca tttttagttc aagtagaaaa
    10981 atcttatgaa aattctacta tcagcaaatt attagaatta gtagaaaatg ccagcagtaa
    11041 aaaagcacca gctgaaaatt tcattactaa attcgcgcgc tattacacac cagtcgttgt
    11101 aggattggcc gttttattag ctgttttacc tccacttgtt gtttctggag cggcgttttc
    11161 tgaatggatc tatcgagcat tgacattttt agtgatctca tgtccatgtg cattagtgat
    11221 ctccgtaccc cttagtttct ttggcggtat cggaggagca agtaaaatcg gtgtattagt
    11281 aaaaggttca aattacttgg agttgcttgc acaaactgag acagtggtat tcgataaaac
    11341 cggcacatta acgaaaggtg acttttcgat ccagacgatc gatacaaaaa tcgatccaaa
    11401 gatattcatg caatatgttg cgagtgcaga acagttttct acacatccga ttgctcaatc
    11461 tgttttagaa ggctacgcag gtccgctttt accaacagct aatatccaag aatttgcagg
    11521 tgaagggatc ttagctgaag ttgatggcaa acaagtatta gcaggtaatc acaaattgat
    11581 ggaaagattt gaaatctctt ttccttcaag tcaagaaatc ggtacattgc tttatctagc
    11641 gatcgatcaa agttacagtg gttatcttgt tattgcagat accttgaaag aagatgccgt
    11701 agacgcattg gttcaattaa aaaaggctgg tgtgaagaac acagtcatgt tgacgggcga
    11761 ttcgaaaaaa attgctgatc atatcgggaa gcaagtaggc gtggacaaga tatatagcga
    11821 attgcttcca gaagataaag tgcagcggtt agaggaaatc ttacaaagaa acaataaaaa
    11881 gaccgcattt gtcggagatg gaatcaacga tgcaccggtg ctagcaagag cagatgtagg
    11941 aatcgcaatg ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat
    12001 gaatgatcaa ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa cattgaaaat
    12061 cgtcaaacag aacattgtct ttgctattgg gataaaaatt ttggtacttg ccttaggtgc
    12121 ttttggtttt gcatcaatgg gagatgcagt atttgcagat gttggggtga cagtattagc
    12181 tgttttgaat gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac
    12241 gaatgaacgt cctctttttt ttatatcctt ttcttaatga aaaatgtaaa gaaaatagat
    12301 aggattataa aataactaat gtttttaatt ttgcagaaaa ttcaaaatag cttgaaattc
    12361 aataaggtaa atgaaataaa agaaaataac ttattttttt gatggaaaaa atatgaaaaa
    12421 acgatgtgta ttaaataaaa gaatacacac ataaaataac actgtcttgt aagtttgtta
    12481 aaaattgaat atgattacat ctggataaca aatttttatc ataaaggaga gaacgaatga
    12541 acaacaaaca attacgctgg tttactgttg cgttaattgc atttaacatg gtttggggca
    12601 tggggaatgt agttaataac tatgctcagc aaggagtatc agttgtcacc tcttggctat
    12661 tgattttggc catttatttt attccatacg cattgatcgt tggacaatta gggtcagcct
    12721 tcaaggatag taaaggaggc gtcagttcat gggtcgaaaa tacgacatct aataaacgac
    12781 tggcatacta tgccgcttgg acttactggg tcgtccatat tccttatttg gcacaaaagc
    12841 ctcaagcaat cctgattgct ggcggttggg cagtccaagg aaatggaaat atcgtaaata
    12901 cgatgtcagt acaaatggta gcagttattt cacttattat atttcttgcc tttttatatt
    12961 tatcaacaaa aggactttct actttgaaag tgattggcgg actggccgga acagcgatgt
    13021 tcgtcatgtc gatcttattt attcttttag ctgtaacggc accgttcatc aatccaacga
    13081 tggaatttgc cacaccacat atgaatcaag tgaaaacata tattccaaca tttgatttca
    13141 gttatttcac aaccgtttcg atgctagtat ttgcagttgg aggagcggaa aaaatatccc
    13201 cttatgtcaa ctctacaaga aatcctgcaa aggaatttcc taaagggatg atcttcttag
    13261 cagcaatggt agggatttgt gctgtattag gctcagtcgc aatgggtatg ctgtttgcta
    13321 gcgataatat tcctaaagac ttgatggcaa atggggcata cagtgctttt caaattttag
    13381 gtaattacta tggtatcggt aactcattga tgatcattta cgcattgaca aacatgattg
    13441 gacaaatctc cgcattagcc ttttcaattg atgcaccact aaaaatcttg ttagcagatg
    13501 cggatccaga atttgtacca aactggctac gtaaacgtaa tcgtaaaggt acattggtga
    13561 atggttatct tctgacaggt atactagtag gaatcattat tttgttgccg atctttggta
    13621 tcaaagatat gaatgaatta gtaaaatggt tgacgaactt gaactcagtt gtcatgccaa
    13681 tgcgttacct atgggtgttc ttggcatata tgatgttgaa taaagcttat aagaaattca
    13741 attctgaata taaatttgtt aaaaatccaa aaatcggttt tatctttggt gcttggtgct
    13801 ttgccttcac tgcgtttgca tgtatcctag gaatggtgcc aaaagtagat tatactgctg
    13861 atcctaaagc atggtggttc caattattca cgaatatcat cacaccaatt gtcttgatct
    13921 tattaggtat gatcttgcca gcaattgctc gtcgagacaa aaacaaagaa atcactgaac
    13981 cagaaacagt ggttcagcct taaaatagaa atacttgtta caagaggttg tgacagaagt
    14041 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaagt
    14101 ttcggcttgt ttttcgcaga agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa
    14161 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcccctcat atttttcgtt
    14221 ttgctgggct tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggtagtga
    14281 gcctgccgtt taactccgag tcacaaggag caatttcaaa aaacaacttc tcatgtttgt
    14341 tgactattgt gccttgtgac cgaggtgttg gcaggagaac accgtttaag gaagaggttg
    14401 taaaatcagc gatttgacct gagtattaga ggaacaaacg aaaaaatagc tcctcatatt
    14461 ttgccgtttt ttgagttaat gctgaaggtc agctgattga acaccattta ttcgaaacaa
    14521 gggactttga cacgactttt gtcacagtcc cttgtttttt tgcggcgtta taagtggtat
    14581 gatcgaacca aattccaaag agaagtgagg gagaagaatg aagatcatcg tgattggaca
    14641 aagtgggtcg ggaaaatcga ccctcgcaag gaagatcaaa gaaatcaccg actttccgct
    14701 actgccgcta gaccttttat ggcatacaac agactacagt attcaggcaa aaaagtggtt
    14761 tttgcaagaa cagcaatcgt ttatggtatc taatccgtca tggattgttg aaggaaatta
    14821 tacttctaca ttagaggaaa ggattaaaga agcagataag atcatttggc ttaaagtgcc
    14881 gcgttatctc gccatctacc gagtagtttg gcgttctcta aaaagaaaga taaacaaaaa
    14941 atctcggcca gatatgcctg aatcattttc agagaaacta aacagggaat acttagaatt
    15001 tttatctttt atctggcatt ttgaagaaaa caatgaaccg aaaatacagc agctgatcca
    15061 agtagctaat gcaagaaata aactgatcat ccttaaaaca agacgagaga aacaagaatt
    15121 tttaactaaa ttgaaaatag agagcaaaaa taattgacag tttttgttta caagcgtaat
    15181 ctgtattcat aagctaatga ttacatttgt aaacgaaagg tgtgaaataa atggaaaaag
    15241 tcaaaacgcc agtaaagatc agtgattcag agtgggagat catgcgtgtt atttggacac
    15301 ttggacagac gacagcccaa gaaatcacgc agatcttagc tgattcgatg gattggaaac
    15361 cagcaacgat caaaactttg ctgggacgtt tggtaaaaaa agaagtgatc tggacagaac
    15421 aagaaggaaa aaaattcatt tatcatccag ctgtctcgga gatggaaaca gttcgcagtg
    15481 cgacggaaaa tcttttttcc catatctgtg caaaaaggat tggagagacg attgcggatc
    15541 tagtagaaga agccactttg actcaagaag atatcaatga gattttgaaa caactggaac
    15601 aaaagcaacc agtagatacg atcgaatgta actgtatccc aggtcaatgt gaatgtaaac
    15661 aacatcaata ggaggaagaa aaaatgaaac aacaattttc aatcgaaggt atgagctgca
    15721 atcattgtgc agcaagagta gaagaaacag taagcgcact ttacggcgtg caaaaagtga
    15781 aagtaaattt gaaaaaagca aacggtacgg tcaagtttga tgaaacaaaa gttcaatccg
    15841 aaaagatttg tcaagcaatc aatggattgg gttataaagc agaggtgatt tgatggcaac
    15901 agcagaaacc aaaatggata cgttcgtcat tacaggtatg acttgtgcaa attgttctgc
    15961 taggatcgaa aaagagctga acggacaacc tggtgtggtc aatgccacag tcaacttggc
    16021 cactgaaaaa gcaagtgtca aatatgaagg gacgaccact gaaaaattaa tccaaagcgt
    16081 agaaaacatc ggttatggtg caattttgta cgatgaagcc cacaaacaaa aaattgcaga
    16141 agaaaagcaa gcgtatttga aaaaaatgct attcgatctt atattgagta cggttttgac
    16201 tttgccgttg atgttgtcaa tgattgctat gatgttagga agccatgcgg cgattgttca
    16261 cttttttcat tttccaatcg tccagttagt gctgtctgct cctgtccaat tttatgtcgg
    16321 tgctcgtttt tataaaggtg cgtaccatgc aatcaagacg aaagcaccaa atatggatgt
    16381 attggtagca attggaacat cggctgcctt tgcattgagc atttacaacg gatttttccg
    16441 tggacacccc caagatcttt attttgaaag cagcagtatg atcatcacgc tgattttgct
    16501 cggcaaatat ttggaacata cagcaaaaac gaaaacaggt aatgcaatca aacaattgat
    16561 gtctcttcaa acaaaaaccg cccaagtgat aagaaacggg aaagaagaaa cgttagcgat
    16621 tgaagaagta gtcgttggtg atcaactagt catccgtcct ggtgagcaga ttccagcaga
    16681 tggtcgaatt atttcaggaa gcagtgcgat agatgaaagt atgcttactg gtgaaaatct
    16741 tcctgtagaa aagaatccgg atgacacgct ttttggcggg acaatcaata cgaatggtct
    16801 gctacacatg gaagtcacac aagtcggcaa gcagacagta ttagcgcaaa tcattcaaat
    16861 ggtggaagat gctcaaggga gcaaagcccc aatccaaaaa atcgcagatc ggatctcagg
    16921 gatttttgtt ccgatcgtat tagtcatcgc gtttatcacg ctcatagcta caggtttgat
    16981 cactggtgat tggcaactcg cattgatcca tagtgtctct gtattagtca ttgcgtgtcc
    17041 atgtgcatta ggtttagcga ccccaacagc tattatggta ggaacgggtg ttggtgcacg
    17101 aaatggaatt ttaattaaag gcggagaagc attagaagca gctgctcact tagacagcat
    17161 tgttttggat aaaactggaa cgattacaga aggaaagccc aaagttacag atctagtcgg
    17221 ttcaaaagaa gtcctatcta ttttttatac tttagaacaa gcttctgaac atccactagg
    17281 taaagcaatc gtggaatacg gaaaactgca agaagcagca acatacgata tgattgattt
    17341 tacagcccac ccaggtgctg ggatcagcgg tacgattaat ggagtacgct attttgcggg
    17401 tacacgtaaa cgattaattg aattgaacct atcatttgat gaataccaag aacacgcgtt
    17461 agaattagaa cagcagggaa aaacagtcat gtttttagct gatgaaaagc aagtgatcgg
    17521 gtggattgcc gtggctgatc aaatcaaact agaagccaaa caagcaatca agcagctcca
    17581 gaataaagga ttagatgttt ttatgctgac aggagacaac aaactggcag ccgaaacgat
    17641 tggtaaacaa gttggtattg atccaaagca tatctttgct gaagtacttc cagaggataa
    17701 agcagcctac gtagaaaaac ttcagaaaga tggcaaaaaa gtcggtatgg caggcgatgg
    17761 aatcaatgat gcaccggcct tagcattagc tgatgtcggt atggcaatgg gaagtggaac
    17821 ggacattgcg atggaaacag ccgatgtgac gctgatgaat agcagtttgg catccattgc
    17881 acaaacaatc gagttgtcta ggttgacatt gagaaaaatc aaacaaaatc ttttctgggc
    17941 ttttgtctat aatacgattg gtattccttt tgccgcacta ggctttttga atccaatcat
    18001 tgcaggagga gcgatggcgt ttagttccgt cagtgtattg ttgaactccc tgagcttaaa
    18061 tcgacacatg aaaaagtaag agaagggatg gagtaaaaat gaatgggaac gatatggaga
    18121 aaaaacatga gcataaaaaa gaagtagtaa acaaagaaag tacagaatct catgaaaaac
    18181 agatggagat gaaacatgac catagtcaga tggatcattc catgcacatg ggacataacc
    18241 atggagatat agcaaacagc aaacaaatgg accattcgat gcacatggat cacgaacacg
    18301 gcggcatgga tcattccatg cacatgggga atttcaaaca aaaattttgg ctatcgctta
    18361 tcctagctat cccaatcatt cttttttcac caatgatggg aatggaattt ccatttcaag
    18421 taacgtttcc tggttctgat tggctcgtat tgatcttagc aactatacta tttatctatg
    18481 gcggtcagcc atttttaagt ggagctaaga tggaattgaa acaaaaaagt cctgctatga
    18541 tgacactgat cgcgatgggg attacagttg catatattta tagtgtctat tcgtttatcg
    18601 caaatctgct caatccacat acacacgtca tggatttctt ctgggaattg gctactttga
    18661 tcgttattat gctactaggt cactggattg aaatgaatgc tgtgtcgaat gccagcaatg
    18721 ctttacaaaa actagcagaa ttactgccag aatcagtgaa acgcttgacg aaagatggaa
    18781 aagaagagac agtttcattg aaagaagtaa acgaaggtga tcgtttgatc gtccggtctg
    18841 gtgataaaat gccaacagat ggtgtaatct tgaaaggtga aacaattgtt gacgaatcag
    18901 ctgtgactgg cgaatcaaga ggaatcaaaa aacaagccaa tgacaaagtg attggcggtt
    18961 ctatcaatgg ggatggaacg atcgaaatcg aagtaactgg aactggtgaa aatggttatc
    19021 tagcgaaggt tatggaaatg gtgcgaaaag cacaaggaga aaaatccaag ctagagtcat
    19081 tatctgacaa agtggcaaaa tggctgtttt atgtcgcatt aattgtgggt atcctggcat
    19141 tcatcgcctg gttgttcttg acagatcttc ctaatgcatt agaacgtatg gtaacagtct
    19201 ttatcatcgc ctgtcctcat gctctaggtt tagctatccc attagttgtt gctcgctcca
    19261 catcaatagc agctaaaaac ggattacttt tgaaaaaccg caatgcactg gagcaagcca
    19321 atgatctaga tgtgatcatg ctggacaaaa caggaacact aacagaagga aaatttactg
    19381 taacgggtgt cgaagtactt gatgatgcgt tcaataaaaa tgaaatcctt caatatctag
    19441 gagccttgga agcgaatgcc aatcatccat tggctgttgg cattatgaat tatttgaaag
    19501 aacatgaaat caagccttat caagctgaaa atctaaaaaa tctttcagga gtcgggctag
    19561 aggcaacagt caaaaaacaa caagtgaaaa tagttaatga aaaagaagtt gagcgtctcg
    19621 gattaaacgt agaacaagca ttattgaaac cttaccaaga acaaggaaat accatcagct
    19681 tcctgatttt agaaaatcat ctagctgcaa tcgttgcttt aggtgacgta gtaaaaacag
    19741 aagcaaaaga atttatccga acattgaaag aaagaaaaat cactcctgtc atgcttactg
    19801 gtgacaataa aaacgctgcc caagcagtag cagattattt agggatcgaa gaatattatg
    19861 gcggtttatt accagatgat aaagaagctg ttgtccagaa ataccttgac caagggaaaa
    19921 aagtaatcat ggtgggtgat gggatcaatg atgcgccaag tttagcccgt gcttcaatcg
    19981 ggatggcaat cggtgcaggg acagatatcg caatcgattc tgcggatgtc gtattgacaa
    20041 acagcgatcc aaaagatatt ttgcattttc ttgatctagc caagcaaacg cgaaagaaaa
    20101 tgatccagaa cctctggtgg ggggcaggtt acaatattct tgcaattccg ctagcagccg
    20161 gaattcttgc gccaattggt atcatattaa gtccagcagt tggtgctgtc ttgatgtcgt
    20221 taagtacggt agttgtagct ctaaatgctt tgacattgaa aatcaaataa taatgaaaaa
    20281 a
//
LOCUS       DACCCM010000055        20180 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCCM010000055 DACCCM010000000
VERSION     DACCCM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.058"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IVK78_001918"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IVK78_001918"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP6375373.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IVK78_001919"
     CDS             1011..2258
                     /locus_tag="IVK78_001919"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP6375374.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2677
                     /locus_tag="IVK78_001920"
                     /pseudo
     CDS             2334..2677
                     /locus_tag="IVK78_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2794..3120)
                     /locus_tag="IVK78_001921"
     CDS             complement(2794..3120)
                     /locus_tag="IVK78_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375375.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3493..4695)
                     /locus_tag="IVK78_001922"
     CDS             complement(3493..4695)
                     /locus_tag="IVK78_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375376.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4812..5327
                     /locus_tag="IVK78_001923"
     CDS             4812..5327
                     /locus_tag="IVK78_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6375377.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="IVK78_001924"
     CDS             complement(5526..5972)
                     /locus_tag="IVK78_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6375378.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IVK78_001925"
     CDS             complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IVK78_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6375379.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="IVK78_001926"
     CDS             complement(7851..8480)
                     /locus_tag="IVK78_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6375380.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="IVK78_001927"
     CDS             complement(8464..8928)
                     /locus_tag="IVK78_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6375381.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="IVK78_001928"
     CDS             complement(9415..10683)
                     /locus_tag="IVK78_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6375382.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="IVK78_001929"
     CDS             complement(10914..11651)
                     /locus_tag="IVK78_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6375383.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="IVK78_001930"
     CDS             complement(11718..12287)
                     /locus_tag="IVK78_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6375384.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="IVK78_001931"
     CDS             12415..13287
                     /locus_tag="IVK78_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6375385.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13629..14501
                     /locus_tag="IVK78_001932"
     CDS             13629..14501
                     /locus_tag="IVK78_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6375386.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14498..16456
                     /locus_tag="IVK78_001933"
     CDS             14498..16456
                     /locus_tag="IVK78_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6375387.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16707..17558)
                     /locus_tag="IVK78_001934"
     CDS             complement(16707..17558)
                     /locus_tag="IVK78_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6375388.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17681..18148
                     /locus_tag="IVK78_001935"
     CDS             17681..18148
                     /locus_tag="IVK78_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6375389.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18411..18893)
                     /locus_tag="IVK78_001936"
     CDS             complement(18411..18893)
                     /locus_tag="IVK78_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6375390.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18993..19856)
                     /locus_tag="IVK78_001937"
     CDS             complement(18993..19856)
                     /locus_tag="IVK78_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6375391.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6066 a         4155 c         3663 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatgtt gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc
     2581 aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca
     2641 gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga
     2701 gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt
     2761 cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc
     2821 ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc
     2881 atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg
     2941 ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc
     3001 ttccgcattg attattggca attctttgat atagccacga taaatcaacc gatcatctga
     3061 aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat
     3121 ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca
     3181 gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc
     3241 cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat
     3301 gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc
     3361 tcttggtaaa cggcgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3421 tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag ctttttcaca
     3481 acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat aaatgataca
     3541 acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt caccaatatg
     3601 ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc ggacaaaact
     3661 atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa tcgtcgtcaa
     3721 cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg gtacaaggac
     3781 tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga caaatgctgc
     3841 taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga aaatcgaagc
     3901 aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt cagaatagcc
     3961 aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat ataacaatgc
     4021 gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt cccaaaggcc
     4081 tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta taaaaccgca
     4141 aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc ccaagacccc
     4201 accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca tcatgattgc
     4261 tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag agacaaataa
     4321 agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat tcccagataa
     4381 tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg tttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgatga atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    13441 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    13501 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    13561 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    13621 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    13681 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    13741 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    13801 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    13861 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    13921 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    13981 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    14041 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    14101 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    14161 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    14221 tttcactgaa ttattcaaga ctggtgaatc atttacgact acttctacaa cgaacccatg
    14281 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    14341 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    14401 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    14461 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    14521 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    14581 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    14641 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    14701 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    14761 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    14821 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    14881 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    14941 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    15001 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    15061 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    15121 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    15181 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    15241 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    15301 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    15361 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    15421 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    15481 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    15541 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    15601 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    15661 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    15721 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    15781 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    15841 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    15901 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    15961 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    16021 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    16081 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    16141 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    16201 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    16261 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    16321 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    16381 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    16441 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    16501 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    16561 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    16621 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    16681 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    16741 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    16801 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    16861 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    16921 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    16981 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    17041 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    17101 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    17161 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    17221 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    17281 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    17341 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    17401 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    17461 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    17521 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    17581 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    17641 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    17701 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    17761 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    17821 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    17881 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    17941 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    18001 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    18061 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    18121 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    18181 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    18241 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    18301 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    18361 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    18421 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    18481 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    18541 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    18601 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    18661 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    18721 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    18781 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    18841 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    18901 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    18961 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    19021 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    19081 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    19141 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    19201 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    19261 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    19321 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    19381 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    19441 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    19501 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
    19561 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    19621 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    19681 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    19741 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    19801 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    19861 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    19921 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    19981 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    20041 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    20101 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    20161 cgctgcttgg atttttcttt
//
LOCUS       DACCCM010000056        19450 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCCM010000056 DACCCM010000000
VERSION     DACCCM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.059"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            126..3653
                     /locus_tag="IVK78_001938"
     CDS             126..3653
                     /locus_tag="IVK78_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6375392.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IVK78_001939"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IVK78_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP6375393.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IVK78_001940"
     CDS             7441..7782
                     /locus_tag="IVK78_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6375394.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IVK78_001941"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IVK78_001941"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP6375395.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IVK78_001942"
     CDS             9139..11559
                     /locus_tag="IVK78_001942"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP6375396.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVK78_001943"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVK78_001943"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP6375397.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="IVK78_001944"
     CDS             12993..13730
                     /locus_tag="IVK78_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6375398.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="IVK78_001945"
     CDS             13744..14559
                     /locus_tag="IVK78_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6375399.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IVK78_001946"
     CDS             14572..15219
                     /locus_tag="IVK78_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6375400.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IVK78_001947"
     CDS             15235..15891
                     /locus_tag="IVK78_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6375401.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="IVK78_001948"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="IVK78_001948"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP6375402.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="IVK78_001949"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="IVK78_001949"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP6375403.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="IVK78_001950"
     CDS             18233..18751
                     /locus_tag="IVK78_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6375404.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="IVK78_001951"
     CDS             18858..19358
                     /locus_tag="IVK78_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6375405.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3340 c         4177 g         5392 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc
//
LOCUS       DACCCM010000057        18894 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCCM010000057 DACCCM010000000
VERSION     DACCCM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.060"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(20..250)
                     /locus_tag="IVK78_001952"
     CDS             complement(20..250)
                     /locus_tag="IVK78_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375406.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(686..1252)
                     /locus_tag="IVK78_001953"
     CDS             complement(686..1252)
                     /locus_tag="IVK78_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6375407.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1252..2208)
                     /locus_tag="IVK78_001954"
     CDS             complement(1252..2208)
                     /locus_tag="IVK78_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP6375408.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2208..2864)
                     /locus_tag="IVK78_001955"
     CDS             complement(2208..2864)
                     /locus_tag="IVK78_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6375409.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3234..5252
                     /locus_tag="IVK78_001956"
     CDS             3234..5252
                     /locus_tag="IVK78_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6375410.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5679..8093
                     /locus_tag="IVK78_001957"
     CDS             5679..8093
                     /locus_tag="IVK78_001957"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP6375411.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8441..9994
                     /locus_tag="IVK78_001958"
     CDS             8441..9994
                     /locus_tag="IVK78_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6375412.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10375..12159)
                     /locus_tag="IVK78_001959"
     CDS             complement(10375..12159)
                     /locus_tag="IVK78_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375413.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12174..13895)
                     /locus_tag="IVK78_001960"
     CDS             complement(12174..13895)
                     /locus_tag="IVK78_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375414.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13995..14756
                     /locus_tag="IVK78_001961"
     CDS             13995..14756
                     /locus_tag="IVK78_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6375415.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15723..15991
                     /locus_tag="IVK78_001962"
                     /pseudo
     CDS             15723..15991
                     /locus_tag="IVK78_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16663..>18894
                     /locus_tag="IVK78_001963"
     CDS             16663..>18894
                     /locus_tag="IVK78_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375416.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3352 c         3786 g         5551 t
ORIGIN      
        1 tcattaaaaa gcataaccat tactccgatc tgtttttaaa attagtttct acctctaata
       61 tatatcgagt ttttattttg ccttcagaga atactgtttc tttttttgca gttacaggtt
      121 gtttattttc ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt
      181 ttttactgct ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt
      241 cttccatcat aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg
      301 atatttgagg ggcaaaaaag gggcataatt tgactgatat taagttagct attgatatat
      361 gaatgacata caaaaatata aaatctttta aaaatgctta tatatcaatg tttttgccat
      421 tcttttatat atctgttata tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata
      481 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct
      541 tctcatgttt ttgatttggt tggacttatt accgcagata taagcttttg agcaccgtga
      601 ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat
      661 atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat aaaataggcg
      721 gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc tgtggcagtt
      781 tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga ccatagtaga
      841 cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc
      901 gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac cctagattga
      961 aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt
     1021 gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca cgagtagcat
     1081 cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg atgtgttctg
     1141 ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga tcccctggtt
     1201 gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac atcagacgct
     1261 cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa gttcttcatc
     1321 aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca aggaatccca
     1381 cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt ccagctggtc
     1441 gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt catgatagtc
     1501 ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc cttggatatc
     1561 tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt cgcctggtaa
     1621 tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag ccacgccatc
     1681 taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt caaaagttgt
     1741 ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa ggtaatcaac
     1801 tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga cacctggtaa
     1861 attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg tgtttgtaaa
     1921 attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca tcatgtcgat
     1981 ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc
     2041 agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat tcggacagtc
     2101 aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc gtaaagcgta
     2161 attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta aaagtccttc
     2221 tttctgtaag agttatctat ttgttttctt ttttctctat acactggata agtcaggcac
     2281 agccaagaaa gactgagtaa gtagccgccg atgatatcag taggaaaatg aacgcccaga
     2341 taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg aagcaatgtg
     2401 cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc gtaaagaatc
     2461 atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac taagtgcgcc
     2521 aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt
     2581 acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata ccacatccat
     2641 aaagccgcac aagcaaataa aatgatgacg gtaagaggat taccgaattt agtcataaag
     2701 agttggtagc tgttcagatc aggataagaa gctctgacaa tgcctgtaaa ccactgatcg
     2761 aaaccagaaa gccacgacag atggaacttt acgatccagc ccaaaatgaa aaacaggaca
     2821 agaaagaagc taccagccac ctgaaatgat gttttatatt tcatacaatt cctcctttga
     2881 agcaatgact tattatagca tttttggtcc agcgaaaaaa acggtaaaga gataaaacca
     2941 atgtatgatt cctctttgaa taaccaatat tcttataaaa aaaataactg taatcggaaa
     3001 gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc
     3061 cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac
     3121 ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat
     3181 taagagaagt tataattatt aataatagat aaattgaagt tggaggatag aagatggaaa
     3241 acattgcaag aaaagaacgg agacgtttag aacagaccaa acgttttcga aaagcaaaac
     3301 gaggggcagc aatcgtggga actgcgatgg taggctgttc tgtcgctgcc ccattagtac
     3361 agccggttcc tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga atcaatacag
     3421 cggcatttat cacagaaatt gctacttatg ctcaaccaat cgctcaagcc aacgatctat
     3481 atgcatcagt gatgatcgca caagcagttg tagaaagcgg ttggggcagt agtgcgttat
     3541 ctcaagcacc ttattataat ctttttggga taaaaggcag ctatcaagga caaaccgtct
     3601 acatggatac gcttgaattt ttaaataatc aatgggtaaa taaaaaggag cctttccgac
     3661 aatatccttc ttttgctgaa tctttcaatg acaatgccta tgtacttaga aatacttcat
     3721 ttggcaacgg ttattactat gccggtactt ggaagagcaa cacgaaatcc tatacagatg
     3781 cgacagcttg tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc aaattaaata
     3841 atatcatcgc aacttacgga ttgacccaat atgatacacc agctagcgga aacgcaggcg
     3901 gtggagcgac tacaggtggc aataatactg gaagtgcagg tagtactggc ggttcaagca
     3961 attctggtaa tacaaactca ggttcagcaa cttcgaccac ttatactgtc caatctggtg
     4021 attctgtttg gggaatctca aataaattcg gtatcacaat ggatcaattg atccaatgga
     4081 acaacatcca aaacaatttc atttatccag gacaaaaact gacaatcaaa ggtagccagg
     4141 aaaatggctc ttcgacgaat aattccggta acaataccaa ctcttcagga aatgctggta
     4201 catcaaacgg aggacaaaca actggtgcga aatacactgt tcaatctggt gattctgttt
     4261 ggaagatctc aaatgatcac ggaattacga tggctcaatt gattgaatgg aacaatatca
     4321 agaacaattt cgtttatcca ggacaacagc taatcgtatc aaatggcggt tcttctaatg
     4381 ctggagcaaa tactggctca aatactggga atacaagtgg ctctagttcg tccagctcaa
     4441 gttcttctaa tacagcttca ggaacgaaat atacagttaa agccggagac tctgtttgga
     4501 gcgtctcaaa taaatacgga attacgatgg ctcagctaat ccaatggaac aatatccaaa
     4561 ataactttat ctacccagga caacaactga ctgtttcaaa cagtggatca acaaacagca
     4621 cgtcaggtaa tacaggttcg tcaaccaata caaataacag cagttcagtt aatactggca
     4681 gcagttcgtc tcaaggcact tataccgtga aagcaggtga atctgtttgg agcgtggcaa
     4741 ataaaaacgg catcacgatg gatcagttaa tcgaatggaa taatatcaaa aacaacttca
     4801 tttatccagg acagcaattg atcgttaaag gtggaacttc ggcaaataca aatacaggtt
     4861 caactacttc tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca agtacatcat
     4921 ccactggtaa tacgatgtat accgtgaaag cgggcgagtc agtttggagc gtagcgaaca
     4981 aacatcatat cacgatggat cagttaatcg aatggaataa tatcaaaaat aatttcatct
     5041 atccaggaca gcaagtgatt gtgaagaaag gttcatctca atcaacaaac caacaatcag
     5101 ttactggttc gaaaacctat actgtgaaat ctggagaatc tgtttggagt gttgctgaca
     5161 gtcatggaat tacaatggct caactaatcg aatggaacaa tattaagaac aactttattt
     5221 atccaggtca aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa taacgattga
     5281 attccacaaa aatatttgtt actataaggc tacttaaata tgacaacact tgaaaaggaa
     5341 gtagtaaaaa gactgaagtt tcagagagcg catggctggt gagaatgtgc acggatgctt
     5401 ttgaactcgc ctttgagttg cccttgctga atacccgagt aagcaagcgg cggtgacggc
     5461 cgttacaacg ttcgaggcct tttcactaag gtaaaattag gtggtaccgc gtagattacg
     5521 tcctataaaa agagcaatct ttttatagga tttttttatt tcttttgtga agtttgagaa
     5581 aacaaagcaa aaggaaataa aaataaacag attctgccgg ttttttcgtt atatttataa
     5641 tagaaacaag tgttttcaat agaacaggag gacagcaaat gagttacaat cataaagaaa
     5701 ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac tttcaacacc catgatgatc
     5761 ctgaaaagcc aaaattttac gcactggata tgtttcccta tccatccggt caagggctac
     5821 atgtagggca tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc
     5881 aaggatacaa cgtgctgcat ccaatggggt gggatgcatt cggtctgcct gctgaacaat
     5941 atgcgctaga tacaggaaac gatccagctg agtttaccca aaaaaatatc gaaacattcc
     6001 gacgccaaat caattcttta ggatttagtt atgactggaa tcgcgaagtg aatacaactg
     6061 atccggagta ttataaatgg acacaatgga tctttacgaa gctttatgaa aaaggtctag
     6121 cttatgaagc agaagtagcg gtaaactggg tacctgaatt aggaacagtt atttcaaatg
     6181 aagaagtgat cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc
     6241 gccaatggat gctgaaaatc actgcttatg ctgatcgctt attggatgat ctagaattag
     6301 ttgattggcc agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag
     6361 caaatattga atttaaagtg gctggtacgg ataaatccta taccgtattt acgacacgac
     6421 cagatactct attcggtgca acttactcgg ttcttgcacc tgaactagat cttgttagag
     6481 aaattacgac ccctgaacaa aaagctgctg tagaagctta tatcgaagag acagctaaaa
     6541 aatcagatct aaagagaacg gatcttgcaa aagaaaaaac aggagtattt actggtgctt
     6601 atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg gatcgccgat tatgtattat
     6661 cttcatacgg cacaggagcc atcatggctg ttccagcaca tgatgaacgt gactttgaat
     6721 tcgctcaaac tttcggacta gaaattcttc cagttatcga aggtggagat gttgaaaaag
     6781 ctgcttacac agaagatggt acgcacatca actctgaatt tctagacggc atgaacaaac
     6841 aagaagcaat cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag
     6901 tcagctatcg cttacgtgac tggctattct cccgtcaacg ttactggggt gaaccaatcc
     6961 caatcatcca ttgggaggac ggaacagtca cagctgtccc agaagaagag ctaccattac
     7021 ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg agaatctcca ttagcaaata
     7081 tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga
     7141 atacaatgcc acaatgggca ggaagctcat ggtattacct gcgttatatc gaccctcata
     7201 acaaaaaaga actagcagat tatgaaaagt tgaagcgttg gctaccagtt gacatctaca
     7261 ttggtggagc agaacatgcg gtacttcatt tactatatgc acgattctgg cataaattct
     7321 tatacgatat cggtgttgtt cctacaaaag aaccattcca aaaactatac aatcaaggaa
     7381 tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg
     7441 atgatgtggt cgaaacttac ggtgcagata cactgcgcat gtacgaaatg ttcatggggc
     7501 cattagatgc ttcgattgct tggagcgaaa atggcttaga aggaagccgt aaattcttgg
     7561 atcgtgtatg gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt atcactacat
     7621 tgaatgatgg caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg acagaagact
     7681 atgaaaatct tcatttcaat acagcaatct cccaattgat ggtctttatc aatgaagcct
     7741 ataaagtgga cgctctgcct tatgaatata tcgaaggatt tgttcaacta ctcgcaccga
     7801 ttgctcctca catgggtgaa gaactatggg caatccttgg aaatgatggt ggtatttctt
     7861 acgcaccatg gccaacatac gatgaagcag cacttgttga agacgaagta gaagttgttt
     7921 tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg
     7981 agctagaaaa agcagcatta gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag
     8041 tcagaaaagt tattgcggta ccaaacaagt tagtcaatat cgttgctaat taattggctc
     8101 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggccagaat gaacttcatt
     8161 ctgtctcaga ttttttgtta tttcaaattg attaattgat tgatcaaaaa ggttttgatt
     8221 ttcatgttct caaacacgtt caatgtcttt ttcttctgaa tggtcttgta agagatctct
     8281 gtaatcatca aatttttgtc tattaatctt agtatttttt gatgtataaa ctacctgaaa
     8341 ttgagagtac ataccaaatt cataaatttt ttattagtgg gactagtgat tgtatggatt
     8401 ttgacgcata atgaatgaag tatgagaaaa ggagagacaa atggatcaag aactatttat
     8461 tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag
     8521 aataatgttc aagcttttaa gcttagtatt tttctctggt ttgctttttt tcggagctgt
     8581 atcgttccta tatcgccata tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt
     8641 aaataatttg tatcactttt cgtggggagt tggcggaata acaattgttg tgcttattgt
     8701 ttttcttcta cttatttggc tccgttacca tcaattgtcg cgtcatttcg atcaatggct
     8761 tagtcaaaga gcacaacagt ttttacataa taaaccaact tctcaagatg atgactatct
     8821 ttattttgag aagacttcga cgagtcaagc ttttcggata caaaaaagtg attgccagtt
     8881 attgctgaga tcacttgatg atttacctat ctacttaggc gaaagcaaat caaaattcgg
     8941 aatgaacaca ttccatattt ttttactaga aaacgaaccc agatgggaaa aaagccagtc
     9001 ttcgcctggt ggatacctga caactaagat gagtacatta gtcactggtt ttttcctcat
     9061 tcttttagct ggaacaatct attatgggag atacactcag ccaaaaatgt cttctcatat
     9121 aaatgctgct tctcacatga caatgaactc aactaacgat acgcatatac aaaagacaaa
     9181 tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa agataaatga
     9241 tctcaaactt gatacagaga cgcactctct ctatatgaca acagatagtg gtgctaattg
     9301 ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc gaatacaccc tgacaacggg
     9361 gctgattcct gaaggttatt ggatggacaa cacatatgat atttccgccg atttctcatg
     9421 gtttatctat tcttctgggg atgatgtacg tctgctgact tcaagagata atggtaaaac
     9481 atggcaaacg tctattatca cccaatcagt tcagccgata cgttatcgaa aagcggtatt
     9541 ttatggcaac gatggtctat ttatctgtac atcagccagt gggatgtctg cagaaacttt
     9601 gtacatttac actactatgg atggcggaat cacttggata aaaagtggaa gtacaatgat
     9661 caatcagcca gtacaaaatg tcagcttcgt tacaacttct ttaggttttg tttcgacacg
     9721 tgaaagtttg ttttatacaa acaacggagg acgctcattc aaagaatcag ttctgtcgat
     9781 tccggaaaac tatcagacag gggggataga tctttttcaa tcaccaaatg aagttactca
     9841 aataagcgcg aatacgttag agacaaaatt ttatttgttg aaaaaaggac caatcgatgc
     9901 agggaaaatg tttgcctgcc tcttccgttc aactgataac ggggaaacat ggcaattcgt
     9961 tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga gagattgtga gcctgacgtt
    10021 cagcaccgag tcacaagaaa caaattcaaa aaacagcttc tcatatttgt tgaatagtgt
    10081 aactcgtgat tcggagttga caagagcaac aagaagcgat tttgaagtat agcttctcgt
    10141 attttacaag atcgtgtctt attgccgata ggttgtctct tgaacaccgt ttattcgtaa
    10201 aaagggattg tgacaagtct tttgtcacta cttaccgaac gaaactgaag gagctatcca
    10261 aaaagtatca ttagtattat tctttgtgtt tctagtggca ccactagtac atttaaaaaa
    10321 gctatcgatc atggactaat catacatgat cgatagcttt ttagataatg tttgttacgc
    10381 tatctgaaat tgactattat aaagactggc ataaagcgtt ggtgattgga gtagttcaga
    10441 atgtgttcct ttttcgacga tatcgccatt cttcattaca aggatcatgt ctgcattttc
    10501 aatcgttgac aaacggtgtg caatcacaaa acttgtccta tcttttgtca cagcttccat
    10561 tgcttgctga atcaatgctt ctgttcgtgt atctacgcta gaagttgctt catctaaaat
    10621 aacgacagga ggattcgcta gaatgactcg agcaattgtc aataattgtt gctgtccttg
    10681 agataacgtg ccgttttcgc tagaaatgat cgtatcatat ccgtctggca gtgttcgaat
    10741 aaagtgatca cattgtgcga tttcagccgc ttcgataatt tgttctcttg tggcatccat
    10801 tttcccgtag gcgatattat cagctaccgt tccttcaaac agccaggtat tttgtaaaac
    10861 catcccaaaa gaacgacgta gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa
    10921 ttcgatcttt cctttattca gttcataaaa acgcatcagc agattgacta aagtcgtttt
    10981 tcctgcacca gttggaccaa cgatcgctac cgtttgtttt ggctttactg aaaaatcgac
    11041 ttgattcatc aatattttat ctgatgtgta accaaactgt acatttttga aagctatttt
    11101 tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc
    11161 taatatctca aatatcctat cgattgctgc cattgcggat tggatcgagt tgatcacata
    11221 agaagcggta gaaattggtt cagaaatctg attgatgtac tgaagataag cttgtaagaa
    11281 gccgatcgtc acaccacctg atagaacaag catagcaccg aataccgcac ttatgatgaa
    11341 ggcgagttga ttaatgaaac gaatggccgg atagatagca cagttcataa attgagcacg
    11401 tttaaaagcg ttgtattgct ttttatttac ttcgtgtata gcagcttctg catgagcttg
    11461 ctgattgaat gttttgacca ctagattacc agctaagatt tcttccattc gattatttaa
    11521 aacacccagt tcatactgat tctgttcaga aatctgtttg tttttgttcg caatcttagt
    11581 tgtaacaatt gaactgccaa cgatcagcaa taagacgagc aaagtaagct tgctttcgat
    11641 ataaaacagc atcagaccag cagcagctat tgttacgaca gaagtaaaaa attgattgat
    11701 acctgtcaat aacacttgag aaagttgatt gagaccggta gttgtccgac taatgatgtc
    11761 acctacttga tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc
    11821 ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc attgtccgtt cctgaataaa
    11881 tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat aagacaggga acaacaatgc
    11941 ttgtttgata tcactgaaag aaagctgtcc aatacctgtt tttcttattt ctgataagag
    12001 atcatcgatc ccgattccca tgatcaaggg catggctacg actaataggt tcccgaataa
    12061 actgcataga aacaatccgt aaaaatattt atgttcaggt ttgatcattt tccaaaaacg
    12121 tatcaaagca cgcgtgttca acgttttttt cttttccatc gtatcccttc tttctatatt
    12181 ccttgtgatt ttgcaaactc ctgataagtt tggcaattag acagtagttc ttcatgagta
    12241 ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt tatctgcttg ttggattgta
    12301 ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca tgtgtttatg aagggctgct
    12361 cttaatgctg catctgtttt atagtctaaa gcggagaaac tatcatcgaa tacatagatc
    12421 tctgctggtt tgatcaacgc tcgagcaata catatccgct gtttctggcc ccctgaataa
    12481 ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc cactagttct
    12541 tctaattgag cgatcttgat cgctttagac aactcttctg ttgaggcata ttcatttccc
    12601 atctgcaagt tgcttaaaat tgtcccgctg aataagaaag ccttctgagg gacataactg
    12661 atgtgggagc ggatctgttc ttgagtcatt tgagtaagag gaataccacc aagggttact
    12721 tttcctatcg tagggtcact aagtctcaag agaagtttcg ccaaactact ttttccagat
    12781 ccggtcccgc caacgatcgc tgttgttttt cctttaggta ttgtgaaatc aatatcttct
    12841 agaactggat cagagatttc agtatattta aatgtgacat gttcaaaagc agctgcattt
    12901 gtctgatcaa aggttggttc agctactgtt ttgatcgtct cttcctcaat ttcgatctct
    12961 gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg atttagggat aatgaccaat
    13021 accatagctg cgatcatcaa gtaagcaagt gtcagcatag agtattcgac tacagcagtc
    13081 acgacaccga tttgtaatgc tccttctgct acaagatttc cgccaaacca caaaatagaa
    13141 gcataaacga ttcccatgag caaaaaagca attggagtga cccaagcaaa tattcggttc
    13201 actttgatca tttgattagc atagtcagca aagctcttat tcgttcgctt ttcttcatat
    13261 gattgattat caaaggcacg gatcatatta atcccagtaa agaattctct caatgtgatg
    13321 gtgatccgat ccatttttgg ctggatactc aaagataaag gtgcccctcg tttcatcagt
    13381 cgataaacaa tccatacata aaaaacaaca gagatcaatg taacccatgt tagtaataga
    13441 gaataacgaa acatcatgag aatggtgaaa acgcaaataa ccggtgctgg taagatcatt
    13501 tggcaaaata aaacaatcat ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa
    13561 agagatccag aactaaaacg actgatatcc ttcattgaca gctgttggaa tttacggaaa
    13621 tatttctccc gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa gtaactagag
    13681 gcaactgcag cgattgttcc tagaaacgct acaagcagca tttcaactcc aatttttttt
    13741 ataactgagg cttcgccact tgctatacct gtgtcgatca gctttgctac taattttggt
    13801 acacctaatg taccggcgat ttctaaacag atcatcacaa aggtaaggag agcgagtcct
    13861 ttgtttttct gtaagaatgt ttttaagata gacatattat tcctcctttt ttcctgatac
    13921 ttttcgattc tataccttca agtaacttga aggtcaatag aaaaaagaat aaactgaaaa
    13981 agaggtgaga aaaaatgaaa aaaatgtata cgattggcca agtatcggaa ctatttgatt
    14041 tgccaaaatc aacgatccgc tattgggatg aacaaggatt aatccgttca agtagacaag
    14101 aagagaatga ttatcgattg tttgatatcg atgatatttt tatgctttat gatatcgttt
    14161 tttatagaaa attagatata ccgatcaaac agatgaagaa tttatatgga aaaacattga
    14221 cggagttata tgagacactt gatgaaacag aaagaagaat ccataaagaa ctggtggtga
    14281 tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca gttgaaattg atgatcgata
    14341 caaacgagga agaatttcct gtagaagaga tcccttttga ctgtatgatc tcaacggagt
    14401 ttgaagatat tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt gggatgatga
    14461 gtatgccagc ttccagttca cagacgatgt atggattttt tatcgaccct agtgaagtac
    14521 atttatttac tcaagacgtg atttgggaga aaaaagatac ggctgtttat cgtcgattct
    14581 tattgaaatc cgagatgaac catgcggaac gtaataatat tttagaaatc agagaaagaa
    14641 tgtatgaaaa aggatggaaa acaggagaag tgatcggcca gtatctattg acgaatacgg
    14701 atgaaaacaa tatcagaact gaatattatc atgcatggat agaaatgaaa aaataggaaa
    14761 tatgtgttta agataactcg aaaatgatgg ttgttttagt tataaagaaa aattttctgg
    14821 ttcgattacg ggtttaatgt gggggattat tagtcgtgga tagatagaat tttagttttt
    14881 tagaggaaat atgtctttaa aaatggaatg gttgcaattc ttagcgatat atacgatgta
    14941 attgtcctct taaaaaagta tggaaaaata attgtattgg gtgttatgta taattcatgg
    15001 tgaactgaga ctaatgagaa gaaaaattca tattgttatg agtgagattg tatcagatga
    15061 gaatgataaa ttaaataggt gcataaaagc attacactta aaatacattg ttgcttacat
    15121 tattacaata tgttaataag gcaaaaataa atagatggat tgaataaaca tattttttaa
    15181 tcaaatagtc cctactgtga ctttcctcag ctatcggtga tactaccgat agcatttttt
    15241 tgcttataaa ataattataa gtatttagag atttttcttc taacattctt caaacaagaa
    15301 aaatcacaaa ggttattgtt atttttatat attcttttta agaatgattg tttttttata
    15361 gaaattttaa aaaataaaac acgttattga tggatagtta gtttgttatt tggtaatatt
    15421 atagaagttg actggtagtg atttattttt cgtcgtttta taaaaatgtt attttttgga
    15481 aaaattcaca aatatgtaac gaatgttttt ctggaatttg ctgtgttaaa aagcagtatg
    15541 actaatataa atactattgt catttatgca attttttatg gatagtaatt ttagagggga
    15601 atcaatgaag gaagaataga agggaaaaat agttgcaaat acgccagtta gcaaaaatag
    15661 caatacatct tcaagcttta aaattcgaga gaatcatctt ggtagtggaa tcaactacaa
    15721 caaaaggatt aaaccaatta atagatagaa ttaccaatgg aaaaatagaa aaggtagtta
    15781 ttctatacaa aagttgttta attcgcttcg gttatgaact tagtgaaacc ctttgcaata
    15841 tatggtacag aatatagaaa ttattgacat tacagaaaat gatgaagatt taatacagat
    15901 tgtcactatt tgtagatacc ttttacaagg aaaccgagcc aataaagcta agaagatgat
    15961 taaggagttg atggaagacg atacaagtta agaaagtgaa actacgccca aataaagagc
    16021 aaaaagagta attggacgtg gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt
    16081 attagtaatg acggtttgag aacatccatg acgagaatga aggaacatcc taaatgcgtt
    16141 tagttgaacc aagtgccaaa taatatggcc aaataagcga tcagttatcg atgaatccca
    16201 agtattctaa actatatatt cattttgatg ctaagcattg atatattttt ttgggattga
    16261 tgaaggttaa ccaatacagg gactcactga tgaaattcta gagtctgacc ttggtatcaa
    16321 cgatttagtg attgtttcta atggaaaagt ataaaagtat ataagtacat taacaaaata
    16381 actattaggg ttacgctcca gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata
    16441 attatggtta agccacacgg acaactaaaa gtcatacaaa gctatcgaat caatcaacag
    16501 aagacaatcg gcaatcatta gctttgttta cagcttacat tgcaacgctt gagttgaacc
    16561 gaaaatacga gggaggtaac gcagccattt tgagttgggg aatcttttta gaaaattatc
    16621 gggtaccttg ttgtttagtc aaaattataa gggggagtaa tagtgaggaa caaatttcat
    16681 aaactgttaa gtatgtttct agttatgctt attgtgatca atcaatttgg gaacataacg
    16741 gttctagctg agtctttagc agaacagcca gagcgttcgc tgacgatcga agttgatcaa
    16801 gaggaggctg ctttgggtga gcagttccag ttgcgagtca gaggaacacc agacctgcta
    16861 aatgcattgg tcattgataa gagtgaggat ttgattgaag gggaagtgaa gcaactcaat
    16921 gattacgaac gtctgattac ttattcttcc cagcattcag gtaaatacga attgaccgca
    16981 agaagcggtg atattcaatc aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc
    17041 acaagtgagt cttttaatgt accagaagaa agcacaagtg aatcttttaa tataccagaa
    17101 gaaagcttaa gtgaatcttt taatgaagcc agatcatctg agccatctat aacgcaggaa
    17161 tctcagattg aaagacaaga ggagctcttg aattatgaca tcggcatctc tgctgagcgc
    17221 cccactttgc cgagcaggca tacacaaacg ttaaagggat acattcaagg aggcgctggc
    17281 gatattcttg aagaatatgg ggagatggtc gtacgagttc ctagagcatc gattggctta
    17341 attgagttca ttagagtgga ggtcgctgat gattcacctt taaagccact ggcattttac
    17401 caagaaggca cagacgttgt ttttccattt acctttgatt atgaaggatt aaatgaagcc
    17461 gctcagtcgt atacattcag tatcacgttt gaagttgctt tctttcaatc aggcggtact
    17521 gagccaaata atgactttca agtgagtctt acttatgctg agcgagcagc aaacacggaa
    17581 ttttcaatta cgcctattcc ttcatcaaga cccatttttt cgaaattata ctatggctct
    17641 agagaaatcg acagccaagg aaatcttggg aatctgctga ttgacgggca agacgtgacc
    17701 aacagtaata aatttatttt gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg
    17761 gttaccgatg tactaccgca aggaacaact ttggcaccga atccgactac atggagttcc
    17821 actggaaata gagaacctat ttcaggtggg gttcggatcg cagaagtaac gaatccaggt
    17881 gaagcaaacg ttagaactca ttatgtttcc gaggaatttg tggacaaagt atcttttaat
    17941 aaggaaacga atacacttgt tgtggacttt ggtaatctag cagagggtca atcgtatttt
    18001 attgaatatg ctcttgaagt gaccgatgtc gacttgttca atgaaggaag acaagaggat
    18061 gggttttctc ggaatcgagc gagtatgacc tacaataatc aggtaatgag ccgagactac
    18121 cgcttgagaa tccctgaaag taatcttccg gcaatctctt taagaaaaag tgtggacaaa
    18181 aatgttatca atctcaatga aaatgaacta acgtatactc tgcatgtctc gatgttttct
    18241 ggaacaagaa atacgcttgt tattgaagat gttttggaag aaggcatgga atttgtgtca
    18301 tttctatcag atattccctc tttcattgat gccgaagtca ataatggaat agttcgtttc
    18361 agtgcgaata atacgattgg tggtccgaca agttttaccc tctcttttaa ggtcaatgta
    18421 gaggcgtatg cggtcggcga tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat
    18481 cgattgaata cgagtactgt aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa
    18541 actgatgaga atggccaaag gctggcagga gcaaaatttg aaattttatc aagcgattca
    18601 caagtgatac aagaaggtat aacggcagaa aacggagaat ttttaagtga accgcttcct
    18661 gtaggtagct atcaagtccg agaaacagaa gcaccggaag ggtatctttt agatgcgacg
    18721 gtacatgatg tttttataaa tgaatactcc atagaagaag tgacgttaac cattgaaaac
    18781 atccttgata cgggtggtgt tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt
    18841 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggt
//
LOCUS       DACCCM010000058        17107 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCCM010000058 DACCCM010000000
VERSION     DACCCM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.061"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            7..1398
                     /locus_tag="IVK78_001964"
     CDS             7..1398
                     /locus_tag="IVK78_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6375417.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IVK78_001965"
     CDS             1414..2454
                     /locus_tag="IVK78_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6375418.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IVK78_001966"
     CDS             2473..3558
                     /locus_tag="IVK78_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP6375419.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="IVK78_001967"
     CDS             3591..4553
                     /locus_tag="IVK78_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6375420.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="IVK78_001968"
     CDS             4546..5979
                     /locus_tag="IVK78_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP6375421.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="IVK78_001969"
     CDS             5976..7046
                     /locus_tag="IVK78_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375422.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="IVK78_001970"
     CDS             7033..8454
                     /locus_tag="IVK78_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6375423.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="IVK78_001971"
     CDS             8467..9474
                     /locus_tag="IVK78_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6375424.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="IVK78_001972"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="IVK78_001972"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP6375425.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="IVK78_001973"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="IVK78_001973"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP6375426.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="IVK78_001974"
     CDS             11608..12285
                     /locus_tag="IVK78_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6375427.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="IVK78_001975"
     CDS             12275..13417
                     /locus_tag="IVK78_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP6375428.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="IVK78_001976"
     CDS             13430..14407
                     /locus_tag="IVK78_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6375429.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="IVK78_001977"
     CDS             14407..15315
                     /locus_tag="IVK78_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6375430.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="IVK78_001978"
     CDS             15316..16068
                     /locus_tag="IVK78_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP6375431.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="IVK78_001979"
     CDS             16073..17020
                     /locus_tag="IVK78_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6375432.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2190 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DACCCM010000059        17008 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCCM010000059 DACCCM010000000
VERSION     DACCCM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.062"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..127)
                     /locus_tag="IVK78_001980"
     CDS             complement(<1..127)
                     /locus_tag="IVK78_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP6375433.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="IVK78_001981"
     CDS             complement(231..542)
                     /locus_tag="IVK78_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6375434.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="IVK78_001982"
     CDS             complement(544..741)
                     /locus_tag="IVK78_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP6375435.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="IVK78_001983"
     CDS             complement(1135..2862)
                     /locus_tag="IVK78_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6375436.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="IVK78_001984"
     CDS             complement(2855..4048)
                     /locus_tag="IVK78_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6375437.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="IVK78_001985"
     CDS             complement(4235..4879)
                     /locus_tag="IVK78_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6375438.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="IVK78_001986"
     CDS             complement(4973..5107)
                     /locus_tag="IVK78_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP6375439.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IVK78_001987"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IVK78_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP6375440.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="IVK78_001988"
     CDS             complement(7238..7711)
                     /locus_tag="IVK78_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP6375441.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="IVK78_001989"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="IVK78_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP6375442.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="IVK78_001990"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="IVK78_001990"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP6375443.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="IVK78_001991"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="IVK78_001991"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP6375444.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="IVK78_001992"
     CDS             complement(11758..12897)
                     /locus_tag="IVK78_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6375445.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="IVK78_001993"
     CDS             complement(13206..13919)
                     /locus_tag="IVK78_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP6375446.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IVK78_001994"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IVK78_001994"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP6375447.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="IVK78_001995"
     CDS             15108..16640
                     /locus_tag="IVK78_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6375448.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="IVK78_001996"
     CDS             complement(16831..>17008)
                     /locus_tag="IVK78_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375449.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5280 a         3494 c         2910 g         5324 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gaaaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DACCCM010000060        16818 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCCM010000060 DACCCM010000000
VERSION     DACCCM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.063"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..454)
                     /locus_tag="IVK78_001997"
     CDS             complement(<1..454)
                     /locus_tag="IVK78_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6375450.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(559..1422)
                     /locus_tag="IVK78_001998"
     CDS             complement(559..1422)
                     /locus_tag="IVK78_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6375451.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1618..3717)
                     /locus_tag="IVK78_001999"
     CDS             complement(1618..3717)
                     /locus_tag="IVK78_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6375452.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IVK78_002000"
     CDS             complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IVK78_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP6375453.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5070..6911)
                     /locus_tag="IVK78_002001"
     CDS             complement(5070..6911)
                     /locus_tag="IVK78_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP6375454.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7093..9486)
                     /locus_tag="IVK78_002002"
     CDS             complement(7093..9486)
                     /locus_tag="IVK78_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6375455.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9593..10453)
                     /locus_tag="IVK78_002003"
     CDS             complement(9593..10453)
                     /locus_tag="IVK78_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP6375456.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10512..11033)
                     /locus_tag="IVK78_002004"
     CDS             complement(10512..11033)
                     /locus_tag="IVK78_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP6375457.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11353..12072
                     /locus_tag="IVK78_002005"
     CDS             11353..12072
                     /locus_tag="IVK78_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6375458.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12098..14689
                     /gene="mprF"
                     /locus_tag="IVK78_002006"
     CDS             12098..14689
                     /gene="mprF"
                     /locus_tag="IVK78_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6375459.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IVK78_002007"
     CDS             complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IVK78_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6375460.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16432..16788
                     /locus_tag="IVK78_002008"
     CDS             16432..16788
                     /locus_tag="IVK78_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6375461.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5765 t
ORIGIN      
        1 tttctttttc atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta
       61 aaaaatgata acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt
      121 cctcgatcgt gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt
      181 ttctcaaatc ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt
      241 ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt
      301 tcactaaaaa gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa
      361 gcagagtagc ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg
      421 tttttttcat cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata
      481 attacaaata aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga
      541 aaaacatgca agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg
      601 tgtaatcaat ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat
      661 accaaaagct ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc
      721 tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc
      781 tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt
      841 tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata
      901 gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac
      961 atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc
     1021 tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc
     1081 ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc
     1141 tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat
     1201 tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg
     1261 tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga
     1321 tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc
     1381 aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag
     1441 tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct acttgaatag
     1501 ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc
     1561 tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta
     1621 attgctccca tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg
     1681 caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt
     1741 ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat
     1801 atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca
     1861 tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc
     1921 tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc
     1981 agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc
     2041 agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc
     2101 agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt
     2161 tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac
     2221 tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata
     2281 ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt
     2341 ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc
     2401 gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg
     2461 gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa
     2521 atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg
     2581 atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt
     2641 tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc
     2701 tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt
     2761 ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc
     2821 tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt
     2881 ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga
     2941 ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt
     3001 tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac
     3061 ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt
     3121 ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt
     3181 ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt
     3241 atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac
     3301 gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt
     3361 ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat
     3421 attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa
     3481 ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa
     3541 ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt
     3601 tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc
     3661 tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac
     3721 ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt
     3781 cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata
     3841 gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca
     3901 gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg
     3961 ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg
     4021 ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc
     4081 taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc
     4141 ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg
     4201 gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga
     4261 aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc
     4321 cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat
     4381 acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg
     4441 gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt
     4501 aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg
     4561 gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa
     4621 acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa
     4681 tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata
     4741 catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc
     4801 ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc
     4861 caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt
     4921 caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg
     4981 tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt
     5041 tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa
     5101 ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct
     5161 tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct
     5221 agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc
     5281 accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat
     5341 tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga
     5401 ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga
     5461 tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt
     5521 tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt
     5581 gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt
     5641 gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact
     5701 agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt
     5761 aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa
     5821 gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg
     5881 acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca
     5941 aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt
     6001 tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc
     6061 tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg
     6121 atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt
     6181 tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag
     6241 cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga
     6301 taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac
     6361 aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt
     6421 gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat
     6481 tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct
     6541 ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac agctatagta
     6601 cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct
     6661 tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca
     6721 caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct
     6781 tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt
     6841 ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc
     6901 cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat
     6961 ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca
     7021 caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta
     7081 tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca
     7141 gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc
     7201 tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc
     7261 tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac
     7321 aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt
     7381 tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa
     7441 ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt
     7501 atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt
     7561 gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat
     7621 aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac
     7681 tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag
     7741 attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc
     7801 tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc
     7861 tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata
     7921 gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc
     7981 atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc
     8041 agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt
     8101 ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc
     8161 ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg
     8221 ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac
     8281 gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc
     8341 gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat
     8401 agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt
     8461 ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt
     8521 ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc
     8581 ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat
     8641 tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga
     8701 aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg
     8761 aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg
     8821 aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac
     8881 tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc
     8941 tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc
     9001 ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa
     9061 atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag
     9121 tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg
     9181 ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc
     9241 acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat
     9301 attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg
     9361 gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc
     9421 cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa
     9481 aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg
     9541 ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat
     9601 attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag
     9661 gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg
     9721 ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa
     9781 tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca
     9841 caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat
     9901 acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc
     9961 cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgt
    10021 ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat
    10081 ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg atacatccga
    10141 caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa
    10201 gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt
    10261 tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag
    10321 ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata aacattccct
    10381 tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt
    10441 gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat gattgaacat
    10501 ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc attcattttg
    10561 acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc
    10621 aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc
    10681 agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt
    10741 acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat
    10801 ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg
    10861 tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga
    10921 tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt
    10981 tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt
    11041 accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc
    11101 atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat
    11161 tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt
    11221 ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga
    11281 tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca
    11341 gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt
    11401 tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc
    11461 ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta
    11521 gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg
    11581 gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga
    11641 tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat
    11701 cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc
    11761 atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc
    11821 aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta
    11881 aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa
    11941 cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa
    12001 ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt
    12061 tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg
    12121 gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat
    12181 tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt
    12241 tgccactata cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt
    12301 acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg
    12361 ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg
    12421 gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt
    12481 gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct
    12541 gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat
    12601 atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa
    12661 gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt
    12721 gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg
    12781 gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc
    12841 tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat
    12901 tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat
    12961 cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca
    13021 gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt
    13081 cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac
    13141 agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc
    13201 tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa
    13261 acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag
    13321 tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc
    13381 taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg
    13441 aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga
    13501 tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg
    13561 gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt
    13621 ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat
    13681 ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat
    13741 gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa
    13801 taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga
    13861 agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga
    13921 agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc
    13981 ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt
    14041 cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac
    14101 tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa
    14161 agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt
    14221 caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa
    14281 taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat
    14341 ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa
    14401 tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag
    14461 gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga
    14521 atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag
    14581 ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac
    14641 acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac
    14701 cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct
    14761 tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt
    14821 ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat
    14881 gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat
    14941 gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca
    15001 ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg
    15061 cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct
    15121 tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta
    15181 gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg
    15241 ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact
    15301 gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca
    15361 ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc
    15421 actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact
    15481 gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat
    15541 gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg
    15601 cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc
    15661 aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt
    15721 tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc
    15781 cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg
    15841 cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct
    15901 tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat
    15961 actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca
    16021 atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct
    16081 aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga
    16141 ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata
    16201 cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac
    16261 cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct
    16321 tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt
    16381 caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa
    16441 ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat
    16501 tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct
    16561 acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat
    16621 aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg
    16681 aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata
    16741 aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta
    16801 aaattttatt agataaaa
//
LOCUS       DACCCM010000061        16527 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCCM010000061 DACCCM010000000
VERSION     DACCCM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.065"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(142..417)
                     /locus_tag="IVK78_002009"
     CDS             complement(142..417)
                     /locus_tag="IVK78_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP6375462.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IVK78_002010"
     CDS             complement(660..1970)
                     /locus_tag="IVK78_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP6375463.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IVK78_002011"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IVK78_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP6375464.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IVK78_002012"
     CDS             complement(3516..4196)
                     /locus_tag="IVK78_002012"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP6375465.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IVK78_002013"
     CDS             complement(4245..4880)
                     /locus_tag="IVK78_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6375466.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IVK78_002014"
     CDS             complement(4946..6349)
                     /locus_tag="IVK78_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP6375467.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IVK78_002015"
     CDS             complement(6336..7367)
                     /locus_tag="IVK78_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375468.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IVK78_002016"
     CDS             7398..7619
                     /locus_tag="IVK78_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP6375469.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IVK78_002017"
     CDS             complement(7784..8521)
                     /locus_tag="IVK78_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP6375470.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IVK78_002018"
     CDS             complement(8674..10014)
                     /locus_tag="IVK78_002018"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP6375471.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IVK78_002019"
     CDS             complement(10251..11774)
                     /locus_tag="IVK78_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP6375472.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IVK78_002020"
     CDS             complement(12152..12763)
                     /locus_tag="IVK78_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6375473.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IVK78_002021"
     CDS             complement(13093..13809)
                     /locus_tag="IVK78_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6375474.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IVK78_002022"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IVK78_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP6375475.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IVK78_002023"
     CDS             complement(14368..15165)
                     /locus_tag="IVK78_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP6375476.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IVK78_002024"
     CDS             complement(15135..15506)
                     /locus_tag="IVK78_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6375477.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IVK78_002025"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IVK78_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP6375478.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3576 c         2798 g         5508 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacttgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttcttt
//
LOCUS       DACCCM010000062        16509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCCM010000062 DACCCM010000000
VERSION     DACCCM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.066"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            184..1182
                     /locus_tag="IVK78_002026"
     CDS             184..1182
                     /locus_tag="IVK78_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6375479.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="IVK78_002027"
     CDS             1294..1803
                     /locus_tag="IVK78_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP6375480.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="IVK78_002028"
     CDS             complement(1833..2492)
                     /locus_tag="IVK78_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP6375481.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="IVK78_002029"
     CDS             complement(2494..3258)
                     /locus_tag="IVK78_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP6375482.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="IVK78_002030"
     CDS             complement(3266..3943)
                     /locus_tag="IVK78_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP6375483.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="IVK78_002031"
     CDS             complement(3980..5365)
                     /locus_tag="IVK78_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP6375484.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="IVK78_002032"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVK78_002032"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IVK78_002033"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IVK78_002033"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP6375485.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="IVK78_002034"
     CDS             6304..7161
                     /locus_tag="IVK78_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP6375486.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="IVK78_002035"
     CDS             complement(7242..7577)
                     /locus_tag="IVK78_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375487.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="IVK78_002036"
     CDS             complement(7644..9014)
                     /locus_tag="IVK78_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP6375488.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="IVK78_002037"
     CDS             complement(9092..9628)
                     /locus_tag="IVK78_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6375489.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="IVK78_002038"
     CDS             9937..11418
                     /locus_tag="IVK78_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6375490.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11521..12228)
                     /locus_tag="IVK78_002039"
     CDS             complement(11521..12228)
                     /locus_tag="IVK78_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP6375491.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12307..12645)
                     /locus_tag="IVK78_002040"
     CDS             complement(12307..12645)
                     /locus_tag="IVK78_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP6375492.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12756..13718)
                     /locus_tag="IVK78_002041"
     CDS             complement(12756..13718)
                     /locus_tag="IVK78_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP6375493.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLVSGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13733..15064)
                     /locus_tag="IVK78_002042"
     CDS             complement(13733..15064)
                     /locus_tag="IVK78_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6375494.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15072..15752)
                     /locus_tag="IVK78_002043"
     CDS             complement(15072..15752)
                     /locus_tag="IVK78_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP6375495.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15971..16450)
                     /locus_tag="IVK78_002044"
     CDS             complement(15971..16450)
                     /locus_tag="IVK78_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP6375496.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         4885 a         3441 c         2736 g         5447 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccaaat aaatggtatt
    11461 caatcagcta actctcggta ttagctcaga tcaaagcgct gatttcacaa cttctttatt
    11521 ttaatctctt cggttgccgc cgaacaataa gatcaagcgc aacagctgca ggaatgtcga
    11581 aagcgctgct gcaacataag tcagtgctgc tgcaaataat acgtgtcttg ccatcggtac
    11641 ttcttcttcc gtcagtagtc cgccttcact caaaatttgg attgctcgcc gagacgcgtt
    11701 aaattcgact ggtaatgtca ccaattggaa aagtaatgct aaagaaaaag cgaaaatccc
    11761 gatattgatt aacgtagagt tccagctaag caatacacct accataatca aggggaacga
    11821 aatcatcgaa ccaaagttag ctgctggaac aagtgctgca cgaatcttca atgggacata
    11881 acccgaagca tcttgtaccg catgtccgca ttcatgagct gctacaccta tcgcagcaac
    11941 tgaagtagac tgtgcagttg cttgagacaa actcaacatc ttattacctg aattgtagtt
    12001 atctgtgaga tctccggcaa tttgttgaac accgacatta tttatctgtt gactttgtaa
    12061 tatatattgt gctgcttgtg taccagtcac attgtttttg cttctcactt gatcgtattt
    12121 tcggaaagta ctattgacgt acgctgacgc aattccggaa ataactaatc caagaatgac
    12181 caaaagaaat gtcggatcta aaaaaccata cattccataa ccatacattt tcgtttcact
    12241 ccttttcgtt tactttattt tatcattagt agtaggaaaa gaatgtgaaa agaccttgaa
    12301 tattctttaa ttctttttca caaattccga tttcagtttc atcggtccaa atccttctac
    12361 cttgcaatcg atattatgat cgccttctac taaacggatg ttcttgactt ttgttccttg
    12421 tttaatggca cctgacgcac cttttacttt caagtcttta atcactgtca cgctatcgcc
    12481 ctctaacaga cggtttccat ttgcatctaa taccactaat ccgtcttcta tgacagtttc
    12541 ttgttcttct ttgttccatt cgttcccgca ttctgggcaa atcaacaagc ctcgatcttc
    12601 atatgtgtac tctgagccac attctgggca atttggtaat gtcatcttct ttctccttta
    12661 aaatctttag tgagcactat tgtaacggaa aaagaagatc ttgaaaagat acatttgtga
    12721 tttttactac aaaaggcacg ttttcgaacg aagtatcatt ctgtttttct gctcttatac
    12781 gattggcata tatcttggat ctctttatag atttcctcta cttctgctaa atcttttgca
    12841 ttcgcaccac ttactaatgg atggcctcct ccatgatggc gtttagccaa ttcattgatg
    12901 atcggcccct tagaacgtaa acgtacgcgg taataccctg aaggctgctg aacaaaaatc
    12961 ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa cgatcgaagc tgtctctgaa
    13021 tcatcgattc catatttatc aagaatttct tgcggaagga ttattcttcc tgcaccattt
    13081 tcatccacct ggatattttg ataaagataa ccggaaagtt tagcgacttt catcggcatt
    13141 tgttcaatct ctctgttcaa agcacttgca tcaaatggat aatttctcaa tatcgaagca
    13201 atctctaatg tacgggacgt cgtggaggga tacaaaaagc gccctgtatc tccgacgatt
    13261 cctgcataaa gtaaccgtgc agccttaacg ttcatcttta aagattcagg aaacatcaaa
    13321 gcaaattctg caatcatctc actacagcta cttgcttttg tatttaccca cacaagatct
    13381 ccataagggt catcattggg atgatggtcg attttgatca gtttttctgc caactgatat
    13441 ctttgatcac tgattctggg tgaattagct gtatcagtca cgatgactag cgctccttgg
    13501 tagaagacat cttcaacttc ttgcatttct gctaaaaagc gaagcccttc aatcgtttca
    13561 cctacttgac gtatttcttt ttctgggaaa ctttcccgta atagttcggc taatcctact
    13621 tgtgatccta atgcatcagg atcagggcgc aaatgcctgt ggataatgat tttatcgtat
    13681 ttttttattg tatctactat ttcttgctgt actgtcattt cactcctgct ttctaagaac
    13741 gttccattac ttgacaaaca acgattgctt tggaaacgat ggtgttttca ataaacactt
    13801 cgatatccag tttagcggaa cgtctgccga tttccaatac acgtggacga atgtctaatt
    13861 cgctttccaa ttggatcagc cgcaaataat gtaggttcac ttgttcaatc aagatatttc
    13921 gtttttggtt catcaccatt gttttttgtg cgacatcaga aatgatttcg ctaagtacgc
    13981 caaaagaaat ggttccaacg ccattgacca tttgtggtgc tactacgaat ttgaacttag
    14041 ggttttccaa acgatttcct tcacggtcag tatcaatcgt atggatattt ccagatatct
    14101 gatccgaaat cgtatctgca atctgcggtt gtcgctggac aagctgcatc gctttcatta
    14161 catcttgccg ggtaatcaag ccttccaatg tcaagtcatc ggaaacgacc ggcatgactt
    14221 ctagcccatc ccagatcatt tgatggctaa cagaggcaat actcattgtt ttctttacac
    14281 tgattggttc tttcgtcatc acccgatcga tgatctggtt tggcgatttc cctaaaacat
    14341 cttttgccgt aacaatcccc atcaaacgat gatggcggtt gacgactgga aaacgcgaat
    14401 gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt atctgtctgc tgcaaataat
    14461 gagtcttttc agctggcata tagatatcac tgacaagcat gatatctttt ttgatcaatt
    14521 ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa tgtatcataa gaggtgcgta
    14581 agatcggcat ttcaagatca tcagctagct ttgcaatctc agcagtcgta ttgaatcctc
    14641 ctgtgatcaa tactgcggcg ccatcttcca atgctaattt ttggacttcc gcccggttgc
    14701 cgacgatcat tagtgatcct ggcgtaatat agcgttccat ggcatgttta gtcatcgcgc
    14761 cgatgacgaa tttattcaag accttgtcta atcctgccgc cccgcctaaa acgtcccctt
    14821 caatgatcct gacgacctcg ccaaatgtca aacgttcaat atgttttttt aatttgcgtt
    14881 cgatacggat ggtccctact cgttgaattg ttgaaactag tccgacattt tctgcatctt
    14941 tgattgcacg gtacgctgtt ccttcactga cattcaattc ttttgcgatg cttcttacag
    15001 aaattcgatt gccaacaggt aaactttcaa tatattctaa aatcaaatca tgttttgtcg
    15061 ccattttctg cctacacttc cagcgtttcc gagacgctca ttatttttcc ttcactatct
    15121 tccagtaact ctacaaaaga ttcgggatct tgttcaatga cagggaaagt attgtaatgg
    15181 attggaacta ctttcttggc acgaattgtt tgcgcagcct ttgctgcatc ttctggcccc
    15241 atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa tatcgaattc ttctgcaagc
    15301 aatcctagat cactgaaata agcagtatcc cctgcatgat aaatcgtttt tccttctgct
    15361 tgaataataa ttccgctagg ctcgcccata tactgaacga taccgtctac ttcatagctt
    15421 gaactatgct gtgcatgtac gaattttacc acgccgaatg gaaacgcatg ttttccaccg
    15481 atattcatac cgaatgaatc tactcctctg ctgttcgcat aatcagcaat ctcaacaata
    15541 ctgataattg tggcttgatt ttcttttgca ataggaatca tatctcctat gtggtcacta
    15601 tgaccatgag taatcagcaa ccaatctgtc tttacttctt ctgcagttaa gtctgtcaat
    15661 ggatttcctg taataaacgg gtcaatcatc acgcgttggc cattttccat ttcaattgtt
    15721 aaacaggaat gtccatgata cgtaattttc atattttaca cctcgcgttt tctctgtatc
    15781 ttttttatta aaaacgattt catcacttgc acatcaatga aaaaatatag ctgctaaata
    15841 tattttcatt ggattgatag tacagtatgt acaaagcaat tgtaacagat aatacaggat
    15901 gcaccaagta ccaagcagat ataatctcaa gtttgaggtc tctgctctgc ttagcttctc
    15961 ttctcgtccc ttaactaatt aaatcagcgt aaataggatg gacgatcttt tctaaatcgg
    16021 ctacttttat ccgaaggctt ctcccgattt caccgcttga cacaattact tcttcttttt
    16081 cttttaaaga actgtctgct aaaaaacaat aatcaggatg tgcgagtttt attccaatag
    16141 gggtgtttgc tccgtgggga tatcctgtat gcattaatac aaaatccatt ccaggaaaac
    16201 caactttacg atctttcgtc acttgtctcg tttttttgta atctaagtga tcgttcaaag
    16261 gaatcaatgc gatgatcacc ccagttttgt ttcctttcac tacgagagtt ttacatatca
    16321 aagctggatc aatccctctt ttttgtagtt cttctgtgaa agattcttct accacatgac
    16381 taaaatcaag tggttgatag ctgatctttt gttcttctaa ataacgttcg acttttgttt
    16441 gactcatcat tgattcactc cctttattct atcgtttatt ataaagcgag ggatgctgta
    16501 ttcaaaaat
//
LOCUS       DACCCM010000063        16362 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCCM010000063 DACCCM010000000
VERSION     DACCCM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.067"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(147..803)
                     /locus_tag="IVK78_002045"
     CDS             complement(147..803)
                     /locus_tag="IVK78_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6375497.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            996..1274
                     /locus_tag="IVK78_002046"
     CDS             996..1274
                     /locus_tag="IVK78_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375498.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1293..2501
                     /locus_tag="IVK78_002047"
     CDS             1293..2501
                     /locus_tag="IVK78_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375499.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2649..3014)
                     /locus_tag="IVK78_002048"
     CDS             complement(2649..3014)
                     /locus_tag="IVK78_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP6375500.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3161..3934)
                     /locus_tag="IVK78_002049"
     CDS             complement(3161..3934)
                     /locus_tag="IVK78_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6375501.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4108..4968)
                     /locus_tag="IVK78_002050"
     CDS             complement(4108..4968)
                     /locus_tag="IVK78_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP6375502.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4968..6386)
                     /locus_tag="IVK78_002051"
     CDS             complement(4968..6386)
                     /locus_tag="IVK78_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6375503.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6443..6892)
                     /locus_tag="IVK78_002052"
     CDS             complement(6443..6892)
                     /locus_tag="IVK78_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6375504.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7337..7600)
                     /locus_tag="IVK78_002053"
     CDS             complement(7337..7600)
                     /locus_tag="IVK78_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP6375505.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7699..8994)
                     /locus_tag="IVK78_002054"
     CDS             complement(7699..8994)
                     /locus_tag="IVK78_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP6375506.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8991..10262)
                     /locus_tag="IVK78_002055"
     CDS             complement(8991..10262)
                     /locus_tag="IVK78_002055"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6375507.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10427..11296)
                     /locus_tag="IVK78_002056"
     CDS             complement(10427..11296)
                     /locus_tag="IVK78_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP6375508.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11588..13198)
                     /locus_tag="IVK78_002057"
     CDS             complement(11588..13198)
                     /locus_tag="IVK78_002057"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP6375509.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14391..15731)
                     /gene="glnA"
                     /locus_tag="IVK78_002058"
     CDS             complement(14391..15731)
                     /gene="glnA"
                     /locus_tag="IVK78_002058"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP6375510.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15759..16151)
                     /locus_tag="IVK78_002059"
     CDS             complement(15759..16151)
                     /locus_tag="IVK78_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6375511.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4668 a         3471 c         2784 g         5439 t
ORIGIN      
        1 ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca
       61 aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact caggtcaaaa
      121 cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa caagtccata
      181 ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt cagttgtttt
      241 aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat ttcttcattc
      301 gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa atcatataat
      361 tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg ctgcattttt
      421 ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg tagctttggg
      481 tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc tgcatattct
      541 gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc ttttaaaaga
      601 gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc atttgaaata
      661 acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc catcccttcg
      721 aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt atccaaatat
      781 tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc taaaaagatc
      841 atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata ttaacatttg
      901 ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc gttatactgc
      961 agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg aacacaatca
     1021 ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct ttagtctttt
     1081 taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag aatccaacaa
     1141 acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt attcgccaac
     1201 ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa aaattaaaag
     1261 catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt aataaatatt
     1321 ttattggaat cacattgatc aattttattg tctacctgat ttattatctg ttgatggtca
     1381 tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca ggttttgctt
     1441 ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa cagttagaac
     1501 tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt tcaacattag
     1561 catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg aatggattcg
     1621 gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc cctgcatcta
     1681 aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc gctgttgggc
     1741 catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc tggttttcaa
     1801 ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa aatattccat
     1861 tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt attgaaaaga
     1921 aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca agtgttttat
     1981 catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca tttttcttcg
     2041 tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt ttcgatgtca
     2101 aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga ttatttttac
     2161 tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc ggcctaggtt
     2221 atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc gaacatcgaa
     2281 tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc gttggtcctt
     2341 atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg attgccggag
     2401 tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat agtcatgaag
     2461 cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga atagataaaa
     2521 aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata gtttatgatt
     2581 attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag aatagtttaa
     2641 ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag caaccacttg
     2701 tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt catccaaata
     2761 tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc tgaaccagca
     2821 tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc ccctagcaca
     2881 tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct aacaccgtat
     2941 aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac taccaacgcc
     3001 ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga ttatctaaca
     3061 agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat aaggtcctat
     3121 ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac agacattcga
     3181 tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt gtcaccagat
     3241 aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt ttttgctgtg
     3301 taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc attgcctctc
     3361 cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc attatcgcta
     3421 aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt gttgctcgtt
     3481 tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca ctcaacatag
     3541 cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct aggtttctga
     3601 ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct cgaatcaaag
     3661 aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg gtgatcttca
     3721 tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg cgaactctac
     3781 ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct tcgctataat
     3841 ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga taattcacaa
     3901 tctcctgctg tctattctcc acttcattca acataaaatc accccattta ctttatatag
     3961 taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag aagaaatttt
     4021 tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag actattgtca
     4081 taccctcttg accacacatt tttattttta gtataaacta gccttgccta ctgtgttcaa
     4141 aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg gataaattgc
     4201 attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat tgaaaaagac
     4261 gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg cttcgctgac
     4321 ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga tttgttggtt
     4381 caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat acaagccatg
     4441 cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa aaatcttcgc
     4501 ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac cattattccc
     4561 aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg caaccacttt
     4621 tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca tcacggaaga
     4681 atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat ccaagtggat
     4741 aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa actcatcact
     4801 taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta atctctctgc
     4861 ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg gtactgcaaa
     4921 ttgattctct ttagccactt tcaataactc tttcattgta tataacatta cgcttccacc
     4981 tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt cactgttttt
     5041 ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc caagacatac
     5101 ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc atgaggtgcc
     5161 attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc tgtcatcaaa
     5221 ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc tgtaatatac
     5281 gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt aaatttatgt
     5341 ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc agccatacaa
     5401 gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt cgaagtcgat
     5461 ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa tccgaacaat
     5521 agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa cacccatgtg
     5581 atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga taaaaagggg
     5641 acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt atttgccaga
     5701 aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa acctgaacca
     5761 cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt tccagcaatc
     5821 gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt atctcctccg
     5881 atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc agccgctgta
     5941 atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa tggaagcatg
     6001 taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt tgcagctggt
     6061 tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc tattactact
     6121 tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc ggcaaatcgg
     6181 tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt gatttcttgt
     6241 tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg atggtttaga
     6301 tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat tccagtcggg
     6361 caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca ctatcgatac
     6421 tcggacaaag actctactat tttcattctt gactaatctg tctgatgata tctgctggcg
     6481 tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg gagagtgtgc
     6541 tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca aaaatatatt
     6601 caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga aaaaaacaca
     6661 gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca tcttctattc
     6721 ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga acatttctta
     6781 ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct tttgaatcta
     6841 tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc ataaaaaacc
     6901 tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag catcgctagc
     6961 tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc gtgtataagt
     7021 cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa caaaaagcct
     7081 tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact tcgaaataac
     7141 ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa actatatact
     7201 agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa acgaagtttt
     7261 aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc taaacgttga
     7321 gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat tttttgttga
     7381 aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag aatggatgtg
     7441 agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc cattcaactg
     7501 tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt gaatccatga
     7561 atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg ctcctttgcc
     7621 ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca tcttaacatg
     7681 tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt taccaaagga
     7741 gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca aaaagcggat
     7801 cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt tccaaatttc
     7861 ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt ttttgatatc
     7921 gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt ctagatttcc
     7981 agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat cgactaaagc
     8041 ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc cgaaaaattt
     8101 cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc cgcttggtgg
     8161 aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga taacgacatc
     8221 tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa ctcgagtatg
     8281 gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc ttcgttctag
     8341 atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta cttccggata
     8401 gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg cttttggcgg
     8461 tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga tcattcgggt
     8521 agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa gaggtgttaa
     8581 ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa cgctttccac
     8641 gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc ctgccacttt
     8701 gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga tatcttctgc
     8761 acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa cgatatccaa
     8821 tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca cagctagaga
     8881 taattccttt ttattcatct tactgtaata aggaatttta aattctttgg cataactata
     8941 aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc tcattcagct
     9001 cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc gataatatga
     9061 tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc tgccaatagt
     9121 ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc tgtgccgtga
     9181 agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc agcgcccatt
     9241 cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat gatttctgaa
     9301 gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt ggcaaatcct
     9361 ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta aacttcgatt
     9421 tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat aaagctttcc
     9481 aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc tgctaatgct
     9541 agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc atgtaatgtt
     9601 tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc ccccatatta
     9661 ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc aattactgtc
     9721 tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac tgaaaccacg
     9781 tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata catggcacca
     9841 tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag gtcaatcggt
     9901 ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa tttaccaagc
     9961 agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg cggcatcgga
    10021 atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg gacagttgcg
    10081 cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg cacaccttcc
    10141 aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc actatttttt
    10201 gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac gatttttttc
    10261 atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt ttatcataga
    10321 taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga gagcgacttc
    10381 aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac gctttgttag
    10441 ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct ttacctggag
    10501 ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct tcgatatatt
    10561 tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact tttgagatac
    10621 ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca tgtaatacta
    10681 aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa gctaatcctt
    10741 tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag tagtcgatac
    10801 ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta ccgatgattc
    10861 cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct tttgcatgtg
    10921 ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga tgtgaaccat
    10981 cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg taatcaccat
    11041 ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa tcagtgatta
    11101 aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat gtttggataa
    11161 gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat tccaagttgt
    11221 ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa aattcagctc
    11281 ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat ttgttaagac
    11341 aattcttaac caacgaccct attttaccaa acatttcggt agttttctac taaaatcaat
    11401 gaaaatgaaa attttcaaga agttttttgc aaaaaataaa atatccaata gcgctttaat
    11461 cgaaaaaagt cttttgcttt ctatcttttt tcctacaaat aataacaaat cagaaaaaga
    11521 gaaaagtgac aagaaaattg cttatcccct tctcttgcta cttttctctt tctatcaagt
    11581 tcaaaaatta ttttgtttcg tgatctgcta atgatgctcc gacaaatgct ttgattaatt
    11641 tttgcgggcg atttggacga gaaatcaatt ctgggtggaa ttggcatccc acaaagaatt
    11701 ttttctctgg cagctctacg atttctacta aacggttatc tggtgagaca cctgaaaaga
    11761 ctagcccttg ttcttcaaac aattgacgat atttgttatt gaactcatag cggtgacggt
    11821 gacgttcttg cacaacatcg gctccatcat atgcttcagc cgtcttcgtt ccttttttga
    11881 ttttacatgg gtataatcca aggcgtaaag ttccacccaa attctcgatg ttttcctgat
    11941 cagccattaa atcaataata ttgtttgctg tatctggatt cgtttcagct gatgacgcat
    12001 cttctaatcc aacgacgtta cgtgcaaatt cgacacaagc catctgcata ccaagacaaa
    12061 tacctaaaaa cggaacgtca ttttcgcgag cataacgaat cgcttcgatt ttcccttcta
    12121 ctccgcgatc accaaatcca ccaggaacca agattccatc tgcgtctttc aaacgttctt
    12181 ctacattttc tctcgttaat tcttgtgctt tgatccaatc gatctcgatg tctgaattat
    12241 aggcgaaacc agaatgtttc aatgcttcaa ctactgagat atatgcatct ggtaactcga
    12301 catatttacc gacaagcgca atccgtactt ttttcttcaa gttcaagaca cgttcttcta
    12361 attctttcca ttctgtcatg tctgcttctg gtgtatctag ttttaaatgg tcgcaaacaa
    12421 tttgatccat tccttgtgct tgtaaaacta atgggatgga gtaaagtgta tctacatctg
    12481 gcgactcgat aactgcttct ggttcgacat cacaaaattg tgccaatttg tttttcacgc
    12541 tttgagaaac aggttgctct gtacgtacca ccaaaatatt cggttggata cctaaaccac
    12601 gtagctcttt tacactgtgc tgcgttggtt tagttttcat ttctccagca gctttcaagt
    12661 aaggaatcaa tgttgtatgg atatacataa cattatctgt accgacatct gctttcattt
    12721 gacgaagtgc ttcaaggaat ggcaaagatt ctatatcccc tactgttccc cctacttctg
    12781 taatgatcac atctgaatca gtcattttag ctgctcgcat gattttttct ttgatctcat
    12841 ttgtaatatg aggaataact tgaacagttg ctcccaagta ttcgccctta cgttctttac
    12901 gcaaaacttc agaatagatt tttcctgtcg tcacgttgga gtatttgttc aaattgatgt
    12961 caataaaacg ttcgtagtgg cccagatcca aatctgtttc agcaccatca tctgttacga
    13021 atacttctcc gtgctgatat gggctcatcg tccccggatc cacgttgatg tatggatcaa
    13081 atttttggat agttactttt aagccgcggt ttttcaataa gcgtcctaaa gaagctgcaa
    13141 caatcccttt tccgattgat gaaacaacac cgccggtcac aaaaatatat tttgtcataa
    13201 gttcaaaaac tcctttttaa tttatgtgca ggaaaagtct cagacaataa acaaagctcc
    13261 ctattcttca ataagaatag ggagccgaaa tttctgtaaa cttctgaccc tctaaaaggg
    13321 tgcccaaggt gtataataca agctgactaa gggaaagtca agtactcaaa tgaatactat
    13381 ccgcttacaa ttttttcttt ttcgatatcc tttgagaaat tatttcttaa gtcctttatg
    13441 agtgcaaaaa aaatttaacc cacataataa gcttgatata aacatagcta acgttaaacc
    13501 taagcaaatc agaaaatcgt tactgaaaaa ttttaagtga aataatagat aaaaaaataa
    13561 aacaaacatt atgatagata atatcccacc taacaaagaa ctactttttc ctacttgcag
    13621 caacagttta tatagcttat cgtagaaaca atctttttct ttcaccctta tttacctcct
    13681 ttacgtaaaa aacgtagtcc acagttctcg gatttttggc atatcttttc tattaaataa
    13741 tatcaaaaac aaaacaattc cactaatttt ttcaaagatg accttttttt caacaaaagt
    13801 gccttgtagg gattcattga aggtaacgaa tcacataagt aaaaaataag gagatgcgac
    13861 aaaagtcgtg cctccttatt tctaaataaa cggtgttctc ctgccaactc ctcggtcaca
    13921 agtcacaata tccatcaaac atgagaagct gtttttgaaa ttatttcttg tgattcggag
    13981 ttaaatggta ggctcacaac ctctggataa acggtattca aaagccagtt tctcggtatt
    14041 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct taatactcga
    14101 aactaaacgt ctttttcaaa acctcttggt aaacggtgtt caaaagcttg catctgcggt
    14161 aataagttca gccaattcaa aaacaagaaa agctgttttt gcttggctat tcttattact
    14221 tgttgcaggg cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc
    14281 ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgttagt ttctctaata
    14341 ctcaggtcaa aacgctgatt tcacagcctc taattcacgg tattcctgtt ttaataaagt
    14401 tctagatatt gttctctttc ccactcggaa acagtttgtc tgaatgccgc ccattcgaga
    14461 cgttttgctt caacaaaatt tgagaagata tgttgtccta atgcgtcaat cattacatta
    14521 tccttacgca attctttgat agcattatgg atagttgaag gtaagtcctg gatatgtgca
    14581 gcttctcttt cttcctcatc cattacataa atgttacggt caacagctgc tggcggtgtg
    14641 atctgattac ggataccgtc tagtcctgct tgcaacaata ctgccattgc caagtatggg
    14701 tttgcagatg gatcaactga ccgcaattcc aaacgtgtgg ataaaccgcg agattctggt
    14761 acacgtacta atggcgaacg attacggcca ctccatgcga cataaacagg cgcttcataa
    14821 cctggaacga gacgtttata agaattgact gttggattgc aaacggctgt gtaggcacga
    14881 gcatgtttca gcaaaccacc taagaaatga taagctgttt cacttagttc caattcaccg
    14941 tctttatcat agaaaacatt tccttcatca ttgaacaaag acatattgca atgcatacca
    15001 gaaccattga tcccaaataa cggtttcggc atgaaagtcg cgtgaagtcc atgcttgcgc
    15061 gcaatcgttt tgacaacgag cttgaaggtt tggatattat cacaagcatc taccacatct
    15121 gcatatttaa agtcaatttc atgctgtcct ggcgctactt catgatgtga agcttccact
    15181 tcaaagccta gactttctaa ttccaacacg atgtcacggc ggcaattttc acctaaatct
    15241 gttggtgcaa aatcaaaata accaccttta tcattcaagg tcgttgtgat cccaccgtct
    15301 tcgtctaatt taaataagaa gaattctggt tcaggtccta aattgaaaga ggtaaaacca
    15361 aggtcgcgca tatctttcaa tgctcgtttc aaattcccgc gaggatcgcc ggcaaatggt
    15421 gtcccatccg gattatagat atcacaaatt aaacgagcaa cttttccatg agcactttcc
    15481 cacgggaaga tcatccaagt ggagagatct ggatacaaat acatatcgct ttcttcaatt
    15541 cgaacaaatc cttcaatcga agaaccgtca aacatcattt tattttctaa tactttgtcg
    15601 atctggctca ctggaacttc tacatttttg atcgtaccta agatgtctgt gaacatcaat
    15661 cgtaaaaatc gtacgttttc ttcctcaatg attcgtttga tttcttcacc tgttatctgt
    15721 ttctttacca tggtatccct cttccttttt taatttattc agtcaaagaa ttgtctttgc
    15781 aatccttgcg actgataatg acctgttttg cttaacccac cttgcgccaa aatctcatca
    15841 cgaaaaattt gtcgaacgtc atgatctgtc aacggcttct tgttttttgc ttcttgtttt
    15901 ttcgcctgtg ccatttcgta tacacgtttg atcccagcca tattcaatcc ctctgaaaga
    15961 tagtctttaa tctcaagcaa catatcaata tcattcagcg aatacatacg tcggtttcct
    16021 tcgcttcgtt ccggatgaac taaatcttgt tcttcataat agcgaatttg ccgagcagac
    16081 aaatcagtca gcttcataac tgtaccaatt ggaaatacgg acatagatcg gcgcaattcc
    16141 ttttctccca tcagacgtcc cctttcgttt tataagttgg ctaaagtgta ccaattaaaa
    16201 aaaatattgt caactgcata tgttaggttt tctaacatga gatctctatt ttttataaaa
    16261 taacaaaaaa ataagaacac aaaaaaggta tctagttgat tttcgtacca gatacctttt
    16321 attttttatt atactgtact atcctttatt agtgttttta tt
//
LOCUS       DACCCM010000064        15471 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCCM010000064 DACCCM010000000
VERSION     DACCCM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.068"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1126)
                     /locus_tag="IVK78_002060"
     CDS             complement(<1..1126)
                     /locus_tag="IVK78_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6375512.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1209..1544)
                     /locus_tag="IVK78_002061"
     CDS             complement(1209..1544)
                     /locus_tag="IVK78_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6375513.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1554..1928)
                     /locus_tag="IVK78_002062"
     CDS             complement(1554..1928)
                     /locus_tag="IVK78_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6375514.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1941..2255)
                     /locus_tag="IVK78_002063"
     CDS             complement(1941..2255)
                     /locus_tag="IVK78_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6375515.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="IVK78_002064"
     CDS             complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="IVK78_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6375516.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(6009..7175)
                     /locus_tag="IVK78_002065"
     CDS             complement(6009..7175)
                     /locus_tag="IVK78_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6375517.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            7200..7526
                     /locus_tag="IVK78_002066"
     CDS             7200..7526
                     /locus_tag="IVK78_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375518.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDAGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            7679..8731
                     /locus_tag="IVK78_002067"
     CDS             7679..8731
                     /locus_tag="IVK78_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6375519.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            8807..9025
                     /locus_tag="IVK78_002068"
     CDS             8807..9025
                     /locus_tag="IVK78_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375520.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            9012..11585
                     /locus_tag="IVK78_002069"
     CDS             9012..11585
                     /locus_tag="IVK78_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375521.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKF
                     DSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSC
                     N"
     gene            11576..11773
                     /locus_tag="IVK78_002070"
     CDS             11576..11773
                     /locus_tag="IVK78_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375522.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            11777..12388
                     /gene="mobC"
                     /locus_tag="IVK78_002071"
     CDS             11777..12388
                     /gene="mobC"
                     /locus_tag="IVK78_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP6375523.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            12373..13764
                     /locus_tag="IVK78_002072"
     CDS             12373..13764
                     /locus_tag="IVK78_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6375524.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            13980..14294
                     /locus_tag="IVK78_002073"
     CDS             13980..14294
                     /locus_tag="IVK78_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375525.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            14287..14781
                     /locus_tag="IVK78_002074"
     CDS             14287..14781
                     /locus_tag="IVK78_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375526.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            14849..15163
                     /locus_tag="IVK78_002075"
     CDS             14849..15163
                     /locus_tag="IVK78_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375527.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            15262..>15471
                     /locus_tag="IVK78_002076"
     CDS             15262..>15471
                     /locus_tag="IVK78_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6375528.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         5203 a         2602 c         2783 g         4883 t
ORIGIN      
        1 gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc aaaatcagaa
       61 agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa atcaccgcac
      121 tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt aaaaagttgg
      181 gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt tctttcattg
      241 ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa atttcttcct
      301 tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca tttttgggat
      361 cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg aggaggaaat
      421 aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa tcataagccc
      481 atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg tctatcccaa
      541 ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct ttgagttcct
      601 tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt aaaagctgat
      661 cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct ttcattcgat
      721 aatagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc aaatctttga
      781 aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga atcatccgag
      841 ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga atataaagca
      901 atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct tttatagacc
      961 aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta aactcttgaa
     1021 aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga acaaagtgat
     1081 aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg atggtttcca
     1141 cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt tatgctagtc
     1201 atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa aaagccttca
     1261 atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg ccgacataag
     1321 ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca gtctagtaca
     1381 tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat aatcagaaac
     1441 attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc gccttttcca
     1501 tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt cacctatttc
     1561 tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa gactaaatca
     1621 tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt ggcaacggta
     1681 tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg taagggtaca
     1741 ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa attataggtt
     1801 cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac ttctcggcgt
     1861 aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat accttctgtt
     1921 aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa cacgtaatca
     1981 gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt cacggctttt
     2041 agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac cacctcaata
     2101 tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac cgtagtattc
     2161 cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt tccaaaggtt
     2221 tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt tcattgttag
     2281 ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac gttggttctt
     2341 aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat aatagcccgc
     2401 cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt tctccccggt
     2461 ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa cagttggtgt
     2521 ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta aggtttgaga
     2581 tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat cgtaattgat
     2641 ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt ttttcgcaag
     2701 cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag atttccaaat
     2761 ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag cttcttttga
     2821 gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca catcaccatg
     2881 agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa catggtgttc
     2941 cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg ctactggctt
     3001 atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga caatagtttt
     3061 atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg ttttctcttt
     3121 gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt catctttttc
     3181 ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc agtcttcttt
     3241 gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca gagaagttaa
     3301 ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt cctcaggcaa
     3361 atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca gcttctcgta
     3421 aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg taaagacata
     3481 ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt ctaatggcgt
     3541 aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc cataaggaag
     3601 attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg tggtcgcttc
     3661 atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa aggtcagatc
     3721 attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga aatcaaagcc
     3781 aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat ttttttcgct
     3841 atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat atccttcggg
     3901 cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg caacttggtt
     3961 cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct tcgctaactc
     4021 tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa acaaggtgta
     4081 ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt tgtcttcttt
     4141 cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac tggtaacaag
     4201 ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg tattcacgaa
     4261 cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg cattagcaat
     4321 ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa cgatttcacc
     4381 ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat tgtcattcca
     4441 aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt tttcccgtgc
     4501 gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca tgggggtggt
     4561 atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg taccatgagg
     4621 aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg caggtaaagt
     4681 ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt ctttatcgac
     4741 ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt aggtattagg
     4801 tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg cgacaggtgt
     4861 tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat tggcatttgg
     4921 ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat tttccacaac
     4981 ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg attgccctaa
     5041 tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt caatggcttg
     5101 atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc gttttattga
     5161 gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa tatgagtatc
     5221 tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat cggacatatc
     5281 tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga ctcctggttc
     5341 aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa cagtgacttc
     5401 tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg ctaaatctag
     5461 ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg taggagcaat
     5521 tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata atttcttttt
     5581 ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat aagatgctcg
     5641 cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat agaatggatt
     5701 tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc tttaaattag
     5761 aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat atatttacgg
     5821 gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga aaccaggtct
     5881 taagaaagca cgtaaagctt cacagttttc aaaacgttaa tcgttgttat accaacgatt
     5941 tcagacactt tctcattgcg agaaggtgtc tttttttcgt tctggtaatg agaaaggtaa
     6001 tgacaatatt ataaaccagt gtaagcaatg aatttttcta acgcatttcg ttgagctttt
     6061 tcagtaacat gagaataagt gttaagggta gtttcagcat ttttatgacc caatcgatct
     6121 tgtgtttctt tgatacttgc tccaccttct agtagaagag tagcgtgagt gtgccggaat
     6181 ccatgaatag taacgtgctt aaatttatat ttcttctcta aacgtttcaa tatattattt
     6241 gaatagtccg catgcaaagg ttgattaatg tcaccgtctg catttacata ggtaaagata
     6301 agttgatttt ttgtatttgt atttatgcca aactgcaaaa gctctcgagc ttgttctttt
     6361 ttccagattt ttaacatttc aatagtttgt gcatcaattg gtaatattcg ttcggaagat
     6421 atattttttg tatttttttg aatcctgaca gtttttcttc ttgcattctt ctcttcattt
     6481 cgaatagttg catagctctt tctaacatac attagctgtt cttcaaaata aatgtcagac
     6541 caatgaagag cagctagctc tcctttacgt ataccagtag taatcaaaag ctggaagatt
     6601 aaaatatgtt ttaaaggctc ttctttattg agaatatccc aaaaagcttt tagttcttct
     6661 aatgaaaagt aattttcatt ttctttcttt tcccgctttg gtagatttgt atttgccata
     6721 ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa atatttgaga gctgtaagac
     6781 ttaagctgtt tgatattctt gaattttaaa gcccattcat tgactgcatt ctgacagtga
     6841 acaggagtaa tcttagaaat ggacttattc ccgaattttg gtagtatatg caagcggaaa
     6901 aggtcttttg tacgttgata tgtcgtttct tgtacacgat tcctataaga aggcaaccat
     6961 tcttcataca ctgttataaa caagtcatta gaaggcttat gatagccggt atcttcaatc
     7021 tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt cagttttaaa cccttttttt
     7081 acatagtatt cttgctttcc attttcatct ttacgaacat acaagcggac actatatcgt
     7141 gcaccgcttt ttgttgtgta cttttgtatt gacataacta atctctccaa tctgataact
     7201 tggcgagttg gctgattgaa gaaatagaaa atgagaggag aaaaataatg gatgcaggaa
     7261 ttacagtaat gttagataaa caacaaacga atcaattgaa aaattatgtt tttgaaatga
     7321 caaaagaggc tattgatcaa gcaagaattg atgccggatt agaaagacca tttctaaaag
     7381 gaaaagagat ggctaaatat ttgaatgtat catatacaac ttttttgaaa tttaaaagaa
     7441 tgggattgcc agttatttta ttagaaaaaa tggagttgtt ttcaaaagaa gaatgcaaaa
     7501 aatggatatt gagtcatcaa atttaaaaaa cacctcgttt tttaaatttg atgaagtaga
     7561 aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg ggaacactca ataataaagt
     7621 tctgagaaaa ctttatttga atctattata gcaaataaaa tcagaagcag gtgtgacaat
     7681 gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc tattctaatt ctgatatata
     7741 tgcagaatta tactatcaat atcctatttt tttaaacgta aatgaaaaat ataagaaaat
     7801 gaaactaagt tctaagttag tttatatgtt actaaaagat agaaatagac tttcattgaa
     7861 aaataacttg tatgatgaga atcaaaaaat ttttttgata tttgaaatca aaaatctcgt
     7921 agaaatttcg ggaatgtcaa aaaatacaat cttatcagca ctagatgaac ttcaaaaagt
     7981 taaacttctt gtgaaaagaa aaaatggctt caataaaagt aaaatgcaaa tgttcccaaa
     8041 tttttattat ttgttaaagc ctgttctgtg caaagatgat gtttataaaa ttaataagat
     8101 aacccatcaa gaaaatagac aaaaaaactt gatagttcaa aatttgaact atggaacaaa
     8161 tcaatattct gttgatagtt caaaatttga actatgcgac acatcaatga aaacaagaga
     8221 caaaaacaat tttgctgata gttcaaaaaa tgaactagaa ttatataagt cttataaaga
     8281 tagtaaagat attaaagaat cacataatga tgatcttctt gaatcacttt caactaaaaa
     8341 taataaatta gatgaagaat tgatcgaaac atttattgaa gaaaatcttc ttaaacaaat
     8401 atggggagaa agtattgtga attgcttaaa actggctagc aatagtgatt atcgtcaatt
     8461 cgacaactgg tacaaaaaat tcaaatatgc gattagatct gctgaaaaag aacaaaaaat
     8521 tcaactgaaa attatctatg aaatttgtaa taacaagtat tttgtagatc atgttagaga
     8581 acagctatca atgactttac gtgacttgat acgaagagca aaaacagatc atcgtatcaa
     8641 acaaaaagac gattatatct ttgcttctct aaaaaataaa gcgttagaac taattgaatt
     8701 gcaaatcagc gaaggaatag ataagcaata agaatagaat tgagtcaaat gaaatggagg
     8761 ggtatctcca cttcatttga gaactttgaa tataggaggg gttattttga ttaaaaatcg
     8821 tttaatagat caagtttggt gtctagatca aaatacagat agtctcgaat taatcgagac
     8881 tatttgcttc aacactatag ttctcgattt aagagctgaa aatgataatt gtgtaactca
     8941 tgtcataatt aatcaaaaaa tggctcagaa gctctctgag agcctcagaa aatgggctaa
     9001 gggaggtaac gatgaaaata attgaaaaaa agattgccaa taaaaaaata aattggttgg
     9061 ttgttaattt tcttgttctg atagctatga ttatttttac aagtaatatt gctcatgcga
     9121 gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt
     9181 atagacctta cctaaaaaag tacaatggag atttccttag tcagttcggg atttggctcg
     9241 gatgggctgt ggtaaaaggc atgtttactg tgacagattc gattcaaaat atgattccag
     9301 atgtgctgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag
     9361 ccgtaaatac aattgtcatt ggattaatga tcctttcatt gatgtttgtt ggttacaaaa
     9421 tgattactgg aaaaggaaca gtggatttaa aatcggttgg aatgaacgta gtgatgtcta
     9481 tagcattaat acttttaatg ccaaccatga tttctagtgg tattcaattt gctaaaacat
     9541 tttataagga ctctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa
     9601 aacaaggagt aacagattta gcatatatta ataaaacaga tcagtactca agcattgata
     9661 aaacggagga tcgaaataat ttaacacgaa aaaattttca acagacggat ctcacacaag
     9721 tgttaacgga taaagtgatt gataaattgg aaaaagaaaa tccaattgcg gacaatcttc
     9781 gttatgaatt agttgaaaat tctaataatg aatttgttgc tacaaagttt tctgacaact
     9841 tcttatcaac attttctgat agcttgaaat caggctatta ccgataccaa gcaaatctat
     9901 gggggatttc aattgggttg acagcgttag caatcgccta tgtgttcagc gcgtttgtga
     9961 tcattacagc aatactagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcaa
    10021 cagatattga gacaggacag cgttcaaaag ttgttctatc agatattcta caatgttact
    10081 taactgttgg gtttcaagga tttgggttat caatgtttgc tatgtttatc aattttctga
    10141 actcaggaca aggtatctca acaaatattt ttataaaaac aattgcttat atctgtgccg
    10201 tttttgtttt aatcaaaggc agtggaacag tcatgagata ctttggtgtt gatattggac
    10261 tcaaagaagg ctatgggcaa cttgcttctg cttttggaat gggcgcaatg ctgtttagaa
    10321 aaggatcaaa tggatttaat cgtgctaaag gaagcggaga aggagaagat cgaaaaccag
    10381 aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa aactggaaga gctttgggat
    10441 atgcccatga acgtggatta tccggtttag cttcagatgg agctacaatg gctagtgaaa
    10501 gagcaacaaa accatttaag agtatgcgtg atatagctaa tgataccaag aataagttta
    10561 aagaaggttt agatgatggt acagtgtccg ctattaataa aaatagcaaa cctatgttag
    10621 ctaaaaataa agaagatgaa actggaaaat atgctgattc cgtgccaaca cgtctttcag
    10681 atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat tatgtctagt tctgaacgta
    10741 tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa tcctgcaagc gcaattcagc
    10801 aaaaagtcca acaagatatt gaagaacgta aaaatgctat gcacggtcaa gctaagtctg
    10861 ctgaagaatt aattaatcag aagcgtcatg aagcgaaata cacgcctgaa gcaatgaatc
    10921 gcgaggaaat aatgttaagg aaacgtgtag aagggcggac aggcgctaat attgacggaa
    10981 aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa ttcaaataca ggattagaaa
    11041 aattagtcaa agaaaatcca caaaattcta caagtagtca tggtggccga tcagtagatg
    11101 tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg acggaatgtt gatgtaagag
    11161 agaatttaca aagttcttca aatgctcaag gtactaagac aattgatgtt cgagaaaatc
    11221 tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt
    11281 ctacaagtag tcaaggattc aaaacagtag atgttcggga gaacattcaa aaagatagtg
    11341 gagaaatgaa agaaaaaaca caaaaaatca atatcgttga agacagaaag tcagcgaaaa
    11401 aatttgattc aaatcatgaa acagtgatta ttgatagtga aatccgtgaa aatgataagg
    11461 gatcaaaatc aagacgaaga ttcacttatg aagacaatga gttattcaga gatacactta
    11521 atgatcctaa tccacttttc gataatttat taagaaataa aaggggggga ttgtcatgca
    11581 attaattcta aagattagta cgcatgattt cttagaactt gacgaattat gtgaatcagt
    11641 aaagaacgca acaaatgaaa aaatcacggt cactaattat attgaactga tgattcaaga
    11701 agaattaaag atgaaaaatg aaatagagag caatttcaaa tgttgctctc tagaaaaaaa
    11761 tgtgagggga taatcaatgg atgataaaca aaaaatgtac gaaaaaaatc gcaaacatag
    11821 tgaggcctta aattttcgtg tcgataaaaa gacttatctt gaactgacat tgattgcaaa
    11881 acaaagggga atttctagag cagacgtttg ccgtgagcgg ttaaagggac tcaaatatga
    11941 accgccgttg ttagctgaag atgatagggt agttttaacc aaacagttaa atcaaattgg
    12001 taacaatttg aatcaagcgg taaaggcatt aaatatcatt gctaaatatt ttagagaaga
    12061 atctgaagat aaaaaagaaa agagcctaca ttttagtttt gatgctagcc gttacaacat
    12121 ttttaaagat gaacttgcga atagcatagc aaaaaagttg atgattagtt atccagatga
    12181 catttttttg gagtccaatc ggagtgcccc ctcttttttt aaattatcag aagtagaaaa
    12241 agaatatttc gaatattgga tagaaaagca aactagaccg ggtacgaaag aagagtattc
    12301 ccaaaaagtt gatcggaatt taattcactc aattcagatt attgcaagtt taaaagatga
    12361 ggtgaaacaa ctttgggagc tactataaca tcgaaaggac gcaagaaaaa tttatcctcg
    12421 ctattacaat atgcagctaa tccttcaaaa aaagaaaaat gtgttgcagt ctcaggaata
    12481 aattgtagtg atgatagtga ggaagcactg caagaaatga atttagtacg taccaagatg
    12541 aataaatcta aagatatagc acaagcgttt catgttattc attcgtttga taagagtact
    12601 tctaaagagt taagtattga taaaatgcac gaaatagctg tcgaatttgc agagaaagct
    12661 tttccaaatt cgcaaatcat tgttgcaagt cacaatgata aagatcattt tcatagtcat
    12721 cttgtcatta ataatattaa catggaaaca tgtaaaaggt tgcgtattga ccctaaagat
    12781 ttagaactaa tgcgaaaaat taacgatcaa gtcctcaatg aacatggtct tgaatcagtg
    12841 aataatgagt actatgaaaa cttagaaaag agaaccaatc tttattcaaa cgatccttct
    12901 aagatgaaat atggaaaaat taattatcaa gagtttgtgc aaaatgcgat cgaagcaacg
    12961 ttggaaaaat gcgaagtgaa ttcttttgaa gatttttcgg atcatttggc aaaagaatat
    13021 aacattgaag tttatcaatt ttcaaaaaca aataacaaac ttggctatgt tctttacaaa
    13081 caaggtgtaa atttctatga gcgtagaggt gagttaatta ctttaggaag agataaacaa
    13141 tctagaaaag aaaaaagtca gtatgtcgaa cgtactttta gtgcaagaaa gttagggaaa
    13201 agatattcat ttgaatctgt ttctgaacaa cttgaaaaga atttagaaga atcacatata
    13261 aaaaattttt ttaatatgga aagagagcaa gttatgtcgc taattagtgg agtaaattac
    13321 aaaactggtg agttaaatgt accgtttgtg gaattagatt ccatcgcaca aaagcaattt
    13381 agcgaccgtg aaggcgatga aaaattcatt aaatatgatg acttcttgcg agaggtagac
    13441 aagcattttg taaatacaat gtctttagaa gatgatcaag aatacacgat taaacttcat
    13501 acggatgtga atcctcaaac tttacgtagt gcgaaaagta aaacaatttc tattaaaaag
    13561 actgatcaga aagagttatt tctaccggca aagggttttg aaaattgttc tcatacacct
    13621 tctgctaaac agattcacta tcaaatcagt cgcataatga atgacgatgt aaaaatgtat
    13681 cgtgctaaaa aggattatga attcacacaa caaatgattg aaaatcagca aaaaaggcaa
    13741 caaaattttg aaatggaatt ctgaatgggc ttgttagtgg actttccagt tttttgccta
    13801 tgaagagttg cttggaaaga agcagttttt aggacatacg ccagctaccc atctacttgc
    13861 cagtcaatcc gccccgctca cgctaggcta ctttctggca agtcagatgg cgggtaaatc
    13921 tctgagggag caagctccct acagactccc tcccaaaaaa agaaaggtgg aagaaaaaaa
    13981 tgaactatcc atattttaaa gtctcggctt cagaagaaac taaagaaata ttcaacaatt
    14041 tttacaacca aaacaaagga atttttggtt caaaggcaaa tatgtttcgt gttatggttt
    14101 caaatttacc agtgttagct agtccatcaa ataataaatt taatgatcct gaaagcatta
    14161 aatttgaaca aaaaatttca gaactagaat caatgataag taatgaagta attgaaaagc
    14221 tggatgacat tgatcaaaag ttatcgtatt ctctaaaaaa caaatacaaa acggaggaga
    14281 aaaaagatgt ttaaagttta cttcccgttt cagtggctcg ttaaaggaat tcttttagtg
    14341 ctactaggat tgattctttt tggaagttat ctaacaatta aagccagaca ttttccagaa
    14401 gaaaagccaa taaaaattgt aacaaaagat ttaaaaatag gtgaaactta tcaggcagat
    14461 ggggttgaac taacatttaa aaatattgaa gtgaccgaaa aaagaaatga atttgcacaa
    14521 catgatcgtg tattacaagt gacctataca cttataaatg atacagaaaa tccgatacgt
    14581 ccacaacaat ggtcgattga aaataaggat ggtgaagaac tttatgagta tgatatgata
    14641 ttggataatc cagagtatat tgaaagtggc aaagaagaaa cagtaaatta ctattatgaa
    14701 ttaccgattg aaaggcagga ctaccaaatc gtagtacgta gagagaatgg ttacatagtt
    14761 gggttgccat ttactttata agttttattt tgttagtagt ttttaaggta atatcagttt
    14821 tgtgattttt gaaaatggaa ctgggagaat ggctgaggaa aataaagttg ttccgtttga
    14881 aacattagtg ataaataagg aagagtttga gcaacttttt cttgattttt ttattcaata
    14941 tgacatgatt tatgatttta gttcgtcact agatttattt gaattagaag cagaaataat
    15001 agaaaacagt cgagagaaga atggtaaaat cattatttca gtagaaaaat tcaatgaatt
    15061 attctcacaa acatttgaaa tagatccaga ctattatcaa ccacaagaag tttttcgaga
    15121 gtttagaaat aagattattg gttttagtca aactacaaaa tgaaagctta ataaagcaag
    15181 tgttgataga attattcctc aacgcttgct ttattttatg ggcacttcat aatagaggta
    15241 tagagaagag aggtattcat tttggatact attactccaa caaacttcaa agaaaatata
    15301 ggtaaagtgt tgcaagaagt agttcattca aattctgaaa tagaaattac tagggattct
    15361 aaaaatggat tgaacgatgg cgtcattatg atttctaaga gaaaatggga gttgttacaa
    15421 gaagaacttt atttacaacg aacgggtaca tgggcttatg tatctgattt a
//
LOCUS       DACCCM010000065        15230 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCCM010000065 DACCCM010000000
VERSION     DACCCM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.069"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            278..787
                     /locus_tag="IVK78_002077"
     CDS             278..787
                     /locus_tag="IVK78_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6375529.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVK78_002078"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVK78_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6375530.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IVK78_002079"
     CDS             2322..3302
                     /locus_tag="IVK78_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6375531.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IVK78_002080"
     CDS             3424..4842
                     /locus_tag="IVK78_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6375532.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IVK78_002081"
     CDS             4913..6382
                     /locus_tag="IVK78_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6375533.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IVK78_002082"
     CDS             6392..6859
                     /locus_tag="IVK78_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6375534.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IVK78_002083"
     CDS             6895..7767
                     /locus_tag="IVK78_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6375535.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IVK78_002084"
     CDS             7848..10382
                     /locus_tag="IVK78_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6375536.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IVK78_002085"
     CDS             10538..11311
                     /locus_tag="IVK78_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6375537.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IVK78_002086"
     CDS             11304..12095
                     /locus_tag="IVK78_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6375538.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IVK78_002087"
     CDS             12105..12596
                     /locus_tag="IVK78_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6375539.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IVK78_002088"
     CDS             12614..13024
                     /locus_tag="IVK78_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6375540.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IVK78_002089"
     CDS             13028..14428
                     /locus_tag="IVK78_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6375541.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IVK78_002090"
     CDS             14451..14825
                     /locus_tag="IVK78_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6375542.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DACCCM010000066        14995 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCCM010000066 DACCCM010000000
VERSION     DACCCM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.070"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVK78_002091"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVK78_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6375543.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVK78_002092"
     CDS             complement(322..891)
                     /locus_tag="IVK78_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP6375544.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVK78_002093"
     CDS             1077..1979
                     /locus_tag="IVK78_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6375545.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVK78_002094"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVK78_002094"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP6375546.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVK78_002095"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVK78_002095"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP6375547.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVK78_002096"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVK78_002096"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP6375548.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVK78_002097"
     CDS             8547..8732
                     /locus_tag="IVK78_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP6375549.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVK78_002098"
     CDS             complement(8794..9990)
                     /locus_tag="IVK78_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6375550.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVK78_002099"
     CDS             complement(10160..11596)
                     /locus_tag="IVK78_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6375551.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IVK78_002100"
     CDS             11889..12698
                     /locus_tag="IVK78_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6375552.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14881)
                     /locus_tag="IVK78_002101"
     CDS             complement(12884..14881)
                     /locus_tag="IVK78_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP6375553.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3178 c         2661 g         4779 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DACCCM010000067        14899 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCCM010000067 DACCCM010000000
VERSION     DACCCM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.071"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            279..803
                     /gene="infC"
                     /locus_tag="IVK78_002102"
     CDS             279..803
                     /gene="infC"
                     /locus_tag="IVK78_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP6375554.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            875..1075
                     /gene="rpmI"
                     /locus_tag="IVK78_002103"
     CDS             875..1075
                     /gene="rpmI"
                     /locus_tag="IVK78_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP6375555.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1128..1487
                     /gene="rplT"
                     /locus_tag="IVK78_002104"
     CDS             1128..1487
                     /gene="rplT"
                     /locus_tag="IVK78_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP6375556.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1739..3046
                     /locus_tag="IVK78_002105"
     CDS             1739..3046
                     /locus_tag="IVK78_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP6375557.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3123..5252
                     /locus_tag="IVK78_002106"
     CDS             3123..5252
                     /locus_tag="IVK78_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP6375558.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5227..5916
                     /locus_tag="IVK78_002107"
     CDS             5227..5916
                     /locus_tag="IVK78_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375559.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(6324..7010)
                     /locus_tag="IVK78_002108"
     CDS             complement(6324..7010)
                     /locus_tag="IVK78_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6375560.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7146..7340)
                     /locus_tag="IVK78_002109"
     CDS             complement(7146..7340)
                     /locus_tag="IVK78_002109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375561.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(7390..7830)
                     /locus_tag="IVK78_002110"
     CDS             complement(7390..7830)
                     /locus_tag="IVK78_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP6375562.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(7834..8679)
                     /locus_tag="IVK78_002111"
     CDS             complement(7834..8679)
                     /locus_tag="IVK78_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP6375563.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8828..10246
                     /gene="pepV"
                     /locus_tag="IVK78_002112"
     CDS             8828..10246
                     /gene="pepV"
                     /locus_tag="IVK78_002112"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP6375564.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(10303..11250)
                     /locus_tag="IVK78_002113"
     CDS             complement(10303..11250)
                     /locus_tag="IVK78_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6375565.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11473..11823)
                     /locus_tag="IVK78_002114"
     CDS             complement(11473..11823)
                     /locus_tag="IVK78_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6375566.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12023..13102
                     /gene="pepA"
                     /locus_tag="IVK78_002115"
     CDS             12023..13102
                     /gene="pepA"
                     /locus_tag="IVK78_002115"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP6375567.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13246..13566
                     /locus_tag="IVK78_002116"
     CDS             13246..13566
                     /locus_tag="IVK78_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6375568.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            13588..14052
                     /locus_tag="IVK78_002117"
     CDS             13588..14052
                     /locus_tag="IVK78_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6375569.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            14257..14862
                     /locus_tag="IVK78_002118"
     CDS             14257..14862
                     /locus_tag="IVK78_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP6375570.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
BASE COUNT         4739 a         2749 c         3114 g         4297 t
ORIGIN      
        1 gaatcactgc tagaaagtct gctaatctaa tcattttcag aaaaggaaga caaactgtat
       61 gaaaaacatt gacaaaaaga ttagtttttg ctatactatg atggttgaga aataaaagca
      121 gaagcacccg cttctcgcct tagttgataa tgtcactggg ctagatagtt ttcactggtt
      181 ctattaggtg aagtacgcgc gggttctgta agagaactcg tttttttatt gccgtttatt
      241 cggtaagctt ttctttctct cataatattt ggaggtgaat gaccatagca aaggatatga
      301 tggttaacga cggcattcgt gcacgagagt tacgattgat cggttcagat ggtgagcaat
      361 taggagttaa gacaaaagca gaagcattga atattgcaga acaagcaaac ttggatcttg
      421 tgttagttgc ccctggtgcg aaaccaccag ttgcgcgaat catggactac ggtaaatacc
      481 gtttcgaaca acaaaagaaa gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa
      541 aagaagttcg cttaagtcct acgatcgact tgaatgactt caatacaaaa cttcgtaatg
      601 cacgtaagtt cttggaaaag ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg
      661 ccattaccca taaagaaatt ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg
      721 atattgcaac agtggaacaa aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg
      781 caccgaaaaa cgacagtaag taatttgaat gactaactgg ctgttggaca gccgatagtc
      841 ggataacgaa agattttgag gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg
      901 atcagcaaaa cgtttcaaac gtactggtaa aggcggattg aaacgcttcc gcgcgtttac
      961 aagtcaccgt ttccacggca aaacaaaaaa acaacgccgt caattgcgta aagcaagcat
     1021 ggtttctagt ggcgacttca aacgtattcg ccaacaacta gcaaaaatga aataagaagt
     1081 acaaataaca gaatctgaag atttattttt attaggagga attatacatg gcacgtgtta
     1141 aaggtggaac agtaactcgt aaacgtcgta aaagagtgct aaaattagca aaaggttact
     1201 acggttcaaa acacacttta tttaaaacag caaaagaaca agtaatgaat tcatacaact
     1261 acgcatatcg cgatcgtcgt caaaagaaac gcgacttccg taaattgtgg attgctcgta
     1321 tcaacgcagc agctcgtatg aatggcttga gctactcaaa attaatgcac ggtttgaaag
     1381 ttgctgaaat cgacatcaac cgtaaaatgt tagctgactt ggctgttaac gatgcagcag
     1441 catttactgc attggctgac caagcaaaag aagctttggc taaataagct aaacaattat
     1501 aaagaggaaa gaagataacg aaaagttatg ttctttcctc tttttttatt tacaatcctc
     1561 catcaaatga aacaggacaa taacgtataa gtctaaaata gcataattaa ggttttttaa
     1621 ttgaaaactg aacatttatg gtcacccttt aatgaaagcg ctaactacat aagttagaat
     1681 gagtttgact gatatattag tttgcttatc agaaaaacaa aatggacgga ggaataacat
     1741 gagtgatatt attattggta ttttgttagt tatctcattc tttttcatgg tctggtactg
     1801 tgtaaaaggc tacaacttga tggtcggctt tgccatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccaatct tgatgtcatt aggtatttca gtaccggttg cactgttctc
     2221 atttatgggc tcgattatgg ctggaatctt tgctaatatc gtcaatttca aacaatatca
     2281 aacgatttat gctggtttca atccagcggc tgaaagctat acttataatg actatttcca
     2341 aattggtatg attgggatga ttgtaagctt agttgtggta ctaacggtcg ctaatatatc
     2401 aatgaacaaa aagaaacgct atgcaatggc agcaaacgta ccagctgaag gtggcgacgc
     2461 accaatgatc tcatggttag ccgtattatt accagtttta ggggtcgttc ttttagatct
     2521 tccaatcatc ttaggtttta ttctagcagg tatctgggca ttgctgttta ctggaaaatt
     2581 gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt
     2641 agatgttgca ccaatggttg gtttcttgat gaccttagcg atgttcaata attcagctgc
     2701 gtatgcttct ccttattttt cagctatttt tggagattgg ataccgcaat caccattagt
     2761 cttggcaatc gtatttgcta tattgacacc actaggattt ttccgcggtc caatgaatct
     2821 agtcggttca ggctcagcta ttttagctgt cgtattagct gttaatccaa cgatgtcgcc
     2881 agcctttttg ttcccgttat ttgcaatcac aacgattgct cctcagcatt tagatattac
     2941 acaatcttgg gttgcttggg gattaggtta tacaaaagta acttctcgag aatatatgaa
     3001 gaaatccatc ccaactggtt ggatcatcgg agcgatcttt gcctgatcac gttcttattg
     3061 tacgggaacg cctagtaaac aaaacgtcga taaaaacgat tagatgaatg gaggaaaaga
     3121 aaatgaaacg ttttgtgcga atgggaattg atgttggcgg tacacatacg aaagcagtcg
     3181 caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc agtgaaaaca acccacgatg
     3241 atgtccgtgg agtagccgca ggtgtcgttc agtcttttca aaattgtttg cgggaaaata
     3301 atatcagtcc agaagatgtt gtgtttgttg cacattctac cacacaagcg actaatgcac
     3361 tgattgaagg agacgtagca aaagtcggcg taatcgggat ggcaaaaggt ggattagaag
     3421 gttttttagc aaaacaacaa actagattga acgatatcga tttagggaat aagaaaaaaa
     3481 tagagattgt caatgcattc ttacctgtca aacacttaaa tgtagaccgt gtttcagaaa
     3541 cgatttcttc tttagaaaga gaaagagcgg aggtcttggt ttcttctatg gcatttggag
     3601 tagataatgg ggaaccagaa cgagttgttt atgaagcagc atcggtaaaa tcgatcccta
     3661 caacgatggc ttctgacatc acaaaattat atggattgac aagaagaact cggacagcag
     3721 caatcaatgc atctatttta ccaaaaatgc tagatacggc aacctctaca gaagattccg
     3781 tacgagaagc aggggtcaat gtttctttga tgattatgcg cggagacggc ggagtaatgg
     3841 aaatcaatga aatgaaaaaa cgacctgttt taaccatgct ttccggtcca gcagcatcag
     3901 tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt ttattttgaa gtaggcggaa
     3961 caacaaccaa tatcggggtc attaaaaatg gacgtccagc aatcgattat tcaattgtag
     4021 gtggccaccc aacatatatc tcttcattag atgttcgggt tctaggtgtg gcaggcggtt
     4081 cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg tccacgttct gcacatattg
     4141 ccggtctaga ttatgcagta tttacggaga cagaaaaaat caaaggtcct aaggttgaat
     4201 ttttctctcc taaagaagga gaccctgctg attacgtcaa ggtagtgatg gaagacggag
     4261 aagaagtgac tattacaaat acatgtgcag ccaatgtcct tggattagtg caagaagaac
     4321 atttttctta tggaaatgtc ccatctgcac gaaaagccat ccaagcatta gctgattatt
     4381 gtcatacgac ggttgaagac atcgcagaac agatcatgga aaaatcctat gctaagattg
     4441 aaccggtgat tttagaattg gcagacaagt atcacttgga gaaagatcag atttctcttg
     4501 taggtgtagg gggcggcgct gcctcattga ttacttactt tagtaataaa atgggtgtga
     4561 aatattcgat tcctgaaaac gccgaagtga tttcttctat tggtgtagca cttgcaatgg
     4621 tccgagatgt tgtagaacgt atcatccctt cgccaagtaa agaagatata cgatcgttga
     4681 aaaatgaagc gatgaataaa gcgattgaat caggcgctac accagaatcg atcgaagtcc
     4741 acgtagaaat cgatccacag acttcaaaag taacggctat cgctactggt tctacggaag
     4801 tgaaagcaac cgatctaacc aaggagatca caacagaaga agcactagag cttgctgcag
     4861 aggatatgcg attaaataaa aatgaagttt gcttattgga aaatacacct ttcttttatg
     4921 tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct tcggatcatc gatcaaaaag
     4981 gatttatcaa ggtccaacgt ggacatgctt catgtatgaa aacaactgca gccaattata
     5041 tgacagctgt cgaacaactt tgggaagata tggcagttta tcaaactgaa ttgattgccc
     5101 gaccagaatt ttatctctgc ttaggagcaa gagtctctga ttttacagcg acggatctgg
     5161 aacaactaca gcttttgatg gatctggaag tatctactat ggaaccggag gaagaagtga
     5221 tcgtcgttgc cggaaatatc aaacaaacat aagttagaag agttactaaa cgaattacaa
     5281 cagatccccg aagaaatttg gggattctac cagtttcggc gagatttatt ttggaaaaag
     5341 attcctttgt ccaagcagaa aattcttata caacaatcaa tcgattgtgg tatcgaaacg
     5401 gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag gagaaatttg tgaacaaatg
     5461 gctgttccaa tcgtttcctg tgaatcagag caaataaacg aacgaatcac ttttgcaact
     5521 tatgcagaag atgaagggat tcgattgatg acggagccgt tagaaaaatt aaaatgttca
     5581 ggactagctt cgatctctaa agaaacagcg caagctttga ttattggaca tgaactattt
     5641 caccacatcg aagcttctgt taagggaatt tatacgcaaa gcgaaaaaat tgtgctgtgg
     5701 cggttgcctt tttatacaca tcaatcaaac attcgtgcat tgagcgagat agcagcgatg
     5761 agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt atgtattaga ggcagtctta
     5821 ctttggccat ataatgaaat tcgtagccaa gggatcttag aagaaataaa aaaaatagaa
     5881 aaaagatgta cggagtatga tttagattac aaataaagag actgtgacaa aagtcgtgtc
     5941 acagtccttt ttttccgaat aaacgatgtt ctcctgtcaa ctcctcggtc acaagtcaaa
     6001 atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc ggagatgaac
     6061 ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
     6121 cagccaagtt aaaaacataa gaagctattt ttacttggct gttcttatta cttgatgcag
     6181 ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc agctgaccct cggtattagc
     6241 tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt tctttctcta atactcaggt
     6301 caaaacgctg attccaaaac ctcttatttg atgggtctgt cctgaatatc ttcaaaataa
     6361 tcggggtaat tatgaatcac gtattcttgc tcctcgataa tcatatgtaa atgcatcgtg
     6421 caaagaaatc cgacttcatc aaagttatga tcaatcattg attgaagcat tgtctgatgt
     6481 tcatctaata ctcgacccca gtctaattct gaaatcgtca aacgtaacca tctgaaacgg
     6541 ttcaagtgcg tactataact ttctagccaa tcccaaattt ctcgttttcc agcaatttca
     6601 taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag cttttttatt ttgagcgatt
     6661 actgcctgat cggtttgttc tagtattttt tctagtttag cttgatcttg cttggtcatt
     6721 ttcgcactgc attcttgcag gatcggtttt tccaactttt cccgcgtata acgggctaat
     6781 gaagcagatc ggatatcgat ttttgaaaca taagtgccgg actgtggaat ggtttttaca
     6841 agctcttgat tgcgcagttg gatcagtgct tctcgaatcg gtgtacgccc gatagctagt
     6901 tgttcttcta acactttatc ggagatttta cttccaggtg ctaactcgga ataaatgatt
     6961 tgtttacgta tagattcata agcttgctgt tgcaaattat tgataaccat ttaaccttcc
     7021 tttctatgca gatataatct attataacat aaatcgctta caaatcgtgt gggcataaaa
     7081 acacagtctg caaaaaaagc cctgataggc atgcctatta gagcctcgaa aagaaaaata
     7141 ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac gaccgccgat tcttttagca
     7201 aaagcttcgg attctttttt gtctttgaaa atgagcagat tgtctaagtt tttttcccga
     7261 gtgcgacagc cagttttatc gatgtaaccg tgcattactt taacgacata catggggatc
     7321 acctgctttt ttcttttcat tatacgaact actcgacaca atacaagaaa aatgcttaga
     7381 cggtctttat taagaattta catcatccaa agaagcgtac gcttggaata actgatctaa
     7441 ctccggattg actttgctca gcttcactaa tcgttgcgaa tcagaaagta aaaaacagtg
     7501 tttactactg ccagtagttt ttaatttttt gtcttcagtt cccacaattt cgtagctaaa
     7561 atccaaccgt gtccccgtat acttttggat agttggatgg atgctcactt tttctccgta
     7621 atggatcatt tctttatagc tgcaatttac ttccagaacg gggatgatga tcccagattc
     7681 ttcgattttt ttatagggaa accctagatg taccaataag gctacccgag cctcttcaaa
     7741 ccagcggata taattgctgt gatgaataat ccccatttga tctgtttcat aataaaaagg
     7801 ttctcgcaca taacctgtaa atttctccat gactcatgcc tcgttttcca ttgatttttt
     7861 acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt tgtgtcggca aaacaatata
     7921 ttgttctttt gccagctggt ctccaatgaa actatgaaga taaatagctg ctgcaatcgt
     7981 ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc atcccagcta aagtgtcacc
     8041 catgccgccc gttgccatag cagcagaacc taaaggattg gtgtagaagt gctggtgacc
     8101 ataaattttt gtctcatgac ttttcaaaac gagcacagca ccgatttcct gttgcttctt
     8161 tttgttttct ttatcccgtt gttcttgtat cggtagagaa ctcaaccgtt gccactccat
     8221 ttgatgaggt gtaagtacag tttgttcggg atagggtagc aaaagatgat tagcagcaag
     8281 taaacttaaa gcagagccat caatcaccag ccattgttct tttgattggt tttctagtac
     8341 ttgtttgaaa cgttccaaac tcttagaaga agtacccaat ccgggaccaa tcaagagtac
     8401 atctgcttgc tttattacgg aaagaaactg gtcagtatcc gtccaaacta caatcattgc
     8461 ttctggcaga tgactgtgaa gagcactccg atttttttca tcagttacta cagtcgtgag
     8521 acccgctccg ctgtgtacgg cagctaaagc actcataatg attgctccgc catattgttc
     8581 attgccgcca atcaaaacga tacgtccaaa tgttccttta tgtgaaattt ttggacgagg
     8641 tgtgatgatt cttttcacta gttgttcgtt tatcagcatc taaaaacctc cttttactca
     8701 ttatagcgta tatttttttt agagaaaaac agataagaaa aggaattgtc agattttttg
     8761 aactattttt ttctgtaatt tgtggtatca tgaacacgaa gacttaaata agtggaggat
     8821 taactttatg acaattgatt ggcaaaaaga agtagaagca cgtaaagaag acttgcttga
     8881 agacttgaaa aatcttttac gtgtcaacag cgagcgcgat gactcaaaag taacaccaga
     8941 tgcaccattt ggacctggac ctcgcgacgc attgaaacat atgttagctt atggcgaacg
     9001 tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg catattgatc ttggcgaagg
     9061 agacgaaacg ctagggatat ttggacacat ggatgtcgtt cctgcaggag atggctggga
     9121 cacagatcct tatgaaccag taatcaagga tggaaagatc tttgcacgcg gatcaagcga
     9181 tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag atcattaaag aacttgactt
     9241 gaaactatca aaaaaagttc gcttcgtagt cggcagcgat gaagaaagcg gctggggcga
     9301 tatggcttat tatttcgaac atgaagaaga accagatttt ggtttctcac cagatgctga
     9361 attcccaatc atcaatggtg aaaaaggaaa tgtttcttta gctttacgct tcaaaggcga
     9421 caatgcagga gattatgtct tgaaatcttt tgtttccgga cttcgcgaaa atatggtgcc
     9481 aggaacagca actgcagcac ttcaagtacc aagtgcagat gcagctatcg cgatggaaga
     9541 agcattttac caattcatcg aagcaaatcc agtaagcgga acgattgaag cggacaatac
     9601 gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt gcaagcccac aatctggtat
     9661 caatgcaggt tctttcttgg cattgttctt agataactat gaattcctag gatctgctaa
     9721 acaatttatc catgtagcag ctgcttatgt ccatgaagat ttttatggag aaaaactagg
     9781 agtcgcttat gaagatgaaa aaatgggcaa attgacaatg aatgctggac tatttgcttt
     9841 tgaagaaaat ggtacagagg aagcgaactt catcaatatg aatttccgtt ttccaaaagg
     9901 tgtaacagta gacggattgc aaagtgacat cgaacaaaca gtggggcaag aaggcgcaac
     9961 agttactcga ggtgctcgcg taatggaacc acattacgta ccaatggatg atccattggt
    10021 ggctacatta ttgcaagtct atgaagacca cactggagaa aaaggctacg aacaaatcat
    10081 cggtggcgga acttacggac gtttgttgaa acgtggcgtt gcttatggag cgatgttccc
    10141 aggatatact gatacaatgc atcaagcaaa cgaatttatg agtcttgatg acttgttcag
    10201 agcaacagca atctatgcag atgccatcta tcgtttggcg aaataattaa ttaaaaagag
    10261 gatcaagaat tgcaaattat ttttgcagtc ttgatccttt ttttactttc tactgtggta
    10321 ttcatgataa acatgatata gattcaattc atattctttc ttagcgttcg tgacgactgt
    10381 atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa gtcatcaatc cagtacttaa
    10441 aatcgcagaa gggatgcgtg taataaagct tgtcagcata tattcgcgaa tcacaggaat
    10501 ccccgtgacc aatccaaata aaaagaagaa gatggtccaa atcccaaaga aaagcaaagg
    10561 cttataatct ttaaacattt taataatcat catgatcact ttgaatccgt cagagaatgt
    10621 attcaatttt gattcgctgc cttcaggacg atcacgataa tcgattggga tttcaacgaa
    10681 cttgaatttt ttatctaaag catggatcgt caattccgtt tcgatttgaa aaccagaact
    10741 cataatagga aaacttttga caaagatacg attgaatcct cgatagccgg tcatgacatc
    10801 aagaattttt gttttataca ttctagtaat tgtatttcta acaaggttat ttccaaaatc
    10861 gtggaaaggt ctcttattct catcaaagta ggttccattt gataggcgat caccaatgac
    10921 catatctgct tcaccagaac gtaatttctc gagcaaacca tgtacagctt ctgcaggata
    10981 agtatcatcg ccatctacca ttaaataata gtcggcatcg atatcaaaga acatttggcg
    11041 gatcacattt ccttttcctt gacgaggttc tttttttacg attgctccac cttgaacggc
    11101 caattcgtat gttcgatcgg tcgaattatt atcatagaca taaatatctg cttctggtaa
    11161 ttctcgttta aagtctgcaa tgactgttga aattgttgct tcctcattgt aacaaggaat
    11221 cagaacagca attttttctt gttgttccat ttttcactct ccttaactat agaaaacaaa
    11281 agctaaatta aaaatacctc ataatcaaag gaaatgctag tttttcccgc taaatatcat
    11341 ttatttaaaa agaaaatact ggaatatatg tttggaaagc tctatctaaa aaaccaccca
    11401 ctacaaaaga aggacatgat acctctcttt tgaatagtgg gtgcgtattc gttctctcac
    11461 ttttttgacg tattaatctg ttgcaccttt ttttcttgaa atttctaata cggttcctgt
    11521 atgagcatct gctaaaaatt cataataaac agtttcgcca tcttctattc gagaaagacc
    11581 tccccgataa gcactcgacc gaacggcgaa tttcctaata ggttcctttt caaaagagat
    11641 ccaagaacct tcgattgcac cttcttttag aaaagatgac ttgatatttt caagtacttg
    11701 gtctgctgac aatacttttt tgcgatgata ccaaatcgtt gaagctaccc cgctcaataa
    11761 tcctagaccg attccaactg ctaacccgcc tttaaaatag cttagttcgc tttcttcata
    11821 catgtggaca tacctccttc ttctcacttg ttactccttg tgcttatttt accacactct
    11881 catattttct ttgaaataaa taaccgctac cagaaaaaat aagctataat aaatacgatc
    11941 ggtctgcaga aaaaatcata ttttatccgc agatcaatat aagtctgaga ggagtttagt
    12001 ctgaaattta gacgaatagc atatggaaga aaaaacattt caacgaatca aagagttaac
    12061 agaattacaa ggaacaagcg gttttgaaca agatgttcgc gcttatatgg aaagtcatat
    12121 ggaaccgctg gtagatgagt tacagttaga tggtttgggc ggcatttttg gtctgcgcca
    12181 tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca catatggatg aagtcggatt
    12241 catgctgaca caaatccaag aaaacgggct ttttaaagtc gtacctctag gcggctggaa
    12301 tccttacgtt gtttctgctc agcgttttac acttaaaacc tctactggta agaattatcc
    12361 atgtatttct tcttccgttc ccccacattt attgcgagga acaagcggac aaaaatcagt
    12421 agaagtaacc gatattttgt tcgatgctgg gtttgaatca agagaagagg caatgagtta
    12481 cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa acgatcaaaa cagccaacgg
    12541 aaagaatatt atcagtaaat catgggacaa ccgctatggc tgtacaatgg tattagaagc
    12601 tttagaagcg ttgcaaaatg aaacattagg tcatacattg attgcaggcg cgaatgtcca
    12661 ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat aaattcaagc cagatctgtt
    12721 ctttgcagta gactgctctg cagcagatga tacagtgacg aaaaatggca cattcggaca
    12781 tttaggagaa ggtacattga tgcgtatcca agatccaggt ttgatcatgc ttcctcgttt
    12841 gcgcgagtac ttgctggata tcgctgaaac aaacaatatc ccttatcaat actttgtttc
    12901 aaaaggcggt acagatgcag gtgctgctca tacacaaaac gaaggaatcc caagtacagt
    12961 gattggcgta gtgggacgtt atatccatac gcatcaaacg atgttcagta ttcgcgattt
    13021 tgaagctgca cgagaaatgt tgattcaaac actgaaaggt ttggataaat caacagtgaa
    13081 tacgatcgta tacggaaaat aatgcgatag tgaaactata aaatctggca aaagattgct
    13141 ttttgccaga ttttttttat aaaaagagga tcaatgtttg ttagttcgaa agaaaggtgt
    13201 ataatttaat tgaaaaatac ataaacagaa agtagaggac aaagaatgat tatcccaaat
    13261 agtttagaag aattagcagg ctacgtagaa actggaaaga gtgtattttt ctttactgcc
    13321 ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc ctgaaattga aaatgacttt
    13381 tctgattggc aatttattga agtagaccgt gacaagtaca tagatgtagc cgctgaatgg
    13441 aacatttttg gtatcccaag ttttgtcgtg atccaagacg gaaaagaatt aggtcgtcta
    13501 gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt tcctagaaag tgtaacaaaa
    13561 ggctgacatc tgttaggagg ggtcaagatg aatcaagaat ataagaatat tttagtaggt
    13621 atcgacggta gtgaacaagc acttgctgct ttcaaaaaag cggtagaagt ggctcgccgt
    13681 aataatggga aagtatatgt agcaaacgta atcgatcagc aattttataa ctttatgggc
    13741 tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg aaacagccaa ggaaatgatc
    13801 gaagaatgta aagaatatgg gaaatctgtt gattacacaa atatagaagg aattattgca
    13861 tatggttctg ttcgtgaatc catggcagtc aaattgccaa aaaaatatga gatcgatcta
    13921 atcatggttg ggcagtcagg tctaaatgca gtcgaacgct ttatgacagg aagtgtagcc
    13981 agctacgtga tacgtcattc accttgtgat gtcttgatcg tttctgacga agaagaacag
    14041 ccaagtaatt aagcaagtgg aacttgtacg acctttttaa tcaattaact aaaacagtaa
    14101 agggactgtg acaaaagtct tgtcacagtc ccttgttacg aataaatggt gttccagaag
    14161 aggcttcttc ggaaataaag catgaaaaaa atttcatgca gtgaatgcaa ttttttgtta
    14221 agataaatct cagcaaagga taatggagga aagaacatga tttttgctta taacaaagca
    14281 cacgtcggag acaccttgat ggttattata gccgacgata aaggaacaga aaacactgtt
    14341 gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag gtcaagttgt tgcttggaac
    14401 tttttccata tctccgatca tctaacaatc gaagggaacg gccaagtaag cttaacagat
    14461 gaggaattag cgaaattgaa tcgattgatc aaagaagcag gattcgaaga acagcttgaa
    14521 gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa catgcgtccc acatcctgat
    14581 tctgaccatt tatcgattac tgagacagaa gtagacaatg gtacagtatt gcaaatcgtt
    14641 tgcggtgcac ccaatatcga agctggacaa aaagttgtcg ttgcaaaacc aggagctatg
    14701 atgccagatg gaatgatgat ctggccagga actttgcgag gggtagatag ctatggaatg
    14761 atctgttccg ctcgtgaatt gcatttgcca aatgctccag caaaaaaagg aatcttagta
    14821 ttgcctgagg atgccgtagt cggagaagca tttccaaaat aaagaggttg tgacaagtct
    14881 ttgtcacaac ctctttttt
//
LOCUS       DACCCM010000068        14631 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCCM010000068 DACCCM010000000
VERSION     DACCCM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.072"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="IVK78_002119"
     CDS             complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="IVK78_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6375571.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1819..3042)
                     /locus_tag="IVK78_002120"
     CDS             complement(1819..3042)
                     /locus_tag="IVK78_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP6375572.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3073..3912)
                     /locus_tag="IVK78_002121"
     CDS             complement(3073..3912)
                     /locus_tag="IVK78_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP6375573.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3929..4609)
                     /locus_tag="IVK78_002122"
     CDS             complement(3929..4609)
                     /locus_tag="IVK78_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6375574.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGMTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4611..5684)
                     /locus_tag="IVK78_002123"
     CDS             complement(4611..5684)
                     /locus_tag="IVK78_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6375575.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6331..7002)
                     /locus_tag="IVK78_002124"
     CDS             complement(6331..7002)
                     /locus_tag="IVK78_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6375576.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="IVK78_002125"
     CDS             complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="IVK78_002125"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP6375577.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10426..10863
                     /gene="ndk"
                     /locus_tag="IVK78_002126"
     CDS             10426..10863
                     /gene="ndk"
                     /locus_tag="IVK78_002126"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP6375578.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10885..11751)
                     /locus_tag="IVK78_002127"
     CDS             complement(10885..11751)
                     /locus_tag="IVK78_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6375579.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11911..14004)
                     /locus_tag="IVK78_002128"
     CDS             complement(11911..14004)
                     /locus_tag="IVK78_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6375580.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4247 a         3137 c         2496 g         4751 t
ORIGIN      
        1 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
       61 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
      121 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
      181 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
      241 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
      301 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
      361 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
      421 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
      481 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
      541 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
      601 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
      661 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
      721 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
      781 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
      841 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
      901 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
      961 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     1021 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     1081 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     1141 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     1201 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     1261 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     1321 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     1381 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     1441 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     1501 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     1561 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
     1621 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
     1681 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
     1741 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
     1801 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
     1861 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
     1921 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
     1981 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
     2041 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
     2101 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
     2161 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
     2221 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
     2281 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
     2341 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
     2401 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
     2461 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
     2521 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
     2581 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
     2641 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
     2701 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
     2761 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
     2821 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
     2881 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
     2941 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
     3001 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
     3061 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
     3121 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
     3181 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
     3241 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
     3301 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
     3361 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
     3421 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
     3481 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
     3541 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
     3601 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
     3661 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
     3721 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
     3781 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
     3841 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
     3901 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
     3961 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
     4021 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
     4081 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
     4141 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
     4201 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
     4261 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
     4321 tcgtcattcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
     4381 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
     4441 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
     4501 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
     4561 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
     4621 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
     4681 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
     4741 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
     4801 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
     4861 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
     4921 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
     4981 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
     5041 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
     5101 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
     5161 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
     5221 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
     5281 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
     5341 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
     5401 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
     5461 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
     5521 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
     5581 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
     5641 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
     5701 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
     5761 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
     5821 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
     5881 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
     5941 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
     6001 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
     6061 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
     6121 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
     6181 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
     6241 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
     6301 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
     6361 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
     6421 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
     6481 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
     6541 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
     6601 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
     6661 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
     6721 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
     6781 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
     6841 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
     6901 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
     6961 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
     7021 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
     7081 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
     7141 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
     7201 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
     7261 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
     7321 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
     7381 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
     7441 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
     7501 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
     7561 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
     7621 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
     7681 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
     7741 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
     7801 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
     7861 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
     7921 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
     7981 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
     8041 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
     8101 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
     8161 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
     8221 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
     8281 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
     8341 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
     8401 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
     8461 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
     8521 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
     8581 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
     8641 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
     8701 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
     8761 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
     8821 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
     8881 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
     8941 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
     9001 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
     9061 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
     9121 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
     9181 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
     9241 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
     9301 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
     9361 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
     9421 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
     9481 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
     9541 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
     9601 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
     9661 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
     9721 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
     9781 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
     9841 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
     9901 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
     9961 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    10021 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    10081 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    10141 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    10201 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    10261 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    10321 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    10381 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    10441 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    10501 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    10561 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    10621 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    10681 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    10741 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    10801 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    10861 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    10921 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    10981 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    11041 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    11101 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    11161 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    11221 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    11281 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    11341 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    11401 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    11461 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    11521 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    11581 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    11641 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    11701 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    11761 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    11821 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    11881 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    11941 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    12001 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    12061 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    12121 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    12181 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    12241 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    12301 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    12361 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    12421 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    12481 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    12541 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    12601 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    12661 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    12721 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    12781 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    12841 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    12901 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    12961 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    13021 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    13081 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    13141 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    13201 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    13261 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    13321 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    13381 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    13441 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    13501 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    13561 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    13621 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    13681 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    13741 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    13801 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    13861 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    13921 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    13981 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    14041 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    14101 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    14161 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    14221 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    14281 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    14341 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    14401 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    14461 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    14521 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    14581 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat a
//
LOCUS       DACCCM010000069        14551 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCCM010000069 DACCCM010000000
VERSION     DACCCM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.073"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..153
                     /locus_tag="IVK78_002129"
     CDS             <1..153
                     /locus_tag="IVK78_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6375581.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
     gene            318..698
                     /locus_tag="IVK78_002130"
     CDS             318..698
                     /locus_tag="IVK78_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375582.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKRVYNYKLDNTLEAGDKIV"
     gene            829..1779
                     /locus_tag="IVK78_002131"
     CDS             829..1779
                     /locus_tag="IVK78_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6375583.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2297..2752
                     /locus_tag="IVK78_002132"
     CDS             2297..2752
                     /locus_tag="IVK78_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6375584.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            3063..3419
                     /locus_tag="IVK78_002133"
                     /pseudo
     CDS             3063..3419
                     /locus_tag="IVK78_002133"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3432..3842
                     /locus_tag="IVK78_002134"
                     /pseudo
     CDS             3432..3842
                     /locus_tag="IVK78_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3957..4121
                     /locus_tag="IVK78_002135"
     CDS             3957..4121
                     /locus_tag="IVK78_002135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375585.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4272..5251)
                     /locus_tag="IVK78_002136"
                     /pseudo
     CDS             complement(4272..5251)
                     /locus_tag="IVK78_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5256..6023)
                     /locus_tag="IVK78_002137"
     CDS             complement(5256..6023)
                     /locus_tag="IVK78_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6375586.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6513..6938
                     /gene="lacA"
                     /locus_tag="IVK78_002138"
     CDS             6513..6938
                     /gene="lacA"
                     /locus_tag="IVK78_002138"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP6375587.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6955..7470
                     /gene="lacB"
                     /locus_tag="IVK78_002139"
     CDS             6955..7470
                     /gene="lacB"
                     /locus_tag="IVK78_002139"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP6375588.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7481..8413
                     /gene="lacC"
                     /locus_tag="IVK78_002140"
     CDS             7481..8413
                     /gene="lacC"
                     /locus_tag="IVK78_002140"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP6375589.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8416..9396
                     /gene="lacD"
                     /locus_tag="IVK78_002141"
     CDS             8416..9396
                     /gene="lacD"
                     /locus_tag="IVK78_002141"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6375590.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9424..9741
                     /locus_tag="IVK78_002142"
     CDS             9424..9741
                     /locus_tag="IVK78_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6375591.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9741..11447
                     /locus_tag="IVK78_002143"
     CDS             9741..11447
                     /locus_tag="IVK78_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6375592.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11589..12995
                     /gene="lacG"
                     /locus_tag="IVK78_002144"
     CDS             11589..12995
                     /gene="lacG"
                     /locus_tag="IVK78_002144"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6375593.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            13018..13290
                     /locus_tag="IVK78_002145"
     CDS             13018..13290
                     /locus_tag="IVK78_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP6375594.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13311..14210
                     /locus_tag="IVK78_002146"
     CDS             13311..14210
                     /locus_tag="IVK78_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6375595.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4754 a         2401 c         2811 g         4585 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcggcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggtgcgt
      181 ttgattttca aacttcgcaa cagaaccgat gacactatcg ttcttcatca acgtaagtta
      241 gatttgttga aagagcagaa aaaaggattt ctacaaaaaa tgtttgtttg atattttaaa
      301 tagaaaagag agataaagtg aagtttttaa attttttgaa ctataaatat ttaaactggg
      361 tattatatgg tattacatta ttttttatta tttattactt ttttaccgat gatgatattt
      421 ttttaatttt agcaatactt tttcttgttt catctaattt tagtgataac ttaattgaag
      481 ttcttacaga accagaaatt cggtctatga cggatgaaga acaacagatt attaaagcta
      541 tgatttcgga aaataagaaa gacgtagtag ttatcaaaaa gattagagag tatacaaatt
      601 taggtgtttt acaagccaaa aaagtatatg aaaaaaaaaa aagggtctat aattacaaat
      661 tagataatac attagaagct ggagacaaga ttgtctagat aatgtgtgag ataaaatttt
      721 tggcaggtta tcatacataa gacaaaatgt ttttaaaact gataacgtat ttattccaca
      781 acaattacgc atttatttct gagaaaaata taaagcagga ggattacaat gctgaaaact
      841 tattccttaa caaaagatac attattagtt tcgtcagaat caaatatcaa aaattcttct
      901 gttgtttatg tttatggtga cgaacgagaa tatatcgaat cttctgaaca taaatatgac
      961 tttaaaatag gcaatatact aacgtttgat gatcgagttg catatgacac gatgatagat
     1021 aagaacgatg aaaaaagttt gctcgtaagt tttttgtttc ccgaggtaca tgaaggtggg
     1081 catctagata ccctgacgac atctgttgtt ttcttggtat ttaaaaataa gttgataatt
     1141 tttacaaaac aaaaattaaa attcattccc gaactcttat ccaatatggt tttacgtgat
     1201 gtcaatcatt tcgtggagga agtactttta aagatcatgc aaaaagattt ctatatgatg
     1261 cttaacgacc ttcgtggagt caaggaaaaa attgatgatt tagactcaga aatatcaacg
     1321 tccggttcac tcaaaccaac gtttggtgat ttattgactc ttcaaaaata catgatagct
     1381 ttgtcaacaa cttataaagc taaccataaa gcgcttgatt tcattaaaaa aaatttcaca
     1441 actattgacg atattgattt tagtactaaa aaaataattg atgatttata cgatacaagt
     1501 gatacaatgg atagaatcat tttaagttac agccaatatt tggacaaatt ggaaaatatg
     1561 ataaacaata tgatttcgta tcagctaaac atgattatga aaaccctaac cgagatatca
     1621 attgttttga ctattccagc aattattttt ggcttttggg gaatcaatgt ccatgtcccg
     1681 tttgaaaagt cttcttatgg tgttttagcc gtattgatta tctctgctat actaagtcta
     1741 atttgctggc ttttgatgcg acgcaaaaca tatttgtaaa gtatttgtga taccctaata
     1801 tgatttaaag cagaatttct ttgttgactt tttaaaaggc tgattccttt caataattta
     1861 atagcttcag cggctgctca gaagtcaata aattggacta tttttattca gttacttagg
     1921 caacatttga gatacgattg cgattataaa aattgatata ccaaccaatg gctgcttgga
     1981 agactgacat attagcctcg atatcatcga ataactaaat acagaaaaat acaataaata
     2041 ttatcaaact agggactata ctagaaaaca tcagtacatt ccttaactca gggtctataa
     2101 ttagaaaatc gcccctttga aaactgatta aaacagcccc cattatctag tgactagata
     2161 atgggggctg tttttttgtt aagtgatata ataaaccata aaagaagttt agaaatacgg
     2221 tcctggtcat caataaataa acaatttaat taattattta tttaattatt taatttcaga
     2281 aaggtataca tgaatcatgg tgcaacaaat tgtcctaccc atcaaagact ccagcgtctt
     2341 aaagatggta caagatacac ttctcgatag tttccgtgct ggtcgccgaa attacaccgt
     2401 ttttcaagtt ggtaaggcca cgctactacg tgtgagtgat gtcatgacat taaaaaaatc
     2461 agatgtctat aatccagacg gctctgtcaa aaatgcaacc tttattcatg ataaaaagac
     2521 cggtaaagca aacacgttat atttaaagcc tgttcagcaa gacttgttgc aatatcatga
     2581 ttggctggcc caagaaaata tcaattctga gtggttattt ccctcgacag cccattatga
     2641 tcttcatatc actgagaagc agtttcataa ggtgatggcg catgttggtg atctcttagg
     2701 aatcaacttg gaagacaaag agaaaaaatt gcgagtcaga aaaccgtttt gagatatgaa
     2761 cagcatttgc cttcacgcct tagcgatttt aaataatagc cttgaaagtg attatggaaa
     2821 agccctctac gctcaatgca aaattgacgt agaaactatt tttgatcgat tgaaaggtgt
     2881 atttggtatg tgaagaaccc atgtcagagg caagaacgtt gcacctactg acattggaat
     2941 gatgcttatg agcatgaatc taaccaaatt atcccttgag gctagaagaa aagcggaagc
     3001 ttttcaacac aaagcagcca aaaataaaaa acgcgacgaa gcaatcacat ttatgattat
     3061 tcatcgcgtt ttttatattt agagctagtt atttgctaga cccactattt acagatgaaa
     3121 cgtttatcac ctttggtttt gggcatttag tggaattagc agaaccaggt cattatgacg
     3181 aaaagtggca aaattggaaa cttgaatctt tgccgatttt tcctgatcga tacgattttg
     3241 aagttgcaaa agataaggga aagcagttta aaattgttgc agaacttctc aaaaaggcaa
     3301 atacaattat tgttgcaaca gatagcgaca gagaaggtga aaatatcgcc tggtcgatta
     3361 tccataatgc aaatgccttt tcaaaagata aaacatttaa aagactatgg atcaatagct
     3421 tagaaaaaga tgtgatccga agcggttttc aaaatttgca accaggaatg aattactatc
     3481 ccttttatca agaagcgcaa acacgccaaa ttgccgattg gttgatcggc atgaacgcaa
     3541 gccctttgta tacgttaaat ttacaacaga agggcgtaca aggtacattt tcactaggac
     3601 gtgttcaaac gcccacctta taccttattt ttcagcgcca ggaagccata gagaatttta
     3661 aaaaagaacc ttttttcgag gtggaagcta agtaaaacaa gggtcattta agggcgttct
     3721 aagccccaca cagcgtttta aaacccaaga ggagcttttt agcttttgtt tcttctaaac
     3781 aagctaaaat aggcaatcaa gaggggataa ttgctgatgt tcaaaccaaa gagaagaaaa
     3841 cgaatagtcc gagttcgttt tctttaagta gtttgcaatc aaaagtcaat cagctttata
     3901 aagcgacagc gagccaaact ttaaaagcta tgcaaggact gtatgaagca aaattattga
     3961 gttatccaag aacagataca ccatttatta cagagaacga atttgcttat ttaaaagcga
     4021 attttggcaa atatagcggt tttttaggac ttgatcttga aatgttgtta tcattgataa
     4081 tgatacgttc aaacaacagc acccaaattt tgacgaatta gtgaaactct atgaaaaaga
     4141 cgtagtaaaa catgcaacat cctattctaa tcagctggta aagcttaatt aggttatata
     4201 agcttgaaaa taaagggttt gtccaatatg gacaagcctt tttcttatgt tttgccacag
     4261 atttgccaca cttattgtgt aagagtatca aggataggta taatttgata ctttgttttt
     4321 ttagtgatgt gcaaataaat ttctgttaca cgactacctc tagaatgccc tacacggtct
     4381 tgaattgctt ctaggggtat tccttactct gctaagaaac taatatgcgt atgacggaat
     4441 atatgggtcg taatatgttt attaaaacta agtttattat caatatcaat tcctgctcgt
     4501 tttggattac cttttcgatt taattttcca ttttcaggtg agttgaggaa attgttaagg
     4561 acactataat gaagaggtgt tccattatag tcgggtttat gacgactttt ggaagtaaaa
     4621 atccatccat tatctttata atcaggattg aatctcatat gttggtgatt taactcaatt
     4681 tcctctttaa gaatttctat agccctttta gataattgga catttcggta actttgtaag
     4741 ttttttacat taccttcata gctattttct gcagctcctc cgtgagtgga ctgactaatg
     4801 atgatatttt gcttttcaaa atattgatag gcaccaatat tttggagttt cgactctgta
     4861 agtcaacagg gccattagtt agaataccta ttgggatgcg gtttttatgt agagccagca
     4921 gtagttgatg tatctcagga atcatcgtaa tctgatcgag ttcatattca tagtcagctt
     4981 ggaagtctag agcttctgtc ctagaaatac tatcgatgcc aaattcttcc atagtcttct
     5041 gacagcgaaa aattctaagc tgctcaatac tgatgagttt tttcgtgtat ttaggaaaag
     5101 caacatcaga ccaatatctg aaacgtttgt aaatgttatc tatttgttgg atatcaatcg
     5161 ttggaaaaca tttctcaacg ctacgtttaa aggggagttg atggtcataa agagtgtcat
     5221 caaggtcaaa aacaatattt ttataagtca taattctagt ccgcttttaa aatagtagtg
     5281 tactggctaa attcctctaa gtgttgaggg ctaatctggt tatctgtaat gatggtatcg
     5341 agttgatcta gattatagaa gttaaagaaa tcgtatcgat cgaatttagt actgtctact
     5401 aataagaatt tttctacagc attgtttagg gcaagttgtt gaatcacacc ttccttgtca
     5461 ctatatgtag caatagaatt atgggtaaca gcatttgcac taacaaaagc tttggcaaat
     5521 ctaattgctt ttaaatttgt cgaagccatt gaacctacaa aagctccagt tatttctcta
     5581 tattcaccgc caagaagcaa taaatcaatg gtttcgctat catttagaat caagaacaca
     5641 gggagactat ttgtaatgac acgaatttta taacctttac gacctttcaa ttctaatgcc
     5701 agttgttcaa gtgtagttcc aggtccaata aaaatagtat cgccatcttt gattaaagag
     5761 caggcttttt tagcaatgtc aattttttct tttgtattta gacttttctt ctcgatatgt
     5821 gtcttttcaa gtggcttttt agaggaaagc aattgtgcac caccatgagt acgtgttaaa
     5881 agtccatcag cttctagagc atcaaggtct ctacgggctg tcatatcgga aatatctagt
     5941 tcatctatta tttcttttat ggttatggtc ccatcaatct ttaacatatc taaaattttt
     6001 tctaatcgtc gttttttgtt catatgaaga cccttctttc ataaagtaat ttttttccaa
     6061 agataattct cttttaattg tatcataaaa gacaatattt tcaaggtaaa acaaacaatt
     6121 tcaaacaaaa acaaatgtta gatgatgaaa taagaacaga ggattgacgt atattagctt
     6181 aggtcagatt ttgtataaga cgaaaataaa gtaggacctc ttaatcagta agttatagaa
     6241 agtaaaagac ttttgtaata cttgaataga tatttcacgt ccattttgtg atggattaaa
     6301 tgaacaaaaa tgaacaataa tttaacggtg ttatctattt tttaaaaaaa caaataaaaa
     6361 aaacaaaaaa taaacaaaaa aagtgttgct ttttgtttga atgcttgata ttatataaac
     6421 aaagaaatga tgaaaacgtt ttttcgaaca ttttgcaaaa tgttttctac ttctacgtag
     6481 catttctttc taaaatttag gaggtggtca aaatggctat tgttgttggt gcagatctca
     6541 aaggtacgag attaaaagat gttgtgaaaa attttcttgt agaagaaggt tttgaggtaa
     6601 ttgatgtgac taaggacgga caagattttg ttgatgttac cttggcagtt gcctctgaag
     6661 taaataaaga tgagcaaaac ttaggtattg taattgatgc ttatggagct ggtccattca
     6721 tggtagcaac taaaattaaa ggtatggttg cagcagaagt ttcagatgaa cgctcagctt
     6781 atatgacacg tggacataac aattcacgaa tgattacggt tggcgctgaa atcgtcggtg
     6841 atgagcttgc taaaaacatc gctaaaggtt tcgtcaatgg taaatatgat ggcggacgtc
     6901 atcaaatccg agtagatatg cttaacaaaa tgtgttaaga aaaggagtta aggaatgaga
     6961 atcgcaattg gatgtgacca cattgtcaca gatgaaaaaa tggcagtatc agaattcttg
     7021 aaatcaaaag gacatgaggt ccttgatttc gggacatatg accacgcacg tactcactac
     7081 cctatctatg gtaaaaaagt cggtgaagca gtagtaagtg gtcaagcaga cttgggagta
     7141 tgtatctgtg gtacaggtgt cggcattaac aatgcagtca ataaagttcc aggtgttcgc
     7201 tcagcgttgg ttcgtgatat gacatcagct atatatgcaa aagaagagtt aaatgcgaat
     7261 gttatcggtt tcggtggaaa gattactggt ggtcttctta tgaatgacat cattgaagct
     7321 ttcattgaag ctgagtacaa accaacagaa gaaaataaaa aattgattgc aaaaatcgaa
     7381 cacgttgaaa cacataatgc acatcaagca gatgaagatt tctttacaga attccttgaa
     7441 agatgggacc gtggtgacta ccacgattaa ggtataacaa atgattctga cagtcacact
     7501 aaatccttca gtagacatct cttatcctct agaaacattg aaaattgata cagtgaatag
     7561 ggtgaaagat gttagtaaga cagctggtgg aaaaggctta aatgttacta gagtcttgta
     7621 tgaatctggt gataaagtta ctgctacagg tttcttaggt ggtaaaattg gtgaatttat
     7681 tgaaagcgaa ttagaacaat ctcctgttag tccagctttt tataaaatct caggaaacac
     7741 tagaaactgt attgctatct tacacgaggg taaccaaaca gaaattttgg aacaaggacc
     7801 aacaatttct catgaagaag cagaaggctt tcttgaccac tatagcaatc ttattaaaca
     7861 atcagaagtt gtgacaattt caggaagttt accttcagga cttcccaacg attattatga
     7921 aaaacttatc caacttgctt cggatgaagg agtagcagtc gttttggatt gttcaggtgc
     7981 acctcttgaa acagttctaa aatcgtcggc taaacctact gccatcaaac ctaataatga
     8041 ggaactttca cagttgcttg gaaaagaagt aacaaaagat attgaagaac tcaaagacgt
     8101 tcttaaagag tcacttttta gtggtatcga atggatagtg gtctcattag gtcgaaacgg
     8161 tgcctttgca aaacatggtg atgttttcta taaggtagat attcctgata tcccagttgt
     8221 taatccagtc ggatcagggg actcaacggt agctggtatt gcatcagctt taaatagtaa
     8281 aaaaagtgac gctgacttat taaaacatgc gatgacattg ggtatgttaa atgctcaaga
     8341 aacaatgaca gggcatgtga atatgactaa ttacgaaaca ctaaactcac aaattggagt
     8401 aaaagaggta taaaaatggt acttacagaa cagaaacgca aatctttgga aaaaatttca
     8461 gataaaaacg gtgttatctc agctttggca tttgaccaac gtggtgcttt gaaacgtttg
     8521 atggcacagt atcaagatac agaaccaact gtagctcaaa tggaagaact aaaggttttg
     8581 gttgctgatg aacttacaaa atacgcttct tcaatgttgc ttgaccctga atatggactt
     8641 ccagcaacaa aagcattgga taaagaagca ggtcttcttc ttgcctatga aaaaacaggt
     8701 tatgatacat caagtacaaa acgtttgcct gactgcttgg atgtttggtc tgcaaaacgt
     8761 atcaaagagc aaggcgctga tgccgttaag ttcttgcttt actatgatgt agacagctca
     8821 gatgaactta accaacaaaa acaagcttat attgaacgtg ttggttctga atgtgtcgct
     8881 gaagatattc ctttcttctt ggaaatcctt gcttatgatg aagaaatctc agatgcaggt
     8941 tctgttgaat atgcaaaagt gaaacctcgc aaagtcattg aagcgatgaa agttttctct
     9001 gatccacgct ttaacattga tgttcttaaa gtagaagtac cagttaacgt taaatatgtc
     9061 gaaggttttg ctgatggtga agtagtttac agtaaagctg aagctgctga cttctttaaa
     9121 gcacaagaag aagcgactaa ccttccatac atctacctaa gtgcaggtgt atctgctaaa
     9181 ttgttccaag aaacattgca atttgctcac gactcaggtg ccaagtttaa tggtgtgctt
     9241 tgtggacgtg ctacttgggc aggatctgta gaaccttaca tcaaagaagg tgaaaaagca
     9301 gcacgtgaat ggttgcgtac tactggattc gaaaatattg atgaacttaa caaagttctt
     9361 gttaaaacag ctagtccatg gactgataaa gtatagtaaa aacataaaac ggaggatatt
     9421 gttatgaaca gagaagagat gactctctta gggtttgaaa ttgttgctta tgctggagat
     9481 gctcgctcta aacttttaga agcgcttaaa gcggctgaaa atggtgattt cgctaaggca
     9541 gatagtcttg tagtagaagc aggaagctgt attgcagagg ctcacaattc tcagacagct
     9601 atgttggctc gagaagcttc tggggaggaa cttccataca gcgttaccat gatgcatggt
     9661 caggaccact taatgactac tgtcttatta aaagatgtga ttcatcacct catcgaactt
     9721 tataaaagag gagcaaaata atgcataaac tcattgaact tattgagaaa gggaaaccgt
     9781 tctttgagaa aatctctcga aacatctatc ttcgtgctat tcgtgatgga tttatcgctg
     9841 gtatgccagt catccttttc tcatctatct ttatccttat tgcctatgtc ccaaatgctt
     9901 ggggattcca ctggtcaaaa gacattgaga ccttcttgat gactccttat agctattcga
     9961 tgggtatcct agcattcttt gttggtggta ctaccgctaa agctttgacg gactcaatga
    10021 accgtgacct acctgcgacc aatcagatta acttcttatc tacgatgcta gcgtctatgg
    10081 tagggttcct tttgatggca gctgagcctg caaaagaagg aggcttcttg acagccttca
    10141 cgggaacaaa aggtcttttg acagcattta ttgcagcctt tgtgacagtt aatgtttaca
    10201 aggtctgtgt gaaaaataat gttactattc gtatgcctga agaggttcca ccaaatatct
    10261 ctcaagtatt taaagacttg attccattca ctgtagcggt tgttctcctt tatggatttg
    10321 aactcattgt taagggaact cttggtgtaa ccgttgcaga atcaatcggt acccttcttg
    10381 ctcctctctt ctcagctgca gatggttacc ttggaattac acttatcttt ggtgcttatg
    10441 ccttcttctg gtttgttggt atccacggtc cttcaattgt cgaaccagca atcgctgcta
    10501 tcacttatgc caatatcgat gccaacttgc atcttatcca agctggacaa catgcagaca
    10561 aagttatcac ttctggtact caaatgttta ttgttaccat gggtggaaca ggagctacat
    10621 tgattgttcc attcttgttc atgtggattt gtaaatcaga acgtaaccgt gccatcggac
    10681 gtgcctcagt tgttccaaca ttctttgggg ttaatgagcc aatcttattt ggtgcaccaa
    10741 tcgtattgaa tccgattttc tttgtaccgt tcattttcgc tccgattgtc aacgtttgga
    10801 tctttaaatt ctttgttgac acattgaaca tgaactcatt ctctgccaac cttccatggg
    10861 ttactcctgg tccgttggga attgtactcg gtactaactt ccaagttcta tcatttatct
    10921 tagccggact cttggtagtt gttgatacta tcatttatta cccatttgtt aaggtatacg
    10981 atgaacaaat tcttgaagaa gaacgttctg gtaaaacaaa cgatgctctt aaagaaaaag
    11041 tagctgcaaa cttcaatact gctaaagcag atgccgttct tggaaaagca ggcgttgcta
    11101 aagaagatgt agcagcaaac aataatatca caaaagaaac taatgtcctt gttctttgtg
    11161 caggtggagg tacaagtggc ttgcttgcca atgccttgaa caaagcagca gcagaataca
    11221 atgttccagt caaagcggca gcgggtagtt acggtgctca ccgtgaaatg ttgccagagt
    11281 ttgacttggt tatcttggct ccacaggtcg cttcaaactt cgatgatatg aaggctgaaa
    11341 cggataaatt gggcattaaa cttgcaaaaa ctgaaggagc tcaatatatc aaattgacac
    11401 gtgatggaca aggtgctctt gcatttgttc aacaacagtt tgattaataa atttgtaaaa
    11461 gttatttgag acgagattat tctttcaatt ccttataata ttgttaacca tatcattgca
    11521 tttcatttcc ttggtttccg gaactcagaa tatcgtctca aatgcttttt ttgaaaggat
    11581 ttatagttat gactaaaaca cttcctaaag attttatttt tggtggtgct acagctgctt
    11641 atcaagcaga aggtgcgact cacacggatg gtaaaggccc agttgcatgg gataagtatc
    11701 ttgaagacaa ttattggtac acagcagagc ctgcaagtga tttttaccat aaatatcctg
    11761 ttgatttaga attagctgaa aaatatggcg ttaatggtat tcgtatttca attgcctggt
    11821 ctcgtatttt cccaactggc tatggcgaag taaatgaaaa aggtgttgaa ttctaccata
    11881 atctttttgc cgagtgccac aaacgtcatg ttgagccatt cgtgactttg caccactttg
    11941 acacaccaga agttcttcac tcagatggag atttcttaaa ccgcgaaaat atagaacact
    12001 ttgtagatta tgccgctttc tgttttgaag agttcccaga agtaaactac tggacaactt
    12061 tcaatgaaat tggcccaatt ggagatggtc aatacttggt tggtaaattc cctccaggca
    12121 ttcaatatga tcttgccaaa gtcttccaat cacaccacaa tatgatggtt tctcatgcac
    12181 gtgctgtgaa attgtataaa gataaaggtt ataaaggcga aattggtgta gtacatgcgt
    12241 tgccaaccaa atacccttat gatccagaaa atccagcaga tgttcgtgcc gctgagttag
    12301 aagatatcat ccataacaaa tttatcttgg atgcaaccta ccttggacac tattcagata
    12361 aaacgatgga aggggtcaac cacatcctag ctgaaaatgg tggagaactt gatcttcgtg
    12421 atgaagactt ccaagcactt gaagcagcta aagatttgaa cgatttcctt ggtatcaact
    12481 actacatgag tgattggatg caagcctttg atggtgagac tgaaatcatt cacaatggta
    12541 agggtgaaaa aggaagttct aagtaccaga ttaaaggtgt tggtcgccga atcgcaccag
    12601 attatgtgcc tagaacggat tgggactgga ttatttatcc tgaaggcttg tatgaccaaa
    12661 tcatgcgagt gaaaaatgat tatccaaact acaagaagat ttacatcact gaaaacggtc
    12721 ttggatacaa agatgagttt gtagataata ccgtttatga tgatggtcgt attgattacg
    12781 tgaagaaaca cttggaagtt ttatcagacg caattgcgga tggtgcaaat gttaaaggtt
    12841 acttcatctg gtcacttatg gatgtcttct catggtcaaa cggatacgaa aaacgttatg
    12901 gactattcta tgtagacttt gacacgcaag aacgctatcc taagaaatca gctcattggt
    12961 ataagaaagt agcagaaact caagtgatag agtaatatta cttgaaaatg taaaaaattg
    13021 ttatccgata ctttagaaaa agtacaacac ttatttagac caggctttga aaaggtatat
    13081 attgttagtt tcgaagattg tcctatgatt cctgagcttg aagctacccc gttgctaaaa
    13141 tgtggtaaat ggtatgtttc aactggaaaa gagtggattt gccattctga tttagagtta
    13201 tcagcatttg aatgggaatt tttacaatca ctcgatgtag aaatacgtga gactattcac
    13261 ttcgaggtaa attatttgcc tttccaataa taaaaagaaa ggaattagtt gtgactatcg
    13321 aattaaaaaa cgagtatctt actgttcagt ttaaaacact tggtggtcaa ctgacttcga
    13381 ttaaagacaa ggatgggata gagtatctct ggcaagctga tccaaattat tggaatggac
    13441 aggcgcctat tctatttcct atctgtggta gtttacgaaa tgattgggct atttatagac
    13501 ctcaagaaag accctttttt acaggtctta ttcgtagaca tggttttgtt cgaaaagaag
    13561 aatttattct ggaagaagtc aatgataata gcgtgacctt tagtattaaa ccaaatgctg
    13621 agatgcttga taattaccta tatcagtttg aactgagagt tgtttatacc ttgaatggta
    13681 aatcaattag aaccgaattt caggtaacga acttggaaat ggaaaaaaca atgccttact
    13741 ttattggagc ccatccagca ttcaatagtc ctttagtaga aggtgaaaac tatgaggatt
    13801 attatattga atttagtgag gttgagtctt gctcgatacc aaagagtttt cctgagacag
    13861 gattattaga tttgcaagat cggacacctt ttttggaaaa tcaaaaaatt ctcgatttgg
    13921 actactcact tttttcccat gatgccatta cacttgacca gctcaagtct cgtagcgtaa
    13981 cgcttcggtc aaggaagtct ggtaaaggtc ttcgagtaga ttttgatgat tttccgaact
    14041 tgattctgtg gtctacaact aataaaagtc cattcattgc tttggaacct tggagtggtc
    14101 tctccacttc acttgaagaa ggtaatttct tagaagataa aagacaggta acgaaaattt
    14161 cacctcaaga aacttctaga aaaatctatg atattactat ccttaattaa aatttgttat
    14221 tatttgagtc ttcaataaga tttgaatact cttaccttgt gatgatagat tggtcttctc
    14281 cgtgccagtc ttacatcgca aggtttttaa gttttttgtg ggtctatgtc aactgtagtg
    14341 ggtgacgaaa agctaatatc taaagacagt ctggtagtaa aaaataagat tatttactac
    14401 ttcttgattt aaaataatag tggagccatt aatttgtggc aaatttaatt taatattttg
    14461 cctcaaatta gccacacttt ttatagaact aagaggaaaa atgaaatttt cttcctatta
    14521 atatagttgt ttgaaatctt aaatatagcg t
//
LOCUS       DACCCM010000070        14548 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCCM010000070 DACCCM010000000
VERSION     DACCCM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.074"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..816
                     /locus_tag="IVK78_002147"
     CDS             4..816
                     /locus_tag="IVK78_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP6375596.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IVK78_002148"
     CDS             813..1613
                     /locus_tag="IVK78_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP6375597.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IVK78_002149"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IVK78_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP6375598.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IVK78_002150"
     CDS             3110..4819
                     /locus_tag="IVK78_002150"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP6375599.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IVK78_002151"
     CDS             5031..9389
                     /locus_tag="IVK78_002151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP6375600.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IVK78_002152"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IVK78_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP6375601.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IVK78_002153"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IVK78_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP6375602.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IVK78_002154"
     CDS             11227..11520
                     /locus_tag="IVK78_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP6375603.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IVK78_002155"
     CDS             11517..11828
                     /locus_tag="IVK78_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP6375604.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IVK78_002156"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IVK78_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP6375605.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IVK78_002157"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IVK78_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP6375606.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3768 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaa
//
LOCUS       DACCCM010000071        13916 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCCM010000071 DACCCM010000000
VERSION     DACCCM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.075"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            131..505
                     /locus_tag="IVK78_002158"
     CDS             131..505
                     /locus_tag="IVK78_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6375607.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVK78_002159"
     CDS             complement(619..1248)
                     /locus_tag="IVK78_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6375608.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVK78_002160"
     tRNA            complement(1382..1454)
                     /locus_tag="IVK78_002160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVK78_002161"
     tRNA            1668..1741
                     /locus_tag="IVK78_002161"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVK78_002162"
     CDS             1696..1845
                     /locus_tag="IVK78_002162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375609.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IVK78_002163"
     CDS             2338..3666
                     /locus_tag="IVK78_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6375610.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IVK78_002164"
     CDS             3868..4701
                     /locus_tag="IVK78_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP6375611.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IVK78_002165"
     CDS             4717..5454
                     /locus_tag="IVK78_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP6375612.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IVK78_002166"
     CDS             complement(5609..6577)
                     /locus_tag="IVK78_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP6375613.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IVK78_002167"
     CDS             6809..7642
                     /locus_tag="IVK78_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP6375614.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7654..8514
                     /locus_tag="IVK78_002168"
     CDS             7654..8514
                     /locus_tag="IVK78_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6375615.1"
                     /translation="MQERGEVYDQSYFFDIDGTLVTNRSKALESTKQAIEYARKNGIL
                     CGVATGRSPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADE
                     NHRQIVFGARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDR
                     YETLEILKEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQA
                     FANAVEIEMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIY
                     EGLKHFGLIY"
     gene            8540..9100
                     /locus_tag="IVK78_002169"
     CDS             8540..9100
                     /locus_tag="IVK78_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6375616.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9299..11020
                     /locus_tag="IVK78_002170"
     CDS             9299..11020
                     /locus_tag="IVK78_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP6375617.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11185..12327
                     /locus_tag="IVK78_002171"
     CDS             11185..12327
                     /locus_tag="IVK78_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6375618.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12343..13554
                     /gene="thiI"
                     /locus_tag="IVK78_002172"
     CDS             12343..13554
                     /gene="thiI"
                     /locus_tag="IVK78_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP6375619.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2297 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     7741 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     7801 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     7861 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     7921 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     7981 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     8041 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     8101 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     8161 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     8221 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     8281 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     8341 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     8401 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     8461 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     8521 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     8581 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     8641 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     8701 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     8761 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     8821 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     8881 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     8941 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     9001 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     9061 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     9121 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     9181 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     9241 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     9301 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     9361 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     9421 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     9481 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     9541 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     9601 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     9661 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     9721 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     9781 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     9841 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     9901 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     9961 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
    10021 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
    10081 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
    10141 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
    10201 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
    10261 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
    10321 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
    10381 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
    10441 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
    10501 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
    10561 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
    10621 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
    10681 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
    10741 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
    10801 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
    10861 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
    10921 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
    10981 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
    11041 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
    11101 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
    11161 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
    11221 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
    11281 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
    11341 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
    11401 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
    11461 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
    11521 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
    11581 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
    11641 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
    11701 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    11761 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    11821 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    11881 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    11941 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    12001 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    12061 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    12121 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    12181 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    12241 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    12301 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    12361 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    12421 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    12481 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    12541 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    12601 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    12661 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    12721 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    12781 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    12841 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    12901 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    12961 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    13021 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    13081 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    13141 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    13201 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    13261 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aattattgaa
    13321 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    13381 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    13441 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    13501 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    13561 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    13621 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    13681 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    13741 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    13801 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    13861 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttg
//
LOCUS       DACCCM010000072        13488 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCCM010000072 DACCCM010000000
VERSION     DACCCM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.076"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(164..388)
                     /locus_tag="IVK78_002173"
     CDS             complement(164..388)
                     /locus_tag="IVK78_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP6375620.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="IVK78_002174"
     CDS             complement(586..2166)
                     /locus_tag="IVK78_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP6375621.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="IVK78_002175"
     CDS             complement(2567..3943)
                     /locus_tag="IVK78_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6375622.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="IVK78_002176"
     CDS             complement(4071..5189)
                     /locus_tag="IVK78_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6375623.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="IVK78_002177"
     CDS             5331..5765
                     /locus_tag="IVK78_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6375624.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="IVK78_002178"
     CDS             complement(5843..6376)
                     /locus_tag="IVK78_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP6375625.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IVK78_002179"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IVK78_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP6375626.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IVK78_002180"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IVK78_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP6375627.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="IVK78_002181"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="IVK78_002181"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6375628.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="IVK78_002182"
     CDS             complement(10101..10679)
                     /locus_tag="IVK78_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP6375629.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="IVK78_002183"
     CDS             11001..11471
                     /locus_tag="IVK78_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP6375630.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="IVK78_002184"
     CDS             complement(11579..12805)
                     /locus_tag="IVK78_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP6375631.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3959 a         2719 c         2248 g         4562 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaa
//
LOCUS       DACCCM010000073        13427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCCM010000073 DACCCM010000000
VERSION     DACCCM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.077"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..912)
                     /locus_tag="IVK78_002185"
     CDS             complement(<1..912)
                     /locus_tag="IVK78_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP6375632.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="IVK78_002186"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="IVK78_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP6375633.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="IVK78_002187"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="IVK78_002187"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP6375634.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="IVK78_002188"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="IVK78_002188"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP6375635.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="IVK78_002189"
     CDS             4438..5319
                     /locus_tag="IVK78_002189"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP6375636.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="IVK78_002190"
     CDS             5316..6032
                     /locus_tag="IVK78_002190"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP6375637.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="IVK78_002191"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="IVK78_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP6375638.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="IVK78_002192"
     CDS             6749..7750
                     /locus_tag="IVK78_002192"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP6375639.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="IVK78_002193"
     CDS             7747..9108
                     /locus_tag="IVK78_002193"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP6375640.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="IVK78_002194"
     CDS             9068..10219
                     /locus_tag="IVK78_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP6375641.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="IVK78_002195"
     CDS             10194..11363
                     /locus_tag="IVK78_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6375642.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="IVK78_002196"
     CDS             11538..12917
                     /locus_tag="IVK78_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6375643.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="IVK78_002197"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="IVK78_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4483 a         2339 c         2923 g         3682 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gagaaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DACCCM010000074        13404 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCCM010000074 DACCCM010000000
VERSION     DACCCM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.078"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            79..1125
                     /locus_tag="IVK78_002198"
     CDS             79..1125
                     /locus_tag="IVK78_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6375644.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IVK78_002199"
     CDS             1474..2106
                     /locus_tag="IVK78_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP6375645.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IVK78_002200"
     CDS             2694..4133
                     /locus_tag="IVK78_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375646.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IVK78_002201"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IVK78_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IVK78_002202"
     CDS             4895..5110
                     /locus_tag="IVK78_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375647.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IVK78_002203"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IVK78_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IVK78_002204"
     CDS             complement(5770..6681)
                     /locus_tag="IVK78_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6375648.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IVK78_002205"
     CDS             complement(6700..7704)
                     /locus_tag="IVK78_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6375649.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IVK78_002206"
     CDS             complement(7701..9824)
                     /locus_tag="IVK78_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6375650.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IVK78_002207"
     CDS             complement(9829..12276)
                     /locus_tag="IVK78_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6375651.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IVK78_002208"
     CDS             complement(12263..12652)
                     /locus_tag="IVK78_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6375652.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IVK78_002209"
     CDS             complement(12712..13215)
                     /locus_tag="IVK78_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6375653.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..>13404)
                     /locus_tag="IVK78_002210"
     CDS             complement(13228..>13404)
                     /locus_tag="IVK78_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375654.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4099 a         2643 c         2454 g         4208 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaacccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DACCCM010000075        13346 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCCM010000075 DACCCM010000000
VERSION     DACCCM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.079"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..1364)
                     /locus_tag="IVK78_002211"
     CDS             complement(45..1364)
                     /locus_tag="IVK78_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6375655.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IVK78_002212"
     CDS             complement(1361..2014)
                     /locus_tag="IVK78_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6375656.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IVK78_002213"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IVK78_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IVK78_002214"
     CDS             complement(2724..3440)
                     /locus_tag="IVK78_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP6375657.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="IVK78_002215"
     CDS             complement(3564..3749)
                     /locus_tag="IVK78_002215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375658.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="IVK78_002216"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IVK78_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IVK78_002217"
     CDS             complement(5356..6387)
                     /locus_tag="IVK78_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375659.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IVK78_002218"
     CDS             complement(6394..7224)
                     /locus_tag="IVK78_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6375660.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IVK78_002219"
     CDS             complement(7221..8027)
                     /locus_tag="IVK78_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6375661.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IVK78_002220"
     CDS             complement(8033..8857)
                     /locus_tag="IVK78_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP6375662.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IVK78_002221"
     CDS             complement(8847..9947)
                     /locus_tag="IVK78_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6375663.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IVK78_002222"
     CDS             10125..10910
                     /locus_tag="IVK78_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP6375664.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IVK78_002223"
     CDS             10943..11326
                     /locus_tag="IVK78_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6375665.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IVK78_002224"
     CDS             11402..11533
                     /locus_tag="IVK78_002224"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6375666.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IVK78_002225"
     CDS             11502..11738
                     /locus_tag="IVK78_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6375667.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IVK78_002226"
     CDS             complement(11816..12787)
                     /locus_tag="IVK78_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP6375668.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4056 a         2911 c         2305 g         4074 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atgtcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DACCCM010000076        13183 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCCM010000076 DACCCM010000000
VERSION     DACCCM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.080"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK78_002227"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK78_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6375669.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IVK78_002228"
     CDS             1995..4661
                     /locus_tag="IVK78_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6375670.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IVK78_002229"
     CDS             5025..5777
                     /locus_tag="IVK78_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6375671.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IVK78_002230"
     CDS             complement(5799..6953)
                     /locus_tag="IVK78_002230"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP6375672.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IVK78_002231"
     CDS             7138..9579
                     /locus_tag="IVK78_002231"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP6375673.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IVK78_002232"
     CDS             complement(9909..10613)
                     /locus_tag="IVK78_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375674.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IVK78_002233"
     CDS             complement(10800..11045)
                     /locus_tag="IVK78_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375675.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IVK78_002234"
     CDS             complement(11062..11913)
                     /locus_tag="IVK78_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6375676.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IVK78_002235"
     CDS             12079..13083
                     /locus_tag="IVK78_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP6375677.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4209 a         2341 c         2754 g         3879 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtg
//
LOCUS       DACCCM010000077        13114 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCCM010000077 DACCCM010000000
VERSION     DACCCM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.081"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(68..1747)
                     /locus_tag="IVK78_002236"
     CDS             complement(68..1747)
                     /locus_tag="IVK78_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6375678.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            2295..3797
                     /locus_tag="IVK78_002237"
     CDS             2295..3797
                     /locus_tag="IVK78_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6375679.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNLKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3810..4472
                     /locus_tag="IVK78_002238"
     CDS             3810..4472
                     /locus_tag="IVK78_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375680.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4573..4776)
                     /locus_tag="IVK78_002239"
     CDS             complement(4573..4776)
                     /locus_tag="IVK78_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6375681.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(4887..6071)
                     /gene="iadA"
                     /locus_tag="IVK78_002240"
     CDS             complement(4887..6071)
                     /gene="iadA"
                     /locus_tag="IVK78_002240"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP6375682.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(6228..7967)
                     /gene="yfcC"
                     /locus_tag="IVK78_002241"
     CDS             complement(6228..7967)
                     /gene="yfcC"
                     /locus_tag="IVK78_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6375683.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(8740..9354)
                     /locus_tag="IVK78_002242"
     CDS             complement(8740..9354)
                     /locus_tag="IVK78_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6375684.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(9960..10337)
                     /locus_tag="IVK78_002243"
     CDS             complement(9960..10337)
                     /locus_tag="IVK78_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375685.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(10637..11518)
                     /gene="galU"
                     /locus_tag="IVK78_002244"
     CDS             complement(10637..11518)
                     /gene="galU"
                     /locus_tag="IVK78_002244"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6375686.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(11561..12580)
                     /locus_tag="IVK78_002245"
     CDS             complement(11561..12580)
                     /locus_tag="IVK78_002245"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6375687.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3890 a         2771 c         2207 g         4246 t
ORIGIN      
        1 tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt catcttactc
       61 tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc aagatcactg
      121 ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg tcttgcttta
      181 atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt tcaatcatat
      241 tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc cgtgaagtaa
      301 tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc gctacgaaaa
      361 tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata tcacctatgc
      421 ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc atacgcttgt
      481 tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat ggcatcccta
      541 ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga actggcacaa
      601 aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg gcatggccag
      661 aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa atgatatctt
      721 ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata taaaccaaat
      781 ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc agagacttga
      841 tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca tagtttttca
      901 tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct aaacgcatcg
      961 ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt tgtgcttttt
     1021 ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa gcgacgatga
     1081 tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg gcttcagaag
     1141 ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag actccttctt
     1201 tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc gtttggtcaa
     1261 atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg atccccactg
     1321 aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca aagtctattt
     1381 ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca ttacgactga
     1441 cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca gaaatttttg
     1501 acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa aagacaccag
     1561 ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg tcgatcccca
     1621 acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc tcgcccactt
     1681 ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata attttaatgg
     1741 tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga ttttcttctt
     1801 tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt ctcaacttat
     1861 ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat gggcatttta
     1921 ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc cagaatactt
     1981 atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc gctagtatca
     2041 gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg atttcttttg
     2101 ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat tttaccacat
     2161 tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga tggtgtgcaa
     2221 acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg catctactag
     2281 gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa gtaaaatggg
     2341 gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg ctagctggcc
     2401 tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa gttggagcaa
     2461 cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa acttcttcaa
     2521 gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca ccagtttcct
     2581 taacggacgc ggaaaaaata gctgaattag ataatttaaa gagttattcc tttgaaagtt
     2641 ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat tcaagtgaag
     2701 acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc gggaatccgc
     2761 aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa aatagtaatt
     2821 tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc gacaaaggca
     2881 caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca gtcggagata
     2941 ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg atcaaaggaa
     3001 tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc atgaatccgt
     3061 cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca tctgacgata
     3121 acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat gagtttctca
     3181 aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat gattccatgt
     3241 atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat gttgttttac
     3301 tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc attcgggagc
     3361 gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa atcattctcc
     3421 aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct tcgcttagcg
     3481 gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca gaaaccacga
     3541 cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa gaacaaggcg
     3601 gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct gatcaagtca
     3661 gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga gtagcatttg
     3721 gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta aacccaagaa
     3781 aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg ggattttggt
     3841 atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa gctggaaaaa
     3901 tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg cttatttctg
     3961 gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg aacaaaatcg
     4021 ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac aacttgcttc
     4081 cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag accaagcaag
     4141 agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat gaaatattag
     4201 caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct atcgttcgtg
     4261 ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat ctagatgaaa
     4321 caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa aatcgctgta
     4381 ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc tttgaactaa
     4441 aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca aataaaaaat
     4501 ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga ttcaattagt
     4561 aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga cttcgtcgcc
     4621 taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc cttctgtttc
     4681 tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga ctactttatc
     4741 tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc ttagtttgtt
     4801 tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa cgctcgtttt
     4861 ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc ccatactttg
     4921 acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat atcaagcgtt
     4981 tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc ttttttatcc
     5041 aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc caaagggata
     5101 ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact tcctactcct
     5161 aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc atctgaggtc
     5221 atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc tttgctgaag
     5281 cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt gaagtcaatc
     5341 gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt gcgatttgca
     5401 tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg tgtcaatagc
     5461 tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt tccggataac
     5521 atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact tggctgagaa
     5581 gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat tgtcaaaaag
     5641 tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc ttcgtataca
     5701 taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc agtcatatct
     5761 ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc tgtagtcaat
     5821 tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc tccacctaaa
     5881 atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc tgaaagttct
     5941 tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa aatcttccca
     6001 ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc aatttgtcga
     6061 atgattttca tacatttttc ctctttcttt aagtataact gatgatagga tcttaaaaaa
     6121 taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg gttcttggag
     6181 tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta ctgcagacct
     6241 attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc ttgcatcttg
     6301 atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac accaatcaag
     6361 ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata agcaagaact
     6421 gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg catagtcaaa
     6481 gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa gatgtacatc
     6541 gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc atgcaaaatc
     6601 gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag aatccctttg
     6661 gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg gaaagaagaa
     6721 gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat tccagcaact
     6781 aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt ttgagtcacg
     6841 ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt atgcccaagc
     6901 atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata atcatctgat
     6961 ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat catcatataa
     7021 ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc accagacaac
     7081 acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat ccaagaggtc
     7141 gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga gtcatatcct
     7201 aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc tgccatcccg
     7261 aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa acgctccagt
     7321 cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt acgcataata
     7381 ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc agatccatac
     7441 ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc gccaaaatca
     7501 tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata gatattttcg
     7561 ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt ttcctgcgta
     7621 agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac atccagtttt
     7681 tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata cgacttatcc
     7741 agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact tgtttcgcca
     7801 tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac gacaaaagtc
     7861 agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg tgtcgataat
     7921 ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt tatttcttcc
     7981 tttctttttt cagtaatttt gatgaataaa aaatggataa tcaaatgaat tatacagtaa
     8041 aaatgacatt tccacatgaa taatttatta gaaaatcact tttttctatt ttgttttttt
     8101 tctcctatgt atgattattt ttttctcttt ccatttatat taagtatttt atttctctgt
     8161 ttttccttaa aattcttttt ttatttttag attagagaaa atctcgtcct gtttttttca
     8221 ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca cttataaata
     8281 tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag gtatcctgtt
     8341 ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat gtactataat
     8401 tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga gacaagaaag
     8461 aatccatatg gattctttct tgtctctttt tccactactt tttatttttc tgacactaca
     8521 tattcttata actcatttct cataactgtg aaacaactta taaaaataac aaactttaaa
     8581 caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa aaaaagtagt
     8641 cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt tttatgtgga
     8701 acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac ttgtgtcctg
     8761 gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga
     8821 ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat catgtaaatt
     8881 ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg
     8941 cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat gaccattttc
     9001 tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg aaagaagaag
     9061 aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg caccttctag
     9121 cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa gatccggggt
     9181 ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata tctgcaatct
     9241 accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca gcggctcttc
     9301 tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac tcatttatct
     9361 ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag gaagtacata
     9421 aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca caaaaaaggc
     9481 attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca gaccatggaa
     9541 aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt aggtcgatgc
     9601 actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg gtcattccct
     9661 gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt tctatctgaa
     9721 tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa acattggtat
     9781 gatttatata tatataaata acataaaaaa aggaatggct tatcccgata caacaagcat
     9841 tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca attaaataaa
     9901 tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc tactaaatat
     9961 taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga aaaagtagtt
    10021 gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat ataaatgatt
    10081 agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc taacgccaat
    10141 tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat cagaaacgtt
    10201 tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag tggcaacaca
    10261 aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga ctgtgcaaca
    10321 gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa ttcgcctttt
    10381 tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca ttttctttta
    10441 tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat tcacgaaagt
    10501 ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa ctaaaacgac
    10561 agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc attctcagca
    10621 ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg ataatatatg
    10681 cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt gtcttcatat
    10741 atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct
    10801 ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct gctcctggtt
    10861 cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg ataatggcta
    10921 aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact ctgcaacgac
    10981 ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca ttttctggta
    11041 cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc aattgttgag
    11101 tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac ggttcatttc
    11161 ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga gattgacgga
    11221 tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct gttttttcct
    11281 tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca atgggtcttt
    11341 tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct tcttccacaa
    11401 taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg gcttttgttg
    11461 ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg accttcactt
    11521 gctgttcctc ctaaacgacg attaactatt gatcatctat ttaacgaaga gaaaattcat
    11581 tctccatttt tccgtcacgt aacatgattt cttttgctgc ttttttgata tcttctccat
    11641 tatacaaaac attatagatt gtttgagtaa ttggcatctc aacacctagc tgttgagcta
    11701 gctctactgc tgcttttgtc gttgccacac cctcaacgac cattcccatg ttttctagaa
    11761 cttcttccag cttatgtccc tgtcctaaca agttaccggc acgccaattg cgagaatgaa
    11821 cacttgtaca agtaacgatc aagtccccca caccactcaa accgataaat gtcagaggat
    11881 tggcacccat cgcgacacca agacggctga tttctgccaa tcctcgagtc atgattgctg
    11941 cttttgcatc atctccgaat cctaatccat ggatagcgcc ggcaccaatt gcaatgatat
    12001 ttttcagtgc tgcaccagtt tctacgccaa tcacatcatt atttgtataa atcctgaaat
    12061 aatcattcat gaacagtttt tgtacatagg ctgctgcttt ttgatcaata cttgcagctg
    12121 taattgttgt gatatcatgt actgccactt cctcagcatg gctaggacca gataatacaa
    12181 caattgcctg tcttttttct tctggaattt cttcagccaa aacttctgag attcgtttat
    12241 gcgtatcttg ttctagtcct ttacttgcat gtataatgat tggcttagta ttcattttac
    12301 caataagttc ctgtgcgact gaacgaatcg cttttgttgg aacaacaaat aaaatagcat
    12361 ctgcgttatc cactgcttct gccaattctt tatatccgat aatcgattct gggatcgtca
    12421 aatcaggtaa aaaatgctga tttgtatgat acttattgat ttcatcaatc tgttcaggta
    12481 catttcccca aatacggaca tcatgcccgt tttctgccaa tgtttgtgct aaagcagtgc
    12541 cccaagatcc aggaccaaga actgcgattt tttgtttcat ggatattcct ccaaataaat
    12601 aattgataat ttccattata gtatacattt cttaaagttg ggactaattt ccattgtttt
    12661 taagtgtttg tttacttctt ttatcgtttt tacttgaaat aatcaatgat atgttacaat
    12721 agtctacatt ttatttttac actcgttatg ctttgcttgt tcctaagagg aagcagtttt
    12781 gacaatgtta ctaccagcat tactattagg aggttaggaa acttctccta gtgagtattg
    12841 atttaaaata cataagagac agattgaaca tttctaaatc tgtcaggtcc tgcaacggac
    12901 agattgtctc tgttgacggt tgacatagga acttttgttt tagtcaacat tctggataca
    12961 aaacgctatg taaactggcg caactcgttg tacttggact tttactacgg atccatgcaa
    13021 caactaaata gtactattgc ataacaaaaa atggttggca cccacatgtc ccattaaaca
    13081 gattataagt tgaactgtag ataatgcaaa aaat
//
LOCUS       DACCCM010000078        13049 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCCM010000078 DACCCM010000000
VERSION     DACCCM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.082"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(77..149)
                     /locus_tag="IVK78_002246"
     tRNA            complement(77..149)
                     /locus_tag="IVK78_002246"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IVK78_002247"
     CDS             530..1099
                     /locus_tag="IVK78_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP6375688.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IVK78_002248"
     CDS             1086..1946
                     /locus_tag="IVK78_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6375689.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IVK78_002249"
     CDS             2136..2840
                     /locus_tag="IVK78_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6375690.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IVK78_002250"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IVK78_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP6375691.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IVK78_002251"
     CDS             4677..5993
                     /locus_tag="IVK78_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375692.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IVK78_002252"
     CDS             5994..6863
                     /locus_tag="IVK78_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375693.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IVK78_002253"
     CDS             6918..7727
                     /locus_tag="IVK78_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6375694.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IVK78_002254"
     CDS             complement(7830..8459)
                     /locus_tag="IVK78_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP6375695.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IVK78_002255"
     CDS             8629..9921
                     /locus_tag="IVK78_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6375696.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IVK78_002256"
     CDS             complement(9997..10659)
                     /locus_tag="IVK78_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6375697.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IVK78_002257"
     CDS             10884..11168
                     /locus_tag="IVK78_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP6375698.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IVK78_002258"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IVK78_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP6375699.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4338 a         2254 c         2824 g         3633 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttctt
//
LOCUS       DACCCM010000079        12597 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCCM010000079 DACCCM010000000
VERSION     DACCCM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.083"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            555..755
                     /locus_tag="IVK78_002259"
     CDS             555..755
                     /locus_tag="IVK78_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6375700.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1323..1886
                     /locus_tag="IVK78_002260"
     CDS             1323..1886
                     /locus_tag="IVK78_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375701.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1947..2291)
                     /locus_tag="IVK78_002261"
     CDS             complement(1947..2291)
                     /locus_tag="IVK78_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375702.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2388..2633)
                     /locus_tag="IVK78_002262"
     CDS             complement(2388..2633)
                     /locus_tag="IVK78_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6375703.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2703..2957)
                     /locus_tag="IVK78_002263"
     CDS             complement(2703..2957)
                     /locus_tag="IVK78_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375704.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3121..3444)
                     /locus_tag="IVK78_002264"
     CDS             complement(3121..3444)
                     /locus_tag="IVK78_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375705.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3631..3704
                     /locus_tag="IVK78_002265"
     tRNA            3631..3704
                     /locus_tag="IVK78_002265"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3665..3667,aa:Ile,seq:cat)
     gene            complement(4089..4586)
                     /locus_tag="IVK78_002266"
     CDS             complement(4089..4586)
                     /locus_tag="IVK78_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6375706.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4626..5435)
                     /locus_tag="IVK78_002267"
     CDS             complement(4626..5435)
                     /locus_tag="IVK78_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6375707.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5769..6626
                     /locus_tag="IVK78_002268"
     CDS             5769..6626
                     /locus_tag="IVK78_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6375708.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6619..7521
                     /locus_tag="IVK78_002269"
     CDS             6619..7521
                     /locus_tag="IVK78_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6375709.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7554..7904
                     /locus_tag="IVK78_002270"
     CDS             7554..7904
                     /locus_tag="IVK78_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6375710.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7938..8876
                     /locus_tag="IVK78_002271"
     CDS             7938..8876
                     /locus_tag="IVK78_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6375711.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8954..9847
                     /locus_tag="IVK78_002272"
     CDS             8954..9847
                     /locus_tag="IVK78_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6375712.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9885..10448
                     /gene="amaP"
                     /locus_tag="IVK78_002273"
     CDS             9885..10448
                     /gene="amaP"
                     /locus_tag="IVK78_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6375713.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10462..10650
                     /locus_tag="IVK78_002274"
     CDS             10462..10650
                     /locus_tag="IVK78_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6375714.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10663..11565
                     /locus_tag="IVK78_002275"
     CDS             10663..11565
                     /locus_tag="IVK78_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6375715.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11597..11839
                     /locus_tag="IVK78_002276"
     CDS             11597..11839
                     /locus_tag="IVK78_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6375716.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12095..12466)
                     /locus_tag="IVK78_002277"
     CDS             complement(12095..12466)
                     /locus_tag="IVK78_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375717.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4461 a         2113 c         2366 g         3657 t
ORIGIN      
        1 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
       61 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
      121 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
      181 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
      241 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
      301 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
      361 aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat
      421 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
      481 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
      541 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
      601 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
      661 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
      721 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
      781 tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt ggtcgttgaa tgggctgaat
      841 agtagtgtat aaaacaattt tgtggagcgt gaagctttta tggtagagga aaatacgaat
      901 aaagtgatgt tgataaaaag attcctatct tgaatatgaa tcgagatgtc ttgaaaatga
      961 atgagtttga ccaatcatta tcctatcgaa atttacagtt tcgttcgatt ggtctgtcaa
     1021 ttaatgaaaa agtaaaaaag aatatgttct aggaagaggg cggcacatct aaatttttaa
     1081 gtaacggaca gttcaaatat caagcattga aagaggctat cctactgttt gaaggaatac
     1141 gaatatataa aaacgacaaa gaggaaaaag tagttcatta aatcccttca agtgatgaaa
     1201 gaatgagatc taataaaacc tgatctttca tctgtttttt taataacgta ataatttttt
     1261 tattttttgt gctttcgccg ttattgtaga gtgatttagc attcttattt ttaaaaataa
     1321 ctgtgttata tattaaaaaa ggcataatag ttataagctt ttttaaagaa tatttagaaa
     1381 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
     1441 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
     1501 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
     1561 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
     1621 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
     1681 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
     1741 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
     1801 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
     1861 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
     1921 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
     1981 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
     2041 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
     2101 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
     2161 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
     2221 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
     2281 tttgaaatca atttttcatg ccataatcac atagaaatgt ctcctttata tatagattgt
     2341 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     2401 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     2461 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     2521 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     2581 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     2641 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     2701 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     2761 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     2821 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     2881 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     2941 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     3001 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     3061 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     3121 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     3181 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     3241 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     3301 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     3361 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     3421 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     3481 aagcaaataa aaattaaagt aacaaaatag ttatgacaca agtaaaagaa ataagaataa
     3541 caatattatt ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga
     3601 aaagtaacaa aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac
     3661 ggctcataac cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa
     3721 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact
     3781 tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga
     3841 aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg
     3901 ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt
     3961 gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg
     4021 taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat
     4081 agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat
     4141 tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa
     4201 gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact
     4261 tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt
     4321 tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga
     4381 agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata
     4441 tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata
     4501 cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga
     4561 ttttgcatat caataataac taacattttc tttcctccta cattcatatc gtcattttat
     4621 tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt
     4681 acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg
     4741 ctgtaattca acatatcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat
     4801 tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc
     4861 gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct
     4921 ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta
     4981 agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa
     5041 tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct
     5101 tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt
     5161 ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc
     5221 atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa
     5281 aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt
     5341 tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc
     5401 aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca
     5461 tatctatttt cctgttacct taagattaaa tgagaattta aaaaaatgaa actaacttgc
     5521 tttgtcatta tgaatttttt caaaaaacat tcttaagaaa aaggtggcta tatatagtag
     5581 tttttggtca atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat
     5641 gcgttttgtg ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt
     5701 aaaaccaaat gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag
     5761 tgattttgat ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt
     5821 atgctcctta cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac
     5881 gcataaaatt ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag
     5941 aaacggtaaa taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata
     6001 tctacgaaga cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt
     6061 taccaaactt attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt
     6121 tagtactaga cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg
     6181 ctattatcga tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact
     6241 tgtacttcaa ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta
     6301 cagaaaaaac tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag
     6361 atgctttgaa tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc
     6421 agacttcgct gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata
     6481 aagaaggaaa tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata
     6541 cgttgaaaga tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa
     6601 cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca
     6661 aaattcgtac cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct
     6721 aaatttgttc ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta
     6781 cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa
     6841 gaattcttta caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt
     6901 gcgattccgg aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac
     6961 atgatcgctt tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta
     7021 gcagctgttg aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa
     7081 tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa
     7141 aatattacgc aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat
     7201 gaccaagatg cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg
     7261 aatgcacaag gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt
     7321 gaatacgaag aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg
     7381 tggagcaagg attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg
     7441 gctttccgtt gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa
     7501 acagatgtag ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa
     7561 ttacaatgtc taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa
     7621 tcgcactcgc gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa
     7681 aaggcagctt ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa
     7741 tcgagcatcc attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac
     7801 gattgaacat ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag
     7861 tactcgatta taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc
     7921 caaaaaggag gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa
     7981 cagatgaaaa aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc
     8041 aagcaatcga agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag
     8101 aagtttctcg gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag
     8161 tgattaatct agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg
     8221 tactttccga taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc
     8281 tgctgacaaa gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat
     8341 tagatattta cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt
     8401 tggataaagc agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg
     8461 aaagagacat cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat
     8521 tatggaattc tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc
     8581 atttgagaca gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta
     8641 ttggtgatct attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag
     8701 attcagctgc attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata
     8761 caggaggatt accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt
     8821 tgatagcagc agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa
     8881 tctatttaaa acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag
     8941 gaggaataca gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga
     9001 gaaatttcct aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc
     9061 tgattgtgga gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat
     9121 cactggtgga gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc
     9181 agatatcgct cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata
     9241 cgtgaaagtt gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc
     9301 acctaaagaa atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact
     9361 aaacgcagga cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa
     9421 agacacattt atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca
     9481 tatcccagca ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga
     9541 acatttatta gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc
     9601 aaaacagctt gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatctggac
     9661 accattacaa ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag
     9721 tttactagaa cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc
     9781 gaataaagca agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa
     9841 cttataagaa gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa
     9901 aaacgatcgg agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat
     9961 taagtgcaac ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta
    10021 attcatacgt gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta
    10081 tcacgttact tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg
    10141 gtgatgggga gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc
    10201 atgaagatga attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg
    10261 ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat
    10321 tgatgaaaga aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag
    10381 tatcggtgaa atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag
    10441 tagaataggg ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc
    10501 tttggggtac ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc
    10561 ttattgatct tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg
    10621 aaaatcttag atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa
    10681 aataataaca ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca
    10741 ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat
    10801 ccagatccag tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa
    10861 aagcatgcac ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca
    10921 atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa
    10981 gaaaaactta gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat
    11041 aaccaaggaa agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa
    11101 gataaagttg tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct
    11161 gcaaacggtg gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg
    11221 gctgggatcg aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg
    11281 gaatatggca aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa
    11341 gtaaaaaata tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa
    11401 acaaaagaag aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg
    11461 aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc
    11521 ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaaca
    11581 ttaggaggaa cgaaacatgg gatttatttg gtcacttatc gtcggaggaa ttatcggagc
    11641 aattgcagga gcaatcacga ataaaggttc ttcgatgggg attattgcaa atgtcgtagc
    11701 aggtttagtt ggatcagcac ttggtcaagc attattaggt tcttggggac caagtattgc
    11761 aggaatggca atcgtgccat cattgattgg tgccatcatt ttagtactaa tcgtatcatt
    11821 ctttgctcga aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc tgttcttcat
    11881 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggcata
    11941 ttatcgaaga tgcaagcttt tgaacactgt ttatacggaa taagaggctg tgacaagact
    12001 tttgtcacag cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc
    12061 ttaaatgtgt ctttattgat acagagaagc ttctttaata aaacaatgcc tccctaaatt
    12121 ttcttgagat aggaaaggtg aacatacaaa ataatacgac cactttgaaa acccagatga
    12181 tccacaaatg gatcgtatga tactgatgaa gtaaaaagca atggatcaga aactcaaaga
    12241 gattgaaaaa gacaaggaag aaataatatc tcttttttac tcttgtctgt tttagccaat
    12301 aatagctcgt aaagtaatct agacaaacaa atagaagtaa acaaagtttc gtaagagcag
    12361 caatcgtatc atgttgctct gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa
    12421 tatagatcac gagtaatgaa agaaaaaagt aaacaataaa tggcatacta ttcctcctaa
    12481 ttgtgtgagt tcgccctcat tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc
    12541 gtaacaaatg ttatctaatg tatttttaaa taaataatca ttattatcaa aataatg
//
LOCUS       DACCCM010000080        12416 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCCM010000080 DACCCM010000000
VERSION     DACCCM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.084"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..189)
                     /locus_tag="IVK78_002278"
     CDS             complement(<1..189)
                     /locus_tag="IVK78_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375718.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(199..1014)
                     /locus_tag="IVK78_002279"
     CDS             complement(199..1014)
                     /locus_tag="IVK78_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6375719.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1276..1719)
                     /locus_tag="IVK78_002280"
     CDS             complement(1276..1719)
                     /locus_tag="IVK78_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP6375720.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1853..2191)
                     /locus_tag="IVK78_002281"
     CDS             complement(1853..2191)
                     /locus_tag="IVK78_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6375721.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2179..2556)
                     /locus_tag="IVK78_002282"
     CDS             complement(2179..2556)
                     /locus_tag="IVK78_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6375722.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2780..3973
                     /locus_tag="IVK78_002283"
     CDS             2780..3973
                     /locus_tag="IVK78_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375723.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4139..4561)
                     /locus_tag="IVK78_002284"
     CDS             complement(4139..4561)
                     /locus_tag="IVK78_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6375724.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4956..5150
                     /locus_tag="IVK78_002285"
     CDS             4956..5150
                     /locus_tag="IVK78_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375725.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5147..5641
                     /locus_tag="IVK78_002286"
     CDS             5147..5641
                     /locus_tag="IVK78_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375726.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(5803..6975)
                     /locus_tag="IVK78_002287"
     CDS             complement(5803..6975)
                     /locus_tag="IVK78_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6375727.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7181..7996)
                     /locus_tag="IVK78_002288"
     CDS             complement(7181..7996)
                     /locus_tag="IVK78_002288"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6375728.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNMI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8146..8592)
                     /locus_tag="IVK78_002289"
     CDS             complement(8146..8592)
                     /locus_tag="IVK78_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6375729.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8589..9593)
                     /locus_tag="IVK78_002290"
     CDS             complement(8589..9593)
                     /locus_tag="IVK78_002290"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6375730.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9633..9962)
                     /locus_tag="IVK78_002291"
     CDS             complement(9633..9962)
                     /locus_tag="IVK78_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6375731.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10088..12415)
                     /locus_tag="IVK78_002292"
     CDS             complement(10088..12415)
                     /locus_tag="IVK78_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6375732.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3509 a         2424 c         2272 g         4211 t
ORIGIN      
        1 gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat
       61 cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact
      121 tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg
      181 ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata
      241 tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat
      301 gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt
      361 tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac
      421 gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg
      481 atagattccc cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat
      541 actactgtag tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac
      601 caacgaccac aacgtatatg ttaaacccac ttttctcatc ataatgaact gcgggatcat
      661 ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc
      721 tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa
      781 cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga
      841 ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt
      901 gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt
      961 caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt
     1021 tgaacgaatt tttgcttttt ttctttcatt tcgaacacct ccaaagttat aatcttcatt
     1081 tctttgtaat gattacaatc cagaacaacg gttattatag ctgattcatt tccgttcttc
     1141 ttatgtttgt aagcgcttat ttaacaagtt atgtaaatct ctttattttt tttgatttaa
     1201 aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc ttttgtattg aaatttcttc
     1261 gattagagat acttattact gttcaaaagc ttctttgaaa cggtgaatca attcattgac
     1321 tacaggtgat aaaatccgat tctttttcca gactagcatg cagttcgttt caatcattgg
     1381 gtcaagcgga acgaatgtca ctcgctttga aagatgactt acagcacctt ctatgactaa
     1441 agcagcacct acctgattct caattagcgc aaatacattg aaaatcaaat tgtacgttcc
     1501 tgtgatcagc aactgctcct tttttatgcc taaccagtca gcaaacattc gctgtatttc
     1561 tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt
     1621 ttttgctaaa acagaatttt ctaaaatgag caatccccat ttttcctttc ttggtaacaa
     1681 aatcgtttcg tatttctctg tatctactgg atctagcaaa atcgctaaat ctaacaggct
     1741 tttttctagt cactccgtga tatcatcact tgttccgctg tggatatgaa attgaacgct
     1801 gggataatca cggatcaact tttctagtaa tagtgccaat gtattcgaat tatcagcttc
     1861 cacacagccg atcgtaaatc ggccgctgaa taattgtttt tttctgttct cgaattcttg
     1921 agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga aaataacctg
     1981 cttctgtcaa gatcattttg cggttttcgc gtacaaacag ttctgttcct agttcctctt
     2041 ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag atgtagaact tctgcggctt
     2101 gagagattgt tccttcttct gcaattgtcc aaaaatattt tagtaagcgt aactccatag
     2161 cgatgctcct tgattttttt acttgataca ttccatttta tttctagata acacacgatc
     2221 atatttcttg atttttcctt gtaaacgttc caaacactct tgcatttcag caatttcttt
     2281 cgtcaaaagt tcactttctt caatcaaaag cttacgccgc tcaaatacag ttgcttctcc
     2341 ttcgctagat aggcgaatat attcaatcaa agactcgatc gataatccgg catttcgcat
     2401 acagatcgta aagtcgaccc attttaggtc ttcttctgta taatcacgga ttccgccttt
     2461 tgtccgatta actggtggaa tcaacccgat tcgttcataa tagcgcaatg tatcggccga
     2521 aatgcccttt tcttcactga cttgtttgat attcatctga tcactctttc tcttaatttc
     2581 ttgccacttt acttatgtat tttatttatt ctacaacttt aagtgtactc caagtcaata
     2641 ctcatttcta ttaaaactaa gctggattat ctatatcggt tgtttgtgta ttatttcaaa
     2701 taaaaaagtc ttgttttttt attctttttt ggatatactt ttaacaattt agggaaattt
     2761 taggaaaagg ggtataaaaa tgacaacgta tcagcgaaag gtattattat cgacatcggc
     2821 gggaattgct ttagagaata tggatattat gtttttagct ttttcattat cttcaatgat
     2881 tacttctttt catattagtg gtacgcaagc gggtttcatt tcaacgatca ctaatttggg
     2941 gatgctcatt ggaggtattt tttttggcat catagctgat aaatatggac gtgtgaaagt
     3001 attttctcaa acggtccttc tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa
     3061 catctacttg gtttatctct ttcgctttat cgcaggtgta ggagcgggag gagaatacgg
     3121 ggcgtgtatg tccttgattt ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc
     3181 ggttgctggt attggtgcgc aggtcggtgc ggctttggct gcgattttag ctgctgtgat
     3241 cattccaacg tttggttgga agatgttgta tgtcgtaggt gtactgccgg tgttgatggt
     3301 cttgttcatt cgtcgcggct tgactgaacc aaaggaattc aaatctgtac aatctgccga
     3361 aaagaaaacc aaacttagca gactgtttga atcaaaacat cttgcttggc aaacatgtgg
     3421 ccttagtatc atggtaactg tccaaattgc cggctatttt gggttaatga attggctgcc
     3481 ttcaattatg caaaatcgat tagggctttc ggtttctggg tcttcattgt ggatggtcag
     3541 tactatttta ggcatgtctt tggggatgat gacttttggc ataatcatgg ataaattggg
     3601 gcctaaacta gccttttctt tgtttttaat ttgttcatcc ttatctgtct atctacttgt
     3661 gttagcccat agccagtgga gtttggtagc agccgctgtg atagtcggct actttatcaa
     3721 tggcatgtat ggtggatatg gcgcagtgat tagtagtttg tatccaacag aaatccgcgc
     3781 gacggcgaat aatttcatta tgaaccttgg acgagctgtt ggtggttttt cttctatcgt
     3841 catcggtttc ttgatggatc attatagttt aactgccgta atcttgtttt tgagcggtat
     3901 ttacctagtc agtctgctgg tcttgacaac tcttactggt gttacagcat taagggaaag
     3961 cttgcagcat tgatagttga gtctgtaaat gacaagaaag aggtgtggca aaagcttgca
     4021 tctgcggtaa taagaccagc caaatcaaaa acatgagaca ctgtttccgt ttggctggtc
     4081 gtattacttg atgcagggca gctttttcaa ctgctaattt ccttctatct ggcggcgact
     4141 attctttgac gtccgtcatt tcaaagtact ttactaataa cttagcataa cgaaaaactg
     4201 tggatacact cgtatattga gcgtctgcat aatcttccaa atttttctca ggatgttgat
     4261 acatgaattc aatcaaatga taattatatg attttctgta gaaatgaaga taaataatgt
     4321 agattgggaa gttctcctgc ttttccatag aaaagcgccg attattctca ttcttcaaaa
     4381 tgaccttatc aggatatttt tggaacaatt cttctaaatc tgttagataa ttcaagatag
     4441 acttacttga taccttcaat tcgttttgta gttcttctat tgaacaccaa cgttctgttt
     4501 caagcagtaa atgaatcatt ttaattttct tttgagaaac ttttgttaaa aaagtctgca
     4561 tattttctcc tcctgtatct actttactat actacataca agaaaaaaaa cggatacaaa
     4621 tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta
     4681 agtattctat tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa
     4741 taaaataacg tacatatttt attattgcat acttaataac agaactatag caaaaaatcc
     4801 cgtgtatcaa cacgggatta gattaagttt cctcaatcac tattcataac acaattatta
     4861 taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag
     4921 gaggaaattt agcgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg
     4981 ggtgtggtgc tactacgtct actactcaaa gcacaagttc agatcaggct tccagcagca
     5041 gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga
     5101 aaaaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga
     5161 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
     5221 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
     5281 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
     5341 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
     5401 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaatcag cagctgaaca
     5461 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
     5521 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
     5581 cactttaagc gaagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
     5641 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagatggc
     5701 gttccagata gtaaaccaaa aataacagtt tacacgtcca tttcgtcagc ttctctcttt
     5761 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     5821 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     5881 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     5941 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     6001 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     6061 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     6121 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     6181 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     6241 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     6301 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     6361 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     6421 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     6481 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     6541 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     6601 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     6661 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     6721 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     6781 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     6841 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     6901 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     6961 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
     7021 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
     7081 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
     7141 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
     7201 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
     7261 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
     7321 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
     7381 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
     7441 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
     7501 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
     7561 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
     7621 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
     7681 tttctacagt aatcatgtta tccgtagttg tattttcccg attgactaca ccgtttggat
     7741 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
     7801 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
     7861 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
     7921 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
     7981 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
     8041 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
     8101 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
     8161 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
     8221 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
     8281 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
     8341 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
     8401 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
     8461 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
     8521 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
     8581 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
     8641 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
     8701 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
     8761 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
     8821 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
     8881 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
     8941 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
     9001 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
     9061 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
     9121 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
     9181 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
     9241 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
     9301 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
     9361 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
     9421 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
     9481 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
     9541 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
     9601 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
     9661 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
     9721 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
     9781 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
     9841 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
     9901 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
     9961 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
    10021 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
    10081 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
    10141 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
    10201 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
    10261 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
    10321 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
    10381 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
    10441 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
    10501 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
    10561 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
    10621 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
    10681 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
    10741 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
    10801 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
    10861 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
    10921 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
    10981 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
    11041 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
    11101 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
    11161 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
    11221 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
    11281 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
    11341 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
    11401 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
    11461 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
    11521 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
    11581 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
    11641 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
    11701 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
    11761 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
    11821 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
    11881 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
    11941 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
    12001 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
    12061 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
    12121 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
    12181 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
    12241 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
    12301 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
    12361 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatc
//
LOCUS       DACCCM010000081        12050 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCCM010000081 DACCCM010000000
VERSION     DACCCM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.085"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..208
                     /locus_tag="IVK78_002293"
     CDS             <1..208
                     /locus_tag="IVK78_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6375733.1"
                     /translation="EQYGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYIN
                     ERSFITDWKIIFLTLQAVLFGYGE"
     gene            237..1550
                     /locus_tag="IVK78_002294"
     CDS             237..1550
                     /locus_tag="IVK78_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP6375734.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            2356..3123
                     /locus_tag="IVK78_002295"
     CDS             2356..3123
                     /locus_tag="IVK78_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP6375735.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            3150..4151
                     /locus_tag="IVK78_002296"
     CDS             3150..4151
                     /locus_tag="IVK78_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP6375736.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            4174..5460
                     /locus_tag="IVK78_002297"
     CDS             4174..5460
                     /locus_tag="IVK78_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAP6375737.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            5494..6147
                     /locus_tag="IVK78_002298"
     CDS             5494..6147
                     /locus_tag="IVK78_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6375738.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            6180..7385
                     /locus_tag="IVK78_002299"
     CDS             6180..7385
                     /locus_tag="IVK78_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6375739.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            7515..8840
                     /locus_tag="IVK78_002300"
     CDS             7515..8840
                     /locus_tag="IVK78_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375740.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            8857..9987
                     /locus_tag="IVK78_002301"
     CDS             8857..9987
                     /locus_tag="IVK78_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375741.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            10013..11098
                     /locus_tag="IVK78_002302"
     CDS             10013..11098
                     /locus_tag="IVK78_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAP6375742.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     GTLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            11121..11936
                     /locus_tag="IVK78_002303"
     CDS             11121..11936
                     /locus_tag="IVK78_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375743.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
BASE COUNT         3959 a         1683 c         2692 g         3716 t
ORIGIN      
        1 ggaacaatac ggtgactacg aaaggcagcg gttgttcgta cagcctggtc ttagttgcta
       61 ttggcagatt gctccacatc gtaatgaact gtcctttgaa gaatgggtgg aacttgatgt
      121 gaagtacatc aatgaaagaa gctttatcac ggactggaag attatcttct tgactttaca
      181 ggctgtatta tttggatatg gtgaatgatt atatatagta ttaggagttg aaatatgtga
      241 atattatttt attatcgggt ggtagcggta ggcggttatg gccgctgtcc aacgatattc
      301 gtagcaaaca attcatcaaa ctgtttaagg atgaaaatgg cgaatatgaa tctatgattc
      361 aaagagttta tcgccagatt acaactgttg acccagatgc aaaggtcact attgcaacaa
      421 gtaagtctca ggctagtgca atccataatc agttgggaga taaggcttcg atttgtgtag
      481 aaccttgtcg tagagatact ttccctgcaa ttttgttagc tgcagcatat cttcacgatg
      541 aacttggcgt aggtgatgat gaaagtgttg cggtttgtcc tgttgatcct tatgtagata
      601 attcctatta cgaaggcgtt gaagcccttg agaagttggt gcagcagaat acggctaatt
      661 tgactttgat gggtattgca cctacatatc ctagtgaaaa gtacggttat atcatccctg
      721 aaagttctaa cgaagtaagt actgttaagg agtttaagga aaaacctgac aaagcaacgg
      781 cagagaaata tttagctcag aatgctgttt ggaatgccgg tgtatttgcg ttcaaacttg
      841 gttacctgtt ggaaaaagct cacgcaatgg tggattttga agattatcgt gatttgtatt
      901 ccaagtatga gactttaacc aagatcagct ttgactatgc tgtggtcgaa aaagaaacga
      961 gcattcaagt gatgcgttat aacggggatt ggaaagatgt cggaacttgg aacatgatgg
     1021 ctgaagttat ggctgataat attaagggga aagctgtaat ggatgatacc tgtgttaata
     1081 ctcagattgt gaatgagttg aatattccta ttcttgctat gggctgcaaa gatatggtaa
     1141 ttgcagcaag tggcgatgga attctgattt ctgataaaga gcgcagtggc tatatgaagc
     1201 cgtatgttga gaaaatcagt acggaagtaa tgtttgcaga aaaatcctgg ggtacatata
     1261 ccgttatgga cgttcaggaa ggctccatga ctgttaaagt ttctatgcag gccggacaga
     1321 agttgagcta ccactaccac aattctcgcg atgaagtttg gactgttgta gcaggacagg
     1381 gtaaagcgat tgtagatggt atgaagcaga ttcttcgtgc gggtgatgtg gtgactattg
     1441 ccgcaggatg taaacataca atcgaagccg tgacaaatct ggatattatc gaagttcaga
     1501 ttggtgaaac tattagcgtc gctgacaagt ataagagttc tttgaactaa aagttcgcga
     1561 ggtagttatt atggagatac taaaatttca acccgtgggt aaagattatc tttggggtgg
     1621 agaacggttt aagacggaat ttggaaaaaa ttgacttaac accattaagc ttatacgtgg
     1681 gaatgcttag ttcatctaga cgataactac gtacggaaat atcaaatatg aaggacagaa
     1741 tggtaaaacc gagttgtggt atgtattgga agctacaccg gggggtgatt tatgcttttg
     1801 agtacgatgt aacagaagaa atgttacgaa atgctgtgat ggacagatca cttgggaaac
     1861 atttatataa agaagaagta cacaagggag atctttttta tgtcagttca tggtattggc
     1921 gctggagtat tagtggctga aatacaggaa agctcaaatg ttacctatcg agtttacgat
     1981 tatgatcgag tagataagaa tgataataag tgagaattgt actttgaaaa agcgattcag
     2041 gtaatgaata tggaagccac tttgaaattg gagaaaagaa ataagctcat tcatttttat
     2101 cctggtggct ctcgtgaaat actgtgtaga tgtaaatact gtgagcccga aagaatccaa
     2161 gtaaagacgg ggttttcgtt ctcggtaaaa gaggaatctt ttcaagttct tttatgtata
     2221 tcgggggacg gacaagttga gtcggcaaat ggtgttatga aactcattag atttgtaaaa
     2281 aagatattgt atgtttttgc caacgaatat tggaagatgt taagtgttga gcgatgcgga
     2341 cttactgaaa attagatggg gatagaggga ggatatctaa tgtcacgaat gagatttatg
     2401 aacacagaaa ttgacaatct tacaatgggt gaagtattgg tagaggttga aaaaatgatt
     2461 caagatagga gatgcggata tgttgtaact ccgaatgtgg atcacattgt tcagttggaa
     2521 acaggaaaag agttgcaaga tgtttataaa aacgcatctt taattttgac ggatggtaaa
     2581 ccattgattt ggatttcaaa gttttataag acaccaataa aagaaaaaat atcgggatcc
     2641 gatatatttt ctttactgtg caagatggct gcagaaaagg gatacacaat gtatttttta
     2701 ggtgcggcag agggcgttgc ggctaaagcg gcagataacc tgatgaaaag atttccggga
     2761 ttaaatgttg taggaacata ctctcctcct tatgggttcg aaaaagacga agatgaaatg
     2821 tttaaaattg attcccaaat taaacgagca aatccagata tgcttattgt gggtttgggt
     2881 tgccctaaac aggaaaaatt tatgttttat aactgtgata gattgggagt acctgtgtct
     2941 tttggtctag gagctagttt ggactttgag gcagggaatg ttaaaagagc accaaaatgg
     3001 atgtcagatt atggatttga gtggctgttt agaattactc aggaccctaa aagaatggca
     3061 aaacgatatt tggttgatga tttaaaaatc tttaaattag cactaaaata tcggaaaaaa
     3121 taaactatat tcaatgagga caatgaagta tgattattac aaaaacacct tttcgcatgt
     3181 ctttttttgg cggcggaacg gatatggaag attattttcg agaaaatggc ggtgcagtgc
     3241 tttccactac ttttgataag tactgttatg tgaatgtgcg tcatttgcct agattttttg
     3301 attacagcac agagttatca tattctcgta ctgagagagt aacaaatatt gaagatatcg
     3361 agcatcctgc aattcgtaat gcgatgaaaa tgctagatat gcatgaaatt cgccttacat
     3421 atgaagcaga tcttcctgca cgttctggtc tgggtacatc tagttctttt gccgtaggta
     3481 tgttgaatgc tttctatgct ttgaagggca agtacgctga caagaagaaa ctcgttgata
     3541 aagcaattta tcttgaaaga gtcctttgta atgaagcagg cggatggcaa gatcagattg
     3601 cagcagctta tggtggcttt aaccgaatca atttcaactc agatggctat gaagtattgc
     3661 ctattattat tacgcctgag cgtaaaaagc agcttaacaa caacttgatg atgttcttta
     3721 ctggattcac cagattttcc tctgatgttc agaaggcaaa taatgtcagc ggcacagagg
     3781 acaaaagagt aagactgaaa aaaatgtatg agcttgtgga tgaagcagaa gcggttctga
     3841 ctgataagaa cagagatcta gatgatttcg gcagactgct tgatctcact tggagattga
     3901 aaaaggctac cggtggtgcg attagcactg gaagtatcga tgaactttat gaaaagggca
     3961 tggctgctgg tgcattgggc ggtaaacttc tcggagcagg tggcggcggt ttcttggttt
     4021 tttatgttca gcctgaaaaa caaaatgctg tgcgttcggc aatgagtaat ttgatgtata
     4081 ttccgtttga atttgaaaac ggtggaacac aggttattca ttacacaccg gaaatgtata
     4141 ttccaaaata aagaaatcag cgaggataag aatatgaaag ttgttattat ggcaggcggt
     4201 aagggaactc gcatctcatc ggttgcaagc gatattccaa agcccatgat taaaattgaa
     4261 aacaaaccgg tattggagca cgaaatcgaa tgtctgagag accagggatt cacagatatt
     4321 attcttaccg ttagccatct tggcgatatc attatgaact attttggcga tggttctgga
     4381 atttcaccag ttactggtca gccctttgac gttaaaattg aatacttttt ggagaaagag
     4441 ccattaggaa atgccggtgc attgtttcgt ttaaaggata atttgaccga agacttcctc
     4501 ctgctgaatg cagatgcgat gtttgacgtt gatttcaacc gatttgtagc atatcaccgt
     4561 gaaaagggtg gcttggtgac attgtttact caccctaaca gtcatccata tgacagcggt
     4621 ctaatcattg cagataagga tggctctgta gacagatggc ttgtaaagga agattcccgt
     4681 ccaacttatt accgtaacag agtaaatgcg ggactccatg tgattagtcc tgctgttctt
     4741 gatgtcgaga taaacacacc taagattgac ttggaccgtc agttattaaa gccgttggca
     4801 ggaaccggaa aaatgttctg ctatgatagt ccagaatacg taaaagacat gggtacccct
     4861 gatcgttatt atgctgtctg tgaagatttt aaggctggaa gagtgaaggg caaaaatctt
     4921 cagaataagc agaaagctgt tttccttgat cgtgatggaa cgattaatca atatgttggc
     4981 tttctgcgtc atattgatga gttcgaacta atcaagggta ctgaagatgc agtgaaaaag
     5041 atcaatgccg ctggatatct ggctattgta ataacgaatc agccagtaat tgcaagaggt
     5101 gaagtttctt ttgaagaatt ggaagagatc cataacaaaa tggagacact tcttggatat
     5161 aagggcgctt atttggatgc agtttattat tgcccgcatc atccacataa gggatatgag
     5221 gaagagcgcc cagagctaaa attcgattgt gaatgcagaa agccaaagcc cggaatgtta
     5281 attcaggcag ctacggactt caacatagat ttgagtcagt cttggatgat tggcgatggc
     5341 gagaatgata ttaaagcggg ccaagccgcc ggctgtaaga ctgcattggt tggcgagagt
     5401 gagtatggac aggatatgac catagcaacc ctgagtgaat ttgcagataa gatctgctaa
     5461 gggatgtttt atcacgaaag gagagtataa acaatgatag acaatagatt acagaaacac
     5521 attgatttgc tgattaaacg ctatcccact cttgaagtaa tcaagcagga tattgttgac
     5581 ggatacctga ttatggagga atgctattta aatgatggca agctgctgat agcaggaaat
     5641 ggcggatctg ctgctgattc cgagcatatt gcgggtgagc tgatgaaaag ctttaagacg
     5701 cctcgccctg ttagtaggga atttgcagat aaattgattg cggttgatcc tgtaaagggt
     5761 tctgaattag ctaagaatct tgaatatggt ttgatggcga ttcctctcgt tgctcatgaa
     5821 gcgctttcca ctgcgtatat taatgatgtt gatggcctag gtgttttcgc acagcagttg
     5881 tttggatttg gaagacctgg cgatgtattc cttggtattt cgacatccgg caatagcaaa
     5941 aatgtgataa atgcaactgt tgttgctaga gctcttggta tgaaagtaat tggcttaact
     6001 ggagccaaag gtggagaact tgcaagggta gcagatgttt gtgttaaagc tcctgaaaca
     6061 gaaacatata tgattcagga gtaccatttg ccaatttacc actgttggtg tctaatgttg
     6121 gaagataaat tttttgggaa tagataaaat atctaagccc atattgaaag gataaaatta
     6181 tgaatgtact atttataaca actcaatttc catatcctct agataatggc ggaaaaattg
     6241 gtgcgtacaa cggtatcagt gttttatcta gagactacga tgtgacggta ttgtctttca
     6301 ccgaagaacc gcagtatgtt gaagaaggtg tcagtttttt tcgagagaaa tttaatagtg
     6361 taaaatttga aaatcctgtg atacatgatg tacacataag aaataatcca atgaaattgg
     6421 tagaagcttt agcaataggg tattgtctac gccaacctta tatagtttca aagttttatg
     6481 attcaaaaat gctgcagtta atagatgaaa aattttctaa caatgcatat tgggatatca
     6541 tttttattga ctatctgaat atgcaaagtt atggtcaata catacgggag aagtataaaa
     6601 cacaatttgg taagtatatc ttaaaagacc acaacataga atatgattta gtcctacagg
     6661 aagctgaatc aagtcgaggt ttaaaaaaga agttgcttca gttagagtat aagcgtactc
     6721 gaaagtatga agaggccgca gtaaataggg ccgattattg ctttagtgtg tgtgatgcaa
     6781 atacggaagt actgaaaaag attaatgaca acagttatac gatgcttcca acttatgaaa
     6841 tgcttacagg ggtgaaaaaa aatatagaga agaacacgat cctatatttg ggtaaccttt
     6901 cttggaaatc caatatgcaa ggattagttt ggtttttaga gtcaatttat ccaaatattc
     6961 aacaaagaat tccggatgta aaaatcacaa ttgtcggtag cgggccagtt ggaacagatt
     7021 ttagtaaata ttcaggcgtg gattatcgtg ggtatgtaaa agatatttca cacatttatg
     7081 acgatcaagc ggtgctaatt gtacctttgt tagaaggtag tggtataaga ataaaaatat
     7141 tagaagcgtt caataatgaa attgcagttg tatctacatt agttgggtgt gatacgattg
     7201 gcgcaaaaga tggaagtgag ttatttatag cagataacga aaaagatttt gcaacggctg
     7261 tagttagact gcttgaagac atgaatttga atgaaaaagt aagaaagaat gcaaaattat
     7321 tcttaaaaga aaaatactcc ttaacggcta gacaagatga gtttagtgcg ataattaaca
     7381 gctaatatat atataagctt atctgatgat gatttatagg aggaaatata tggagatggc
     7441 tgagagaaag gaaaagaaac ttggaaataa gaatatcata ttattttaga aaactcctgt
     7501 tattttatgt ttgtgtgatt gttgcggcta tgagttacca ccttacaggc aattccaatg
     7561 tagcgtcatt aattgctgta gtaggagtag tttatgctat tgtaaaggcg tgtaataacg
     7621 agtatctggc tatatgggtt atgtttgtat taggttctga tatatggggg atagtgccat
     7681 atattaacat tggtagtggt gtgttgcatt ggtcagattt taatgttata gtagcgatat
     7741 ttctctgctt gagattcatc aaagttaaag ctaggagtag atacgggatt gtctatagtg
     7801 taatgcttat atttattttg attgcatgtg tgcaatcgca ttcaatctat ggacaagatg
     7861 tacatacaac gctcttaaca gctagagaat tgctagttgt aatatccttt tggccgatag
     7921 taaaaatagt atattcagaa agaataacta aaaatgaatt cattgattta gtgtgtaaat
     7981 tcacaagatg gacaatgtgt atatattggt ttgaatggat tcttgttaat atgggattca
     8041 atataacctt tttacagaca ggtgcacgct ggggtactag aatctatata gatccgatat
     8101 ttatcatttt atatttcttt ataaatctat atgcggttat atataaaaga gagggctata
     8161 atagtacaaa taaaagtata tggaattgca tttttagttt actgacattt gttataatct
     8221 cgcaaggcag aagtggtata ttatatgtag taataacctc ggttattatg cttttaattt
     8281 cgcttaaacc atccaagatt attagatttg gtatattagg aattgcctta attgcatgtc
     8341 tagtgtctgt gacccaaatt agagaggtcg ttatatcttc gtttgtagag tctcagagca
     8401 gtgacacagg taatatcgca tatagaattg ctgaacaaga ttattttgaa aataaattag
     8461 aaggtcatga actatttgga gttggtatac caaacaatca tgatgccaga gctgtagaat
     8521 attcaggcaa aacaatagat tttgagtcta aaaaatataa tccctattat cttgaggact
     8581 taggcgcttt tgaaattcgt tatagatttg gtataattgc atatgcgtta tatatagttg
     8641 ctttagctat aagtattata gatgccggat ttcgaagaga caaatcggtc gccgcatttg
     8701 cgggtatggc attcatggtg tttatggcgc ttaattttaa tttgctagcg tatattacag
     8761 caagaccgtt cggatttatg atttctttaa ttttgataga aatatcggca ttcaatgatg
     8821 aattcatata tattgaatag gtaagggaga gcacagatga atttagatcg attaaagaaa
     8881 atagcaaata tgatttcacc aatcataaaa gtgataaatg cgccgttagc gtattttagt
     8941 gggaaatcag attatatgga gaagggaata ttgtggccta ctgtaacaac ctactccaat
     9001 aacagtagaa aagtgattca atatacaact ttacctaaaa acgtagacaa catggctcaa
     9061 atgcaatggg ctggggaaag ttttgaggac atatgtatag ttcttcaagg tccagtatta
     9121 gaagacggtg attttacact aaataccgtt aaaatgtata ataaatacta tcctaaagta
     9181 aaacttgtag tgtctacttg gataggatta tctgaagaga cagaatgcaa gttgcgtgaa
     9241 gctggtgcag atgttgtatt atgcaagaaa cctgaaaaaa caggtgcgta caatattaat
     9301 ctacaactta cttctgcata cgaaggcgta aaaatggcgc aaaaactggg gtgtagttat
     9361 gcgctgaaaa cgcgaactga tcaaagacta tatgcaaatg atgtactaaa gtatttccaa
     9421 acattattga atatttttcc atcttcggac tgtaaagtag tgaatcagcg attaatattt
     9481 acgacttatg gaaaatcatt tagatatctt ccgtttaatt tatgtgattt tttggtatac
     9541 ggagaaacta gtgaattatt aaaattatat ggtattcaaa gagataaccg agcatcagac
     9601 tttgggaaaa ttatttatac tgaggaaaaa gagtttaata aaaagatatt tacactacta
     9661 gaaggaaaac aaggtgtatc tccatatgat gtttttccag attttgatgc actatataaa
     9721 aaatatatgt ttgcagaata ctatattgtg tataattatt tcctggaaaa tattgcaagt
     9781 aatgaatctt atggtgactt aatggatgcc tattatagtt atttaaagaa atatgcagtt
     9841 gttgctgatt cagaaaaatt gttactatat tggccgaaat atgttcaata taatgttaag
     9901 ttcgaaaatg aacttgattt ctatgcgaag ttggattcaa aaaaatggat ggatatttat
     9961 gctaattata caccttttag tcagtgatta tatggaaagg atgaggtgaa taatgaaaat
    10021 cggaatagtg actattgtta atggggcaag ttttggtaac agattacaaa attatgcgac
    10081 tcaaaaagtg ttggaaaata acggctatac agttgagaca attaataatt gttttgacaa
    10141 agaaggtacc ctatccattg ttatcaaaaa cgctatttat acattattta agtttatacc
    10201 gcatttcaaa aagtcaaaaa gtatctggaa aagaatgtat acattttatc aatgggacaa
    10261 gcagtatata gattattctc atcgtaaaat tgatgcatct aaaaaaaaca gctttgtgag
    10321 tataaagaat gattatgact atttcttggt tggtagcgat caaatttgga atccaaaata
    10381 tgcagccaat aagggatttg aacttttatc atttgttcca aatgaaaaaa gagtttcatt
    10441 atcaaccagt ttgggagttc catctatacc aagtgattta gaaaaattat atgtgacaga
    10501 gtggaataag tttaaatcaa tatcggtacg tgaaaatcaa gcggcaaaaa taattgagga
    10561 cttgacaggc acaactgcgg ctgttcacat tgacccaact ttaatgttgg agcgttcgga
    10621 atgggaagcc tttcaaaaaa aaccagacaa tattgatgaa aattacatct tgtcgttttt
    10681 cttaactaat ccgcctaagg atactgttga tgtattagat aaggtcgcta aagatttgaa
    10741 aataggtgtt ctcaccttag atgcgtcttg ggaatatttt caagatatta ctccgaatca
    10801 ttttgtttat ttgatttcac atgcaagaat gatttataca gattcattcc actgcacagt
    10861 tttttcattg ctatttggaa aaccggttgt ggtatttgac cgttttgata gtgaaaaaat
    10921 gaattctagg ttaagtacat tgttaaataa agtaaataaa gcacattttt gggtaggaaa
    10981 aaaagctgat gaagcaatga ttcgtgcaat agcatctgaa cctgaatatg acatagataa
    11041 gctgatggta tcagagaggg aatgtgtaaa agcttatctt aaagagtatt tacagtaaaa
    11101 ttttgaaatg ggatgaaaat atggagcatt taagtgattg ttttggtaaa atgaagattg
    11161 ttacgacttc atgcgcaaga gaatatttta aggatgattt ggataaatca gagtattcat
    11221 gttttgttga ttctcgagca gctatgtttt tcgcaatggg acaagcttta gctaaaagaa
    11281 ggaatgtagt tgtaatgctt gctgatgaat tgacatcgtg ttatacggca ctaacagaat
    11341 gtgcatttca acaagttgat ttaattgtgt ttgcttttca gacagtcaga catgaaattg
    11401 atttagcatc tttgaagaat tgtggttgca atctaattaa ctgtagcgcc caagacgata
    11461 ttgatagagt tgtgtccaac actaaggggt taaatcagac aacagtaatt aaaatcaata
    11521 tgaattgtga gtcggataaa gatcatatat gtgagtatat agacctgcca gacatacctg
    11581 tttttttcca cggtagctta tactcacctg caagtaaaaa gaaatatgag cagtatggac
    11641 aggtatcgaa gtatttaggc tatctcgtgg ggggaaaaga tgcaatactc ataatttctc
    11701 acaatgagtt tttaaacgat atcagtgtat ggattaatta ttattctttt ggagaaattc
    11761 aacccaaaat tattatattt gacagtccga atgactatca atataaatgg ctgtgtgact
    11821 tagaatgcag tgtagctcat tcaaccaacc gatttttgtc tgaggattta gccttattgc
    11881 aaaataacgg aatttccatt gtgttaagag gtgataatta tgatatcgtc agttaaaaat
    11941 gttcaaattt tagtatcttt attaaaacag cataatatta aagatatagt tatctcgcca
    12001 ggtagtagag attttcaatt ggtgcatagt gtagaaacag acagtttttt
//
LOCUS       DACCCM010000082        11885 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCCM010000082 DACCCM010000000
VERSION     DACCCM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.086"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(451..1761)
                     /locus_tag="IVK78_002304"
     CDS             complement(451..1761)
                     /locus_tag="IVK78_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6375744.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IVK78_002305"
     CDS             complement(1773..3191)
                     /locus_tag="IVK78_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375745.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IVK78_002306"
     CDS             complement(3431..4999)
                     /locus_tag="IVK78_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP6375746.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IVK78_002307"
     CDS             complement(5003..5413)
                     /locus_tag="IVK78_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6375747.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IVK78_002308"
     CDS             complement(5410..6333)
                     /locus_tag="IVK78_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP6375748.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IVK78_002309"
     CDS             complement(6330..7208)
                     /locus_tag="IVK78_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6375749.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IVK78_002310"
     CDS             complement(7195..8532)
                     /locus_tag="IVK78_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6375750.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IVK78_002311"
     CDS             complement(8535..9638)
                     /locus_tag="IVK78_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6375751.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IVK78_002312"
     CDS             complement(9712..11220)
                     /locus_tag="IVK78_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6375752.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFFNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IVK78_002313"
     CDS             complement(11186..11878)
                     /locus_tag="IVK78_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6375753.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
BASE COUNT         3510 a         2555 c         1932 g         3888 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgc agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattaaagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttc
//
LOCUS       DACCCM010000083        11689 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCCM010000083 DACCCM010000000
VERSION     DACCCM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.087"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1589
                     /locus_tag="IVK78_002314"
     CDS             <1..1589
                     /locus_tag="IVK78_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAP6375754.1"
                     /translation="RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAK
                     DAFANGIQLVLLTADRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYAT
                     RKINEAILELNHGVKGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQ
                     KLLSKKRIMVLCGQQEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERM
                     SVNAFIDYCPDLVITIGKHVWSFVKYTLRDKKLKFEHWRISEDSKIVDPFNKLTSVFQ
                     CSAEFFMETVTAGVNSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSAL
                     VHSSILNAARLYDFVSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDL
                     SFFYAMNSLCGDIPDNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKD
                     WALENNIKYITADSKESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVI
                     TPKMYFRKALDIIKKHLHIK"
     gene            1615..2451
                     /locus_tag="IVK78_002315"
     CDS             1615..2451
                     /locus_tag="IVK78_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6375755.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            2456..3889
                     /locus_tag="IVK78_002316"
     CDS             2456..3889
                     /locus_tag="IVK78_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6375756.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
     gene            3906..5567
                     /locus_tag="IVK78_002317"
     CDS             3906..5567
                     /locus_tag="IVK78_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP6375757.1"
                     /translation="MERRHGEMRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYL
                     YDQYKDVCSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCD
                     EPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDD
                     GKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNW
                     FDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVE
                     YVRPRIFEYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKR
                     YSVSGDNIQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALL
                     SVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELA
                     KLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDL
                     KKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
     gene            5655..6053
                     /locus_tag="IVK78_002318"
     CDS             5655..6053
                     /locus_tag="IVK78_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP6375758.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            6078..6794
                     /locus_tag="IVK78_002319"
     CDS             6078..6794
                     /locus_tag="IVK78_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375759.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            8026..8605
                     /locus_tag="IVK78_002320"
                     /pseudo
     CDS             8026..8605
                     /locus_tag="IVK78_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            8665..10317
                     /locus_tag="IVK78_002321"
     CDS             8665..10317
                     /locus_tag="IVK78_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6375760.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLIGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            10661..11026
                     /locus_tag="IVK78_002322"
     CDS             10661..11026
                     /locus_tag="IVK78_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP6375761.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            11114..11557
                     /locus_tag="IVK78_002323"
     CDS             11114..11557
                     /locus_tag="IVK78_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375762.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
BASE COUNT         3602 a         1665 c         2437 g         3985 t
ORIGIN      
        1 ttagaaccta tacggttgta gatgagcgca gtgctgcctt ttttgctaat ggactttcta
       61 tggcgattga taagcccgtg tgtgtatgtt gtacttcttc aactgcctct tcaaattata
      121 tggcggcagc taaagatgct tttgctaatg gcatccaact tgtacttctt actgctgata
      181 gagattaccg ccgcttatat cagatggaag atcagatgat tgatcaggta gatatgtatg
      241 gggcttatgt gcgttctaac gttgatattc ctattgttaa ggattctgat gatgaatggt
      301 atgctaccag aaaaataaat gaagcaatat tggagttaaa tcatggcgtt aagggtcccg
      361 tgcagattaa ttatcaatta atggatttaa gtgcgccgtt tgttgagacc atgccagagt
      421 atcgtaaaat tacaagatat caagaggatg atttactgtc atcagtagag atttttaaac
      481 agaaactatt atcaaaaaag agaataatgg ttttatgtgg acagcaggaa gaaaataatt
      541 taagtgcctt attggaagag ttctttatta aatataatac aattataatc aaggataact
      601 attctaatat acacggtgaa cactttatta agactgttct agtaacagaa agaatgagtg
      661 taaatgcatt tattgattat tgcccagatt tggtaataac tattggtaaa catgtatggt
      721 cttttgtgaa atatactctt agagataaaa agcttaaatt tgagcattgg cggatatctg
      781 aggatagtaa gattgttgat ccgttcaaca agctaacatc tgtctttcag tgctcagcgg
      841 aattttttat ggagactgtc actgctggtg tgaatagcaa taataatctt gagtattata
      901 atctatggaa gacaagatta tcaaaagttt gctatccaga gttagtctat agcaattttt
      961 atgcaatttc agaactagta aaaaaaattc cagatagtgc attagttcat tctagtattt
     1021 taaatgcggc tagattgtat gactttgtat ctgatgataa agcattactc agttttgcta
     1081 atttaggggc tgatggtatt gatggttgtt taccaacatt tttaggtcag gcagaagcag
     1141 ataaaactcg acctgcgtat ttaatttcag gagacttgag tttcttttat gctatgaata
     1201 gtttatgtgg tgacattcca gataatgcac atattttcct tattaacaat cattctggaa
     1261 gtgaattcca tacaaatttt ggtaagtttt acaacggatc attaagtgtt gataagcata
     1321 tagctgcagg acatatttca aacgcaaagg attgggcatt agaaaataat ataaaataca
     1381 ttactgcgga ttctaaggaa agtctcaaat cggggttgga tgtatttatt aatacatttg
     1441 acagtcctgt tttatttgag gtctttacag atccggatac ggatacgtct gttctggaat
     1501 ctttttatag tataaataag gtaatcacac caaaaatgta ttttagaaag gcgttagata
     1561 ttataaaaaa gcatttacac attaagtaga tgtttattgc gaggtgaaac aaatatggca
     1621 ataaaatctt atattaaacg gttattgctt tatatccgtc atggagatcc caaaataaat
     1681 ttagatgtga gttatttatc tgaaggtgca atgcttgctg gtaagactgt gataattacg
     1741 ggtggtgcaa aaggacttgg ttttgcaatt gcaaaatcat ttatagctca aggcgcaagc
     1801 gttattatta caggaagaga catggattcg ttgaataatg ctaaatgtca gcttggagat
     1861 atgtgttcta tcagggcgtt tgatataact gatgctaaaa tgtatcgaac gttttttgaa
     1921 gatgcatacg agacctttgg acgtattgat atactgctta ataatgctgg gatctcgtta
     1981 cacgaagatg gatttatgag tgttactgag aaaacttggg ataagcaaat ggatacaaat
     2041 ttgaaaggtg tttatttctt tacacaggaa tttattaagt ttgttaaaga aaagaatcaa
     2101 atgcaagcaa atattattat gatgtcctca gaaagaggct tgtgtggtga tagtattccg
     2161 tatggattat caaaggctgc aattaactct ttagttgtag gcttgtctaa gaaatatatt
     2221 tctgagggaa ttagggttaa tggtattgct cctggagtaa ctgcaaccgc tatgactggt
     2281 atttctgaaa agggtaattt gtataatcct gcaacgatcg gttatagata ttttctccca
     2341 gaagaagttg ctcaagttgc tttgtttttg gcaagtagtc tttcaaactg tatcagtggt
     2401 gaaatcatag cctgtgacca ggggcaatat ttaagtcgaa attgggggta ataacttggg
     2461 aatattatta aaaaaatata atagccttag tgagcctgca aaagcatcta tttggtttac
     2521 actttgtaat gtgttgcaaa aaggaattct atttttgact acacctattt ttacaagaat
     2581 gctttcaact gcagaatatg gtaagtatac gatttttata tcgtggtatt ctattatagc
     2641 aattttcaca acgttgaatt tatctgcagg tgtctataat aaaggactaa ttaaatacga
     2701 gaataaatat gcattaacct cgtcattttt aggattatcg tcatttattt ccctttgtgc
     2761 attgatgcta taccttccat ttagaacggt tataaatggg ctactggatt tatcgacgga
     2821 gtttgtcata ttgattctta ttgaatcaat atttgagccg gcttttttgc tttggtcagt
     2881 taaggaacga tttgattaca agtatcaaaa actagtactc atcacagttc ttatggctgt
     2941 tttaaatcca ggtctcggta ttatattaat cttatcattt ggggagtatg ctatttggag
     3001 agtacgagct tatgtcgtgg taaatgttgt agtggggatg tttattggca taggaatact
     3061 ggctaagggt aagaaattat ttgataaaga gtactggaca tatgcgctca gatttaattt
     3121 gccattaata ccacattatt tatcaatgac attgctgaat caggttgatc gccttatgat
     3181 aaatagtatg gttggagcta gcgaggctgc gatttatagt atcgcttaca ctatatctag
     3241 tatgatgaat atcataacaa cagctataaa cggttctttt attcctttta catacaaagc
     3301 aattaaagat ggcgtgtatg aaaaaattaa atccgttgct aatcaactat tagtgttgat
     3361 ttttgtcttg tgttcgttta ctatgctgtt cgggccagaa ttgatttggg ttgtcggagg
     3421 agaaaaatat atggatgctg tttggattat tcctccagtt gcggcatcag tatttttcat
     3481 gtttttatat ccgctttttg gcaatattga attttactat gaaaagacaa actacgtaat
     3541 gtttgcatct tgtgtgggct ctatcgcaaa aattatttca aactatgtta gtattccccg
     3601 ttttggatat atttcggctg gttacacgac attgatttgc tatatgttgt tttcctttgc
     3661 ccactacatg ttttataaaa aaattcttag agaaaatggt ataaaagaag atatgtataa
     3721 tattaaaaat attatatttc tttctgtctt aatgattgtg ttaactattg gtatgacaat
     3781 attatacttt tctacaattc taagatatag tattattatt ggattagtta ttgtggcttt
     3841 tcttaaaagg acgcaaataa tgaaaatcct taaaaatata aaaaattaac aagcaaatct
     3901 tgggaatgga acggaggcac ggtgaaatga gattattgat tccatctgct aagattgtac
     3961 cggaagaact tcaccatctt ggaaaacttc ctgcaattat ctatccgatt aatcaaaaaa
     4021 ttgtatttga ttatttatat gatcaatata aagatgtatg ttctgctatc gatattgcct
     4081 gttacgaaaa gatggataag gtagcaagac gattagataa gtatattaaa tctaaaacag
     4141 taaatattat tcagcttaaa gagttagggg atcttggaag aactatatat gattctctaa
     4201 taggatgtga cgagcctgtt attattaatt ttgctgatac cattatcaat gataatatat
     4261 attccttgga atgtgacagc tttttctatg ctgaagacta ttatagtaat acttggacat
     4321 tctttgaaga aaaagacgga gatattattt ctgttttaga taagaacgag ttaaaagaag
     4381 atgatggaaa gaagcacaaa ctgttttctg gtgtatttca aattatggat gcccagtatt
     4441 ttagagagtg tttgcgtaaa gctttgatgt caaatgtagt aaatgtgaat agtttttatc
     4501 aagctttaca agagtatagc aaacgatatg aatttctctc tattaaaaca aataattggt
     4561 ttgacattgg acatgcagat aaatattata attccaaatt ggaagttaag gcaagagaat
     4621 ttaaccacat ttcaattgat aaagatcgta gcatcctgag aaaaattagt gaagatgttg
     4681 aaaaatttat cggagaaatt aagtggtatc ttaagttgcc agcacaggta gaatatgtga
     4741 gacctagaat attcgaatat tctacttcgt atattaatcc atatgtaagt atggaatact
     4801 attcgtatca tactgttcac gagttatttc tctatagtga cttaacaaaa aaacaatgga
     4861 tagatatctt taatagaata cgatttgtat gctcagactt taagagatac tctgtttcag
     4921 gggataatat tcaaaagtca ttgaaagata tgtatctcga taaaacattt cagagattca
     4981 ataagctcag aaaggacccc cggttcacag aattcttttc ttctgatatt caaataaatg
     5041 gagtcagata taaatctttg gaccagatag aagctttatt gagcgtaagt gttccaagag
     5101 aattgtttga cattacgcaa tttaatatta ttcatggtga cttatgcttt gccaatatta
     5161 tggtagataa cacattttcg ttcattaaag tcatagatcc gcgaggtaag tttggagatt
     5221 ttgatattta tggtgattat agatatgaac tggcaaaatt gtttcatagt gttgatggaa
     5281 aatatgactt tataattaag gacttgttta caataaaata tgatccaaag aaggcaatca
     5341 tagactatat tgttcaagat cgtaaacgag attatgattt atatgaagta ttttactctg
     5401 tcttcaaaga tgaaataggt tctgatttga aaaaaattga acttattgag gctttgctgt
     5461 ttttaagtat gattcctttg catggagaaa gcttaaacca tcagatggct atgttagcaa
     5521 ctggtttgga aattttagga cgagttgtcc cggatattta ttgttaatgc tttgagctat
     5581 tgtttttcta tttgatcagt gtaaagccgg gcatataaat tgcctggcta aaataaagga
     5641 gatgtcttag ttatatgttt aaagattacg catttgtttt tgatattgat ggaactttgt
     5701 gccccatcaa aaagaaagat gagaaatatg aagatttagt tccgtatgct aatatggtgg
     5761 agaagttgcg atactataag gagaacggag caaagattac actgttcaca tctcgcaata
     5821 tgaattctta tcatggtaat ttaggtgtga tcaatgcaaa tactgcaaaa atcttgttgg
     5881 cttggctaga taagtgggag attccgtatg atgaaattct ttatggaaag ccttggccag
     5941 gccataaagg gttttatgtt gatgatcgca cagttcgtcc ggatgaattt ttatcgtgta
     6001 ccctggatca gctcgatgag atttgcaaaa aatccaaatt gaaatctgag taaaggagac
     6061 tttataaagt gaataagatg acggttatta ttacaatggc tggtcttggc agtcgctttc
     6121 gtaaagttgg ctacactgtg cccaaattta tgattgaagc aaaaggtaaa accttatttg
     6181 attggtcctt agacagcttg cttgattata attcacatgt agaaaagtat gtttttgttg
     6241 tgcgtaagga agatgatgct actgttttta tcaaagaaca ttgcagtctg tatggaattg
     6301 aaaatgtcga ggtagttgaa attgactata tgactgatgg acaagccact acatgtatgc
     6361 ttgcaattcc ttattgcgca cctgataatg caattatggt ttataacata gatacatatg
     6421 ttgagccagg tgagatgaaa tacgttgata tttctggaga aggacatatt ccgtgtttcc
     6481 atgcggacgg tgatcattgg agttttgcca agttgaacga atatggtgat gtagtagaag
     6541 taagagaaaa agttagaatt tctaacaatt gtactttagg tgcatattat ttttcttctg
     6601 caaaattata tgcatcactc tacgaagaat attatgcaga tgaaaggcat ctggaaaaga
     6661 atgagaaata tattgcgccg ctatataatt atatgataga gaagggcatg aaggtaacca
     6721 ttagtatagt agataaatca aaagttcacg tactcggtac tccagaagag ctcgatgtgt
     6781 tcttaaatga atagggaaaa tataatcata gaaaatgtta attataaagc tgcgtgtgtg
     6841 ggggagtact attcgataga tattctaaaa atactaatgg ctattttagt tgttgttcgt
     6901 catttaggtc aaaactattt agctccagat taagcattta gtattggtat tgttgcagga
     6961 ctgagtacgg ttgccgttcc tacttttttc atcatcagtt cctttttgtt gtgtagtggt
     7021 gaagttacat ccgctaaatt atctaaacaa atctacagag tacttaaaat ttacattatt
     7081 tggtctatat tatatattcc attagtatta aagaattggg tacagtcaga ttttagctgg
     7141 aaaacagatg tattattgta tatcagagat tttctgttta ttggtagtta ctatcacctg
     7201 tggttcttgc ctgcactggc ttttgctcta tggttagtac gcaaaagcca ggtttctaaa
     7261 ataaagtttc tcagtggttt gttgctatgc gtagtaggtt tagcatttga atcttattgg
     7321 tttatattat ctgggaatga ttccatcgag tatattagat attgttataa aagcgtgttt
     7381 gtaacaactc gtaatggatt atttataggt gtaccatgcg tcattattgg gcaacactta
     7441 gccttccaca aaaacactat acttaataag gtctttaaat accgtaattt tatcttatgt
     7501 gctgttttag ctataggatg cttattagtt ggtgaaagtt tcatcataaa tcaaatagct
     7561 ttgcgagaag tcaataattt gttaatattt tccattccat tagcactggt agttgtatca
     7621 gtatcactga gcctaaagat agtacattta taaaaataac agtacatctg cgaagaatta
     7681 gtacgttatt attttgttta catccgtata taatagctgc tttaaatttt attttcccaa
     7741 aattggggat tacaaattca ttaatagaaa tgacttgtgg aatgataata attttaattt
     7801 ctacagtttt tgtgtatgcg tgtagtgtga aatttacctt gctgaaaaaa ttatattaga
     7861 ttagtaactg gtacatgtaa atttgttgaa attatagcag catagagata ggaattctgt
     7921 tgagttttat tttcgctggc cgaggttatt catgttagag tgggtacctc cttttacgaa
     7981 accttcttga tggtactcat ttagcgcatg atgaaaggag agtttatgaa gaaaatcata
     8041 tcttctattt tggttaatgt gttatctgca ttgtatcaaa tatttgggtt tcgattttaa
     8101 ttgcagtttt ggctatgttt ttatacttgt ttgcgatggc tgaggagaaa tcaggattag
     8161 gtttgaaaaa atcttttctt gtctggattc ggtatttcag aaaatcaagc tcatttagaa
     8221 aaatgttttt gcttgtgttt tttacaaata tgattctttt tagaacactg atggatagat
     8281 atgtttggct aaatcctttg tctaatgtgt ttggtagttg gtggatttac tctgtgaatg
     8341 aaacgacagg ggagattaga ttaactacag aatgttttga aaatgttatt ctgtttattc
     8401 catttataat tctagtaatg tggtataagg cttcagaaaa agattttgga aaaatttatc
     8461 agagattata aaatttgttt ttttatgttc gtgtcttata gagtttttgc agttattttt
     8521 acacatagag acattccaat tgtcagattt attttataat actttgggtg gcggcattag
     8581 tggggttata tattatttga gctgatatat tcatcctaac cttaaaaata aatgagcaga
     8641 attttaaaag attggagaga ttctatggat cataatgcaa attattatca ttggcttaca
     8701 aatgccaatg cagaaattgt agaggaactg cggtcatatt ccgagaagga cattgaggac
     8761 tccttctata aaaatctgtc ctttggtacc ggtggtctgc gtggcaccat tggtgcaggc
     8821 actaatcgta tgaacgtaca caccgtaggt aaggcaagcc agggactgtc agactatctg
     8881 aataagacct atgaaacccc ttctgtcgtc gtgggctatg attcccgaat caagtccgat
     8941 gtatttgcaa aggttgccgc ggacatcttt gctgcaaacg gtattcaggt gcatctgtgg
     9001 ccggagctgc tgcctgtgcc taccgtatcc tttgctatcc gttatctgaa ggcttctgcc
     9061 ggtgttatga tcaccgcatc ccataatcct tccaagtata acggatataa ggtctatggc
     9121 gcaggcggct gccagatcac caccgaggct gccgctgaga ttctggctga aattgaaaag
     9181 ctggatattt ttgcagatgt gaaaatgggc ggcgacgctt ccagaatcgc ttctatccag
     9241 ccagaggttc tgactgcttt cattgaagaa gtaaagggcc agtcggttct gtttggcgaa
     9301 gaagtggaca agaatgttgc tatcgtgtat tctcctttga atggtaccgg tctggtgcct
     9361 gttacccgta ctctggcaga aatgggctac agcaatgtga ccgttgtcga agagcagcgt
     9421 ctgccagacg gtaatttccc gacctgtcct tatccgaatc cggaaattaa tgaagctatg
     9481 tctctgggca tggagtatgc caagaagtat aatgctgatc tgctgctagc taccgaccct
     9541 gactgcgacc gtgtgggtat cgccgtgaag aatgcagaag gggattatgt cctgctgtcc
     9601 ggtaacgaaa ctggtctgtt gcttctggac tacatatgct ctcagagaat taagcacggc
     9661 aagatgccca ctgaccctgt gatggtcaag accatcgtga ctatggatct gggcgagcgg
     9721 atcgcttcca actatggcgt gcgtactgtc gatgtgttga tcggcttcaa gttcatcggc
     9781 gaacagattg gtatgctgga aaaggcaggc aaggtggaca gctatatttt cggctttgag
     9841 gaatcctacg gctatctatc cggcagctat gtccgtgaca aggatggtgt caacgccgct
     9901 tatctgatct gcgagatgtt ctcttactac aagactcagg gtatcagtct gtttgataag
     9961 ctgaacgaga tttatgctaa gtatggctac tgtttgaaca ccctgcattc ctttgagttt
    10021 gagggtgcgg caggttttac caagatgcag aaaattatgg ttgaattcca taaaggtctt
    10081 gatatgattg gaggtaagaa ggtcattgaa accttggatt acagcacggg actgaatggc
    10141 ctgccgaagt ctgatgttct caaatatctg ctggagggca actgctctgt agtggttcgg
    10201 ccttccggca ctgagccgaa gctgaagacc tatatcagcg ttagcgcaga aagcaaggaa
    10261 gctgccgaag taatggaagc tgttatggct gctgaactgg ctgaaatgtt taagtaatac
    10321 tatgaaaaag atctgagtgt caatgtccga agtagcaaca tctatgttta ataaaaattt
    10381 cctgctttac ggttctgttt acataattga ggtaaagggg ccgactgtgt ggatcgaaaa
    10441 ggcaaagaag tgttaacaga gacacagcag cctgatgaag caccgatcaa agcagtaagg
    10501 gagaaataag agatattaga gttttaataa tgtattcagc aatagagatg aaaggagtag
    10561 aatatgaata aaactattga aataataaaa tgagggggaa agaaataaac gtgatattat
    10621 tagttttgag aaggcgtttt caatattcgc tattgtattg atgtactatt ttcagcaatc
    10681 gtatttgcca gcaatactga acaaagcaat atctattggt gatacaggag tgcatatatt
    10741 cttcttttgc tcaggttttg ggctatatct gtcatattta aaaaagccta ttacattggg
    10801 tgaattttta cagaaacgat ttatcaagat ttatattccg tatattatcg taattacggt
    10861 atcagtgtta ctaccttata tgacacaaga tttaaatgct gcaaaagtat atttgtctca
    10921 tgttttgtta tttaaaattt ttctaccagt atatgaggag tcactgggtc cctttggttt
    10981 atttcgacaa ttattcagtt ctatttctta ttcataccaa ttgtaaaact atagagaaaa
    11041 ataggaatga aatgattgct tgttgcaagc tgagttatta gtatctgggc tattatgatg
    11101 tttcatacag gatatggaag catacgagtt cttgggagct gctttgcgca atatatttgg
    11161 gaatttactt tcggcatgtt tggttgcaga gtatttagtg aaaagaaaaa tagtaagatt
    11221 taataatata ttcgtgatta tggcgtccat tgttggaatt ggttttgctg gtatatcgaa
    11281 aattattggt ggactgatgg agtctattaa tgatattttt gcattcttcg gttacggtgc
    11341 aattgcaatt ttaatgtatc aagttattcc atttagaaag ataatgccta ggatttcaag
    11401 atactcatat gatatatatc tactccatat tcttgtattt agtacgattc atcaaattat
    11461 ccctaacttg gttttggcgg catttgtttc cattcctata acgttggtgt tgtcatatta
    11521 ttatagtatt atgttatata gacttaaatt aaaatgatag cataattgtc gcctctaccg
    11581 ggcggttgcc atcctgatta acagcctcca cgcggcggat ggtgggcact taggtagttc
    11641 gtaacctagt ggtagatggt tggtaaaaag gagataaaac taaatgatt
//
LOCUS       DACCCM010000084        11510 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCCM010000084 DACCCM010000000
VERSION     DACCCM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.088"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(79..2154)
                     /locus_tag="IVK78_002324"
     CDS             complement(79..2154)
                     /locus_tag="IVK78_002324"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP6375763.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IVK78_002325"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IVK78_002325"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP6375764.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IVK78_002326"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IVK78_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP6375765.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /locus_tag="IVK78_002327"
     CDS             complement(4412..5311)
                     /locus_tag="IVK78_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP6375766.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5727)
                     /locus_tag="IVK78_002328"
     CDS             complement(5326..5727)
                     /locus_tag="IVK78_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP6375767.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IVK78_002329"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IVK78_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP6375768.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IVK78_002330"
     CDS             complement(6198..8390)
                     /locus_tag="IVK78_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP6375769.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IVK78_002331"
     CDS             complement(8414..9385)
                     /locus_tag="IVK78_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP6375770.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IVK78_002332"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IVK78_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6375771.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3106 a         2419 c         2105 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc gtgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
//
LOCUS       DACCCM010000085        11275 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCCM010000085 DACCCM010000000
VERSION     DACCCM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.089"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(51..560)
                     /locus_tag="IVK78_002333"
     CDS             complement(51..560)
                     /locus_tag="IVK78_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375772.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(550..1275)
                     /locus_tag="IVK78_002334"
     CDS             complement(550..1275)
                     /locus_tag="IVK78_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375773.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(1498..3618)
                     /locus_tag="IVK78_002335"
     CDS             complement(1498..3618)
                     /locus_tag="IVK78_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6375774.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3848..5026
                     /locus_tag="IVK78_002336"
     CDS             3848..5026
                     /locus_tag="IVK78_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP6375775.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5042..5800
                     /gene="aroD"
                     /locus_tag="IVK78_002337"
     CDS             5042..5800
                     /gene="aroD"
                     /locus_tag="IVK78_002337"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP6375776.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5823..6308
                     /gene="ybaK"
                     /locus_tag="IVK78_002338"
     CDS             5823..6308
                     /gene="ybaK"
                     /locus_tag="IVK78_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP6375777.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6379..7632)
                     /locus_tag="IVK78_002339"
     CDS             complement(6379..7632)
                     /locus_tag="IVK78_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6375778.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIHEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7749..9098
                     /gene="gdhA"
                     /locus_tag="IVK78_002340"
     CDS             7749..9098
                     /gene="gdhA"
                     /locus_tag="IVK78_002340"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP6375779.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9210..10556
                     /locus_tag="IVK78_002341"
     CDS             9210..10556
                     /locus_tag="IVK78_002341"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP6375780.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10864..11250)
                     /locus_tag="IVK78_002342"
     CDS             complement(10864..11250)
                     /locus_tag="IVK78_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6375781.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3666 a         2020 c         2293 g         3296 t
ORIGIN      
        1 atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg
       61 ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag
      121 ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc
      181 aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag
      241 gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta
      301 agcaaggaag gagagatatc aagtttttct tgataggcca aacgatcttg tttgatttct
      361 ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa
      421 tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc
      481 tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga
      541 atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa
      601 agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc
      661 aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt
      721 ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg
      781 cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga
      841 ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag
      901 ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat
      961 caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa
     1021 tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg
     1081 aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc
     1141 taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct
     1201 tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata
     1261 gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag
     1321 tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag
     1381 aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta
     1441 ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttatca
     1501 ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc
     1561 aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt
     1621 ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg
     1681 gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac
     1741 agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt
     1801 aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata
     1861 ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa
     1921 tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt
     1981 aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg
     2041 gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga
     2101 acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg
     2161 gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa
     2221 gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc
     2281 atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata
     2341 atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg
     2401 tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt
     2461 aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt
     2521 tcgattccag aatgtaccag cgttcccatg gaataccgca ctaatgtaac cttgtgtttg
     2581 acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga
     2641 gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc
     2701 tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact
     2761 attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat
     2821 caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctc tagcgagccc
     2881 gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat
     2941 atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata
     3001 aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt
     3061 cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc
     3121 taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt
     3181 ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg
     3241 gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat
     3301 cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag
     3361 caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat
     3421 ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa
     3481 gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt
     3541 caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg
     3601 tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcct
     3661 gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt
     3721 caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa
     3781 tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg
     3841 tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc
     3901 actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt
     3961 agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat
     4021 cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt
     4081 tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg
     4141 tatattcaat ggagaaggag acgggatcgg cggtctgatt gttgattatt atgcaggtta
     4201 tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc
     4261 ttttcgtgaa atctacccgg aaatcatagg aggctatgag aaaattcgat ttgaatcagt
     4321 aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa
     4381 agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt
     4441 agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt
     4501 gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga
     4561 aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt
     4621 gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa
     4681 atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc
     4741 cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag
     4801 tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc
     4861 gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga
     4921 acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg
     4981 caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag
     5041 aatggttcgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct
     5101 tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc
     5161 cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc
     5221 agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg
     5281 taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga
     5341 acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag
     5401 tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag
     5461 tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga
     5521 acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt
     5581 gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta ttattacgat
     5641 gtccatggga aatcttggta aaatttctcg tctggttggc ggaacgacgg gttccgttat
     5701 gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc
     5761 attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt
     5821 ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga
     5881 ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg
     5941 ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg
     6001 tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag
     6061 caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga
     6121 ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt
     6181 tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac
     6241 agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa
     6301 aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc
     6361 ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt
     6421 agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc
     6481 cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag
     6541 agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag
     6601 cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc
     6661 tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat
     6721 accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg
     6781 aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc
     6841 cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat
     6901 accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt
     6961 taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct cgtgaatata
     7021 cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac
     7081 tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc
     7141 agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat
     7201 tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag
     7261 ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa
     7321 catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat
     7381 atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg
     7441 tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat
     7501 cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc
     7561 agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata
     7621 cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa
     7681 aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc
     7741 gtggaacgat gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa
     7801 aggatcaagg gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt
     7861 cttttttgga cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac
     7921 cagaaagagt tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa
     7981 atagagggta ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct
     8041 tccatcctag tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga
     8101 atagtttaac cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag
     8161 gaaaatctga taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac
     8221 atatcggccc atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg
     8281 gctatttgtt cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta
     8341 aacctttgga tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt
     8401 attatgtcaa acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg
     8461 tttccggaag cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga
     8521 aagttgttac atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga
     8581 aactattgaa agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac
     8641 gtccttctgc tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata
     8701 tcgctttgcc atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag
     8761 aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt
     8821 ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag
     8881 tagcagtatc tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc
     8941 aagtggatga gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag
     9001 cagccgagta cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa
     9061 aagttgccaa agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat
     9121 attgtcagca ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct
     9181 gtagagtaaa cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag
     9241 tagcaccgtt tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc
     9301 acaacgaatt acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac
     9361 caacgaacta tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat
     9421 ctgattctga agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag
     9481 cgatcgactt cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac
     9541 cacaaatctt ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag
     9601 taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac
     9661 cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag
     9721 caaacaaacg tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag
     9781 atgcagaagg ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat
     9841 taactgctgt tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc
     9901 aaggtgccgc tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt
     9961 accaatacgc agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga
    10021 tcaactatga accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat
    10081 ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc
    10141 actctttagg acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg
    10201 ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag
    10261 gctacttgca aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc
    10321 ttgctcatac agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt
    10381 atacattggg ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga
    10441 atggaatcaa cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac
    10501 ttctaggcaa accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata
    10561 agggtttttc gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa
    10621 ctggtaccca tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga
    10681 caagacgttt gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag
    10741 tttgttttag gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt
    10801 tttctagaat cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt
    10861 agcttaagat aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac
    10921 ttttttctct atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta
    10981 agcagaacca ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc
    11041 accgttttca gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt
    11101 atctgctttg atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt
    11161 acctacataa gtagactgat agtagtccca acctttccat cctataaaag caatcaatat
    11221 gataacgatc aaccctaaaa tttttttcat gaaagatctc cttttttatt tttat
//
LOCUS       DACCCM010000086        11255 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCCM010000086 DACCCM010000000
VERSION     DACCCM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.090"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVK78_002343"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVK78_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP6375782.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVK78_002344"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVK78_002344"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP6375783.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVK78_002345"
     CDS             complement(2169..2906)
                     /locus_tag="IVK78_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6375784.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVK78_002346"
     CDS             complement(2997..3524)
                     /locus_tag="IVK78_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP6375785.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVK78_002347"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVK78_002347"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP6375786.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVK78_002348"
     CDS             complement(4401..4886)
                     /locus_tag="IVK78_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP6375787.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IVK78_002349"
     CDS             complement(5039..5236)
                     /locus_tag="IVK78_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375788.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVK78_002350"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVK78_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP6375789.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVK78_002351"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVK78_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP6375790.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IVK78_002352"
     CDS             9211..9423
                     /locus_tag="IVK78_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP6375791.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IVK78_002353"
     CDS             9425..11104
                     /locus_tag="IVK78_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6375792.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3266 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaata
//
LOCUS       DACCCM010000087        11080 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCCM010000087 DACCCM010000000
VERSION     DACCCM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.091"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(390..1337)
                     /locus_tag="IVK78_002354"
     CDS             complement(390..1337)
                     /locus_tag="IVK78_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP6375793.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="IVK78_002355"
     CDS             1525..2712
                     /locus_tag="IVK78_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAP6375794.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="IVK78_002356"
     CDS             2693..3997
                     /locus_tag="IVK78_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6375795.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="IVK78_002357"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="IVK78_002357"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAP6375796.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="IVK78_002358"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="IVK78_002358"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAP6375797.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="IVK78_002359"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="IVK78_002359"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAP6375798.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="IVK78_002360"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="IVK78_002360"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAP6375799.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="IVK78_002361"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="IVK78_002361"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP6375800.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="IVK78_002362"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="IVK78_002362"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAP6375801.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="IVK78_002363"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="IVK78_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10485..>11080)
                     /locus_tag="IVK78_002364"
     CDS             complement(10485..>11080)
                     /locus_tag="IVK78_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6375802.1"
                     /translation="GDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISDAHKG
                     LVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKN
                     RLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKI
                     IRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3854 a         1727 c         2174 g         3325 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tagtttcttt
     2821 gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat acgtatttat
    10141 caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga atagaccaga
    10201 agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt gggcattcaa
    10261 gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac acaaaaataa
    10321 ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt ttacttacat
    10381 aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa aaaagtcaag
    10441 tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt ggcaaattta
    10501 atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac tgctccaatt
    10561 aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct tctgcgcact
    10621 tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt
    10681 acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat
    10741 tttggttgat cgatataatc atgaatcaat cgatttttag cctcacgagc taagttaata
    10801 tctgtgaact taaaaattcc tttaacagct tctctgaaag attttgaatt ttttttagga
    10861 atggtggtaa agatatttct taggaagtga acttggcatc tttgccaact tgcgttggtg
    10921 aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttctgta
    10981 ccttgtaaac cgcgttcttt taggtattca aaaaatgtta tccaggtctc ttcgctttcg
    11041 ccactttgaa tcatgaagcc gataatttca cggtcgccat
//
LOCUS       DACCCM010000088        10510 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCCM010000088 DACCCM010000000
VERSION     DACCCM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.092"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK78_002365"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK78_002365"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP6375803.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IVK78_002366"
     CDS             1085..2005
                     /locus_tag="IVK78_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6375804.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IVK78_002367"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IVK78_002367"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP6375805.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IVK78_002368"
     CDS             3311..4789
                     /locus_tag="IVK78_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6375806.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IVK78_002369"
     CDS             5066..7126
                     /locus_tag="IVK78_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375807.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IVK78_002370"
     CDS             7209..7778
                     /locus_tag="IVK78_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6375808.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IVK78_002371"
     CDS             complement(7953..8759)
                     /locus_tag="IVK78_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375809.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IVK78_002372"
     CDS             8897..10411
                     /locus_tag="IVK78_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP6375810.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1752 c         2071 g         2961 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DACCCM010000089        10218 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCCM010000089 DACCCM010000000
VERSION     DACCCM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.093"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..410)
                     /locus_tag="IVK78_002373"
     CDS             complement(<1..410)
                     /locus_tag="IVK78_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6375811.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEK"
     gene            complement(492..842)
                     /locus_tag="IVK78_002374"
     CDS             complement(492..842)
                     /locus_tag="IVK78_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375812.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IVK78_002375"
     CDS             complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IVK78_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6375813.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1481..2146
                     /locus_tag="IVK78_002376"
     CDS             1481..2146
                     /locus_tag="IVK78_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6375814.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            2136..2591
                     /locus_tag="IVK78_002377"
     CDS             2136..2591
                     /locus_tag="IVK78_002377"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAP6375815.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2741..4120
                     /locus_tag="IVK78_002378"
     CDS             2741..4120
                     /locus_tag="IVK78_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAP6375816.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4257..5099
                     /locus_tag="IVK78_002379"
     CDS             4257..5099
                     /locus_tag="IVK78_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6375817.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            5173..6621
                     /locus_tag="IVK78_002380"
                     /pseudo
     CDS             5173..6621
                     /locus_tag="IVK78_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6736..8508
                     /locus_tag="IVK78_002381"
     CDS             6736..8508
                     /locus_tag="IVK78_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6375818.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            8483..9775
                     /locus_tag="IVK78_002382"
     CDS             8483..9775
                     /locus_tag="IVK78_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HAP6375819.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         3534 a         1665 c         1935 g         3084 t
ORIGIN      
        1 cttttttctt tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca
       61 aaggttttat ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct
      121 gctcgaccaa taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct
      181 tgttgcccat ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat
      241 tgttcatagt agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg
      301 gtttgtcgag aacgttgtac tagatgggct acttttgaaa caaagtgtga ttgattgaaa
      361 taagcttcta tcatcacgag ttcagttggt gtaagatggg tataggtcat ttgtactcac
      421 tccttataat tctttcgacg gaactatttt gagtgtagca caaatgactt ttttagttgt
      481 ctagcttaat tttagaattg gcgtatgctt aaaatgcggt tataggcttt tgtatgaacc
      541 atttcagtaa attgaaaatt atttatgatt gcaatctctt gttgggaacg tgcactatct
      601 cgaataattt ctcctaattc attggattga taggtagcga tattagtcaa aagaatcaac
      661 aagcgattga gctgctcttg ttcttgtaat tgtaaatttg cccattttga tcgatcattt
      721 tcgatgggga cacgtgtatc caaccaaaaa agtgaggttg ctctttccca tacaacgttg
      781 tctaattcat cctcaatgac attccagtta attgctttat agtgtaaata attatttatc
      841 atagtacctc ctaaaatatt gtttgaaagg ggatgatatg gtgtcatttt ttattgcatt
      901 gtttctgcaa ttctctctat atcttctgtc gtacctttta gttcaaattc atccaacacg
      961 gtaaaaccaa aatcatcggc gtattgatag gctgttaggc aaaattgttt attgaagttt
     1021 ctgttgccac taccaataat tcctaaacaa tagtctcggt ttttattgta agtaatcact
     1081 tggcgcaatg gtgttgtcaa aatttcgaca tctcctgttt caataccatt tccaccttca
     1141 agataagtag gcagaaatac aaaataggat tcattgatgt caaaagtgag tggttttcct
     1201 aatttgacta aatcttttac attcacataa tctaccttct tttgatattg tttttgacaa
     1261 tacgtatcta aatctgcgag aaaactcgtg gtatttccac ttaaactaat ataatagact
     1321 ttaattattt gcatgtatca cacctctttt taataaataa ccacatatat agtgcttgtt
     1381 ttaattagaa acgctatata tagtgtttat tgtacatgac atatcgttat agaaaattga
     1441 cgatggtcaa ttattttgat tttagaggag agaaaattac atgaaaaatt caggttgttt
     1501 atgcaccgtt ccactcttcg cctctctttc attagatgaa aaaaatgaga taaagtgttt
     1561 gcttcaccat cagacgttta aaaaaggaga aattattttt tcaccaagta gtgaagaaca
     1621 actgttgatt atatctttag gggctatgaa aatatatcga attagtagaa ctgggaagga
     1681 gcaattaatt cgtatcgtaa aagaagggga ttacgacggt gagaactatc tctttggtct
     1741 acccaatgac tgcctatatg gtgaagctgt tgaagaaacc aaaacttgca ctctctataa
     1801 atccgacttt aataaagtga tggcacgcta tccacagctc agttatcgat tgttacaatt
     1861 aaatgcttgc aaaacagtag ctgtggaaaa acagttgcag cttctagcgt ttgatcgtat
     1921 agaggaccgg ttagccttat atcttaacca actatctata gcagcaaaaa tgggggagcc
     1981 tattacttta cctatctcta tgagagagtt ggcaaactat ctagctacca gtcctgaaac
     2041 tatttctcgt aaattaagag attttcaaga aagacaattg attactagaa aaggaaagca
     2101 gattacacta ttagaagaat tttggtttat atttaattat atataaagaa gaagatatta
     2161 aaattgggat tgtcattgca cgattcaatg attttttaac taaaagctta ctaagtggtt
     2221 gtgaagatgg ccttacgcgt tcaggcgttt tgacagaaaa tatcgatgtt gcttgggtgc
     2281 cgggggccta tgaaatccca cttacagccg caacgatggc tgaaacaggt aaatatgatg
     2341 ccattattac cctaggtgcg gtaatcagag gagctacaag tcactttgat gtggtggtta
     2401 accaagcgtc aagtggggta tctcaagtag gcttaaccca taaattaccg gtcatctttg
     2461 gtatcttaac cgttgaaaac atcgaacaag ccattgaacg ctctggtact aaagctggta
     2521 accttgggtt tacttatgca acgaatacta ttgaaatgat taatttattc aaagaaatta
     2581 aacaacaata agtcatcatt ctatatgaaa atatctgtaa cagcataggg ctagctttaa
     2641 ttttcaatca tggtaagatg gaagatagga aaataaagcg cattcaatta attatcaagc
     2701 ctttctgatg atttgcgcaa aaagcttaag gagacttcat atgacgattc aatttaaaca
     2761 aacattagcg ccaaccaagg ataattctgg aaaaccaatc attttaagtg acgctacctt
     2821 cgctcaacgt aatgagaaag tccttcagtt aatgcgtgaa tatgacttct catcattgat
     2881 gatttatgca gataaagaac acggtagtaa ctttgaatat ttaactggtt ttattccacg
     2941 gtttgaagaa gctatccaag taatcaacca agacggaacg tcgacactga ttttaggtaa
     3001 tgagaattac aataaaacca gtatggcacg tacacaaagt gaaggtattc tttgcccatt
     3061 cttctcttta ccaaaccaac ctatggattt cactaaaact tttgctgact atcttaaaga
     3121 cgttaaagta gatgcttcaa aacgaattgg tttagtagac tggaagcttt tatctaatga
     3181 tatggacaac ttccacaacc atcattcaat accagcattt attgtagatg gtatacatga
     3241 agttttcggt tctgacaagg tagtgaatgc aacccaacta tacatgcatc caaaagaagg
     3301 tgctcgtgtg acaaacaacg cagaggaaat cgctcgttat gaatatggtg cttcccttgc
     3361 ttctgatgct gttcttgaag caatgaacca gctaaccgtt ggccaaagtg agttagcagc
     3421 aggtaatcac ttaaatcgcg atggtcaata tccatctgtt gtgaccattg cagcctttgg
     3481 tgatcgcttt gagggtgcta atatttatcc aactgataat acgctaaatg atggcgataa
     3541 agttgcatta actgtagcct ataaaggagg cttgtctagc cgctctggct atgcagtaaa
     3601 agaggaaagt cagctcgctg aacatgatcc aggatactta gaagaagtgg tttacccata
     3661 tttccaagcc tataaatggt ggctagataa tttaaccatt gggagaaagg gtggcgattt
     3721 ctaccaagca ttcagtgact tctatccgca agctacttat ggctggaatt tgtgcccagg
     3781 gcatttagta gctgacgaag aatggttaag ctcaccattt tatcaagact ctgatgcaac
     3841 aattcaaagt ggctacatct tccaactaga ctttattcct tcacaagcag gccacaatgg
     3901 tgtttcagct gaatcaacga ttgcggttgc ggacgcggca ctacgtgaac agattaaggc
     3961 tgaacaacct gaattatggg cacgtatctg taaccgaaag gattatctaa aagaagcatt
     4021 aggtttagaa ttatcagaac atttactacc cttagcgagt acctttgcct actatagacc
     4081 tttcttatta gacaaaaaaa ctgctatcta ctttggataa aaaaatgccc ctcaagattg
     4141 tttgccaaac ttgagggttt tttgtctatt tttaagtgaa taatgacgga ctatccaaac
     4201 ggaatcgcca taaagatgtt aatatatgta taatattata aagaaattgg aggacaatgc
     4261 ccttggacga gcagattgaa ttattattta ccatagatga aggctatttg aatcccttaa
     4321 aagtggcttt aacatcgatt cgtcagaata atccaggcca agctttccgc atttggttga
     4381 ttcacgaatc cattgcaatg catacgattc gcgaattgca gaagctaacg gattatctac
     4441 agtttggttt tgaagcgatt aaaattgatg gtagtcgatg gaatagcgct aaaactgaag
     4501 atagataccc aaaagagatg tatttccgcc tgcttgcggg tgaaatatta cctaaagaaa
     4561 tgaaacgcgt gatctattta gacccagata ttttagtggt taacccttta ttagatttgt
     4621 ggcaaacaga tttagaaggt tatatgctag cagctgctac acatgttgga ttgacggacg
     4681 tttctacacg agtaaaccaa atgcgacttg acgttgacca tgcttattac aattctggtg
     4741 tgatggtgat tgatttagat aaagctagag aaattattaa atggtcagat attgcacaaa
     4801 tgattgaaaa atataacttg ctattaatcc tacccgacca ggatatcctc aaccgattat
     4861 atggtaaata cactaaggaa attccagaag aaatttggaa ttatgatacg cgtaaatata
     4921 tgcggtattt caccaaaagt ctcgctcaac atgatattca ctgggtgatg gcgcatactg
     4981 ctattctcca tttctgtggt ggcccaaaac catgggacga taaacatgat aatcgtttta
     5041 ccagtcttta tcttacctat caaaatcaat taaagcgtat tgaagatttg atgaagtaaa
     5101 gtgaaacaag tgtttttcat tacctacggt ggtttaatgc ttatttatta tcgttaaatc
     5161 atgcttatac actttacaaa atttgatttt cgaaatggcc gtccaacgaa gtctgcgatc
     5221 ttaaccacga gttccattga catggccaaa aagtattatc gagcgatcaa agaaatgacg
     5281 aaagaacctg actggttaac aaaagagttt cctaatcagc ccattcgtga aggaagaacg
     5341 atggaggatc ctgattttcc acgaatcgct attacctatt cattagaaga aaataaccaa
     5401 gattcaagtg cccaacaaga agaaatgaaa aaaattattg aggaatataa tcagtatttt
     5461 gatactgcct ggtcattagc agacattgag cgttataacg gagacatcaa taattgtttg
     5521 gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa tcgatttagt aatcgtagtg
     5581 gatcgtctat tgacaggttt tgacgcacct acgattcaaa ccctatttgt ggatcgtaat
     5641 ctagagtatg caggattgat tcaagctttt tcacgtacga accgtacgta tcctgaaaag
     5701 acaaaaggat tgattgtgac gtttcgtaag ccagcgacaa tggagaagaa tgtcgaggat
     5761 gcgacaaaac tgtattcaga agcaaaagaa gaatcgggtt tgatttatcc aagttatcag
     5821 gaatccaaaa aaagattcaa gcaagcgtat ggtaagttaa aggaggtttc atccatgaaa
     5881 tccattgatg aacatacgcc acttgaaatc cgtatcgagt acgttaaagc atttcaagaa
     5941 ttgaatcgtg cgtatgaatc gttagtgact tatgacgatt acaatgatga catggtgcag
     6001 tcttcaactt tgaacaatca aattcaactt ttggaagaac aaattggcgt gtacgagacc
     6061 gtgaaggggt cattaataga ggaagaacca gaaaaagagg gagaagattt ttcaacgatc
     6121 gaattttata gtgaaaatac cactaagttg tatgatattg attctactta tattgatcaa
     6181 ttactaggaa cttatgctgc gaatagttca gatgcacgag atgaaattga aaaagctttg
     6241 gctaaattga ataaaacaga aggagtcaaa gaagtttacc gacaaatttt aaatgcattt
     6301 gatgtgggga ctttagatag caatgaagat atttttgtca ttaaacgtcg ctattttaca
     6361 caagcaagtg atgaattgat tcatgccttt tccaatgagt ggtttgtttc tgagaaagaa
     6421 ttacatgctt ctaatctgca atatatgcca ggtgaagatc caattcctaa tatgaaagca
     6481 attatcaaca gtaaggatta cgaaggatat aaagccaaac atccaaaagc gaagccattt
     6541 aaatacccac aagaaatgaa gcgagcctgg cgtaaaatgt tagatgatga gttgattcca
     6601 cttgagaatg aattgaggta aacgagaaaa ttgaagaaat tgtatgataa acatgttaat
     6661 aaatgatgct cgaaataaaa tttggtttta tttttacatt gatacggtaa ataaaatttg
     6721 aaagtagaag aaaatatgaa caacaatgaa acaaaaaaaa catatgaact aagaataact
     6781 gctgatgaga ttaaatcaac agacccaaca tttactatga agaatctaag cttaggggat
     6841 aattatcaac caaaagatgc ttacttccgt acggcagaag gcgaaataac tagaagaaac
     6901 tacggtaaaa atgaacggaa aatttcaaat caaacattac cgcgattagc tattcaaatg
     6961 tttgaacaat cgattgtttc tctaccagaa agcgaaagag caacatttcc tgtgtgtcga
     7021 tatcactcag gagatgagat aatacgaggg atttttccag atgttgacag tttcaaaaaa
     7081 aaaaggaagt ccattgaatt tttgatttat aaatataatg aagacaagca gatagtcatc
     7141 tattgttgga gtattttttc aactttatta tttgtacaag aatgtttgaa acgttttgga
     7201 acatctgggg atgaatttat tcttgtttat aaagggaaag agaaaaagga aatacgagta
     7261 ggagagactc agactatgac caatcatttg ctgaaatcat atgttttaca tttactcaaa
     7321 tctaaaaacc ttattctacg tggcgctcct ggaacaggga aaacattcct tgcaaatgaa
     7381 atggcagctg atgtcgtttc aaatggcaga actacacaaa tcaatgaatt gagcgaagat
     7441 gaaaagagcc gaattggatt tgtccagttt catcctagct atgattatac tgattttgtc
     7501 gaaggtttac gtccaactac atccgatgat ggcgttgtta gtttcacctt gaaagatgga
     7561 agctttaaga gctttgttga aaaagcaaag aaaacaaaga aagtaaatgg acaagataat
     7621 tttgaagaag cttgggctaa atttttcgat gcggtaacgg aagctagtat agagggagaa
     7681 ggctacaata aacttaaaac attgacaggt aaaccagtac gcaatcttct agcctatgaa
     7741 agaaatgaaa ttcaaggtgt ttatcctgct aaaaccaata cttatctaaa ccatgaccaa
     7801 atttataatg tctatcgtgg attgccaggt acgccaaaag gtggatttga tgcttatcga
     7861 aaagcgattg taaaacattt aaaagaagaa tttggcttaa aaaactacac ggcgtcaacg
     7921 gtagattcta cgaataacca aaactatgtg tttatcattg atgaaattaa tcggggcgaa
     7981 atttccaaaa tttttggcga attatttttc tcaattgatc caggatatcg tgggcataaa
     8041 gaaggtgttt acactcagta tgcaaacctc catgaaaatc cagaagaaaa attctatatt
     8101 cctaataatg tttatattat tgggacgatg aacgacattg accgttctgt tgacactttt
     8161 gattttgcta tgcgccggcg atttactttc ttagaaataa aagcagaaga ttcagcacaa
     8221 aatatgctga ctcaagagaa aacaaaagaa cttatgacgc gtttaaatga ggcgatcgtt
     8281 gccaaagaaa aaggtggatt gtcaactgat taccaaattg gtgctagtta ctttttaagt
     8341 atcgataatg gtgaggagac aatggaacaa ttatgggata ctaagttata tccattattg
     8401 aaagactatt ttcatggtga acataaggcg aaagaaaaat tagaaatact agaaaaggta
     8461 tactctaatg acggtgattt agatgaagct gaaacctcta cacgataatc aagaaatcaa
     8521 tgactcagtt gtgcaacatc caatcattac gaacttagtt aatcgtgact taagtagttt
     8581 agagaaagaa aattttatta tctttccgca acagttaaat caatccaagg atctaagtga
     8641 agataacttg atatttcaat caagaaatgg aaaaatttgg acttgtaatg tcgtaggtat
     8701 ccttagcgat tcaacagatg aattaagaat tcattctaga ttcgctaatc aaatgaagca
     8761 tgaagatttc tttttacgtt atatgcttca gcgtgttttg aattacaacg tgattaatga
     8821 tcattttagt gcatctaaaa agatgacata ctacgatttg ttggtatttc tctttcctta
     8881 ttatctgaat gaagcaatgc gtaaagggac atacaaagaa tatgtcaaac gagaatacaa
     8941 taatgccaat atcaagggag ctgttgatgt tgcaaaacac atccgttcga atgttccttt
     9001 cgttggaaaa gttgcttatc gaacacgaga gtttagctat aataatcacc taacccagct
     9061 tattcgtcat actattgaaa aaatccaaaa tgagtacgat ttcttactta gtggagatga
     9121 ggatacgaaa gaaaacgtgg ttctaatcaa acaaacaaca cctggttatg ctagattaaa
     9181 tcaatttaat atccttcaag agaatatcct tcatccagtc aaacatagtt attacgagga
     9241 gtattcggct cttcaacaaa tctgtatcca aattttatcg gaagaaaaat caggatttgg
     9301 aagtgataaa aatcaaatac atggaatcat tatcgatgtg tcttggctat gggaagaata
     9361 cattggaaaa gtcactggtt ggaaacatta tggcagagat aaagggttag caacaatgca
     9421 tttatttcaa gagcccaacc ggtccccacg atatccagat tttacattta ataatatacc
     9481 aattgataca aaatacaaaa aacatcttga tactagaaat gactataatc agttagtcac
     9541 atatattcat ataatgaatt tagaccaagc agatacaatg aaaggcggct ttctgcaacc
     9601 tacttccgat ccccttcctg caaatcatgg gtataaaaaa ataggcatat taagcgggct
     9661 aggaggagaa atattcacat atcatttttt cattccgcaa gctgcgtcaa gttatttgga
     9721 atttgtagaa cagatgagag aggtagaagc agcactacaa actacttttc aataagtagg
     9781 aattatagaa attttactca aaaaatcttc attaaaaaat ctgactaaat aagttgagaa
     9841 aacagaatat acttcaatat ccagtcattt ataaatatca ttcactcatg atacccagct
     9901 ccgtatcaca aataatgata ttttttaaga gaaaaaatta tccctatttt aaataatagc
     9961 aataggtagt ggtagagaga aatttataaa taactttgtt atttgctttg gtagccaagt
    10021 aagttgcaaa agaaaaatag atactgttaa aaagataatg tagacataga aacaaatacg
    10081 taaaatttta gttagaatga tggcaaggag aaaaaacatg atagatgaaa aaaagtgttt
    10141 aaaaaatcta ttggaagata tggatgatat tgaagaaaaa ctatctaaat ggactgggaa
    10201 aacaaatata tttcagaa
//
LOCUS       DACCCM010000090        10201 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCCM010000090 DACCCM010000000
VERSION     DACCCM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.094"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            154..384
                     /locus_tag="IVK78_002383"
     CDS             154..384
                     /locus_tag="IVK78_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP6375820.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            481..1782
                     /gene="murA"
                     /locus_tag="IVK78_002384"
     CDS             481..1782
                     /gene="murA"
                     /locus_tag="IVK78_002384"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6375821.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1795..1971
                     /locus_tag="IVK78_002385"
     CDS             1795..1971
                     /locus_tag="IVK78_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6375822.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2106..2402
                     /gene="yidD"
                     /locus_tag="IVK78_002386"
     CDS             2106..2402
                     /gene="yidD"
                     /locus_tag="IVK78_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP6375823.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2587..3054
                     /locus_tag="IVK78_002387"
     CDS             2587..3054
                     /locus_tag="IVK78_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6375824.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3139..4023
                     /locus_tag="IVK78_002388"
     CDS             3139..4023
                     /locus_tag="IVK78_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6375825.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4140..4373
                     /locus_tag="IVK78_002389"
     CDS             4140..4373
                     /locus_tag="IVK78_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP6375826.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4461..4541
                     /locus_tag="IVK78_002390"
     tRNA            4461..4541
                     /locus_tag="IVK78_002390"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4495..4497,aa:Tyr,seq:gta)
     gene            4548..4619
                     /locus_tag="IVK78_002391"
     tRNA            4548..4619
                     /locus_tag="IVK78_002391"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4580..4582,aa:Gln,seq:ttg)
     gene            5134..5841
                     /locus_tag="IVK78_002392"
     CDS             5134..5841
                     /locus_tag="IVK78_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6375827.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5900..6544)
                     /locus_tag="IVK78_002393"
     CDS             complement(5900..6544)
                     /locus_tag="IVK78_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP6375828.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6670..6957
                     /locus_tag="IVK78_002394"
     CDS             6670..6957
                     /locus_tag="IVK78_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375829.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7065..7385
                     /locus_tag="IVK78_002395"
     CDS             7065..7385
                     /locus_tag="IVK78_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6375830.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7466..8779
                     /locus_tag="IVK78_002396"
     CDS             7466..8779
                     /locus_tag="IVK78_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6375831.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8776..9495
                     /locus_tag="IVK78_002397"
     CDS             8776..9495
                     /locus_tag="IVK78_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP6375832.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9623..10174
                     /gene="raiA"
                     /locus_tag="IVK78_002398"
     CDS             9623..10174
                     /gene="raiA"
                     /locus_tag="IVK78_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP6375833.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3403 a         1683 c         2179 g         2936 t
ORIGIN      
        1 cagtcccttt ttttgcatat ttttagcatt tatttcctaa aaaatgtaag gggattccta
       61 tatttttaag tatacttgtt tttcctttta tacattttat ggtattatca ttttattagt
      121 tattataaaa caatcgttag aaaggacgtc attatgcaag tatacggaat tgatgcggtg
      181 cttcgaatcg tgagtcattt ggcgtttatc tacttagcct tctggtcttt acgttcgtta
      241 cgcattgaaa cttttttcag accaatgcat aatacgcaaa ttagaatggc aatcgtactt
      301 ttttcaattt ttttagggta cactgcaagt aattttttct tagaagttat cgctttatgc
      361 cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta ctattttaaa agcaaggatg
      421 ttataataga aaagattttg agtaagtgta tgctcattgg aattttttgg agggaacaac
      481 atggaagaga tcatcgtaag aggtggcaat caattaaatg gaacagtaag aattgaagga
      541 gctaaaaatg ctgttttgcc tattttagct gctagtttgt tagcagaaga aggtattacg
      601 acattagaca acgttccgat tctttctgat gtcttcacta tgaatcaagt gattcgtcac
      661 ttaaacgtag atgtagattt tgatgaacaa aagaatcaag taacaatcga cgcttcaaga
      721 caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa tgcgtgcttc aatcgttgtg
      781 atgggtccgc tacttgccag aaatggacat gcaaaagtag ctatgcctgg tggatgtgcg
      841 attggtaaac gaccaatcga tttgcattta aaaggatttc aagctttagg agcaaaaatt
      901 attcaaaaaa acggctatat cgaagcaatt gcggatgaat tgatcggtaa cacgatttat
      961 ttagatttcc ctagtgtagg tgctactcaa aatatcatga tggctgccgt aaaagcaaaa
     1021 ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa tcgttgattt agcaaacatt
     1081 ttgaataaaa tgggcgctca agtctatgga gctggtactg aaacaatgcg tattgaaggc
     1141 gtggatcatt tacatgcagt caaccactca atcgtacaag atcgtatcga agcaggtacc
     1201 tttatggttg ctgctgcaat gacacaaggc aatgtgctga ttgcagatgc tatctctgaa
     1261 cataaccgcc cattgatttc taaattgatc gaaatgggtg cagaaatcat tgaagaagaa
     1321 ggcggagtcc gtgtcatcgg accaaaacat atcttaccta cagatgtaaa aacgatgcct
     1381 catcctggtt tccctacaga tatgcaagct caaatgacag ctatccaatt agttgcagaa
     1441 ggtacaagtg tagtgactga aacagtgttt gaaaatcgtt tccaacattt agaagagatg
     1501 cgccgtatga acgctcatgt caaaatcgac ggaaatgtag caatcatgga tggaaaccat
     1561 gaattgcaag gtgcagaagt atatgcaaca gacttgcggg cagcagcagc attagtttta
     1621 gctggcttga aagcaaatgg aatcacacgt gtgcgtaact tgaattatct agaccgtgga
     1681 tattataatt tccatatcaa actacagcaa ttaggtgcag acgttgaacg tgtagacatg
     1741 gatcaaactt ctgctgaaaa aacagcacaa acgattgctt aaggaagtgg ctgaatgagt
     1801 aaccaacgat atatcttatt aactttgata aagattttaa tggtgatctt attgcttatc
     1861 cttctttttg tggccggaac gatgatcgga tatggtgtca tcggtggcgg caatccattt
     1921 aaagtatttc agccaagttt gtggattcat attcgagact tttttcattg ataaataaga
     1981 atcacagtgg ttctagtcga tatgaagtga gccatgaagc agaacatgat aaaagaaaac
     2041 agaagggaac gttgacgtta gtcgcgatcc ctttttttag taaaagaaaa gaaggaatct
     2101 aattgatgaa gaagatattg atttatttag ttagaggata tcagcgcttt atttcgcctg
     2161 catttccacc tagctgccgt tattacccaa cctgttccaa ttatatgata aaagcaattc
     2221 aaacccatgg cgcgctaaaa gggacggtaa tggggactgc acggatctta cgctgtcatc
     2281 cttttgtcaa aggcggtatc gattatgtac cattgacatt ccgattaacg cgaaataaga
     2341 aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa tacgggtagc actgagcgat
     2401 aaagaaacaa tttggaaaat agcttcttat attttgcaaa attgtgagtt attgccgaag
     2461 tgttggattt tgaacaccgt caaaaaaaag gttgtgaaaa atacggctag cactgagcga
     2521 taaagaaaaa gaggaaattt attgtcaaag taacaactcc gcaactactt atatggggga
     2581 caaaaaatga tcgaagaact aaacaagaaa gatttaccat ggtccttgtt attagatgca
     2641 gatcctgata aagaaaaagt tcaagtatat gtttccagag ggagtggctg gatatggaaa
     2701 gaaaaagaaa aaacaatagg tgtactgatc tatgtggcaa gggaaaaaga atacgaaatc
     2761 gtcaatgtag cagtaacccc aacgtatcaa ggaaagggga ttggcggaaa attacttgag
     2821 acagcttttc agaagctttc acaactcgct tccagtcaaa ctcagatcat catacgaacc
     2881 ggcagtacta gcagtgcggc gttacatctc tatcaaaaaa tgggctatgt ggagattggt
     2941 cgagtcaaag actattttat ccatcattac tctgagccca tttttgaaaa tggagatcaa
     3001 ttaagggatc aagtgatttt agcaattcac ttacctaaaa atagctttaa ctaataaaat
     3061 ttcaattcaa gaaaatgctt tcaaatgcta aatagataca ttacaatagc tttagcaaac
     3121 aaaaaggaga atatttttat gggaatatta gtttttgatt ttggtggatc ggctgtcaaa
     3181 tatggttgtt gggatggcaa agagattaaa ggtaagggac aatttgcaac gcctgaatct
     3241 tgggaagaaa tgaaagcgca attatttcaa gtttataaaa aaatactcgt ttcttttgaa
     3301 gtcgaaggaa ttggaatcag ctctcctgga gtagtgaata atgagaaaca ggtgattgag
     3361 ggaatcagtg cgattcctta tattcatgga tttaatattt tccgagattt ggaaactctg
     3421 tttcaacttc cagtaacgat tgaaaatgac gccaactgtg ccggaatggc tgaattttat
     3481 gaaggtgctg cccgcgatta tcaagatgtt gcttttgttg tggcaggaac tgggattggc
     3541 ggggcgttgt tcacgaaagg acaaatcaat aaaggggcac atctttacgg tggagaattt
     3601 gggttgatgt tcttaaatgg cgataagaca ttcagtaaac ttggtacagc ggtacagatg
     3661 gcttggcgtt attgtgaacg caaaggtctc gataaaaatg cttataccgg aaaagatgtc
     3721 tttgaattag cagaaactgg agatgcaata gcaaaagaag aagttgagag tttttatacg
     3781 tatcttacaa agggattatt cagtattcaa ttttcgtttg atccagaagt gatcgtatta
     3841 ggcggcgggg tttctgcaaa agaagggctc attgatgaga tcaaaagccg aatgaagaag
     3901 ctgacagaac aattcgatct gcatgatttc gaaccgcaga ttttattatg tgaatacaga
     3961 aacgatgcta atcttgtagg tgcagcagca aactatctcg ctgtacgtca aggggagaat
     4021 taagagcttt agagaaggtg cagcttgcat aaaagaagtg aatgaaatga ataggagatt
     4081 tttacctaag gaatgtttta tttgctgcag tatgataaaa tatctgagag gtgaaaatga
     4141 tgtcaaaaaa aaataaagat attgaagtgc gtatagaaga agtgaaaaaa gatatcaaag
     4201 gtaagaatta tgaagtcact cagttattta tagggaaaaa aatgattggt gagatcttag
     4261 cctatggacc aaaagaatat gaaattttct ttggtgaaga agattttgga aaagaaaaaa
     4321 gtcttgaaaa tgcgatagaa acagtgattc gacactggaa tctgcacgaa taaaaaaata
     4381 tcaaaaaaac tttcaataaa gggttgcaaa aatctggaaa attaggtatt ataactaagt
     4441 caggttgata caaacctttc ggaggagtag cgaagtggct aaacgcgacg gactgtaaat
     4501 ccgttcctta gggttcagtg gttcgaatcc actctcctcc atttcattgg ggtatagcca
     4561 agcggtaagg caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt
     4621 ataccacaat cgtaagctct cgttagttcg agagctcttt tttttgcttt gctaccttgt
     4681 tccttgaaga ttctgctgat ttaagataga gttaagttaa ctacaagaat tggagggatc
     4741 cacatgaaat ggaaagaaaa agtagacgaa gcagctgaaa agttatatga tttggtcaaa
     4801 tcagaaaaat acaatattga agtgaatatt ccgaaaaaag gcggaaaagc ggtccgggta
     4861 aaatcaaaac gcccaacgaa tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa
     4921 caaaagatcc ataagtcggc attttcatcg attttagggt cttttgttat tttctaggtt
     4981 ggacatatct aaaaaattgt ttctgacaat aggagtagga agaacgatgt gtgtctaaat
     5041 cctctgttga tggatagaaa cctttatctt tttcagttgt gaatcaggta tataccagtt
     5101 ataatagaag aaacgaaagg acgggtgtac gttatgtcga atcaattacc tgtatatata
     5161 cagattcatg accaattaaa aaatgaaatc gaacaaggta tatggaaagt cggagaccgt
     5221 cttccttcag aaagagaatt agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa
     5281 gcaattcaaa acttggcaga tgaaggaatc ttagaaagaa agatcggttc gggtacatat
     5341 gttgcacgag aaaaagtaca agaaaaaatg tctggtgcaa caagttttac agaaattatg
     5401 gaatcacaaa atcgagtacc gtctagtcgg acaatctctt atttcttgac ttcgccaagc
     5461 tcaagtgaaa tggaaaaact aaacttgagt aaagatgaca cgatcttgcg aatggaacgt
     5521 attcgtttcg cagatgatgt ccctatttgt tttgaagttg ccagtgtgcc acaaaagctg
     5581 atcgatggct acagcaaagc agagattact gaatcgttgt atcgcacgct agaagaaaaa
     5641 ggcgggaaaa agatcggtcc agcgaatcaa acgattactg ctgtgttggc ttctgagaaa
     5701 attgctgact atttagaggt aaaacgagga gatgcagttc ttcgtttgcg ccagatttct
     5761 tattttgaag acggtacgcc gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt
     5821 gagttttatt tggaaaaata aaaaacaggt gatccctgat tccgtattag aaataaaaga
     5881 gaatcagaat cacctgtttt tatatatgat cagttaccag ttgctcgtga taagaaacag
     5941 gaccctcttc aaattggact tgtccgttca gcagctcaat gatggattct ttaaagtggt
     6001 cagcactttt ttcatctaca gcacagatga cgctaacttg atcagtatat tggatttctt
     6061 ttataaaaaa atgttggagt tcaagataat tctgtaattt tcctagttgc ggatacgtga
     6121 tcatcacaga gatctcctgt tgcaaggtac cttctacgat tccaatgtca gacaatgctt
     6181 gagagaccgc atgagtgtat gctcgtatta atccgcctgt gccgagtttc gttcctccaa
     6241 aatatcgagt gactactgca gctacattga tcagttgatt ttttttgagg acttccaaca
     6301 tcggtatgcc cgccgttcca cttggttctc catcatcgct gcttcgctgg atttcatttt
     6361 tttctccaat gatgaaggcg ctgcagttat ggttcgcttt ccaatgttct ttttttattt
     6421 cttgaatgaa actttttgct tcgtcctctg attctattcg tctaagagaa cagataaagc
     6481 gtgatttttt gatctcgatc tcactttgtc cattttcttt tattgtacga tagcgctcga
     6541 tcatcttgtc acctcttttt tcattttctt tattcagtat aaataaagtt ctaggttgtt
     6601 tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca aaggcaggtg
     6661 gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac
     6721 aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct gtttcccagt
     6781 aacggacttc ccaaaacagc aagggaaagt acagattggt gggcaaatga ttataaacac
     6841 cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt catcaatttg
     6901 gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt tgattaaata
     6961 aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag taacagacgc
     7021 gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa
     7081 tcatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa atgcaaaaag
     7141 ctgctgaagc aaaaggaatc gactctgata ttttcgctgt gtccgcttct gacgcagata
     7201 acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt cgctttatga
     7261 aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc atcaatatgg
     7321 ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg ctcataggtg
     7381 aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt
     7441 cgtatatttt tttagggcga ataatatgaa tcatttttgg ggtagaaggc ttctgacgag
     7501 agatattaaa gcaatagact gtgaggaggt aaatttacca aattatgaga agatcgcagc
     7561 agtggaacgt ttgagcaact ggattctttg tcatcgctgt ggaatgagaa cgtccgtcat
     7621 tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt tagggcgatg
     7681 cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt
     7741 ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga gaatactttg
     7801 ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga aaacagagat
     7861 gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga tttgtacacc
     7921 gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc cagaagaaaa
     7981 aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt ttgttatctg
     8041 tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga tcgatgagat
     8101 cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa cagcgattaa
     8161 accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac taaaggagat
     8221 caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt tgcttcctga
     8281 acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga agaagataaa
     8341 atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt tattttgtcc
     8401 aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc cgagatatga
     8461 gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga aaatgcgaga
     8521 agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca catttgaaag
     8581 aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt ctttggtaca
     8641 aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat attttttcta
     8701 tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga
     8761 agggaagaag atactgtgag gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca
     8821 ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc agtttgtcgc
     8881 gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc aatccatttc
     8941 aatctagaga gtacgaaaaa gaaagtaaat tctatccccg tttactgcag tgactgtcaa
     9001 gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt tgtgtacgat
     9061 gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg actagcaggc
     9121 acctttgcat ataaatggac gcagataaaa aaagaatacc cgacatacat ctattgtcca
     9181 atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag cgagatgttg
     9241 aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct gctggctcaa
     9301 gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact tgcagtcacc
     9361 ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac aacaggacag
     9421 actcttttcc acgcagcaga ttgtttgcag atcgcacagc cgaaaatcat ccgaactatt
     9481 tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc aaataaccta
     9541 gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt cttgattgtg
     9601 gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa atatcgaagt
     9661 aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac gctatttcag
     9721 tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa
     9781 agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa cttctccaga
     9841 tttatacgca agtatcgatt tggttgttga caaattggaa cgccaaatcc gcaaattcaa
     9901 aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcaaatg ctgctgcttt
     9961 attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac gtacgaaacg
    10021 cctttcattg aaaccaatgg atagtgaaga agcagtcttg caaatgaaca tgctgggaca
    10081 taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt accgccgtaa
    10141 agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa
    10201 a
//
LOCUS       DACCCM010000091         9866 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCCM010000091 DACCCM010000000
VERSION     DACCCM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.095"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(74..679)
                     /locus_tag="IVK78_002399"
     CDS             complement(74..679)
                     /locus_tag="IVK78_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6375834.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(732..1706)
                     /locus_tag="IVK78_002400"
     CDS             complement(732..1706)
                     /locus_tag="IVK78_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP6375835.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(2084..2863)
                     /locus_tag="IVK78_002401"
     CDS             complement(2084..2863)
                     /locus_tag="IVK78_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6375836.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(2930..4201)
                     /gene="celB"
                     /locus_tag="IVK78_002402"
     CDS             complement(2930..4201)
                     /gene="celB"
                     /locus_tag="IVK78_002402"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6375837.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            4354..5751
                     /locus_tag="IVK78_002403"
     CDS             4354..5751
                     /locus_tag="IVK78_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6375838.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            5832..6272
                     /locus_tag="IVK78_002404"
     CDS             5832..6272
                     /locus_tag="IVK78_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6375839.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            6420..6746
                     /locus_tag="IVK78_002405"
     CDS             6420..6746
                     /locus_tag="IVK78_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6375840.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            6747..7496
                     /locus_tag="IVK78_002406"
     CDS             6747..7496
                     /locus_tag="IVK78_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375841.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(7576..8031)
                     /locus_tag="IVK78_002407"
     CDS             complement(7576..8031)
                     /locus_tag="IVK78_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6375842.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(8458..8733)
                     /locus_tag="IVK78_002408"
     CDS             complement(8458..8733)
                     /locus_tag="IVK78_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375843.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            9027..9842
                     /gene="thiD"
                     /locus_tag="IVK78_002409"
     CDS             9027..9842
                     /gene="thiD"
                     /locus_tag="IVK78_002409"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP6375844.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         3175 a         1730 c         1959 g         3002 t
ORIGIN      
        1 acgactttca caaaatctga tccagatcga tatctgcttc aggttgtatt atgtaagtcg
       61 tagttctttt cttttattta gttaagaggt ggtctgtcca ttccctcgct tgcttgattt
      121 cgtccatcgt caactgatga cctgttacag ttagctgaat agtcacattt gcttggcgtg
      181 tttcgaattg atcggccaat tctttaaagg aagcagggga aacgattgga tcgttatcac
      241 cataagaaat ccagacggtt ttatcctcta atgtggaacg gctttgatga acacctagag
      301 acatcggatg gaaaaacaac cctttattta gctcggtctc acgctccaat aataaatgag
      361 cagcgatgtt tgcgccattt gaatagccga caagcagcca gtcacttaac ggtatcagtt
      421 ctttttcgct gatttctttg attttttcat ggagtttatc tgtttctttt tccaaactgt
      481 ccaggtcgaa ttgattcaat ccattacgct tgaaaaaacg attcatccca tcttcttgga
      541 ccgtgccacg aaaagaaagc aatgtactgt ttggtgcaag aaagtgagcg atatccagta
      601 aagaatgttc atcgccacct gtgccatgaa gtaagatcaa atgcttgcca cttgttcctt
      661 tttcaaaaat gtaggtcatt ttgttccctc ttttctgttt ctatagatgc aaatgaagta
      721 gcatgaagag attattcacg ttttttattt gcatcacttg tatcgaaagg acggacataa
      781 gcttcaatgg cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct
      841 gattctgctg gttcatcttc aaggaatcca ggaccatcag ttgctaattc aaacagtacg
      901 tgatgagcgg ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt
      961 ggcaattcaa tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca
     1021 cgcaatgcta agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga
     1081 tgttcaataa tgatgcttgc accatttccc ccttcaccga tttcaaataa gtagaaatct
     1141 cctgaatggt ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct
     1201 aattgactga cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca
     1261 ttgctgtttt tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat
     1321 tgataatact gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg
     1381 attgctgaat gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg
     1441 tcacttggca cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc
     1501 tgatttggaa aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag
     1561 taaagatggt aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct
     1621 aaaatatctg taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg
     1681 tgaattcctc tgatttgtgt atccatgata aatttcctcc tgttaactta caaaaagtaa
     1741 gtagttgatt tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct
     1801 aaagtatcag gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag
     1861 acttacgact agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc
     1921 attacgggtg gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct
     1981 taggatagaa tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttaccac
     2041 tgtcacgatc tcttggtaaa cggctttttc acaacctctt tggttaagtt gaacgcacat
     2101 ccagcacaga atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct
     2161 gtccatcttt gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt
     2221 tcaaatattg ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg
     2281 caaaatggac attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt
     2341 tttttaaacc cggcacaaga tggactggca taaaggtatg ctctaaaaca tatggctcgc
     2401 catctaagat ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc
     2461 gttcttggat atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag
     2521 aacgatgtct gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt
     2581 ctttttgcat aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa
     2641 gtccttccat tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt
     2701 cttttaccaa gtcgacttga ttaggcaaaa aactatcgag aggatactct tttgaacgga
     2761 tacgttctct taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta
     2821 gagtataacg aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt
     2881 cgaaaaaaga ggtcatgaca agtttttgtc acaacccctt aatgatctat taagcaatat
     2941 ctaaatctgg ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga
     3001 caaagtcgat gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg
     3061 caatgattgg cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag
     3121 ccattaccaa ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga
     3181 ttgttgcatt caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac
     3241 caggtgctaa agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca
     3301 tcaataaaac gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca
     3361 tgaatggttt tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat
     3421 caagcatcag tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa
     3481 gtccgaagaa ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac
     3541 ttcccaaacc agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat
     3601 tgaaaatacc agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag
     3661 ggatcaaagc ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct
     3721 gccagccgcg tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc
     3781 caatgaacat tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac
     3841 cgccagcggt tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac
     3901 caccgaaaat accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa
     3961 caaaaaccga catgatgctc attgttgcat tttgcccatt accgaataaa ttcgataaag
     4021 ttccctttgt tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga
     4081 acatcgttaa agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt
     4141 gccctaataa attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca
     4201 tttttattca cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt
     4261 ttaaatgtct agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt
     4321 acggtatcaa gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat
     4381 ttttggtggg gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt
     4441 aaaggagaaa atatttggga tcattggtat gaattagagc ctgaaaaatt cttcgatcaa
     4501 gtaggacctg aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg
     4561 aaagaaactg gccataacac atttcgtaca tctatccaat ggagtcattt gattccagaa
     4621 gggacaggtg aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg
     4681 ctggataatg gtattgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg
     4741 caagaaaaag gcggatggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca
     4801 gcgtgctttg aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc
     4861 gtacctgtag aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa
     4921 gaagcggtcc aagtaggcta tcatgagaat ctagcaagcg caaaggtaat caaagcctac
     4981 catgaaggcg gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg
     5041 agagatgaaa cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac
     5101 cgatcattcc ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc
     5161 aaagaattgg ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac
     5221 acgattgacc tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact
     5281 ccaatcaatc acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc
     5341 aataaaaaaa tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt
     5401 ttaacgaata caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg
     5461 ggggtcgaag gagaagaacg atttgttaac agcgaaggaa ttattgaaga tgattaccgg
     5521 atcgagtttg ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat
     5581 tgtgtgggtt accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag
     5641 aatcgctatg gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa
     5701 tcaggtcgct ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg
     5761 ataaaacgtt cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg
     5821 aggcgtttac aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa
     5881 aggcagaaaa agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc
     5941 acatcctgta tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa
     6001 caagccttca ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact
     6061 tggcaattga aaaaggcctg acagatatcg agacatatgt tctttacggt tatccaaaga
     6121 cactgattgc taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga
     6181 caggtctgaa tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc
     6241 acgcatcatg taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa
     6301 gcataacgct tcttttgctt tctgttcctt ttattttttt gtaaaaaatt actttacctg
     6361 atgaagtaat catgctattc taattacaac gacagatgga gtaatatgga ggaaagaaaa
     6421 tgatttcaag tgatggaata cgcggataca atgatgcgat tattttatcg atccttaaca
     6481 aaaacgattc ttatggatat gaaatttcta aaacgattcg tgagaggaca aatgattcct
     6541 atgtgatcaa agagacaaca ctttactccg tttttgcccg tttagaaaag aatcaactga
     6601 ttgtttcgta tcctggagaa gtgactcatg gaaaaaagcg gacatattat aaaatcactg
     6661 aaatagggcg taatctttta gaagaaaaaa agcaagagtg gctgacgacg aaagacgtag
     6721 tcgatcaatt tttggaggga aattaaatgg atacaatccg taattattta gacagtcttt
     6781 ttatcggagt acctcaaagt accgagatag ataagctgaa aactgatttg ttggcaaata
     6841 tggaagacca ttatcatgaa ttgatgggag aaggaaaaaa tgaacaggag gccatcggta
     6901 cagtaatttc gacatttggt tcaatcgatg aattattaga agaactagat gtcgaaaaaa
     6961 aacatcaagc tgacgagacg gaaacaaaca cagcttctat ttatttaagc gaagcagaaa
     7021 actactggaa agaatacaga gcggcaagtc tccaagtggc atcaggtgtt ctattcatta
     7081 gcttatcttt tgcaagtttt cttttctttt gttcagcagg atatgtgttc atgggtattt
     7141 cttgtctgat tttcggtata gcattagctg tcggtttttt tattgcttct ggtatgaaaa
     7201 tcacacgttt aaatcacttt ttgcaccatc ggaaaatacc tgaaaaagta ttagcagaag
     7261 caaaagaaaa agaagaagag tatcagcgaa gttttggctt ctcactgatt gcaggaattg
     7321 gattatgtat tttttcactt ttcccacttt tggcttcttt aatgtggtat atggatggta
     7381 gtatcggtgc ctctatattt tttgttacgg taggaacggg tgtcttcttg atcatttatg
     7441 gcagtctagt ccgtcattct tatcgtcaat tcactcaaag cgcttattac tggtgaatgt
     7501 attcagattt gtaaaaatat tctatttatc cggcgtagaa aaaaagactg tgacacgctt
     7561 ttgtcacagt cttttttata tataataatt taaaaattca tcatctaaat ccgtagaatt
     7621 ttctagattg cttacccatg taaaatcgtt atagacagat ttcagttgac ttctgcgcca
     7681 ttgtacaggg ataaaaagac gcatgctatc ttcattgaat ccgactaata atttctttcc
     7741 gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat cgacaagctc
     7801 tgaggtcgta cattggtcga tttggtcttt caaattttta taatcaatcg agcgtttagc
     7861 taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct ctttccttgt
     7921 taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt ctttcagcca
     7981 gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca taaaaaaact
     8041 ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta aggagctgtg
     8101 cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa agtcttaaac
     8161 ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg cttcctgttt
     8221 gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga agaaggttga
     8281 tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact agatgaatca
     8341 cctaacaact atgattataa cttatagaac ttctgttttg cattcggttt tgttatagtt
     8401 gttataaagt tttattttat tacagaacat ttttaattaa taaagaacag ttagaagtta
     8461 aaattgctga ttcacataga gtttatgact tcttggaaat tctttttcaa tcagtttttt
     8521 catctgttta tgaaattctt ttttttcagt aaacataaag gtttgggtat cgtaaccttc
     8581 ttcatgtagg aaatagatta ttcctttttc tgtgttttgc gtataagctt ttatactcct
     8641 gtcttttttg aaagaacaga tcgtatagcc atctatttgg tctggtagtc gtttgatttt
     8701 cttcaaaatg acttttttat cctcaagttt catttttttc acctgacttt atttttttca
     8761 tatggaaatc atagcgcctt tggttatatg aaaacaagaa ggatactgaa atatatataa
     8821 atcaaatagt ggttatttat tattagaata gcgaatacaa ataaacgacc actattttct
     8881 gatttattgc aaatgttttt tcaaataaat ttgattacca agaaaaggta gtggtatatt
     8941 tactgaggga tactttgaaa aacatatgat agaaacagta acagtattat gaaaaatctt
     9001 tttataaaaa tggaggaatc gatttgatgg aagatcagta tgtacaagca atgacgatcg
     9061 caggatcaga tagcgatgga agcgcaggga tgcaagcaga tatgcataca ttctttacaa
     9121 gaaatgttta cggtgtttct gtcatgaccg catgtgttgc aggtaattct tacggaatcg
     9181 gagcaagtgt gacattgcca acagatttta tcgacaaaga atttgaactg attgcgaagg
     9241 actttcaaat acgtgcagct aaaacaggta tgctagcaga ttcaaagttg attgagacag
     9301 ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt cgtggatccc gtaatcgtaa
     9361 cgaaacatgg aaatctttta ctagaagaat ctgctttgca aagcttgaaa gaaaagcttg
     9421 tccctttggc cgaagtactg acacctaatt tttatgaagc agaaaagctg actggacgga
     9481 cattagcagc tgaagaagat tttgtgcaag cagcaaaaga actgcaagat atgggtgcaa
     9541 aaaacgttat gatcaaagga catcatgcta aaaatgcgca gcaagaaaca gtcaatgact
     9601 acgtattgtt cgaatcagga aaatcatttt ggttaaatgc accattttat cctaccgacc
     9661 ggatcaacgg aactggcgat tcgctatctg catgtattgc agctgagtta gcgaaaggaa
     9721 aaacagttga acaagcgatc cgattggcaa aagactatgt aaaccgtgca attcgtgaag
     9781 aaataaatgt agggcacaaa tatggcccca tcaatcattg ggcagctggt gaattagagt
     9841 aaaagaaaga agttgtgaaa aagttg
//
LOCUS       DACCCM010000092         9555 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCCM010000092 DACCCM010000000
VERSION     DACCCM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.096"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..344
                     /locus_tag="IVK78_002410"
     CDS             <1..344
                     /locus_tag="IVK78_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375845.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="IVK78_002411"
     CDS             425..1045
                     /locus_tag="IVK78_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375846.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1746..2030
                     /locus_tag="IVK78_002412"
     CDS             1746..2030
                     /locus_tag="IVK78_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375847.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2033..2950
                     /locus_tag="IVK78_002413"
     CDS             2033..2950
                     /locus_tag="IVK78_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6375848.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2943..3725
                     /locus_tag="IVK78_002414"
     CDS             2943..3725
                     /locus_tag="IVK78_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6375849.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3726..4406
                     /locus_tag="IVK78_002415"
     CDS             3726..4406
                     /locus_tag="IVK78_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6375850.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4399..5295
                     /locus_tag="IVK78_002416"
     CDS             4399..5295
                     /locus_tag="IVK78_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6375851.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5490..7139
                     /locus_tag="IVK78_002417"
     CDS             5490..7139
                     /locus_tag="IVK78_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6375852.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7733..8668
                     /locus_tag="IVK78_002418"
     CDS             7733..8668
                     /locus_tag="IVK78_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375853.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8661..9431
                     /locus_tag="IVK78_002419"
     CDS             8661..9431
                     /locus_tag="IVK78_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6375854.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3056 a         1584 c         2040 g         2875 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
     1561 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
     1621 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
     1681 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
     1741 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
     1801 tacaaggacg tctgatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
     1861 tagcactgtt tggtgcgctc tttgattggc agacaactca accacgtctg cgagacagtc
     1921 tgatttttct tgaaattcaa ttggaaaatt tacactttct ttatacattt ctggctgctt
     1981 tctttatacc cgtcaactat acttcactta tcaataagaa agaaggataa gtatggattt
     2041 tggaaatgaa acggtcttga aagcaactgg tataaccaaa aaatacggtg ctgccaaagc
     2101 attagataaa gtatcgatcg aaatcaaacg tggaatgatc tatggtttga ttggggagaa
     2161 tggagcgggt aagtctacct ttatgagaac tattatgggt ttgatctcga ttgatgaagg
     2221 ttcgatcgaa ttatttggaa cgacggattt gcaggcagca agacgacgta tgggtcaatc
     2281 gattgaaacc cctgctcttt atccagaatt aactgcaaga gataacctac gtatccaagc
     2341 agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg ctgaaaatga tgcgattgga
     2401 aaatacaggg aaaaagaaag caaagaattt ctctttaggt atgcgccagc gtttagctat
     2461 tgcaaatgca ctgatcacga atccagaatt tttgattttg gatgaaccga cgaatgggat
     2521 ggatccagca gggatggcag aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg
     2581 catcaccgtc ttgctttcta gccacttgct agatgaattg tcacagatcg ccactcacta
     2641 cggtattttg catgaaggac acttgatcaa agaattatcg aaagaagaat tggcacaaga
     2701 atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa caagcagtaa cggttctcga
     2761 ctctcttggt tatagggatt attttgtcca atctagccgt gttatccagc tttttgaagg
     2821 aattgaccaa gtagcagcca tcaatcaagc attggtagag gcaaaagttc ctgtggatgg
     2881 tatccaccta gttgggcaaa aactggaaga ttatttctta caattaactg gaggcaatcc
     2941 acatgtttaa tctattaact gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg
     3001 tctcactagg actgatggtc gtacttccgc ttttttattc ttggaacgaa tggtatatgc
     3061 atacaaaata cggcaatgaa ttaaaccaag caacggatac agtcatcaac ggtgcaacag
     3121 ggattttaat ggtggaaaaa atggctgcat ggattcttct ggctttcgca gcatttgcgt
     3181 gcttttatat tggggaagaa tttcaaaacg gtacgattcg caatacgtta tctttaggac
     3241 ggaatcgaat gacttattat gtgtcgaaac ttctcgttac gcttttgatc acaacacttg
     3301 gcgtggtttt gattacgggg ctagccatga ttgcttacac acttgctttc ggatttggtg
     3361 aagttgaggg aatcaaaaac tatggaaatt atgttctaaa agtatttcct gtattgcttc
     3421 tgctgatatt agcgacatta tctgttccag tggctttgac attcatcact agaagtacca
     3481 gtgtcagttt gcttctttct tttctttata tcatgggaac ggccttcgtt cccggtgttt
     3541 tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt tacggaaacg tggctcatgt
     3601 atacggactt tgcgcaacaa gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc
     3661 tagtaacaat cgtggtatca tcagctctag gaatgtttat tttccaaaaa tctgacatta
     3721 agtaggtgtt cctgctggaa actattttga ttatcgaaga cgatgaagct gttcattctt
     3781 tactggagga ggttcttgaa cagagataca aacttcttga tgcttattca ggtacggaag
     3841 gaaaattatt gttaagcaca tatcctgtcg atttgatcct tctagatctc atgcttccag
     3901 ggctttcagg tgaagcattg ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg
     3961 ttctttcagc aaaaagcgat caaagagata aagtttcctt gttggcagca ggagcggatg
     4021 actatgtgac gaaaccattt gatatcgaag aactgttgtt acgaatcggc atccaacttc
     4081 gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg catctattac aaggaaatct
     4141 tactggacaa agagtcgagg gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa
     4201 gagaattcga tctgctgaag ctttttttag aacatccgaa aaaagtattt tcgcgagcaa
     4261 atctttatga aacagtttgg caagaaccat attttgatag tgagaaaaca gtgaatatgc
     4321 atatttccaa tcttcgaggg aaactagcaa aggcacatgc tgtgtatatt catacggtgt
     4381 ggggagtggg gtttagattt gattaagtgg ctgttgcttc tcgttgttat ctttatggga
     4441 agttttattt tcttcctgca aagggaaatc aagagattaa ctaagaaaat cgccactctt
     4501 tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg
     4561 gacctagtag atgcactgaa tacatgggtg gatgaaaatg aacaaaaagg acaagcattt
     4621 caagaaatgg aagaaaacat cagactatcg atcgtcggcc tttctcatga tctcagaact
     4681 cctttgactg ctattaacgg ttatgtacaa ttactggaac aggtagagga tacggagaaa
     4741 cgggagaagt atttaacgat catcaaacaa tctgttgcaa gattactcag tatgacggat
     4801 cagttttatg atcttgctcg aatagagacg aagcaaaaag aaatggatct ccatacgctt
     4861 tctttttcta aagaggtgga agaagtattt ttctcttttt atgaacaatt ttctgagaaa
     4921 aatatcgagg tagcgttcgc tgaacactcg ataaatacaa ctgtattagc agatccgctc
     4981 atgttgctgc gagtgctgca aaatatcata cagaatttat tgcgttatgc agatggacaa
     5041 gcaaaggtga cattttcaaa agaggattca ttcattcgat taacagtggc caatcctatc
     5101 aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc
     5161 agccgcacaa atacggaatc tagcggaatc gggctttacc tgtcaaaaaa attggtggaa
     5221 ggaatgggag gagagatgac cgctagatta gacgggggca tcttctccat ctccgtcaag
     5281 ttgcggaggg tttaattggc tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg
     5341 ttttaaagac atcttctttt ttggaaggtg tttttatttt ggtgtgattg atacgaatta
     5401 ggtaacgaag acagttattt ttgtaccccc ttttcaaata gcgcaacagt gctatactaa
     5461 aactaacaaa tcaaggaacg gagagggata tgggccaaag agaatttatc gttttaatta
     5521 ttattgcagt tgttatttta ttagttgttt tagacatttt ttcacgtttg aaagtaaagg
     5581 aaacagttcg tagtaattgg ggaaaaattc cttatcaacc gagatttgat aaagaagaaa
     5641 gtttgaagga tgcgtggctc acggagaaaa aattccgttc atgggatagt gaaatcgacg
     5701 atcttacttg gtatgacttg gatatgttcg aagtatttga agggatcaac agtacctatt
     5761 ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag ctttgatttt ggagaagatt
     5821 atcagttaga aaaactaatt gctttttatc aggaaaatcc tcaactgaga gaaagaatcc
     5881 aatatcaatt cgctcgttta ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg
     5941 atggaaaaag taaaaagatt ggacgcgtag gtttatttat ttttcttggc ttgcttccgc
     6001 tggtgggtgt aggtttgctt cttttagggc aagtactagg aatctatcta gcaatcggtg
     6061 gcttagtatt caatacggtc tattatctag tgaagaaaca aacactagaa acagagctga
     6121 atagcatgag ttatttggtt tcaacgattt cagccgccaa tcagttagca aaaatgacga
     6181 caccacttca aaaagagctg aaacagcaaa ctaaaccatt aagagacatc cctaaattag
     6241 gtttttcatt tcgaatgaag agcgggagtg aagcagaatt gatttttgaa tatgtcaaca
     6301 tcatgttcat gctgccattt atctcctatc atttcgttct cactaaatta gaaaagcatt
     6361 caactgaagc catcaagctg tgggaattgc tgggagaatt ggaagttgca gcagccgttt
     6421 tgaattatcg cacctatatg ccgatcactt gccgaccaga gtttactgag ggcggtgtga
     6481 cagcgaaaga tatctatcat ccattgttaa aagaagcggt agttaatcca gtcaactggc
     6541 agaagaatac attagtcacg ggttccaatg catcaggaaa atctacgtat gtcaaaagta
     6601 tagccatcag ctgtattcta gcacagacga tccaaacagc gattgcagaa cagtttaccc
     6661 tgcaacctgg acatgtgttg acttccatgg caatcgaaga tgatttattt gaaggcgaca
     6721 gttattttgt ttcagaaatc aaatcgatca aacgtttgct ggaccaagtg gcttccaaag
     6781 aacgctgcta ctgcttcgta gatgagattt taaaaggaac aaatacgatc gaacggattg
     6841 cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc atctttagca tttgtcgcca
     6901 cacatgatat cgaattgaca gagattttaa agaattattg tgagaatgtc cactttgaag
     6961 aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa
     7021 ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta tcctaaatcc atagttgaac
     7081 aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca gtggtatccg tttgattaac
     7141 tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg
     7201 atgccaaaaa agtggcagaa ttcaatggga acgagtctgc tgtccagatg atcttggaag
     7261 atttagcaaa agacgataca aaataaacca cgctgtcttc cttctagcag cttttatcga
     7321 aaaaacttag catcagcgac gagacgcacg ttccatggca aagttctccg taaaacaaac
     7381 agcgaaaaca gaaattgaaa ataactattt ccattttatt tatttcggtt aaaataaaag
     7441 aaaaaagctt tcgataaaag gacggaggaa agcttaacat gaataaaaaa gacaagaacc
     7501 tcagttataa ggatcttcta tatgtcatcg tagtagttgc tgtcggtact tttatgattt
     7561 taaaagttcc agagttgttt agatagttat tctatcgaag atgatcatga actagaaaac
     7621 acatctcctc tttgttagca ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg
     7681 ctgtattcca tgatacaatt aagaatcatg tctataagga gataggaaat aaatggcaaa
     7741 tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg
     7801 aatcgatctt tcggtgaaag aaggagattt ctatgcgcta ttagggccaa atggagctgg
     7861 gaagtctaca acgatcggga ttattacttc tttagtcaat aaaacatctg gaaaagtgaa
     7921 tgtcttcggt tatgatctgg acaaggatct agttcgagca aagcaacaga ttggattggt
     7981 tccacaagag ttcaatttta acccttttga aacggtccag caaatcgtcg tgaatcaagc
     8041 aggctactac ggtgtctctc gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca
     8101 gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg
     8161 aagactgatg attgctaggg cattgatgca tgaaccacgt ttgctgatct tggatgaacc
     8221 aacagcagga gttgacatcg agttgagacg cgagatgtgg gagtttctac gagaattgaa
     8281 cgaaaatggt acaacgatca ttttgacgac gcattatcta gaagaagctg aaatgctttg
     8341 tcgcaacatc ggaatcatcc aatcgggtga actgatcgag aatacaagta tgaaagagct
     8401 tctgtcaaaa ctgcaatttg aaacatttat ctttgattta gcgccatatg ataccaaacc
     8461 tgtgattgaa ggatatgctt gtacatttga agatgagcag acaatcgctg tcgaagttga
     8521 acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca agacaaggaa tcaaagttct
     8581 ttctatgagg aataagtcga atcgtttaga agaactgttc ttgaaaatca cagaagaaaa
     8641 taatcatacg gaggagaaaa atgtttaatc tttattttac tgcattaaaa agtttagcta
     8701 tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac actggttccg ccagtgatca
     8761 cgacatcctt gtattttgtt attttcggaa aaatgatcgg tgggcggatc ggtgacatgg
     8821 gtggtttttc atatatggaa ttcatcgttc ctggtttgat tatgatgtct gctatcacta
     8881 gttcgtatgc caatgtttct tcgtccttct tctctcaaaa atttcagaaa aatatcgaag
     8941 aaatcttggt agcacctgtt cctacgcatg tgattatttg gggctttgtg attggtggtg
     9001 tgggtcgcag tattctcgtc ggctctctcg taacgatcat ttccttgttc tttgtaccac
     9061 ttcatgtgta ttcatggttc atggtcatca ttacattatt gatgacggca atcttgtttt
     9121 cattagcagg actgctcaac gggatttttg ctcagtctta tgatgatgta tcgatcgtgc
     9181 ctacatttgt cttgcagccg ttaacttatc taggtggcgt tttctatgcc atctctatgt
     9241 taccgccctt ttggcaagct gtttcaaaag tcaatccgat cgtttatatg atctccggtt
     9301 tccgttacgg attccttgga acgatcgatg tgccagtagc aacatcgatg atcgtacttg
     9361 tgttattcat cgttgttttg tacgccgtta cttggtattt gatcaataaa ggaagaggat
     9421 tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
     9481 cgggaaaata gttcctcata ttttgttgtt ttttgagcga ataccgaggg tcagctgatt
     9541 gaacaccgtt tagtg
//
LOCUS       DACCCM010000093         9422 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCCM010000093 DACCCM010000000
VERSION     DACCCM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.097"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..465
                     /locus_tag="IVK78_002420"
     CDS             4..465
                     /locus_tag="IVK78_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6375855.1"
                     /translation="MTHSHKLMFEDRTIPLESYPTEQIIAEKLSATFDFLTDNSRGKD
                     FYDLYTIPKMEKIDEKTLKDSVRNTFTRRGKTDPLKIYYEKDMDVLKNSHELKDTWKK
                     YQKSNPYAASITYEETMDSISNLMEKVIHVEEKERKLAYQRHQQMNREMER"
     gene            468..941
                     /gene="ssb"
                     /locus_tag="IVK78_002421"
     CDS             468..941
                     /gene="ssb"
                     /locus_tag="IVK78_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6375856.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            1307..1567
                     /locus_tag="IVK78_002422"
     CDS             1307..1567
                     /locus_tag="IVK78_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375857.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            2012..2218
                     /locus_tag="IVK78_002423"
     CDS             2012..2218
                     /locus_tag="IVK78_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6375858.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            2218..2469
                     /locus_tag="IVK78_002424"
     CDS             2218..2469
                     /locus_tag="IVK78_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375859.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            2485..2901
                     /locus_tag="IVK78_002425"
     CDS             2485..2901
                     /locus_tag="IVK78_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375860.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            2883..3518
                     /locus_tag="IVK78_002426"
     CDS             2883..3518
                     /locus_tag="IVK78_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375861.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFISYTICRKHYYSES
                     EYYSIFESMELMEELNSKDATVDDYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLRE
                     YVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKQMPYQRFERKKGQ"
     gene            3899..4078
                     /locus_tag="IVK78_002427"
     CDS             3899..4078
                     /locus_tag="IVK78_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375862.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            4488..4889
                     /gene="tnpA"
                     /locus_tag="IVK78_002428"
     CDS             4488..4889
                     /gene="tnpA"
                     /locus_tag="IVK78_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6375863.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            4901..6046
                     /gene="tnpB"
                     /locus_tag="IVK78_002429"
     CDS             4901..6046
                     /gene="tnpB"
                     /locus_tag="IVK78_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6375864.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            6284..6547
                     /locus_tag="IVK78_002430"
     CDS             6284..6547
                     /locus_tag="IVK78_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP6375865.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            6541..6891
                     /locus_tag="IVK78_002431"
     CDS             6541..6891
                     /locus_tag="IVK78_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6375866.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(6915..7529)
                     /locus_tag="IVK78_002432"
     CDS             complement(6915..7529)
                     /locus_tag="IVK78_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6375867.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            7843..8265
                     /locus_tag="IVK78_002433"
     CDS             7843..8265
                     /locus_tag="IVK78_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375868.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            8275..8478
                     /locus_tag="IVK78_002434"
     CDS             8275..8478
                     /locus_tag="IVK78_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375869.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            8689..9273
                     /locus_tag="IVK78_002435"
     CDS             8689..9273
                     /locus_tag="IVK78_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6375870.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         3355 a         1487 c         1769 g         2811 t
ORIGIN      
        1 acgatcactc atagtcataa attgatgttt gaagatcgaa cgattccttt ggaatcttat
       61 cctacagaac aaattattgc cgaaaaatta agtgcaacct ttgatttttt aacagataat
      121 tcgagaggaa aagattttta tgatctctat actattccta aaatggagaa gatcgatgag
      181 aagaccttga aagatagtgt aagaaataca tttacacgtc gaggaaaaac tgatccgtta
      241 aagatttact atgaaaagga tatggatgta ctgaaaaata gtcacgaact aaaagatacc
      301 tggaagaaat atcaaaaaag caatccttat gctgcgtcga tcacgtacga agagacaatg
      361 gattcaatta gtaatttgat ggaaaaagtg attcacgtcg aagaaaaaga gcgaaaacta
      421 gcttatcaaa gacaccaaca gatgaataga gaaatggaga gataaagatg attaataatg
      481 tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca agtggcacag
      541 cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac ggcaatcgag
      601 aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg gcgacattag
      661 ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact tatgacaacc
      721 agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa ctattagaaa
      781 gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt ccaagcccca
      841 ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat ccatttggtg
      901 gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt tctattggtt
      961 gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa tctataacca
     1021 aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa gaattaacgg
     1081 gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg
     1141 gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc
     1201 tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat
     1261 attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt
     1321 tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt
     1381 acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt
     1441 gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa
     1501 ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc gattgatggg
     1561 catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt tgttttacat
     1621 agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct caattgaaaa
     1681 acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gttgcacgtt
     1741 cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct ctcaccaaaa
     1801 ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa gttgcctttt
     1861 atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtccttgtcg
     1921 cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg aaaggaagta
     1981 aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt attggggtgt
     2041 tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat gcgattgtgg
     2101 agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat gcgggaaaaa
     2161 atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga aacgttgatg
     2221 aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt ggacaaagaa
     2281 gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac gacagaaacc
     2341 cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat tgcaggatat
     2401 gaactgcatt atcaagaaaa cttggaaact tatgaaaacg aatattggtt aaccgaatta
     2461 gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt tgtcaaatta
     2521 cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa tgagacacgt
     2581 gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat gattgaatca
     2641 ggtgtcgaaa tgaatctcat tgcgacgtat tatcgaacac tggaagaact gaaaaagcaa
     2701 aatgcaaaat ggtttttcca agccttacta tgcttggaag taggcgtcaa accaagcaca
     2761 atcaaacctt ctgaatacca agctttagaa ctaacgtatg cgaaatttat cgaaacgaaa
     2821 aaagctaaga ctgtttcaag tgaatggtta gattattttg aaaatatcaa taagtatggc
     2881 gcatgttaca tcatgaagta atcagacgga ttcaaaaaga ctttagttgt atggaagaaa
     2941 tgaagtactt tgttcaaaat gttgaagaac agttatattt ggaaagcgaa acgagtcaaa
     3001 caaaggtcac agtcagccat aatcgtttag atgtgtataa acgtcgtcct gaatcattaa
     3061 aaaaaggagc ggagcttcta cgactagaaa cagaaaaata cgtcatgaat gtttacctag
     3121 aaaaaagtga gttggtcgaa aattttatta gctacacgat ttgcaggaaa cactattact
     3181 cagaatcaga atattattca atttttgaga gcatggaatt aatggaagag ttaaacagta
     3241 aagatgcgac ggtagacgat tattaccttg tatttactag atttttaacg tgtggaacgg
     3301 aatctatcgt gctaatggga atggattacg aactcgctgt acccgaagcg tttttgaggg
     3361 aatatgtaga agaagtagat aacccagggg atgattggta cacgacttta actgatttct
     3421 tagacaccta cacttacgac gatacagaag aaatccaaaa tgagcttgac cgtaaacaaa
     3481 tgccatatca acgatttgaa aggaaaaaag gtcaataatc atttataccg aaaagtgaga
     3541 atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa
     3601 tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgggcaaag cccgataacc
     3661 taagagaaag aaggagatcc atatgaataa aatcattcct ctgttgttcg tactgtgtat
     3721 tggcgagata cttatgcgtc atggtgcagc tgtacattta aacttcgtag tattcttagg
     3781 aatactgata ttttgtttga tcttttggcg ctttgtgtta gcccttgtga gtctaatatt
     3841 caagttgatt gcagccatat ttttcatagg gttatttcga tgaaaggaga gagtactaat
     3901 gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga gacaaaaaga
     3961 attgcagcaa ctaaagcaga agtacggaaa aagtgaatct aagattatgt gtattgcgtt
     4021 agatctattg atcgcgcaag aaaaagcagg atttgatata ccagcactaa aaaagtgacg
     4081 tcacttttca tttgaagaaa caaaagtgat agcacttaat tgttcctact atttagaata
     4141 ttttctatgt cctttttatt ccattttgta tcaataacta tgaaatcttt aaagtatcca
     4201 gaagagatta tttttagatc aatttttttc ttacctgatt ctaaagattt tgttttctta
     4261 aacatattat gatttccttt cgactatttt catataaatg ttaacatagt ttatggacta
     4321 gccgagaaca cccgtgattt taatcatggg atgaatcgga ttcagttgtg acctagcttt
     4381 agctaggtta aacaactgac aaatataaac taaataatag tgaaatgtct tgcaataatt
     4441 gattttgtag aaatacatat attaaaataa ttgcgaggtg tttttgtatg tcagaaattc
     4501 attatggacg gggttatgtc tattctattc aatatcatct tgtttggtgt gtaaaatatc
     4561 gacatgatgt tttgtatggt caggtagata ttgatgtcaa acagtttttg aaacaaattg
     4621 ctgatgataa taatataaaa attatagaaa tagaatcaga taaagaccat attcatttac
     4681 tcattgaatg caccccacaa cactatattc ctagtattgt taaagtattt aaaggcgcat
     4741 ccgctagact gctgtttaaa aaacatcctg aacttaaaca actcctttgg ggtggacatc
     4801 tttggaatcc aagctacttt gtagctactg ttagcgaaaa tacagaagaa caaattagaa
     4861 aatatataca aaatcagaaa aagaaatgag gtgaatccgt atgctacaac atagggctta
     4921 taaatttaga atctatccta atcaagaaca agaaatatta atgactaaaa ctattggctg
     4981 ttcaagattt gtctataatt attttttgaa tttgtggaat catgaatata cgactactgg
     5041 aaaaggatta tcttataatt cttgttctgc catgcttcca caaatgaaaa gaaatgcaga
     5101 gacaatttgg ttaaaagaag ttgacagtat tgcgattcaa tcctcattga aaaatctttc
     5161 tgatgctttt tctcgcttct ttaaaaagca aaataaacga ccacaattta aaagtaaaaa
     5221 acatcctata caaagttata caactaaaaa tgtgaataat agtatcgaaa ttagaaacaa
     5281 tttcgtaaaa ctacctaagt taggatttgt taaatttgcg aaaagtcaag agcccaaagg
     5341 gcgaatctta aatgctacga ttcgaaaaaa ttcgagtggt aaattttttg tttccatcct
     5401 gtgtgaagaa gaaatagttg agtttcctaa atctaattcc gctattggaa tcgaccttgg
     5461 tataactgat tttgcaatcc tttctgatgg tcaaaagatt gataacaata aattcacgtc
     5521 aaaaatggca aaaaaactca aacgtgaaca acgtaagttg tcaaaacgtg cattactagc
     5581 taaaaagaaa ggcgtcaatc tctttgaagc taaaaattat caaaaacaaa aacgaaaagt
     5641 agcacgacta catgaaaaag taatgaatca acgcaatgac tttttaaaca agttgagcac
     5701 agagattatc aaaaaccacg atattatctg tatagaagac ttaaatacaa aaggtatgtt
     5761 gcgtaatcat aaactagcaa aaagcatttc tgatgtgtct tggtctagtt ttgtgactaa
     5821 gttacaatac aaagctaact ggtacggtcg gaaaatcatc aaagtagata aatggtttcc
     5881 atctagtcaa atctgttcaa attgtggaca caaagacggc aagaaatctc tagaaattcg
     5941 agaatggact tgtcctgttt gtcatgctca tcatgaccga gatatcaatg ctagtatcaa
     6001 tattttgacc gaaggtctaa gattacatgt catgggatta gcttagatcc aatcatcaaa
     6061 aaccgtagga actacgggga tagcttggta aataagagaa accgctgtga gtaaagaaat
     6121 acgcttgcaa gtatgctcta ttcccaagaa tctcgtgact ttagtcatga gaggttcaag
     6181 gattcatgga atgttaatct aatttaatag aaaaggataa ttaagtatat acgttttttc
     6241 ttatgtatgt tatactatgt atatacagaa agaggtgtct actatggaaa tgttacctat
     6301 aaaaaaatgg ggaaatagta atggccttag attgccaaag tatattatgg aatatcttgg
     6361 aattcataca gaagacaaag tgaagataat acaagaagaa gtaaacggtc agaagcggct
     6421 aattatcgaa gcggctaata tagaaaatga attaacaatt gaacagttat ttgaaaatta
     6481 cactgatgaa agaaatcatg tgacgattca aaatttaggt gaagcagtag gtaatgaaaa
     6541 atggtgagaa aacctagaca aggcgatatt ctattattga atacagcacc tcgatctgga
     6601 cacgaacaga caggtaaaag accttatatt gttttaagtc atgatattat tgcggattat
     6661 agtaacgttg ttacagttgc tccaatttct agtacaacta gaaactatcc gctatatgta
     6721 aatataaact cagaatatga aatgaagaca acaggaaaag tattattaga tcaactaacg
     6781 acaattgatt atgaagccag agagtgtatt tttttggaaa aagcgcatga tagtttagtt
     6841 gaggagttat tgatgaaagt tagaacagtt tttcaaaaag taagcaaata aaatagaaac
     6901 agcagcatga tcaactattc gtgattgacc atgctgtctt tttttattct atatacggtt
     6961 tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg caacaccttt ttttagatcc
     7021 tcaacgatgt ttttataaac aagacgacgt tggggatctt ttgcattggc actgtatagc
     7081 ttaggacgtc ctttataaac accgttcgct tttgcaatct taatgccttg tgcctgacgt
     7141 cgtttagatt cggtacgttc ttgttcagcg atcatggcca aaatttgaat gatcaaatct
     7201 ttaatgaatt tatccaggag aggattgcca attgcttcgg ccatgatcgg tagacttgtg
     7261 atgatcagct gcacattttt cttcttcaag taattgactg aagcgatgat ctcgtcgtaa
     7321 tttcttccaa gtcgatcaat agcttccaca ataaaaatat cttgatctcg gacgaaatcc
     7381 agtgcttctt gaaaaacagg tcgatgagta atcgttgcgc cagattgttt ctcaatgaaa
     7441 attctctctg caccgaattt tttcatttct tcaatctgcc gttgttcatt ttgatcgatg
     7501 gatgagacac gtatgtatgc aactctcaaa atttttacct actttctgca actatttaaa
     7561 aaacacccta atcatacacg agaaaaccag atttttcaat gtcgcggtcg tgtctagtta
     7621 gggtatacct tatatagaca aaaactaatg atggaggaaa tagtatgtgt cacttttctt
     7681 tttaaatggc aatatttttt gtaaataaat gtcaatcaag aaaatttaaa cgattatgac
     7741 attaaaaata tattttatga attttctagt atataacttg atttatctgt taagatttat
     7801 taagaaaacg ttttcttatt tttgatttcg aggtgataga caatgaaaaa taagtttatt
     7861 ttatctttag gtattttaac tattggaatt agtgtaactg gaactacaat tcctacattt
     7921 gctgatagta caaatgaacc aactactgta gtagataata accaagaatt aactccttta
     7981 gataggaacg gaatagccat tggtaaccca actaacgact taactgttac tgaaaattat
     8041 ttaatagttt ctccattact tgctcgagca gcttctaaaa cgatacgagt gacaggaacg
     8101 atgatttttg ataaagttgg ttggccttct agacctcctc aatcacggaa ttggaaagaa
     8161 aaaagaaatg gttattggta ctatggaaaa ttgtatttac aaagttataa atcagctgga
     8221 aacaattgga ttgcgactta ttcaggaacc ttaactagaa agtagggatt ttttatgcca
     8281 gaatttgtga acacaccaaa tttttatcaa gatagcgata ttctaagtaa aattaattta
     8341 ccgaaagaag aatcagcttt acaagttcag cgaactttac aggagacaca aaaaattcaa
     8401 aaatcaattt ctaatctaga agattctgaa gaaaaaagaa aaaaaagaga ccaaaaaaca
     8461 tatcaaaaag aaaaataatg attttaaaaa aagagtgtct agtcgacatt ctttttttct
     8521 aattgaaagt gttcataaaa tcacccattt ctggacactt tctttttttg ctttattttt
     8581 caaaaaaaca attcatttca tgttcataaa cccataacaa tatctatgtt cagttaaact
     8641 atatggataa agttatgtta caataaaatt aatgaaggag tgattttttt gaaaatagga
     8701 tacgcccggg tatctactgg tttacaaaat ttggatttac aagaagacag cctaaataaa
     8761 tttggttgtg aaaaaatttt tactgatcat atgagtgggg caaaaagtaa tcgaccagga
     8821 ttagaaatgg ccattgattt tgttcgctct ggcgatacac ttgtcgtttg gcgcttagat
     8881 cgtttaggta gaaatatgga agatttgatt tcaatcgtca atcgactgaa tgagcgtggt
     8941 gtgagttttc atagtattca agaaaatatt acgatggaca aatcaagttc tacaggacag
     9001 ctcatgtttc atttgtttgc ggcatttgca gaatttgagc gtaatttgat cctggaacga
     9061 tccgcagcag gacgagcagc tgctcgtgca agaggacggt ttggtggtcg tcctgaaaaa
     9121 ttgtctgaac aagatttgga attactgaag actttagtgg atagcggtac acctattaag
     9181 actattgcgg aacggtggaa cgtctcaaga accaccattt atcgttattt ggcaaaaatg
     9241 gaggagaaaa atggaaagac aaattctcat tagtttttgt catttaatgc tgaaaatttt
     9301 tgccatttat ctagaaaagt gacaatagta tgagaatttt ttgtaagtgt ctactgataa
     9361 cttattctca aatatttagc tatttcttct ttacaacatc gaacatatgt ttgtataatg
     9421 tg
//
LOCUS       DACCCM010000094         9388 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCCM010000094 DACCCM010000000
VERSION     DACCCM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.098"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            2..77
                     /locus_tag="IVK78_002436"
     tRNA            2..77
                     /locus_tag="IVK78_002436"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            79..149
                     /locus_tag="IVK78_002437"
     tRNA            79..149
                     /locus_tag="IVK78_002437"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Gly,seq:tcc)
     gene            163..236
                     /locus_tag="IVK78_002438"
     tRNA            163..236
                     /locus_tag="IVK78_002438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ile,seq:gat)
     gene            261..348
                     /locus_tag="IVK78_002439"
     tRNA            261..348
                     /locus_tag="IVK78_002439"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Ser,seq:gct)
     gene            370..441
                     /locus_tag="IVK78_002440"
     tRNA            370..441
                     /locus_tag="IVK78_002440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Glu,seq:ttc)
     gene            464..553
                     /locus_tag="IVK78_002441"
     tRNA            464..553
                     /locus_tag="IVK78_002441"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ser,seq:tga)
     gene            566..639
                     /locus_tag="IVK78_002442"
     tRNA            566..639
                     /locus_tag="IVK78_002442"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Met,seq:cat)
     gene            658..730
                     /locus_tag="IVK78_002443"
     tRNA            658..730
                     /locus_tag="IVK78_002443"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Asp,seq:gtc)
     gene            734..806
                     /locus_tag="IVK78_002444"
     tRNA            734..806
                     /locus_tag="IVK78_002444"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Phe,seq:gaa)
     gene            811..881
                     /locus_tag="IVK78_002445"
     tRNA            811..881
                     /locus_tag="IVK78_002445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            894..967
                     /locus_tag="IVK78_002446"
     tRNA            894..967
                     /locus_tag="IVK78_002446"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Ile,seq:gat)
     gene            979..1050
                     /locus_tag="IVK78_002447"
     tRNA            979..1050
                     /locus_tag="IVK78_002447"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Glu,seq:ttc)
     gene            1316..2509
                     /locus_tag="IVK78_002448"
     CDS             1316..2509
                     /locus_tag="IVK78_002448"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP6375871.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2639..4111
                     /locus_tag="IVK78_002449"
     CDS             2639..4111
                     /locus_tag="IVK78_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6375872.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4194..4982
                     /locus_tag="IVK78_002450"
     CDS             4194..4982
                     /locus_tag="IVK78_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP6375873.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5049..5717)
                     /locus_tag="IVK78_002451"
     CDS             complement(5049..5717)
                     /locus_tag="IVK78_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6375874.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5832..7544
                     /locus_tag="IVK78_002452"
     CDS             5832..7544
                     /locus_tag="IVK78_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375875.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7547..9319
                     /locus_tag="IVK78_002453"
     CDS             7547..9319
                     /locus_tag="IVK78_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6375876.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2767 a         1643 c         2122 g         2856 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
      121 tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg
      181 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag
      241 tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag
      301 ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt
      361 gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
      421 gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc
      481 cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat
      541 cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc
      601 ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt
      661 tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc
      721 gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      781 cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc
      841 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta
      901 tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt
      961 aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg
     1021 gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt
     1081 tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct
     1141 aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa
     1201 aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag
     1261 gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt
     1321 agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga
     1381 ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc
     1441 gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc
     1501 atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc
     1561 aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc
     1621 tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt
     1681 attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac
     1741 accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga
     1801 tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt
     1861 tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca
     1921 tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga
     1981 agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg
     2041 atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga
     2101 tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa
     2161 agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg
     2221 gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc
     2281 gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt
     2341 gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc
     2401 gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg
     2461 ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag
     2521 ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa
     2581 aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat
     2641 gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac
     2701 ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg
     2761 aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc
     2821 gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt
     2881 attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc
     2941 tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat
     3001 cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg
     3061 ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg
     3121 gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct
     3181 atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt
     3241 tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg
     3301 aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt
     3361 ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact
     3421 atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt
     3481 ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt
     3541 attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac
     3601 tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc
     3661 ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt
     3721 catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt
     3781 atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca
     3841 aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc
     3901 agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc
     3961 taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca
     4021 ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt
     4081 tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat
     4141 cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat
     4201 ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac
     4261 taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac
     4321 ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt
     4381 cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg
     4441 gctggtacac agatacagtt tatgacatga agtattggac attcattgca gaagagctac
     4501 ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg
     4561 ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt
     4621 tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt
     4681 tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag
     4741 gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc
     4801 caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg
     4861 tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata
     4921 actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat
     4981 aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag
     5041 gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag
     5101 gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt
     5161 tctttaaatc cctctaattg attttcaact acataattac gcataaatag aactgtttct
     5221 ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc
     5281 attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact
     5341 gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga
     5401 aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca
     5461 tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc
     5521 gctaattctt cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa
     5581 taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc
     5641 tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga
     5701 gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa
     5761 attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt
     5821 gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta
     5881 tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac
     5941 agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt
     6001 acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg
     6061 caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga
     6121 cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa
     6181 atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc
     6241 cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga
     6301 tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg
     6361 gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa
     6421 acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat
     6481 tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg
     6541 tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg
     6601 tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact
     6661 acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa
     6721 gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt
     6781 atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc
     6841 gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag
     6901 aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc
     6961 ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag
     7021 taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct
     7081 caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg
     7141 aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg
     7201 aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca
     7261 ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac
     7321 tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa
     7381 cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat
     7441 tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg
     7501 tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat
     7561 aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat
     7621 ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga
     7681 agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag
     7741 caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa
     7801 ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat
     7861 cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg
     7921 tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc
     7981 tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg
     8041 ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc
     8101 tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa
     8161 actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt
     8221 acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt
     8281 caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa
     8341 tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc
     8401 ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact
     8461 gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt
     8521 gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg
     8581 tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt
     8641 cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac
     8701 aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg
     8761 ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca
     8821 tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac
     8881 attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat
     8941 tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa
     9001 cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc
     9061 atctttattg attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat
     9121 ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct
     9181 gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga
     9241 aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca
     9301 atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt
     9361 ttatgttgtt tgtttttata aggggcag
//
LOCUS       DACCCM010000095         9154 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCCM010000095 DACCCM010000000
VERSION     DACCCM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.099"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            806..1006
                     /locus_tag="IVK78_002454"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IVK78_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IVK78_002455"
     CDS             1493..1672
                     /locus_tag="IVK78_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375877.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IVK78_002456"
     CDS             complement(1718..2323)
                     /locus_tag="IVK78_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6375878.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IVK78_002457"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IVK78_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IVK78_002458"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IVK78_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IVK78_002459"
     CDS             3358..4329
                     /locus_tag="IVK78_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6375879.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IVK78_002460"
     CDS             4469..5782
                     /locus_tag="IVK78_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6375880.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IVK78_002461"
     CDS             5825..6691
                     /locus_tag="IVK78_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6375881.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IVK78_002462"
     CDS             6688..7536
                     /locus_tag="IVK78_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6375882.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IVK78_002463"
     CDS             7550..9022
                     /locus_tag="IVK78_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP6375883.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9154
                     /locus_tag="IVK78_002464"
     CDS             9039..>9154
                     /locus_tag="IVK78_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6375884.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2920 a         1525 c         1871 g         2838 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caat
//
LOCUS       DACCCM010000096         8969 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCCM010000096 DACCCM010000000
VERSION     DACCCM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.100"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(57..350)
                     /locus_tag="IVK78_002465"
     CDS             complement(57..350)
                     /locus_tag="IVK78_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP6375885.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IVK78_002466"
     CDS             complement(378..1043)
                     /locus_tag="IVK78_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP6375886.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IVK78_002467"
     CDS             complement(1183..1815)
                     /locus_tag="IVK78_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6375887.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IVK78_002468"
     CDS             complement(1822..2889)
                     /locus_tag="IVK78_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6375888.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IVK78_002469"
     CDS             complement(2886..3617)
                     /locus_tag="IVK78_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP6375889.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IVK78_002470"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IVK78_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6375890.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IVK78_002471"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IVK78_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP6375891.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="IVK78_002472"
     CDS             5780..6949
                     /locus_tag="IVK78_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6375892.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7216..8838)
                     /locus_tag="IVK78_002473"
     CDS             complement(7216..8838)
                     /locus_tag="IVK78_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375893.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1701 c         1333 g         3079 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     7381 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     7441 tacaggtaaa actattgttt tgccttttga agatttaagc gtaaatgtaa tttcatcttt
     7501 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
     7561 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
     7621 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
     7681 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
     7741 tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata ctgaaaaaga
     7801 tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat ttaaaggagt
     7861 attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata ttatacccat
     7921 tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta gtataattaa
     7981 acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta tacttccaat
     8041 caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat aaggtctatt
     8101 cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg aaagaagtaa
     8161 agtaactata gcagctttta ttaatttaat aattaagaag atttctattt tctttttccg
     8221 ataaaattga ataattaaag caattataat cattaccata gtcatagcta cagaaagaac
     8281 atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt ctttttttga
     8341 gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg cgagtaattc
     8401 ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg tatatataat
     8461 agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat aatgacatat
     8521 aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg ctaaattagt
     8581 aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat tatttacttg
     8641 catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag attctaaact
     8701 taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca tgtttttaaa
     8761 tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt ttacagaaaa
     8821 aatttttttc aaattcatat accacctcat gtttttttat attacatata gtatcattta
     8881 tttttttaaa acactagatt agtcactata taaaatgtga ttaattttat tattaataaa
     8941 atatttttta tattacaaaa gtagtgttt
//
LOCUS       DACCCM010000097         8661 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCCM010000097 DACCCM010000000
VERSION     DACCCM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.101"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IVK78_002474"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IVK78_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP6375894.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IVK78_002475"
     CDS             complement(738..1460)
                     /locus_tag="IVK78_002475"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP6375895.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IVK78_002476"
     CDS             complement(1596..2477)
                     /locus_tag="IVK78_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP6375896.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IVK78_002477"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IVK78_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP6375897.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IVK78_002478"
     CDS             3717..4196
                     /locus_tag="IVK78_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP6375898.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IVK78_002479"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IVK78_002479"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP6375899.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IVK78_002480"
     CDS             5497..5625
                     /locus_tag="IVK78_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6375900.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IVK78_002481"
     CDS             5629..6174
                     /locus_tag="IVK78_002481"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6375901.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IVK78_002482"
     CDS             complement(6628..8319)
                     /locus_tag="IVK78_002482"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP6375902.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2410 a         1771 c         1568 g         2912 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaaccca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa a
//
LOCUS       DACCCM010000098         8525 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCCM010000098 DACCCM010000000
VERSION     DACCCM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.102"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK78_002483"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK78_002483"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP6375903.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IVK78_002484"
     CDS             complement(1098..2201)
                     /locus_tag="IVK78_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6375904.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IVK78_002485"
     CDS             complement(2291..3226)
                     /locus_tag="IVK78_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP6375905.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IVK78_002486"
     CDS             3528..5462
                     /locus_tag="IVK78_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6375906.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IVK78_002487"
     CDS             5919..6335
                     /locus_tag="IVK78_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP6375907.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IVK78_002488"
     CDS             6349..6915
                     /locus_tag="IVK78_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP6375908.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IVK78_002489"
     CDS             6938..7924
                     /locus_tag="IVK78_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6375909.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8525)
                     /gene="scm"
                     /locus_tag="IVK78_002490"
     CDS             complement(8079..>8525)
                     /gene="scm"
                     /locus_tag="IVK78_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6375910.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSTDTSA
                     SSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTS
                     NDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2508 a         1647 c         1729 g         2641 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt ggtagaggta gtatctgtac tgtcacttga agctgacgtg
     8461 tccgtgctat cacttgttgt ggtagatgta tccgtactgt cgcttgaagc tgacgtgtcc
     8521 gtgct
//
LOCUS       DACCCM010000099         8492 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCCM010000099 DACCCM010000000
VERSION     DACCCM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.103"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..173)
                     /locus_tag="IVK78_002491"
     CDS             complement(<1..173)
                     /locus_tag="IVK78_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6375911.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVK78_002492"
     CDS             complement(593..1714)
                     /locus_tag="IVK78_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP6375912.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVK78_002493"
     CDS             2042..3451
                     /locus_tag="IVK78_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP6375913.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVK78_002494"
     CDS             3448..4176
                     /locus_tag="IVK78_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6375914.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVK78_002495"
     CDS             complement(4304..5215)
                     /locus_tag="IVK78_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6375915.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVK78_002496"
     CDS             complement(5232..6239)
                     /locus_tag="IVK78_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6375916.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVK78_002497"
     CDS             complement(6236..8365)
                     /locus_tag="IVK78_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6375917.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVK78_002498"
     CDS             complement(8370..8477)
                     /locus_tag="IVK78_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375918.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tt
//
LOCUS       DACCCM010000100         8401 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCCM010000100 DACCCM010000000
VERSION     DACCCM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.104"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(401..718)
                     /locus_tag="IVK78_002499"
     CDS             complement(401..718)
                     /locus_tag="IVK78_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6375919.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(719..973)
                     /locus_tag="IVK78_002500"
     CDS             complement(719..973)
                     /locus_tag="IVK78_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6375920.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1332..1736
                     /locus_tag="IVK78_002501"
     CDS             1332..1736
                     /locus_tag="IVK78_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP6375921.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1753..2901
                     /gene="tnpB"
                     /locus_tag="IVK78_002502"
     CDS             1753..2901
                     /gene="tnpB"
                     /locus_tag="IVK78_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6375922.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3124..3342)
                     /locus_tag="IVK78_002503"
                     /pseudo
     CDS             complement(3124..3342)
                     /locus_tag="IVK78_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3608..3802)
                     /locus_tag="IVK78_002504"
     CDS             complement(3608..3802)
                     /locus_tag="IVK78_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375923.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            5154..6194
                     /locus_tag="IVK78_002505"
     CDS             5154..6194
                     /locus_tag="IVK78_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6375924.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            7018..7350
                     /locus_tag="IVK78_002506"
     CDS             7018..7350
                     /locus_tag="IVK78_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6375925.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            7969..8310
                     /locus_tag="IVK78_002507"
     CDS             7969..8310
                     /locus_tag="IVK78_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6375926.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         2977 a         1267 c         1417 g         2740 t
ORIGIN      
        1 ccatagatat attggacttc ctcttttaaa acacaaaaat aaatactacc ttatttaggt
       61 agtatttatt aaaagttacg tcaattttga ttaaaaatat actacttgaa cctctcatga
      121 gtaaagtcac gaaatttttg ggaatagagc gtacttgata tcttattttt tcactaatag
      181 cagtgtctct tattcaccaa gcgatccccg tagttcctac ggttcttaat ttttctctag
      241 actaatgatt gtatctttag accttcggcc ataacatgga gcaaaataaa aaggtactaa
      301 gtcttatata tcaatcttcg agtaaccgac ggttaacaac catatcgagc ggcattaatc
      361 acccacctgt agaggatgag cgactttttc ctaattattg ttaaaagcct agtttcattt
      421 gtttagattg gaaaatacgt cctacaagta tctctttttt ttcatggtcg atccgataaa
      481 ataaggcata gcttttgccg atcacgatcc gatatgttgg gatatccatt ccatagattt
      541 cggaaacttc ctcatgcata gttggaaaat ttttggttag ttctaaagcc ttctggatcg
      601 cacctacaaa tctttttatg ctggctggag aaagagcaag ctcattttcc cattcttcta
      661 ttatcgtttc aaggctggtc tgaaaacttt tagaataaac tagcgtgtac tctttcatct
      721 attcactcca gccgtatttt ccgaaagctt ttaccatttc aatttctgaa actgttaaac
      781 ctttctcaaa gtcttggaaa ctttcttcaa tcaatttgcg atcgtgttct tttgctattt
      841 gagtagtatc taatatgaat aagttgtctt cttgtttggc ttggataatg tctccgcttt
      901 ttaatccaag agagcttgga aggatcactc ccacgctatt gccgatcttt ctaaccttta
      961 gttcttttcc catgttatca cactcctttt agtttttgat gaataaatta taacatgtta
     1021 taacaacaat tgccaacggt gtttcttaag ggattccact ccatattatt attggacaaa
     1081 ataagccact tagaacgctg aaaacatata caaacgatat caaaatacga gtgaactact
     1141 caccatacac ttccgttaac atccatttaa cagaattagg cagaagtcgc ccatcctcta
     1201 taggtgggtg atgaatgccg ttcggtatga gggttaccgt tggtaactcg aagaccgaca
     1261 actaaggcgt attgcctttt tattttgtcc catgttacaa tcagtctata caagaaatga
     1321 gttgatataa catggaatta gatacaaata atcattcagt atttcttctt tattaccacc
     1381 ttgttttagt aacgaaatat cgtagacaag tgattgatga tgaaatatct gattatgcca
     1441 aaactacttt tgaaagaatt tcagaatcgt atcacattac tttagtggag tggaatcatg
     1501 ataaagacca cgttcatatt atgttcaaag ctcaacctaa aacagaattg acaaaattca
     1561 tcaatgccta taaaagtgcc agctcaagat tgataaaacg tgactttcca agagtcaaac
     1621 aatttctttg gaaagaaatg ttctggtcta aaagcttttg tcttctgaca actggtggcg
     1681 caccaattga tgtgattaag aagtatatcc aaaatcaagg aaacaatcat aaatagaaag
     1741 taggtgaact ttatggaaca actaaaagca tacaaattca gaatctatcc aacagaggaa
     1801 caagaaatct tctttgccaa atcttttggt tgtgtccgta aggtctacaa tctaatgctt
     1861 gatgaccgaa aaaaggcgta tgaagaagtt aaaaatgatt cttctaaaaa aatgactttt
     1921 ccgacaccag ctaaatacaa gaaagaattt ccgtttttga aagaaattga tagccttgct
     1981 ttagccaatg cccaacttaa tttagataaa gcatacaaga atttttttcg ggataaatcc
     2041 gttggatttc cacgtttcaa aagtaagaaa aatcctgttc agagttatac aacgaataat
     2101 caaaatggta caattgcttt gattgatagt aaattcatca aagttcctaa attgaagtca
     2161 ttagtaagaa ttaagcttca tagacaacca aaagggatta tcaaatctgc tacaatatca
     2221 cgtcattcta gcggtaaata ctatatttct ttgttgtgta aagaagaaat tatcgaatta
     2281 cctaaaacta attccacaat tggtattgac ttaggtatta tggactttgc gattctttct
     2341 gatggacaaa aaattgataa taataaattc acgtccaaaa tggaaaagaa actaaagcgt
     2401 gaacaacgca aattgtctag gcgtgcttta ttggctaaaa ataaaggtac caatctattt
     2461 gaagctaaaa attatcaaaa acaaaaacgc aaagtagcta gattgcatga aaaagtaatg
     2521 aatcaacgta ctgattttct gaataagttg agtacagaaa tgatcaaaaa tcacgatatt
     2581 atctgtattg aagacttaaa cacaaaaggt atgttgcgaa atcataaatt agctaaaagt
     2641 atttctgatg tttcttggtc aaagtttgtg actaaattac aatacaaggc tgactggtat
     2701 ggacgaaaaa tcatcaaagt agataaatgg tttccgtcta gtcagatttg ttcagagtgc
     2761 ggacataaag atggcaagaa atctcttgaa attcgagaat ggacttgtcc tgtttgtcat
     2821 actcatcatg atcgtgatat caatgctagt atcaatattc tgaccgaagg tctaagacta
     2881 cattctatgg gattggctta gatccaatca tcaagaaccg taggaactac ggggatagct
     2941 tggtaaataa gagacaccgc tgttagtaaa gaaatacgct atcaagtatg ctctattccc
     3001 aagaagctcc cacttcaagc gttaagaacc gtcaggttta gctaagtggt gagtagttca
     3061 cgtaggtatt tttattatat atagatttaa catatgtcgg tcggttgatt gatatagcta
     3121 aagttataac tcaaccgaag ccgattcatc tacacgaata aagttgcgag tgttctcggc
     3181 taattcataa aaattgttat gtgttccata tagcaatact ggaataactt tattttcttg
     3241 aatctcataa gcaactctat acacggtgac cgcatactta aatccattag accaattacc
     3301 tgataaacct ccagtttttt gctcttactt aaatggatca atggcaattt catcacaaac
     3361 ctatagagga tgagtgactt ctgcctaatt acgttaaaaa atattgaaag taactctatg
     3421 tagcaaaaaa tatctagaat ttcatttcct caaaactgat cgatattgta tctaaaaata
     3481 tatactacct aaaataggta gtatatatta aaatatgtgc ttttgttaga aaaatcaata
     3541 cataaattat catatctgta tatgtcattt aggtatttca gttatgttat aatagttttg
     3601 ctgtctgcta ccccggccta atctggcaag gggaggagga gatataatga aatacctttt
     3661 tgattttgtt tttgcaatca agccacttgc agatgtagtg tgcagttacc tctgcaaatg
     3721 gcttgattgc aaaattgcca aaaaagacag taagcctacc cgttaattct ataatataga
     3781 aacgtaaaaa aaaccccatc agcctgcacg ctgatggggt ttggtgatat aatgaaatac
     3841 ctctttgtta ctcataattg taccataatt agtcacgaat gcaaccaata gaaaaaacta
     3901 cttagaacgg tagatcatcg ctaataacaa tgcattgtcc accaaatgga ttttgtgaag
     3961 cattaaatac attgttatta attgtcgctg cttttaacta ggcctctccc cttctattat
     4021 aaaagaagat atatatcatt tcttaattac ctactacctt aaaccaaaat ttaagtacta
     4081 cctaatttaa gtattactta aacaatctaa aaaatagtta tcccaattgt tatgctttta
     4141 taatacgatt atttgtaaca aaatgaatat tatataatta aacactacca tatttaggta
     4201 gtgtttaatt attagcaaaa caaaaaatat ttattctatt ctcttaactt caactaagtt
     4261 atgctgcaca cctttatata aatcatatgc aactcattag ctagacaaac tacaagcatg
     4321 tagttttatc tgttaattat gtactaccta aataaagtag tttataatta gcaagtatat
     4381 atatttaaaa atttaagatt aattttttta tgacttgtac aaatttatct gatattctct
     4441 tattaagaac tacctaattt gggtagttct tattttttac attttaagta gtatttaatt
     4501 gcaatttggt agtatttttt tattatctta ttcttagaac ggtagtactt aatataaata
     4561 taggtagtat ctaatttaag tattaatggt agtatctaaa ttaacaggta gtatgtaact
     4621 actttaaagt aatttttttc agattaacac agtagtatct tattaaataa gttaatttta
     4681 atggtagtac ataattatta tatatatctg acattggtag tacttattgt attttttagt
     4741 agtatataat ctattgtttt ctagcctaaa gccttgtctt ataagaataa tataatatat
     4801 actactgaaa taaggtagta tatattacta gtaatggtag tatgttttat atgcataagg
     4861 tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt taattttaaa
     4921 actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata tttatttaaa
     4981 gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat tttaggtagt
     5041 atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg ctattttcga
     5101 aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct acaatgactg
     5161 attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa tttccaaaag
     5221 tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt gcctatatgc
     5281 ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg
     5341 tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa aaacaaaaag
     5401 cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag cagatgggat
     5461 ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa ttaaaagtct
     5521 cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa aacgttgata
     5581 aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct gaatcccttg
     5641 gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca gagggtatga
     5701 aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat atagacactg
     5761 aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt atgacaaacg
     5821 acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca ttttcttcaa
     5881 gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa aaagctgaaa
     5941 tagaaagtgg tatttcgata gtttttgaag aactcgatag ttattatgtc aatgcagaac
     6001 aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa
     6061 atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg
     6121 ctaaagtatc aagtgaaaaa cgtttgaatt atgaaagctc cccaagcact attacgaaag
     6181 actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt aacattaaaa
     6241 ttaaaaattt tttattattc taattatatt acgaatatta caaaataata gctattttaa
     6301 tagaatttat aagttaaaat attatgataa aaatcacaaa cgtagccttt tacgtcataa
     6361 gtgttaaaag aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct
     6421 tttttttgag ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa
     6481 atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa
     6541 atttaattgt ctgctatgct tgtgatgaat tgattttcgt tctgcacttt tggatgataa
     6601 atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac
     6661 cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg
     6721 ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt
     6781 ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct aagcacaatg
     6841 ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc
     6901 atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt
     6961 gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg
     7021 tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct
     7081 acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag
     7141 aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa
     7201 aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac aatcaaacat
     7261 gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc
     7321 gcaaaaggga tggggcgttt gatcggctga attgatcatt ctcataactt gtattttatc
     7381 gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagag tcactagaaa
     7441 aataaggagt agacttgatc tatcaacaat tcccttcggg gagtgacggg gtaggtttta
     7501 acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga
     7561 aatgaatttg tgtaaataat aagcgcagag taaacaagag ggaatttaga agttagacaa
     7621 gttagaatag cgtctagatt aatcatgagc agctactaag ggatctagac tttgacgcaa
     7681 actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg
     7741 tgtgctgcag tacatgtaga tacttgcgag gacgttgatt attggatcaa tcgagcattt
     7801 accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt
     7861 atttttaagt aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt
     7921 gatataaaac atagagaaaa tgaaatttac gtaatggagg aatgacaaat gaaaaattat
     7981 gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg ttgggattac
     8041 aaaggatttg tgacgtttag aagaggcgga aattgtaaag gattagatgt tttagcatta
     8101 gacgaggatg atttatacga tcaaacattg actgataatc caattggatt cggcttgcta
     8161 cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga taatggagac
     8221 gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag ctttgaaatc
     8281 attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt acagataaag
     8341 gcttagaagg agtcacgatg attaataaaa aggtagtatt accttaggca ttgagtaagg
     8401 a
//
LOCUS       DACCCM010000101         8370 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCCM010000101 DACCCM010000000
VERSION     DACCCM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.105"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVK78_002508"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVK78_002508"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP6375927.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIVAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IVK78_002509"
     CDS             complement(2032..3273)
                     /locus_tag="IVK78_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6375928.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IVK78_002510"
     CDS             complement(3291..4214)
                     /locus_tag="IVK78_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6375929.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IVK78_002511"
     CDS             complement(4435..6927)
                     /locus_tag="IVK78_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP6375930.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IVK78_002512"
     CDS             complement(6934..7401)
                     /locus_tag="IVK78_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP6375931.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IVK78_002513"
     tRNA            complement(7537..7610)
                     /locus_tag="IVK78_002513"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IVK78_002514"
     tRNA            complement(7668..7741)
                     /locus_tag="IVK78_002514"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
     gene            complement(7763..7836)
                     /locus_tag="IVK78_002515"
     tRNA            complement(7763..7836)
                     /locus_tag="IVK78_002515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7800..7802),aa:Arg,seq:acg)
     gene            complement(7847..7932)
                     /locus_tag="IVK78_002516"
     tRNA            complement(7847..7932)
                     /locus_tag="IVK78_002516"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7896..7898),aa:Leu,seq:taa)
     gene            complement(7949..8020)
                     /locus_tag="IVK78_002517"
     tRNA            complement(7949..8020)
                     /locus_tag="IVK78_002517"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7986..7988),aa:Gly,seq:gcc)
     gene            complement(8032..8104)
                     /locus_tag="IVK78_002518"
     tRNA            complement(8032..8104)
                     /locus_tag="IVK78_002518"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8069..8071),aa:Thr,seq:tgt)
     gene            complement(8122..8203)
                     /locus_tag="IVK78_002519"
     tRNA            complement(8122..8203)
                     /locus_tag="IVK78_002519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8167..8169),aa:Leu,seq:tag)
     gene            complement(8211..8283)
                     /locus_tag="IVK78_002520"
     tRNA            complement(8211..8283)
                     /locus_tag="IVK78_002520"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8248..8250),aa:Lys,seq:ttt)
     gene            complement(8287..8359)
                     /locus_tag="IVK78_002521"
     tRNA            complement(8287..8359)
                     /locus_tag="IVK78_002521"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8324..8326),aa:Val,seq:tac)
BASE COUNT         2318 a         1866 c         1391 g         2795 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccacaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac tacgcgccca agaggagtcg aacccctaac cttttgatcc
     7801 gtagtcaaac actctatcca gttgagctat gggcgcatat ttgaaatgcc gaggaccgga
     7861 atcgaaccgg tacggtgatc actcaccgca ggattttaag tcctgtgcgt ctgccagttc
     7921 cgccaccccg gcgcgattgc tttggcaaag cggaaaacgg ggttcgaacc cgcgaccccc
     7981 accttggcaa ggtggtgctc taccactgag ctatttccgc gtgtttaaag atgccggcta
     8041 aaggacttga accctcgacc ctctgattac aaatcagatg ctctaccaac tgagctaagc
     8101 cggcatgtaa aacttaaatt atgcgggtga agggacttga acccccacgc cgtaaggcgc
     8161 tagatcctaa atctagtgcg tctgccaatt ccgccacacc cgcaaaaata tgagccgtac
     8221 agggctcgaa cctgtgaccc tctgattaaa agtcagatgc tctaccaact gagctaacgg
     8281 ctcatatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
     8341 ccagctgagc taaacctcca tggataactt
//
LOCUS       DACCCM010000102         8289 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCCM010000102 DACCCM010000000
VERSION     DACCCM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.106"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(44..1510)
                     /locus_tag="IVK78_002522"
     CDS             complement(44..1510)
                     /locus_tag="IVK78_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP6375932.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1897..2493
                     /locus_tag="IVK78_002523"
     CDS             1897..2493
                     /locus_tag="IVK78_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6375933.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2551..3033)
                     /locus_tag="IVK78_002524"
     CDS             complement(2551..3033)
                     /locus_tag="IVK78_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375934.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3139..3336)
                     /locus_tag="IVK78_002525"
     CDS             complement(3139..3336)
                     /locus_tag="IVK78_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375935.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3631..4389
                     /locus_tag="IVK78_002526"
     CDS             3631..4389
                     /locus_tag="IVK78_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP6375936.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4449..4877
                     /locus_tag="IVK78_002527"
     CDS             4449..4877
                     /locus_tag="IVK78_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375937.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4923..4995
                     /locus_tag="IVK78_002528"
     tRNA            4923..4995
                     /locus_tag="IVK78_002528"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4956..4958,aa:Gln,seq:ctg)
     gene            complement(5145..5516)
                     /locus_tag="IVK78_002529"
     CDS             complement(5145..5516)
                     /locus_tag="IVK78_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6375938.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5731..5916
                     /locus_tag="IVK78_002530"
     CDS             5731..5916
                     /locus_tag="IVK78_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375939.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(5999..6187)
                     /locus_tag="IVK78_002531"
     CDS             complement(5999..6187)
                     /locus_tag="IVK78_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375940.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6211..6474)
                     /locus_tag="IVK78_002532"
     CDS             complement(6211..6474)
                     /locus_tag="IVK78_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375941.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6632..6889
                     /locus_tag="IVK78_002533"
     CDS             6632..6889
                     /locus_tag="IVK78_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP6375942.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6960..7151)
                     /locus_tag="IVK78_002534"
     CDS             complement(6960..7151)
                     /locus_tag="IVK78_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375943.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7292..7612)
                     /locus_tag="IVK78_002535"
     CDS             complement(7292..7612)
                     /locus_tag="IVK78_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375944.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7635..8069)
                     /locus_tag="IVK78_002536"
     CDS             complement(7635..8069)
                     /locus_tag="IVK78_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6375945.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2627 a         1635 c         1314 g         2713 t
ORIGIN      
        1 ggaactgctg caaataagca attcctgttt ttatgttcat tgattaatga atagagccca
       61 ttagtttttt gattggttct ttcacagtaa atagcaagat accaccgacg attgctactg
      121 caccgataat accgaagtaa gcagattctg ttcccggtgt gtagaagcgt gcaatttgtg
      181 cattgattgc ttgtgaagaa gcatcagcta agaaccaaat agcgattgtt tgtgattcaa
      241 atgctcgtgg tgctaatttt gttgtcacag acaaaccaac tggtgaaaga cacaattcag
      301 caataaccac gatgaagaaa cttcccaaca accacaatgg acttactttt ccacttgtac
      361 cataaagcat acccggcaac atcaatagca agtacgataa tcctgcaaat aaaagtccaa
      421 gagagaattt cactactgta gatggttgac gttcaccaag tttcgtccac atcgttacga
      481 atactggtgt cagaataaag atgaaaattg gattcaatga ttgataccag cttgcttgga
      541 tcgttacacc aaatagcgta gattgtgtac gttcactagc aaataaagct aagatagatg
      601 aaccttgttc ttgtaatgac cagaaaatga ttgctgcaag gaataatggg atataagccc
      661 atactcttgg tttttcttca gcagttacat cttttgaagt cagcattttg ataaagtaat
      721 acaacggtaa gataatcccc aatacactga ttgtattgac aaaaaagtca attgtcagat
      781 gaccagttag ttgtgccccg ccgaatactg cagcgataac aacaagtgcg atgatcaagt
      841 tacggatgaa tcctttacgt tcttcagttg tcaaaggatt cggcggtgtt gtacccactc
      901 cgtctaatgt cttgcgtcct tggatgatgt attgtaataa tccaaagaac atcccaatag
      961 cagctaaaga gaaccctaaa tgatagttat attcttgacc gattgtacca acgattagtg
     1021 gtgctaataa tgcccctaag ttgacaccca tgtaaaaaat cgaaaatcct gcatctcttc
     1081 gcaagtcagt ctttgaataa agatgtccga ccatgccaga aacatttggt ttcaaaaagg
     1141 cggttccgat aataatcaat gccatcgaaa caaacaatgt acttacacca aatggtaaag
     1201 ccaagacgat gtggccaagc ataatgaaaa ctccaccgaa gaatacagtg cgttttgctc
     1261 ctaacactcg gtcagatacc catccgccga cgatactaga catgtagatc aaagatccgt
     1321 agatcgacat gatagctaat gctgttgctc gtggtaatcc aagtcccccg ttggcaactg
     1381 tatcgtacat ataatagata agtaatgcac gcataccgta ataactaaat cgttcccaca
     1441 tctcagtgaa gaacaaagtt gacaatccct tcggctgccc gaagaagccc ttgtcttgct
     1501 gtttttccat aatctatacc ccaattcttt aaattttcag aaaatattat acatctttac
     1561 cgaaaaattt aactaaaaaa cttatttaag aaatataaac taattttcta taaaagcaag
     1621 taatttttaa tggcaatatc ataaaaaaaa gaagatagag atttaataat tatatatcag
     1681 ctgttttttt caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa
     1741 aatccttaca taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt
     1801 gagcatatct gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa
     1861 aaaatgcaaa acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg
     1921 aaaaaccgcc gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta
     1981 acaactttga tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca
     2041 cgcgcagtta tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca
     2101 cttcgcccat tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc
     2161 aaaaatggtt atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa
     2221 gaacaatttg aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc
     2281 gcaacagcta atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat
     2341 tacaacccag ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa
     2401 ctacgttcgc aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg
     2461 aacgatgccg atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt
     2521 gtgacaaaca tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc
     2581 tgcctcatcg tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa
     2641 tttcagcatc ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc
     2701 ttttggctga tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa
     2761 gctattcaaa acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa
     2821 aatcattgct gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt
     2881 ataccaaatg actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac
     2941 agtccgataa ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa
     3001 taaaaacaat agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc
     3061 agaaaatagg aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg
     3121 cacgtccaat tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt
     3181 ttcatctaat aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt
     3241 tgtcattttt gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc
     3301 atattgttct cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa
     3361 cttttctcca cattaccact gctatttctt tggagattta actcattgat ttttatttac
     3421 agttaaaaat caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca
     3481 gctgttgtat ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt
     3541 atttcctaca tattaaaata taatacttcc atttccaata aaaatacatt tataatagga
     3601 aagataaaca gctgaaaagg gagatttcaa atgaagaaaa aaacgttcaa aaaactattg
     3661 tttctagggg gcacagctgc tcttttatta agcggtacaa gttattacct acatatccat
     3721 tctccagaac atacaatgaa cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca
     3781 aaagagaaag cagaagtttt actagatgtt ccattagaga gccagtttga ttctccttct
     3841 ttagaaaatg gctgtgaagt cacttcgctt tctatgcttt tatcttttta tggttatgaa
     3901 acaactaaaa accagttagc tgagcaattg gattacgtcc ctgttttcaa tgcagatggg
     3961 actcatggcg atcctaatga aggatttgtt ggtgatatca gtggcggaga ctgggctatg
     4021 gtcgtctatg ttcccccagt tgcctccctt gctcagcaaa tcgtccaaac ggattatcac
     4081 acttttccaa aaaccgatgc tcaactcgat gatatcaaaa aagcactatc taaaggaaat
     4141 cctgtctgga cttctgtgac tatcaatttt gaagtcccag atgaaactga ttttatgact
     4201 tggaccacaa ataatggaga agtaagagtc actcctcttg ttcatgcttg tgtagtcact
     4261 ggatatgaca aagaaaatat ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta
     4321 ccgatcaacg atttttcggc gattttcact gcaatgggtg gacagatgct aactttggag
     4381 aaaagctagt aattcaaaca aaaatccctt atactcaaga taccaaacct ttgaagggag
     4441 cgtagataat ggaaaaattc acacataaaa aaatggatcc aaatgaaatt ccgattattt
     4501 ttgtccgtga ctgcaaggga aatgttcaag gtaaagtctc tattaatgag tggaacgagc
     4561 gtcgcagacc tgctaccctc aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt
     4621 attacgcaga tcaagagtac gagaaagcca ccgatttatt gaaatttcta attgctcgaa
     4681 cagagtatac tcgctttgaa tatattgaac gtttagcaaa catctatcat attatgaatg
     4741 aacctgtcaa agaatatcag ttattggaca ccgtcttgtc agtagcagaa ctgattgccc
     4801 ttcctgctgg attagaaaag aaactagtac gccgcctttt acgagtaaag caacaactat
     4861 ccgatcaaga aaaataactt gcgttttttt aggaaagtgc tatgattatt ttgttgaata
     4921 aatgggatgt agccaaactg gtaaggcact tgactctgaa tcaagcaatt gtaggttcga
     4981 accctgccat cccaatttga tgtgcttgct gaaaaaatga accccctgta aaagaacaat
     5041 ctttttgcag ggggattatt gtattatttt tactacttgt atttagttta cataattaaa
     5101 ttatgaataa taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct
     5161 aatgctttct ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct
     5221 ttattgctaa ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc
     5281 gttcgttctt tcccattggc atcagcagct ttctgcacat accttgctgt accatattca
     5341 tttacttcct ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt
     5401 agctgatcaa gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca
     5461 aatagcaaaa taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt
     5521 ctacctcctt gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag
     5581 ttggagattt ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc
     5641 gttatttgat attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa
     5701 atcatgttga tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc
     5761 aaggaaatga ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa
     5821 gaacaattag gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac
     5881 agtgtcaaaa acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat
     5941 caaaggagga caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact
     6001 aaaataaacg tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca
     6061 ccacatattt acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc
     6121 tttttacatc aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg
     6181 gattcatttt tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc
     6241 ttttttggta tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg
     6301 gatactataa taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat
     6361 accatttatt ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat
     6421 cacccgaata ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat
     6481 cccactcctt ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca
     6541 gattttaccg gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt
     6601 atcatggact ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta
     6661 tattcctttt tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa
     6721 gttttcagtt ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt
     6781 taggcattgg gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt
     6841 aatttttctt attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat
     6901 gtttaaaaag aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc
     6961 tattctgtta taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct
     7021 ataaattctt gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg
     7081 cttttcgctt cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt
     7141 cctgcttcca tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat
     7201 acgcattatt ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc
     7261 agttttctaa aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt
     7321 ctgttaattt atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata
     7381 attccataaa tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag
     7441 ggaatccttt atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt
     7501 tccactttac taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg
     7561 ctaaaatttc tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt
     7621 cctcccttat ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa
     7681 gaagcggtcg atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg
     7741 atcaaatcaa tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca
     7801 gtttcaacta ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc
     7861 agcatggcta cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca
     7921 ctatgggtca gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc
     7981 tttgctaagt caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct
     8041 accatgatgt tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac
     8101 atctcaagca aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca
     8161 atttctttca agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt
     8221 atttctgcat tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta
     8281 caaaaaacg
//
LOCUS       DACCCM010000103         8245 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCCM010000103 DACCCM010000000
VERSION     DACCCM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.107"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(366..1157)
                     /gene="msrA"
                     /locus_tag="IVK78_002537"
     CDS             complement(366..1157)
                     /gene="msrA"
                     /locus_tag="IVK78_002537"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6375946.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1348..1983)
                     /locus_tag="IVK78_002538"
     CDS             complement(1348..1983)
                     /locus_tag="IVK78_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6375947.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2249..3301)
                     /locus_tag="IVK78_002539"
     CDS             complement(2249..3301)
                     /locus_tag="IVK78_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6375948.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3314..4252)
                     /locus_tag="IVK78_002540"
     CDS             complement(3314..4252)
                     /locus_tag="IVK78_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6375949.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4703..5353)
                     /locus_tag="IVK78_002541"
     CDS             complement(4703..5353)
                     /locus_tag="IVK78_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375950.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5453..5962)
                     /locus_tag="IVK78_002542"
     CDS             complement(5453..5962)
                     /locus_tag="IVK78_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP6375951.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6047..6526)
                     /gene="rlmH"
                     /locus_tag="IVK78_002543"
     CDS             complement(6047..6526)
                     /gene="rlmH"
                     /locus_tag="IVK78_002543"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP6375952.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            6954..8243
                     /locus_tag="IVK78_002544"
     CDS             6954..8243
                     /locus_tag="IVK78_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6375953.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         2615 a         1674 c         1319 g         2637 t
ORIGIN      
        1 atagttattg tttgcgcttt tatacggaat ttagttcatc attaatgtgg aattcgatta
       61 ggtgtctatt aattatcaag atgattctct acaaaattcc atagaaaaat caagattcta
      121 caattcgaat tctctttcac atggaaataa aggctagtaa aaaaacagga gttgtgacaa
      181 aagacttgtc atacctcctg tttccttgcg acttgaagct gaatgctttg cgccacaacc
      241 ttttcctcgg tgttctcaaa gcaactcctc gaaataattc ataactgtcc aaaaatatga
      301 aaagctattt ttgtcagttg ctcattattt tgtcggagct gaacgctttg cgccacaacc
      361 ttcctctatc tatgtcgctg aaaaaataaa tgaagctgat gcgaatgctt gatcttttta
      421 tatcttttag gttgcttctg ataaaattgt tgatgatgat tttcagctgg ccaaaaagtc
      481 tttgcttggc gaatctctgt aacgatagga cggctaaatc ggttagattg aatcagttgt
      541 tctcttgatt tttcagccaa ttgcctttgc ttttcattaa caacaaatag aattgaacga
      601 tattgtttcc cccgatcttg aaattgacca aaggcatctg tagggtctga aatttgccaa
      661 taaatctcca acagctcttc ataggttatt tgacgtgtat caaatatgat ctcaacagct
      721 tcaacatgcc cagtatatcc accactgact tgatcgtaat ttggtttttt ggtctctcca
      781 cctgtataac cagataacac tgagattaca cctggttttg tttcaaaagg ctcaaccata
      841 caccagaaac agccaccggc aaaaatcgcc cgttcttggt ataagggtga agttatctta
      901 tgtttggcat aatcataagc ctttgcactc gctgactccc ctgtgattct taaataaaaa
      961 tccatcgcat ctggtgtcaa attatctcgt agtgctagcg gacgcaagtt gacctctagt
     1021 ttttctatca cctgctttat cgacattttt gctaggtctt cttttgcctc ttcaaattgt
     1081 gttctttccc attctcttgt atttcgattt aaaataatat tataaaggtc tatttccatc
     1141 tgctccacat ctaacatgaa gtcaccctct ttcaagtttt cttcttattc attcaaaaaa
     1201 tgactacctg aaatttagca caacgcataa caagttaaga ctaacttgct tttcgctctc
     1261 ctcatctcta ttcttagtcg aacacatcca cacgaatggt tacccataga gaaattcttt
     1321 tacttaatca gcttcacact gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat
     1381 catcggcaaa tagttaaaga aaaaacttaa gcgttgaaag aagcccaatt gcttaaagaa
     1441 ggcaaactga ctaatcgacg gaatccgtgc taatctgtaa agacctgcaa ttagtaaact
     1501 aataactgtt aaaacaagat aaaaatggct aagcgtacct tgaccacttt gacgataaag
     1561 cagaacaagt aacaatggga aaagtagaaa gcctgcatag cctagcccag aacctatatt
     1621 atggatccaa gtcgatagat tccaagagct ttcattcgta ttgatactga ataacccagt
     1681 aaaaatacaa tcacctatcc catataaacc aactgttgca acagcaagta ttgcaagtgt
     1741 ttttgatgtt ccaataaata ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc
     1801 agatacaact gaccagacca agaaagcacg tctgactgga ctatctacgt caccaaacac
     1861 actaattacg gtagtcattt gattcagttt gggataaaaa aggccaagga tatacggtgt
     1921 cgataaatca ctaatcacac ctaataataa aaaataaaaa ccatattttt gcaaaaaaat
     1981 cattttcttc tcctactttt tagtgattat tcgatacatc caacaatcaa attttttctc
     2041 attttaacac atatttttat tctagttgcc ttttgaacaa tcaataacat aaaggtgagt
     2101 ttcttctaca ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc
     2161 ctactcaaaa aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca
     2221 ttgattgaaa atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat
     2281 catacagttt aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga
     2341 tgactaacca acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg
     2401 gaccaacaag tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata
     2461 gagcgacagg aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt
     2521 ttctaaatag attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac
     2581 ctgacttatt aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca
     2641 tcgcagccgc accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg
     2701 aaataggaga gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg
     2761 gttgtaaggt agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa
     2821 aagggaataa ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca
     2881 caatcgacac tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg
     2941 ctgtcagcat cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac
     3001 ccaaatcagg tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca
     3061 tcattctcat cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg
     3121 ttgctagttg aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa
     3181 aatattttaa aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg
     3241 ctgtaccagc gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca
     3301 ttcaatctct cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca
     3361 agaaatcaca ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat
     3421 tgatataatc gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat
     3481 aatgtaaccc aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga
     3541 catctagttc aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca
     3601 ccattttatg agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc
     3661 atagtccgtt cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt
     3721 cagaaaaatg agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg
     3781 cttcttttcc atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct
     3841 gaccaggacc ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg
     3901 gtacaaattt ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg
     3961 tatctttttt gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg
     4021 taaatagaat aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg
     4081 gtagttttgc ttctacctct ttaccattaa aatttgcttg taatccatgc tctttgattt
     4141 gttggacatg ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca
     4201 ttaacccaaa gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt
     4261 cctccttttt ctttctatta tattcatttg agaatcattt cacaaccttg ttttgttatt
     4321 cttctcttat ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa
     4381 aaactggaat agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag
     4441 aaacattcat agaaaaaacc tcactcctct gattataaaa gagggggcat taacctcgtg
     4501 tatactaaaa ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt
     4561 gtagtacgat gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac
     4621 aatacactta tttaaaaact tctttcccag attttcttcc atttttacag ttataactag
     4681 tattatcatg aaaaatagcc ttttattgtc tatgggaata ataatttaaa attggtactt
     4741 ttttattaat tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa
     4801 ccggaattat ccaagcccag tccaaataat tttttaaaat ctcatcatat tttgcaaata
     4861 tttcattgcc atattccgtc cttaaaatta tattttcagt gccagacaag tgatttgtat
     4921 tgagttgata aaaaagatct ccatctaaaa aaatatcatt agaagaagat ttccccacag
     4981 taatgaccat tccatcagct ctatgtgtta catacaaata attgtatgac ctcatttcac
     5041 ctaatgaatc aattatttct tgccctttag ctctaatata ttgatctcta ttacctatct
     5101 cactcttatt taacaaaagt tttttcatgt caaatttaat cggctgtttt atttttgtaa
     5161 attcgcttct actactaatc caatccgaga ttaatttttc aacatgtccc agagtaaact
     5221 ctgatttatt cgctaaatac tttttttgtt gtattaaaga catctttccc cgaagctcaa
     5281 aattgagcct acatcgattc tctatctctt caaaaagcat cttatcttca tcattcttat
     5341 attcatcatt cataaaacca atccagctcc tcgattaaga taatatttta atctcataca
     5401 aaaactagct ctcatcaaaa caaccattgt tttgatttct catgaaattt ctttatactt
     5461 ttctccgatt ataaattcct aaccccttga gaaaattacg catatattta tctccgcaag
     5521 gcttaaaatt tcgttggcct tcttttctca aaaatgcccc gatttcgcct tttgatggat
     5581 agatttctgc ctcagcaaaa acgtctaaaa tatcatcagt ggtcatagac aaggcgattt
     5641 ttaatttttt tagcaacaca ttgttgatat atttgatttc cttgcttttt tgcggcgtgt
     5701 ctactatcgg cttatctttt tgcggaccac gcgccaagag aatcaggcca tttaggaatg
     5761 cgtcaaaaac ttggttgttg atggtttttt catacacatt attttctgca ttatctttgt
     5821 tcttatcctg tactttggtc agtaccgcct gagcttcttc tttagtcagt ggtacaccgc
     5881 ctagctcaaa agctttaatc aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca
     5941 ataacctatc attgttattc atttgttcct ccgaagtatt tagtctttac tttcttacgt
     6001 actttttgtc ttgctaagat ttttaggatc aatgataaaa accgcatcac ttatgataag
     6061 gcgcccccgc attgatccgg aaggcacggt agatttgctc gacaaggatc agacgcatca
     6121 attgatgtgg atacgtcatt tttccaaaag agatctgagc attgcttctt ttcatgacgg
     6181 cttcgctgag tcctaaagag ccgccgataa caaaaacaag gtggcttttt ccttggatac
     6241 ctaattggtc gatttgttta gcaaaagatt cacttgtagg atttttccct tcaattgcta
     6301 aggcatagac gtattcttgt tctttgattt tcgctaagat ccgttcccct tctttttctt
     6361 ttacttgtct catttgcgct tcgcttaaat tctccggggc tttttcgtca ggaacttcga
     6421 tcaattcgat ttttgcatag gcattcagcc gtttgacata ctcattgata ccttggatca
     6481 ggtatttttc ttttaatttt cctactgaga taattttgat attcatgttt tctctccttt
     6541 ggtcttcatg ctcactttgt ttattataat caattttttc gtctatatgt ttattttggc
     6601 tatttttgtt aaaaagattt ccccaacagt tatgcacaac atccacaggt ttatccacag
     6661 aaaacagtgt tattttacct tatttcatca cattcattag gcaaacatac gttttataag
     6721 aatttatcca ctttatacac aggctgtgga tatgttttat cccttatttt tccaatccta
     6781 tccgcgtagt tttacacagt atctgtggaa aaagaaaatc aaatattttc atctgaaaac
     6841 aatgattttt cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa
     6901 caaataagac tatactaaca gtagataaat gaatgtaatt ggaggaggaa cacatggata
     6961 gaaaaaatgt gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag
     7021 gtctggggct tgttggaggg atcgttggag gactactgac agctggcatt ttttatgcag
     7081 cgatgggcac tggcaacaat agttctactg ctacaagcgg caatcagaat tctgccggtg
     7141 aaacagttgt ggaaaatatc aaagtcaatg tagattctga tatcaccaaa gcggtagata
     7201 aagttcaaga cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa tcaagcggtt
     7261 ttggcggact attcgggcag caagaagaaa gtagcagctc agatgatagt aatttggaag
     7321 cttatagtga aggaagcggc gtgatctaca aaaaagacgg gaatactgct tatgtagtca
     7381 cgaataatca cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa gacggtacga
     7441 aggtaaaagc tgaattagtt ggaacagatg cttattcaga tttagcggtg ttgaagatca
     7501 attcagacaa agtcgaaaca gttgcctctt ttggtgactc aagtgctctg aaggttggtg
     7561 agcctgcgat tgcgattggt tccccactag gatctgaata tgcaaattct gtcacttcag
     7621 gaatcatttc ttccttgaac cgacaagtga caagtacgaa tgaatcgaac gaaacagtca
     7681 atatcaatgc gatccaaaca gatgcagcaa tcaaccctgg aaattctggt ggtcctttag
     7741 ttaatatcga aggacaagtt atcggtatca actcaagtaa gatcgcaagt acttctgaat
     7801 ctacttctaa tgtaagcgta gaaggaatgg gatttgctat tccaagtaac gatgtagtga
     7861 atatcatcaa tcaactggaa aaagacggaa aagtgacacg tccagctcta ggtatcacaa
     7921 tggtcgactt atctgccgtt tctactcagc aacaagaaca aatcttgaag attccttcat
     7981 ctgtaactaa cggtgtcatc gtgacaaatg tccaaacagc tacaccggct gaaaaagctg
     8041 gattgaaaca atacgacgtc attactaaga tcgatgacaa agaagtttct tctggtgtag
     8101 aacttcaatc tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc acttactatg
     8161 aaggctcgaa agaaaaaaca actactgttg aattgacgat cgatcaatcg gcattaaaac
     8221 aaaatcaaaa tagtgaaaat taata
//
LOCUS       DACCCM010000104         8049 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCCM010000104 DACCCM010000000
VERSION     DACCCM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.108"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..596
                     /locus_tag="IVK78_002545"
     CDS             <1..596
                     /locus_tag="IVK78_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP6375954.1"
                     /translation="GDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISDAHKG
                     LVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKN
                     RLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKI
                     IRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            645..947
                     /locus_tag="IVK78_002546"
                     /pseudo
     CDS             645..947
                     /locus_tag="IVK78_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
     gene            complement(1092..1832)
                     /gene="truA"
                     /locus_tag="IVK78_002547"
     CDS             complement(1092..1832)
                     /gene="truA"
                     /locus_tag="IVK78_002547"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6375955.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2023..2658)
                     /locus_tag="IVK78_002548"
     CDS             complement(2023..2658)
                     /locus_tag="IVK78_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6375956.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2805..4046)
                     /gene="sstT"
                     /locus_tag="IVK78_002549"
     CDS             complement(2805..4046)
                     /gene="sstT"
                     /locus_tag="IVK78_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP6375957.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4244..5240
                     /locus_tag="IVK78_002550"
                     /pseudo
     CDS             4244..5240
                     /locus_tag="IVK78_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5256..7274
                     /locus_tag="IVK78_002551"
     CDS             5256..7274
                     /locus_tag="IVK78_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP6375958.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7334..8026)
                     /locus_tag="IVK78_002552"
     CDS             complement(7334..8026)
                     /locus_tag="IVK78_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6375959.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT         2621 a         1558 c         1313 g         2557 t
ORIGIN      
        1 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
       61 caacattttt tgaataccta aaagaacgcg gtttacaagg tacggaactc gttatttctg
      121 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgta agttggcaaa
      181 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      241 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg
      301 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
      361 cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc
      421 gactaaagag taccaatcta attgaacgac tgaatcaaga agtacgcaga agagaaaaga
      481 ttattcgcat cttccccaat caaacatcag ccaatcgctt aattggagcc gttcttatgg
      541 acctacatga tgaatggatt tattcttcaa gaaaatacat caattttgat aagtagaaat
      601 ggtaaaaaca ttgtatagca ttttacacag gagtctggac ttgacttaca attgaattag
      661 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
      721 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
      781 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
      841 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
      901 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
      961 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
     1021 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
     1081 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
     1141 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
     1201 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
     1261 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
     1321 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
     1381 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
     1441 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
     1501 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
     1561 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
     1621 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     1681 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     1741 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     1801 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     1861 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctcctttc ttctctaaaa
     1921 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     1981 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     2041 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     2101 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     2161 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     2221 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     2281 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     2341 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     2401 tagggtagct aacacacgag atgtacttac aaatgcagca tacaatgcag gcaatgacag
     2461 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     2521 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     2581 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     2641 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     2701 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     2761 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     2821 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     2881 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     2941 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     3001 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     3061 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     3121 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     3181 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     3241 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     3301 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     3361 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     3421 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
     3481 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     3541 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     3601 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     3661 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     3721 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     3781 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     3841 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     3901 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     3961 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     4021 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     4081 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     4141 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     4201 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     4261 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     4321 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     4381 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     4441 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     4501 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     4561 caatatcttt tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc
     4621 taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta
     4681 gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac
     4741 tattattttt aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg
     4801 cattacagaa tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt
     4861 tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct
     4921 tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa
     4981 atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt
     5041 tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa
     5101 attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt
     5161 atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa
     5221 atatcgggca gttttttatt tgctatttat tagcagtgtc cctcctagtt atctttttat
     5281 tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga
     5341 ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt
     5401 attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac
     5461 caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag
     5521 cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt
     5581 ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt
     5641 cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc
     5701 tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat
     5761 tgatccttct cctattattg attactagta ttattgtaca acgtagacta caaaaacagt
     5821 attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt
     5881 tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat
     5941 ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca
     6001 ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag
     6061 aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa
     6121 gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc
     6181 ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta
     6241 taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca
     6301 tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac
     6361 cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg
     6421 aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata
     6481 aatttggttt tgacaggttc tattatttag acagtccgga taaattaact tatacggata
     6541 aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt
     6601 tacaaagcaa tacagaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc
     6661 cttatgatga ttactatagt gaattgaact acactgctga aggaactgct gtcattccta
     6721 atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa
     6781 aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg
     6841 accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc
     6901 agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata
     6961 aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag
     7021 taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg
     7081 atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata
     7141 gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc
     7201 gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa
     7261 aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc
     7321 cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt
     7381 cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg
     7441 tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat
     7501 atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc
     7561 cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt
     7621 tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac
     7681 ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg
     7741 atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg
     7801 aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact
     7861 ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc
     7921 tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg
     7981 attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc
     8041 tttttttca
//
LOCUS       DACCCM010000105         7625 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCCM010000105 DACCCM010000000
VERSION     DACCCM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.110"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(14..706)
                     /locus_tag="IVK78_002553"
     CDS             complement(14..706)
                     /locus_tag="IVK78_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6375960.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVK78_002554"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVK78_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVK78_002555"
     CDS             complement(1143..1712)
                     /locus_tag="IVK78_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6375961.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IVK78_002556"
     CDS             complement(1786..2109)
                     /locus_tag="IVK78_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375962.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IVK78_002557"
     CDS             complement(2263..3075)
                     /locus_tag="IVK78_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375963.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IVK78_002558"
     CDS             3429..4277
                     /locus_tag="IVK78_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP6375964.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IVK78_002559"
     CDS             4422..5363
                     /locus_tag="IVK78_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP6375965.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IVK78_002560"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IVK78_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DACCCM010000106         7597 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCCM010000106 DACCCM010000000
VERSION     DACCCM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.111"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(135..425)
                     /locus_tag="IVK78_002561"
     CDS             complement(135..425)
                     /locus_tag="IVK78_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375966.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IVK78_002562"
     CDS             complement(543..803)
                     /locus_tag="IVK78_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375967.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IVK78_002563"
     CDS             complement(940..1353)
                     /locus_tag="IVK78_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP6375968.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IVK78_002564"
     CDS             complement(1809..2294)
                     /locus_tag="IVK78_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375969.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="IVK78_002565"
     CDS             complement(2433..2678)
                     /locus_tag="IVK78_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP6375970.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="IVK78_002566"
     CDS             2871..3671
                     /locus_tag="IVK78_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6375971.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="IVK78_002567"
     CDS             3690..5558
                     /locus_tag="IVK78_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6375972.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNAVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="IVK78_002568"
     CDS             5551..6042
                     /locus_tag="IVK78_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP6375973.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="IVK78_002569"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="IVK78_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP6375974.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..7597
                     /locus_tag="IVK78_002570"
     CDS             6602..7597
                     /locus_tag="IVK78_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP6375975.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1215 c         1442 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atgcagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctga
//
LOCUS       DACCCM010000107         6962 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCCM010000107 DACCCM010000000
VERSION     DACCCM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.112"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..193
                     /locus_tag="IVK78_002571"
     CDS             <1..193
                     /locus_tag="IVK78_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375976.1"
                     /translation="GRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPL
                     VPKGYDFLDSISKLEKMND"
     gene            271..945
                     /locus_tag="IVK78_002572"
     CDS             271..945
                     /locus_tag="IVK78_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6375977.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1178..1612
                     /gene="dcm"
                     /locus_tag="IVK78_002573"
     CDS             1178..1612
                     /gene="dcm"
                     /locus_tag="IVK78_002573"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6375978.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1613..2320
                     /locus_tag="IVK78_002574"
     CDS             1613..2320
                     /locus_tag="IVK78_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6375979.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2310..2600
                     /locus_tag="IVK78_002575"
     CDS             2310..2600
                     /locus_tag="IVK78_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375980.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2859..4043
                     /locus_tag="IVK78_002576"
     CDS             2859..4043
                     /locus_tag="IVK78_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6375981.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4058..4177
                     /locus_tag="IVK78_002577"
     CDS             4058..4177
                     /locus_tag="IVK78_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP6375982.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            4923..6833
                     /gene="ltrA"
                     /locus_tag="IVK78_002578"
     CDS             4923..6833
                     /gene="ltrA"
                     /locus_tag="IVK78_002578"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6375983.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         2437 a         1118 c         1539 g         1868 t
ORIGIN      
        1 gggtcgtatg agtgaactcg gatattccat gatgtttggg gaggtcgaaa aaaacttctt
       61 tatgaagcac atcaaaggtc gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt
      121 ttatacgcca cttgtaccaa aagggtatga tttcttagat tcaattagta aattagaaaa
      181 gatgaatgat tgagtgatct tcaatactga ctttatactt caagtaacgt ataatacttt
      241 tatatagtag gaagaaaggg gataattaat atggaaagta atgattctta tttagagtat
      301 gcaaaagcaa ataaggataa gtttatttct tatattatca aagggaaatc ccccgaagtt
      361 ggacgagacg cagtttttat ggcagggagt cctggggctg gaaaatcaga ggttgccatt
      421 ggattagctg agaattatga taaccatgtg attattgatg cggattactt tcgcacccag
      481 tttcctgatt ataatggaaa aaactctagt ctctttcaaa aagcatcttc ttggttggta
      541 gagcaggcat ttagatatgt cttagatcat ggctattctt ttattctgga tgcaactttt
      601 gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga aaaaacattt tcgagtgacg
      661 attttctatg tttatcaaga ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt
      721 gcagagggac gtcatgtgcc gaaaaaggca tttattaacg cttttttcaa atcaagagag
      781 aacattgcaa aagtaaagag taaacatcca gatgtattgc ttcatatcat aataaaagat
      841 tatacgaata atatttccga agttcattat gctacggata atctagaatt atttttacca
      901 ctagggtata gtaggaaaga tttggaggtt caattacatg actgagaaag aacaagtaac
      961 gaaaattgta aagaaataca ataagagcat tgcggattta tcggaaaatg ctactgcaaa
     1021 agagttcaaa acggtgatta aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt
     1081 agttggatta aataaaaaat agtaggacaa attaataatg tatgagccaa gagcaaaatg
     1141 ttcttggctt ttttctttgc aatgaatgag gtaatgtatg agatttttag atttattcgc
     1201 cggtattggt ggctttcgtt taggtatgga acaggcgagc catcattgta tcggtttttg
     1261 tgaaattgat aaatttgcta gacgtagtta taaagcaatt catgatacaa gtaaggaggt
     1321 ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt caatccctcg gaccagtgga
     1381 catcctttgc ggcggatttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt
     1441 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcaccactc ttctccgacc
     1501 taagctttta ttccttgaga acgtccgagg attgctcaat cacgaaggag gggctacgtt
     1561 cgagacgatc ctccgaacgc tggatggatt ggggtatgac gtggagtggt aggtgcttaa
     1621 ctcaaaggcc tacattcccc aaagccggga gcggatattc cttatcggac attctcgaga
     1681 cgcatgtacc gaacaagtat ttcctatcat tggaccgtct tcaacatctg atcaaaacat
     1741 cagaaatttg gtaaatatca atccttccaa tcgaggaatg ggtggacaag tctatggttc
     1801 agatggcgta gcgccaacct taacaggtga tgaaggaatc aaaatcgcac tgccagcaag
     1861 ggatggaatc tctgtagcag gactattacc gggaaagttt gaacaaggta atcgagttta
     1921 tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat
     1981 tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa gacagtgtga atctggcttt
     2041 tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag agtgttcata ccttactaac
     2101 aggagaccaa caagcgatcg tcacggatca gtatcaaatt cggaagctaa cacctagaga
     2161 gtgttggcga cttcagggct ttcccgattg ggcttttgat cgagattctc aagtcaattc
     2221 agatagtcag ttgtataagc aagccggaaa ctctgtaaca gttccggtca tttttgatat
     2281 tgccagaaga ttaaaagagt tggatgtaca tggtttatga tgagttgatt ggagaaattt
     2341 attgggtgat tggaaagatt cagtcagacc cagaattaga agaacaattg cgacggttaa
     2401 aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga tccatattta atgaatgagg
     2461 agacggatgc tcgaattgaa ataaatcaag taattgcaga atttgagcgg attgcggacc
     2521 aagcaaaaga accagatatt cgtcaatact tgttagaaat gaaagcagag ttggaagtaa
     2581 atggtattac agatgagtaa cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga
     2641 ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga
     2701 ccatgatgtc tcacccccgt atctaacagg ggggtacaaa aacacaaaaa ataagtaagc
     2761 aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa tttgtatagg tgttaactca
     2821 tttgaaaaag ttaacacctt gaatttggag gtgagaactt ggcacaacgg aatttagatt
     2881 atcggctatt aaaagaccga cgcaacgaat acggcgtttc acaaaataaa ttggctacgg
     2941 cttgtggact tagtcggccg tacttaaatc agatagaaaa tggtggcgta accgcatcta
     3001 taaaaaccat gaggaaaatt tttgaccaac tggaaagctt taatcccgat ttgcctttga
     3061 cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcacga aaaattattc
     3121 aagaaattct ccatttgaaa tttgagtata tgctccatga agattatgcc ttttactcct
     3181 atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt gtctcatgaa gaagataaag
     3241 gtgtgctttt agaattaaag gggcgaggtt gccgacaatt tgaaactttt ttactcgctc
     3301 aaaagcgtag ctggtacgac tttttcgagg attgtctaaa ggccggtggc gtgatgaaac
     3361 gcttggactt ggcaatcaat gatcgagtgg ggctattgga tatacctgag ctaacgaaaa
     3421 aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt taaaagttat cgttccgggg
     3481 aacttttaaa ggctgatgaa aaggacggca tgggcaatac cttatatatc ggcagtctca
     3541 agagtgaggt ttatttctgt ttgtatgaaa aagactatga acaatatatc aagttaggga
     3601 ttccattaga ccaaactgag acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg
     3661 cttttcatgc gattcaagat ttgttaaaag gtcgtagcat tgagagcacc acgttttcca
     3721 ttatcaatcg ttacttgcga tttgcggata aagtagaagg taaaaggcga accaactggc
     3781 ccttgaatga acaatggggg cgttttattg gacggaatcg gaaagagatt caattaactt
     3841 ccgagccaaa accgtatacc attgaacgaa ccttgaattg gctaggccga caagtcgccc
     3901 ctacgtggaa aatggctaag gagttggatc gattgaatcg gacaacctat atccaggata
     3961 tggtacagaa tgcacgctta tcagaccgac ataaaaagat tttggagcaa caaagtatgg
     4021 caattgaaaa tttgattata tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt
     4081 tttggtattt ggtggcagta tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc
     4141 agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt
     4201 tatgtgcgac tcgttcctta gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg
     4261 gttattagga gaactgataa tctgacaggt ggaatgatga agtaacgctc tgaaacgcct
     4321 gccctgagcc tccgacaagc attgtatgag gaaactcaga atgtttgaag ctcggtaaag
     4381 acggctgaaa gataccgtaa tagttgatga acatatatct tctatcgaaa actatccaga
     4441 ggtgggtagc gtatccaaca ggtcgggggt ctataaaata tctatggtta gaatgttcta
     4501 taaggaactg acgaaacagc gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa
     4561 ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat aggttatgaa atgccatata
     4621 acgttacagg cggtatcaag ccagcaggct tatagctagt gacctaagga tatatatgta
     4681 tagataagat tatcggaacg aggaaaggta ttgggttctc atcaagagaa taaactgacg
     4741 aagaacaata agcagttgaa ctaatgctga aaagcagagg gcgaacctaa gaaattcttg
     4801 taatgagaat ggaggaatgc cctcaagtcg tgtatatatt agaaaagtat tatgttgttt
     4861 caataaggat tacgagtaag ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc
     4921 ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga tttaagacac gctgaatatt
     4981 acgaaatgca aaacaccttt gatgatttat atgccaaaag taagaatgga gatattttca
     5041 ctcatcttat ggatattatc ctatcgagag aaaatatatt actggcatat cgaaatatca
     5101 aagcaaatgc aggcagtaaa acagcaggaa cagacggtac aataattaaa gatattggca
     5161 aattgccagc agaaacagtt gtcaaaaaag taagatacat tgttgcaggc agtccacatg
     5221 ggtatcgacc taagccagtg aggcgaaaag aaataccaaa accaaatggt aaaaccagac
     5281 ctctaggtat tccttgtatg tgggacaggc ttatacaaca atgtataaaa caagtattag
     5341 aaccaatctg cgaagctaaa tttagtgaaa acagctatgg ttttagacct aataggtcag
     5401 ttgaaaatgc aataaaagca acctataacc gcctacaaat cagccagcta cattatgtga
     5461 ttgaatttga tattaagggt ttctttgaca acgtgaacca ttccaagctg ataaaacaga
     5521 tatgggctat gggaatacgg gacaagcact tgatattcat tttaaaaagg atattaaaag
     5581 ctccaatcaa aatgactaac ggtacaataa catatccaga gaaaggaact ccgcaagggg
     5641 gaataatatc cccactacta gcaaacattg ttcttaatga gttagaccat tgggtagaaa
     5701 gccaatggca ggaaaatcct gttacaaaaa actatgtggt tcatatcaat aaaagcggta
     5761 gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata
     5821 tggttcgtta tgcagatgat tttagagttt tctgccgcta caaggaaagt gctgaaaaag
     5881 ccaaaatagc aatcactcaa tggattgaac aacgattaaa attggaagta tcacaagaaa
     5941 aaacaagaat tgtaaatgtt agaaaaagat actccgactt tcttggtttc aagattaaaa
     6001 tgataccgag aagaaagaag ttagttgtta aatcacatat aagtgataaa caattcaaaa
     6061 accaaaaaga caagctggta tcacaggcaa agaaaattgc ttctccctca aaagggaaaa
     6121 cggaattaga agaaacaaga ctttataatt caatggtatt aggtatgcaa aattattatc
     6181 agattgcaac gaatgtaaat ctggattgta tgaaactgaa tagagctgta atggctatct
     6241 tcactaacag attaggtaca aacaaaaggg gcagattgag aaaaacaggc agacaactta
     6301 cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg
     6361 gcgaacctat tttccctgtt ggatatatcc aacacagaaa accaatggca aaggtgtatt
     6421 tagcgaattg ctacactcta gagggaagaa aatttatcca caccaatcta agtgttgata
     6481 aattactgat gtaccagtta atgaaattat ctttggaaaa tcgcacaata gaatacgcag
     6541 acaatcgaat ttcgctattt tctgctcaac atggaaaatg tgctataact ctggaagaat
     6601 ttcaacaagc taatgaaata cattgtcatc atattgtacc aactggagct ggtggaaatg
     6661 acgattataa aaatcttatt ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa
     6721 cagaagaaac aatacaaaaa tatatagctc tattgaagct aaataaagtc cagctcataa
     6781 gactaaataa atatagagta ttagctggca atcaagaatt aaacttgata taggtctact
     6841 acataaatac ttttcaatat aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct
     6901 ccgtgtgaat ggggggaaaa gtttgagata tttataatat caggaactta cctatccata
     6961 ta
//
LOCUS       DACCCM010000108         6851 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCCM010000108 DACCCM010000000
VERSION     DACCCM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.113"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1..859)
                     /locus_tag="IVK78_002579"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IVK78_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IVK78_002580"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IVK78_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6375984.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IVK78_002581"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IVK78_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IVK78_002582"
     CDS             complement(2143..2373)
                     /locus_tag="IVK78_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP6375985.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IVK78_002583"
     CDS             complement(2603..3421)
                     /locus_tag="IVK78_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6375986.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IVK78_002584"
     CDS             3582..4271
                     /locus_tag="IVK78_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6375987.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IVK78_002585"
     CDS             4285..5787
                     /locus_tag="IVK78_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6375988.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IVK78_002586"
     CDS             5800..6276
                     /locus_tag="IVK78_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6375989.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IVK78_002587"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IVK78_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2223 a         1080 c         1250 g         2298 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgtatggaa tggcaacagt
     6661 ttttttgaca aattttataa ggtgcagaac ttctttccgt atgctattcc gattgtcctt
     6721 gacaatgagc ctcctcggat gtgacgatct tcggcaggag ctaacagtta aagttagact
     6781 gcctcgctag cgttagcaca tccgagctca ttatcaagga ttcgctgcgc caatctctgg
     6841 ttacggtaac c
//
LOCUS       DACCCM010000109         6801 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCCM010000109 DACCCM010000000
VERSION     DACCCM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.114"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..464)
                     /locus_tag="IVK78_002588"
     CDS             complement(<1..464)
                     /locus_tag="IVK78_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6375990.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALP"
     gene            complement(1691..2380)
                     /locus_tag="IVK78_002589"
     CDS             complement(1691..2380)
                     /locus_tag="IVK78_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6375991.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(2425..3189)
                     /locus_tag="IVK78_002590"
     CDS             complement(2425..3189)
                     /locus_tag="IVK78_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP6375992.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(3217..3915)
                     /locus_tag="IVK78_002591"
     CDS             complement(3217..3915)
                     /locus_tag="IVK78_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP6375993.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3927..4706)
                     /locus_tag="IVK78_002592"
     CDS             complement(3927..4706)
                     /locus_tag="IVK78_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP6375994.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4722..5666)
                     /locus_tag="IVK78_002593"
                     /pseudo
     CDS             complement(4722..5666)
                     /locus_tag="IVK78_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5712..6491)
                     /locus_tag="IVK78_002594"
     CDS             complement(5712..6491)
                     /locus_tag="IVK78_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375995.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1890 a         1430 c         1094 g         2387 t
ORIGIN      
        1 ggtaaagcag gtctgggtcc aataatactc atatcacctt taagaatgtt ccagagctga
       61 ggtaactcat cgatacttgt ctttcgaata aatttaccaa ctctcgtgat tcttgggtca
      121 tctttaatct tgaagaccgg accatccatc tcgttttgtg ccaacaaggc ttctaatttt
      181 gcttcagcat ctacacacat agatcggaat ttataaaact tgaacagctt tccatttctt
      241 ccaactctat cctgcacaaa aatcgggctg ccaccgggat cgtcaatcac aatggcgagc
      301 gctgtaatta gcatcacagg gctcaatgca atcaaagcta gagccgagaa gaagatatct
      361 tgtgctctgc gtccaatcca atagaatcta tgtcccctaa gattctcttc cctatcaatt
      421 gttacgtctt tctgaacacc tttcttttct tctataccaa tcatcttttt ccattcgtcc
      481 ctctattttt acaagtctca tcatctgtaa acgacttaaa ggcctgtgat catcgaccac
      541 tctatctgaa acagttctca tcgaaccatg ttcattccta ctaaaagcag ccaactccaa
      601 cgccggcaca ttcaattttc atatctatct cctcctcgtt taggggagaa atacgagttt
      661 aggtcatttt tattgacctc gtttggggct atattagaat ttcacattga aatgtactta
      721 cattctcaac accatcattt tccaaaaagt attatgtagg tacatattga cctctaatcc
      781 caccccaaag gcaggtttga aattcaatga ttgagctatt ctcaccccaa agatgagttt
      841 atcgaacaat ttattgggct attactaccc caaactgtct atttacagca aatttatgat
      901 tttatacatt gaaattttcc aatccgaaaa acccaaacta attttctgcg ttgtagacat
      961 tggtaaatac ttgaacaact tcacggctat catcatcaca tcctacgata ataattctaa
     1021 ttggataaag attatcatca ataatttact tccacgtaaa aattctgaac taaataaaat
     1081 ctattgataa tattttttct tccatttttt gtatacccta caggtatagt gaagttaatt
     1141 atccccaatg tctctgaagt aacagacatc actaattcaa taacacattc actaccatag
     1201 tttgtaggag cagaaatgac aacaaactca gcctccttat gtgctgtttt tccatctatc
     1261 caaattttat tacataggat tcactaaaat tttaccgttt attttttcga ttcttttttt
     1321 gaaccgtccc atgaacatac cgattcaaca agctaaatta taaaaattgg tgtgctgtca
     1381 ttattttttg gaaaatttat taatggctat caatatactt ttgtatcata ctaaatagct
     1441 cgtcagttcc tatatcggat ataccaatat taaagcattt agatcattat atttaagagt
     1501 ggtatcatcc caaaatttcg aatattttct tttatataag aataatgata ctgaattgcc
     1561 ccattcacct ttcttttttt agattttcta caagtgattc aaaccctcca taattacagt
     1621 tattactttt gaaacgataa tatgaacatg tgtaatcttt tttattaaca atccatcatt
     1681 ctatccctaa ctaatatgct ccattgggct tgacaataac ataaaacgtt tttaaaatca
     1741 acctcaagtc gtacattaaa cttcgatgac taatatactc taaatccaac tcaaccattt
     1801 tatggaaact aacattattt ctatcactaa cttgccacaa acctgaacaa ccaggtttta
     1861 catacaagcg ttgaagatca tatctcgtgt actcagctac ttccctctcc aaaggtggcc
     1921 gtggaccaat tagactcata tcgcctttta aaacattcca tagctgtggc aattcatcaa
     1981 tgcttttggc acggataatt ttacctattt tagtgacacg gggatcttct ttcatcttga
     2041 acattgcacc ttctatttca ttttgatcaa gtaattcttc caatttttct tcagcatcta
     2101 aacacatcga acggaattta tacattttga atgtttttcc atttttacca acacgtgtat
     2161 ggcaaaaaaa tatcggactt cctggctctt cttttctgat tcgataagct acatagaaca
     2221 tcaaaggaga agctaaaatc aaaccaataa tgcttcctac aatatcaatc attcttttaa
     2281 aaaataaata gatatatcgt ttttcaactt tttctttatc caaccaaact tgattaggat
     2341 gtacttcact aatacgagat aataaatctt cactttccat actttccctc tctcctcatt
     2401 tcctttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     2461 atagactgga taactgattt catccccgtt gatcagatcc tttgcgactt gtttcatctg
     2521 tactactttt tccttaccaa agtccttagc aatctgctca tagcactctt ttaagtaaaa
     2581 ggtacgctta gagaccccat gggcatcaga agcagccatc tgcacgagat tatgtttgac
     2641 cattgttttg gctgttttct gaatgtcttt gccaaataca ccaacataac ttggcgcagt
     2701 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2761 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2821 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2881 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2941 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     3001 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     3061 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     3121 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     3181 atcgatcatt tctcttgtct cctctcttct tattccttaa tttccgtagt aataaccagc
     3241 atctttgcta tgttctgctc cgttgtatac aacacctaaa actcgtgctt gtaccatctc
     3301 tagcagttct tttgctttat tcaatgattc tttacgtgat gtattttcac gtacgaccaa
     3361 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     3421 catatcgaat atcaccacgt caaataactg tcttgcttcg tctaggatct gatccattct
     3481 tggcgaactt aacaattctg atgggttggg tggcttaggt ccactaggca aaatcgacaa
     3541 gttctcgact ggcgttcgtt ggattacatc tgctacactt cctgatgaac taagtgctgt
     3601 acttaatccc ctggcattgt tcagctggaa tgtcttatag acgacgggtt tacgtaaatc
     3661 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3721 agtggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3781 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3841 tgaagatggg tttgttaatg tgatcaaact gactgcatga gtttgtgctg ctttttgttt
     3901 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggggct
     3961 gttttatcgt ttgtagcttt tggtcgtgca ggagctgttc cttttttacg gataccagcg
     4021 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgcatcc
     4081 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccagacct
     4141 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     4201 acaggtgtcg tattggcaac tgcacctgaa ataatcgaga ttttatcgac attcatcacg
     4261 tcttttgcgt tctcttggaa aacttgtgaa gttgtgttag cgatctgttg agcagtcact
     4321 gcattcgtgc ttattgcctg gatcgagaac atttgtgaat tttgtgactg attgacggag
     4381 atcgcatctt tgatttgtcc agcagtcatt ttcacattgt cttctgtttc taaacgatct
     4441 ttgacttcat ttagtactaa atcactgacg atcatatctt tatacgtatt gatcatttgc
     4501 aggttcgtat tgacatcatt gacatttgct gtgtctgatt gtggcagtgt cacgatcaat
     4561 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     4621 aagccagcaa acattgagat gatgattgtt accaagtgtt tgcgtaatgt ttcaaaaatt
     4681 tcttccaagt tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4741 atttgatctt ttaattcttt ttttacacga tcaagttctt gatcactgac tttttgatag
     4801 gagatattat cttgcataaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     4861 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     4921 ttcgactcta gagcattcaa aacggattga tagttgctga caccgtttaa actcaatact
     4981 ttttttgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     5041 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     5101 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     5161 ccaactgcat caacaagttc ctttagccct ttcatattga tagagacata gtgatcgatt
     5221 gggatgtcta catattattg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     5281 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atcacgagga
     5341 atgctgacga tcgttgtctg tttgtcttca ggactgacta tggcaaccat catcgtatct
     5401 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     5461 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     5521 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     5581 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     5641 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcaa
     5701 cgttttaact gtcaataact tatattgcta tagcccattt gctgagcaac agtttgtgct
     5761 tttgttgcgt atgctgcgct tattttttca ttccggatct gctctatttg actgagcgcc
     5821 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catccttcaa catcgtatcg
     5881 aataactgat ctagttgcgt gacttgcgcg atctgagtat cagcgaatcc taaatattgc
     5941 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     6001 acatctgcac ttgtcacctt ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     6061 gatacatttt tagtaaacaa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     6121 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     6181 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacatagga tagcttattt
     6241 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     6301 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     6361 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtaacca
     6421 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     6481 ggcttttcca tagattcact cctaagtatt gatgatagat aaattaagat ataattcact
     6541 tacataaaaa tgacagcttt ttattttaaa aaacataatt aaactaatat attcacagaa
     6601 atatttgtaa acaagttttc cctatataat cataccacaa actgttttct tgactagggc
     6661 aatttatgat ttaaaagtaa ataaaacatt aataagataa caattaatcg atttcatttt
     6721 tatttcctta attttcatag gtgtttttaa aagttacaca aaaaataaaa taacaaaaaa
     6781 attaacattg aattaataat a
//
LOCUS       DACCCM010000110         6462 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCCM010000110 DACCCM010000000
VERSION     DACCCM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.115"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(109..564)
                     /locus_tag="IVK78_002595"
     CDS             complement(109..564)
                     /locus_tag="IVK78_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6375996.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IVK78_002596"
     CDS             complement(718..1926)
                     /locus_tag="IVK78_002596"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP6375997.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IVK78_002597"
     CDS             complement(2038..2370)
                     /locus_tag="IVK78_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6375998.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IVK78_002598"
     CDS             2518..3393
                     /locus_tag="IVK78_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6375999.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IVK78_002599"
     CDS             complement(3475..4056)
                     /locus_tag="IVK78_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP6376000.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IVK78_002600"
     CDS             complement(4062..5321)
                     /locus_tag="IVK78_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6376001.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IVK78_002601"
     CDS             complement(5323..6369)
                     /locus_tag="IVK78_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376002.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1799 a         1338 c         1102 g         2223 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcactt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aa
//
LOCUS       DACCCM010000111         6287 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCCM010000111 DACCCM010000000
VERSION     DACCCM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.116"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..177
                     /locus_tag="IVK78_002602"
     CDS             <1..177
                     /locus_tag="IVK78_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376003.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..691
                     /locus_tag="IVK78_002603"
                     /pseudo
     CDS             190..691
                     /locus_tag="IVK78_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            788..2635
                     /locus_tag="IVK78_002604"
     CDS             788..2635
                     /locus_tag="IVK78_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP6376004.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2638..3534
                     /locus_tag="IVK78_002605"
     CDS             2638..3534
                     /locus_tag="IVK78_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6376005.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3584..3973
                     /locus_tag="IVK78_002606"
     CDS             3584..3973
                     /locus_tag="IVK78_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6376006.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3960..>6287
                     /locus_tag="IVK78_002607"
     CDS             3960..>6287
                     /locus_tag="IVK78_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6376007.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1349 g         1887 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
      301 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
      361 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
      421 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
      481 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
      541 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
      601 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
      661 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
      721 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
      781 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
      841 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
      901 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
      961 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     1021 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     1081 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     1141 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     1201 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     1261 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     1321 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     1381 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     1441 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     1501 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     1561 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     1621 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     1681 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     1741 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     1801 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     1861 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     1921 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     1981 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     2041 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     2101 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     2161 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     2221 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     2281 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     2341 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     2401 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     2461 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     2521 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     2581 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     2641 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     2701 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     2761 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     2821 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     2881 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     2941 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     3001 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     3061 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     3121 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     3181 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     3241 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     3301 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     3361 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     3421 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     3481 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     3541 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     3601 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     3661 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     3721 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     3781 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     3841 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     3901 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     3961 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     4021 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     4081 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     4141 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     4201 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagaggca ttagtatcca
     4261 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     4321 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     4381 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     4441 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     4501 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     4561 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     4621 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     4681 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     4741 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     4801 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     4861 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     4921 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     4981 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     5041 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     5101 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     5161 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     5221 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     5281 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     5341 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     5401 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     5461 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     5521 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     5581 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     5641 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     5701 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     5761 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     5821 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     5881 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     5941 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     6001 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     6061 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     6121 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     6181 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     6241 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaac
//
LOCUS       DACCCM010000112         6202 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCCM010000112 DACCCM010000000
VERSION     DACCCM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.117"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            191..709
                     /locus_tag="IVK78_002608"
     CDS             191..709
                     /locus_tag="IVK78_002608"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6376008.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            837..1520
                     /locus_tag="IVK78_002609"
     CDS             837..1520
                     /locus_tag="IVK78_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP6376009.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1829..1902
                     /locus_tag="IVK78_002610"
     tRNA            1829..1902
                     /locus_tag="IVK78_002610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Arg,seq:tct)
     gene            2061..2420
                     /locus_tag="IVK78_002611"
     CDS             2061..2420
                     /locus_tag="IVK78_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP6376010.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2543..4174
                     /locus_tag="IVK78_002612"
     CDS             2543..4174
                     /locus_tag="IVK78_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP6376011.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4236..4877)
                     /locus_tag="IVK78_002613"
     CDS             complement(4236..4877)
                     /locus_tag="IVK78_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6376012.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5159..5467
                     /gene="rplU"
                     /locus_tag="IVK78_002614"
     CDS             5159..5467
                     /gene="rplU"
                     /locus_tag="IVK78_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP6376013.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5494..5838
                     /locus_tag="IVK78_002615"
     CDS             5494..5838
                     /locus_tag="IVK78_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP6376014.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5851..6144
                     /gene="rpmA"
                     /locus_tag="IVK78_002616"
     CDS             5851..6144
                     /gene="rpmA"
                     /locus_tag="IVK78_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP6376015.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1951 a         1023 c         1313 g         1915 t
ORIGIN      
        1 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
       61 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
      121 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
      181 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
      241 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      301 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      361 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      421 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      481 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      541 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      601 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      661 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      721 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      781 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      841 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      901 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      961 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
     1021 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
     1081 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
     1141 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1201 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1261 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1321 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1381 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1441 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1501 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1561 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1621 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1681 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1741 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1801 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1861 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1921 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1981 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     2041 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     2101 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     2161 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2221 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2281 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2341 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2401 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2461 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2521 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2581 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2641 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2701 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2761 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2821 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2881 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2941 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     3001 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     3061 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     3121 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3181 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3241 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3301 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3361 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3421 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3481 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3541 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3601 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3661 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3721 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3781 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3841 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3901 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3961 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     4021 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     4081 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     4141 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4201 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4261 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4321 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4381 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4441 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg
     4501 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4561 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4621 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4681 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4741 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4801 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4861 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4921 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4981 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     5041 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     5101 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     5161 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5221 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5281 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5341 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5401 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5461 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5521 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5581 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
     5641 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5701 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5761 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5821 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5881 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5941 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     6001 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     6061 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     6121 tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt
     6181 ttgtcacagc cttttttgtt ta
//
LOCUS       DACCCM010000113         6168 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCCM010000113 DACCCM010000000
VERSION     DACCCM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.118"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            725..811
                     /locus_tag="IVK78_002617"
     CDS             725..811
                     /locus_tag="IVK78_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6376016.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVK78_002618"
     CDS             complement(932..1621)
                     /locus_tag="IVK78_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376017.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVK78_002619"
     CDS             complement(1628..1906)
                     /locus_tag="IVK78_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376018.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVK78_002620"
     CDS             complement(1866..2780)
                     /locus_tag="IVK78_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6376019.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVK78_002621"
     CDS             complement(2762..3142)
                     /locus_tag="IVK78_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6376020.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVK78_002622"
     CDS             complement(3592..3879)
                     /locus_tag="IVK78_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6376021.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVK78_002623"
     CDS             complement(3899..4123)
                     /locus_tag="IVK78_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP6376022.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4758..5291)
                     /locus_tag="IVK78_002624"
     CDS             complement(4758..5291)
                     /locus_tag="IVK78_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6376023.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5312..6154)
                     /locus_tag="IVK78_002625"
     CDS             complement(5312..6154)
                     /locus_tag="IVK78_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP6376024.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1157 c         1044 g         2333 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtatactatg ccaagtagcc
     4321 cctctaccaa tggatagaga cgctcacttt gtgattttta attggtctag gtcaaaagct
     4381 ctttttaagg cttgattttc tgcagttgct tctttccgtt tctttgtgaa tttttaccag
     4441 tcatttttta ctggggtgtt atttttcata aaatatgata aatttttggt agcgcttcat
     4501 aagcgcgttg ctttttcaag aataaacact cattattttt taactggctg ctcggaagag
     4561 cagtcttttt tattatttat ccgtttcttt tacaaacaat tcttcgttag gactgttgta
     4621 gtaattagga ttgagtttta gttccccttt tttatttcct ttgttggcca ctgataataa
     4681 cattgggcta aagcttaata ttcctagtag gatagaaccg aatagcaagt gtttttttgt
     4741 cataagtatt tccttcttca tttccatagc tgccaccatt ttttccaatt ctttgtttgt
     4801 tcgtccattg tttctgttgc ttcagaaaga gtcgttgaac tattctctga agactcttcc
     4861 gtataagtca actgcagttg ctcctgaagt ttttcgattt gttggttggc ttgcagggtg
     4921 agttgttgtt gctggtcgag tagtttttgt atatgagaat tattttgctc tttgagggca
     4981 agttgttctc gtaaaaattc attttcttca cgcaactgat ctagcaactt gtttgcaacc
     5041 acgtttttgt ttggtatata agcattttta tcgttgtttt gttcatgttt atgcaactta
     5101 tctagcaacc tttgattgtc taagttgcga gcaagttgca atactccaga acgcaataca
     5161 accttataac cacgctcatt tttagctatc atggaagaat ctaatttttg gatatgtttc
     5221 gatattgctt gcctactcat cttaaaaaga tctgacaatt cctttattgt aaaggtttct
     5281 tgtggttgca tactggttgc ctcctaggtt gctactggaa taaattatct aaatcagata
     5341 ataaactttt tttctctttt tccgaaagtg cggactctgt ttgtttttca agagcttttt
     5401 tctctttttc tagttgtttg atttgttgga ggagttcaag ttctcgtttc ttctgtttga
     5461 ttttttcgtt aattgttgta gcgggttctt cgtattcttt ttgtttatca gctaaagcca
     5521 atgctttatt ttggtcttct ttggttggtg catgtttgtt aaagtcgtct gcttttttga
     5581 tctcaggttt gaacgtaaat tgatagccaa taacaggttt tccccgtcct tttccgtatt
     5641 tctttttgat tgttagtcct ttgaagattg cagttagttc ttgacgtata ggttttataa
     5701 ctcgtttatc tatatcaggt tgcttataac ttttaggaat atctaataat tcaagaaagt
     5761 cttctttgga gaaatatgcg aaacctttgg ttctgtattg ctttaacaga cgaaacatag
     5821 tctttgcata gccactttgt agctcgttga attgttgtaa agaatagcgt acccactcgt
     5881 ctaaattatt taacaaaggc agtgcttttt cgtaaattcg tatatctacg taaggatttt
     5941 ctacgtcccc gttgatttta aattctgtaa acattacaaa catatcgtaa gaaagcccag
     6001 actcacttct acgaccaaag cgtaaagata agattttttc ataagttgct tttaaatctt
     6061 ctacaaatcg tacatttgca gttgctttgt agttacttaa atcttttagt tggtcaaacg
     6121 taaaacgaac tgtcttatcc cctttttcac gcattctaga cacgatgg
//
LOCUS       DACCCM010000114         6068 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCCM010000114 DACCCM010000000
VERSION     DACCCM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.119"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            832..1566
                     /locus_tag="IVK78_002626"
     CDS             832..1566
                     /locus_tag="IVK78_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6376025.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IVK78_002627"
     CDS             1582..2751
                     /locus_tag="IVK78_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6376026.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IVK78_002628"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IVK78_002628"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6376027.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IVK78_002629"
     CDS             3841..4767
                     /locus_tag="IVK78_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP6376028.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IVK78_002630"
     CDS             4786..6036
                     /locus_tag="IVK78_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6376029.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1247 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DACCCM010000115         5972 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCCM010000115 DACCCM010000000
VERSION     DACCCM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.120"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(313..2205)
                     /locus_tag="IVK78_002631"
     CDS             complement(313..2205)
                     /locus_tag="IVK78_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6376030.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IVK78_002632"
     CDS             complement(2202..3929)
                     /locus_tag="IVK78_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6376031.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IVK78_002633"
     CDS             complement(3945..4595)
                     /locus_tag="IVK78_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6376032.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IVK78_002634"
     CDS             4799..5095
                     /locus_tag="IVK78_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6376033.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IVK78_002635"
     CDS             5287..5907
                     /locus_tag="IVK78_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP6376034.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
BASE COUNT         1750 a         1225 c         1095 g         1902 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tg
//
LOCUS       DACCCM010000116         5905 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCCM010000116 DACCCM010000000
VERSION     DACCCM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.121"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(95..667)
                     /locus_tag="IVK78_002636"
     CDS             complement(95..667)
                     /locus_tag="IVK78_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6376035.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVK78_002637"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVK78_002637"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6376036.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IVK78_002638"
     CDS             complement(1352..1867)
                     /locus_tag="IVK78_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP6376037.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IVK78_002639"
     CDS             complement(1869..2153)
                     /locus_tag="IVK78_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6376038.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IVK78_002640"
     CDS             complement(2187..3515)
                     /locus_tag="IVK78_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6376039.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IVK78_002641"
     CDS             complement(3529..3984)
                     /locus_tag="IVK78_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6376040.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5852)
                     /locus_tag="IVK78_002642"
     CDS             complement(3987..5852)
                     /locus_tag="IVK78_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6376041.1"
                     /translation="MAVSTKTIYQDIELLEDYVVNYQVTIERLPSLGIKLIGDRKNVE
                     HLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRII
                     NKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSK
                     VISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRY
                     METYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLI
                     ERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVW
                     YAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVR
                     RFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIME
                     AYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEECLDLMISELE
                     ANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALKNAINWGDRK
                     VKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2003 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatt
//
LOCUS       DACCCM010000117         5529 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCCM010000117 DACCCM010000000
VERSION     DACCCM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.122"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            277..1125
                     /locus_tag="IVK78_002643"
     CDS             277..1125
                     /locus_tag="IVK78_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376042.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1296..1637)
                     /locus_tag="IVK78_002644"
     CDS             complement(1296..1637)
                     /locus_tag="IVK78_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP6376043.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1723..2430
                     /locus_tag="IVK78_002645"
     CDS             1723..2430
                     /locus_tag="IVK78_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6376044.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2417..2740
                     /locus_tag="IVK78_002646"
     CDS             2417..2740
                     /locus_tag="IVK78_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6376045.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2829..3863)
                     /locus_tag="IVK78_002647"
     CDS             complement(2829..3863)
                     /locus_tag="IVK78_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6376046.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
     gene            complement(4310..5293)
                     /locus_tag="IVK78_002648"
     CDS             complement(4310..5293)
                     /locus_tag="IVK78_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP6376047.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT         1623 a         1147 c          987 g         1772 t
ORIGIN      
        1 tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct aacaaaatca
       61 cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa cacgctattc
      121 ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt gacaattcga
      181 gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt ttctttatac
      241 taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct tgagtatttt
      301 cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt ccgtcatttg
      361 aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta taaggtagtg
      421 atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat agaaggaaaa
      481 aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc
      541 aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg
      601 gagcttttaa cagagattct tatagaacat ccgcacttta tcgaacgctt caaagttaag
      661 atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca taaatacgat
      721 catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag
      781 ttcatccaag aagcagttga atcgcttatt cagcaaaatg cctatcttcc cgcattcgaa
      841 ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga ggatgaacga
      901 acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc agaagcacct
      961 catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc
     1021 tcttattcaa aacgctactc tataaaaatc ctgaaagaag actttcggga aaaagaattt
     1081 tctaaccaaa agaaaaaaat agataaaaaa ctaaagaaac ggtaaagaaa gacgatttca
     1141 atgaaaaata gcaagtaaac gataacaacc atcctaaatc cacaagaaat acagcagacc
     1201 ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag aagacttaac gcgtacccaa
     1261 tattagtcat ttgactatta ggtacgcgtt ttgttttata agtcatgaca aatttccttc
     1321 gttgctggat agattttccg gcattgttga tagattttat gatacttttc tacattttgt
     1381 aagagaggtt ccactgcatc tgtatattgg ataaaattct gacaacaact ttcaggtgtg
     1441 tcgaaccacc ctaaccctag tgcagcaatc attgttgcgc ctaatgcagg tccttcctcc
     1501 acacttaaac aatagaccgt tgcatcgaaa atatcagctt ggatttgtag ccatgcaaga
     1561 tttttcgcgc ctcctcccac cgaaacaata cgtgtaaatc tgcgacccgc tagttcttta
     1621 cgtttcaatc ccaccattga caaatgctca ttaaaaattt agaatattca caagtttaac
     1681 gacaattatg aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat
     1741 ataaaaactg cgatcaaaga cacgttacct accgttttcg gttatatcgg tattggactt
     1801 gcatttggta tcgttgggaa agctgccgga tttcatccac tagtcgtcac gttgatgtcc
     1861 ctgctggtct atgctggttc tgcccaattt atcacagtca gcatgcttgc tagtcacagc
     1921 ccattgcttt ccatcgtttt ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt
     1981 atgacgattg ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca
     2041 ttgctgacag atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag
     2101 ttaaactttc gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct
     2161 tctcttgttg gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat
     2221 ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac
     2281 acctctaaac gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga
     2341 ctgattttta tcccaagcaa cttagtgatt gtagttgtaa cactgattgg gtgcggattg
     2401 ggggtgtgga tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac
     2461 tttagcaact tggctttcta gggtcctacc atttgtctta ctgaaaaaat tcgatttacc
     2521 gcaaccgttg cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt
     2581 cagcagtcta tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt
     2641 ggcttcagtt cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt
     2701 ggcaggaatt ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat
     2761 ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt
     2821 attcttgctt atccgttgaa cggaccacta acaacgactt ttcctaacat atgtcctgtt
     2881 tctacgtctt tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg
     2941 ctcaatgtac tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct
     3001 ccttgcgttt cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag
     3061 ctgatagatt ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt
     3121 cccacatgtc caaatggtcg gacaaggtgc ttgatttgat caaagtagcg ggtaatatcg
     3181 aataatacag cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag
     3241 tcgtgatgat aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc
     3301 ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa
     3361 ccgacgcccc cggatccgtt gatcactaag atttttttcc cgcgattggc attttcttct
     3421 ggagttagac caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag
     3481 gctgcagctt cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaact
     3541 agttggtatt cttgattgct tcctgctctt gttgtacctg cgtaaaaaac gcgatcgccg
     3601 acagaaaatt tttgcacttc gtcacctaca gcaacaactt tcccgactcc atcaaaacct
     3661 aagatacgta aagcatttct tatcccgtta tcttggcgaa gtttcgtatc gacaggatta
     3721 acagatactg cgttgacttt taccagaaga tcccgtgaac tgggtacagg tatctgttct
     3781 ttttcatcaa taaagctttt tggatcatcg atcggcaacc cttcataaaa accgactacc
     3841 ttcattaatt ccttttctga catacgaatt cctccttagc ttcatcaagt tggagcatcc
     3901 atacagtgtt catttcaata tatatagtat gctatttgtt ccaccttttt tattcatcat
     3961 aattattctc ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga
     4021 caagactttt gtcacaacct cttataaacg gtgttctcct accaactctt cggtcacaag
     4081 tcaaaatatt catcaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt
     4141 taaacggcag gctcacaacc tctgatataa acggtgttca atcaactgac cctcggtatt
     4201 agctcaaaaa acgacaaaat atgaagagct atttttgttt ggctgttctt attacttgat
     4261 acaggacagc tttttcacga cctctaattc acggtgttca agcaagaaat cattgttcac
     4321 tgaaaagcgt tggatgttct ttttcaaagg tctctaagat ctctcgtact tcatcagtag
     4381 atttcgtatt catcaactga actcgcaagt catttgctcc tggaaatcct ttgatataga
     4441 ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat gatttctgaa taatgatctt
     4501 gcagatctag ttgcagacga agtagtccga tcagttcttt ttctgtatgt tccctcgggt
     4561 ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc aatcatgatg ccatcaacgc
     4621 catatttctc ggccaattct tctcctgtct ggcgatcggg gatatctcca ttgatcgtga
     4681 ttagtgtttg tggagcgatt tggtcacgta aagcaatgat tttaggaatg acttcccaat
     4741 gtgcatctac tttactcatc tctttacgcg tacgcaaatg gacagaaaga ttggctatat
     4801 cttgatgaag taggtgactg atccattctt ccatctcaga catttccgta tagccgatac
     4861 gcgtcttgac gctgacaggc aaaccgccag ctttagctgc ttcaatcaat tgtgccgcta
     4921 cttctggacg aaggatcaag ccactgcctt ttccacgttc tgctacatta gggactgggc
     4981 agcccatgtt gatatcgatt ccttggaagc ccatttctgc catagcaatg ctcatttctc
     5041 taaaaaattc tggcttgtct ccccaaatat gggcaaccat cggctgttca tcttctgtaa
     5101 atgttaatct tcctcgtaca ctgtcttttc catctggatg acagaaactg tctgagtttg
     5161 taaactcagt aaagaagaca tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa
     5221 catctgttac atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa
     5281 aattagtagt catttatttg ttactctcca ttctatcaag cagtgaagtc gcaatctttc
     5341 tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag
     5401 atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc ttatcacagt ccattttccg
     5461 gaataaatgg tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag
     5521 gaggagcta
//
LOCUS       DACCCM010000118         5501 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCCM010000118 DACCCM010000000
VERSION     DACCCM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.123"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            168..1142
                     /locus_tag="IVK78_002649"
     CDS             168..1142
                     /locus_tag="IVK78_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6376048.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1124..1585
                     /locus_tag="IVK78_002650"
     CDS             1124..1585
                     /locus_tag="IVK78_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6376049.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1635..2099
                     /locus_tag="IVK78_002651"
     CDS             1635..2099
                     /locus_tag="IVK78_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6376050.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2113..2862
                     /locus_tag="IVK78_002652"
     CDS             2113..2862
                     /locus_tag="IVK78_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6376051.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2862..3692
                     /locus_tag="IVK78_002653"
     CDS             2862..3692
                     /locus_tag="IVK78_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6376052.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3692..4339
                     /locus_tag="IVK78_002654"
     CDS             3692..4339
                     /locus_tag="IVK78_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6376053.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4437..5213)
                     /locus_tag="IVK78_002655"
     CDS             complement(4437..5213)
                     /locus_tag="IVK78_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6376054.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5295..5438
                     /locus_tag="IVK78_002656"
                     /pseudo
     CDS             5295..5438
                     /locus_tag="IVK78_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1882 a          747 c          979 g         1893 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggcatttt attatataaa attgttataa
       61 aaaatttttt ttgaaaaata gtattgcaaa ataaagcaat ttcatagata ataggtatcg
      121 taaagttata taactttata atacctatta gagtgaggga taaagaaatg agacaactac
      181 acaaagaaca gattgaaaca gcaatggcag cgttaaagga aaagacaatt gatcatgtat
      241 attttacagc atgtgggggt tcacaagccg ttttaatggc tggtcaatat ctatttgata
      301 aagaattaac tgtaccttgt catgtttata ctgctaacga atttaactat gctataccaa
      361 aaggattatc tgaaaattct ttagttatca cttgttccca ttctggcaca acaccagaaa
      421 ctgtagaagc aacaaaaaaa gcaagagaat ttggagcaat gactatttgt ttatctaatg
      481 aagaaggatc tcctctatgg gaagcagctg aatatcctgt tcactatgat tggggtaaag
      541 atgttgatgc tagtgattta aataaaggaa ttctttatgg tttattattt aatattttac
      601 ttgttttaga tggctctaaa aaatgggaaa cttgcttgaa tgaatttgat aagttaaatg
      661 atcttgttat tgctgaaaag agcaaatttg aaataactgc taaagaatgg ggtaaaaacc
      721 aaaaacgaga taaaattatt tacacaattg gtagcggtat taattacgga gaaatttact
      781 ccacagcagc ttgctggttc atggaaatgc aatggattaa ttctaatgct attcattctg
      841 gggaattttt ccatggtcct tttgaaatca ctgattatga tgtaccattc atcttagtga
      901 aaagtattgg agatacaaga tttttagatc aacgggtaga agacttcact aaaaaattca
      961 gtaataaatt aatggtttta gaccaagctg attttgactt agaatctata gatagtgtag
     1021 cgaaagaata tattgcagct gtacttagtg gcgttgttat tcgccaaatg gtagaagcga
     1081 ttgcttttga aagaggccat tccttagatg ttagacgcta tatgtggcaa atgaaatatt
     1141 aattaatagg aggttgcaaa agatggcaaa tcaattattt cacattgtga ttacaacaca
     1201 ttctaccctt tgtgagggtt atttaaaggc aacaaaatta attttaatga atgacgccac
     1261 aggtattagt gctgttccat ttaaagaaaa tactagtcca gatgattttg aggagagtat
     1321 gaaagaaata attagtcagc atcatttgca acctctaatt attttaacgg atttaattgg
     1381 aggaacacct aataatgttt ctattaaaca gttgaacaac aatagaattc aaattattgc
     1441 tgggattaat ttacctcttt tactggagtt attaatggcc cagtcagctg gtaaatctat
     1501 agaagaaata gattttaagg aattactgaa taactctcaa aaaagtatgg ttcatattaa
     1561 ttcgctttta aaagatgtca gttaaggtgg caatttctat tctataaatt ttaaaaattg
     1621 aaggaggaaa tagaatgatt aaattagtgc gtgtagacca cagattactt catggacaag
     1681 taatattttc ttggactaaa cagtatgata ttaaccatat tattgtggct gataacaaag
     1741 ttccaggaga tccaatgtct gtgatggctt tgacaattgc taaacctgct gattgcaaat
     1801 tagacattgt taaaattagt gatgttaaag ggattatgga aacatcccca aatgataatt
     1861 acatgatttt aattaaagga cctaaggaag cttttgaatt agttcaaaat ttaccagaaa
     1921 ttaaagaaat taattttggc ggtgtgtcta agaaaagcga gtccaaacaa tatggaaagg
     1981 cagtcttttt aactcctgaa gaattagaag ccacaaaaaa aattcttgat caagaagtga
     2041 aaatatttgt gcaacaagtc ccatcttcaa gtattgaaaa tgtagatttt agtaagtaag
     2101 gggggaaaat aaatgttatt aaaagccatt ttattaggtt tagttggtgt ggtagctgtt
     2161 attgactcca ggttaattgg tcgacaaaat attggtagac cactaatttt gagtacactt
     2221 gccggacttg tgctaggtga cttgcataca gggattgttc ttggggcatc tttagaatta
     2281 atttctatgg gattcgtctc aattggggcg gctggacctc ctaatatgca acttggtagt
     2341 attattgcca ctgcttttgc aattttaact aatagtagta cagaaacagc cttaacaata
     2401 gctatcccag tggctgtagc tggtgaattt atgagtatta tcatgcgtat gataattgct
     2461 caatttggac atgctgcaga taaagccatt gaaaataata aatttagaaa agctcaaatt
     2521 attcatattt attggtcatt tatttttaat gcttttgttt actttattcc aatcttttta
     2581 acagtatatt ttggtgctga cgttgtggca gatttagtag ataaaattcc agaaatgatt
     2641 acagacgcct tgactgtctc tggaaatatg ctttccgcat tagggtttgc aatgctatta
     2701 agcacaatgt tgagtaaaaa attatatcct tactttattt ttggattctt tattgttgcc
     2761 tattcaggtc tttcactaat tggcgtgaca atttttgcag ctctgattgc ctttgtaatg
     2821 gatcaagtta aatatggaaa aagagaagag gcgcatggtt aatggaaaat agtacagaaa
     2881 aaaaacgttt aatcacaaaa aaagatcttc ataagatgtg tcttcgttca tggacaatca
     2941 tgtgttcgtg gaattatgaa agacaaatgc atatgggatt tatgtatggg atggcgccaa
     3001 ttttaaataa agtttataaa aatgatgaag agaaaaaaag agaagcttat actcgtcata
     3061 tggcattttt taactgtacg cctcaaatga ctccatttat tatgggactt tgtgcttcta
     3121 tggaagaaca aaatgctcaa gcagaagaag gaaactttaa tactgaatct atctccatga
     3181 ttaaaaccag tttaatgggt ccttttgctg gaattggtga ttcatttttc caaggtactt
     3241 taagaattat tacatttggg attggattgt cttttgctaa gcaaggtagt attttaggac
     3301 cgattttagc tgttggcctt tttgctatac cttctatttt agctgttttt tacggcggta
     3361 aattaggata tgagtctggc aataaatacc ttactaaatt gtatcaagaa ggtcttatgg
     3421 atcgagtaat gcaatttgct tctatcattg gtttggctgt agtaggtgga atggtggcta
     3481 gcatggtgcc aattgtcacc cctttaaaat tcactacagg gggaacagaa cttgttttac
     3541 aagacttact tgattccatt attcctggat tattaccact tgtatgtact ttgggaatat
     3601 ataaattgat taagaaaaat gttagtacaa atatgctctt attaggttgt attttggcag
     3661 ggtttgtttt aagtataata ggtatcttat aatgattaag gctctaattt ttgatatgga
     3721 cggagtttta attgatagcg agattatgta tcaaaagtgg gtatttaatt ttttaaaaca
     3781 agaaaattta agctatcctt tgtattctta tcaaaaaaag attggaacta cagcaagtat
     3841 ttttgatgat atagaaaagt acaatgaggg atgtaacagt ttagaattga agggaaaatt
     3901 tactaaatat tggaaatcgc aaaagattga ttacttggat atttttccta atatactaat
     3961 tgaacagctt gaatggttaa aaaagaaaaa aataaaaatg gcaattgctt cttccagtcc
     4021 aatggatact ataaatgaag ttgttaccac ttgtaaaata gagtcatatt ttgattgttt
     4081 aattagcgga agagatttgc cagaaagtaa acctaatcct actatattcc ttaaagcttc
     4141 agaacagctc agaattccag ttgaagaatg tctggtgata gaagattctt ataatggtat
     4201 aaaagctggt aaaagagcaa atatgaaggt tcttgcaatt aaagataaac gtttttctca
     4261 agatgtaagt ctagcagatg atgttattta cgatattaaa tatctgcgac aagaagtaca
     4321 ggtgaaattt ggaatataaa taagtttttt gatgtattta gagaatatta gtaaaaataa
     4381 aaatcatgcc caaatctaat caaaattgat tgctttgggt atgatttctt ttaatatcaa
     4441 tctattactt taggatctga atcattttta tgcaccgtat ctaaagtgat attttctgtg
     4501 acttcaataa cataactaat tctatcacaa cgaactgtgg attgagaata atgaaatggt
     4561 tgattatttt tatctttgcc aaccttttct aaaataaata gcggatctcc tactatacat
     4621 tgcaataagt cggaaagctc aggtgttgca gataaaccgt ttatttccat aatagagctc
     4681 gtagctttga catcatagtc atcttctaga agtttatata atgaagtata ttggctacct
     4741 ttttcaaata aagttggaaa tttttcttct gatgcgaaaa aattatcaat tcccatcggg
     4801 gcaccgtctt cccacattaa acgtttaata ttgtaaactc tatcagttac attgatttgt
     4861 aatttattag ctaaaaactc atcagccttt ataattttag aatgtaaaac agtatatcta
     4921 attttatgtc ctcgggtaga aagagaatcg ctaaatccat gaactccagc taattttaaa
     4981 ctttcttttt tttgagttac aaaggtccct ttccccctta atcgttcaac tgttccactt
     5041 tctgttaaaa gtttaaaagc ttttcgaatg gtaatcttac ttactccata aattttacat
     5101 aattcttctt ctgtaggtaa ttgctctcct ggaagatatt ttttttcacg aatatcgtta
     5161 cgtatagaag cttctacttg tagatataat ggtctattat cttgaaaatt catagtacac
     5221 tccctaaaaa gttatacttc attatatcac aatttattaa aataaaagat taagatataa
     5281 atataaatga actagtcttt gactaaccaa tgcccgcttt tgaaagaaaa caaattcatt
     5341 ctgttttaag taaaaatcct tatgttacca ctcattctga aggggatgag ccttatcgct
     5401 acttggttgt ggaaccaagc gataagacaa ttttataaat tcaatattaa ttaaaataaa
     5461 aacaaacaaa gtagattatt ttactttgtt tgttttttta t
//
LOCUS       DACCCM010000119         5313 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCCM010000119 DACCCM010000000
VERSION     DACCCM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.124"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..729
                     /locus_tag="IVK78_002657"
     CDS             <1..729
                     /locus_tag="IVK78_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376055.1"
                     /translation="GAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARMHKFSEL
                     DTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVS
                     RVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKTIVPVGSLWIDKKDG
                     KVLEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLE
                     TVSEVAKRKNEIYYEQQMSQKERV"
     gene            789..2249
                     /locus_tag="IVK78_002658"
     CDS             789..2249
                     /locus_tag="IVK78_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6376056.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYIIKDENKVERVEN
                     LHFETQEYKHHPIDYRPLTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNY
                     IVQDDRLDYAIVFPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTI
                     YEEVGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSE
                     VKKDWNDQLKHEIQQQAIRKQKRAMLFQQGRQQERE"
     gene            2260..4464
                     /gene="topB"
                     /locus_tag="IVK78_002659"
     CDS             2260..4464
                     /gene="topB"
                     /locus_tag="IVK78_002659"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6376057.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDEKWKQWSLAHLPIFPNQYHYHVPKDKKKQFNVVKRQLQSADT
                     VVIATDSDREGELIAWSIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQHKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAHVITQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKNLFGK
                     ESDGKEKEEEPLLPKVAKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIQVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGATLQEVKA
                     ASEKQEAARHLGICPKCQEQEVILYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            4710..5306
                     /locus_tag="IVK78_002660"
     CDS             4710..5306
                     /locus_tag="IVK78_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6376058.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
BASE COUNT         1937 a          922 c         1169 g         1285 t
ORIGIN      
        1 ggggcgtatg ttcgtaacga atttactggc gaagaagaaa tcgtcatgaa tactcgaaat
       61 actcctacgc aacatttagc tacaagtatt catgaattag cccatgcaag aatgcacaaa
      121 ttttcagaat tagataccgc aactaaagaa ttgcaggcag aaatgacttc ttatattgtg
      181 tgcaaacatt ttggaatgga cacgtcggag aaagctattc cttatattgc agcatggacg
      241 aagaatggtc agacgttgga tgataaggaa gctgcggaac gtgggaaaat catgaatgat
      301 gtcagccgtg tcgcaaatga attcattcaa accatttcta atgaaatcaa tcaatataga
      361 gaaagagaac cagagatcca accagcagaa gtgaagcagg agcataaaaa tactgtagaa
      421 acggcagaac aaaagactat tgtgcctgtt ggttcacttt ggatcgacaa gaaagacggg
      481 aaagtcctgg aagaagatac aggccgaacg ttacatttga aggatgcggc gtttattgaa
      541 gatccgcagc ataatcaagt gattctaaaa gggaaaagtt atgggaaaga gatccaaacg
      601 gctttacctt cagaagaatt tgcgctagga aatccattcc gattttcact agaaactgta
      661 tcagaagtgg ccaaacgaaa aaatgaaatc tattatgaac aacaaatgtc acaaaaagaa
      721 cgagtataaa acaagcaaga aagactatct gaaaaatgga tagtcttttt tcttttggag
      781 gtttgaaaat gacagagaaa aaaatgagtc ttattgatcg atgcaagcaa atggatattg
      841 tggattttgc tcgaaacaat ggtttagcgg tagtcaataa aggacgagac tatcgtttag
      901 aagaccatga ttcttttgtt ttcgatcgaa gaaaacagcg attttattgg aacagtcaag
      961 gtattggtgg ggatattatt gaattgactc aattgttttt tattgacgat tctattcaaa
     1021 acccaaagca acgttttaaa gcagcgctag attatattat aaaggacgaa aataaggtgg
     1081 aacgggtgga aaatcttcac tttgaaacac aagaatataa gcaccaccca attgattatc
     1141 gaccattgac agaaaaagga cgaagttact taaaagaaca acgaaaattc cctgaatggt
     1201 tgattgatta cggagaacaa caagggttga tcctggaaat gaagccacgc aatgaacgaa
     1261 aaaattatat tgtgcaagat gatcgcttag actatgcgat cgtttttccc tggaaagacc
     1321 gtcatacaga ccaaatatta ggggcaactt atcaagggac tatcgtagac tatgaacgtt
     1381 ttggaaaacg gggtgttgac aaacgaatag atataaatcc tacgcctaat cacgggttta
     1441 atgttcttct tggatctcct aagaatctga aattttttga gagtcccatt gatttactta
     1501 gttacgctgc tttaaatcgc gagacgatca aagacacgtg gcttgtttct atggatggac
     1561 tgaaggatgc agtagtgggg cactatgttc gagaagcggt gacagaattg gcgccgaaag
     1621 aagaatttcc agaatcgatt gagatttgtg tggataatga tcgagctgga cacgtattct
     1681 gggataaggt acaccgactt ggtgtgtcga atccatttac aggaaaagcg gtgtattttg
     1741 aaccgaatct tcccaacgat tggcaagtac cgagagaata tcaaacaatc tatgaagaag
     1801 tagggaaaga aatgagagtt gcgcctgaag caatcatggc tattcataaa atcgaaaaca
     1861 acctaacgga tactaaccaa atcgtatcgt tcggtgaaat ccagggagta tttgccaaaa
     1921 aacttgcgcc aaaagagaaa gcccaatcta ttgttgtaaa ggaaaaatgt atagaagcag
     1981 ctcagcagct aaaatcgtgt gaaaaatctg atggaacata cgattttgat cgtttttatc
     2041 gtggaaaagg gaaaattaat gaacaaattc aattttcatt gaaggccaaa cactattttg
     2101 aaggttataa aaatcatgaa cacgaatttg tttctgaagt gaaaaaagat tggaatgatc
     2161 aattgaagca tgagatccag caacaagcaa tcagaaagca aaaacgtgcg atgttgtttc
     2221 aacaaggcag acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca
     2281 gaaaaaccgt cacaagcgaa ggcttatgcc gatagctttt ctaaagctac ccgaaaagac
     2341 gggtattttg aaatccaaga tcggttgttt ccaggagaaa ctgtgatcac gtatggtttt
     2401 ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaaatggaa acaatggtca
     2461 ttagcgcatt tgcctatttt cccaaatcaa taccactatc acgttcctaa agataagaaa
     2521 aagcagttta atgtcgtcaa acgacagctg cagtcagctg atacagttgt gatcgcgact
     2581 gatagcgatc gtgaagggga gctgattgcc tggtcaatca ttcaacaagc aggcgccgat
     2641 caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag
     2701 ggattccagc agttacgaga cgctggagag acctatccaa aatttgagga agctcaagca
     2761 cgtcaaatcg ccgattggtt aattgggatg aacggcagcc cactgtatag tttactcttg
     2821 cagcataaag gcataccagg aagtttttcc ttaggcagag tccaaacccc aacgctctac
     2881 atgatttatc aattgcagga aaagattaga aacttccaaa aagaaccata ttttgaagga
     2941 aaagctcatg taatcactca aaatggtgca tttgatgcga agcttgatcc gaatgaaacc
     3001 caagcgacgc aagaagcatt tgaagcctat ttaaaagaaa aaggggttca attaggaaaa
     3061 cagccaggca tgattcgcca agtcgaaaca gagaaaaaaa gtgcggctag cccacgtctt
     3121 ttttctttat cgagccttca gtctaaaatg aatcaactca tgaaagctag tgccaaagac
     3181 acattggaag ctatgcaagg cttatatgaa gggaaatatc taagctatcc gcgaacagat
     3241 accccttata ttacggaagg ggaatatgcg tatctgttgg atcatttaga tgaatacaag
     3301 cattttttaa aggctgaatc aatcccaacc cctattcata cgccgaatag tcggtatgtg
     3361 aataacaaga aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca
     3421 gcggcttttg aacaactaag tccactgcag caggctattt atgaacaagt gttaaaaaca
     3481 acggtggcca tgtttgcgga aaagtatacc tatgaagaaa caacgatctt gacgcaagtt
     3541 cagcagcttc agttaaaagc aatcggaaaa gtgccggtag atctaggttg gaagaattta
     3601 tttggaaaag agagcgacgg aaaagagaag gaagaagagc cgctacttcc taaagtggca
     3661 aaaggagaaa tggtgacggt agatctacaa gtccttgaaa aagaaaccaa gccaccaaaa
     3721 ccttataccg aaggtacgtt gatcacggcg atgaaaacag cagggaaaac cgtggatagt
     3781 gaagaagcgc aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggca
     3841 aatattattg aaactttgaa acaaaaggaa tatatccagg tagagaaaaa taagctcgtg
     3901 gtgacaaata aagggatttt actttgccaa gccgtggaaa aagagccact gcttacgagc
     3961 gccgaaatga ccgccaaatg ggaaagctac ttgttaaaga ttggagaacg aaaaggaaca
     4021 caaaccactt ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtccca
     4081 ggacaaatcc aatccactga ttttggagca acgttacaag aagtgaaagc agccagtgaa
     4141 aagcaagaag ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtcata
     4201 ctttatcaaa aggtggcggc ttgcacgtct gaagcctgtg atttcaaact atggacaacg
     4261 attgccaaaa agaaacttac agcgacacag ctgaaagaga tcattcaaaa tggccgaaca
     4321 agccaaccag tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta
     4381 aaagaagact tcacaacgag ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa
     4441 agaacacgac gcacgacaaa ataatggagg gtttatctta tgcgaaatac aacacacaaa
     4501 caacaagaaa tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc
     4561 agcaaacgaa gactgcagca attaaagaac cagcaacgga ttcaaaaaga aatcgaatta
     4621 gatcgataaa atcttcctga ttgttgtata cgcataaact tatgttatac tatgattata
     4681 agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtatac aagctgctag
     4741 aagaatttaa tggaactttg gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa
     4801 cgatcaaacg tatggtagat cgaggggaac tggatcgaga gtatccaggt tttttcacct
     4861 tacctggtca atttcctgat gaattgttta tggcgcaaaa aaaatatgaa cgcggcatta
     4921 tttcacatat taccgcgtta gatttgtatg atttaacaga tatgatccct agacagatcg
     4981 atctaaccgt accttacggt tatcacgttt ctgaaaaagg attgaaggaa ttcgcggttg
     5041 agttacacta ttcaaaagcg gaatggtatg aattaggcaa gatcgaaata aagagtcgct
     5101 atgggaatcc tgttatagct tatgatccag aaagaacctt gtgtgacatt tggaatccct
     5161 ggtataacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg gagtcggaca
     5221 gaaaaaatct tagaaaattg aatgagtacc gacgaatttt gcctacagat aaaacgatgc
     5281 gctcttatat tatggcttta aattaaagga gaa
//
LOCUS       DACCCM010000120         5268 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCCM010000120 DACCCM010000000
VERSION     DACCCM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.125"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..465
                     /locus_tag="IVK78_002661"
     CDS             4..465
                     /locus_tag="IVK78_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6376059.1"
                     /translation="MTHSHKLMFEDRTIPLESYPTEQIIAEKLSATFDFLTDNSRGKD
                     FYDLYTIPKMEKIDEKTLKDSVRNTFTRRGKTDPLKIYYEKDMDVLKNSHELKDTWKK
                     YQKSNPYAASITYDETMDSISNLMEKVIHVEEKERKLAYQRHQQMNREMER"
     gene            468..599
                     /gene="ssb"
                     /locus_tag="IVK78_002662"
     CDS             468..599
                     /gene="ssb"
                     /locus_tag="IVK78_002662"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6376060.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            621..953
                     /gene="ssb"
                     /locus_tag="IVK78_002663"
     CDS             621..953
                     /gene="ssb"
                     /locus_tag="IVK78_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6376061.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            998..1231
                     /locus_tag="IVK78_002664"
     CDS             998..1231
                     /locus_tag="IVK78_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376062.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(1390..1512)
                     /locus_tag="IVK78_002665"
     CDS             complement(1390..1512)
                     /locus_tag="IVK78_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP6376063.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            1702..1926
                     /locus_tag="IVK78_002666"
     CDS             1702..1926
                     /locus_tag="IVK78_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376064.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            2369..2575
                     /locus_tag="IVK78_002667"
     CDS             2369..2575
                     /locus_tag="IVK78_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6376065.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            2575..2826
                     /locus_tag="IVK78_002668"
     CDS             2575..2826
                     /locus_tag="IVK78_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376066.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            3007..3279
                     /locus_tag="IVK78_002669"
     CDS             3007..3279
                     /locus_tag="IVK78_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376067.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            3724..3903
                     /locus_tag="IVK78_002670"
     CDS             3724..3903
                     /locus_tag="IVK78_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376068.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3962..4318
                     /locus_tag="IVK78_002671"
     CDS             3962..4318
                     /locus_tag="IVK78_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376069.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1869 a          845 c         1034 g         1520 t
ORIGIN      
        1 acgatcactc acagtcataa attgatgttt gaggatcgaa cgattccttt ggaatcctat
       61 cctacagaac aaattattgc tgaaaaatta agtgcaacct ttgatttttt aacagataat
      121 tcgagaggaa aagattttta tgatctctat actattccta aaatggagaa gatcgatgag
      181 aagaccttga aagatagtgt gagaaataca tttacacgtc gaggaaaaac tgatccgtta
      241 aagatatact atgaaaagga tatggatgta ctgaaaaata gtcacgagct aaaagatacc
      301 tggaagaaat atcaaaaaag caatccttat gctgcgtcga tcacgtacga tgagacaatg
      361 gattcgatta gtaatttgat ggaaaaagtg attcacgtcg aagaaaaaga gcgaaaacta
      421 gcttatcaaa gacaccaaca gatgaataga gaaatggaga gataaagatg attaataatg
      481 tgacattagt aggacgctta accaaagatc ctgatttacg ttataccgca agtggtactg
      541 cagtagcgac ttttacccta gccgtcaacc gaaatttcac gaatcaaaac ggcaattgag
      601 aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg gcgacattag
      661 ctaaaaaagg tagcttgatt ggtgtagttg ggcggattca aacgcggact tacgacaacc
      721 agcaaggaca acgtgtatat gtgactgaag ttgtagcaga taactttcaa ctattagaaa
      781 gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc gtccaccagt ccaagcaccg
      841 ccacctttga acaacggaat acagcgacgc ctaacaacaa tggattgaac gcttcacaga
      901 atccatttgg tggacaatca atcgatatta gcgatgatga tctaccgttt tagtttgaga
      961 aagaaggagt aagcatggca aaaaaattag tctgacgatg gatctattga tacccgtaat
     1021 tgcatttata gacgggattt ctttgggcgt cactgaaaat cctttggtaa atagaattgc
     1081 gaaaggatca ctatttcttg tattcgtgta tcttgtctgt tcttatcttt taaaaagagc
     1141 aggaaaggat aattcctttt ggcgattgac taaagtcaca gtagcagcga cagctgttat
     1201 acttacggct attctaccgc tttatatgta gagttaacgg gaaatcgttg aacttcttgc
     1261 gaaatattgc tataatgaac atgaaaaaga ggagttgcgc taacaacccc tcattaacag
     1321 caccgattaa agaacggtgg cagaatatta gttatttgga aataaccgct gacctgctaa
     1381 agtagacggt tatttctttt tgtcattttt aagcatattt acaatcaaac taatcaaagc
     1441 aatggtaaac ataccaaaac caagaatggt ttgaattgtc tcatatgcgg acaaaaggct
     1501 tactcctttc atagatttta gcacttacat tcataagcac caccaccttt ccagaagtcg
     1561 ccaccgtctt caaactttac tgtcttgact attatagcat atctaaaatc gaataaaaat
     1621 tctcaatttt agctgttttt gctataattt tagcgaggtg atcgtgatgg aacaatatat
     1681 tggtttactc gataaattag ggtgatcaag acacagccat tgcttgtgcg atttacatta
     1741 cgcacgagtc atgaaccaat caattgtgta gtggccaata tagaaatagc ggataaattg
     1801 ctgattttgg aagaaggcaa gtatactatt tcagtgacag gtcacttcaa taaacgaaag
     1861 caactagtca ttgaatcaat gcaaattaga aaccctgatc attttacgcg atcgatgagc
     1921 atataaaaga agagctgcag tcctaaatga ggattgtagc tctttttgtt ttacatagaa
     1981 acagtaaaaa tgaaaaggcc tgtttaggaa ggcacaagcg ccttctcaat tgaaagacct
     2041 ttctctcact ccaaccgcca ccgccgttcg agaagaaagg aagtgaacgc tacgcgttca
     2101 ctaagataaa gggtcttgca tagcaagaca aaatagaaaa aatttcctct caccaaaagg
     2161 ttcgctcctg ttcgttatct ctttattgat ttgtcagacc ggcaacaagt tgccttttat
     2221 caccaaaaag cgacaaggac aagggtaaat ggcacgctgc gcgcccttgt cttgtcgctt
     2281 ttcgttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa
     2341 cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc
     2401 cttcagcgaa ttattcccaa actatcatgc accacatact gtcgaatgcg attgtggaga
     2461 aagagcgaaa tgtatcaaaa gctattgttt gtatcaatgt ccaacatgcg ggaaaaaata
     2521 taccttgtct tatggcgatt atattttaat tgatgagaaa ggaaagaaac gctgatgaaa
     2581 acacaacaca aattattgac tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc
     2641 atttctgaaa aagtagcaga agttttactt attccgcggt actggacgat agaaacccct
     2701 ttagtagagg atgcaccgac tatgctattt gaagacctag tgaagattgc aggatatgaa
     2761 ctgcattatc aagaaaactt ggaaacttat gaaaacgaat attggttaac caaattagag
     2821 aactaaaaag gagaaaaaaa tatggctcaa aattactatg acgaatttgt caaattacct
     2881 ttggataaaa tggcacaaaa aatggaggat atgacgttcc tatacaacga aacacgtgtt
     2941 ccaaaaaaac attataaaga aaaattgtcg gtagctgtgg aagaaatgat tgaataaggt
     3001 gtcgaaatga atcttattgc gacgtactat cgaacactgg aagaactgaa aaagcaaaat
     3061 gcaaaatggt ttttccaagc cttactatgc ttggaagtag gtgtcaaacc aagcactatt
     3121 aaaccttctg aataccaagc tttagaacta acgtatgcga aatttatcga aacgaaaaaa
     3181 gccaagactg tttcaagtga atggttagat tattttgaaa atatcaataa gtatggcgca
     3241 tattacacca tgaaaaagga agacaacgaa aatgaataag gtgatttatc aactaacaac
     3301 ggattccata tcaacgattt gaaagaaaaa aaggtcaata atcatttata ccgaaaagtg
     3361 agaatgtgtg aaacaaggac aaaaaacgcc tagtaactaa ggaaatggtt cttatttcgc
     3421 accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataaacggg caaagcccga
     3481 taacttaaga gaaagaaggt gatccatttg aacaagatta ttcccttatt gttagtactg
     3541 tttgttggcg aaatacttat gcgagatagt gcagctgtac agttaaacct cgctgtattt
     3601 ttggtaatac tggtgttttg tttgatcttt tggcggtttg tattagtcct cgtgagtcta
     3661 ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa ggagtgagta
     3721 ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga aaagagacaa
     3781 aaagaattgc agcaactaaa gcagaagtat ggcaaaagtg aatctaagat tatgtgtatt
     3841 gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atgtaccagc gctaaaaaag
     3901 tgacgtcact ttttaatttg atctatataa gtgatatcac ttttaccaag gagggaatta
     3961 aatgttaaaa actaaaaaga attcgtacaa attaaagctt agtatactat ctattatttg
     4021 tcttttaggc atgataatta gtggtcattt gtatagagtg gcatatacgc agtttattga
     4081 gtacaaggtt cactctttag gattagctgg ggatcgatac gttgaatatg tccattattc
     4141 attaaaatgt tcttcatata ttcttctttg gcttattttt tcattgtttg aattgctaaa
     4201 tttaatagca atatcttcat atcacttaga taagctaaat accaaatcta gccgttataa
     4261 cgaagtgttt aaggaaaatg tgaagttaga aagtgactta aacaagctaa aaaaataaaa
     4321 agaagaaaac ataagttaac taaaattatt ttattaagaa tgatggaata taaggataag
     4381 gttaccaaca taacgatcat tacttctctg atatgctatg gacttctatc aatcagtatt
     4441 ttgtctatag attatggagt aactatactg caagccttag ttttggctat gcctatgtat
     4501 ctatctttgt ttatcatgca tgaaatatat aagctatttg ttttatcaaa aaatgtagaa
     4561 atacgggtaa atattttggt gaatagttat caaaaagaac tcaaagaaag aagacggtgg
     4621 cggtatttat tgaaaggatg aaaaagtagg atttgacata ctggtattca gaaagtgccg
     4681 tcacttttaa aacaatggaa aaggtggaga taagatgaag aaaaaacaaa ctaaactatc
     4741 attagcagat atttaacaca gttaaccgca actgaagatg gtgttgttga atttgaacaa
     4801 tctgaaattg cggtagtaga tgatcgttac cttaaaatgc cttacttctt tgaccaagca
     4861 aaggtcattt gcctttgtca ctacgatggg gtacgtgatt ttaggcgtta aaaagtagtg
     4921 attatatcaa ctttacgagt aaatataaat ttagacatga aactgaatta tccaatggaa
     4981 aatcatggtt tataatctag aaaaattata gtaatgattg gattatatat attctttcgt
     5041 attttcgtac aactgtagct tgtcaaaatt gagaaattta accgattagg tactttacat
     5101 cgttatttgg gtaatttcta aaacatgaag cttaccaacg aataaactga gactatctta
     5161 ttaattgatc aatataagaa aatatctttg aggagatgat gtactatgtt ttcgtctatc
     5221 cacgattggt taggtggtct tttcggcggt ggaggtaaag gtttttaa
//
LOCUS       DACCCM010000121         5257 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCCM010000121 DACCCM010000000
VERSION     DACCCM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.126"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1241..2260
                     /locus_tag="IVK78_002672"
     CDS             1241..2260
                     /locus_tag="IVK78_002672"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAP6376070.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQE
                     MIKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSF
                     IRNQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGH
                     FNSFFYSNQSPTFKHHHIKKNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCY
                     DNPKYKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQ
                     KLYLYGGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKL
                     KIMIV"
     gene            complement(2762..3178)
                     /locus_tag="IVK78_002673"
     CDS             complement(2762..3178)
                     /locus_tag="IVK78_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376071.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(3180..3461)
                     /locus_tag="IVK78_002674"
     CDS             complement(3180..3461)
                     /locus_tag="IVK78_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376072.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(3837..4361)
                     /locus_tag="IVK78_002675"
     CDS             complement(3837..4361)
                     /locus_tag="IVK78_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6376073.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            complement(4378..5244)
                     /locus_tag="IVK78_002676"
     CDS             complement(4378..5244)
                     /locus_tag="IVK78_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6376074.1"
                     /translation="MRDVGDKKVQFTFEQLKDLSNYKATANVRFIDDLETTYDKLMDL
                     RFGRRSADGLQRERFVLFNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQF
                     NELESSYSKTMFRLLKQYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQEL
                     TAIFKGLTIQKRYGKGRGKPVIGYQFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQ
                     VQSEKAPTSLQEKIKEQKRKLELLQQLEKLEREQNTQNKENNAQKSEINSDIPSELKK
                     DLLDNLQNLFKD"
BASE COUNT         1638 a          815 c          895 g         1909 t
ORIGIN      
        1 atagagaaga ataaattcat ttctactgac gtaaatttgc gtaaaggaat cgtatttaat
       61 tcatggtggt atttcacaat ttcatttgcc ataaaatcct ccttcaagat taactactta
      121 acaaaattat aaagagcttt ctaatagggg tcaagcccct aattaaagac ccctaaaaac
      181 agacaaaaag acccccaaaa acagacaaaa agacccctaa aaacagacaa aaagacccct
      241 aaaaacagac aaaaagaccc ccaaaagatt ctgcacacct tgggagagta agcatacagc
      301 cgacggtaaa gaggtttaaa aagaggttta aaaagagatt ataaaagagg agaggggcaa
      361 aatttcccgc ctaaaaattt tctgcttttt tagccaaaca ggttcgcgaa ttttactaaa
      421 ctgggaaact tgctccctct ggtcgctaaa aaggtgtgag ggaaaaacac cctcacattg
      481 tacctgcgca gtcgcctaaa ggctcctttg ctaaaaacgt tgtttaaaga gattttttta
      541 gaggctctgc gcgctcgttt aagccgtttt aagacagttt agtatggata acccttatct
      601 tgttcttaaa gcaccttaca ggcggtcagg gcaagcccta acaaccctta aagagcataa
      661 aaccatccta aaattcgatt taatctgtct tatctatgtt cttgtatatt ttatatacct
      721 tgggtagtaa aacgtctgaa aacgcaaata agagcgtatg aaacaaaaca ataagcaacc
      781 gattcagcag aattccggag gagccatttt cgtacgttag caaaaaagtg ctgttgctaa
      841 aagctgcttg cggtgcggag cgaacaaggt ctcctagagc gagacgtgta caaacaagct
      901 tacaaattcg tgaaaggcac cggttttgcg ccatttccaa gcaaaaaaag gtctatcgag
      961 caagctcgat agaccgatcg catttagccc cccgcattct aacagggggg gaataatgtg
     1021 aaaaattatg gtagtaatgc ttgatatata ggcatttctt ttggttgcac acttaaaaaa
     1081 aatcacgatt ttgacaacta aatttttaaa taaaaaagct ttacagttta attcaaatgt
     1141 gttttaatat atctatcagt acttgccacg cctctgattt aatatcatgc gcattatggt
     1201 agggcttttt ttttatattt ttttaagaaa gaagattctt atgaagacat caaatagaaa
     1261 atataaagtt aaaacagtta atcatagaat tccacctaaa aaggcattat gtgtaattaa
     1321 taattccaaa gtaattaatc aagagttaaa accatattta tccgagcaag aaatgataaa
     1381 aaaaataagt aaagatattt ttttaacaaa ttccgataca attttattag aaaagttttt
     1441 aaagaaaaat agttattttc gatttagtat ttatgttaaa ttaatgaaaa atatagataa
     1501 agttactatt ctagatgtta ttgaaacgta caaattagat tcattcattc gtaatcaatt
     1561 gcattatttt gtgaatcaaa tagaaatttt ttggaaaaaa tctctttcag ataatatgtg
     1621 cgtttcttat gaagaaacta gcatatttcc gaagaatcag tgttatttgg ataaaaatat
     1681 ttattcagat ttgaaatggg ctgaagatat aattggtcat tttaatagtt ttttttattc
     1741 aaatcaaagt cccactttta aacatcatca tataaaaaaa aatcattgtt taccaatatg
     1801 ggctttattt gaagaaataa catttggtag tttaacaaca tttattaatc aacttaatac
     1861 agagtattat aataattggg tcatgagctg ttatgataat cctaaataca aaaaagctat
     1921 acgaagttgg gcaaacgtaa ttagagctta tagaaataag atagctcatc attcaagaat
     1981 atatgcattt aaagttactg acgctccaac aatcataaaa aatgattcca aaagttattt
     2041 tcctgaaaat aacttaaaag aaacccaaaa gttatatttg tatggaggat tatacgttat
     2101 aaaacatctg ttaatatatg aagatagcat gacacaaact ttatggaata aatttctaat
     2161 agatttagaa aaaaagatcg ataaaatccc ttccttagaa aaatccaatt atggttttcc
     2221 ggaaaattgg agaaacaagt taaaaataat gattgtttaa ttgttattta aattgttagt
     2281 agacataatg tacatactac caagtagccc ctggatcaac cgatccaggg gaagactttg
     2341 tgattttcaa ttggtctatc taaaacatct ttttaaaagc ttgattcttc gctgttggct
     2401 tttttcgatt ctttgtgaat attcagtgat tattttttta ctacttgtta ttttccgagg
     2461 gatttgttaa aagtttaata gcgtttctca gtaagggacg tcgttttttc attttcctat
     2521 gtatggactg ctcgaacgag cagccttttt ttattccagg gaacgtccta ttctttgagt
     2581 ttccatgcta ttgaataacc gggacttagg gattagggca gcgccccaaa atgaaaaaag
     2641 aaaaatccgc ttctgcgaat tcttcttttt tttcgcaagt tggtaccaac ttgcaaatct
     2701 tgctggaaga ccgatcccta aaaagaaatc gaggaatttt tttgtttttc ggtttctttt
     2761 tttatctttt tagaaagttt gttgggtcaa aatttccaga agtaaaggtt tgattggttt
     2821 ctttttgacg atttttgggt tgatttcgtc gggaaggatt gagttgattt tcaatttttt
     2881 ctttcacttc ttgatatact ttagaatttg taccgataag aacaccagta tcttttagat
     2941 ataaatcagc aaattctgcc atgatttctg tcatgataga aatttctttt cccatggcat
     3001 tgagttttgc ttggttactg gttgtggttg tattggtatc aggttcataa ctcatacata
     3061 aaaagcgaat ggcttctgaa taattgttaa cgtctggatt tttttcgatg atggaatcta
     3121 aaacgtcaac gtgactggga taaagggtta cgtgtttttg aacagaagtt tttctcatgt
     3181 tatattcctc cgataagtgt ttcaatgagt tggttgtttg tgtgaacata gtcatttagt
     3241 atgttcgtat tttctgcttg aatggcaata aatttttcga ataataaatc gaattgatct
     3301 tttttgtaat tcataagttc gtggtcaaaa tcgttttcga ttaaaatttt taggtattga
     3361 tttcgactcc attttttgcc cgttttggtt gttagttcct gacaacgttt atctattctt
     3421 gtaataattt tgggattgat atttcgaact aaaatgtcca tggaaatttt cctttcgtat
     3481 cagtgtataa tttatgtata tcatacaaat atagtatttg tatagctatt tttcgccaaa
     3541 acactataca tagcctagcc acaatggcaa aaaagtgata gcaaattttt ttctgagaaa
     3601 cgtgataggc acagcttatc tagcaccttt cgtaagaaag tgataccacg tttccttatg
     3661 ctctttcaaa attcagaaaa atcagctgta aaacgagtgc agtccgagta agattcaacg
     3721 aaaaaacggg gattggggcg ccaccaaaat atcgcaacat gggcccatgt tgctctgctc
     3781 gcaaaagaaa aaagaagaat aaaaccagca atttttatta atttaaattg ctggttttat
     3841 tgccagaatt gccaccattt tttctccgtc tttgattgtt tttctatcga ttctgtttct
     3901 tcagaaagag tcgttgattt attttccgaa gattcttccg tataagtcaa ctgtagttgt
     3961 tcctgcagtt tctcgatttg ttggttggct tgcagggtga gttgttgttg ttggtcgagt
     4021 aatttttgca tttgttttat ttgctcgtct ttttcttgta gttgcatatc tttttgattt
     4081 aactgttgtt ctaggagaga aaggacttct aattcgggaa cttggttggc gtttttgtgg
     4141 ttttcggttt tagttttttc gccaactagt tgttgatttg agttttcgtt ttcgttaaaa
     4201 aacattgatt ttatagcctt ttgtccggtt tcattaatca caaacttgtt tccgattttc
     4261 gaaaaccaaa gttttcgatt ttcgtcagaa acttttttgt taattgctgt tttactaact
     4321 cctaattctt cagcaagttc tttgattgtt ttttctgtca taaaatctaa aaacccctca
     4381 atctttgaat aagttttgaa gattgtctaa taagtctttt tttagttctg aaggaatatc
     4441 ggaatttatc tctgattttt gtgcattatt ttctttattt tgggtatttt gttctctttc
     4501 taacttttct aactgttgta aaagttccaa ttttcgcttt tgttctttga ttttttcttg
     4561 taatgaagta ggtgcttttt cagattgaac ttgttggtta gctagttcta atgctttgtt
     4621 ttgttcttct tttgttgggg catgtttgtt aaagtcgtct gattttttta tctcaggttt
     4681 aaatgtaaat tgatagccaa tgacaggctt tccccgtcct tttccgtatc tcttttggat
     4741 tgtcagacct ttgaagattg cggttagttc ttggcgaata ggctttataa tttttttatt
     4801 tatatcactt tctctataac ttttagggat atcaagtaat tcatgaaagt cttcttttga
     4861 aaaataggca attcctttgg ttctgtattg ttttaataat ctaaacatgg ttttggaata
     4921 actgctttct aattcattga attgttggag agaatatcga acccattctt caagattatt
     4981 aagtaagggt aaggctttct catggacttg tatttcagca aaaggaatat cagcttttcc
     5041 atcgatttta aattgattaa ataaaacaaa tcgttcacgt tgcaaaccgt cagcacttct
     5101 tctaccaaaa cgtaaatcca ttaatttgtc atacgttgtt tctaaatcat ctatgaatcg
     5161 aacatttgca gttgctttat aattgcttaa atcttttaat tgttcgaatg taaattgtac
     5221 ttttttatcg cctacgtcac gcattctaga tactata
//
LOCUS       DACCCM010000122         5256 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCCM010000122 DACCCM010000000
VERSION     DACCCM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.127"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(47..367)
                     /locus_tag="IVK78_002677"
     CDS             complement(47..367)
                     /locus_tag="IVK78_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6376075.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(515..2749)
                     /locus_tag="IVK78_002678"
     CDS             complement(515..2749)
                     /locus_tag="IVK78_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6376076.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQKAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3228..3494
                     /locus_tag="IVK78_002679"
     CDS             3228..3494
                     /locus_tag="IVK78_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP6376077.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3494..5221
                     /gene="ptsP"
                     /locus_tag="IVK78_002680"
     CDS             3494..5221
                     /gene="ptsP"
                     /locus_tag="IVK78_002680"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP6376078.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
BASE COUNT         1580 a          984 c         1057 g         1635 t
ORIGIN      
        1 ctttactttt gtcccacgct ctttaattat tatgttcaaa atttgtttac acggctaatg
       61 tagcgcctaa ttcatgtttt ggaatcgaga tttcaacaag gcctgccaat tcgattactt
      121 tgatttcatc ctcgtggtaa acggcgtctt tgattttatc taagaaataa tttttagcat
      181 cttcgatttc tttcggctgg attttacgtt tgtcattgat cagcagaatt tcattatgtt
      241 caggggcttc agtgtagaca atagtggtat ttccggctgt gtagaccttg actagatggg
      301 catcagtgtt ttcaagctgg tttaatacaa gacgggaatg gctgtttgtt acatttacca
      361 atttcatggc gctcacctct tttatgtatt tttaattgct tgtttctctt attataattt
      421 cttttttata aaatgaacag tattttgact tttctgaaaa aagaatgaga cggcatttct
      481 caaagaaata ccgtctcacg ctataaaact ggatttattc agccgtttct tcggctgatt
      541 ctttagccaa acgttctggt ttagaagtaa cgatgatttt tccatcttta tccagttttg
      601 ctttcaactc atggatagaa gggtgatcaa gataaaattc agcgatacca tcttcgattt
      661 gttcttggat cgtgcgtcga agaggacgag cacccataga tggatcatag cctaagtcaa
      721 ctaatttttc tttgacattc gtaggaactt cgatgtgaag ttgctgtgct gccagcattt
      781 ggttgacatc gtctagcatt agtgtgacga ttgtcatcaa gttttctttt gataatgcac
      841 tgaattcgat gatcccgtca aaacggttca gaaattctgg tgtaaagtag ttgtttaatt
      901 ggttcaatac agaacgagta acaccttcgc gtgccgcacc aaaaccaacg tttgcttcta
      961 ctttaccagt tccggcatta cttgtcataa tgatgatcgt atctttaaag ctgaccgttc
     1021 ttccttgtgc atctgtcaat cgaccatcat ctaagatttg caagaacata tgcaaaacat
     1081 ctggatgagc tttttctact tcatctaaca agacaagact gtaaggattt ctccgtactt
     1141 tttctgttaa ttgaccagct tcttcgtatc cgacataacc tggaggagaa ccaatcaatt
     1201 tagagacact atgtttttcc atatattcag acatatcgaa acgaatcatt gaatcttgtg
     1261 aaccgaatag ttcgtaggca agttgtttcg caagctctgt tttacctaca ccagttggtc
     1321 caacaaataa gaaggagccg attggacgat tctgtttgtt taatccaaca cggtttcggc
     1381 gaatcgcttt cgctactcga tctacagcgt tatcttgtcc gatgacatga gctttcaagt
     1441 catctgctaa gtttttcagt tgtgtctgtt ctttttcttt taattcaccc actggaatac
     1501 ctgtccgttg ttcaacgatt ttttccatat ctttttcggt aatcacaggt gtttcctctt
     1561 cagtgagctg acgttctttc attttttgaa gtttattgat ctgatcgcga taataagctg
     1621 ctttttcgaa atcttcttct cgagaagcta aaactttttg ttcttctgct ttttgcagtt
     1681 ttttctcgat tgttttagga tcaacgattt ggatcgtcaa gtttttctta gaacctgttt
     1741 catctagcaa atcgatggct ttatccggca agaagcgatc ttggacgtaa cgattggata
     1801 aagttgcggc tgcttcgatg gcctcatcag tatattttac atgatgatag tcttcataac
     1861 gtttttgcaa acctttcaaa atactgatcg tttcttcgac agttggttca tctacccgta
     1921 caggttgcat acgacgttct aaagcagcgt ctttttcgat gatgcggtac tcatttaagg
     1981 tagtagcacc aaccatttgc aattctcctc gagctaaagc tggttttaaa atattaccag
     2041 catccatatt tccatcacca gcagaaccag caccgactat ttcgtgaact tcatctatga
     2101 ataggatcac attttctgct tgacggatct cttcaatcaa tttttgcata cgttcttcaa
     2161 attgtcctcg tataccagta ccttggacta aagatacgac atctaaacga atgacttctt
     2221 tgtccattaa tttttgcggt acatcaccat ctacgatttt ttgtgctaaa ccttcaacaa
     2281 ctgctgtttt accaacacct ggttctccaa tcaagacagg attgttcttg gttcggcgat
     2341 ttaaaatttc aatgactcgt ttgatttcgt catcgcgtcc gatcacagga tcgatatcac
     2401 cttgtcgtgc ttgttctgtg atattgattc cgtattctcc caataaacct tctgtattgc
     2461 cttgaggtgg ctgaccacca ttgaagttgt tgtttccgcc aaattgtgtt ggtggtgttt
     2521 gctcataatt tccttgctgt tgcatttggc gagacatgga acggaaaaga tcgtccaaac
     2581 tgccaaatcc aaatggatca ttttgggcca tattagtcag acctccattt tgttgatttt
     2641 tgattttttg ataacagctc tgacagtaat cgagttgtgt ccgctgacca ttgacagtgg
     2701 catacaagtg aatcgttgcc tcattcttgc cgcagttttg acaaagcatg aatcattcac
     2761 gtcctttcac aaaaatgggt acatccatat tttacatctc gtttttcatg aggtaaaaga
     2821 ataaccccta gttttttaag agatttatga ggtagaaaag gtgaaatctc tattgaccat
     2881 tcctgacctt tgactcttat tatactaaaa ataaaaaggg aagcaagtaa ttcgtttcat
     2941 atgaaaacaa agagggtcaa aggaaagaat gataaacgtc aggagaagag aaagcgacaa
     3001 gaaaaaggaa ataaacacgt ttgttataaa atgtgaagaa aaggatgaaa aagaatccgg
     3061 aataatttga atacttttat ctgaaattga atgaaaactg ttgtatctaa cgaaaaaaaa
     3121 tgttaatctt tacagatgaa agggtggcat ttgttaggat tctctgaatt actaaaaaag
     3181 aaaaccctca cataaaaaca acactcactt aaaggagacc gattaatatg gaaaagaaag
     3241 aatttcacgt agtagcagaa actgggatcc acgctcgtcc agctacacta ttagtacaaa
     3301 cagcaagcaa atttaactct gatgtaaact tagagtataa aggtaaatca gtaaacttga
     3361 aatcaatcat gggcgttatg tctttaggtg taggtcaagg ttctgatgtt actattactg
     3421 cagaaggtgc tgacgaagct gatgcaatgg cagcaatcgt tgaaacaatg aagaaagaag
     3481 gcttatctga ataatggttg aaatgctaaa agggattgcc gctagtgacg gtgtagccgt
     3541 tgcaaaagct tacctgctag ttcaaccgga tttaacattc agtaaggcaa cagtagaaga
     3601 tactgcggct gaagaagctc gtttggatgc tgcattagct aaatctactg aagaattgca
     3661 gcaaattcgt gaaaaagcag cgcaaagctt aggtgaagca gaagcgcaag tatttgatgc
     3721 ccatttgatg gttctttcag atccagaaat ggtaggtcaa atcaagcaaa atattaaaga
     3781 caacagcgta aatgctgaat cagcgctaaa agaagtaacg gatatgtata tcggtatgtt
     3841 cgaagcaatg gaagataatg cttatatgca agagcgtgct gcagatattc gtgacgttgc
     3901 taaacgtatt ttagcgcact tattaggcgt tactttacca aatccatcaa tgatcaacga
     3961 agaagtggtc gttgttgcac atgacttgac tccaagtgat acagcacaat tagatcgcaa
     4021 ttttgtaaaa gcgtttgtaa cagacattgg tggacgtact tcacattctg ctatcatggc
     4081 acgttcttta gaaattccag caatcgttgg gacaaaagaa attactgcta aagtcaaaga
     4141 aggcgatatt ttggctgtca acgggatcga aggcgatgta atcatcgatc ctacagatga
     4201 acaaaaagct gaatttgaaa aagcaggtgc agattacgca gcacaaaaag cagaatggga
     4261 aaaattaaaa aatgcagaaa ctgtcacagc tgacggcaag cattttgaat tagctgcaaa
     4321 catcggaaca ccaaaagatc tagttggtgt ccataataat ggtggagaag cagttggtct
     4381 gtaccgtact gaattcctgt atatggattc tccagacttc ccaacagaag atgaccaata
     4441 tgaagcttac aaagctgtat tagaaggtat ggaaggaaaa cctgtagttg ttcgtacaat
     4501 ggacatcggt ggagataaag aactaccata tctgcaatta cctcatgaaa tgaatccatt
     4561 cttaggatat cgtgcattgc gtatcagttt atctgaacaa ggcgacgaaa tgttccgtac
     4621 acaaatgcgt gcattgcttc gtgcatctgt tcatggtaat ttacgtatca tgttccctat
     4681 ggtagctaca ctcaaagaat tccgcgcagc aaaagcgatc tttgaagaag aaaaacaaaa
     4741 attgataagt gaaggcaagg aagtatctga tacgatccaa gtcggcatca tgatcgagat
     4801 tccagcagca gcagtcttag cagacaagtt tgcaaaagaa gtagatttct tctctgtagg
     4861 tacgaacgat ttgattcaat acacaatggc agcagaccgt atgaacgaac gcgtttctta
     4921 cttgtaccaa ccatataacc catcaatttt acgtttgatc aaaaatgtaa ttgatgcagc
     4981 acacgctgaa ggaaaatggg caggtatgtg tggagaaatg gctggagatc aaacagctgt
     5041 tccattgcta gtaggtatgg gtctagatga gttctcaatg agtgcgacat ctattcttaa
     5101 aacgcgtagt ttaatgaaac gcttggacac agcgaaaatg gctgaacttg ctgatcgtgc
     5161 attgaaagaa tgcgatacga tggaagaagt cgttgaactt gttcatgaat atgtaaaata
     5221 aacaacgtat caaaagctgg tgtggcttgt aaaaga
//
LOCUS       DACCCM010000123         5228 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCCM010000123 DACCCM010000000
VERSION     DACCCM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.128"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..729
                     /locus_tag="IVK78_002681"
     CDS             <1..729
                     /locus_tag="IVK78_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376079.1"
                     /translation="GAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARMHKFSEL
                     DTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVS
                     RVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDG
                     KVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLE
                     TVSEVAKRKNEIYYEQQLSQKERV"
     gene            790..2259
                     /locus_tag="IVK78_002682"
     CDS             790..2259
                     /locus_tag="IVK78_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP6376080.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2270..4279
                     /gene="topB"
                     /locus_tag="IVK78_002683"
     CDS             2270..4279
                     /gene="topB"
                     /locus_tag="IVK78_002683"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6376081.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            4625..5221
                     /locus_tag="IVK78_002684"
     CDS             4625..5221
                     /locus_tag="IVK78_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6376082.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
BASE COUNT         1931 a          891 c         1135 g         1271 t
ORIGIN      
        1 ggggcgtatg ttcgtaacga atttactggc gaagaagaaa tcgtcatgaa tactcgaaat
       61 actcctacgc agcatttagc tacaagtatt catgaattag cccatgcaag aatgcacaaa
      121 ttttcagaat tagataccgc aactaaagaa ttgcaggcag aaatgacttc ttatattgtg
      181 tgcaaacatt ttggaatgga cacttcggag aaagctattc cttatattgc agcatggacg
      241 aagaatggtc agacgttgga tgataaggaa gcggcggaac gtgggaaaat catgaatgat
      301 gtcagccgtg tcgcaaatga attcattcaa accatttcta atgaaatcaa tcaatacaga
      361 gaaagagaac cagagatcca accagcagaa gtgaagcagg agcataaaaa tactgtagaa
      421 acggcagaga ataagaatat tgtgcccgtt ggttcacttt ggatcgacaa gaaagacggg
      481 aaagtaatgg aagaagatac gggtcgaacg ttacatttga aggatgcggc gtttattgaa
      541 gatccgcagc ataatcaagt gattttaaaa ggaaaaagtt atggaaaaga gattcaaacg
      601 gcgttacctt cagaagaatt tgcgctagga aatccattcc gattttcact tgaaacagtg
      661 tcagaagtgg ccaaacgaaa aaatgaaatc tattatgaac aacaattgtc acaaaaagaa
      721 cgcgtataaa acaagtaaaa aagactatct gaaaaacgga tagtcttttt tcttttggga
      781 ggtttgaaaa tgaccgaaaa aaaaatgagt cttattgatc gatgtaaaca gatcgatata
      841 gtggattttg ctcgaaacaa tggaatggct gtagtcaata aaggacgaga ctatcgctta
      901 gaagaccatg attcatttgt ttttgatcga agaaaacaac gtttttattg gaacagtcaa
      961 aatattagtg gcgacattat tgaattagct aaactttttt tcatagacaa agagatccaa
     1021 gactctaagc aacaatttaa agcagccaca gattttattc taaagaatga agacaaaaca
     1081 gaacgagtag aaaatcttca ttttgagaca gaaaagtaca aagatcaccc cgttgattat
     1141 caaccactca cagaaaaagg aaggaattat ctgaaggaag aacgaaaact accagagtgg
     1201 ctgattgatt atgcagaaaa agaagggttg attgccgaac taaagccgaa acatgaacgg
     1261 caaaattttt tagttggaga cgaccgttta gatcatgcgg ttgctttttt atggaaagat
     1321 ccacagacag gagagacagt aggggcaagt tatcaaggaa cgatcgtcga ttttaatcgt
     1381 ttcggtaaac ggggaacgta taaacacatt gacaagaatc ctacgccaaa tcacggattt
     1441 aatttaaaaa taggcgatcc gaagcattta aaattctttg aaagtagtat tgatctgtta
     1501 agttacgctg cattgaatcg ggagaaacta caagatgcct ggttagtttc gatggacggg
     1561 ttaaaacatc atgtaatcag tcattacgtt gaagagtcga tttctgaatt aagacgaaaa
     1621 caaacatttc ctcaatcgat tgaaatatgt gtggacaatg atcgtgcagg acatattttt
     1681 tatgaaaaag aacagatgaa gggaatcgtt gatcctttta ccaataagaa aatccgttgt
     1741 gagcgaggaa ttccaaatga ttggcaagta cctaaagaat acaaagctac ttatgaagca
     1801 gtcgcaaaag aaatgagtgt tgaacctgaa gcaattatgg ccatacacaa aactgaaact
     1861 aatttacagc tgacaaatca attggtgtct gctcatgatg ttcaatctac gtttggaaag
     1921 atgttggcga agggcgaacc agtagaaaca attgatttga aagaagcatg taccacggtc
     1981 gcgaaggaac tgaaagtatg tgaacgcgca gatggaacat acaactttga tcgtttttat
     2041 agtagaaaag ccaacataaa agacgttaat gcaggaatcc ttctttccta taaagcagaa
     2101 caatactaca aaggctataa aaagcatgaa catgaatttg tacctgaagt gaaaaaggat
     2161 tggaatgatc aattgaagca tgagatccag caacaagaaa tcagaaagca aaaacgtgcg
     2221 atgttgtttc aacaaggcag acaacaagaa agggagtgaa gtcgtcacta tgaaaaccgt
     2281 cattttagca gaaaaaccgt cacaagcgaa ggcttatgcc gatagctttt ctaaagctac
     2341 ccgaaaagat gggtattttg agatccaaga tcgattgttt acaggagaaa ctgtgatcac
     2401 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaaatggaa
     2461 acaatgggca ttagaacatt tgcctatttt ccccaatcaa taccactatc atgttcctaa
     2521 agataagaaa aagcagttta gtgtcgtcaa acgacagctg cagtcagctg atacgattgt
     2581 gatcgcgact gatagcgatc gtgaagggga gctgattgcc tggtcaatca ttcaacaagc
     2641 aggcgccaat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
     2701 catttatcag ggattccaac agttacgaga cgcagaagag acctatccaa aatttgaaga
     2761 agtacaagca cgtcagatcg ccgattggtt aattgggatg aacggtagcc cactgtatag
     2821 tttactcttg cagcaaaaag gcataccagg aagtttttct ttaggtagag tccaaacccc
     2881 aacactctat atgatttatc aattgcagga aaagattaga aacttccaaa aagaaccata
     2941 ttttgaagga aaagcccaag taatcgctca aaatggcgca tttgatgcga agcttgatcc
     3001 aaatgaaacc caagcgacac aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
     3061 attaggaaaa cagccaggta cgatccttca agtcgaaaca gagaaaaaaa gtgcggctag
     3121 cccacgtctt ttttctttat cgagccttca gtctaaaatg aatcaactca tgaaagctag
     3181 tgccaaagat actttggaag ccatgcaagg gttgtatgaa ggaaaatatc taagctatcc
     3241 gcgaacggat accccttata ttacggaagg ggaatatgcg tatctgttgg atcacttaga
     3301 cgaatacaag cattttttaa aagccgaagc gattccgact cctattcata caccgaatag
     3361 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacggt
     3421 tatgacagca gcggcttttg aacaactaag tccactgcag caggctattt atgaacaagt
     3481 gttaaaaaca acggtggcca tgtttgcgga aaagtatacc tatgaagaaa caacgatctt
     3541 gacgcaagtt cagcagcttc agttaaaggc aatcgaaaag gtgccgctag atctaggttg
     3601 gaagaagtta tttggaaaag agagcgacgg aaaagagaag gaagaagagc cgctacttcc
     3661 taaagtgaca aaaggagaaa tggtgacggt ggatctacaa gttcttgaaa aagaaaccaa
     3721 gccaccacaa ccttataccg aaggtacgtt gatcactgca atgaaaacag ctgggaaaac
     3781 cgtggatagt gaagaagcgc aatccatatt gaaagaagtg gaaggaatcg gtacagaagc
     3841 gacacgggcg aatattattg aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa
     3901 taagctcgtg gtgacgaata aagggatttt gctttgccaa gccgtggaaa aagagccgct
     3961 gcttacgagc gccgaaatga ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg
     4021 aaaaggaaca caaaccactt ttctagccaa tatccaaaaa tttgtctctc acttattgga
     4081 agtcgtacca gggcaaatcc aatccactga ttttggatcg acgttacaag aagtgaaagc
     4141 agccagtgaa aagcaagaag ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca
     4201 agaagtctta ctctatcaaa aggtggcagc ttgtacgtct gaagcctgtg attttaaaag
     4261 gtcagaaggg ttcatttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg
     4321 aattttctga aaagaaaaaa acgaacttca agaaaagaac acgccgcact gcaaaataat
     4381 ggagggttaa tcttatgcgt aataccacac acaaacaaca agaaatcgaa aatcaaaaag
     4441 ccaaaattcg ttcagaacag gaaaaaatgc gtgccagcaa acgaagactg cagcaattga
     4501 agaaccagca gaggattcaa aaagaaatcg aattagatcg ataaaatctt cctaactatt
     4561 gtatacgtat aaacttatgt tatactgttt ttataagttt atacgtatac aaaggaggga
     4621 gaaaatgaca aaagcaatgg tatacaagct gctagaagaa tttaatggaa ctttggcttt
     4681 gaaggatgcc aaaaaagcgg gcatttctcc tgtaacgatc aaacgtatgg tagatcgagg
     4741 ggagctggat cgagagtatc caggtttttt caccttacct ggtcaatttc ctgatgaatt
     4801 gtttatggcg caaaaaaaat atgaacgcgg cattatttca catattaccg cgttagattt
     4861 gtatgattta acagatatga tccctagaca gatcgatctg actgtacctt acggttatca
     4921 cgtttctgaa aaaggattga aggaattcgc agttgagcta cactatacaa aagcggaatg
     4981 gtatgaatta ggtaagattg aaataaaaag tcgctatggg aatcctatta tagcttatga
     5041 tccagaaaaa accttgtgtg acatttggaa tccctggtat aacgttgagg atgaaatcaa
     5101 agtaaaagca atcaaaaact acatggagtc ggacagaaaa aatcttagaa aattgaatga
     5161 gtatcgacga attttgccta cagataaaac gatgcgctct tatattatgg ctttaaatta
     5221 aaggagaa
//
LOCUS       DACCCM010000124         5120 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCCM010000124 DACCCM010000000
VERSION     DACCCM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.129"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(207..1169)
                     /locus_tag="IVK78_002685"
     CDS             complement(207..1169)
                     /locus_tag="IVK78_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6376083.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVK78_002686"
     CDS             complement(1171..2610)
                     /locus_tag="IVK78_002686"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP6376084.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVK78_002687"
     CDS             2795..4741
                     /locus_tag="IVK78_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP6376085.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5120
                     /locus_tag="IVK78_002688"
     CDS             5005..>5120
                     /locus_tag="IVK78_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6376086.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         1665 a          855 c          841 g         1759 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat
//
LOCUS       DACCCM010000125         5032 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCCM010000125 DACCCM010000000
VERSION     DACCCM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.130"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(178..681)
                     /locus_tag="IVK78_002689"
     CDS             complement(178..681)
                     /locus_tag="IVK78_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6376087.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(804..1568)
                     /locus_tag="IVK78_002690"
     CDS             complement(804..1568)
                     /locus_tag="IVK78_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP6376088.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1676..1951
                     /locus_tag="IVK78_002691"
     CDS             1676..1951
                     /locus_tag="IVK78_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP6376089.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(2152..2499)
                     /locus_tag="IVK78_002692"
     CDS             complement(2152..2499)
                     /locus_tag="IVK78_002692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376090.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            2667..3596
                     /gene="yidC"
                     /locus_tag="IVK78_002693"
     CDS             2667..3596
                     /gene="yidC"
                     /locus_tag="IVK78_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6376091.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            3699..5032
                     /locus_tag="IVK78_002694"
                     /pseudo
     CDS             3699..5032
                     /locus_tag="IVK78_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1593 a          963 c          943 g         1533 t
ORIGIN      
        1 caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct tgcatctgca
       61 gtaataagcc gaaacttcac aaaaatttga gaagtaattt tcgtaaattc cttcttattt
      121 cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt ttacctgtta
      181 aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg cagacatggg
      241 ctgagccgct tctttgactg cgcagtattt gtccacgaat gtcacaatat atccttcttt
      301 atacttaggc ggtgcgatcg ttgcacccca catgtgcttc aaaatgatat ctcgctcaag
      361 atccgacaaa tcggtgattt tttctgcatt ctttacagca attcttggat ggatatacgc
      421 atgtgtcccc tcgtcaaatt tcgtcgtacg ccaatcatag tagaacagat catgcaaaag
      481 tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca gataactata
      541 gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg tgtaattcgc
      601 taattttttc actgcttctg tttctaaaag atcttgtaca taagacatat attcttcgtc
      661 ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt cattttcttc
      721 tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgctct agataaaata
      781 gctaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacgat ccctgctgcg
      841 actccgacat tcaatgattc cgcatttcct ttcattggga tatatacgtt ctgatcagtc
      901 aaagacaaga tttcctgact gactccctga ccttcgtttc ccatgattat agcgaaatcc
      961 tctgtttttt ctactttgtc taatgcatgt gcttcttcat ttaattctgt accgtacacc
     1021 gtaaaccctt tgcctttcat tacagggatt acatctgcca aatctttttg taacacaggc
     1081 aaatgataat tgcttccctg catgcttcgc agcacctttg tactataaat atctgccgtt
     1141 cctttaccta ataaaacacc agccatacca aacgcatctg ccgtacggat catcgttccc
     1201 acatttccag gatcctgtac attatctagt aaaagccagc ctcctgaaaa atctggcgta
     1261 tcttcagatg ttaacgtcat cactgctaag atcccttgcg gtgtaggtag atctgacata
     1321 gcggtcatga cctcatctga aactagaaaa gtagaaatct ctttgtttac agccagccag
     1381 tctccccatt ctttcatacc tctctgtgtc acaaaaagtt cttttatttc tgcatcagac
     1441 gacgcagctt cttcgatcag atggaaccct tccaaaagat attctgcctt ttcttctctg
     1501 tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc tgattggatc
     1561 tctttcacat tccttcacct cacctgatta tatcatggaa taggtgcttg agcttgtttc
     1621 atcctaaaaa aatcggtatc attgatttat aaaacaggaa agaaaggtga cgaacatgcg
     1681 caaagttaaa atgaatgtcc aaggacgtgt ccagggcgtg ggatttcgct atatgaccaa
     1741 gatggttgct gatcagttag gcgtgaccgg aacggtaaaa aacgaagacg acggctcagt
     1801 cacaatcgaa gcaatcggaa atgacgacat catccaaaaa tttatcgaag aagtcaagaa
     1861 gtctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagatc cgatgattga
     1921 ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaggtcgctt agttttgagt
     1981 attaaggaaa attttgtgaa aatggctttc cacatttttg cataaatttg acttaatacc
     2041 gagaaactga cttttgaaca ccgtcgataa ggttgttttt gaagcgcttt gcctcgagta
     2101 ttaaggagaa acattttcaa aatagcttct catattttga aatgtaatga cttaataccg
     2161 agaggcagct gataaaacac cgtcaataag gttgtgaaaa agctggtctg catcaagcat
     2221 taaggactgg ttgtgaaaaa gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2281 ggctttccac atttttgcat aaatttgtct taataccaag aaactgactt ttgaacaccg
     2341 tcgataaggt tgtttttgag gcgctttgcc ccaagtatta aagacaggta gtaaaaaagc
     2401 tgatctatca cttgcagtga gttcatttga ctcctctatt tcgggcagaa taaaaaaagt
     2461 ttttagtttt ttaaataaga aagaaacact tggaatcact cttttctttc caagtatttc
     2521 tttcttgatt ttttgtcttt tttcaaaatt tttcgctacg aaaaagaaag aatatttata
     2581 tgaaatttct atgaagttga ttgaattcca tgcgcttttt tagtatagtt aagtttgtgt
     2641 aaaaattata gaagaggaag tattctatga ataaatggaa aaactggtta ttgggatccg
     2701 gacttgtcag tatcctgctc tttttatctg gctgtgtgcg gatggattca aacggaaacc
     2761 ctgacacttc aggaatcgtt tatcgagtac tcgtccatcc tatgggccaa gcgattacat
     2821 atctagttga gaatttcaat tggtcttacg gctgggcagt tattgcaatg actgtgatcg
     2881 tccgtatcat catcttgcca ctaggtatca gtcaatcaaa aaaaacaatg atccaatctg
     2941 aaaaaatgca agcgttaaaa ccacaagttg aagcagcaca acaaaaacta aaagctgcaa
     3001 caacacgtga agaacaaatg gcagcacaag cagaaatgca acaagtatat cgcgaaaacg
     3061 gattgagtat gaccggcgga atcggctgtc tgcctctatt gatccaaatg ccgatcttct
     3121 ctgccttgta ttttacggct cgctacacag aaggtatccg tgaatcatct ttttacggca
     3181 tcgatctagg ctcaccaagt atggtgcttg ttgccattgc aggggtcgct tatctgcttc
     3241 aaggatatat ttcaacaatc ggtattcctg aagaacagaa aaagacgatg cgtacgatgc
     3301 tgatcgtctc tcctgctatg atcgttttca tgtcgatcag tgcgccagcc ggtgttacac
     3361 tttactgggt agtgggtggt gtgttcagtt gtctgcaaac attcatcaca aacgttatga
     3421 tgaaaccacg tttgaaagca caagtggcag aagagttgaa acaaaatccg ccaaaacaag
     3481 tcgtcactcc aagaaaagac gtgacagaat cggcaaagcc agctgacaat aaacaaccaa
     3541 aaaacagtac aaacaacaaa ggccgcaacg caggaaagca gcaaaagcgg aaataaaaat
     3601 atatgacaaa aaactgtaga caaataaaaa aatttctttg ttctacagtt tttttgttac
     3661 aatcaaagaa aacgtcgctt ttcgaggagg aatgtataat gatgtccaaa taatcaatgg
     3721 atagtcgaat ggaagaagcc aaaatttcgc tagatgcact tgttcctaaa aatcatttgg
     3781 tccataaaat tgacaaaata ttgttatttg attttattta tccaattgtt gaatcaactt
     3841 actcgaccat tggtcgtcct agcgttgatc ctgtcatctt agtaaaatta gtattcattc
     3901 aatatttatt taggattcgt tcgatgcgtc agtccattaa agaagctgaa acaaacctag
     3961 cttatcgttg gtttcttggg cttagtttaa tcgataaaat tcctcatttt tcaacgtttg
     4021 gtaaaaacta tgtacggcat ttcagagaaa ccgattgttt gaacagatat ttatcattat
     4081 ccttgaacaa gcagttgaag ctagatttat tcatgaagaa attttgtata tggattcaac
     4141 ctatattaaa gcgaatgcga aaaaacatat ttttacaaga gaaatggtac ataaataagc
     4201 cattttatac caaaaacaat tgaaagaaaa aattaatgaa gcaagaattg cagaaggcaa
     4261 acgtcctttt ggttcaatca ttcatcagga ggtggtagaa gggaaaatta gtgtcgcaga
     4321 tccagaaagt ggttattatg taaaaacaga acaagaaaaa cagtttgctt attctgctca
     4381 taccgtttgt gatgaaaatg gttttgtatt agatgtcatg attacgcctg gaaatctaca
     4441 tgacagccga atgctcgtgc cacaaatcga acgtgttaaa tcatgttgtg gtgtagctgc
     4501 tgatgctgcg tacaaaacac cttggaacgc caagtattta atcgatcgaa aactacgacc
     4561 catttttcct tatactcgac ccaaaagaag caaagaacgt tttaaaaaga aagattttta
     4621 ttatgatccc tattataact ggtacctatg cccaaatgat caaacacttc agtttcgcac
     4681 aactactcga gatggttata aaaaatatgt atctaaatct tttatttgtg aaagttgtct
     4741 attattgaaa aattatacag aaagccagaa acaccagaaa gaaatacatc gacatattta
     4801 ggaagactac ttagatgaag cagaacattt acgccatacg gtgtacaatc gcgaaaaata
     4861 taaaaaaaag aaaagaaacg attgaacgcg tttttacaga cgcaaaggaa aagcatggaa
     4921 tgcgctacac caaatatcga ggcttggaaa aaaccaggtg catacgatgc ttgtttttgc
     4981 cgcaatgaat ctgcaaaaat tagccatttg gcagtggggg catatgtctt ta
//
LOCUS       DACCCM010000126         4917 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCCM010000126 DACCCM010000000
VERSION     DACCCM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.131"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            124..2322
                     /locus_tag="IVK78_002695"
     CDS             124..2322
                     /locus_tag="IVK78_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6376092.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(2780..3651)
                     /locus_tag="IVK78_002696"
                     /pseudo
     CDS             complement(2780..3651)
                     /locus_tag="IVK78_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            3951..>4917
                     /locus_tag="IVK78_002697"
     CDS             3951..>4917
                     /locus_tag="IVK78_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6376093.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDG"
BASE COUNT         1719 a          790 c          960 g         1448 t
ORIGIN      
        1 ttacttggat ttttattttt aattatgcta ttcttgaaag cggggaatgc tcatttaatt
       61 agatgagtat caattttaca gaaaaatgaa agccttttat tttaaaagga ggagaggaag
      121 aaaatgaaga aaagtacagt attttataaa atatttttac cgatgttttt attaggagcc
      181 gtacttgtca taggatttag tttattcatt taccagaata cctatgaatc gatcgaatcg
      241 agttatttaa cagacaaaaa gaatttgctc aggcaagtta aaactaacgt tgaatggaaa
      301 attagaacga ttgaatattc gttttctacg tatggttcga ccaaaaattt ctcggacata
      361 ttcaagcatc cgctgacata tacagattac tccacttatt ctgaaatcag gaaagaacta
      421 aattttatag aaacgattgt tatggatgat aataattatg atttagtcag tttggctggt
      481 aaatggggag taatcaatgg atcgttgagt caactgaccg aagaggaaat tgaaaagtat
      541 caacagaagt acataaataa caaaaataat ttattttggc aaaagaaaaa caaaggaatc
      601 gaaatggtga tttctttgcc aatgtttgaa aaagaaaaat atgcattggg aattgctacg
      661 atagagaaac atacaataga acaagttgtt ggtgaaagtg gagaagacat actttctatt
      721 gaaaacgaag ttggcaacct ttttactact cagacagtaa aaaataaaga gcaaccaact
      781 gtattaacgg acgtggatct agattacaat cagctgaaag tgataagaga aaaagataaa
      841 acgtatatag tactgaaatc agattataat aattggattt acagattaga agttgactct
      901 agtgcaattg gcgcaatgat ccgcaatctg agaatcgggc tagttgcagt gtctttaaca
      961 ctggtaggac tcattggtct tctttcctac ttattttcgg acagatttgc ccgtccgatt
     1021 tctcaaatac aggaacgtct gaatcttaaa tcaaaaggat tcagtgggaa agaactagat
     1081 ttggtcgctc aatcagtcag tcggatcata ggagaaaaag aagcgttaag tgcacactta
     1141 gttacccaaa agccacagct ggaaacgcta tttgtactta gtttatttag aaatcgagta
     1201 gaaaggcgag aactagatca gcggttgcag caatttggtt atagttctca aaatgactgc
     1261 tattatacag ctttggttca aatcgatctg ttagaagata gtcacggtgg ggagcgtgat
     1321 ttgctacttt tagcgatcaa caacatgatc acggaaattg tgccaaagga aaatcgaatg
     1381 ctgccgatcg ttttgaatga agaaatgcaa gccactattt atcgaatgga taaagatgat
     1441 gcagaagcaa gcaaaaaagt catggagtat tgccgactca ttcaaaaacg tgtaaaagaa
     1501 tacttgaatt taactatcag tataggcatc agtaaccggt tcgaaacgct gaatgaaagc
     1561 aagcaatcgg ttgatcgggc aaaagaagcc ctgtattatc gtgtaaatac tggtccaagt
     1621 tcaattattt tttatcagga aatcgttcct gctgctcatg aaaaaacact tattagatat
     1681 ccgatagaag aacaaaatcg attgtttgaa gccattcgtt ctggaaatca ggaagtgaag
     1741 aatttagtac atgaattgat tgatgcattg tttgcacaaa ataaaaatcc tttgagtcgg
     1801 gaagtagttt cggtccgctt gatcaatgaa ctcgttcaat taggacagtt attgggagta
     1861 gattcaaaaa actttgataa catgaaacaa atttatgtaa aagcactgaa caattatcat
     1921 cccaaagaac tagaaaaact tgttttagaa caattagttc tgccaatcac agaatgctct
     1981 caagcaatca ctgatcatga atttaaaaat ttgtcggaga atattatgca tatcgtctac
     2041 acagaatatg atcgtgatct gtctttagat gtgatcgctg ataggttgca ttataaccct
     2101 aactatttaa gtaatgtatt taaaaaggaa acaggagaaa aatttggtga ttttgttcaa
     2161 aatcatcgtt tagaggtagc aaaaaaatgg ttgaaagaga ctaacctctc cgtaaaagag
     2221 attgcagaaa gattgcaata tcgaaatccg caaaatttta ttcgtttctt taaaaagaag
     2281 gaatcgatta cgccgggaga atatcgaaaa aaatatttgt gaatcaagag gctgtttttc
     2341 ttgtggactg attcaagcaa taaaagaagt tagaataacc ctagtatttg tatttatgga
     2401 tagttaaatt tcaaggctaa gcacgcaaaa agaactgtga caaaagtctt gtcacagttc
     2461 ttttttacga ataaatggcg ttcaagagac aggctatcga caataaggca taattttgtg
     2521 aaatatggga agctattttt caaaatcgct tcttattgct cctgtgggtt actcgtcgca
     2581 atgtttgtga gagtttttat catcgctcaa ggctgaacgt ctctttcaca acctctgata
     2641 cacggtgttc caggagtaga ttcttcgaaa aataagccga aatttcacaa aaatttaaga
     2701 agcaattttc gtaaatgcct tcttatttct tgaagctgac cacttctgac acaactacca
     2761 ttttgcgttc agtacataat caccctttga cagaacctac gagtgctcct ttgacaaaat
     2821 atttctgaac aaaaggataa acaatcagca taggcactgt tgcaataacg ataacagcca
     2881 ttttaactgt ttgtgctggt ggaatcacat caacggcact actttctgct tgcatcccac
     2941 ttgatacaat gacgatttgg cgtaaaagaa tctgtatcgg ccatttagat gaatcgttga
     3001 tatacaaaat agcagtcata taagtgttcc agtaagtaac agcataaaaa agcgagatag
     3061 tagcaataga cggtaacgct aatggcagca taattttgaa aaatacacca aaatctgtgc
     3121 aaccatccat ttttgctgat tcttccaaac tatccggcaa ggcttggaaa aagtttctca
     3181 taatgatcat attcattgca ttaacagcca caggcaggat catggcccag taagaattga
     3241 ccagccctag agaacgaaca actaagaacg ttggaatcat cccccgctga acaacataga
     3301 aaatacaaca agaaagttga cgaaacctcg accaaacaaa tactttctgg aaagaccgta
     3361 ggccatcagt gaagttgcac acatactgac gatcgttccg acacctgtaa caccaatcga
     3421 aacagcaagt gctctaaaaa tggtcggcgt agaaagaatg tagcgatacg cgtctaaaga
     3481 aaaggtagta ggaaaaagga taaacttttt cgcgacgact tcttgtgtag aagcaaaaga
     3541 tgtagcgatg atatttaaaa aaggaacgat acagatcaaa gctagcaaaa ttagaaacgt
     3601 attgttgaaa agtgtaaaac ttctttctcc tatagagact ttctttttca tatagatagg
     3661 ctcctcctca aaataaatta aatgtttctg tttctatatg ttcaatttat catcggtgaa
     3721 agattacgta tataatcatt tcctatgcta agtgtcagat agaaaaaaat agaaggtttg
     3781 ctatgcgtag tttatttgtg aaaaaataca ctttatcgtt ttgtatagca caaaaatcat
     3841 tgctatgtaa gcgtttttac ttacggtaaa aatgattata tacgtaattc agatagttat
     3901 ctatcatgaa gtcataagca gcaaacattc acaaaactaa ggaggggctt atggaaagtg
     3961 taaagaaaca agaaccattt caactgacgc tatcgaaaaa agaagaaaag ctcagaagac
     4021 gaaaaaaaat aattgcagga atcaaaacga ataaattttt atatgtgatg attctgccgg
     4081 gagtccttta tttcttgatt tttaggtatc ttcctatggg gggactcgtt attgcttttc
     4141 aagattatca accattttta ggaataatgg gaagtccttg ggtcggtttc aaacatttca
     4201 ttcgtttgtt tacagaaccg acattcttta cattattaag aaatacgttg atcttgttcg
     4261 gattaaatat cttcgtgttt tttccgatgc caatcatttt gtctttacta ttaaatgaag
     4321 taacgaacaa acgattgaaa aacgggattc aaacgatcat ttacatccct cacttcatgt
     4381 cttgggtaat cattgtttcg attacttatg tatttttgaa tgttgacgga ggggtattga
     4441 atgaattcct tgcaacaatg ggatttgaaa aaatcagttt tctaacatcc cctgaatgga
     4501 cacgcacagt atatatcgga caagtgatct ggaaagagtt gggctggtct acgatcatct
     4561 acctttcagc gataactgca gtcgatacac agttgtatga agctgcagaa atggatggag
     4621 caggacgctt acgcaaaaca tggcatgtta cattgccggc aattcgtcca gtaatcatta
     4681 ccttgctgat tctaaaaatc ggtcagacat tagacttagg gttcgagcat atgtacttgt
     4741 tattgaattc attgaatcga agcgttgcag aaattttcga cacctatatt tatacagctg
     4801 gattgaagaa tggacagtta agtttcagta cagcaatggg attgtttaaa ggggtcgtag
     4861 gattgtttat ggtaatttta gctaataaga ttgccaaaaa gctaggagaa gacggcg
//
LOCUS       DACCCM010000127         4807 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCCM010000127 DACCCM010000000
VERSION     DACCCM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.132"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVK78_002698"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVK78_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP6376094.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IVK78_002699"
     CDS             complement(3250..3768)
                     /locus_tag="IVK78_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP6376095.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          787 c          730 g         1711 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgccggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACCCM010000128         4805 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCCM010000128 DACCCM010000000
VERSION     DACCCM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.133"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(51..1199)
                     /gene="nagA"
                     /locus_tag="IVK78_002700"
     CDS             complement(51..1199)
                     /gene="nagA"
                     /locus_tag="IVK78_002700"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP6376096.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1275..2111)
                     /locus_tag="IVK78_002701"
     CDS             complement(1275..2111)
                     /locus_tag="IVK78_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6376097.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2295..3512)
                     /locus_tag="IVK78_002702"
     CDS             complement(2295..3512)
                     /locus_tag="IVK78_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6376098.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3895..3978
                     /locus_tag="IVK78_002703"
     tRNA            3895..3978
                     /locus_tag="IVK78_002703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3929..3931,aa:Leu,seq:gag)
     gene            complement(4218..>4805)
                     /locus_tag="IVK78_002704"
     CDS             complement(4218..>4805)
                     /locus_tag="IVK78_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376099.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1458 a         1001 c          776 g         1570 t
ORIGIN      
        1 ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg
       61 aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata
      121 aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt
      181 gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca
      241 acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga
      301 cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc
      361 atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc
      421 attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag
      481 acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc
      541 ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct
      601 tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg
      661 acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct
      721 gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt
      781 tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta
      841 gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct
      901 ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca
      961 gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga
     1021 atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact
     1081 tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag
     1141 cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt
     1201 ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga
     1261 atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca
     1321 tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat
     1381 aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc
     1441 tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa
     1501 atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt
     1561 cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata
     1621 caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag
     1681 cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg
     1741 caaacaccga gataataata atagcgttga aagtcagttg cttgatacag attttcagaa
     1801 atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta
     1861 tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca
     1921 tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt
     1981 tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt
     2041 ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt
     2101 aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact
     2161 ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta
     2221 agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa
     2281 tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct
     2341 gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc
     2401 caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg
     2461 aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa
     2521 gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat
     2581 tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg
     2641 cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac
     2701 aacatattca gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc
     2761 cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg
     2821 aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga
     2881 tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct
     2941 tctaataatt cttttggata aagtgcccca gtaggattat taggattaat gataacaata
     3001 gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc
     3061 tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac
     3121 aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata
     3181 cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc
     3241 ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg
     3301 gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt
     3361 ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc
     3421 tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct
     3481 tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag
     3541 ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa
     3601 ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac
     3661 ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcga ttccacaaat
     3721 gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa
     3781 gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat
     3841 ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat
     3901 gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt
     3961 cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt
     4021 ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac
     4081 aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac
     4141 ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc
     4201 gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc
     4261 acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct
     4321 ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt
     4381 ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc
     4441 atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca
     4501 attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa
     4561 agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc
     4621 attgcccttt tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa
     4681 ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat
     4741 ttcctatatg ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct
     4801 aaaaa
//
LOCUS       DACCCM010000129         4662 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCCM010000129 DACCCM010000000
VERSION     DACCCM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.134"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(172..474)
                     /locus_tag="IVK78_002705"
     CDS             complement(172..474)
                     /locus_tag="IVK78_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376100.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="IVK78_002706"
     tmRNA           complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="IVK78_002706"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1231..2004)
                     /locus_tag="IVK78_002707"
     CDS             complement(1231..2004)
                     /locus_tag="IVK78_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6376101.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2148..2498
                     /locus_tag="IVK78_002708"
     CDS             2148..2498
                     /locus_tag="IVK78_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6376102.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2479..3195
                     /locus_tag="IVK78_002709"
     CDS             2479..3195
                     /locus_tag="IVK78_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6376103.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3195..4643
                     /locus_tag="IVK78_002710"
     CDS             3195..4643
                     /locus_tag="IVK78_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6376104.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
BASE COUNT         1550 a          822 c          900 g         1390 t
ORIGIN      
        1 ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat
       61 aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat atccctttta
      121 taagtatggt gataaaatcc gttccgggct acctttttta ggtagcctac tttaatcttt
      181 atacctttct ggatcaacga aagtatactt caaatagtca taacgccgat gatcgctccg
      241 tgcgtctggc acatcagtca cgatatcaaa caaaaaatac acatctttct tcattctggt
      301 ttttgttgcc ggaattttaa agtagttctt attagagtaa tagagattga ttaataagct
      361 atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat ctttgacaaa
      421 tctattcgaa ggatcaaatt taaaccataa ttgtgttttt ccttccatca acataatgtt
      481 cacctcaaaa agagtatacg aactaatgtt ctttttgtaa agtaggaata tcattgataa
      541 tatggatgga acatatatat taaaaagtgt tctttttgaa tgctattttg aatgccattt
      601 tcattcattt aacgaaaatc tgcgaaaata aaaaatgctc aaacccttat aaaataagga
      661 tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga gtcgaacccg
      721 cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac
      781 tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac
      841 ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg
      901 gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt
      961 cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca
     1021 attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg
     1081 tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa
     1141 tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct
     1201 taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg
     1261 gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc
     1321 ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg
     1381 accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt
     1441 gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg
     1501 ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat
     1561 cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga
     1621 atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg
     1681 atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca
     1741 ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc
     1801 attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc
     1861 tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt
     1921 tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa
     1981 agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct
     2041 taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag
     2101 tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca
     2161 gtattggtat cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa
     2221 atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc
     2281 tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg
     2341 gctattattt aaagacatct gggcatacga attattttct gattgaaatg cttctgctag
     2401 tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg
     2461 aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa
     2521 atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa
     2581 gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac actagacctg
     2641 aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact
     2701 gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag
     2761 atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg
     2821 aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt agtttcagat
     2881 gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat
     2941 ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta
     3001 gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat
     3061 cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc
     3121 gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat
     3181 tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc ggaatggtcg
     3241 cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag
     3301 aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct
     3361 atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga
     3421 cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg
     3481 tcatttatcc gactaataca gctttactga attttacctt aacagctgat caatggaaaa
     3541 atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac
     3601 aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg
     3661 tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt
     3721 tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat
     3781 tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt
     3841 ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca
     3901 ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc
     3961 gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg
     4021 gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta
     4081 aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg
     4141 aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat
     4201 tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat
     4261 tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg
     4321 tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag
     4381 aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac
     4441 aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa
     4501 aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag
     4561 gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg
     4621 acgtagaaat caaaaaagag taaaaaacaa gcggtataaa ag
//
LOCUS       DACCCM010000130         4616 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCCM010000130 DACCCM010000000
VERSION     DACCCM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.135"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(15..962)
                     /locus_tag="IVK78_002711"
     CDS             complement(15..962)
                     /locus_tag="IVK78_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6376105.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IVK78_002712"
     CDS             complement(1140..1943)
                     /locus_tag="IVK78_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP6376106.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="IVK78_002713"
     CDS             2089..3003
                     /locus_tag="IVK78_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6376107.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="IVK78_002714"
     CDS             3051..3521
                     /locus_tag="IVK78_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP6376108.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="IVK78_002715"
     CDS             3606..4214
                     /locus_tag="IVK78_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP6376109.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="IVK78_002716"
     CDS             4348..4548
                     /locus_tag="IVK78_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6376110.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttag
//
LOCUS       DACCCM010000131         4533 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCCM010000131 DACCCM010000000
VERSION     DACCCM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.137"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            544..1107
                     /locus_tag="IVK78_002717"
     CDS             544..1107
                     /locus_tag="IVK78_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6376111.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IVK78_002718"
     CDS             complement(1386..1673)
                     /locus_tag="IVK78_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6376112.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IVK78_002719"
     CDS             complement(1663..1992)
                     /locus_tag="IVK78_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6376113.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IVK78_002720"
     CDS             2297..2626
                     /locus_tag="IVK78_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6376114.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVK78_002721"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVK78_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6376115.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4468)
                     /gene="vanY"
                     /locus_tag="IVK78_002722"
     CDS             complement(3551..4468)
                     /gene="vanY"
                     /locus_tag="IVK78_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP6376116.1"
                     /translation="MKKLFFLLLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKE
                     HLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYG
                     LLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPA
                     GYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWH
                     IRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPT
                     NLRYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1503 a          762 c          672 g         1596 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataacaataa aaaaaacaac ttcttcattt tcagtctcct ccttaactat atagttggaa
     4501 gtatgaaata tttctagaat cttcccctta aac
//
LOCUS       DACCCM010000132         4477 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCCM010000132 DACCCM010000000
VERSION     DACCCM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.138"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(832..1125)
                     /locus_tag="IVK78_002723"
     CDS             complement(832..1125)
                     /locus_tag="IVK78_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP6376117.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1528..2706)
                     /locus_tag="IVK78_002724"
     CDS             complement(1528..2706)
                     /locus_tag="IVK78_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6376118.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="IVK78_002725"
     CDS             complement(2810..3124)
                     /locus_tag="IVK78_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6376119.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="IVK78_002726"
     CDS             3231..3446
                     /locus_tag="IVK78_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376120.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="IVK78_002727"
     CDS             complement(3746..3988)
                     /locus_tag="IVK78_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6376121.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="IVK78_002728"
     CDS             complement(3975..4403)
                     /locus_tag="IVK78_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6376122.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFLI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1417 a          841 c          664 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaggaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DACCCM010000133         4448 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCCM010000133 DACCCM010000000
VERSION     DACCCM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.140"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            431..1045
                     /locus_tag="IVK78_002729"
     CDS             431..1045
                     /locus_tag="IVK78_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6376123.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1210..2214)
                     /locus_tag="IVK78_002730"
     CDS             complement(1210..2214)
                     /locus_tag="IVK78_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376124.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(2673..2930)
                     /locus_tag="IVK78_002731"
     CDS             complement(2673..2930)
                     /locus_tag="IVK78_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6376125.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2923..3192)
                     /locus_tag="IVK78_002732"
     CDS             complement(2923..3192)
                     /locus_tag="IVK78_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6376126.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(3323..3502)
                     /locus_tag="IVK78_002733"
     CDS             complement(3323..3502)
                     /locus_tag="IVK78_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376127.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(3883..>4448)
                     /locus_tag="IVK78_002734"
     CDS             complement(3883..>4448)
                     /locus_tag="IVK78_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376128.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1412 a          814 c          627 g         1595 t
ORIGIN      
        1 cacattatac aaacatatgt tcgatattgt aaagaagaaa tagctaaata cataaaaaaa
       61 gaagcatagg tatgctatca ccctgcttct tcttttgatt tgtatgatca atttattcta
      121 tcactttact aattaatttg aaaatctaat aaaaactctc actctaaaag agtgagagac
      181 tgcaaattag agtacggact tttttggagt tccgttcttt aatattaaca aatgaataaa
      241 atccttacaa catttgagaa taagttatca gtagacactt acaaaaaatt ctcatactat
      301 ttcctccatc attagttttt gtctatataa ggtttaccct aaatagacac tggtataaca
      361 ttgaaaaata tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta
      421 ggtaaaagtt ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg
      481 ccagattgaa gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg
      541 cgcaacgatt actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga
      601 tatttttatt gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc
      661 agtcaattac ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc
      721 tgaagcaatt ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt
      781 ggccatgatc gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa
      841 gatcgcaaaa gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa
      901 agatccccaa cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc
      961 gattgctaag attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa
     1021 agacagcatg gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta
     1081 atttcctaca tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata
     1141 aaattccaac aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac
     1201 ttatttacgt taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc
     1261 cttattatct tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact
     1321 aattcctata gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat
     1381 ttgagattta tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata
     1441 cataaagcta tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca
     1501 ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg
     1561 tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta
     1621 ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat
     1681 tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt
     1741 aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat
     1801 aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga
     1861 agttatatct ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat
     1921 ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc
     1981 aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc
     2041 aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc
     2101 aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat
     2161 tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc
     2221 tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta
     2281 atttttagct aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact
     2341 agtacaaaaa atttaaaata gattattaaa cctattgctt attctattca gtcatatata
     2401 taagcgtcat cctttagtaa ctaacatccc acactacaat agtattataa catatctatt
     2461 caacttcaaa ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg
     2521 attttactaa ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac
     2581 actaagctat attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt
     2641 aagtttctga ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata
     2701 aatatcgttt cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac
     2761 cattttccag ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa
     2821 cgatcgatat cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca
     2881 tgccaataaa gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac
     2941 ctcgattaag tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat
     3001 tttttccatg acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc
     3061 tctttttgat aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc
     3121 agcatcctca ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc
     3181 tactgcttcc attcctttca ccccttattt atttctataa aacaattgta cattatattg
     3241 tacaattaag caaatgatta atattctaac ttcttataat taaaagtgct atcactttta
     3301 gtttttagga taaaaagtga cgtcactttt tgagtgctgg tacctcaaat cctgcttttt
     3361 cttgggcaat caataaatct aacgcaatac acataatcct ggattcactt ttgccgtact
     3421 tctgctttag ttgctgcaat tctttttgtc tcttttcatc caacgtaacg ggtaaccgtt
     3481 tatttcctat tgcaattggc attagtactc tctcctttca tcgaaataac cctatgaaaa
     3541 atatggctgc aatcatcttg aatagtagac tcacaagggc taatacaaag cgccaaaaga
     3601 tcaaacaaaa taccagtatt gccaaaaata ctacgaagtt taaatgcaca gctgcagcat
     3661 ggcgcataag tatttcgcca acaaatagga ctaacaatag aggaatgatt ttattcatat
     3721 ggatctcctt ctttctctta ggttatcggg tgttgcccgc ttatttcgca ccacctaagc
     3781 ggcgggggcg gttggagcga ttggtgcgaa ataagaacca ttcccttagt tactaggttt
     3841 tttgtcctta tttcacacat tctcactttt cggtataaat gattattgcc tttttttcct
     3901 ttcaaatcgt tgatatggca ttcgtttacg gtcaagttca ttttggattt cttctgtatc
     3961 gtcgtaagta taggtgtcta aaaaatcagt taaagtcgtg taccagtcgt cgcctgggtt
     4021 atctacttct tctacatatt ccctcaaaaa tgcttcgggt acagcgagtt cataatccat
     4081 tcccattaac acgatagatt ccgttccaca cgtcaaaaat ctagtaaata caaggtaata
     4141 atcgtctatc gtcgcatctt tgctgtttag ctcttccatt tcttcttcca ttaattccat
     4201 gctctcaaaa attgaataat attctgattc tgagtaatag tgtttcctgc aaatcgtgta
     4261 gctgacaaag ttttcgacca gctcactcct ttctaggtaa acattcatga tgtatttttc
     4321 tgtttctagt cgtagaagct ccgttccttt ttttaccgtt tcaggacgac gtttatacac
     4381 gtctaaacga ttatggctga ctgtgacctt cgtttgactc gtttcacttt ccaaatacaa
     4441 ttgttctt
//
LOCUS       DACCCM010000134         4430 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCCM010000134 DACCCM010000000
VERSION     DACCCM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.141"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(543..2048)
                     /locus_tag="IVK78_002735"
     CDS             complement(543..2048)
                     /locus_tag="IVK78_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6376129.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVK78_002736"
     CDS             complement(2045..2731)
                     /locus_tag="IVK78_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6376130.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVK78_002737"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVK78_002737"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP6376131.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DACCCM010000135         4401 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCCM010000135 DACCCM010000000
VERSION     DACCCM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.142"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(99..1619)
                     /locus_tag="IVK78_002738"
     CDS             complement(99..1619)
                     /locus_tag="IVK78_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6376132.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1715..1840)
                     /locus_tag="IVK78_002739"
     CDS             complement(1715..1840)
                     /locus_tag="IVK78_002739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6376133.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(1862..2047)
                     /locus_tag="IVK78_002740"
     CDS             complement(1862..2047)
                     /locus_tag="IVK78_002740"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP6376134.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="IVK78_002741"
     CDS             complement(2031..2213)
                     /locus_tag="IVK78_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376135.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="IVK78_002742"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="IVK78_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="IVK78_002743"
     CDS             3167..3766
                     /locus_tag="IVK78_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6376136.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4401
                     /locus_tag="IVK78_002744"
     CDS             3830..>4401
                     /locus_tag="IVK78_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376137.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         1403 a          799 c          785 g         1414 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag a
//
LOCUS       DACCCM010000136         4382 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCCM010000136 DACCCM010000000
VERSION     DACCCM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.143"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(306..836)
                     /locus_tag="IVK78_002745"
     CDS             complement(306..836)
                     /locus_tag="IVK78_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6376138.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1018..1674)
                     /locus_tag="IVK78_002746"
     CDS             complement(1018..1674)
                     /locus_tag="IVK78_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6376139.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1801..2274)
                     /gene="dfrF"
                     /locus_tag="IVK78_002747"
     CDS             complement(1801..2274)
                     /gene="dfrF"
                     /locus_tag="IVK78_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6376140.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2693..2911)
                     /locus_tag="IVK78_002748"
                     /pseudo
     CDS             complement(2693..2911)
                     /locus_tag="IVK78_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2942..4156)
                     /locus_tag="IVK78_002749"
     CDS             complement(2942..4156)
                     /locus_tag="IVK78_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6376141.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1340 a          932 c          645 g         1465 t
ORIGIN      
        1 gaaatcaact gtctttctca tatttactat tcaattttca tagtgatata ggtacaattt
       61 atatcactac tgtaaaaatg gggttttgcc ttataaatac agcgttttcc gcttgttagt
      121 gtatagtgtt tcttgttttt tgtgcccccc ttgttagata acgggggctt tgaacggctc
      181 gcaagctcgc cgaaccgccg aagcgaaccg cccccttgca agcatggcgg aaaaatctta
      241 aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt tctgtttctc
      301 tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg tgttttacaa
      361 agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt tcctaacaga
      421 gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa ctgctgaaca
      481 agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag ccgttccaat
      541 aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca ctctttatgt
      601 gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag ttcagtaaca
      661 acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt aaattctgta
      721 taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac tccaccatta
      781 ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt tctcattttt
      841 tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag ggaacaactg
      901 attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa atcactaacc
      961 atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa tttttgccta
     1021 tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc aatgtcttga
     1081 aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa tcagccaaat
     1141 tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc tttctttcgg
     1201 tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag tgctttccca
     1261 tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa agtctgcaat
     1321 ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac gctcttgata
     1381 attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa ctatatcttc
     1441 aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac catcagcgtt
     1501 tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag atactaaggt
     1561 ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat aagtatcttt
     1621 cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat tcatattcaa
     1681 tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct tacaacaatt
     1741 tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa tttactcaat
     1801 tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa gtgtttcccc
     1861 tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc catcttcaat
     1921 atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt ccttaaatag
     1981 tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg catcttctaa
     2041 tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa caacaatatt
     2101 cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca taataaccac
     2161 attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc atggtatatt
     2221 accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta tcatctattt
     2281 catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca tcttaacaaa
     2341 ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc atctgcatta
     2401 tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta cttatcagcc
     2461 tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg acaaaccata
     2521 gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt cacggcaaca
     2581 aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat attcttttgt
     2641 ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa acaccttttt
     2701 cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca
     2761 aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt
     2821 catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt
     2881 ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt
     2941 ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg
     3001 tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg
     3061 catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg
     3121 gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc
     3181 cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa
     3241 aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg
     3301 cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta
     3361 cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg
     3421 caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa
     3481 gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag
     3541 ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg
     3601 tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga
     3661 aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga
     3721 aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc
     3781 ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa
     3841 agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc
     3901 tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca
     3961 atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag
     4021 acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag
     4081 gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga
     4141 taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt
     4201 agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt
     4261 aataaaagct tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata
     4321 aacggtcttc tttttataga aaaccgttta ttttcgtctt tttttatttt taaagatatt
     4381 tt
//
LOCUS       DACCCM010000137         4201 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCCM010000137 DACCCM010000000
VERSION     DACCCM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.144"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(3..1223)
                     /locus_tag="IVK78_002750"
     CDS             complement(3..1223)
                     /locus_tag="IVK78_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6376142.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IVK78_002751"
     CDS             complement(1243..1848)
                     /locus_tag="IVK78_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6376143.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IVK78_002752"
     CDS             complement(1897..2829)
                     /locus_tag="IVK78_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6376144.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="IVK78_002753"
     CDS             complement(3254..4111)
                     /locus_tag="IVK78_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6376145.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1192 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
     4201 a
//
LOCUS       DACCCM010000138         4175 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCCM010000138 DACCCM010000000
VERSION     DACCCM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.145"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            612..1424
                     /locus_tag="IVK78_002754"
     CDS             612..1424
                     /locus_tag="IVK78_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376146.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="IVK78_002755"
     CDS             3059..3613
                     /locus_tag="IVK78_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6376147.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4175
                     /locus_tag="IVK78_002756"
     CDS             3867..>4175
                     /locus_tag="IVK78_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376148.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         1542 a          516 c          661 g         1456 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gtttg
//
LOCUS       DACCCM010000139         4156 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCCM010000139 DACCCM010000000
VERSION     DACCCM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.146"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            178..774
                     /locus_tag="IVK78_002757"
     CDS             178..774
                     /locus_tag="IVK78_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6376149.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IVK78_002758"
     CDS             898..2697
                     /locus_tag="IVK78_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6376150.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IVK78_002759"
     CDS             complement(2975..3187)
                     /locus_tag="IVK78_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376151.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4051..>4156)
                     /locus_tag="IVK78_002760"
     CDS             complement(4051..>4156)
                     /locus_tag="IVK78_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6376152.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1492 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcg
//
LOCUS       DACCCM010000140         4112 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCCM010000140 DACCCM010000000
VERSION     DACCCM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.148"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(39..281)
                     /locus_tag="IVK78_002761"
     CDS             complement(39..281)
                     /locus_tag="IVK78_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6376153.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(313..870)
                     /locus_tag="IVK78_002762"
     CDS             complement(313..870)
                     /locus_tag="IVK78_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6376154.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(883..1071)
                     /locus_tag="IVK78_002763"
     CDS             complement(883..1071)
                     /locus_tag="IVK78_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6376155.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="IVK78_002764"
     CDS             complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="IVK78_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6376156.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2880..3353
                     /locus_tag="IVK78_002765"
     CDS             2880..3353
                     /locus_tag="IVK78_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6376157.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3362..3589
                     /locus_tag="IVK78_002766"
     CDS             3362..3589
                     /locus_tag="IVK78_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6376158.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1234 a          740 c          617 g         1521 t
ORIGIN      
        1 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
       61 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
      121 attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca
      181 atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct
      241 ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg
      301 acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt
      361 gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc
      421 tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt
      481 gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc
      541 agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac
      601 gatcacatct agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt
      661 tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt
      721 caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc
      781 ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc
      841 atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg
      901 atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat
      961 tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc
     1021 aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc
     1081 ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct
     1141 gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc
     1201 gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc
     1261 gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat
     1321 gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt
     1381 tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac
     1441 gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg
     1501 ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat
     1561 atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat
     1621 aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc
     1681 tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta
     1741 aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg
     1801 aaataacaat tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat
     1861 attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta
     1921 tttattaaat tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc
     1981 gttcttactt tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc
     2041 ttataaataa aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa
     2101 cgattggcta tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat
     2161 tcagttggac tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac
     2221 aaaattatcg aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta
     2281 tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac
     2341 ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga
     2401 tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata
     2461 aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa
     2521 taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct
     2581 catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta
     2641 ttgtccaata taatcccagg catctaaact agcattatag aagagacaca catagaatct
     2701 aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac
     2761 cttcatactc tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag
     2821 ttgttttaca ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa
     2881 tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt
     2941 tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt
     3001 ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg
     3061 cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa
     3121 gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg
     3181 gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg
     3241 taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa
     3301 aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga
     3361 catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga
     3421 attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc
     3481 aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg
     3541 tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga
     3601 acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt
     3661 acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat
     3721 gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca
     3781 actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca
     3841 ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt
     3901 tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat
     3961 tcttttttag taagctccca tatatactgt aaagccttct atatattatg tcatgttgct
     4021 ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct
     4081 tttttatttt taacattatt ttgataataa tg
//
LOCUS       DACCCM010000141         4068 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCCM010000141 DACCCM010000000
VERSION     DACCCM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.149"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            70..153
                     /locus_tag="IVK78_002767"
     CDS             70..153
                     /locus_tag="IVK78_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6376159.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="IVK78_002768"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="IVK78_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6376160.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="IVK78_002769"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="IVK78_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1608..2273
                     /locus_tag="IVK78_002770"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="IVK78_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="IVK78_002771"
     CDS             2309..2554
                     /locus_tag="IVK78_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6376161.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="IVK78_002772"
     CDS             2657..3526
                     /locus_tag="IVK78_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6376162.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..>4068
                     /locus_tag="IVK78_002773"
     CDS             3507..>4068
                     /locus_tag="IVK78_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6376163.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKV"
BASE COUNT         1449 a          599 c          786 g         1234 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggtta
//
LOCUS       DACCCM010000142         3956 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCCM010000142 DACCCM010000000
VERSION     DACCCM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.150"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..156
                     /locus_tag="IVK78_002774"
     CDS             <1..156
                     /locus_tag="IVK78_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP6376164.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IVK78_002775"
     CDS             175..1452
                     /locus_tag="IVK78_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6376165.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IVK78_002776"
     CDS             1462..2118
                     /locus_tag="IVK78_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6376166.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IVK78_002777"
     CDS             2118..3494
                     /locus_tag="IVK78_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6376167.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IVK78_002778"
     CDS             3624..>3956
                     /locus_tag="IVK78_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6376168.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACCCM010000143         3735 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCCM010000143 DACCCM010000000
VERSION     DACCCM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.151"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..478
                     /locus_tag="IVK78_002779"
     CDS             <1..478
                     /locus_tag="IVK78_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6376169.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="IVK78_002780"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="IVK78_002780"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP6376170.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="IVK78_002781"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="IVK78_002781"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP6376171.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..3697
                     /locus_tag="IVK78_002782"
     CDS             3200..3697
                     /locus_tag="IVK78_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP6376172.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1230 a          596 c          807 g         1102 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
     3721 aagagaaatc aagaa
//
LOCUS       DACCCM010000144         3688 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCCM010000144 DACCCM010000000
VERSION     DACCCM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.152"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..89
                     /locus_tag="IVK78_002783"
     CDS             <1..89
                     /locus_tag="IVK78_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6376173.1"
                     /translation="LYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(705..1745)
                     /locus_tag="IVK78_002784"
     CDS             complement(705..1745)
                     /locus_tag="IVK78_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6376174.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2094..2420)
                     /locus_tag="IVK78_002785"
     CDS             complement(2094..2420)
                     /locus_tag="IVK78_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376175.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2407..3195)
                     /locus_tag="IVK78_002786"
     CDS             complement(2407..3195)
                     /locus_tag="IVK78_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6376176.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1132 a          609 c          514 g         1433 t
ORIGIN      
        1 gattatataa gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa
       61 tcaaggtatt attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattcttt
      121 atttaaaact ttgcaacaga accgtccgtt gcgggtaata ccgtaacact gaagggcacg
      181 cattaaggcg tcatcttaat gctttcctgt aggacactga cggagattac cagtctcccg
      241 agccttacag gggcctttct tttttttgta ttttcaatta tcacaattta tcgtccaaaa
      301 gtgcagaacg aaaatcaatt catcacaagc atatcagaca attaaattta aaacaactaa
      361 aaatatacaa aaaagtgtaa ttgaatatta gcagcactgc ctgaaaagaa gtaatatcag
      421 gcttttcagc ttggggaaat aagtgaatta cctctcttca tgaattatta taactcaaaa
      481 aagcacatat gcaaacacta gttccctatt tttttgaatg aaaaataact aaaatatcta
      541 acgattatga ctttaaaacc tacgtttgtg attgttttca taaactttta atctaaaaac
      601 tccggaataa tagctattta tttgtaacat ttgtaataaa attaaaatca taaaaaagtt
      661 ctaatttaaa attgatcatt tttatagtga tcgcttttta tcaactactc tagccaattt
      721 tccgtactaa ctgttggtaa ttcgttaaat gtttctcttt gttcgtttgc aagctttgcc
      781 gcaatagctt tttcgacaaa ccaatttttt acataaacga aaattagatt ctgcaaattg
      841 tttacttttt cagttttttg tttttgataa gctttcagca aagtcatata taatcctttg
      901 tcagcatcaa ttccataaga ttccagttct tcataaacaa caactgtttg ctctaattct
      961 tcagcttttt tctttgcatt atgaatagtt tggacggttt tttgagcgtc ttcgtaagta
     1021 ctagaaaatg tttttatcaa gtttaataca ttttctggta caaatgtatt tatactgtcc
     1081 tctttcatga tgtcgacgaa gttatctagt aaaacttgat tttgtaactg atcagtttca
     1141 gtgtctatta tgtgtctatt agtgtctata gaattagaat agaatagatt gtctgcgatt
     1201 ttcgcaatta cggttgagtc cttgatatga ctggattttg ccgtatttgc gattttcgca
     1261 attacggtgg tatcattgat ttctcgcggt tttgccgtat ttgcgatttt cgcaattacg
     1321 gtggtatcag tattgtcttc ctcttccatg attttaaaga cgtcttgatt cgttacttct
     1381 ggcttgccta agtacaataa attaggcatg gattcaatct taccgtcacg tttatttact
     1441 cttcctctct tctgtttcag aagacctgct ttttctaatt cctttttgat tttagatact
     1501 ttaccttcgc ggcattgtaa caatttcatt aattcttcaa ctgtgaatat gaaaaatata
     1561 ttatcatcag aatctaccca gttatttttg actgaataat caaatcgatc tttaagtagc
     1621 atataagcaa tttttgcatc atttgataga tccatgtatt tttcgttagt aaaaaatacc
     1681 ttaggcattt gataatattt ttcacgataa atttcttttg atttgtaata attaaaatca
     1741 ttcatagcca atcctctttc tgtaggatag cgttttgttc aaaactatgt tacaatgaat
     1801 taaacaaata tatctatcca ctatatattt ttctactgct gataagtcgc caaactttgt
     1861 taaagcagta gtcttttttt atcttttttt agtttacctt aaaaaataaa ttaaagcaaa
     1921 ccgctctatt tcaaggttta agctcatagt aactatatca cgatattaca ataatattat
     1981 atcacgatat cgtaatatta caatatgaag ataagcgact tatcagcttt gatactactg
     2041 atttttataa aaaaaatatc acgatattat gatatcgtga tattgaatgt aaattaaatt
     2101 ttattttgtt tctttgctag tttatatcct tcaatcaagt attcttcagg tatggacttt
     2161 aatagcgcat cttctataac gtctggttta cttttgtctg attcagcaat agtcgtccaa
     2221 tatttgagtg ctgacattag aattggattt atacgaatgc tatctgctgt tcttacacta
     2281 cttccttgat cttttatgac tttacttata tttttcttgg ttaattcgat ttcttctact
     2341 tcttttgatt taaaattctt tttattttta tttcctagta atctacttac tgcagcttga
     2401 ctgcgattat cttctgccat caaaatcacc aacttctttt aaatattctt ctgtaaatgc
     2461 tttatagtca tcttcagctg ttgagggttt attaaaattg attagatctg atccagcagc
     2521 ttgcgcttcg tcaacggtta ttgtcctacg aatataagtt ttgaaaattt ttgcaccaat
     2581 gactctagaa atttcggttg ctgcagacgt catttcttta gaaatgttat aacgcttatt
     2641 atacttagta aatagaactc ctcgtacttc aagcttgtcg ttcgtatatt cttttaccgt
     2701 ttcaatagtt tcacttaact ttattagtcc ttttaatgag aaatacgcag cttcaactgg
     2761 aatgataatt tcatcagaaa cagtaaatgc gttgactgta agcattccta aacttggtgg
     2821 acaatcaatt ataatataat catagttgtc cttgattggt tgaagtactc ttttaagcct
     2881 gtgttcactt cctacttgag taaattccct ctctgcagaa gaaagggcta tgtcggctgg
     2941 caaaacatca attcctttat agtccagaat agcatctgta atgtcagcct ccccttttaa
     3001 tacttcataa attgtaggtt ggtttgttcc atctatatcg aaacctacat tatctgtagc
     3061 atttccctga ggatcaatgt ccactaaaag tacattttta ccgtaaacaa atttcaaaca
     3121 tgctgctaaa ttaacgctag tcgtagtttt agaaactcca cctttttgat tggctactga
     3181 caatattctt gccattaaaa tatcccccat tcatcttata ttataatata attatatcat
     3241 aatattgtga tattgcaata tcgtatatgc attatattgt aatattgtgt gattattttg
     3301 ttttaaagat atatatcggt tcggatataa agattattca tatgttattc gaaattaaaa
     3361 taatcacttt ttattacttt aaaatttttt attaatgata agaaatatgt tttgcgagca
     3421 gcaactagaa taatttaaac attactaaac tgtctatatt gttactaata attcccttct
     3481 tatagtgcat tttcttagaa aaaaacaata ttacgatatc atgatattaa gatatcgtaa
     3541 tattatacaa ttgtgattga tattatatat gtttttttat ttttttatca tgtttaatct
     3601 tgaaggaatc atattctttt tctaaagaca ggagcttttc ataaacatca gacatgttta
     3661 aactagaggc caggcttaaa tcataaac
//
LOCUS       DACCCM010000145         3681 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCCM010000145 DACCCM010000000
VERSION     DACCCM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.153"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..277
                     /locus_tag="IVK78_002787"
     CDS             <1..277
                     /locus_tag="IVK78_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP6376177.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IVK78_002788"
     CDS             420..641
                     /locus_tag="IVK78_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6376178.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IVK78_002789"
     CDS             657..1499
                     /locus_tag="IVK78_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376179.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IVK78_002790"
     CDS             1562..3364
                     /locus_tag="IVK78_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP6376180.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(3423..>3681)
                     /locus_tag="IVK78_002791"
     CDS             complement(3423..>3681)
                     /locus_tag="IVK78_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6376181.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSLLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1312 a          523 c          749 g         1097 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aacaaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaatggata tagtaatgcc c
//
LOCUS       DACCCM010000146         3674 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCCM010000146 DACCCM010000000
VERSION     DACCCM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.154"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            155..2344
                     /locus_tag="IVK78_002792"
     CDS             155..2344
                     /locus_tag="IVK78_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6376182.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..>3674
                     /locus_tag="IVK78_002793"
     CDS             2341..>3674
                     /locus_tag="IVK78_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6376183.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDD"
BASE COUNT         1397 a          487 c          612 g         1178 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tattttaatt atattaaaaa atgaatttgg aatagaagtt
     3601 aaagattatt ttattgactt agatttatta cagctaataa acagtgtgaa aagaatgtat
     3661 agtggagacg acaa
//
LOCUS       DACCCM010000147         3579 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCCM010000147 DACCCM010000000
VERSION     DACCCM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.155"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            186..1046
                     /locus_tag="IVK78_002794"
     CDS             186..1046
                     /locus_tag="IVK78_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAP6376184.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            1058..1885
                     /gene="panB"
                     /locus_tag="IVK78_002795"
     CDS             1058..1885
                     /gene="panB"
                     /locus_tag="IVK78_002795"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP6376185.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1900..2748
                     /locus_tag="IVK78_002796"
     CDS             1900..2748
                     /locus_tag="IVK78_002796"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP6376186.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2758..3153
                     /locus_tag="IVK78_002797"
     CDS             2758..3153
                     /locus_tag="IVK78_002797"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP6376187.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCEFNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3355..>3579
                     /locus_tag="IVK78_002798"
     CDS             3355..>3579
                     /locus_tag="IVK78_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6376188.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKKRDNLTPEQAELIALRKKNK"
BASE COUNT         1387 a          400 c          703 g         1089 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gatattatga taaattaact atggatgata tttgaaaaaa acatactgta
      121 tatttgattt aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact
      181 tctttatgga ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt
      241 cattcgggaa gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg
      301 aagactcgtc attagcagct tcgaatttta catctagtaa acaatattta aacttaagag
      361 aattagtaga tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg
      421 tatggcaaga aattaaaaat tatcaaatca aaaacaaatt aatctgccat tgcagtggtg
      481 caatatcttc agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc
      541 atcctatgtt ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta
      601 taacaattga aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag
      661 gtaatagtac tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag
      721 tatctagtaa ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg
      781 gatttactga aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa
      841 atataaaaga aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt
      901 caacaattaa aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat
      961 tatcaaaaaa tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat
     1021 tagaaaagta tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt
     1081 taaagaatct aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac
     1141 agcaaaaata attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat
     1201 ggtatgtcta ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac
     1261 tagagcagtt acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc
     1321 ttatcaaact tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg
     1381 agctcaggtt gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt
     1441 aaaggcttct atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt
     1501 tggaggattt aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc
     1561 taaagctgta gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct
     1621 tgcagagtta atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg
     1681 gtgtgatgga caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc
     1741 taaatttgtg aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata
     1801 tatagaggaa gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga
     1861 tgatgtttta gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg
     1921 aatgatgtta gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt
     1981 cctactatgg gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa
     2041 aatgataaag ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat
     2101 ttaggtagtt atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca
     2161 acagcaatat ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt
     2221 aatataactg gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg
     2281 gtttgcactg ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga
     2341 gaaaaggatg cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat
     2401 atagacgttg tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga
     2461 aacacatact taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact
     2521 ttagctaagg aagctttaaa taatggagag agagacagct taaaaataat tgagataata
     2581 agtaaaaata taaatactta taatttggca aagattgatt atgtagaagt tgttgattct
     2641 ttatcattac aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt
     2701 ataggaaaaa caagattaat agataatttt acatttgaat tacaataggg ggctataatg
     2761 aaagttacaa tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat
     2821 tatgtaggaa gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat
     2881 gaaaaagtac aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct
     2941 ggagagagag gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact
     3001 ggagataaaa taattttaat ggcatattgt gaattcaatg aaaatgaggt taaggatcat
     3061 agacctaagg ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa
     3121 aaacatggaa ggttatcaga tataattctt taggttttgt aatgttattt atatatagct
     3181 agactattat tgaaatattt attgattact aattggatag atgtcaataa agtagacaca
     3241 aaaagagaag atacagactg tagacaaagt ccattttatg tgaatcggct acactagact
     3301 agacagaaaa aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca
     3361 agacgtcaac gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcaa tctctatctc
     3421 gctggtaagc ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat
     3481 aaatggatga agcaagcaca atcaacgggc tcattcaaaa aaagagacaa cttaacacca
     3541 gaacaagcag aattgatcgc actaagaaag aaaaataag
//
LOCUS       DACCCM010000148         3534 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCCM010000148 DACCCM010000000
VERSION     DACCCM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.156"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(184..741)
                     /locus_tag="IVK78_002799"
     CDS             complement(184..741)
                     /locus_tag="IVK78_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376189.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(920..1531)
                     /locus_tag="IVK78_002800"
     CDS             complement(920..1531)
                     /locus_tag="IVK78_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376190.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSARYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(1644..1901)
                     /locus_tag="IVK78_002801"
     CDS             complement(1644..1901)
                     /locus_tag="IVK78_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376191.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2498..2776)
                     /locus_tag="IVK78_002802"
     CDS             complement(2498..2776)
                     /locus_tag="IVK78_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376192.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(2810..3061)
                     /locus_tag="IVK78_002803"
     CDS             complement(2810..3061)
                     /locus_tag="IVK78_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376193.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1078 a          725 c          470 g         1261 t
ORIGIN      
        1 atttgccact ttgattcgta taaagctaac aactattaac tcctctcagc taatagttgc
       61 tctctttttt attcagattc atttttgctg atcacagcct agtatttcaa tggttttgaa
      121 cactaaacca aattgttcca ttacaacatt cactggctga tgaaatggat tagtagattt
      181 cgattattca aagcgatttt ctattggtgg atcgtgttcc caactaataa gacggattcc
      241 aaacctatcg tacagatctt ctaaaccact tataaatttt gcacctattt caggtgtttt
      301 attgcgtgga tattctattg ttacgccatt aaatggatac caattaggat taggctctct
      361 ttttttctgt acatctccca tagaatagtt agcactttct gggacataat atattcttcg
      421 accatcctct aattcatcta ataagatatt tcgtgatcgt ctatcacttg ctaaatgtag
      481 acgccacgtg cgataccatg ccatgctacc tgcatctgca gaaaatccat caaataatgc
      541 aacaattgta tcttctatca caatcctttc cccatctact tcatattcta atctaaaatc
      601 aaactcccct cgggtaattt ctggtcttgg tggcttagga cccaatatta ttctacttaa
      661 aaccgcattc gttcccagat gaaacacagc tataacaaca ataatgatta atattttgtt
      721 tctatttgat aaatttctca ttattcgcac atcacctcta cattctcaca tattagttcg
      781 taatactcct ttaatatcaa aatatctact ggtcgtaata atattcgcta tgaattccta
      841 ctactcgtat aagaaaggcc aaatcatcag cttttacttg ctgatgattt ggcttgtttc
      901 tatttatcgt aattgttttc taatcaccaa aattgttaac aatcggttca gatatttccc
      961 agttgattaa ggtaattccg tattgtgtca aaacttcttg cgcttctccg aaattaagta
     1021 cagttcttat gtatccactt tctcctaata taaagtctac ctctctttta aaaccttcct
     1081 cgagatttct aagggctata tgtggtttta atgacattga ttgtacattt tcccccatat
     1141 aatatcgcgc acttcctcga ctaaaataga ttcctagatc gtccccaaaa ggtagatctt
     1201 ctctaccact agctaatcta cttctccatc ttcgttctct tttcatagaa cctgcgctga
     1261 actcacaccc gttaaactca gctatgacag tatcttcaat tatgttaatc tgtccatcta
     1321 gttcgtattc aatcctaaat ggaaattctc cataggtgat ttccggttcg ggtattttag
     1381 gtgagattaa tgcaaagaaa ggaaatatta agcttggcaa catcaaaaaa actcctccta
     1441 tgatcacaat tcccgatgta atccaaaata taatttgtac aattctcatt gatccattgt
     1501 attccatttt attcccccta ttactattca tatccgccta tctatatcga tcttccaatg
     1561 cagagataat tgcttccata ccttgatttt ttactgagtt acctatgcga gatatcattc
     1621 tccactaagg tgcatcatat tgtctatcta acatgattaa ttctccaata ggtgtatact
     1681 cctccaaaaa caacatttaa tgcatctccc tcgacaacat aagctaccat gtttgctgta
     1741 tatgtgtttg agtctaattc ataagcagat aaatttactg tcgctggatt aaatataatc
     1801 gcattcgtat ttatagctac tgaatttgct gcttcagcac caccttttga atgtccgatc
     1861 attgtgattt caatagtagg atttacgtgg acagccctca ccgtttaccc tgaccattat
     1921 agaaatcgct tacttttttg aactacgcaa caaaggatac aatgctccta acttcactgt
     1981 ctccattttt gtccactaag gaatataaaa aataggcatt aattcattta attcggtcac
     2041 aacacgttac cttttaaatc attatactgt atctcataca ttattatcaa taaactatta
     2101 taaaaatgca aaaaatgctt tatcaatcta ttttgtaatt ggtcataata tcattgaaat
     2161 cataccctgt atcgattttt cctatgaatc tcgtctattt gtgattgacg aaaaagaggc
     2221 aagacagaca ctggaatggt aattttaccc actattttgc tccaactatt tgtaatcgta
     2281 cccacttttg aaataccgag atgatcattt taattgatat gctgattccc aagcaaaacc
     2341 ccaagaaatt aaatgctcta tcgcatctag cttcttgggt tttgcttata taaattccaa
     2401 catttaaatt ttttatgctg ttcggtctca gaaatgccgt ttttgcttat atcacaatat
     2461 caagatgtca aattattcgt gttgccgatg actttgatta atttttttca aattgtttat
     2521 taagactaat gctgtctttt tgcagatacc aatgttcgaa ctttttaaca ttatcatatg
     2581 atcctgcaag ttttaacttc ttaccattag atgttatcaa agttataatt aaccttgcct
     2641 ttttcccatt aggatctttt attttatgaa ttttctgcag gtaatctctc ctaatggcag
     2701 ctatttttac ctcatcagtg ctgataaacc attgatcatc cacatacata ctgcctgtta
     2761 tgttgtgttt ttccatttct tccgagaacg aaaagtttaa ggctcgttct tacttccaaa
     2821 gttgaatgag tccatggatt ggacttagtg tgagaattga gaacaacaat aagaatccat
     2881 atcctcccgc aaacatccct aaggctatac tgtctctcca tccataagta ggattagaag
     2941 agaaaagagt caccgtttgt aataatatga aaaggaataa tccagcacaa acaccaaaga
     3001 taacaactcc aaacattgaa tttttataca atgaccattt atttataggc ttttctttca
     3061 ttttacttcc ccctactgtt ctgttcacac ggaatgaatt caaagttcat aagccctcct
     3121 ctttgaacat atgaatacga cttgctaact atactttaaa aaagcttgtt ttaagcggtt
     3181 ataggcgagt tgtaatctct attcatctca ttagcaaata acttgccgaa aagtctactg
     3241 aaattaaagc agatagtgga agaattattt agccaggata gacactattt gtgaaacaca
     3301 tgtctttact tttgaaattc tatgatgaaa agccttggaa tacagtagct cttaattggt
     3361 aatacttttt ccattataaa taacctcttt tcattttata tttatcccct ctaaaaataa
     3421 tatctaatct aatattttaa acttatattt ccctcgctgt gctaatagag cattactaaa
     3481 taaatagaaa ctagaaaaaa gcattacgcc tgccagaata aaccaattat ttgt
//
LOCUS       DACCCM010000149         3460 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCCM010000149 DACCCM010000000
VERSION     DACCCM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.157"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..813
                     /locus_tag="IVK78_002804"
     CDS             <1..813
                     /locus_tag="IVK78_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6376194.1"
                     /translation="GKSTCAQMLGFESAVNEGKRTLIIDLDMQGNTSDVMNLTHMNFT
                     NEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPADMSFEMYDDWIKDQY
                     NDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIADWAIVVLQTQVKSMR
                     NAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQAQEIYGEHLIKNVVL
                     KNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            814..1362
                     /locus_tag="IVK78_002805"
     CDS             814..1362
                     /locus_tag="IVK78_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376195.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1622..2350
                     /locus_tag="IVK78_002806"
     CDS             1622..2350
                     /locus_tag="IVK78_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6376196.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            2380..>3460
                     /locus_tag="IVK78_002807"
     CDS             2380..>3460
                     /locus_tag="IVK78_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAP6376197.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT         1246 a          478 c          736 g         1000 t
ORIGIN      
        1 ggtaaaagta cgtgcgcgca aatgttaggg tttgaatcag cagttaatga aggaaaaaga
       61 accttgataa ttgacttgga tatgcaagga aatacttctg atgtgatgaa cttaacgcat
      121 atgaacttca ctaacgaaga aggaggcgga gaaggtgaac cgattgttta cgaaaatact
      181 gtgactgacg tgttgataaa caatcttcca cctaaagagg ctgtctataa agtaatcaat
      241 aacctctata tacttccagc agatatgtcc tttgaaatgt atgatgattg gatcaaagac
      301 caatataatg attcattatc ccaattcaaa tatatgaaag aaaagctaga accattattt
      361 gatgagtttg acgtcatcta tttagatgtc ccaccttcta tttctgtata tagtaaatcc
      421 gcgatgtaca ttgcggactg ggcaatcgtc gtcttacaga cccaagtaaa atcgatgaga
      481 aatgcgatgc aatatcttga gtatatggac ttttttgtga aagaatttga tacaaattta
      541 tatgttgctg gtgttattcc tttcatgttg gaaagcggtg acgcagtaga tcaggaaatg
      601 tataaacaag cccaagagat ttatggggaa catttgatta aaaatgttgt actcaaaaat
      661 gcgcgcttaa aacgatacga cggatcaggt attacgatgg agaagacaaa gaaaggtctc
      721 ttaaaacaat gggatcgcaa aagccacgaa ttatttatca gtattttgaa agaattggaa
      781 gaacacgaga attggtatgc ggaggtggag taattggctg acatcaaagg acttgatatt
      841 tttgggaaca acaaaggact cgccaaaata aaaaaagaag cagaagcaaa gaaaaagcag
      901 caagcaatta caccatctga aaataagcaa ttagaagtgc accaagttca agaaataaaa
      961 acttcctcca atgttgaaag acagagtaga ggaagaacaa aaaaagttgg cgcaccaatc
     1021 agaaagtttg ataaggttta ttccaccaag catcctttaa aactgtcgac tttgttaaat
     1081 gcgacttcca gagttttagt agaaaagtat gagactaata tgacaagaga tgaattgctt
     1141 agaaaagctt tagatgagta tattaaacag aatcttacgc aagaagataa gcaagatttg
     1201 tttaatgacg tggtgaaaga actagacttg ttcagagaaa aatatccaat tgttccagaa
     1261 ttagatgatc gtggagaaat aataagaact accagtcaga taaaacaaga taccgaagac
     1321 tcactgcgca aagggtgggg aatgaataat aaagttagat aataatcaaa gaagaactaa
     1381 gtaactacct ttaaacggta gttacttagt tcttctttga tttgcgtgaa atagagggga
     1441 tttatttttc tggttacgtg attaaaaaat ccaaaaaatg agcataggaa cggttatgat
     1501 tttagggtgt gttttaatga gatattgtga tttcattaac aagtgtatga taatctgttg
     1561 ttgagttagt aaataagtaa tagagctaac tctaaaataa aactattagg aggagatgta
     1621 ggtgaaaaag tttaaaaaga aaatatattt aggattggtt gttggggtga gtatgtttgg
     1681 gttcatggct catactaaac aagtaactgc agatactaac caaaaagaaa ggattatgag
     1741 ccaattagat gactctcaaa tagagtatgt acccaatgag ctaattgtca gtttaaaggc
     1801 tacacaaaga atttcaacag agaaaaataa accgcaaaat ttattgggtg taaaagttat
     1861 ggtggaaaaa gatttgactc ttaggacaaa tgaaagcgat tataataagc gagatcaagt
     1921 attattagtg aaattgaacg aagaaattag tgtggaagag gcagtaagaa catttaatgc
     1981 ttctgatatg gtcaattatg cagagccaaa ctatgtggtt gaactagagc cgttgaatga
     2041 tgttgaggag ccaaatacaa ctgattcttt aggaaatcga tcaattattc ttaacgatcc
     2101 taattggtgt aaacaagcta atttaagaga gattagcatg ccatccgctt gggaagaaac
     2161 caagggttct aaggatgtga aggtagcggt cattgattca ggaattgatt atcaacatga
     2221 agatctttct ggaaatgtag atgaaagttt gggatacgat ttttttgacg atgatacaga
     2281 tcctatggat actatgggac atggacacat attgccggga ttattggtgc tcaaacaaac
     2341 aacgaattag gtgttgctgg aataaattgg gatgttacga tggtacctat acgtgtattt
     2401 ggtagtagtg gaggtaccag tttggcaatc ttaactgaag ctattaatta tgcatcccaa
     2461 aaagggtttc caattatcaa ttttagtata ggtagtgttg tttatagcag tacagaaaaa
     2521 gcaattaaaa actatcctgg attattcgta gcagcagctg gaaataatgg gatagatatg
     2581 actgataaac ctttttaccc agcttctttg gatataccta atattattac tgtaggaaat
     2641 tctagtggtt atgaacgaag ttctgattca aattatagga aaacaggtgt tgatttattc
     2701 gctagagggt ttcgtgtttt tagtacattt ctaggaagta aatacacgta tatgtcaggt
     2761 acctcgatgg caacacctca tgtcactggt agtgctgcat tagcgttgtc actagatcct
     2821 gatctatcta ctgatgaatt aaaaaaaatc attttaaata gtgttgattc attttatgaa
     2881 ctatctgatt tatgtcttac agggggaaga ctaaatacga ataatgtcgt acgacaagta
     2941 acgggtaaac ttactgcatc taatatcaca ttaagattaa atagtgaatg gtcagatgaa
     3001 attgctaaag agatgtgtca cgttcactgg attgaccagt caggaaatga tttgacggat
     3061 caagtggttg ttctaaaaaa cgaagtggat acatctaact ttggcgatta cgaagtagtt
     3121 tttgcatcac ctgatttaac agatcaaatc tcagttacag tgaccgtacc tcgattacgt
     3181 aaacttacgc taggaaaaga aacagcaacc attgatattg gttccgcatg ggatagtgtc
     3241 atagccattg atacttttca agtgaaagct atagatgatt ttgggaatga tgttacaaat
     3301 caagtacagg ttactattaa tgctgatata gatacaatta attcaattca agcagtgatt
     3361 aagtttgaag ttccagaatt aggcttagcc caaattggta gactgaatat tactgcaaaa
     3421 aatagtacaa tcaatggatt gaatgaagtt agcatcgagc
//
LOCUS       DACCCM010000150         3402 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCCM010000150 DACCCM010000000
VERSION     DACCCM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.158"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(75..1073)
                     /locus_tag="IVK78_002808"
     CDS             complement(75..1073)
                     /locus_tag="IVK78_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6376198.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IVK78_002809"
     CDS             complement(1148..1444)
                     /locus_tag="IVK78_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6376199.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IVK78_002810"
     CDS             complement(1437..1730)
                     /locus_tag="IVK78_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376200.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IVK78_002811"
     CDS             complement(1876..2427)
                     /locus_tag="IVK78_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP6376201.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IVK78_002812"
     CDS             complement(2601..2900)
                     /locus_tag="IVK78_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP6376202.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3235)
                     /locus_tag="IVK78_002813"
     CDS             complement(2927..3235)
                     /locus_tag="IVK78_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6376203.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
BASE COUNT          990 a          664 c          464 g         1284 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttt aggaagtgta cctatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaagttgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga aacatttctt
     3241 ttccctcctt tgctataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa at
//
LOCUS       DACCCM010000151         3364 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCCM010000151 DACCCM010000000
VERSION     DACCCM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.159"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            3..587
                     /locus_tag="IVK78_002814"
     CDS             3..587
                     /locus_tag="IVK78_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6376204.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(664..1839)
                     /locus_tag="IVK78_002815"
     CDS             complement(664..1839)
                     /locus_tag="IVK78_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6376205.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1921..2301
                     /locus_tag="IVK78_002816"
     CDS             1921..2301
                     /locus_tag="IVK78_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP6376206.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2375..2737
                     /locus_tag="IVK78_002817"
     CDS             2375..2737
                     /locus_tag="IVK78_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376207.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1148 a          549 c          638 g         1029 t
ORIGIN      
        1 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
       61 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
      121 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
      181 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
      241 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
      301 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
      361 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
      421 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
      481 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
      541 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
      601 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
      661 gatttaatcg attgtctcta aagcatactt gattcgatta agtccttcca gtaaagtttc
      721 ttctggacaa gcaatattca agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc
      781 aggatttaat acgacttttc ctttccgaac aagagtgttc tccaattctt ggtcgtctga
      841 aatataggcg gtaaaatcta accacattaa gtaagttcct tcagggttca ttattctcac
      901 tttcggaaga tactgttcaa aaaactgatt aactgtttga acattttttt ctagataagg
      961 aatcagttgg cttaaccatt ctccacccgt ctcataagcc gcttgggttc caatcagtcc
     1021 aaaagtgttg atttcgtgct gttggttcat cactaaatgt ttttcaaaat cagctttcat
     1081 ctctggattt ttgataaata ccattgaatt tttgattgca gctagattga aagtcttggt
     1141 tgcggaagta aaagcaacta gtagatcatc taaattagga tcaatcgtta gcatggaagt
     1201 aaatgtatga tcgaataaag ttaaatcttg atggatctcg tcactaaaca gaatcacttg
     1261 atgttttaaa cacaattcgc ttaactgttt caattcttct ttgctccaaa cacgccctcc
     1321 aggattgtga ggattgcata aaatcattgc ttttacattg ttttcttcga tttttttctc
     1381 catgtcagac aagtccatga tgaaatgatt atttttttct agcaaagagc ttcgaacgat
     1441 gtgacgttga tttgtttcta taatagagga aaatggagga taaactgggt catgaatcaa
     1501 aatagaatcg cctggttttg taaaagtttg tactgctgca gctaaactgg ttaacacacc
     1561 acttgtaaaa acgatatttt ctttcgtcaa ctccacagca tggtgttggc gttcccaatg
     1621 aatgatagcc tgatatagtt tatcagacag agtggcataa ccaaagattc catgtttgat
     1681 gtattcacta aacgctttag ctaccccacc gggagacaag aagtccatat ctgctaccca
     1741 caaaggaagt aagtcatcca tttgatatgt ctcagcaatt gcgtcccatt ttacactttc
     1801 tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt
     1861 cgtcgcttct ttttttgagc tatttacagt ataatgataa atgcgaagag agggaagtag
     1921 atgacttata aaattggaca aataattgaa ggaaaagtga cagggataca gccttatggc
     1981 gcattcgttt ctcttgataa cgagacgcaa ggacttattc atgtttcaga agtacaatcc
     2041 ggttatacga aaaatatcca ttcattgtta aaagtcggac agccagtaac agttcagatc
     2101 atcgacattg atgaatattc caaaaaaatc agtttatctt taagaacgtt agaaaaaaca
     2161 aatccagctg ttccatatcg cagaaaaagg tatttcacga ataaaaatcg taagatcgga
     2221 tttaccacta ttgcggagca gttacctatt tggatagaag aagcattatc tgaactagaa
     2281 cggaaagaaa ataagaaata aaaagtgtat tttttctgaa aatgtgctag aatttttatg
     2341 taagccatag aacaagttga tcgaggtgga aattttggca agcaaaagag ttgctggtac
     2401 aattatgttg catttggagg atggtactaa aaaatttttg gtccattcaa ttgatgacaa
     2461 gttggaattt gcgttagcgg aactttctga aggaaagact ggtttagcga atatacttaa
     2521 tttcttaaaa gaaatagttc atatcgatgt tagcaagatt agtttgatgg aattgacaaa
     2581 tacacatatt aaagaggaaa atgttccgtt gtttgtcttt gaaactgaac aaaagaatca
     2641 aagagaagaa ttaggcgaag gatatatttg ggaagaacca ggtcaaatgc gctctgttct
     2701 tggaacttac gaagtagagg gagtgccttt cttctaaaat acagattaag cgaacaaaga
     2761 agtttgaatt tttttcaaac ttcttttttt attgtttggt ccaaaaaata caatgaaatg
     2821 ttctgctaaa gatagttttt tagtgcattt tattttcaaa atatgttgaa aacggttgta
     2881 taacgaataa taataattga tcgaaaaatc atttcacaaa gtaatgctta atttatagtt
     2941 gaccaaggaa gaaatacatg ataaatttaa cagcgttgat tctgaagtgg atgttatatc
     3001 acatttctaa aaaatttaaa aaatagttat tgacatgtta ctaacgacat gataagataa
     3061 ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa
     3121 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
     3181 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gtttgtctct ctgatatgat
     3241 ataaaagttg ctacggcagc caaaaatgaa tcgggaaaca ctctcgatga agtagacctt
     3301 tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc aattcaagac
     3361 aata
//
LOCUS       DACCCM010000152         3176 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCCM010000152 DACCCM010000000
VERSION     DACCCM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.161"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            204..1172
                     /locus_tag="IVK78_002818"
     CDS             204..1172
                     /locus_tag="IVK78_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6376208.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IVK78_002819"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IVK78_002819"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6376209.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IVK78_002820"
     CDS             2202..2810
                     /locus_tag="IVK78_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6376210.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          940 a          609 c          772 g          855 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gcgatattgc
     2941 ggtcattatc tgcgtgcgct gcggcaagat agcctgataa taagactgat cgcatagagg
     3001 ggtggtattt cacaccgccc attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg
     3061 gtatcactta tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata
     3121 attgactgta atttacgggg caaaacggca caatctcaaa cgagattgtg ccgttt
//
LOCUS       DACCCM010000153         3024 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCCM010000153 DACCCM010000000
VERSION     DACCCM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.162"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1263
                     /locus_tag="IVK78_002821"
     CDS             <1..1263
                     /locus_tag="IVK78_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6376211.1"
                     /translation="EGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIG
                     GTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPV
                     EVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGN
                     KKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKE
                     DLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPI
                     TEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDES
                     GNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDS
                     NIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1397..2740
                     /locus_tag="IVK78_002822"
     CDS             1397..2740
                     /locus_tag="IVK78_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6376212.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2852..>3024
                     /locus_tag="IVK78_002823"
     CDS             2852..>3024
                     /locus_tag="IVK78_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6376213.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1201 a          407 c          573 g          843 t
ORIGIN      
        1 gaaggtgtca ttgaagtaac gtatccagac ggtacgaaag atacggtgaa agttccagta
       61 gaagtgacag acaatcgttc tgacgccgat aaatatgaac caacagtaga aggcgaaaaa
      121 gtagaaattg gaggcacagt agatttaaca gataatgtta ctaacttgcc agcgttacca
      181 gaaggaacaa cagtaacaga tgttactccc ggcggtacaa ttgataccaa cacaccaggc
      241 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
      301 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      361 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      421 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      481 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      541 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      601 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      661 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      721 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      781 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      841 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      901 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      961 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
     1021 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
     1081 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
     1141 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
     1201 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1261 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1321 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1381 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1441 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1501 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1561 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1621 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1681 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1741 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1801 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1861 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1921 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1981 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     2041 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     2101 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     2161 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     2221 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2281 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2341 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2401 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2461 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2521 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2581 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2641 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2701 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2761 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2821 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2881 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2941 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     3001 aacagcagag agtagtgaac tttt
//
LOCUS       DACCCM010000154         2850 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCCM010000154 DACCCM010000000
VERSION     DACCCM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.163"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..131)
                     /locus_tag="IVK78_002824"
     CDS             complement(<1..131)
                     /locus_tag="IVK78_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6376214.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
     gene            complement(205..486)
                     /locus_tag="IVK78_002825"
                     /pseudo
     CDS             complement(205..486)
                     /locus_tag="IVK78_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            948..2330
                     /locus_tag="IVK78_002826"
                     /pseudo
     CDS             948..2330
                     /locus_tag="IVK78_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2566..>2850)
                     /locus_tag="IVK78_002827"
     CDS             complement(2566..>2850)
                     /locus_tag="IVK78_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6376215.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT         1001 a          514 c          480 g          855 t
ORIGIN      
        1 tgacaaacat ttattccacg atcatataac aattcctgaa cttcacgata gcttagattg
       61 taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg tttgccttta
      121 aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta aaatagattt
      181 tttggaaaac tttgcaacag aaccgaaatg agtatagtat gaattagtaa tttggttttc
      241 atctaagttt agctttgatt tactaaaatt gatatctcga tacatgttca atacatctaa
      301 tacaaatcga gaatcttctt tcggaaatgg ctcagaaaga tcactccaaa tttcatcatc
      361 ataatgatac tcgtaacctt ctctcaatat ttcgattttt ctttcataat aagatctgtt
      421 ttggtcctca aatttagcca ataactcaaa ttgatttact agtgacaatc tctgaaaact
      481 gtccattaaa tgtttcttcc tatcttgttc attaacaaaa aattataagc ccttacaacg
      541 aatattatat attaaattta attgtaggtg aacacgaatt ttataaatat ttatggtgtt
      601 aatgacaaat gatatccgac tcctcttcat acgttctgga ctatttgcta atagattaaa
      661 atttcagcat ctatttctga ccataaataa atgagctgcc gaaaagctgc caaatcagac
      721 ttgaaaataa aggatcaaaa acttttaagt ttctgacccc gtaactcaca cttcaaaatc
      781 ttacttaaaa aggagtctga aagaaaaaaa gagtattcaa aatttttttc taactagcta
      841 catcacatct atgcattgac actcaaagaa ccagaataag tcatcagaaa gcaagggggt
      901 gcggacataa tcctggacga aaaaataaac ttaggaggaa tgtccgtgtg ttttcccaca
      961 aagagcgtgt aagggcaatt cagttatttt taaaatacga ttgctcttat gctgcaacca
     1021 ttcgagaatt agggtatccg tcgattggtg cactacggaa atggtataaa gagtatttgg
     1081 aatccggaga gctccatcag gagcaccgaa aaaaatcaaa gtattctgaa gaacaaaaac
     1141 gaatagcggt gaatcattat tttgaatatg gtcaatgtta tgcacgaacc attcgcatgc
     1201 ttggttatcc taatagagag tccttgcggc agcggtgtga agaacttgca ccaggagcta
     1261 gaaaactccg aaaaagtgcg gtaaagttaa cacaagatca aaaagaagct gtgttgaaaa
     1321 aattctataa accgcaaact aataggaaaa atttagccga atctggagga attagtagag
     1381 ttactctata tcaatggaaa aacacggttc ttggaaagga ttttccattg tgtatgactt
     1441 taaaaaatca ggaaaaacaa aaagagctac ttttaaagga agtagaagac ttacaaaaac
     1501 aagtacacca attacagctc gaaaaagcat tactcgaagg agcggctgaa ctgctaaaaa
     1561 aagaaaaagg cgtcaatctt ctttgtctat caaatcaaga aaaaacgata ttgattgatg
     1621 ccttacgaaa tcagttcacc ttgaaagagc ttcttcaaca gctccagttt cctaaaagca
     1681 gctattttta tcaaaaacaa gcacttgaga aaccagataa gtattacaaa gaacgtcaaa
     1741 ttaattatca cgattttcaa tcataacttc tgtgcgtatg gttaccgtag aattcaccaa
     1801 gctttaaaaa atatgggtaa aaagctttct gaaaaagttg tacgtagatt gatgactgag
     1861 gaaaatctat ttgttaaatt ttctagacga aaaaagtatt cttcgtatgc tggggaaatt
     1921 tctcctgcac accctaattt attaaatcgg aattttaaag caaagaagcc aaatgaaaaa
     1981 tggttaacag atattactga atttagaatt ctcgcaggga aaatctactt atcaccactt
     2041 gtcgattgtt acgatggtgc tattgttagt tggactattg gaacaaagcc aacttgcttc
     2101 actctatgtc taaaaagggt tattcaccag ataattcagc gtgcgaaggt ttctttggac
     2161 gattaaaaaa cgagatgttt tatcaacgtg actggaagaa tacaacaatc aatcagttta
     2221 taaacaaatt ggatgattat attcattggt ataataatca acgaattaag ttatctttag
     2281 gagggctaag ccctcttcaa tatcggaaga agcaaagata tatttgttaa aaaggtccaa
     2341 aaaaaatatc cgcaccccct tttttttgta aaaaactatt gcacaataca agtagcctac
     2401 ggctatcgaa atttccataa ctttcgagca agaacttatc tcattcaagg cttgatcttc
     2461 caagtaaaac aaaaaccagt taagcactct gcctaactgg ttatgactaa attttcagtt
     2521 gtatctttct ctaccaacac taaatgtcga agagcctttt ttcgtttatg aatgtaagac
     2581 cgcttctcga gccaatgcca aacaaccgag cgttccagcg ttgtcttcta atgctggggt
     2641 cacgatatat ttgcttaact caggtgtttc gacataccca ttgaccaatt ttgcaaatgc
     2701 gtcgtggacg ttttgcagca tatggcgttg tttcatcaca ccaccgccaa aaatgatcac
     2761 atctggagaa aacatcaagg ttgtattgta ggcacattga gcaatatagg aggcttcgat
     2821 ttcccaataa ggatcatctt ctgcaacctc
//
LOCUS       DACCCM010000155         2797 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCCM010000155 DACCCM010000000
VERSION     DACCCM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.164"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            6..305
                     /locus_tag="IVK78_002828"
     CDS             6..305
                     /locus_tag="IVK78_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6376216.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVK78_002829"
     CDS             378..1739
                     /locus_tag="IVK78_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6376217.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVK78_002830"
     CDS             1761..2021
                     /locus_tag="IVK78_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376218.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVK78_002831"
     CDS             2035..2787
                     /locus_tag="IVK78_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP6376219.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
BASE COUNT          873 a          443 c          629 g          852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgac
//
LOCUS       DACCCM010000156         2640 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCCM010000156 DACCCM010000000
VERSION     DACCCM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.165"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            112..1311
                     /locus_tag="IVK78_002832"
     CDS             112..1311
                     /locus_tag="IVK78_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6376220.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1338..2606)
                     /locus_tag="IVK78_002833"
     CDS             complement(1338..2606)
                     /locus_tag="IVK78_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP6376221.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT          791 a          573 c          469 g          807 t
ORIGIN      
        1 ataagaaaaa catctttcca tcggaaagat gtttttttgc tttttctagt ttaaataaca
       61 gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt tatgaatcta
      121 accagagatc tgatcttttt agttctctat cttattgggg tattctctat cgtttattgg
      181 cgaaaacaga agaaggatcc ttccttgttc ttttacggca gtacgtggac atttacggta
      241 atcttaactt attttgtcgt gttggaaatt ttttattcga ccttttattt tttgattcca
      301 ttgttgttct ttagtatctt tgctttttct tatttcacag aaaaacgaag gctactgaat
      361 ggtcttttat tcaatgtttt actcatcagt ttcggcatct acttgtttgt cttgctgtat
      421 gaaacgcaaa atatttttct tggcggactg atcgccttga tcacgattcc attgctactt
      481 gtccttttat ttggtattta tggattgatt gtttttcttt tttggaacgg ggtcacagtt
      541 ttacgtagag aatcgcattc attagctaat cttttaacgt tgatactagc catttttctt
      601 acactgtttc ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg gataaatgct
      661 ttgtttttct gcgttcccct gatcttgatc tatctattta tcgtattcta caactttttg
      721 actgtctcat tcttgtatca gttcaaccgg cctcgataca atcaagactt catcgttgtg
      781 ttaggcgctg gattgatcaa tggagaaaca gtttctcctc ttctagccaa gcgaatcaat
      841 aaggcaattg ctttttatcg ggctcaaagc cgtgctacat taaatccgcc catcttattg
      901 atgtctggcg ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat gaaacaatat
      961 gccatggaac aaggaatccc tgaaagagac attcttgtcg aaaccaattc gacaaccact
     1021 ttggaaaaca tgctctattc aaaggaaatc atggatcagc aaatgaaagg gccataccgc
     1081 gcgatcttta gttccaacaa ttaccacatt tttagagccg gtctttatgc gcggcaagcc
     1141 aaattgaaag caaatggtat tggtgcaaaa acagctttct actacttgcc taatgcgttt
     1201 ttaagagaat acctcgccat cttgatgatg aacaaaaaaa gacatatgat catttgcgga
     1261 ttgcttttcg caggctccgc attcttatcg ctgattactt tacttttcta aaaaaacaag
     1321 gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac tattggcaaa
     1381 aagagtatcc cttttttgac cgctacttgc tgagcagaat aaataccgct gttgcctgaa
     1441 cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga accgaacaat
     1501 tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa tactccgata
     1561 aagcccaatc ctgcaagaaa aggaacagaa acatggagca aaggcgctag tgctgcacca
     1621 agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc cgcgcctaaa
     1681 gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg tccattaaat
     1741 gattcttgaa gtaacggaag actcaatccg agataacgtt gtgtttgcaa aatcaatgct
     1801 gctgccacaa ctacagctcc acctatcgta tttcgacaaa caggatgagg aatccattgt
     1861 gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat caaaccaaaa
     1921 gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg aatcacaccc
     1981 atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc aaacagtgca
     2041 gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa cacttctaat
     2101 ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc actgattcca
     2161 ctgataatca agatttcccg ttctgattta tccaatcgga acaaacgtgc cgtattatct
     2221 gctaacgctc cccccatttg gactgctgtt ccttcacggc caacagatcc cccaaataaa
     2281 tgggtagtta tggttccaaa cagtgtaagt ggaataagcc gcaacgggat tttttcttct
     2341 ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagtcc gccgtgatac
     2401 gcatagagat aagcaaaaag cgcaccacta actggtagta gacaaaggag ccaaggatgt
     2461 gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga caagctcccc
     2521 atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatatacac taaaatgcgt
     2581 actggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa acgcctacta
//
LOCUS       DACCCM010000157         2592 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCCM010000157 DACCCM010000000
VERSION     DACCCM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.166"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            120..626
                     /locus_tag="IVK78_002834"
     CDS             120..626
                     /locus_tag="IVK78_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP6376222.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="IVK78_002835"
     CDS             643..1542
                     /locus_tag="IVK78_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP6376223.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2514
                     /locus_tag="IVK78_002836"
                     /pseudo
     CDS             1629..2514
                     /locus_tag="IVK78_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT          941 a          480 c          508 g          663 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccttgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcaactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaag gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacttat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtca tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcaat
     1861 catcacaatc aacacaagac agtacagaag caatccaaga cccgactgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacgcc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgtc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa aagtcactgg caaaaccccc gcttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caaggaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 gggtcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca aggctatcgc atcacgacag ttagcgattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aa
//
LOCUS       DACCCM010000158         2542 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCCM010000158 DACCCM010000000
VERSION     DACCCM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.167"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(452..751)
                     /locus_tag="IVK78_002837"
     CDS             complement(452..751)
                     /locus_tag="IVK78_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6376224.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            1102..1734
                     /locus_tag="IVK78_002838"
     CDS             1102..1734
                     /locus_tag="IVK78_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6376225.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1857..2495
                     /locus_tag="IVK78_002839"
     CDS             1857..2495
                     /locus_tag="IVK78_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6376226.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          851 a          406 c          343 g          942 t
ORIGIN      
        1 atcatctctc aaaaatcctt tcttcaattc ctcccccact catttccaat cttttaacta
       61 aaaaattgat agttatttta caaatattta taaaaaataa atatgccaaa aaactgtttt
      121 tacaaggttt ttcctaatgt aaattatttt ttcgcatata ttcattttat tttacttatt
      181 gctttcattt tttcaatcta tatttccaat ttttaactta caaaaatgca acattaataa
      241 ataacgatag tttcataaaa tacactttta attaacatta ttagtttata ctagctgttt
      301 ttgtgttaat taaaggataa aatacaatat aaagaaacat ctgtttcttt atttagaata
      361 aaaaaataca gactctgttc aaataaatga acctagcctg cattatgaga ttgactatac
      421 tttttaattc attgagatga ataactcgtt tttataaatg cccaaaagta atcggtgccg
      481 aagccattcc tcgacctaat tctttttcaa attttcctat tgttgatatc tccatataaa
      541 acggtgatac tggtttagac aattctcccc tgattgctaa cggacgtaaa ctacgttcca
      601 aattatttat gactttagga taatactgac ctttttctaa gcctgtcttt gcgtctatca
      661 aaagtatacg ttccttttct ttgatctctg gatttaaaac gagatcatat aattttagta
      721 ccatgttttc tatttcttca cttttagaca tcacgatttc ctcccaaaaa tatatttctt
      781 cccactacat tctataaaca aagtatacgc tacatctaga tttttttaaa tcattactgg
      841 attgttaaaa cctttcaata cactgttatg gcaacgattt ttattttaaa aacattctaa
      901 attaaatcag taaaaatttt tattctaaga tttttattag aattacgcct aatttgtgtc
      961 tctcagggca attctaagaa cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc
     1021 gaaaacctat ctcattttct gctaatgtaa gttggtttag cttttgttaa tatgacttta
     1081 tgaaaatgga ggtaattaaa tatgattttt cctaaagtta atgaagcaag cttgaataaa
     1141 ggatcctttt ttcatatcta tgaaaacgaa gattttgaac tgtataatat cctttcacaa
     1201 aaagaggtca tcgaaaacca aataatctgc catccaatta tttacaaatc tattttcaag
     1261 tatgcatttt ttgcttccaa tgattttcaa agaatggaga ttaccgactg tatattcgaa
     1321 aactgtgatt tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc
     1381 aattgtaaat taacaggtac taaattgatg gaaagttata ttgggaacac gttattcgag
     1441 aattgtaaaa tggactatgt aaatttatct ggttcaaaca taaaagaaag tacctttgag
     1501 cactctgtct tatcaagcgc agattttgta gactgttccc tcactaaaat aatgttttct
     1561 actaatgatt tagaaaatgc tagctttttt gatactgatt taaaaaatat cgatttaagt
     1621 agcaactcct ttgaaatgat tgaagtacgc tcggaacatc taaaaaattg tacgcttaat
     1681 cagttgcaag cacttggttt tgctagaaaa tttttacaaa taaaagtagt ataatcaaaa
     1741 tgaatatata agataaattt ttagcaatac aaatatccat aaaaaaacat tgcctatttt
     1801 aactcttcat gtatactgac tctatcttca aaaactgaat agtaggagtt attatcatga
     1861 ataacatttt aggaaaaaaa atagaactta aaaaagggat attgggcact attgtcgttg
     1921 tactcggttt gttagggttg ctatttgcgc catccattat tagcattttt tctttaacta
     1981 tcactggatt tatagcattt acacttgttt ttggtaaaaa agatacgaaa aaaatgttct
     2041 cgaaacctgt cgctcctgtc aaaaatatag ccaaatattt ttttatcaat gtagtaatca
     2101 gtgccgcagt ctcggtattt ttgcaacaaa ttttgaaatg gggtttaaca ggcaatccaa
     2161 taaatgaggc tttcagcccg ttattattta ttattttgcc tatcatgata cttggagaag
     2221 aattgttctc agtgtatttt ctcgctattt tttctagtaa gttcagcatt cctgtcgcta
     2281 gtttcttgtc agcaatcatc tttggattta tccattacag tacttatgat aatggaaata
     2341 ttttacacac tgtagcacac atccttttga tacaaggggt ggcaagattg ttattcaacc
     2401 aagctgccat taaaagcaac tcgatcatta caagttgggc agttcatgtg atatttgatt
     2461 tcacagctat attgctagtc gttttattct cctaaatttt tatagaaata atacatgctc
     2521 tattgctaag aaagcatact tt
//
LOCUS       DACCCM010000159         2483 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCCM010000159 DACCCM010000000
VERSION     DACCCM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.168"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1014..1304)
                     /locus_tag="IVK78_002840"
     CDS             complement(1014..1304)
                     /locus_tag="IVK78_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376227.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IVK78_002841"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IVK78_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACCCM010000160         2468 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCCM010000160 DACCCM010000000
VERSION     DACCCM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.169"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(493..1785)
                     /locus_tag="IVK78_002842"
     CDS             complement(493..1785)
                     /locus_tag="IVK78_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6376228.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IVK78_002843"
     CDS             complement(2059..2319)
                     /locus_tag="IVK78_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376229.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DACCCM010000161         2419 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCCM010000161 DACCCM010000000
VERSION     DACCCM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.170"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            5..1150
                     /locus_tag="IVK78_002844"
     CDS             5..1150
                     /locus_tag="IVK78_002844"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP6376230.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IVK78_002845"
     CDS             1321..1779
                     /locus_tag="IVK78_002845"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP6376231.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IVK78_002846"
     CDS             1938..2408
                     /locus_tag="IVK78_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP6376232.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          700 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DACCCM010000162         2397 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCCM010000162 DACCCM010000000
VERSION     DACCCM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.171"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            206..2068
                     /locus_tag="IVK78_002847"
     CDS             206..2068
                     /locus_tag="IVK78_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6376233.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IVK78_002848"
     CDS             2068..>2397
                     /locus_tag="IVK78_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6376234.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACCCM010000163         2349 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCCM010000163 DACCCM010000000
VERSION     DACCCM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.172"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            13..251
                     /locus_tag="IVK78_002849"
                     /pseudo
     CDS             13..251
                     /locus_tag="IVK78_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            253..1551
                     /locus_tag="IVK78_002850"
     CDS             253..1551
                     /locus_tag="IVK78_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6376235.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2198..2349
                     /locus_tag="IVK78_002851"
                     /pseudo
     CDS             2198..2349
                     /locus_tag="IVK78_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          836 a          400 c          429 g          684 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa atagggttct gttgcaaagt tttccaaaaa atctatttta
     2161 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     2221 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     2281 tcgttaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     2341 tcgttgggt
//
LOCUS       DACCCM010000164         2325 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCCM010000164 DACCCM010000000
VERSION     DACCCM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.173"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..336
                     /locus_tag="IVK78_002852"
     CDS             1..336
                     /locus_tag="IVK78_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376236.1"
                     /translation="MQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRFGFESFADF
                     ERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNEYDGLLGVMPSKKSQMNESKFM
                     SIFNDVNLR"
     gene            558..1964
                     /locus_tag="IVK78_002853"
     CDS             558..1964
                     /locus_tag="IVK78_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6376237.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELGYISRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKKAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT          796 a          348 c          552 g          629 t
ORIGIN      
        1 gtgcaagaag aaaaattaaa aaaagaacag gcgaaacttc gccaacagaa gaaacgattg
       61 attgagaaag aaaatgctgc aatgggcaag gctcttcgca agcgttttgg gtttgaatct
      121 tttgctgatt ttgagcggtg gttatcgtca atttctgacg atcagcaaca cgaaaaatca
      181 gtggataatg agtggcttgc tgatgaaaaa tcagcgccaa gcgaacgaga ttatcccaat
      241 gaatatgacg gcttactcgg agtgatgccg agtaaaaaaa gtcagatgaa tgagagtaaa
      301 tttatgtcaa tttttaatga cgtgaattta cgataagcta ttattttgcg tgaagcaaaa
      361 taaagcgtaa aaggagttga gagcgtgaca cgctttcggc tccttttttc gttgtcactt
      421 gtgacagcga aatccctcgg agagcgtttg gaattgtgac agtcatgtca caattaggaa
      481 aacgttactc tttgacaaag agtaacagaa aggtttacac tctttttatt agcgacagcg
      541 cgaaggaggg gtctaacttg gggtttagta tttcgttcaa aaagggaaca aagaatacaa
      601 gcatttctca taataatcgt gagttaactg atagccaaaa aatcaatgac tggcataagc
      661 atattgattt cacaaaatcg gatgaaaata tctatttgga acaaacaaat attcgtgaaa
      721 aatatgaaga attattcggt gaagccgtag aaaaatataa tgcaaagcag aaaagagccg
      781 atagaaaaat cgaggactat ttagcaaagg ttcgcaagga taaaaaattg gaaccacagc
      841 gagaatttat cgtgcaaatt ggtactcttg atgattttcg tactactcgt gatgatggtt
      901 cttctactgg catatcggaa caacaagcag aacaaaatcg agtgattgcc aacaaaattt
      961 tagttgagta tttcaaagaa tttaaagaac ggaatcctag tttatccgtc tacaatgctg
     1021 ttattcataa tgacgaagta agtccgcacc tgcatttgaa tattgttcct gttgctgaag
     1081 ggtataaacg gggtgttcaa aagcaaccaa gttttaacaa agccttgcaa cagcagggtt
     1141 tacgagcaga taaagagaat ggtcgagtga tttggaataa ttttaggaat caggaagttg
     1201 agtcaattga acgtttgatg gcagattttg gttgggagcg tgagttagtc ggtactaaca
     1261 aaatcaaaga tgttcacgaa tataaagaaa tcatgagcga gatttcagaa ttgcgtcaaa
     1321 cggccgtaaa agagcgtgag agcgtttctg atgagttggg gtatatttct aggcaaagag
     1381 aagaaaacga gcgagaaaag gctctggtgg cttctgagag agcaaagatt gaagaagaga
     1441 agaaaaatat gctgaatgta aaaaaagaga tggcggtcat tttccaaaaa gatattgttc
     1501 ctttagaaag tttcaagagg cttcctgatg gttcgtatca tttatctggg aatgattttg
     1561 ttgatttgta tcaacgagct acgagagcaa caaatgccaa gagaaatgct gataacatga
     1621 gtgagcaata tttacagatg cttgagcaaa atacagaatt aaatgagtat gtagctacat
     1681 tggaagaaga gcgtcatttg tctggtagaa aacaattagc agatttacaa gtggaaaata
     1741 aaaagctgaa agaagaaaat cgctctttac gccagcgttt agagcgatta aaaaaagcat
     1801 ttcaaaaatc gatcactcgt ttgtctatcc gttttggtct tgcagaaaag gatatggggc
     1861 tttctgagga attagaagta ttagagcagg agaaaacctt attttatcgt caaaacgtct
     1921 cacagcataa attagagcgt tctgaagatg agatcgagtg gtagttacga tgtgcagtca
     1981 acggtaccgg aggcggtctg tgccgaggat aaggagttga cggctaaacc actagcctat
     2041 taagctgaca tttgctaaaa aatggcaagt ccattttttg gccaacctag cgttagcgaa
     2101 agtttggggg cgcccttttc aaagggagcg gttttatttt tgcatgacta agaatgagtg
     2161 ggaaaaactt aaccgcattt ctaacaggcg gttaaagtat caaagtttga cccttccctt
     2221 aaaaatgcag tggtcaattg accgaatttt atgcagaagt gggtttttga gttttggggt
     2281 ttgaaacgag aaaaaagagg tcgatttcga cctctttttt tgatg
//
LOCUS       DACCCM010000165         2211 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCCM010000165 DACCCM010000000
VERSION     DACCCM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.175"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVK78_002854"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVK78_002854"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP6376238.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          589 a          479 c          404 g          739 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACCCM010000166         2165 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCCM010000166 DACCCM010000000
VERSION     DACCCM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.176"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..112
                     /locus_tag="IVK78_002855"
     CDS             <1..112
                     /locus_tag="IVK78_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6376239.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            326..1021
                     /gene="vanR"
                     /locus_tag="IVK78_002856"
     CDS             326..1021
                     /gene="vanR"
                     /locus_tag="IVK78_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6376240.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            999..2153
                     /locus_tag="IVK78_002857"
     CDS             999..2153
                     /locus_tag="IVK78_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6376241.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          730 a          400 c          461 g          574 t
ORIGIN      
        1 ttatgcggta aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac
       61 taatgtatct agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg
      121 tattccgcta atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc
      181 tcctagcgat gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta
      241 gtcatttatt atgtaaatgc ttataaattc ggccctataa tctgataaat tattaagggc
      301 aaacttatgt gaaagggtga taactatgag cgataaaata cttattgtgg atgatgaaca
      361 tgaaattgcc gatttggttg aattatactt aaaaaacgag aattatacgg ttttcaaata
      421 ctataccgcc aaagaagcat tggaatgtat agacaagtct gagattgacc ttgccatatt
      481 ggacatcatg cttcccggca caagcggcct tactatctgt caaaaaataa gggacaagca
      541 cacctatccg attatcatgc tgaccgggaa agatacagag gtagataaaa ttacagggtt
      601 aacaatcggc gcggatgatt atataacgaa gccctttcgc ccactggagt taattgctcg
      661 ggtaaaggcc cagttgcgcc gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa
      721 tgttatcgtc cactccggcc ttgtcattaa tgttaacacc catgagtgtt atctgaacga
      781 gaagcagtta tcccttactc ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa
      841 ggggaatgtg gttagctccg agctgctatt tcatgagata tggggcgacg aatatttcag
      901 caagagcaac aacaccatca ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac
      961 cattgataat ccgaaatata taaaaacggt atggggggtt ggttataaaa ttgaaaaata
     1021 aaaaaaacga ctattccaaa ctagaacgaa aactttacat gtatatcgtt gcaattgttg
     1081 tggtagcaat tgtattcgtg ttgtatattc gttcaatgat ccgagggaaa cttggggatt
     1141 ggatcttaag tattttggaa aacaaatatg acttaaatca cctggacgcg atgaaattat
     1201 atcaatattc catacggaac aatatagata tctttattta tgtggcgatt gtcattagta
     1261 ttcttattct atgtcgcgtc atgctttcaa aattcgcaaa atactttgac gagataaata
     1321 ccggcattga tgtacttatt cagaacgaag ataaacaaat tgagctttct gcggaaatgg
     1381 atgttatgga acaaaagctc aacacattaa aacggactct ggaaaagcga gagcaggatg
     1441 caaagctggc cgaacaaaga aaaaatgacg ttgttatgta cttggcgcac gatattaaaa
     1501 cgccccttac atccattatc ggttatttga gcctgcttga cgaggctcca gacatgccgg
     1561 tagatcaaaa ggcaaagtat gtgcatatca cgttggacaa agcgtatcga ctcgaacagc
     1621 taatcgacga gttttttgag attacacggt ataacctaca aacgataacg ctaacaaaaa
     1681 cgcacataga cctatactat atgctggtgc agatgaccga tgaattttat cctcagcttt
     1741 ccgcacatgg aaaacaggcg gttattcacg cccccgagga tctgaccgtg tccggcgacc
     1801 ctgataaact cgcgagagtc tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg
     1861 ataacagcat cattgacatt accgcgggcc tctccgggga tgtggtgtca atcgaattca
     1921 agaacactgg aagcatccca aaagataagc tagctgccat atttgaaaag ttctataggc
     1981 tggacaatgc tcgttcttcc gatacgggtg gcgcgggact tggattggcg attgcaaaag
     2041 aaattattgt tcagcatgga gggcagattt acgcggaaag caatgataac tatacgacgt
     2101 ttagggtaga gcttccagcg atgccagact tggttgataa aaggaggtcc taagagatgt
     2161 atata
//
LOCUS       DACCCM010000167         1980 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCCM010000167 DACCCM010000000
VERSION     DACCCM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.177"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(325..529)
                     /locus_tag="IVK78_002858"
                     /pseudo
     CDS             complement(325..529)
                     /locus_tag="IVK78_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(729..1745)
                     /locus_tag="IVK78_002859"
     CDS             complement(729..1745)
                     /locus_tag="IVK78_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6376242.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          558 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaaa attttcctaa ttgtgacatg gctgtcacaa
      541 ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa ggagccgaaa
      601 gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcatgcaaaa tattcgctta
      661 tcgtgaattt acgtcactaa acattgacgc aaatccactc tcactcatct gacttttctt
      721 attcggtatc acttctctgt cacatccaac cccaaaagcc acgtatccaa ttcttttttt
      781 aattcgtctg cagattcctt aaacgtctct cgtaaatgat tcgttaactc atcattttga
      841 ctttcttgct tcagcaaatt ccgatattta tattttgtat gtctagcaat tatcgaccaa
      901 ttagaaggct cagctatcaa accatcaata actattttat caataggttt tatatcaatc
      961 ggcaaaaaat gcgggcttgc aaaactatct aagctttcct gaaccatcgc atgccaatcc
     1021 gttgctttgc cacgtcttag ctgcatttct aaccgccacc acgtatcgat ttcttttggc
     1081 acgattactt tttttcgttc ctgctctagc tttttgttgt acagtcgcac ttggcgctca
     1141 ctggcacgac tgccccagta cgctgtttct agcttgccag aacggccgta gattggctta
     1201 aaagaaatcg ggtctacaat acgatactga gtaataaagt catccggcac atctacaata
     1261 tcgcaggcaa tatccgcccg ggaaaaatgc ggatctaaaa ataggtcatg aatgaatcct
     1321 ttcatggaac tagctaaaaa ctggcctatc ttattcggat taaaatcaat acgacctttt
     1381 ccttcatgaa atttaagtat ttcgatatac gcaatatctt ctccatattg atcgaataat
     1441 tgccagccgt ttatcccaac tgatttcaaa tatccctgat tttcgttcaa acgcattaat
     1501 tgctcgaaat cgacaaaaac aactccattt ttcgtccaat aagctatggt atcattcagt
     1561 tttccgacaa ttgtaattcg atcaatcgac caatttaatt ttaggggggt ggtcgtacct
     1621 tgcacactta accgcctgtt agaaatacgg ttaagttttt cgagctgatt tatcgttgtt
     1681 cttttttctg cccctcgacc ttcaattttt gattgaattt cactcaactg attttcattt
     1741 gccattttcg tcctccaaac gaaaaaagag acttttggaa agaatttcca gagttcacgt
     1801 aaccctaaaa attcatatcc aaaaatctct agcttgaaga aacctaaaaa tattcgtata
     1861 atgaacctga gttacggagc taggtgaagt agtttccgta aatagtcgag agtgttagcg
     1921 cactgctcgg cttttatttt gctctttttt ctcgttttct ttgattatag cactcctcct
//
LOCUS       DACCCM010000168         1947 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCCM010000168 DACCCM010000000
VERSION     DACCCM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.178"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            217..1206
                     /locus_tag="IVK78_002860"
     CDS             217..1206
                     /locus_tag="IVK78_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6376243.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1203..1622
                     /locus_tag="IVK78_002861"
     CDS             1203..1622
                     /locus_tag="IVK78_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376244.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1631..1939
                     /locus_tag="IVK78_002862"
     CDS             1631..1939
                     /locus_tag="IVK78_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376245.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
BASE COUNT          694 a          251 c          433 g          569 t
ORIGIN      
        1 aggcaactat ttctgcgaag aaatcggggc tatagtatgg gtctatccct tgtggctcta
       61 aggaagaaca aaaaaaaaga ttgctttttc ttccctagta ttttatagtt ttaattgact
      121 agaaaaaaac taaactagaa aggagagcaa tctatttttt gttatcggct tttcagcctt
      181 ttttgtatac aaaatttgta gattcagtat agcaagatga aaaagaaaaa gcaagatttg
      241 attaacggaa catttcgaga aaaaaagaaa aatttgaaaa aagtggtggc atttagtgat
      301 aatcgaattt ctcccaaaat gcgtgaattg atgacaggct gtggttcata tttagaattt
      361 attgcaacga ttgataagga gaaaaagaaa ttggtccaag ctcacttttg taaaaatcgt
      421 ttctgtccgt tatgtgcttg gcgtaaagct cgtaaggatt cgatgatgtt gtcgattatg
      481 atgcaagcta ttgcggaaga aaagcagtat caatttattt ttatgacgtt gacgacaccg
      541 aatgtaaaag cggaatgttt gaacgatgaa attgatttgt ttaatcatgc attgaaaaag
      601 atgtttatgc gcaaacgggt taagacttcg attaaaggct atgtgcgaaa attggaaatc
      661 acgtacaaca aaaaacggaa tgactataac ccgcattttc acttgatttt ggcagtaaat
      721 aaatcttatt tcacggataa agattattat atcaagcaat cggaatggtt agatttgtgg
      781 cgggaagtga ccggaaaaac gggaattaat ccggatggta ccgacgaaat tacccagttg
      841 gatattcgca aggtcaaagg gttccaacaa gaaaaagcag tacaggaagt tgcgaaatat
      901 tcggcaaaag attttgaact gaccgaaagt caggaagtgt ttgacacatt ctatcatgca
      961 atgaaaggtc gtcagttgat tacgtttaac ggtgtattca aagaatacaa gaaaaaatat
     1021 gagtcgggtg accttgatcg ttataagaaa aaagatgaga atgaatattt ttggtttttg
     1081 actgcttcgt ggatgaggaa agaatcgaaa tatgcggttg attatcgtga gatgagtgct
     1141 gatgaaagaa attatttttc aggaatgtat gtcgatgaaa aatctgttga gggggaaaat
     1201 gaatgactga ttcaacggat aaaatgacaa ttaaggcgct tgctgatgaa ctaggaataa
     1261 ccaaaaatac agtgagaaat tatctagtca aagccggata tgatcttgct cggtacaaga
     1321 aaaatggaat tatcttgcta gacaaagagt tgattggtgt tgctcggtca atttatcggt
     1381 caaaaggtgg tcaattgact ggtcatttac cggtcaatag cacgggcagt gattttcctt
     1441 tggaaaaaga gaatgagttt ttgcgtgatc aattagctga gaaggacaaa catatttctg
     1501 aattaacaga aatgattcgt gatcaacaaa aattgaccct gcttcagcaa caaaaaaacg
     1561 atcagttgtt actagaaaat caagaattaa aaaatcgaaa atggtggcaa ttctggaagt
     1621 gagatgtgac atggataaaa ttgatttgag agaagaaaca gtcaatagaa ttgaagtgat
     1681 gtatgaacgg ttagctgatt atttgaatct ttcggatgaa gataattata gtgagatgct
     1741 tgaacgaatg atggatgttc tcaactcggt tatcaatgct gatctagcaa aatcttcagt
     1801 tttgactaat gaatttgttc ataagcattt agcgcaaaca actgatgaag ctgaaaaaga
     1861 attttggttg aaattaatga gtcatattat gcagttaaac caaatttccg ttattaattc
     1921 caaagaaaag gggatgtagt ttatggg
//
LOCUS       DACCCM010000169         1896 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCCM010000169 DACCCM010000000
VERSION     DACCCM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.179"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(368..1486)
                     /locus_tag="IVK78_002863"
     CDS             complement(368..1486)
                     /locus_tag="IVK78_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376246.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT          556 a          406 c          297 g          637 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctc
//
LOCUS       DACCCM010000170         1718 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCCM010000170 DACCCM010000000
VERSION     DACCCM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.180"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(606..962)
                     /locus_tag="IVK78_002864"
     CDS             complement(606..962)
                     /locus_tag="IVK78_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP6376247.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IVK78_002865"
     CDS             complement(976..1386)
                     /locus_tag="IVK78_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6376248.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          529 a          315 c          242 g          632 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aaggactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcac
//
LOCUS       DACCCM010000171         1698 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCCM010000171 DACCCM010000000
VERSION     DACCCM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.181"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            50..1438
                     /locus_tag="IVK78_002866"
     CDS             50..1438
                     /locus_tag="IVK78_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP6376249.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
BASE COUNT          674 a          203 c          277 g          544 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaa
//
LOCUS       DACCCM010000172         1685 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCCM010000172 DACCCM010000000
VERSION     DACCCM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.182"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..779)
                     /locus_tag="IVK78_002867"
     CDS             complement(45..779)
                     /locus_tag="IVK78_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6376250.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IVK78_002868"
     CDS             1106..1516
                     /locus_tag="IVK78_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP6376251.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..>1685
                     /locus_tag="IVK78_002869"
     CDS             1509..>1685
                     /locus_tag="IVK78_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6376252.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYN"
BASE COUNT          536 a          294 c          325 g          530 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataat
//
LOCUS       DACCCM010000173         1655 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCCM010000173 DACCCM010000000
VERSION     DACCCM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.183"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            200..742
                     /locus_tag="IVK78_002870"
     CDS             200..742
                     /locus_tag="IVK78_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376253.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IVK78_002871"
     CDS             898..1347
                     /locus_tag="IVK78_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP6376254.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1377..>1655)
                     /locus_tag="IVK78_002872"
     CDS             complement(1377..>1655)
                     /locus_tag="IVK78_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6376255.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          624 a          236 c          319 g          476 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attcc
//
LOCUS       DACCCM010000174         1622 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCCM010000174 DACCCM010000000
VERSION     DACCCM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.184"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            216..722
                     /locus_tag="IVK78_002873"
     CDS             216..722
                     /locus_tag="IVK78_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376256.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            709..1161
                     /locus_tag="IVK78_002874"
     CDS             709..1161
                     /locus_tag="IVK78_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376257.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            1154..1552
                     /locus_tag="IVK78_002875"
     CDS             1154..1552
                     /locus_tag="IVK78_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376258.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          644 a          190 c          270 g          518 t
ORIGIN      
        1 cgtaaactag tttggtaata caattgtaat ttattttcag tatttaaaca tttctacctt
       61 attacattat gttaactaaa ttgcttataa aataagctaa tttttattga caagcaggat
      121 gtactatagt taaacttaaa ataagataca aatatatata atatgcttat acatatagta
      181 atatttacta atattgaaac aggtggttta gtgagatgga cattaagtgg ttggttcaac
      241 aaaatgatag caatttagaa ttggcgatta aatatctcga agaaactatc tttgaagatg
      301 aacatcttac agataatttt ttacaagtat taaaatatct tgaaatttac tctgttaaaa
      361 aaaataaatt aattggtgaa aatgattctc caataaaaac acctattgag ctaagcttaa
      421 gaaatcggat gggaatactc caaagatcgg aaatagtaaa agaacttttt tatcataaat
      481 ttagttatga aataagacta gatgatactt atgagcatta tagaattgtt tttttcgtat
      541 acaattctat agaagatgct actgcgacta ctgcattaac attcggtttt acgaaaaatg
      601 gaacaatcaa ttcggataaa acccgtcaag ctgctacaga aagtgatgat atatgtaaga
      661 aagtatgcaa tggggaagag aattactgga taggggagga aaagttgaat gaaatctatt
      721 aatgatttat taaaaaaaca tcctgattta tataaaaaat taaattctga acaaagaaac
      781 ctgtccaaac aattacttaa aataagatta aaaaaacaaa aaaacgtgac agaattctca
      841 aaaatgttag gaataagtga aaaaaaatat cttgaatttg agttcggaga tatgcatatt
      901 cctgttgcag agtacaataa acttttgaaa caagttgaaa atttgtccta taaagattca
      961 atgaatttca ctattaaggt tgctggcggc cctattaaat atgaccgtat tgaaaataaa
     1021 ccttatattc ggaataaaag ttactcagta tttaaaagta gaaagagaaa agaagtatcg
     1081 tcaaatatgg tctatgctag tgttaattac caagatacga gccgctatat tgtagaaaaa
     1141 gaggtatatg tacatggata aaggaaatag tgttactttt gcacttgaaa atattagttt
     1201 gaataaaatt cattttgaat taactgataa ttttagttat ggagatttag agtttaatat
     1261 tgaacttgga tataaagaag acgaagcaga gtatctagaa aaaaaacttg tttctttacg
     1321 agctcacatt gtagggggga tggaaggagc accggtagat attttactct tttcagttat
     1381 tcgtgtttct acaaatgaac agccatataa aaaattagta gaagaatcta tgggagagct
     1441 agctaaacct atcctgaata aagcttctaa tttgatttct aatttatata gcgaagtagg
     1501 agttatgcct acagttattg atttatatca gctatatagt caacaaaaat aaaatacaag
     1561 taatagagtt atctatttaa atttgcaaga caaagacctc aaccagtcat ccttggttga
     1621 gg
//
LOCUS       DACCCM010000175         1615 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCCM010000175 DACCCM010000000
VERSION     DACCCM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.185"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            555..>1615
                     /locus_tag="IVK78_002876"
     CDS             555..>1615
                     /locus_tag="IVK78_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376259.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVY"
BASE COUNT          666 a          259 c          350 g          340 t
ORIGIN      
        1 aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa ttagtacgat
       61 tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga aagaccaacg
      121 gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa gaacgtttag
      181 aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag gtagaaaaac
      241 ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc ctggatatga
      301 caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa ggtgaatcga
      361 ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta gaacagctga
      421 aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca attattgaca
      481 gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa gactatcgaa
      541 aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa taccaagcca
      601 cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg ccaactgatt
      661 ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa aatgatccag
      721 aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc gatcaactca
      781 aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat tttgaagaag
      841 tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc gtcaacagta
      901 atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa aaggaacaaa
      961 aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa gaaacaaaaa
     1021 caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa gtcaaatccc
     1081 tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa gacgtgaaag
     1141 aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa ttattgattg
     1201 aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa aaagaaggaa
     1261 tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg gtcaaaggat
     1321 tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa gataaaaaac
     1381 gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt gaaaaagtgt
     1441 ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt ccgaaccgtg
     1501 tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg attcaagcgg
     1561 tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc tatca
//
LOCUS       DACCCM010000176         1582 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCCM010000176 DACCCM010000000
VERSION     DACCCM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.186"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            290..1477
                     /locus_tag="IVK78_002877"
     CDS             290..1477
                     /locus_tag="IVK78_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6376260.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          316 g          503 t
ORIGIN      
        1 ttaaccttcc ggttttgatt ggataaccct atgaaccatc gttattttaa tttggtatta
       61 actcatttct tttgagataa tatatttatt ggattgagag gctaagccgt tccccccttg
      121 ggccattgaa cccgcactcg agagactggc tcctctctct gataagtgga ttgcgtatga
      181 gttagatgtt gaaagtcctt actttactcc gtatctcgta caaggtagtg acgcttttca
      241 gttgagtgga tttcaatcca aaattctttt tttgaaaggt cagatataca tgtacttttt
      301 aggcattgat atcggtaaac gcactcacgt tgcttctatc atgaacgagg aaggaaaggt
      361 attactaaaa ggtttttcat ttcctaacac cactgaagga gcagagtcat taattgagcg
      421 aatggtcgac tattccggag cacccagtga ctttgttata gggatggaag ccacggggca
      481 ctattggctg tccatctttt cataccttca cgaatcagat tatttgattc acgttgtgaa
      541 tccccttcaa acagacggat ggcgtaaagg tacagaaata cggaaacgaa aaaacgatat
      601 tattgattct gttttgattg ctgacttaat gcgctatggc tcatttgtcg aaacggtctt
      661 atccgatgaa aatgtttttt ctttgaaaca actttctcgt taccgaactt atttagtagg
      721 tacagcgagc gacttcaaaa gaaaaattat agccgtattg gatcaagtct ttccagaata
      781 cgcgacgatt tttaccaagc agggcgtttt cggaaaagct tctaaagagt tattgctggc
      841 gtttagtgcc ccgatcgact tagaaaacat ttcctcagaa actttggccc aatacttagc
      901 tgagttaagc aggaaccgaa tcaaggcaga aaaagcggac caattgaagc aagcagcttc
      961 taactccttt ggagtaaaat ttgctcaaga agcgttcacc ttccaactac gttcaatgat
     1021 tgaacaatta aaattcattg aaagccaaat aaaggaaacg gaaagagaaa tcaagcacat
     1081 aatggacact ttaaattctg ttattctcac aattcctgga attggtccta taaacggtgc
     1141 gactattctt ggtgagatcg gagatattca aaaattttct aacccgaaaa aactcgttgc
     1201 ttacgctggg atagacgctt ccgtgacaca atcagggcaa tatgaggcaa cgcataatgt
     1261 tatgagtaaa cgagggtctc catatttgcg aaaagccttg ttttcggcgg ctttagtagc
     1321 ttctcaatgt gatcctgttt ttagtgcgtt ttaccagaaa aaagtttccg aaggaaaaca
     1381 tcatctaact gccttaggtg cggtttctcg taagctttgt tacgtgattc atgctatttt
     1441 gactaaaaac gagccttata taattgtttc tgattagcta ttgaacattc aaaaaaacta
     1501 atggtaagaa gcttatttgt tgtacccttt tttcggaatt atctgttata aaatattttt
     1561 tgatttggct aatttttact tg
//
LOCUS       DACCCM010000177         1523 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCCM010000177 DACCCM010000000
VERSION     DACCCM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.187"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            80..>1523
                     /locus_tag="IVK78_002878"
     CDS             80..>1523
                     /locus_tag="IVK78_002878"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6376261.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          511 a          302 c          306 g          404 t
ORIGIN      
        1 aaaaaacaag ttcaaatagt aactaatttt ttaaaaaact gatttataaa aaatacaaca
       61 cgaatgagga aaataaccaa tgacagaaca aatcgaaaaa aattcaaaaa gcttatatca
      121 ggcactgtgg aattccgcag atatcctgcg ttcaaaaatg gacgctaatg aatacaagag
      181 ctatttacta ggcttggtct tttataaata cttatccgac aacatgctgc ggtatgtctc
      241 cgttctattg gaggaagaaa cggacgacct aaataaagca caagaccttt acgttgaagc
      301 atttaaagat gcagatataa aggatgatct cttgtatgaa ctaaaagatg agttttcata
      361 taccatcgca cccgcattga cctttaccgc gcaagtggca gccattcatg atggatcttt
      421 tcaattagaa gacctggtac aaggtttccg tgacattgaa caatccagcg aaaactttga
      481 gaacttattt gaagacattg atctgtattc taaaaaactg gggtcaaccc cacaaaaaca
      541 aaataaaacc atcgcagatg ttatgaaaga attagaaggg ttaaacatgg cggggcatgc
      601 tggcgatgtc ttaggcgatg cctatgagta cttaattggc caattcgcat ctgaatccgg
      661 taaaaaagcc ggtgaattct ataccccaca accggtcgca aaattgatga cacaaattgt
      721 tttacaagga aaagaagata aaaaaggctt ttctgtttac gacgcgacca tgggttcggg
      781 ctcgttactt ttgaacgcta aaaaatattc ccatcaaccg ggaaccattt cttatttcgg
      841 acaagagttg aatacgtcta cctataattt ggcgcgtatg aacatgattt tgcatggggt
      901 tcccattgcc aaccaacact tgcataatgc ggataccttg gatcaagact ggccaacaga
      961 agagccaacg aacttcgatg gggtgctcat gaacccaccg tattctgcaa aatggtcggc
     1021 tgataaaggc ttcttagatg acccacgctt ctctgcctat ggcgtattag cccctaaatc
     1081 aaaagcggac tttgctttct tattacatgg ctactatcat ttgaaagata cgggcgtcat
     1141 ggccattgtt ttgccacatg gggtgctctt tagagggggc gcagaaggga agatacgcaa
     1201 agccttgtta gaaaatggtg ccattgacac cgtcattggc ttacctgcta acatcttctt
     1261 taatacgtcc attccaacga ccgtaattat cttgaagaaa gaccgtgcga aaaaagatgt
     1321 gctctttatt gatgcgtcac aagattttga aaaaataaaa acgcaaaata cgctacgaga
     1381 ttaccatatt gacgctattt tagaggccta taaaacccgt acaattaaag acaagtatgc
     1441 ttatgtcgca gaatttgatg aaattgtgga gaatgattac aacttaaaca ttccccgtta
     1501 cgtggatacc tttgaagaag aag
//
LOCUS       DACCCM010000178         1502 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCCM010000178 DACCCM010000000
VERSION     DACCCM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.188"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(312..611)
                     /locus_tag="IVK78_002879"
     CDS             complement(312..611)
                     /locus_tag="IVK78_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376262.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="IVK78_002880"
     CDS             complement(617..847)
                     /locus_tag="IVK78_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376263.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="IVK78_002881"
     CDS             complement(1097..1297)
                     /locus_tag="IVK78_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6376264.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          533 a          243 c          219 g          507 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aa
//
LOCUS       DACCCM010000179         1456 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCCM010000179 DACCCM010000000
VERSION     DACCCM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.189"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            108..1040
                     /locus_tag="IVK78_002882"
     CDS             108..1040
                     /locus_tag="IVK78_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6376265.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IVK78_002883"
     CDS             1112..>1456
                     /locus_tag="IVK78_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376266.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACCCM010000180         1425 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCCM010000180 DACCCM010000000
VERSION     DACCCM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.190"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            100..1392
                     /locus_tag="IVK78_002884"
     CDS             100..1392
                     /locus_tag="IVK78_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6376267.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          258 g          412 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DACCCM010000181         1424 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCCM010000181 DACCCM010000000
VERSION     DACCCM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.191"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(150..1337)
                     /locus_tag="IVK78_002885"
     CDS             complement(150..1337)
                     /locus_tag="IVK78_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP6376268.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCCM010000182         1406 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCCM010000182 DACCCM010000000
VERSION     DACCCM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.192"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(182..255)
                     /locus_tag="IVK78_002886"
     tRNA            complement(182..255)
                     /locus_tag="IVK78_002886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Ile,seq:cat)
     gene            complement(444..1403)
                     /locus_tag="IVK78_002887"
     CDS             complement(444..1403)
                     /locus_tag="IVK78_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6376269.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          416 a          279 c          255 g          456 t
ORIGIN      
        1 taaaaatcaa agtaaaactt ttcaaatata cagaaatata actatgtgaa acatcctttc
       61 attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg aagaaaagct
      121 ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaaat ggctacgtta
      181 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      241 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      301 gtggcaacaa acctgttatc gcatagcttg gagtgtgact attatgggtg atagtgaaga
      361 tatgcgataa catcactatt ttatcgaatg atttttatag ttgtcaatat agttatgtaa
      421 tgctcctcaa cgaggagcta tttttatcgt ttaggaatat ttaaatacca acgtttgtca
      481 tggaaatctt gcgcaccgcc tttagtgttt ccctctggat cattcgttgc ccgcatcatg
      541 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
      601 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      661 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      721 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      781 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      841 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      901 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      961 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
     1021 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
     1081 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
     1141 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
     1201 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
     1261 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
     1321 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
     1381 atagtatagg caaatccgtt aattgg
//
LOCUS       DACCCM010000183         1374 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCCM010000183 DACCCM010000000
VERSION     DACCCM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.193"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            33..1372
                     /locus_tag="IVK78_002888"
     CDS             join(33..474,474..1372)
                     /locus_tag="IVK78_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP6376270.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACCCM010000184         1365 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCCM010000184 DACCCM010000000
VERSION     DACCCM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.194"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            50..1357
                     /locus_tag="IVK78_002889"
     CDS             50..1357
                     /locus_tag="IVK78_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6376271.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT          469 a          267 c          291 g          338 t
ORIGIN      
        1 ataaaatttt aatttgtagc gaaacaaatt aattacaagg agatcggata tgtccataaa
       61 aaaaacacgt ctagtcctat tattggctag tctcattctt tgccttgctt cttttctacc
      121 agctgttgct gtgcaagctg aagatacgtt caaagtaaat gcaaaagcag cttttgctgt
      181 agatgcgcaa agcggtaaga ttctttatga tcaagatgga gaaaaaacca tggggatcgc
      241 ttcaatcaca aaaatcatcg gtctttacat cgtccttgat caagtaaaag aaggaaaact
      301 gtcatggggt gataaagttt cgatttcaga ttatgcagaa aatttgagta tcaccccaga
      361 tttatcgaat gttcctttgc ataaagaaaa cacctacact gtcaaagaat tattcgactc
      421 tgcaatcatc cagtcagcca atgcttcaat ggtggccttg gcagaaaaaa tctctggcag
      481 cgaagctaaa ttcactgaac gaatgaaaga acaattgaaa gattggggaa tcaaagatgc
      541 aacaatcgtt aatgcatcag gattgaacaa ctcttatctt ggcgaaaacc gaccagaagg
      601 cactggagaa aacgacgaaa atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca
      661 tttgatcttg gatttcccag aaatactgga tgtatcaagt actacgaccc aaatgtttgg
      721 cgaaaatacg caatcaccag tggaaatggt caactggaac tggatgctcc caggattcgt
      781 taattacaag gaaggcgtgg acggattaaa aactggtacg actgagcttg caggtgcttg
      841 tttcgttggt acgatcacta aaaacgatca acggatcatc actgtcgtat tgaatgcgga
      901 cggccatgcg gaaaacccga gtgcccgctt caatgaaaca gaccgtttga tggactactg
      961 ctatgacaat tggtcagaaa aagagctcgg aaaagctaac gcaagtattc ctagtctaaa
     1021 aaccattgat gtaaaagacg gaaaagaatc aacagttcct gttgtgctta aaagccctgt
     1081 aaaagtatgg gtacgaaacg atatggatac tggcaaatta acgatcacgc caacacttga
     1141 caagaagcaa gtcactgaca atgagatcca agcacctatg gataaaggta caaaaatcgg
     1201 taccgctacg atcaccttgg cagacgataa attaggctat ttagaagaca acaatgcacc
     1261 atcaacagag atcattacgg ataaaacagt ggaaaaagcg aatatattcg tgattggctg
     1321 gcgacgcgtc gcagaatttt tcggcaactt gttttaattg aacaa
//
LOCUS       DACCCM010000185         1360 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCCM010000185 DACCCM010000000
VERSION     DACCCM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.195"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            25..1326
                     /locus_tag="IVK78_002890"
     CDS             25..1326
                     /locus_tag="IVK78_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6376272.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          442 a          271 c          252 g          395 t
ORIGIN      
        1 aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact actacttgga
       61 atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa gaatatcaag
      121 agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt gtgtaaacag
      181 atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat tcaacttaat
      241 aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca ctgtcgtggc
      301 tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt tatcgctaaa
      361 caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac gacaattgct
      421 aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct gatccccatg
      481 acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt tcgttcactg
      541 aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg aaaattactg
      601 gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca atttgaacga
      661 cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta tgcctctctg
      721 ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat tgtcaaacat
      781 ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga tcgaaacgat
      841 ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct gatcaaacga
      901 caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg ggcgtatctc
      961 accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg aatcgcctat
     1021 ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac tttctttaaa
     1081 cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa aaaagctttt
     1141 ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa tgccaaaata
     1201 gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt tcgaagcttt
     1261 cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga atcaaaaaac
     1321 atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DACCCM010000186         1351 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCCM010000186 DACCCM010000000
VERSION     DACCCM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.196"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1351
                     /locus_tag="IVK78_002891"
     rRNA            <1..>1351
                     /locus_tag="IVK78_002891"
                     /product="23S ribosomal RNA"
BASE COUNT          360 a          294 c          413 g          284 t
ORIGIN      
        1 gcagtaagtc tgaagaggag tcaaatgctt cttttcttag ggacaagctg tgatggggag
       61 ggaaataata gtaccgaagt tcctgatgtc acactgccga gaaaagcttc tagtgagaaa
      121 acagcggccc gtaccgcaaa ccgacacagg tagtcgagga gagaatccta aggtgagcga
      181 gagaactctc gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga aggggtgctg
      241 atcatacgat cagccgcagt gaataggccc aagcgactgt ttatcaaaaa cacaggtctc
      301 tgcaaaatcg taagatgaag tataggggct gacgcctgcc cggtgctgga aggttaagag
      361 gagtgcttag cgcaagcgaa ggtacgaatt gaagccccag taaacggcgg ccgtaactat
      421 aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac gaaaggcgta
      481 acgatttggg cactgtctca acgagagact cggtgaaatt ttagtacctg tgaagatgca
      541 ggttacccgc gacaggacgg aaagacccca tggagcttta ctgtagtttg atattgagtg
      601 tctgtaccgc atgtacagga taggtaggag ccgtagaaat cggaacgcta gtttcgatgg
      661 aggcgctggt gggatactac ccctgcgtta tggccactct aacccgcacc actaatcgtg
      721 gtgggagaca gtgtcagatg ggcagtttga ctggggcggt cgcctcctaa aaggtaacgg
      781 aggcgcccaa aggttccctc agaatggttg gaaatcattc gaagagtgta aaggcagaag
      841 ggagcttgac tgcgagacca acaagtcgag cagggacgaa agtcgggctt agtgatccgg
      901 tggttccgca tggaagggcc atcgctcaac ggataaaagc taccctgggg ataacaggct
      961 tatctccccc aagagtccac atcgacgggg aggtttggca cctcgatgtc ggctcgtcgc
     1021 atcctggggc tgtagtcggt cccaagggtt gggctgttcg cccattaaag cggcacgcga
     1081 gctgggttca gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg ttggaaattt
     1141 gagaggagct gtccttagta cgagaggacc gggatggact taccgctggt gtaccagttg
     1201 ttctgccaag ggcattgctg ggtagctatg tagggaaggg ataaacgctg aaagcatcta
     1261 agtgtgaagc ccacctcaag atgagatttc ccatttcttt aagaaagtaa gatccctgag
     1321 agatgatcag gtagataggt caggagtgga a
//
LOCUS       DACCCM010000187         1308 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCCM010000187 DACCCM010000000
VERSION     DACCCM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.197"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            241..314
                     /locus_tag="IVK78_002892"
     tRNA            241..314
                     /locus_tag="IVK78_002892"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:275..277,aa:Ile,seq:cat)
     gene            694..855
                     /locus_tag="IVK78_002893"
                     /pseudo
     CDS             694..855
                     /locus_tag="IVK78_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            1005..1205
                     /locus_tag="IVK78_002894"
     CDS             1005..1205
                     /locus_tag="IVK78_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6376273.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT          419 a          207 c          267 g          415 t
ORIGIN      
        1 ttttttgctc tgtaattaac tcgtatagat cctgcaagat agtttgtttc tgaataaatg
       61 attgatatga agtatttaga tgactgtatt gacaactata aaaatcattc gataaaatag
      121 tgatgttatc gcatatcttc actatcaccc ataatagtca cactccaagc tatgcgataa
      181 caggattgtt gccacacctt ctactggttg attgtttatg gctttatgtg gcaacaacct
      241 gtactcttag ctcagttggt cagagcagac ggctcataac cgtccggtcg taggttcgag
      301 tcctacaggg tacataattt aatctatttg ataaaaaata aaattcaata atggtataat
      361 aaaatgtcgg ctcgtcgctt ccacagagat tttcgtggga ggaggtgtat gatttgtttg
      421 aattcacttt ggagcacgtg ctcttgccat tgttaattgg tttaactttg gcattattta
      481 gtcattggct agataaacat tagagccgac aatggcgctt actgtttcgc agcggtaagc
      541 gtcttttttt gaaaaagaaa aaaacacaac gtattcgcag gtacgttgtg tttatggtgt
      601 atatttgttt aaattcactt tcaattatta ttatacttgt taaataaatt gtgtcaatat
      661 aaaagatatt tttatacgtg tactaaccta tattatcctt ctggtaatgt tattccatcg
      721 cagcaggata tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact
      781 gtactggacc acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg
      841 atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg
      901 taattttgtt tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat
      961 gttacaattt aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag
     1021 taaaatggtt caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg
     1081 tattcgttca cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag
     1141 cagtgacttt cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag
     1201 cttaatttta tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc
     1261 gagtaacaaa aatacctgcc acaagagaca atttctcttg tggcaggt
//
LOCUS       DACCCM010000188         1294 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCCM010000188 DACCCM010000000
VERSION     DACCCM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.198"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1244
                     /locus_tag="IVK78_002895"
     CDS             <1..1244
                     /locus_tag="IVK78_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6376274.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACCCM010000189         1288 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCCM010000189 DACCCM010000000
VERSION     DACCCM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.199"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            282..521
                     /locus_tag="IVK78_002896"
     CDS             282..521
                     /locus_tag="IVK78_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376275.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IVK78_002897"
     CDS             complement(883..1143)
                     /locus_tag="IVK78_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376276.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          426 a          235 c          175 g          452 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctcttttt
//
LOCUS       DACCCM010000190         1282 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCCM010000190 DACCCM010000000
VERSION     DACCCM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.200"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(78..1067)
                     /locus_tag="IVK78_002898"
     CDS             complement(78..1067)
                     /locus_tag="IVK78_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6376277.1"
                     /translation="MTDKKQDLINGTFKMKKKNLQKVIDFGGGRFSERTVERMASCGS
                     YLEFIATADKEKKKLIQANFCTNRFCPLCAWRKARKDSLMLSIMMQAIAEEKNYQFIF
                     MTLTTPNVKADKLNEEIDLFNHALSKMFRRKRVMGAIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDPKYYIKQAEWLDLWRDVTGKTGINLDGTDEISQLDIRKVKGFQQ
                     DKAVQELAKYSAKDFEMTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYEAGELDHY
                     KKKDENEYFWFLTASWMKKESSYAINLRELTPDERKFFSGGLVEEKPVEGENE"
BASE COUNT          353 a          298 c          198 g          433 t
ORIGIN      
        1 cccataaact acatcccctt ttccttggaa ttaataacag aaatttggtt taatcgttat
       61 tttatcaggt gaatcagtca ttcattttcc ccctcaacag gtttttcctc aactaaccca
      121 ccgctaaaaa atttcctctc atcaggcgtc aattctcgga gatttattgc atacgacgat
      181 tcctttttca tccatgaagc agtcaaaaac cagaaatatt cgttctcgtc ttttttcttg
      241 tagtgatcga gctcgcctgc ttcgtatttc ttcttgtact ccttgaagac cccgttaaac
      301 gtgattaact ggcgcccttt catagcatgg taaaacgtat caaagacttc ttggctctcg
      361 gtcatttcaa agtctttcgc agaatacttg gcaagttcct gtaccgcttt gtcttgttgg
      421 aatcctttga cctttcgaat atccaactga ctgatttcgt ctgtaccatc aagattgatt
      481 cccgttttcc cagtcacatc acgccacaaa tcaagccatt ctgcctgttt aatataatat
      541 ttagggtcag taaaatacga cttgttcacc gccaaaatca aatgaaaatg cgggttgtag
      601 tcatttcgtt tcttgttgta cgtgatttcc aatttccgga catagccttt aatcgcaccc
      661 atgacccttt tcctacgaaa cattttggac aacgcatgat taaataaatc aatttcttcg
      721 tttagcttgt ctgcttttac attgggcgtt gttagcgtca taaaaataaa ctggtaattc
      781 ttttcttcag cgattgcctg catcataatc gacaacatca acgaatcttt acgggctttg
      841 cgccatgcac acagcggaca aaagcgattc gtgcaaaaat tcgcctgaat caatttcttt
      901 ttctccttgt cagcggtggc gataaattcc aagtatgaac cacagctagc catacgctcc
      961 actgtccttt ccgaaaagcg cccgcccccg aaatcaatca ccttttgtaa atttttcttt
     1021 ttcattttga atgtcccatt tattaaatct tgttttttgt ctgtcatatt gatatactga
     1081 acctacaatt ttgtataaaa aggctgaaaa gccgataaca aaaaaaatag attgctctcc
     1141 ttaatttggt tggtttttac ttagacacta aaagcttaat ccaatatgct aggaaaagca
     1201 atcttttttt tgtgcaaccc cttatgtatc aaggtcaaaa gcatactata cgcaactatt
     1261 tcctgccgaa aataattgcg ta
//
LOCUS       DACCCM010000191         1276 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCCM010000191 DACCCM010000000
VERSION     DACCCM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.201"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            35..532
                     /locus_tag="IVK78_002899"
     CDS             35..532
                     /locus_tag="IVK78_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6376278.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IVK78_002900"
     CDS             complement(569..1252)
                     /locus_tag="IVK78_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376279.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          217 c          224 g          391 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACCCM010000192         1215 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCCM010000192 DACCCM010000000
VERSION     DACCCM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.202"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            414..947
                     /locus_tag="IVK78_002901"
     CDS             414..947
                     /locus_tag="IVK78_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP6376280.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACCCM010000193         1197 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCCM010000193 DACCCM010000000
VERSION     DACCCM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.203"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..153
                     /locus_tag="IVK78_002902"
     CDS             <1..153
                     /locus_tag="IVK78_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6376281.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IVK78_002903"
     CDS             159..575
                     /locus_tag="IVK78_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376282.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IVK78_002904"
     CDS             681..944
                     /locus_tag="IVK78_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6376283.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1197
                     /locus_tag="IVK78_002905"
     CDS             944..>1197
                     /locus_tag="IVK78_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6376284.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          455 a          153 c          243 g          346 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggac
//
LOCUS       DACCCM010000194         1196 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCCM010000194 DACCCM010000000
VERSION     DACCCM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.204"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            16..1173
                     /locus_tag="IVK78_002906"
                     /pseudo
     CDS             16..1173
                     /locus_tag="IVK78_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          399 a          238 c          234 g          325 t
ORIGIN      
        1 gattttacta cagaaattct aaagactcta gcgaacaaag gcgatttgaa tgaattattc
       61 cgtgtccatt tggaaaaagc tgtcaatacg cttctcaaaa cggagttaac ggctttcctc
      121 gattacgaaa agtacgatcg cattggtttt aacacgggga attctcgtaa cggctcctat
      181 gaccgtacgg tcaagaccga gtacggggaa cttcatctcc agattccgcg cgaccgcaac
      241 ggcgagttca agcaacagac tgttcctgct tatagacgga cgaatgatac cttagaggag
      301 accgtcattc acctcttccg aaaaggtatt accatgtcgg aaatcgcaga cttgattgag
      361 aaaatgtatg ggcatcacta cacgccccaa accatgtcca acatgactaa agtgctgact
      421 gaagaagtaa atgcatttaa agttagaact ctaaatgata agtatgtcgc tattttcatg
      481 gatgctactt atattccttt aaaacggcaa accgtctcca aagaagcaat ctatattgcc
      541 attggtatac gagaagacgg cactaaagaa gtactaagtt atgcgattgc tccgactgaa
      601 tcgacctacg tttggaatga gttgttacag gacatttact ccagaggggt tcatgatgtc
      661 ttactgttta ttaccgatgg cttaaaaggc atgaaagata cgattcacca gatttatcct
      721 aaagctaaat accaacattg ctgtgttcac gtttcccgta atattgctca taaagtacgt
      781 gtcaaagatc gaaaagaaat ctgtgatgat tttaaggctg tttatcaagc tagctcaaag
      841 gaagaggcaa ataccttttt agggagtatg attgagaagt agcaaaaaac ttatcctaaa
      901 gtgacgcagt cactgataaa aaatcaagat ttattgactt tttatgagtt tccgcctggt
      961 atccgccgaa gcatttactc aactaattta atcgaatctt tcaagaaaca aatcaagaaa
     1021 tacagccgca gaaaagagca gtttcaaaat gaagaatcac tagagcgttt cctagtatcc
     1081 atctttgata catacaatca aaaattctta aatagaagcc ataaaggctt ccaacaagtg
     1141 acggatacat tagcttcaat gtttactgag taacccatta ttttgcagaa ggacga
//
LOCUS       DACCCM010000195         1193 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCCM010000195 DACCCM010000000
VERSION     DACCCM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.205"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1076
                     /locus_tag="IVK78_002907"
     CDS             <1..1076
                     /locus_tag="IVK78_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6376285.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACCCM010000196         1188 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCCM010000196 DACCCM010000000
VERSION     DACCCM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.206"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            141..398
                     /locus_tag="IVK78_002908"
     CDS             141..398
                     /locus_tag="IVK78_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376286.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IVK78_002909"
     CDS             complement(630..1043)
                     /locus_tag="IVK78_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP6376287.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DACCCM010000197         1091 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCCM010000197 DACCCM010000000
VERSION     DACCCM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.207"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            6..980
                     /locus_tag="IVK78_002910"
     CDS             6..980
                     /locus_tag="IVK78_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6376288.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACCCM010000198         1069 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCCM010000198 DACCCM010000000
VERSION     DACCCM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.208"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..>1069
                     /locus_tag="IVK78_002911"
     CDS             82..>1069
                     /locus_tag="IVK78_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP6376289.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPP"
BASE COUNT          361 a          204 c          207 g          297 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccc
//
LOCUS       DACCCM010000199         1058 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCCM010000199 DACCCM010000000
VERSION     DACCCM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.209"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(36..992)
                     /locus_tag="IVK78_002912"
     CDS             complement(36..992)
                     /locus_tag="IVK78_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376290.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACCCM010000200         1006 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCCM010000200 DACCCM010000000
VERSION     DACCCM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.210"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(125..772)
                     /locus_tag="IVK78_002913"
     CDS             complement(125..772)
                     /locus_tag="IVK78_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP6376291.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT          281 a          193 c          166 g          366 t
ORIGIN      
        1 aaggggctgt ccccagactt tagtaggcgt cattagctat ctttcgttag catttaaagg
       61 cgaacaccat cacctattca cttccattaa aagatagcat atttttctgt aaaaaacaat
      121 aaaattatcg ttctttcttt acacgttcag caaattcata gaaaggagcc aatttcgtta
      181 actgttgatt acattggtca ctatcatgac aaatataatt cccctttttc gtatacgtgc
      241 catcagaacc tgctttcgtt gtagaaagaa aaagcgaaac attcgatgtt ttctgacaga
      301 ttgcacagat tcccttattt atcgttggtg ataaagtacc gctaatccct tttaattttt
      361 catcttcgta atagaccatg aattttttct gcgtacctgg atcattccag ccgatgtacg
      421 tatgttcttg caagtcgatt tcattccaag ctggttgttt gagttttttc acttttcgga
      481 ataatttctc gatttgtggt ttgctaggct gcataaacgg ctgaacgatt tgcttcagct
      541 cctccaatgc tctttctgct tgcgcttttg tcagttttac gtctaaaact gtcgttaaaa
      601 atgtatcgat ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcctgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaa
//
LOCUS       DACCCM010000201         1002 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCCM010000201 DACCCM010000000
VERSION     DACCCM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.211"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            52..307
                     /locus_tag="IVK78_002914"
                     /pseudo
     CDS             52..307
                     /locus_tag="IVK78_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            371..1002
                     /locus_tag="IVK78_002915"
                     /pseudo
     CDS             371..1002
                     /locus_tag="IVK78_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          344 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt ac
//
LOCUS       DACCCM010000202          984 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCCM010000202 DACCCM010000000
VERSION     DACCCM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.212"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            94..969
                     /locus_tag="IVK78_002916"
     CDS             94..969
                     /locus_tag="IVK78_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6376292.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSDDKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPQR"
BASE COUNT          343 a          167 c          199 g          275 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa ctacttcaga tgataaacca gtcattgatc caactagagc aggtgcagca
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg agaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaatt ataaatacga gtatattttc
      721 attatggatt acaatactgg aaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa atcaagctta taatacttct ggaaacgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctaataa gaaagtctat gtaatgatgc gtgcaacgaa tgatccagaa
      901 gggaacacta aaggcggagc acaagatttt catgataaac gctggtattt aaatattccg
      961 caacgataaa aaaagcccct cgat
//
LOCUS       DACCCM010000203          943 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCCM010000203 DACCCM010000000
VERSION     DACCCM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.213"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            2..>943
                     /locus_tag="IVK78_002917"
     rRNA            2..>943
                     /locus_tag="IVK78_002917"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACCCM010000204          891 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCCM010000204 DACCCM010000000
VERSION     DACCCM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.214"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            10..>891
                     /locus_tag="IVK78_002918"
     CDS             10..>891
                     /locus_tag="IVK78_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6376293.1"
                     /translation="MTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMS
                     IRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYT
                     RRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTK
                     ELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLE"
BASE COUNT          317 a          146 c          198 g          230 t
ORIGIN      
        1 atcttaaaag tgacccgatc actgaatctt tttacgactg aaggaacacg aagtcaacgg
       61 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgattgct
      121 acagtaggtg tcggagataa tccccttttg gctaaactag ccttagacaa cgaagcaaag
      181 cataatgaag gatttgttgc ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat
      241 attcctgaaa tgacggactt ttggggaatt ggctctcgaa tgaaaaaaag actcaatcag
      301 atggggatta tgagcattag agatcttgct aactggaatc cttatacgat taagaataga
      361 ctaggtgtga ttggcttaca gctgtacttc catgctaatg ggatcgatcg aacagatata
      421 gcaatcccac cagaaccgac aaaggaaaaa tcatatggaa attcacaagt gttaccacga
      481 gattatacta gaagaaatga aatcgagctg gtggtaaaag aaatggcgga acaagtagcg
      541 atacgcatta ggcagcataa ctgtaaaaca ggttgtgtcc atttacacat tggcacgtcg
      601 atcttagaaa cgagaccagg gttttcacac cagatgaaga tccctatcac agataataca
      661 aaggaattgc agaattattg tcttttcttg tttgataagt attatgaagg acaagaagta
      721 aggcatgttg gaataaccta tagcaagctt gtgtacaccg attcgcttca gctggattta
      781 tttagtgatc cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt
      841 cggcaaaaat atggttttac gtcgattgta catgcttctt caatgttaga a
//
LOCUS       DACCCM010000205          891 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCCM010000205 DACCCM010000000
VERSION     DACCCM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.215"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            10..>891
                     /locus_tag="IVK78_002919"
     CDS             10..>891
                     /locus_tag="IVK78_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6376294.1"
                     /translation="MTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGILS
                     IRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYT
                     RRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKIPITDNTK
                     ELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEENLDKIIDK
                     IRQKYGFTSIVHASSMLE"
BASE COUNT          321 a          142 c          192 g          236 t
ORIGIN      
        1 attttaaaag tgacaaaatc actgaatctt tttacgactg aagaaacacg aagtcagcga
       61 aggaagaagc tcgctcaaat gatccaagaa cgtattaaag aagagttagg tttgattgct
      121 acggtaggcg tcggcgataa tccccttttg gctaaattag ccttagacaa tgaagcaaag
      181 cataatgaag gatttattgc ggaatggacg tacgaaaacg ttcctgagaa agtatggaat
      241 attcccgaaa tgacggactt ttggggaatt ggttcgcgaa tgaaaaaaag gctcaaccaa
      301 atgggaattt tgagcattag agaccttgct aactggaatc cttatacgat taagaataga
      361 ttaggtgtga ttggattaca gctgtacttc catgctaacg gaatcgatcg aacagatata
      421 gcaatcccgc cagaaccgac aaaggaaaaa tcttatggta attcacaagt gttaccacga
      481 gattatacta gaagaaatga aatcgagctg gtggtaaaag aaatggcgga acaagtagcc
      541 attcgtatca ggcagcataa ttgtaaaaca ggatgtgttc atttaaacat tggtacgtcg
      601 atcttagaaa cgagaccagg attttcacac cagatgaaaa tacctatcac agataataca
      661 aaggaattgc agaattattg tcttttcttg tttgataagt attatgaagg acaagaagta
      721 aggcacgttg gaataaccta tagcaagctt gtgtacaccg attcgcttca gctggattta
      781 tttagtgatc cacaaaagca aattaacgaa gaaaatttag ataaaattat tgacaagatt
      841 cggcaaaaat atggttttac gtcgattgta catgcttctt caatgttaga a
//
LOCUS       DACCCM010000206          808 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCCM010000206 DACCCM010000000
VERSION     DACCCM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.216"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            15..>808
                     /locus_tag="IVK78_002920"
     CDS             15..>808
                     /locus_tag="IVK78_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6376295.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVK"
BASE COUNT          230 a          158 c          162 g          258 t
ORIGIN      
        1 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
       61 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
      121 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
      181 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
      241 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
      301 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
      361 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
      421 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
      481 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
      541 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
      601 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
      661 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
      721 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
      781 gaactttctt gaatcatcga gtgaaaga
//
LOCUS       DACCCM010000207          806 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCCM010000207 DACCCM010000000
VERSION     DACCCM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.217"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            74..403
                     /locus_tag="IVK78_002921"
     CDS             74..403
                     /locus_tag="IVK78_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6376296.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..680
                     /locus_tag="IVK78_002922"
     CDS             393..680
                     /locus_tag="IVK78_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6376297.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYDQLKKLI"
BASE COUNT          298 a           96 c          152 g          260 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtggaac gcatgaaaac ttttatgatc
      661 agttgaaaaa attaatctag tgatttgttg tcgttcttaa gaaggacggc ttctttttta
      721 tcgaaaatct gattcgtcta catgactaaa gtcgcgagta ttctctgctt ctcgataaat
      781 taaataacgt cattactttg aggaaa
//
LOCUS       DACCCM010000208          793 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCCM010000208 DACCCM010000000
VERSION     DACCCM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.218"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            27..>793
                     /locus_tag="IVK78_002923"
     CDS             27..>793
                     /locus_tag="IVK78_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP6376298.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT          284 a          148 c          136 g          225 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc
      541 aaaccgtatc caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag
      601 aagtactgag ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac
      661 aggatattaa ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag
      721 gcatgaaaga tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc
      781 atgtatctcg taa
//
LOCUS       DACCCM010000209          790 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCCM010000209 DACCCM010000000
VERSION     DACCCM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.219"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..782
                     /locus_tag="IVK78_002924"
     CDS             <1..782
                     /locus_tag="IVK78_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6376299.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc
//
LOCUS       DACCCM010000210          775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCCM010000210 DACCCM010000000
VERSION     DACCCM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.220"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(249..773)
                     /locus_tag="IVK78_002925"
     CDS             complement(249..773)
                     /locus_tag="IVK78_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376300.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DACCCM010000211          753 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCCM010000211 DACCCM010000000
VERSION     DACCCM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.221"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            363..731
                     /locus_tag="IVK78_002926"
     CDS             363..731
                     /locus_tag="IVK78_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6376301.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          244 a          117 c          157 g          235 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt ttt
//
LOCUS       DACCCM010000212          734 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCCM010000212 DACCCM010000000
VERSION     DACCCM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.222"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(52..240)
                     /locus_tag="IVK78_002927"
                     /pseudo
     CDS             complement(52..240)
                     /locus_tag="IVK78_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            220..>734
                     /locus_tag="IVK78_002928"
     CDS             220..>734
                     /locus_tag="IVK78_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6376302.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT          255 a          114 c          162 g          203 t
ORIGIN      
        1 ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt
       61 cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat
      121 ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag
      181 gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta
      241 acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag
      301 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
      361 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg
      421 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
      481 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
      541 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
      601 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
      661 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
      721 agcagaatga taga
//
LOCUS       DACCCM010000213          718 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCCM010000213 DACCCM010000000
VERSION     DACCCM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.223"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            312..608
                     /locus_tag="IVK78_002929"
     CDS             312..608
                     /locus_tag="IVK78_002929"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6376303.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..718)
                     /locus_tag="IVK78_002930"
                     /pseudo
     CDS             complement(531..718)
                     /locus_tag="IVK78_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          244 a          134 c          105 g          235 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatat
//
LOCUS       DACCCM010000214          694 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCCM010000214 DACCCM010000000
VERSION     DACCCM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.224"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          247 a          116 c           84 g          247 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta atggaatggc aaca
//
LOCUS       DACCCM010000215          683 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCCM010000215 DACCCM010000000
VERSION     DACCCM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.225"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..397
                     /locus_tag="IVK78_002931"
     CDS             <1..397
                     /locus_tag="IVK78_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376304.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            397..>683
                     /locus_tag="IVK78_002932"
     CDS             397..>683
                     /locus_tag="IVK78_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376305.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNG"
BASE COUNT          240 a          109 c          160 g          174 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
      361 agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
      421 gaagacggag ttgctaaata cttaaaaacg catgtagaag ctattgatgg taaagaagca
      481 ttggtgcaga cagatggaga ccaagccatt gcaggacata agaatttttc aggttctgta
      541 acaatcaata ataaacgtgt tttgacgact gatgataaca gatatgaagt ggtaaatctg
      601 gttgtcacta acggtaatac agggacagca aagctttatc gtgaaggaaa aacagtttct
      661 atatattttg tggctttaaa cgg
//
LOCUS       DACCCM010000216          668 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCCM010000216 DACCCM010000000
VERSION     DACCCM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.226"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            179..610
                     /locus_tag="IVK78_002933"
     CDS             179..610
                     /locus_tag="IVK78_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376306.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          260 a          100 c          135 g          173 t
ORIGIN      
        1 gaaggctatc ggacaccaat tagttttgaa caactggttg ggtcaataga ccgttctact
       61 ttgaacagtg ctcaactatc tattggcgca gacggagcca ttaaatggag aagaaattca
      121 agttatggat cagcttattc atttgttatc acttattcaa tttaagggag gaaatctaat
      181 gaaagtagtt tacaaatcaa tcaagcctta cggattcgag caaatcattt tgaacaatca
      241 agaaaatatc cctgaaaact gtacagagat taaaccacca gttcctaact ggagaccaag
      301 atttgatttt gataaaaaac agtgggttga gttagctact gaagaagaga aaaaagggaa
      361 tgctgttgat agcgtagatg aattggctaa tattaaggca ttatacgaaa cgctgaaggc
      421 agaaaatgat gaattgaaac agctaaattc taaagcaatg ctaaacaatg tagcaatcaa
      481 gcaagaaaat gtcttattga aagaaaagtc agaaagttta gcgcagttga attcaaaaac
      541 gatgcttgct tctgtacaaa ataccaagga aatcgaagaa atcaaaaaac aattacaagg
      601 tgggaagtaa catgtattca tatgaagata tcaaactaat gtatgactgg ggctgtttca
      661 ctaacgaa
//
LOCUS       DACCCM010000217          650 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCCM010000217 DACCCM010000000
VERSION     DACCCM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.227"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>650
                     /locus_tag="IVK78_002934"
     CDS             <1..>650
                     /locus_tag="IVK78_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6376307.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          112 c          134 g          191 t
ORIGIN      
        1 caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca
       61 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
      121 tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga
      181 cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac
      241 tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg
      301 tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt
      361 tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat
      421 tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca aatccatcac
      481 ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca atgatattgc
      541 catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg
      601 attgttacgt agagatggtt tattgaaatc tatggatttc aattcagtag
//
LOCUS       DACCCM010000218          641 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCCM010000218 DACCCM010000000
VERSION     DACCCM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.228"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>641)
                     /locus_tag="IVK78_002935"
     rRNA            complement(<1..>641)
                     /locus_tag="IVK78_002935"
                     /product="16S ribosomal RNA"
BASE COUNT          129 a          212 c          134 g          166 t
ORIGIN      
        1 accctccaac acttagcact catcgtttac ggcgtggact accagggtat ctaatcctgt
       61 ttgctcccca cgctttcgag cctcagcgtc agttacagac cagagagccg ccttcgccac
      121 tggtgttcct ccatatatct acgcatttca ccgctacaca tggaattcca ctctcctctt
      181 ctgcactcaa gtctcccagt ttccaatgac cctccccggt tgagccgggg gctttcacat
      241 cagacttaag aaaccgcctg cgctcgcttt acgcccaata aatccggaca acgcttgcca
      301 cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttctggt tagataccgt
      361 caagggatga acagttactc tcatccttgt tcttctctaa caacagagtt ttacgatccg
      421 aaaaccttct tcactcacgc ggcgttgctc ggtcagactt tcgtccattg ccgaagattc
      481 cctactgctg cctcccgtag gagtttgggc cgtgtctcag tcccaatgtg gccgatcacc
      541 ctctcaggtc ggctatgcat cgtggccttg gtgagccgtt acctcaccaa ctagctaatg
      601 caccgcgggt ccatccatca gcgacacccg aaagcgcctt t
//
LOCUS       DACCCM010000219          598 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCCM010000219 DACCCM010000000
VERSION     DACCCM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.229"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(310..>598)
                     /locus_tag="IVK78_002936"
     CDS             complement(310..>598)
                     /locus_tag="IVK78_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376308.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          207 a           98 c           90 g          203 t
ORIGIN      
        1 attgtgagat gataataaag aagagtttaa agcgcacccc aagccacttc cccataagtg
       61 tgttacgctt taaactcttt tatatttgaa gctattaaaa ggcataccat atttttgaaa
      121 aaaagtgaga aaaaaggctt acaattggag tggtagttaa ttagtgactt atttttgatt
      181 ttatagcact gatactataa aatatagata tcatcatatt acacaatctt aatactaact
      241 taaaaaatat ctcctttcac aagtatggtg ataaaattcg ttccgggcta cctttttagg
      301 tagcctactt taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata
      361 acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca aaaagtatac
      421 atccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat tagaatagta
      481 gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat tccatacttt
      541 ataaaaatct ttgataaatc tattcgaagg gtcaaattta aaccataatt gtgtctca
//
LOCUS       DACCCM010000220          593 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCCM010000220 DACCCM010000000
VERSION     DACCCM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.230"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(318..581)
                     /locus_tag="IVK78_002937"
     CDS             complement(318..581)
                     /locus_tag="IVK78_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP6376309.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACCCM010000221          577 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCCM010000221 DACCCM010000000
VERSION     DACCCM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.231"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVK78_002938"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVK78_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP6376310.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          183 a          123 c           93 g          178 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttg
//
LOCUS       DACCCM010000222          509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCCM010000222 DACCCM010000000
VERSION     DACCCM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.232"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          228 a           70 c           81 g          130 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DACCCM010000223          509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCCM010000223 DACCCM010000000
VERSION     DACCCM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.233"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..132)
                     /locus_tag="IVK78_002939"
     CDS             complement(<1..132)
                     /locus_tag="IVK78_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6376311.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACCCM010000224          488 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCCM010000224 DACCCM010000000
VERSION     DACCCM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.234"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..414)
                     /locus_tag="IVK78_002940"
     CDS             complement(<1..414)
                     /locus_tag="IVK78_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376312.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DACCCM010000225          487 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCCM010000225 DACCCM010000000
VERSION     DACCCM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.235"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>487)
                     /locus_tag="IVK78_002941"
     CDS             complement(<1..>487)
                     /locus_tag="IVK78_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP6376313.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACCCM010000226          481 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCCM010000226 DACCCM010000000
VERSION     DACCCM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.236"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..453
                     /locus_tag="IVK78_002942"
     CDS             <1..453
                     /locus_tag="IVK78_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6376314.1"
                     /translation="KKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          172 a           95 c           84 g          130 t
ORIGIN      
        1 aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt aaactatgaa
       61 aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat tgccgatcga
      121 ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga cattctttat
      181 gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga cattagcggt
      241 ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc acagatccaa
      301 aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa ccacatcaaa
      361 gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact acgaattatg
      421 attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagaggt tttcttcttt
      481 c
//
LOCUS       DACCCM010000227          467 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCCM010000227 DACCCM010000000
VERSION     DACCCM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.237"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(77..166)
                     /locus_tag="IVK78_002943"
     tRNA            complement(77..166)
                     /locus_tag="IVK78_002943"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(128..130),aa:Ser,seq:tga)
     gene            complement(190..263)
                     /locus_tag="IVK78_002944"
     tRNA            complement(190..263)
                     /locus_tag="IVK78_002944"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Ile,seq:cat)
     gene            complement(277..350)
                     /locus_tag="IVK78_002945"
     tRNA            complement(277..350)
                     /locus_tag="IVK78_002945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Met,seq:cat)
     gene            complement(378..450)
                     /locus_tag="IVK78_002946"
     tRNA            complement(378..450)
                     /locus_tag="IVK78_002946"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(415..417),aa:Ala,seq:tgc)
BASE COUNT          124 a          130 c          113 g          100 t
ORIGIN      
        1 aggatttgaa cctacgacct tcgggttatg agcccgacga gctaccagac tgctccatcc
       61 cgcgataata tgaaaaagga ggataaggga ttcgaaccct tgcacggttt tacccgcctg
      121 acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccatat gaaacttaca
      181 acgccacaat ggaccttgta ggactcgaac ctacgaccgg acggttatga gccgtctgct
      241 ctaaccaact gagctaaagg tccaggctct taaaaatcgc ggcggagggg atcgaacccc
      301 cgacctcccg ggtatgaacc ggacgctcta gccagctgag ctacaccgcg aaaaagatat
      361 tcattattta aatgaaatgg agcctagcgg gatcgaaccg ctgacctcct gcgtgcaaag
      421 caggcgctct cccagctgag ctaaggcccc gtgtttaaat ttataat
//
LOCUS       DACCCM010000228          467 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCCM010000228 DACCCM010000000
VERSION     DACCCM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.238"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..467
                     /locus_tag="IVK78_002947"
                     /pseudo
     CDS             1..467
                     /locus_tag="IVK78_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          191 a           84 c           87 g          105 t
ORIGIN      
        1 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
       61 ttagctggaa agccacgtgt agaaatcatt cgagaatatg aactaacggc ttcagcattt
      121 gacaaatggg taaagcaatc taaaacgagt ggttcattca aagaaaaaga taatcttacg
      181 cctgaacaaa aagaattgtt agaactacgt aaaagaaacc agcaattaga aatggaaaat
      241 gatattttaa agcaagcagc gctgatattc ggacgaagag acaagtaatc gatgcgaata
      301 agcatcttta ccctatatca gcgatgtgca gaatattagg tctatcacgt cagtcctatt
      361 attatcaatc aaaaccaaag aaagacgaat cagaacttga agaagtagtc gctgaagaat
      421 ttatccgcag ccgaaaggcc tacggctcaa gaaaaataaa aaaagcc
//
LOCUS       DACCCM010000229          413 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCCM010000229 DACCCM010000000
VERSION     DACCCM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.239"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          163 a           51 c           55 g          144 t
ORIGIN      
        1 actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa
       61 gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata
      121 gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga
      181 agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg
      241 agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag
      301 aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag
      361 tatactagaa ttgtgataaa gtccgtataa ttgtgtaaaa gtaaaaaggc cat
//
LOCUS       DACCCM010000230          407 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCCM010000230 DACCCM010000000
VERSION     DACCCM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.240"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..384
                     /locus_tag="IVK78_002948"
     CDS             <1..384
                     /locus_tag="IVK78_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6376315.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           73 c           64 g          113 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg
       61 aataccttct tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag
      121 tcactcatag aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga
      181 accatttact caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt
      241 agaaaagagc agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat
      301 acatacaatc aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca
      361 ttagtttcaa tgtttactga gtaactaatt attttgcagg aggacaa
//
LOCUS       DACCCM010000231          406 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCCM010000231 DACCCM010000000
VERSION     DACCCM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.241"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..348)
                     /locus_tag="IVK78_002949"
     CDS             complement(<1..348)
                     /locus_tag="IVK78_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface adhesion protein"
                     /protein_id="HAP6376316.1"
                     /translation="MVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQ"
BASE COUNT           78 a          121 c           78 g          129 t
ORIGIN      
        1 ttggtcatcg atctttgtca acacgacacc gcctggtgta agtcgatttt ctttcgtcaa
       61 ttctaccacc gctgtctgac ttctttcgat ctcaaactcg attggtgtcg catccaattc
      121 ataccccgtt ggcgcttggg tttctaccag ctgatacact cctggtgcta agccgtcaat
      181 cgccagtttt ccatctgctc ctgtggttag acctgactgc agaacggttc cattggcatc
      241 ttgtaactca aaaacagctc cttgcaggac ttccccgctt tggtcatcga tctttgtcaa
      301 cacgacaccg cctggtgtaa gtcggtttgc tttcgtcaat tctaccaccg ctgtctgact
      361 tctttcgatc tcaaactcga tcggtgtcgc atctaattca tacccc
//
LOCUS       DACCCM010000232          396 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCCM010000232 DACCCM010000000
VERSION     DACCCM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.242"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..329)
                     /locus_tag="IVK78_002950"
     CDS             complement(<1..329)
                     /locus_tag="IVK78_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6376317.1"
                     /translation="MMEKFLEKISESPYRENFVLKGGFLIGSKYGIENRTTKDIDTTL
                     REMKVTKETLTTVLNDIFSTPTKEGIQFEIQGMKETREADYYPGFSLRVLAHLENMRP
                     DFKVDVTT"
BASE COUNT          108 a           84 c           57 g          147 t
ORIGIN      
        1 gtagtaacat ctaccttaaa atctggccgc atgttttcca aatgtgccag tacacgtaag
       61 ctaaaccctg gataataatc agcttccctc gtttctttca ttccttgaat ctcaaattga
      121 atgccttctt tcgtcggcgt tgaaaaaata tcgtttagaa ccgttgtcaa tgtttctttg
      181 gttactttca tttcccgtaa agtcgtatca atgtctttag ttgttcgatt ttcaatgcca
      241 tactttgagc caattaagaa gccgcctttt aacacaaaat tttcacgata aggagactct
      301 gagatttttt ccaagaattt ttccatcata aaatgtctta aaacaagctg cggatcaact
      361 tctttttctc tagaaatatt cttcactttt gctttt
//
LOCUS       DACCCM010000233          392 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCCM010000233 DACCCM010000000
VERSION     DACCCM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.243"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          150 a           50 c           75 g          117 t
ORIGIN      
        1 aatacagatg cgtatgcatt tgtgtttgcc atcttgcgtt tggtagacga gataggtata
       61 agctaaagaa acaacttgtt taaaataaac gacctagctc aacaaatgac catctaatca
      121 actaacagca tacataaaag caactatttt tgaagtagaa ttgaaatcgg gataacttaa
      181 aaagtgattg ggatactagt gagaatcacc gtacataaat attactcgtt aagggaaatt
      241 gagaaatttt gtatgaaaat ttaagaatta gttagtatgc gcaagtcaaa aatatgaata
      301 aattatttgg tggagataat ttgcttagct tgtcataaga aatgagataa tctggcagat
      361 gcaagctttt ggacaccatt tattcggaat aa
//
LOCUS       DACCCM010000234          368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCCM010000234 DACCCM010000000
VERSION     DACCCM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.244"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..>368
                     /locus_tag="IVK78_002951"
     CDS             82..>368
                     /locus_tag="IVK78_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6376318.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DACCCM010000235          368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCCM010000235 DACCCM010000000
VERSION     DACCCM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.245"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            97..>368
                     /locus_tag="IVK78_002952"
     CDS             97..>368
                     /locus_tag="IVK78_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376319.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          140 a           53 c           69 g          106 t
ORIGIN      
        1 tatgcattac agacgaagaa gcagataaaa tcattagcaa agaagagagc gcatcttaat
       61 tgatgtgctt tttattttga ttcaaggagt tgtcacatga ttaatttagg ggaatgggga
      121 gcgatagcag gatcaataac cgctatcgtt tctttgattt tattagtaat aaaaccaatt
      181 actgcatctt tctcgaagat tactgagact ctttcaaaag taagtcacaa tttagatttg
      241 ctgactaaag atttagaatc gagcaaatca gatcgattga tgattcatga agaactaaag
      301 aaacacgatg aaagattaga tacacatgca gaaaaattgg tagaacacac gcaacaaatt
      361 aaaacttt
//
LOCUS       DACCCM010000236          367 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCCM010000236 DACCCM010000000
VERSION     DACCCM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.246"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..272)
                     /locus_tag="IVK78_002953"
     CDS             complement(<1..272)
                     /locus_tag="IVK78_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376320.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          105 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcata
//
LOCUS       DACCCM010000237          362 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCCM010000237 DACCCM010000000
VERSION     DACCCM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.247"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>362
                     /locus_tag="IVK78_002954"
     CDS             <1..>362
                     /locus_tag="IVK78_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP6376321.1"
                     /translation="MQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSY
                     WQSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSW
                     FESIKDILKSIDPGGVMLA"
BASE COUNT          130 a           44 c           82 g          106 t
ORIGIN      
        1 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
       61 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      121 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      181 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      241 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      301 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      361 gc
//
LOCUS       DACCCM010000238          361 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCCM010000238 DACCCM010000000
VERSION     DACCCM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.248"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          123 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 a
//
LOCUS       DACCCM010000239          360 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCCM010000239 DACCCM010000000
VERSION     DACCCM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.249"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..233
                     /locus_tag="IVK78_002955"
     CDS             <1..233
                     /locus_tag="IVK78_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6376322.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT          135 a           63 c           51 g          111 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
//
LOCUS       DACCCM010000240          337 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCCM010000240 DACCCM010000000
VERSION     DACCCM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.250"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            122..328
                     /locus_tag="IVK78_002956"
     CDS             122..328
                     /locus_tag="IVK78_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6376323.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           82 g           92 t
ORIGIN      
        1 gataaaattg aacaactgga cgggcaaatt gatgccttgc tttcagaaag agaaatattg
       61 atagaagaaa acaagcgatt gaaaaagaaa gtagcccaac caatgaaggg aggtggatag
      121 tatgaacaat agaacctttg atattttgaa atggatcgct atagtagtta tccccgcttt
      181 agcgactttt gtcggtcttg tcggaaaagg tgttaactgg cggtacacag atataacggt
      241 gatgattatt acaggttttg gagcatttct agggagtatt ttaggtgtat caaatcgaac
      301 gtataagaaa atttcatcag ataaatagga ggataga
//
LOCUS       DACCCM010000241          335 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCCM010000241 DACCCM010000000
VERSION     DACCCM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.251"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..>335
                     /locus_tag="IVK78_002957"
     CDS             1..>335
                     /locus_tag="IVK78_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6376324.1"
                     /translation="MDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIF
                     RRYVADEDLL"
BASE COUNT          119 a           55 c           61 g          100 t
ORIGIN      
        1 atggattatt caaaagagcc agtaaatgat tactttttaa tcgatatgaa aagcttttac
       61 gcaagtgtcg aatgtataga aagaaattta gatccattaa caacagaact tgttgttatg
      121 agtcgagctg ataatactgg ttcaggattg atattagctt cttctcctga agcaaaaaag
      181 cggtatggta ttacaaatgt gagtagacca cgtgacttac cacaaccatt tcctaaaaca
      241 ctacacgttg ttccaccacg tatgaaccta tatatcaagc gaaatatgca ggtaaataat
      301 attttcagaa gatatgtggc tgatgaagat ctact
//
LOCUS       DACCCM010000242          331 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCCM010000242 DACCCM010000000
VERSION     DACCCM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.252"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>331
                     /locus_tag="IVK78_002958"
     CDS             <1..>331
                     /locus_tag="IVK78_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAP6376325.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DACCCM010000243          321 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCCM010000243 DACCCM010000000
VERSION     DACCCM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.253"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>321)
                     /locus_tag="IVK78_002959"
     CDS             complement(<1..>321)
                     /locus_tag="IVK78_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6376326.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACCCM010000244          318 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCCM010000244 DACCCM010000000
VERSION     DACCCM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.254"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>318
                     /locus_tag="IVK78_002960"
     CDS             <1..>318
                     /locus_tag="IVK78_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6376327.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSHP"
BASE COUNT           81 a           67 c           83 g           87 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatcca
//
LOCUS       DACCCM010000245          312 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCCM010000245 DACCCM010000000
VERSION     DACCCM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.255"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            22..>312
                     /locus_tag="IVK78_002961"
     CDS             22..>312
                     /locus_tag="IVK78_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6376328.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRA"
BASE COUNT          114 a           59 c           62 g           77 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg ca
//
LOCUS       DACCCM010000246          312 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCCM010000246 DACCCM010000000
VERSION     DACCCM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.256"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            88..276
                     /locus_tag="IVK78_002962"
     CDS             88..276
                     /locus_tag="IVK78_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376329.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          116 a           55 c           48 g           93 t
ORIGIN      
        1 tttacaattt aaacatttga caaactatag aacagcactt aaaatgtgta tacaagtgac
       61 agcgctttaa aaaaagaggt gcagcctatg ttaaaaaaaa ctatgttggt actaggttct
      121 atttctctag gagtattagg ttttagcccc atgctattat ccgtaattgg aaaaaataat
      181 tccaaagggg aacttaaatt aaatccaaat tactacaata gtcctaatga acctatgttt
      241 atttcagaaa aacaaaacga cggtcaggac aaataatcct gccgtcgttt tgctctaact
      301 tctaccagta ca
//
LOCUS       DACCCM010000247          302 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCCM010000247 DACCCM010000000
VERSION     DACCCM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.257"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>302
                     /locus_tag="IVK78_002963"
     CDS             <1..>302
                     /locus_tag="IVK78_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP6376330.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          117 a           48 c           68 g           69 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 ag
//
LOCUS       DACCCM010000248          290 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCCM010000248 DACCCM010000000
VERSION     DACCCM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.258"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>290)
                     /locus_tag="IVK78_002964"
     CDS             complement(<1..>290)
                     /locus_tag="IVK78_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376331.1"
                     /translation="KMISGQSKVLSVNGKYGAVILTKADLGLENAITELPYASETSDG
                     ILTAEMFQKIVNGEGGTYILPIATPEQLGGIKVGELLEVTEEGVLSATKQTD"
BASE COUNT           84 a           64 c           40 g          102 t
ORIGIN      
        1 aagtctgttt gcttagttgc agataaaaca ccttcttcag taacttctaa gagttcccca
       61 acttttatac ctcctaattg ttcaggagta gcaatcggca aaatatatgt tccaccttct
      121 ccatttacga tcttttgaaa catttcagca gtaagaatac catcgcttgt ctcgcttgca
      181 taaggtagtt cagtaattgc attctctaat cctaaatcag ctttcgttaa aatgactgca
      241 ccatatttac cattaaccga aagaactttt gattgtcctg atatcatttt
//
LOCUS       DACCCM010000249          283 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCCM010000249 DACCCM010000000
VERSION     DACCCM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.259"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>283
                     /locus_tag="IVK78_002965"
     CDS             <1..>283
                     /locus_tag="IVK78_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376332.1"
                     /translation="DDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          115 a           38 c           67 g           63 t
ORIGIN      
        1 tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat
       61 tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata
      121 ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg
      181 ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag
      241 acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DACCCM010000250          276 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCCM010000250 DACCCM010000000
VERSION     DACCCM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.260"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>276
                     /locus_tag="IVK78_002966"
     CDS             <1..>276
                     /locus_tag="IVK78_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP6376333.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKG"
BASE COUNT           97 a           56 c           52 g           71 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcat
//
LOCUS       DACCCM010000251          268 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCCM010000251 DACCCM010000000
VERSION     DACCCM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.denovo.261"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            87..>268
                     /locus_tag="IVK78_002967"
     CDS             87..>268
                     /locus_tag="IVK78_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6376334.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           86 t
ORIGIN      
        1 tttacctatg aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac
       61 tttttaacga ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa
      121 ctttggctgc cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata
      181 ccgttcaatc aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct
      241 tgatcaatgc cattgcagaa tcaaattc
//
LOCUS       DACCCM010000252        35875 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.018, whole genome shotgun sequence.
ACCESSION   DACCCM010000252 DACCCM010000000
VERSION     DACCCM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.018"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            40..504
                     /locus_tag="IVK78_002968"
                     /pseudo
     CDS             40..504
                     /locus_tag="IVK78_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
     gene            704..1018
                     /locus_tag="IVK78_002969"
     CDS             704..1018
                     /locus_tag="IVK78_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6376335.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1039..1416
                     /locus_tag="IVK78_002970"
     CDS             1039..1416
                     /locus_tag="IVK78_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6376336.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1426..2199
                     /locus_tag="IVK78_002971"
     CDS             1426..2199
                     /locus_tag="IVK78_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376337.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2221..3624
                     /locus_tag="IVK78_002972"
     CDS             2221..3624
                     /locus_tag="IVK78_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6376338.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3806..4990
                     /locus_tag="IVK78_002973"
     CDS             3806..4990
                     /locus_tag="IVK78_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6376339.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4987..5277
                     /locus_tag="IVK78_002974"
     CDS             4987..5277
                     /locus_tag="IVK78_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376340.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5274..5495
                     /locus_tag="IVK78_002975"
     CDS             5274..5495
                     /locus_tag="IVK78_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376341.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5537..6316
                     /locus_tag="IVK78_002976"
     CDS             5537..6316
                     /locus_tag="IVK78_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP6376342.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6383..6877
                     /locus_tag="IVK78_002977"
     CDS             6383..6877
                     /locus_tag="IVK78_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6376343.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6933..7571
                     /locus_tag="IVK78_002978"
     CDS             6933..7571
                     /locus_tag="IVK78_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376344.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7643..8038
                     /locus_tag="IVK78_002979"
     CDS             7643..8038
                     /locus_tag="IVK78_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6376345.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8022..10475
                     /locus_tag="IVK78_002980"
     CDS             8022..10475
                     /locus_tag="IVK78_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6376346.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10472..12499
                     /locus_tag="IVK78_002981"
     CDS             10472..12499
                     /locus_tag="IVK78_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376347.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12496..13518
                     /locus_tag="IVK78_002982"
     CDS             12496..13518
                     /locus_tag="IVK78_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6376348.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13535..14464
                     /locus_tag="IVK78_002983"
     CDS             13535..14464
                     /locus_tag="IVK78_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6376349.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14709..14825
                     /locus_tag="IVK78_002984"
     CDS             14709..14825
                     /locus_tag="IVK78_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6376350.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14841..16760
                     /gene="tet(M)"
                     /locus_tag="IVK78_002985"
     CDS             14841..16760
                     /gene="tet(M)"
                     /locus_tag="IVK78_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6376351.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            16858..17046
                     /locus_tag="IVK78_002986"
     CDS             16858..17046
                     /locus_tag="IVK78_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6376352.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(17104..17457)
                     /locus_tag="IVK78_002987"
     CDS             complement(17104..17457)
                     /locus_tag="IVK78_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6376353.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            17988..18410
                     /locus_tag="IVK78_002988"
     CDS             17988..18410
                     /locus_tag="IVK78_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6376354.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18407..18637
                     /locus_tag="IVK78_002989"
     CDS             18407..18637
                     /locus_tag="IVK78_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6376355.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            19135..19335
                     /locus_tag="IVK78_002990"
     CDS             19135..19335
                     /locus_tag="IVK78_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6376356.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            19362..20555
                     /locus_tag="IVK78_002991"
     CDS             19362..20555
                     /locus_tag="IVK78_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6376357.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(20623..22188)
                     /gene="guaA"
                     /locus_tag="IVK78_002992"
     CDS             complement(20623..22188)
                     /gene="guaA"
                     /locus_tag="IVK78_002992"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6376358.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(22247..23170)
                     /locus_tag="IVK78_002993"
     CDS             complement(22247..23170)
                     /locus_tag="IVK78_002993"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6376359.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            23366..23971
                     /locus_tag="IVK78_002994"
     CDS             23366..23971
                     /locus_tag="IVK78_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6376360.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            24279..25295
                     /locus_tag="IVK78_002995"
     CDS             24279..25295
                     /locus_tag="IVK78_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6376361.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            25436..26737
                     /locus_tag="IVK78_002996"
     CDS             25436..26737
                     /locus_tag="IVK78_002996"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6376362.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            26783..27445
                     /gene="deoC"
                     /locus_tag="IVK78_002997"
     CDS             26783..27445
                     /gene="deoC"
                     /locus_tag="IVK78_002997"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6376363.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            27466..27864
                     /locus_tag="IVK78_002998"
     CDS             27466..27864
                     /locus_tag="IVK78_002998"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6376364.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            27930..29012
                     /locus_tag="IVK78_002999"
     CDS             27930..29012
                     /locus_tag="IVK78_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6376365.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            29224..30786
                     /locus_tag="IVK78_003000"
     CDS             29224..30786
                     /locus_tag="IVK78_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6376366.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            30779..31912
                     /locus_tag="IVK78_003001"
     CDS             30779..31912
                     /locus_tag="IVK78_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6376367.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            31912..32868
                     /locus_tag="IVK78_003002"
     CDS             31912..32868
                     /locus_tag="IVK78_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6376368.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            32967..34133
                     /gene="deoB"
                     /locus_tag="IVK78_003003"
     CDS             32967..34133
                     /gene="deoB"
                     /locus_tag="IVK78_003003"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6376369.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            34157..34975
                     /locus_tag="IVK78_003004"
     CDS             34157..34975
                     /locus_tag="IVK78_003004"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6376370.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            34992..35699
                     /gene="deoD"
                     /locus_tag="IVK78_003005"
     CDS             34992..35699
                     /gene="deoD"
                     /locus_tag="IVK78_003005"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6376371.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11803 a         6235 c         7439 g        10398 t
ORIGIN      
        1 ttgatgcttc acttttacac aattatatgg acgtaatccg acaacggtgt tgatttatac
       61 aaatttgtta ccaaacatat atttgttaat cttaatattg acgagaaaaa ttacccgtct
      121 atttttaagc actatctatt ttttattaac gaagtagaag atcggataaa aaataattca
      181 ttatctagta gtgtatctag ttttaagaag atgtttaaat ctcgagatgg tatcacactt
      241 actacagcac ataaagttaa aggaaatgag tatgatactg ttatcgctat taatcttcta
      301 aacgggaaaa ttcctcattg gaatgaaatt tttaatgttt ctgataaagg aattagttca
      361 gcgagaaaac tactatacgt aatttgttca agagcaaaaa ataatttatt tcttttctca
      421 gaggtgggtt attttacgag taaaggagac ttgctttatc ccactcaatt attagataaa
      481 atagaatttg attataatat ttaaaatacc taggtagtcg ttgttttaaa tatagtagga
      541 atcggacgct cagtacggag cgtctatttt tttacccaat tttgaaagga agtgaactta
      601 tgaaaataaa aaatcaagaa tcaaaaggtc gctctccccc ctttaaaacc atcacacaaa
      661 taagattcat tcaaacataa aaacagaaag gataggtaaa aatatggaac ttaaatatgt
      721 cattcccaat atggaaaaaa catttggaca attagaatat gctggtgaag ggaacattga
      781 acaacgtcga ataaatggca gaccaaccat tctctctcgt agttacaatt tatactcaac
      841 gattcaacga gcagatgata ttgttgtcat tcttccctcc gaagctggcg agaaacattt
      901 tgaatccgac gaaaaagtca aactcatcaa cccacatatt accgcagaag gttataaaat
      961 tggcaatcga ggatttacca attacattat gcacgcagac gatatggtga aagcataaga
     1021 aaggaaaggt gaaaaaatat gagattagca gaaggtattg tgattgaaaa agaccagaca
     1081 tttggcacat tgaaattttc cgccttgcgt cgtgaggttc gtatccagaa tgaagatggt
     1141 acggtttccg aagaaattaa agaacgtacc tatgacttaa aatcaaaagg acaaggacgc
     1201 atgatacaag tcagtatccc cgcttctgtt cctttgaaag aatttgacta caacgcagaa
     1261 gtggaattaa tcaatcctgt ggcagatacc gtagcgacgg ctacttttcg aggggcagac
     1321 gttgattggt acgtgaaagc agacgatctt gtattaaaag gacagacaaa acctcatcag
     1381 aaatataaaa aagaagcgtc cgaaagtgaa aaataggtgt tgcttatgac aaacacacca
     1441 ttgattttga aagaaattcc gaaggatgaa gccatttctt ttattcggca ataccattat
     1501 tcaaagatac tccctagatt gtgtaaatac tttctaggga ttttttcaga agaaaaatta
     1561 ttaggcgttg tggaattagg ctggggtacg caacccctac aaaccatacg caaactcttt
     1621 cccgacagtt cccttcaaac cacagattat ttagaaattg gaaagatgtg ttttcttcca
     1681 gaaatgaatc aaaccaatta ttttggcagt caagctcttt cagctctaat taagtggttg
     1741 aaagaacata cggactgtca ttttttatat acactggcag atggaattga gggaaaatgt
     1801 ggctatgtct atcaagcgtc caacttcttt tactgcgggt atttcaagac aagtgtctat
     1861 cgtgataagc agtcatggga aaagatacac cctcgaagtg ctcgcctttt attagaggaa
     1921 aatgctcgct ttgaacaggt agagaaaaag cattggcttt cccaggcatt ttgcgaatac
     1981 aaagggattg agaaaatcaa tggacgcatg tttcgctatc tctatccgtt gacaaaagaa
     2041 gcgaaaaaac tgttaggaca cactctctat cgacgtcatt attatccgaa agaaaaaaat
     2101 ttacgctttg aaaagcgaat cgcttatcag aaatatgaag ccatttctca accaactttt
     2161 gataaacagg ctcgtatcta caatacacaa ttattttgat aggtggaaag gaggattttt
     2221 atgccattgc ttcatgacag aggagagcga atcaaagcca acgataagca cttggtctat
     2281 cattttgtac taatttcatt gctggtagtt tttctagtcg tcctactccc ttttcattgg
     2341 agagcgttac aatcggtgaa ctggcaagca gttcagttag atacattttt ctatcaactt
     2401 catacgccat acttttggag cagtattctc actgctcttt ttgtatgcgg acttatcagc
     2461 tggctttatt atcactatcg tatagattct ataaaacagt tagagcaccg tcaaaagtta
     2521 gctcgtatga ttttagaaaa tcattggtat gaagccgaag aaactcaatc agagggattc
     2581 tttaaagatt tacccagcaa gaaaaccaaa caacgaatcc gccattttcc taaaatatat
     2641 tatcgaatga aagatggact aatccatatt catgtagaaa tcaccttagg gaaatatcaa
     2701 gatcagcttt tgcacctaga aaacaaacta gaaacaggat tgtattgtga attagtggaa
     2761 aaaacgttga aagattctta tgtagagtat gtcctacttt atgatactat tggcaatcga
     2821 attggcattg aagacgtggt agctcaagat ggacgtgtca aattgatgga atcggtctat
     2881 tgggagttcg attctctccc tcacatgtta attgctgggg gcactggtag tggaaaatcc
     2941 tattttattc tcacattgat tgaatctctc ctgcatacaa atgcaaagct ttatattctt
     3001 gaccctaaaa atgctgattt ggcagactta ggggcggtca tgaataatgt ctattaccgt
     3061 aaagaagata tgttggcatg tatcaatcaa ttttacgaag atatgattga tcgcagtgaa
     3121 accatgaaac aacaccctaa ctataaaaca ggagaaaatt atgcttatct tggtttgtct
     3181 gccaattttc ttatttttga tgaatatgtg gcaatgatgg atatgctagg tcgtgaaagt
     3241 accaccgtta ttcataaact caaacaaatt gtaatgttag gacgacaagc aggcttcttt
     3301 ttaattcttg cctgtcaacg tccagacgca aaatatttta gtgatgggat tcgggacaac
     3361 tttaacttcc gtgtggcttt gggtcgtatg tctgaaatgg gctttggtat gatgttcgga
     3421 agcgatacac aaaaacaatt tttcttaaaa cccatcaagg gaagaggtta cgtggataca
     3481 ggaaaaagtg tgattagtga gttttataca ccccttgtac caaaaaggta cgatttctta
     3541 ggagaaatag gaaaagtcat tcaaaaaaag caatctgagc ctgtaccgca cgaagtgaaa
     3601 ggtacaggtt cagacttgct ttgattggtg tggcgttagc cacgccaatc ctttagcccc
     3661 ctctatctaa caggggggca caaatacaac tgaaaacagg caattctcat caaccaatgt
     3721 tgatggtaca aaggattgcg gaaaatatga aaggtgtaaa ctcaagggtc atagtttaca
     3781 tcttttttag attggaggtt tgcgaatgga aaacgaaaaa atttggatta aggaattgaa
     3841 agaaaagcga ctggcttatg gcgtttctca aaataaactg gcggttgctt ctcatattac
     3901 cagaccttac ctatcggata ttgaaacagg caaggcagtc cctactcaac aggtgaaaga
     3961 agatttactg aacgctttgg aacgcttcaa tcccgataac ccattggaga tgttattcga
     4021 ctatgtacgg attcgattcc ctaccaatga tgtggctcaa attatcggtg aggttttacg
     4081 tttgaatatg gattatatgc tacacgaaga ctttggctat tattcttatc ccgaacacta
     4141 tcgtttcggt gatattgttg tcctagtctc tcatgatgta agtaaaggtg ttttattaga
     4201 actgaaagga aaaggctgtc ggcaatttga aaactttcta ttggctcaac atcggagctg
     4261 gtatgatttc tttcaagatt gcatggaaca taaaggtatt ttcaaacgct tggatttagc
     4321 tatcaacgat aaaacaggga ttttgaatat tccagatttg gcaaagaaat gtaaacagga
     4381 agaatgcatt tccgtctttc gaagctttaa aaattatcgc agtggtgaat tggttcaccg
     4441 tgatgaaaaa ccatacatgg gaaacacact ttatattggc tcattaaaaa gtgaagtcta
     4501 tttctgtata tatgagaaag attatgagca atacgtgaaa aatgatgttc ccttagaaga
     4561 cgcagaagta aagaatcggt ttgaaattcg gttaaaaaat gaccgagcca cccaagccat
     4621 gaaagattta ttggcacatc aacaggcgga aaaaacagcc tttgaaatta tcaatcgcta
     4681 tatccgcttt gcagataaag acgacactaa acgtcggagt gattggaaaa ctaatgaacg
     4741 ttgggaatgg tttatcggta aaaatcgtgg agcattacgc ctaaccacac agccagagcc
     4801 ttattctttt gaacgaacat tgaactggtt acatcatcaa gtcgccccta cattaaaaat
     4861 cgcaagcatt ttagatgttc tcaacggtac aaccattatc tccaccatga ttcaggaagc
     4921 caaactgaca gaaaaacatg agaaattaat tgaacaacaa catctagcca tggaggattt
     4981 gattacatga gccttttaac aaaagaatta aagaagttag ggtttcagtg tggcattgag
     5041 tttcaagcgt atatacaaaa tacagggaaa tatacgtctc tcattattga gggaaaacgg
     5101 caagctggtg ataccatcta tacctatgat ttttacaagg tgagatttta taataattat
     5161 accaatcgag taaccgtcta tggagagcat ttaacccctt ttcaattatt aaggcgtgta
     5221 aaaagctata tctattatcg agaaaaatat cttaaggaaa ggaggacaat cacatgaatt
     5281 ttgggcagaa tttgtacaac tggttcttgg ataatgcaca gtcattggta ttgttagcga
     5341 ttgttgtcat tggactgttt ttaggtttta aacgagaatt ttcaaaactc attggcttct
     5401 tgattattgc tctaatcgcc gtcggacttg tgttcaatgc agctggcgtg aaagatgtgt
     5461 tattaaactt atttaaccga atcattggtg cttaacagat atttttcatg ataaaatagg
     5521 aagtatatat tttgttatga aaggatttat gaatatgttt acaaatgatc aatttaaatc
     5581 tcttttaatt aaaacattag aaagaaagag ctcttcaaat tattatgaag gaggtaacga
     5641 gattgtttct aaaatgaaaa tatcagaggt aactttagat gagactgacg attttactta
     5701 ttataaagga tataaaaagg gctggaatac gctttgcaca tatcttaaaa taagagttcc
     5761 ttttgatgat ttagactttt ttgaaagtca taaagatttg attacccaga cagcaagctc
     5821 catatatgat aaacaaggtg ataatgtatt agtggatact attttagtac cacttccaga
     5881 gaattacgaa gtaattaact ttagtcagtt gaaaattagt gatgtggttt cacaagctat
     5941 tgaagacgct gagtctttta tgagtaatgg tgaatatcaa cgtgcctttg acagagttca
     6001 cactgctttt catggatatt taattgaaat actaaaaaaa tatgaaatta cagttcctag
     6061 agacgaaaat ctatctaaat tatatagtag gatacaacaa ttaattgaaa aagaaattca
     6121 acctactgaa cttgctgata tagtgaagac aacgataagg tcatcaaatg gaatgattag
     6181 ctctcttaat gaagcaagaa ataggcattc acttgcacac cctaatacaa atattattgg
     6241 aaagagagaa gcaaaactaa taataggtat ttcaagtaca gttacagatt atattagcgg
     6301 ataccttgat aagtagcgtc attaactgat tgctactttt tttatgacta tttttataaa
     6361 aaggatgtga taagaattgg aaatgaaagt ctatgtggca aatttaggac ggtataatga
     6421 aggtgaatta gtcggagcat ggtttactcc accaattgat gaggaagaaa tggcagaaag
     6481 aatcggttta aatgaggact acgaagaata tgcaatacat gattttgagc ttccttttga
     6541 tgtggacgaa tacacgccga ttagtgaaat caaccgtcta tgtgaagcca ttcaagaaat
     6601 cgaaggtaca cctatttata atgaactcaa agaaattcaa ggcatgtggt ttagtagctt
     6661 ggaagaatta ctggaaaaca aagaggatat tcattgttat agtgattgtg attcgatgga
     6721 agatgtcgcc cgttattacg tggaagaaac aggacagctt ggtgaagtcc cttccaattt
     6781 acaaaattat attgactacc aatctttggg acgtgatatg gaaatagagg gtaattacct
     6841 agttacctct catggggtat ttgaatattg tcagtaagtt taacaatccg tgttataatt
     6901 aaattaataa atagcacgga gaggtataaa ttatggtaaa tgtatttgaa aatcttaaac
     6961 tattaaaact agacatgaaa gataaaggct gggtgataga ttccttttac tttcgttaca
     7021 aacagcaaaa ttatattgta cttgttaagc tatttgaaaa agaggaaaaa gttccagaat
     7081 atgctctgtt aaaactagaa tttttaaagg aaaacgattt ttcagatatg ctagctgtct
     7141 atgctaattc ggtaaaatta tatacagaca cgaaaacaat aagagaatat tttggtatcg
     7201 aatacagtag taacttggga gatgttttat ttcaatttag ccaaactctt gctcgattta
     7261 ttccgacaga agttagtgag aagaaaaatg aagaccaaaa agaagccatg tgtttctcat
     7321 taagtcaaag tgattcagaa gatcccagaa aaaaatattg tttttcagtt agaagaaatc
     7381 ctttaaaggg aaatggtgag ctgggaaaac gaagtccttt taatgacaat aaaacgagat
     7441 tgtatagacc cagcttatat gaacgattag ggtcagatac caatttaagt ttcagatatt
     7501 caatgaatcc agatgatgaa gaaactgatg aaggtattat tgcaaaatgg acaaaaaata
     7561 aaattgaata gttaaagcat tgaaagagca atcacctatt ttgattgttc tttttctatt
     7621 tttacggaaa ggacgtgaag acatgaaaaa aataagaagt tacaccagta tttggtcagt
     7681 agaaaaagtg atttatgcga ttaatgattt acaactccct ttcccaatta cctttacgca
     7741 aatgacatgg tttatcgttt ctttgtttgt cgtcatactc tttggcagtt taccaccact
     7801 ttctctgatt gacggagcac tactcaaata cttagggatt cccattgttc tcactgggtt
     7861 catgtgccaa aagacctttg acggaaagaa accctatgga tttctaaagt ctatcattac
     7921 ctatgctcta cgccctaaag taacctacgc tggcaaacaa atacgattaa aaaaagaaac
     7981 aatgaatgaa tcgattacga ttgtaaggag tgaacaacta gatggcatat ccgattaaat
     8041 atatggaaga taatctcgta tttaatcatg atggcgaagt attcgcttat tatgaattaa
     8101 ttccctataa ttacagtttt ttaagttccg atgaaaaaat tcaagttcat gataatttct
     8161 gtcaattgat tgcccaaaat cgtgatggca agattcatgc tctgcaacta gcaaccgaat
     8221 caagtattcg agccacacag gaacaatcga aaaaagaaat cactggacga ttaaaagaga
     8281 tagcttacga aaaaatagat gaacagaccg aagcgttaat ttctatgatt ggtgacaatc
     8341 aagtagacta tcgctttttt attggtttta aactctacct caatgaacag gaagtatccg
     8401 taaaaaatgt tgggaaagaa attcaaaaag catttgcgga attctttcat ggtgtgaatc
     8461 acaaattaat gggcgatttt gtttctatgc ctatggcgga aatcgaacgc ttttctaaga
     8521 tggaacagtt gttagaaagt aaattatcca gacgctttca gattcgtaaa ttggagaaag
     8581 atgattttgg ttatattatt gaacatcttt acgggcaaac aggtgtccct tttgatgact
     8641 acgactacca tttaccgaaa cgatacgaag ataaaaaagt gttggtaaag aaatacgata
     8701 ttctcaaacc cactcgctgt ttaatcgaag aaaatcaacg ctatttacgg attgaacagg
     8761 aagataccac tacttatgtg gcgtatttta caattaatag tattgttggt gaattggatt
     8821 tccctaatag tgaaattttt tattaccagc aacagcagtt tactttcccc gttgatacca
     8881 gtatgaatgt tgaaattgtc gccaataaaa aagccttatc taccgttcgc aataaaaaga
     8941 aagaactgaa agatttagat agccacgcat gggaaagcga caacgaaacc agtactaatg
     9001 tggtggacgc tttagaatct gtcgatgaat tagaaagtaa tttagaccaa accaaagaat
     9061 ctatgtacaa actaagctat gtggtacggg tatcagcaaa gaatttggac gagctgaaac
     9121 aacgttgtga tgaagtaaaa gatttttatg atgatctgtc tatcaaatta gtacgacctt
     9181 ttggcgatat gctaggcttg cataatgaat ttattccctc aagcaagcga tatatcaatg
     9241 actatattca atatgtcacg tcggattttt tggctggtct tggtttcggt gctacacaaa
     9301 tgattggtga aaattctggt atttacgtcg gttacaatct cgatacaggc agaaatattt
     9361 atctccaacc tagccttgca agtcaaggaa ttaaaggctc tgtaacaaat gcattagcgt
     9421 ccgcttttat tggttcatta ggtggcggaa aatcattctc taacaatatg ctggtctatt
     9481 attcagtact ctttggcgga caggcaacca ttgttgaccc aaaagctgaa cgtggtgaat
     9541 ggaaagaaac cttacctgat attgcccatg aaatcaatat tgttaatctc acgagtgaga
     9601 acgacaataa agggttactt gacccttatg tgattttaaa actcccgaaa gattcagaaa
     9661 gccttgccat tgatattctc acgttcttaa caggtatttc cagccgagat agcgagaagt
     9721 ttcctgtcct gcgtaaagct attcggagtg taactcaaag caaacaacga gggttgttat
     9781 tggtgattga tgaattgcga aaagaagata cgcccatcag caatagtatt gctgaccata
     9841 tcgaaagctt tgtcgattat gattttgccc atctgttatt cagtgatggc agtgtcaccc
     9901 aatccattag cttggaaaaa caactcaata ttattcaagt ggcagatttg gtcttacccg
     9961 attccaatag ctcttttgaa gaatacacta ccatggaatt attaagtgtg gcaatgctga
    10021 ttgtcattag tacctttgct ttagacttta tccactctga ccgttccatt tttaaaattg
    10081 ttgaccttga tgaggcttgg agttttttac aagtggcaca aggaaaagct ctatctatgc
    10141 gattggttcg agcaggacgt gccatgaatg caggggtcta tttcgttact caaaacgcgg
    10201 acgatttact agatgaaaag atgaagaata atattggctt gaaatttgcc tttcgttcca
    10261 ctgacctagt ggaaatcaaa aagaccttag aattttttgg tgtggacaag gaagatgaaa
    10321 acaatcagaa acgcttacgt gaattagaaa atggacaatg tttagtttca gacatctatg
    10381 gtcgtgtagg ggttatgcag tttcacccga tttttgaaga gttgcttcat gcctttgata
    10441 ccagaccgcc tgtgcgaaat gaggtggaag aatgacacaa aaacaaaaga aatttcttca
    10501 ctatagttgg ataacgctcc tttgtgtggg cgttatttta cttttattag gcacgctggg
    10561 caatgcagta caagcgacag gcttagttga tgaaaccatt gatacaagca atgaatactc
    10621 caagtatggt ttgaatcaat atcagcttga ttattatgtg gacaatagct ggggctggct
    10681 tccatggaat tggagtgatg gcataggaca atcggtgatg tatggattgt atgccattac
    10741 taattttatt tggacaattt ctctttatct atcgaacgcc acaggttatt tggtgaaaga
    10801 agcctatgcc ttagatttca ttagtcaaac atcccaagca attggcgaaa acatgcagac
    10861 gattgcaggc attacaccga atggcttttc cagttcaggg ttttatgtcg gtttcctcct
    10921 tctttttatt ctcattgttg gaatctatgt tgcctataca ggattggtta agcgagaaac
    10981 gacgaaagtg atacgagctg ttttaaactt tgtcgtggtg tttattctat cggcttcctt
    11041 tattgcgtat gcacccgatt atattgcgaa aatcaatgat ttttccaaag atgtcagtac
    11101 cgccagtctg aatattggga caaaaattgt tcttcctaac acggatagtc aagggaaaga
    11161 cagtgtggac atgatacgtg acagcttatt ctctgttcaa gtaaagcaac catggctatt
    11221 gcttcaatat ggctccactg atgtagaagc gttaggggaa gaacgtgtgg aaagtttatt
    11281 gtctaccagt ccagatacaa acaatggtga agatagagaa aatgtagtga ttgaagaaat
    11341 tgaagatcat gacaatacct atttaaccct accgaagacg atttccagat taggaacagt
    11401 cttctttttg tttgtattca atattggtat ctcgattttt gttttccttc taacaggaat
    11461 catgattttt tcgcaagtgc tatttattat tttcgctatg tttttgccaa tcagtttcct
    11521 attaagtatg ttgccgagtt ttgatggcat gggcaggcat gccattacca agttatttaa
    11581 tgtgattatg acaagggctg gtattacctt gattattacc gttgccttta gtatttccac
    11641 tatgctttat tcgctgtcag caagttctcc ctttttcatg attatgtttt tacaaattgt
    11701 gacctttgca gggatttatt ttaaactggg cgatttaatg agcctattca ctttacaaag
    11761 caacgactct caaaacatgg gcagacaagt gtttcgcaga cctcgtcaaa tgatgaatcg
    11821 acaaaccaga agatttcaac gcagtatgag taagattttt cgtggaaaag cagggtcaac
    11881 gaagcaaaag acaatgacaa caaagcagaa tcaaaccaat aaagctaacc atactcgtca
    11941 aaatgaacgg actacaccaa agaaagaaac agcactttcc aatgtgaaaa agcaacaagg
    12001 cggtatcggg caacgattag gtgaaaagac agcccatgta ttggacacga aaaatcgtct
    12061 agtcaataaa agaaagcagg caaaacaaca gatacaggcg acaccaaccc atgtcaaata
    12121 tgcccttcat caaggaaaag agaatgtcac tcataatgta acagacttta aacaatctat
    12181 ggcagatacc aaagctcaac gcaaacaaca gcgtatgaac cagcaggagc aaaaacgaaa
    12241 gaacattgct caaaagcgtt tggagctgga taaagcaaaa gaaaaaagac gaaaagaaaa
    12301 cccatcatct cctgttcaga ataaccagca gcatgttcgc aagcgtcagc aaaccgtcac
    12361 gcctgttttg tcacaaaaca ctaaacgtga aaatcatcag cgaccattga aaaaagagca
    12421 aaaacaaaag acaccaccga agcaattcaa tcagaggaag ctaaataaaa gaacggataa
    12481 gcgaggacaa caaacatgaa gctaaaaata acagtgattt gtagtgtggt cttctctctc
    12541 ttcttttttc tcttattatt cctagtcatt ttctttgcag atgatacgga cagtggcgaa
    12601 aacaacaagg attcttctat tccacaaggc ggtgtgaccg tttcaccaga agtgttggca
    12661 caccgtccgt tgattgaaaa atatggtaag gaatatggga ttgaagacta tgtttcttat
    12721 atcttagcca ttatgcaagt ggaatcgggc ggtacagcag aagatgttat gcaaagttcc
    12781 gaatctttgg gtttaccgcc caacagtttg agtaccgaag agtccatcaa acaaggggtt
    12841 aaatatttca gtgaattatt aaccagtgca gaacaacaag gagtggatat agattctgtt
    12901 atccaatctt ataattatgg cggtggcttt ttaaattatg tgaggagtca tgggaaaaaa
    12961 tatacctatg aattagccga acagttttct aaagagaagt cgggtggtca aaaagcagac
    13021 tatcccaatc ctatagccat acctgtgaat ggtggttggc gatataacta tggaaatcag
    13081 ttttatgttc agttggtttc acaataccta accgacactt ccccaacaga atttgatggt
    13141 gaaaccgtgc aagtcattat ggacgaagcc ttgaaatacg aagggtttcc ctatgtcttt
    13201 ggaggggcgt cgcctaccac ctcttttgat tgtagtggct taatacaatg ggtgtatgac
    13261 aaagctggca tttctctccc tcgtgtcgcc caagatcagt atgacgctac gcaagaaatc
    13321 tctatggaag aagcccaagc aggcgacctt atcttctttc attcaaccta taatgcaggc
    13381 acatatgtga ctcatgtggc gatatattta gagggaaatc gcttttatca tgcaggcgac
    13441 ccaatcggtt atggcgactt aagcagtcgt tattggcaag accatctgat tggggctaga
    13501 cgtgttattc ataactaaga aaggaatgaa tcaaatgatt ataaaaatcg agcgaaaaga
    13561 aaagaaggag aaaccaaaga aatctaaaaa accgagcaag caaagaaaag ttccgatgat
    13621 taaagttggc acgcataaga aattaacctt tgttttatgg gttttattga ttggtagtgt
    13681 cggatttgga atctataaaa attttactgc cattgacact catactgtcc gtgaaacaga
    13741 aatcattaaa cagcagattg tcgataccaa ccaagtagaa agttttgtga aatcctttgc
    13801 gaaagactac ttttcgtggc aacaatccca agaagccatt gataaacgaa atgaaaaatt
    13861 gacccattat ttgacggaag aattgcaggt attaaatgaa gaaatgattc gtaaggatat
    13921 tcctactagt tctagtgtga acgatataca ggtgtggcag gtttctcaag tgaatgagaa
    13981 cacctttgag gtactgtttt ctgttgaaca agttattacc gaagacaaag acaaggaaac
    14041 tatatcgtct agctttcatg tggttgtcca tatagatgaa tcagataata tggttattat
    14101 caaaaatccg acaatgagta agaaacctca aaaatcggac taccaaccca aacagctaga
    14161 aagtgaccat acagtagata cagaaacgat ggacgaaatc actagctttt tagaaacgtt
    14221 cttccagctc tatcctaccg ccacagaaaa agaattgact tactatgtaa gtaatcatgt
    14281 gttacctatg attaacaagg aatatgtttt cgaggaattg gtgaatccta tctttaccag
    14341 aaaagataac caagtaatcg tgaatgtcgc tgtgaaatat ttagatcaag aaacaaaggc
    14401 aacccaaatc tcgcaatttg agttgatatt agaaaaacaa gataattgga agattgtaaa
    14461 ataacaaata ttggtacatg attacagata ctttgtaatc atgtactctt tttgataaaa
    14521 aattggagat tcctttacaa atatgctctt acgtgctatt atttaagtga ctatttaaaa
    14581 ggagttaata aatatgcggc aaggtattct taaataaact gtcaatttga tagcgggaac
    14641 aaataattag atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc
    14701 tttatcatgt gattctaaag tatccagaga atatctgtat gctttgtata cctatggtta
    14761 tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc cttttgggtt
    14821 tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg
    14881 caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag
    14941 gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt cagagaggaa
    15001 ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg aacatcatag
    15061 acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca gttttagatg
    15121 gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattatttc
    15181 atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt gaccaaaatg
    15241 gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc gaaattgtaa
    15301 tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc gaatctgaac
    15361 aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg tccggtaaat
    15421 cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat tgttctctgt
    15481 tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt atagaagtta
    15541 ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt
    15601 tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt tatagtggag
    15661 tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa
    15721 tgtatacttc aataaatggt gaattatgta agattgatag agcttattct ggagaaattg
    15781 ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc
    15841 cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta
    15901 aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc
    15961 ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag
    16021 tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa
    16081 aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca
    16141 tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt
    16201 tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc
    16261 aaaatgcagt tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg
    16321 tgacggattg taaaatctgt tttaagtacg gtttatacta tagccctgtt agtactccag
    16381 cagattttcg gatgcttact cctattgtac tggagcaagc ctttagaaaa gctggaacag
    16441 aattgttaga gccatatctt agttttaaag tttatgcacc acaggaatat ctttcacggg
    16501 catataacga tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg aaaaataatg
    16561 aggtcattat tattggagaa attcctgctc gatgtattca agattatcgc aatgatttaa
    16621 ctttttttac aaatgggctt agtgtttgtt tagcagagct aaaaggatat caggttacca
    16681 ctggcgaacc tgtttgccag acccgtcgtc taaatagtcg gatagataaa gtaagatata
    16741 tgttcaataa aataacttag tgcgttttat gttgttatat aaatatggtt tcttattaaa
    16801 taagatgaaa tattctttaa tatagatttg aattaaagtg gaaaggagga gattgttatt
    16861 ataaactaca agtggatatt gtgtcctatt tgtggaaata aaacaagact acgaatacga
    16921 gtggatacta tacttaaaaa tttcccttta tactgcccca aatgtaagaa cgaaacttta
    16981 attaatgttc aaaaaatgaa tataataaca atcaaagagc cagacgccaa gacgcagagc
    17041 cgataatttg agaaatgaaa ctctcatctt atcggctctt tttgtttatc tgaattttac
    17101 tgactagcct tcaatatttc ttgcttcatt gatacctttt gctagtgctt ccattaagga
    17161 tagttctttg tctgtaaagc tatccatgta tttttctacc tgtaaccttc gagtgctttt
    17221 aatcaaatta tctgtaggta agaaaaattc atcaatagat acatggagta atgatacaag
    17281 atcatacagc acttgtgtac ttgggtgttg tcctttgttt tctatatttg ttaaataacg
    17341 tgggtcaatt tcaatcattg ttcccacttg ttcacgagtg agaccccgtt tcattcgagc
    17401 ctctttaatc gctaaaccaa cggctctaaa atcgtattta tcttcttttt tacgcatact
    17461 aaatcacctc tatacatttt actgttccta tataaccata aacaggtata gaaaaacatg
    17521 ttatatagtt tatgagttcc tatagaacac tgtggaattt attaatgcga tgaatccaat
    17581 ttcaaataga aaggtcgctg attttattta cctatacaac tttataaaat ttagtcatac
    17641 tcctagaaag tagataaaat tggcgactta atggagggaa aagtcgccat gagaaagata
    17701 atctgtcatc tagttctctt aattaagttc ttatcaaaaa aacttatcca cctgtactgg
    17761 atagatatga ccatcaatgt ggttttgcag attacataga tagaaaaatc aaagcatatg
    17821 aacagagaaa acaatctgtt tatgtgcttt tttggtcact taataaatat cttttaggat
    17881 ttctcttcat ttatgcaaca aatccccctt tcgtgtcggg gtttagtatg aagggagata
    17941 aagaagcaca tgccactccc tttcacgaca gaaagggggt gaaaagcatg aaaccatctt
    18001 cttttcaaga aaccatagaa aatcagtttg actatatctg caaacaagtt attgaagatg
    18061 aacgaaaaga ttacttcaaa catctagaca gactatcagc aaaagaagtc tcgttttctg
    18121 atatagataa ctaccttgtc aactcttttt ctactgttga tacgtacgtc actgatttcc
    18181 aactatttaa attatttgga ttaagaatca atgttgaaag tgatttatta agtgaagccc
    18241 tgcgtcatct ttcagagaaa aaacggaaca ttattttact tcattacttt atggatatga
    18301 gtgatgtgga gattgcggag ttactaaatt tgaatcgttc gactgtttat cgtcatcgaa
    18361 taagtggact agccatgatt aaagaattca tgaaggagtg taaagaatga aaacagctta
    18421 tccaagggtt cccttccccc tcattgtaaa ggcgacagat ggcgacgtgg aagcaattaa
    18481 tcagattgtt aaacattatc gagggtatac ctcaaaacgt tcattacgtc gcatgacaga
    18541 cgaatatgga aattctcata tggttattga tgaaacccta cgtggcagaa tggaaacgaa
    18601 actcatctct aagattttat cgttcgaaat taaatagtcc cattttttcg tggaagcgtg
    18661 tcgaatcgta tccacgcttc ccgatttggg aggtttgtta ttccatcaaa gcatattgtg
    18721 tgttcagtag gttttgatag gctaacaagc caaacgttct ttgacaacca aataaaggca
    18781 atcgaatacg tttcggtaag aaaagagctg atgaactaac acgccatgac ctacaaaatt
    18841 agtgagcgat tcatgttagt aattcgaaag taatctttag caggattgct gccatgactt
    18901 tcttatcgtg ataatgatac tcccgtatag ccatagtccg agcgttagaa gcgtcacagg
    18961 caatgggtac ggctacatga gaaccatgca gaagtgaaat tcctgtgagc tttgctagag
    19021 ctgttcgatt gctaagatat tcttctcttg tcagtgtatt attataaaac tatttgatat
    19081 aattataggt gaatattgga gttttcctag cctataattg ggaaaggagt aataatgata
    19141 acggtcacaa aaaaggattt agttgagcta ggatatggaa cttctttttc agcagacatc
    19201 ataaaaaaag caaaagaatt gatgattaca aaagggcata tctactatca atctcgaaag
    19261 ctagatagag tccctcgtga agctgttgaa gaaattctag gaattcggtt ttcaaatgaa
    19321 aacaggtctg agtagttttt gcacagtaag gagtaaaaac tatggttaaa gacccaataa
    19381 aaaaagcaaa gaatggaacc tattattttc gtgcaaatct aggttatgat tctaaaggga
    19441 aaaaaattca aaaatatcgt agtggattca ctactaaaaa agaagcaaga gaagaatatt
    19501 cgaaactgct attgatgaaa ccagaagaat taagtgagaa taaggataaa atgacgttta
    19561 aacattatat ctttgaaatt ttccttccat ggtataagac acaggtaaag ctcagaacat
    19621 atgaaaatcg cttaccaact attaagaagc atttctctta ttttgataaa atggctgttt
    19681 ccgatattga gcctattgat gtgcaaaatt ggcaactaaa attatcaaag aagtgtaagt
    19741 cttcttatgt aagagctgtt caaggactat tttcagttgc tatggatcga gcaattgtcc
    19801 ttgggataac caccacaaat ccatctaaga ttattggcaa tgtgaaaaag caaaagtcta
    19861 aaattgaatt ttggacaaaa gaagaatttg aaaaagtaat ctctctaatt tacaaagaag
    19921 attactatca acacttttta tttatctctc tgtggttttt atttatgact ggaatgcgaa
    19981 ttggtgaagc tactgccatt caatgggaag atattgattt tgactcagga gtactaacca
    20041 tcaacaaaac actctattac aagaatcaat cgaattatag atttgttgaa ccaaaaacaa
    20101 aggcgagcgt tcgtcatatt gtattagata aatgcacatt aacttattta tccgaatgga
    20161 aggaagtaca acaaaatcta attcagacag actttgtaat gagctataac ggtattccta
    20221 cccaaaaaca tacactatca tatgctattg aacgctatgc taagctggct ggtatccatc
    20281 gaatcagaat ccatgggtta cggcattctc atgcttcctt gttaatcagt atgggagaaa
    20341 atccacttat cattaaagat cgtctaggac acgaagatat tgagacgact ttaggaacgt
    20401 atggacatct ttatcctaac agtaatttcg aggtagctaa caaattaaat ggagccattt
    20461 cctttaaaga agcaaaacaa aattatgata cttcaccaaa gaatcaattt actgtagact
    20521 acttgcgaaa caaagaaatg aaaaaagtgc aataaaagtg cagtaaaaag ataagaagag
    20581 ccagaaaccc ttggtataag aggatttcta gctctcctac aactattccc actccactgt
    20641 tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac
    20701 gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat
    20761 tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc
    20821 cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc
    20881 aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag
    20941 cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg
    21001 acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt
    21061 gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac
    21121 attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata
    21181 caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc
    21241 atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac
    21301 acctttcaac ttgtctaaga agcgatctct tgcatcgact ttgatgatgt tcaagccgaa
    21361 ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc
    21421 aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact
    21481 agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat
    21541 tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac
    21601 atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg
    21661 gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt
    21721 gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat
    21781 ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc
    21841 taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta
    21901 cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc
    21961 aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat
    22021 tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga
    22081 aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc
    22141 taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc
    22201 tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca
    22261 tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc
    22321 gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg
    22381 ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat
    22441 tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca
    22501 cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat
    22561 tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca
    22621 attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct
    22681 cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata tcataacttt
    22741 caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa
    22801 atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct
    22861 gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa
    22921 aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa
    22981 attctttcat ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct
    23041 gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg
    23101 cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg
    23161 gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa
    23221 acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa
    23281 agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat
    23341 gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac
    23401 ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac
    23461 agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc
    23521 tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg
    23581 accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa
    23641 tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga
    23701 tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag
    23761 caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc
    23821 tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag
    23881 tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg
    23941 atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag
    24001 agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt
    24061 aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt
    24121 acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg
    24181 aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt
    24241 acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata
    24301 tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa
    24361 gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata
    24421 agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg
    24481 tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga
    24541 atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca
    24601 agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata
    24661 ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa
    24721 tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg
    24781 cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg
    24841 aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag
    24901 atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg
    24961 gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga
    25021 atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag
    25081 aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat
    25141 tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg
    25201 atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta
    25261 ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag
    25321 catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt
    25381 ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg
    25441 aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga
    25501 gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt
    25561 gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat
    25621 tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca
    25681 ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt
    25741 cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga
    25801 caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat
    25861 tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa
    25921 aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag
    25981 ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac
    26041 tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac
    26101 gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc
    26161 tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg
    26221 aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct
    26281 tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc
    26341 aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat
    26401 cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca
    26461 aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg
    26521 tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa
    26581 aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga
    26641 agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat
    26701 gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt
    26761 tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa
    26821 agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt
    26881 ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga
    26941 gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa
    27001 agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa
    27061 tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt
    27121 tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag
    27181 agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac
    27241 atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac
    27301 tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat
    27361 ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc
    27421 tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa
    27481 caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat
    27541 tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt
    27601 gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta
    27661 tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc
    27721 tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt
    27781 acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat
    27841 gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata
    27901 aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg
    27961 ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt
    28021 catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg
    28081 gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag
    28141 atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag
    28201 attacatccc aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg
    28261 gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg
    28321 ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca
    28381 acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg
    28441 gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg
    28501 gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg
    28561 gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg
    28621 cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat
    28681 cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag
    28741 acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga
    28801 aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg
    28861 gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac
    28921 tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg
    28981 taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca
    29041 tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg
    29101 tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct
    29161 cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa
    29221 aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca
    29281 ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt
    29341 ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg
    29401 acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc
    29461 aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta
    29521 acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa
    29581 gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct
    29641 tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac
    29701 cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt
    29761 gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt
    29821 acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga
    29881 aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg
    29941 gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt
    30001 ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg
    30061 aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag
    30121 tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta
    30181 aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac
    30241 gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc
    30301 gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta
    30361 atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt
    30421 gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc
    30481 gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg
    30541 atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg
    30601 atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa
    30661 ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg
    30721 atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat
    30781 gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg
    30841 attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc
    30901 ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc
    30961 aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa
    31021 tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc
    31081 gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc
    31141 tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt
    31201 gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg ctttacatga
    31261 agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc
    31321 ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt
    31381 atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg
    31441 atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag
    31501 taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt
    31561 ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg
    31621 atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc
    31681 agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt
    31741 tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt
    31801 cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc
    31861 gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact
    31921 atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta
    31981 acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga
    32041 atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt
    32101 ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct
    32161 ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta
    32221 attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt
    32281 caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt
    32341 ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg
    32401 attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca
    32461 gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat
    32521 tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg
    32581 atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct
    32641 atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt
    32701 gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta
    32761 ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa
    32821 gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa
    32881 taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata
    32941 aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt
    33001 cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat
    33061 taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag
    33121 gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga
    33181 aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat
    33241 tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac
    33301 aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg
    33361 ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg
    33421 ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc
    33481 ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca
    33541 ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt
    33601 atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg
    33661 tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc
    33721 gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt
    33781 caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg
    33841 tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag
    33901 cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta
    33961 ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag
    34021 aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact
    34081 ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt
    34141 aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat
    34201 ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga
    34261 attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc
    34321 tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa
    34381 agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac
    34441 ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg
    34501 tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa
    34561 ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc
    34621 agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa
    34681 actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac
    34741 gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt
    34801 tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac
    34861 caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca
    34921 gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga
    34981 ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt
    35041 tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag
    35101 tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta
    35161 tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc catgaattga
    35221 tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag
    35281 acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg
    35341 aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag
    35401 catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag
    35461 attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag
    35521 gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat
    35581 taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac
    35641 gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag
    35701 aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc
    35761 tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac
    35821 caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaa
//
LOCUS       DACCCM010000253        23732 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.042, whole genome shotgun sequence.
ACCESSION   DACCCM010000253 DACCCM010000000
VERSION     DACCCM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.042"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            292..567
                     /locus_tag="IVK78_003006"
     CDS             292..567
                     /locus_tag="IVK78_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376372.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            580..2658
                     /locus_tag="IVK78_003007"
     CDS             580..2658
                     /locus_tag="IVK78_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6376373.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2818..4704
                     /locus_tag="IVK78_003008"
     CDS             2818..4704
                     /locus_tag="IVK78_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6376374.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4716..5663
                     /locus_tag="IVK78_003009"
     CDS             4716..5663
                     /locus_tag="IVK78_003009"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6376375.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5683..6210
                     /locus_tag="IVK78_003010"
     CDS             5683..6210
                     /locus_tag="IVK78_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6376376.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6273..6926)
                     /locus_tag="IVK78_003011"
     CDS             complement(6273..6926)
                     /locus_tag="IVK78_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6376377.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7058..7900
                     /locus_tag="IVK78_003012"
     CDS             7058..7900
                     /locus_tag="IVK78_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6376378.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8058..8954
                     /locus_tag="IVK78_003013"
     CDS             8058..8954
                     /locus_tag="IVK78_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6376379.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8957..9670
                     /locus_tag="IVK78_003014"
     CDS             8957..9670
                     /locus_tag="IVK78_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6376380.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9690..10208
                     /gene="msrA"
                     /locus_tag="IVK78_003015"
     CDS             9690..10208
                     /gene="msrA"
                     /locus_tag="IVK78_003015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6376381.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10205..10432
                     /locus_tag="IVK78_003016"
     CDS             10205..10432
                     /locus_tag="IVK78_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6376382.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10584..11135
                     /gene="lepB"
                     /locus_tag="IVK78_003017"
     CDS             10584..11135
                     /gene="lepB"
                     /locus_tag="IVK78_003017"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6376383.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11218..11760)
                     /locus_tag="IVK78_003018"
     CDS             complement(11218..11760)
                     /locus_tag="IVK78_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6376384.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11997..12395)
                     /locus_tag="IVK78_003019"
     CDS             complement(11997..12395)
                     /locus_tag="IVK78_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6376385.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12655..13515
                     /gene="ylqF"
                     /locus_tag="IVK78_003020"
     CDS             12655..13515
                     /gene="ylqF"
                     /locus_tag="IVK78_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6376386.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13508..14275
                     /locus_tag="IVK78_003021"
     CDS             13508..14275
                     /locus_tag="IVK78_003021"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6376387.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14331..15188
                     /gene="dprA"
                     /locus_tag="IVK78_003022"
     CDS             14331..15188
                     /gene="dprA"
                     /locus_tag="IVK78_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6376388.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15310..17388
                     /gene="topA"
                     /locus_tag="IVK78_003023"
     CDS             15310..17388
                     /gene="topA"
                     /locus_tag="IVK78_003023"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6376389.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17357..18733
                     /gene="trmFO"
                     /locus_tag="IVK78_003024"
     CDS             17357..18733
                     /gene="trmFO"
                     /locus_tag="IVK78_003024"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6376390.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18931..19836
                     /gene="xerC"
                     /locus_tag="IVK78_003025"
     CDS             18931..19836
                     /gene="xerC"
                     /locus_tag="IVK78_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6376391.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19873..20421
                     /gene="hslV"
                     /locus_tag="IVK78_003026"
     CDS             19873..20421
                     /gene="hslV"
                     /locus_tag="IVK78_003026"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6376392.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20435..21835
                     /gene="hslU"
                     /locus_tag="IVK78_003027"
     CDS             20435..21835
                     /gene="hslU"
                     /locus_tag="IVK78_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6376393.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21853..22644
                     /gene="codY"
                     /locus_tag="IVK78_003028"
     CDS             21853..22644
                     /gene="codY"
                     /locus_tag="IVK78_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6376394.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22726..23602
                     /locus_tag="IVK78_003029"
                     /pseudo
     CDS             22726..23602
                     /locus_tag="IVK78_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
BASE COUNT         8213 a         4092 c         4999 g         6428 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgattttt gtcgcagccc catttttttg
       61 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
      121 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
      181 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
      241 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
      301 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
      361 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
      421 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      481 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      541 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      601 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      661 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      721 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      781 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      841 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      901 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      961 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
     1021 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
     1081 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
     1141 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
     1201 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
     1261 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
     1321 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1381 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1441 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1501 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1561 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1621 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1681 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1741 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1801 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1861 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1921 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1981 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     2041 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     2101 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     2161 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     2221 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     2281 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     2341 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     2401 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     2461 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     2521 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     2581 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     2641 acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc
     2701 agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa
     2761 aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg
     2821 ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac
     2881 cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg
     2941 ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa
     3001 acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc
     3061 gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt
     3121 cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa
     3181 aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc
     3241 aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact
     3301 ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat
     3361 ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa
     3421 aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta
     3481 gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga
     3541 aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa
     3601 gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca
     3661 cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac
     3721 caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa
     3781 gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta
     3841 gatttattga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa
     3901 tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc
     3961 ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa
     4021 cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa
     4081 atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg
     4141 ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag
     4201 caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg
     4261 attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt
     4321 tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa
     4381 ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct
     4441 gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac
     4501 atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc
     4561 tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg
     4621 caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac
     4681 ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag
     4741 gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa
     4801 gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga
     4861 aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga
     4921 atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct
     4981 acatcaaaag tccagactat aatggaccag atatgactga ttttggccat agatgtttga
     5041 cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc
     5101 taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat
     5161 ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag
     5221 caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg
     5281 ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac
     5341 ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca
     5401 ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg
     5461 aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag
     5521 aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt
     5581 cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga
     5641 tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg
     5701 gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat
     5761 gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca
     5821 ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat
     5881 tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg
     5941 gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt
     6001 ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct
     6061 tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg
     6121 gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta
     6181 aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta
     6241 gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac
     6301 ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa
     6361 ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata
     6421 acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc
     6481 ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac
     6541 gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct
     6601 agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa
     6661 atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga
     6721 aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca
     6781 atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata
     6841 ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac
     6901 gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa
     6961 aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga
     7021 ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac
     7081 agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc
     7141 gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag
     7201 atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg
     7261 agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca
     7321 tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa
     7381 aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat
     7441 tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa
     7501 aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa
     7561 aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt
     7621 gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt
     7681 cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat
     7741 cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga
     7801 attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac
     7861 tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct
     7921 cagcctgaaa ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt
     7981 ttcttttttc ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga
     8041 atacaagagg tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat
     8101 tattaattgc gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac
     8161 taatgaaacc agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca
     8221 tagctatcgg agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg
     8281 ttccccttgt cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa
     8341 actatggtgt aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag
     8401 aaattcgaaa caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt
     8461 tgatgaaagt tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac
     8521 tgaaaaacta tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa
     8581 ccgcacctat ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca
     8641 ctgacatgca gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaaa
     8701 tgccgaactg ctactttatc ccaatcaatg atttgcttta tcaaggattg aatcaggaag
     8761 taggtgtcac agaagaagga acagatcaaa caactagcac gacagaaaat ctaaatggca
     8821 tcaaaaataa tgtgctctat gaagaagatc atttccatcc aaataattta ggttatcaat
     8881 tgatggcaaa tgcagtgaga gatcagctga tcgagacaca aaaccaatgg ttagagaagg
     8941 aggatgatca ataaacatga aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac
     9001 aaattactgg aaattcgcct tccttatcct tcttggggta gtacttggtt cgaccgtatt
     9061 tttaggcagc cgtatttttg ctaatcggga accagaactc ccagaaatac cagctcttac
     9121 ggaaagacaa ggagatcctg tcttaacgat caattcaaat aaagaaaaag tcaatcaaat
     9181 catcagcttt ttccttagtg agtatcaaaa agatagtgat attgaatata aattttattt
     9241 ggaaaatgaa gcactactca atggtacttt cgaagtacta ggttttccta tcgatttcta
     9301 tttatatttt gatccgtatg tgatggaaaa cggaaatgtc caactaaaag ctagaagtct
     9361 ttctatcgga acattgagtt tgccaatcaa agatgtgatg aatatgatca agagaaacta
     9421 taaattacca gattggatcg aagtcaatac agatgatctg actatcatgc ttaggttgga
     9481 tcaattccga atgcaaaacg ggatgtatat taaagcagat aaaatcgact tagtaaatga
     9541 tgacattcgg tttagtctat atttgccaaa agatacctct gatgcttcaa acaactcaaa
     9601 taccaacaaa actagcacca gcagtactga aagcaagaca aaagaaaata aaaaatcatc
     9661 ggatgagtag tattagaggg ggaatcataa tggaacgagc aatatttgca ggaggctgct
     9721 tctggtgtat gatccagcct ttcgacacgc ttcctggtat ccatacagtg atgtcaggat
     9781 atactggagg gcatgtacca gatccgactt atgaacaagt aaaaagccaa acgactggac
     9841 atacagaagc agtggagatt atctatgatc caacattgat ttcatatgaa caattagtag
     9901 aactttactg gcagcaaact gatccaactg acgctttcgg acaattcgag gatcgcggtg
     9961 acaattatcg cccggtgatc ttttatttga cagaagaaca gcggatcatt gctgaaaaaa
    10021 gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac
    10081 cagcccaacc attttatcta gcagaagaag accatcaaga gtattataaa aagcatcctg
    10141 aaaattttaa aagaaaccat gcacgccggg ctgcctttat cgaagaaaat tggaggaacg
    10201 aagggtgaat cgaagctttt ttcattattt aatgacactg cgaggcggtg gcacacatga
    10261 tgctttatct gttttcgcta cagaagccgg taaagacatc caatttccta agcacagctc
    10321 tagctacgaa gaaatttcta attatctgga actgaatgtc gactatttgc cgtccatgga
    10381 tatctttgac gaagcatggg aaaaatattt agaaaataat catgacatat aagagatagt
    10441 tttgtacttt atgatcaaaa aagttttttg tccaatagcc taattgaaag aattattttt
    10501 atttcagcta tctagtcgca tttttttgat atttttctta taataaagaa agaatgaaaa
    10561 aaagagggat ggattgatag agattgacaa aaaaacaacg ctatattaat cgcttctggc
    10621 ttgtattcaa atacttactt gtttctgtat tccttgcgtt catgttgcgg ggatttttat
    10681 tcatcccggt accagtggaa ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg
    10741 ttgtgatgga aaaattttcg gagatcagac gatttgatat tgttgttttt caattagcag
    10801 atggcacgat ttatatcaaa cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa
    10861 atgatcaatt gaagatcaat gggaaagtag ttaaggaacc ttatctaact aaaaacctga
    10921 aatcagatca tgcaaatgca tcctatacga cggactttac actacaagag ctaacgggac
    10981 aatccaagct tccggaagat aattattttg tacttggaga taatcgaaga gtttcaaaag
    11041 atagtcgttc gtttggcacg atcaataaaa ctgatattct aggtaaagca agatttgtct
    11101 attatccact tgatgaaata aaatggatcc agtgagaaac aataaagcgt gcagtctgtt
    11161 gagactgcac gctttattgt ttaatccaaa atcagtttac taccttagta cgtaaaatca
    11221 aacagctcgt ttaaacactt ttctagcctc taaagttttg ataaatgaat aaacaaccat
    11281 tatttgaata gggatttgga tcatattcct gatgaatcta cctagaaaaa ttactttgaa
    11341 atctgcacca aacaaaacag acatcagcca agaattgata aaaaggctga ctgggatcat
    11401 aatcaataat tgggcagcga tgacggaagg caggtttatc tttttatgat ataaaaataa
    11461 gccattaata aatccaccaa ggaaagccgt gataagaaaa gcagcttgaa acgttcctga
    11521 accaaatatc gtgaaattta aagtgttcga caacatttca agcaaaccag cccagatcgg
    11581 tccatataga taacccataa gcgccatact taaaaatccg aatgtgattt gaaaataagc
    11641 aggaccaatc gctatcttgt ataagatgac atcaagggct aataaaagag cagaggtcga
    11701 caacaatctt gcattcattt cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa
    11761 aatattctct cctttgtcag ttaataataa aaggatacca taaaatcacc aatgtgtcat
    11821 gacacattgg tgatttacat acactggcat ctaacaatta aaaaaagggt gagaagactg
    11881 cttttataaa ctgtaaaaaa gagattgtac gaataaaaaa aatagaggcg gagaatactt
    11941 atcgtatcat ccgcctttgt ttttttgtta taaaaaacgt cagattagtt atctgcttac
    12001 atcttcatcc atacgggttc taatgtattt ccgtctagat cttggacttc taatgaaaac
    12061 atttcttctt caggcattcc catatctact tcgtggaaag taccaccatt ttcttttgca
    12121 atttgagcaa atttttttac ttcatccgca ttttcaacac taaacgaaat caaagcacca
    12181 cttgtttttt tattatcagc tatttcttta tcttttataa attttttata aaaatcatga
    12241 gagagaagca ttatccaaaa agtgtcatcc cataccattg aaccagcttc ttcagtagaa
    12301 aattcttcat tttttttaaa acccagcttt tcataaaaag caacggaacg attgacatca
    12361 gcaacaggaa aattaacaaa aaccatacta ttcatttgtt tcgtctcctt tctgttttgt
    12421 tcgtctatca tctcttattt aggaaacgct gtcaaaaatt tagattcata tatagaaacc
    12481 aaaataaaaa tttgcatcat caaaaaagtg aagatgtgat agaatgagga ctggttcact
    12541 ttcacttaac taatagatgg aatttcttta taataaaaaa gtgaatttat attgaaaaag
    12601 aaaaaaacaa gtaaagccgg ggaatattcc ggtatgaaag gaatggagag aacaatgacc
    12661 atccaatggt ttccgggaca tatggcaaaa gcccgacgag aagtttctga aaaaatgaaa
    12721 tatgtggaca tcgtatttga attggtagac gcacgattac ctttatcttc tcgtaatcca
    12781 atgctagatc agatcattca gcaaaaacct cgccttgttt tattgaataa agcggatctg
    12841 gcagatccac aacagacaaa gctgtggcaa gaatattttc gtaaacaagg ccatcttgca
    12901 ttagctatca cggcacaaga aagtaaagga attaaaggtc tagtacctgc ggctaaagaa
    12961 gcattgaaag aaaaacgggc aagagatgct gctcggggga tcaagcctcg cgctattcgg
    13021 gcaatggtct taggtattcc aaatgttggg aaatctacgt tgatgaatcg actagtaggc
    13081 aagaaaattg cacagacagg aaataagcct ggcgtaacaa aaggtcagca gtggcttcgt
    13141 ttaggtaatg agttggaatt gttagacaca ccagggattt tatggcccaa atttgaagat
    13201 gaagaaatcg gcaaaaaact agcgctgaca ggcgcgatca aagaccagct tgtccattta
    13261 gatgatttag cgatttacgg attagacttt tttgcccgat attattcagg aagaatcagc
    13321 gatcgttatg gtttatcaat cgagcaagaa gaaaaattgg caccggaatt actgatggat
    13381 attacagcaa aacgtgggta taaagaagac tatgatcgtg gcagcgaatt agtcgtgcat
    13441 gatattcgtc aaggaaaact tggacgatac acactggatc gttgcgaaga aatggagcaa
    13501 aaaagtgatg actgaatcca tccaaagtat caaagaacga ttgaaaagca tacagagaat
    13561 agatgatcct acactagaat cattaagaaa tgatccacgc aaaggcgtgc agcaagcact
    13621 ggcttctttt gaacgacgat tgaaaaagga agcattaatt aaggaaaaat atgaagaaat
    13681 gttttgcttt gaaaagcaag ggtacgcaca aggtcaccaa tggattgccg gcattgatga
    13741 agtaggaaga ggaccattag ctggacctgt cgtggcagct gcagttatct tgccgaaaaa
    13801 tgagatcata ttagggctga atgactccaa acaattatca gagaaaaagc gagaacttct
    13861 ttatcaacaa gttcaagaaa aagccctggc aatcgggatc gggatcgttg atcagcagac
    13921 aatcgatgat gtgaatattt atgaagcaag taaattagcg atgatccaag cagtagaaaa
    13981 attgtccatt gtaccaagct atttgttgat cgatgcgatg accttggatt tgcctattca
    14041 gcaggaaaaa ataatcaaag gggatgcacg atctgtgtcg attgctgctg caagtatcat
    14101 tgctaaagta tatcgagacc aattgatgaa agaatatgac gtgctttatc ctggatatgg
    14161 tttctcgcat aatgcaggtt atggaacaaa ggaacactta gaaggacttg aacgacaagg
    14221 aattacgcca atccatcgta aaagttttgc acctgtaaaa aaatatttac aataaaaaac
    14281 agccgctttt acgtatctta ttagaaagag agacgaaagg cggttttatg atgtatcaaa
    14341 tagaagaaaa tttattgaaa cttgcttata caaaaggaat cagtagtcaa ggaaaatggg
    14401 cacttgctaa ctggatgatg cagtatcctt ataaagaaat cggatttgaa gacatcatta
    14461 agataggtaa aataaccaaa caccgggagc tgtttcgcaa aagttggcag gaaattcacg
    14521 ctaactgggg caaaatccaa tcgaaacagt cgtttatcac ttgctttgat cccaaatatc
    14581 cccctcagct gcttcacttg acctatccgc caattgtact gttttataat ggtgatctat
    14641 ctttactttc ttgtaacatg ttaagcgttg ttggtggaag acacacttct gctttagcca
    14701 aaaaaacagt ttattattta ctaaaaccag tgatagaagc tggatatgtg atcgtcagtg
    14761 gctgcgcgaa agggatagat acatacggtc accaagcagc aatctctcat ggtggacgga
    14821 caatcgcagt cattggaaca gggatagagc aagtctatcc tgctgaaaat cgtgacttgc
    14881 aagaggtgat tgcaaatgat catcttcttt tgagtgaata tcctcctcaa tccggcccga
    14941 aaaagcattg ttttccaatg agaaatcgga ttatcgcagc tttgtctaaa ggaacatgtg
    15001 tctttgaagc caagaaaaaa agcggctctc tgataacagc acagcaagca ttggatcttg
    15061 gaagaacagt tttttctgtt ccaggaaata ttttaacaga acattctacc ggatgtcatc
    15121 atttgatcca agacggtgca gtttgcacgg tttcgggtga agatatcttg gcagaattta
    15181 aagaataaca ccatactttt catttgcttt tcttgacaaa cgttttctga atggatatga
    15241 ttagctacga tttgtatgaa tcctctttta atagtaggaa cacgacaatg gaaaggagtc
    15301 cattcagtaa tggcgtataa atatttagtg attgtagaat cacctgcaaa agccaagaca
    15361 attgagaaat atcttggacg aaactataaa gtaatggcca gtgttggcca tataagagat
    15421 ttaccgaaaa gtaaaatggg tatcgatttt gaaaacaatt atgaacccca ttatatttct
    15481 atacgcggaa aaggcgatgt catcaaaagc ctgaaagccg cagcaaaaaa agctcaaaaa
    15541 gtttacttgg caagtgaccc ggatagagaa ggagaagcga ttgcttggca tttagcgtac
    15601 ctacttgggt tggatctgaa agaaaaaaat cgggtggtct tcaatgaaat cacaaaagac
    15661 gcagtcaaag cagcttttaa ggaaccaaga acgatcgatg tagatttagt agatgcacag
    15721 caagctcgtc gtaccttaga cagaatcgtt ggttattcga tcagtcctat tctctggcgt
    15781 aaggtcaaga aagggttaag tgcaggacgt gtccaatctg tcgctttaaa aattattatt
    15841 gaccgtgaaa aagagatccg agaatttgtt ccagaagaat attggagcat cgacggtaat
    15901 tttaaaaaag ctcgcaagaa attcaaagca aatttctggg gaatcgacgg taagaaaaag
    15961 aaattaccag atgcacaaag tgtaaaagaa gtcactgcta gaattgatgg aacagacttc
    16021 gaagttaaaa aagtagaaaa gaaagagcgc aaaagaaacc ctgcgaatcc tttcactaca
    16081 agtagcctgc aacaagaagc tgcccgtaag ttaaactttc gaacaagaaa aaccatgatg
    16141 gtagcacagc aattatatga aggaatcact ttgggtaaac aaggaacagt cggcttgatt
    16201 acatacatgc gtactgattc tacccggatt gccgatacag caaaagcaga ggccgcagca
    16261 tttattgaag atacgtatgg aaaagaattt tctagacatg atcaccgaaa gatcaaaaat
    16321 gctcaaggcg cacaagatgc ccatgaagct gtgcgtccaa cgagtgtgct tcgtacacca
    16381 gatgaactca aacaatattt agataaagat caattgaaac tttatacatt gatctggtca
    16441 cgttttgtgg ctagtcagat gacaccagct attttagata caatgaaagt gacattagaa
    16501 caaaatggtg tcatcttcat tgcgaacggt tcaaaaatca aattcaaagg attcatgcaa
    16561 gtgtatgtgg aaggtagaga tgacggcaaa gaagagaaag aaaatattct tccagaactt
    16621 gaagaaggcg atgtagtcca atctgttgat attgaaccaa aacaacattt tactcaacca
    16681 ccagcacgtt ttagtgaggc tacgttgatt cgtgcactag aagaaaacgg ggtagggcga
    16741 ccttccactt atgcaccaac acttgatacg atccaaagac gctactatgt aaaattgaca
    16801 caaaaaagat ttgagccgac tgaactagga gaaattgtca attcattaat ctgtgaattc
    16861 ttccctcaaa tcgtcgatat ccatttcaca gcagaaatgg aaggagattt ggataaaatt
    16921 gaagaaggca cggaagcgtg ggtcaaagtg gttgatcgtt tctataaacc gtttgaaaaa
    16981 gaactgacga atgcggaaga aaaaatcgaa aaaattcaaa tcaaagatga accagcagga
    17041 ttcgattgtg atgtctgtgg acatccgatg gtgataaaat tagggaaata tgggaagttt
    17101 tatgcttgca gtaattttcc tgattgtcgg aatacgaaac caatcgtaaa agaaatcggt
    17161 gtgacttgcc cagtatgtca tgaaggacaa gtcattgaac gaaaatctaa gaaaaacaga
    17221 ctattctatg gttgttcccg tttcccagaa tgtgaattca cttcttggga taaaccagtg
    17281 ggcagaaatt gtccaaaatg tgacggatat ctggtcgaga agaaagtcaa aggcggtaaa
    17341 caagtagtct gtgtaaatgg agattacgaa gaagacatcc aaaaataata gaaaagaggt
    17401 tctttcattg aaaatggtaa atgttgttgg agcagggctt gccggaagtg aagctgcttg
    17461 gcagatcgca caagcaggag tccctgtccg tttatatgaa atgcgtccag tcaaatcgac
    17521 tgaggcgcat cacacaagta attttgctga actagtatgt tccaattcat taagaggaaa
    17581 cagtttggct aatgcagtag ggttattgaa agaagaaatg cgccgattga attcggtagt
    17641 aatcacttcg gcagatgaaa cagcagtgcc agctggtggt gccttagctg ttgaccgcga
    17701 ttctttttct gaaacaatta cccgaaaagt caaagagcat ccattagtaa cagtgataaa
    17761 cgaagaaatt actgaaatac cggaaggaat caccgtagta gcaacaggac ctttgacttc
    17821 tcatccatta gcagaaagca tcaaagcatt caatggttca gatggttttt atttctatga
    17881 tgcagctgca ccaatcgtag acaaagctac gatcgatatg gataaagtat atttgaagtc
    17941 ccgctatgac aaaggagaag cagcttactt gaactgtccg atgacagaag aggaattcaa
    18001 agctttccat gaggcactaa tcaatgccga agtggcagaa cttaaatctt ttgagaaaga
    18061 aaaatatttt gaaggatgta tgccaattga agtaatggca cagcgaggat tcaaaacgat
    18121 gcttttcgga cctatgaaac cagttggatt ggaagatcct aaaactggca aacgtcctta
    18181 tgctgttatc cagctgcgtc aagacaatgc tgcagcttct ttatacaaca tcgtgggctt
    18241 tcagactcac ttgaaatggg gcgagcaaaa acgtgttttc cggatgattc ctgggttaga
    18301 aaatgctgaa ttcgttcgct atggtgtgat gcaccgaaac agctttatga attcgccaga
    18361 attgctgaaa ccgacttacc aatcaacgaa acgcgaagat ctgttctttg caggtcagat
    18421 gactggtgta gaaggctatg ttgaaagtgc agcgagcgga ctacttgctg gaatcaacgc
    18481 cgcacgctta gcaaaagaaa tggaaccagt cgagtttcca caagaaacag ctattggaag
    18541 tatggcttac tacattactc atgcggaagg aaaacatttc caaccgatga atgccaacta
    18601 tggtctgttt cctgaactgc cagagcgcat tcgagacaaa aaagcaagat atgaagcaat
    18661 tgcacaacga gctttaaaag ctaatgaggc aataaaaaca gaattagaaa aaactgcaat
    18721 cccaaatgag taaggaaact ccatgaagag ggtgagacgg atagtctcgc tctttttctt
    18781 gcgtttttct taagaatttc tcaaacaaaa tgtgaggaat tgcgccaatt gtgaacaaac
    18841 ttagaaatat tggttaaatt ctgacaattt atttgtcttt ttataaacgc tgtgctaaat
    18901 tattctctgg gacaaaagga aggtgagaaa atgacagaag ccacgtggga acaaaaattt
    18961 ttgcgttatt tgattgtcga aagaggatat tcagaaaaaa cgaaagaagc atatgaagaa
    19021 gacattctcc atttcaaaca ttttttggag gaatctggag acgctgatct gctgaaggcg
    19081 gaatatttta cggttcgtgt ctatttgagt gcattagctg atcatcgtcc tagctatagc
    19141 agaaattcta tcagtcgcaa gatagccagc ctacgttcgt tttatcagta tcttctcaaa
    19201 gaagaagtga taaaagaaaa tccattttct tacgttcatc tcaaaaagaa aaatttgaga
    19261 ctgccacgtt ttttttatga aaatgaaatg caagcacttt ttgacagcgt agcaggagat
    19321 agtcctttgg atttgaggaa ccgcgcttta ctggaagtcc tttacgggtc aggcatccgt
    19381 ttaagtgagt gtagcagttt gtcggtatca gacattgatt ttgattcaga ggtcatgttg
    19441 atccatggta aagggaacaa agagagatat gctcctctag gttcttttgc tcaagatgct
    19501 ctgcaagaat atttcaggga aggccgccag gtattgatga caaaatatca cgaagagcat
    19561 gatatcgttt ttgtgaatca tcatggaaaa ccaatcacgc ctactggaat tgaatatgtc
    19621 ttgaatcaaa tgatcaaaaa aagcagtttg gatagtgata tccatcctca catgctgcgc
    19681 catacatttg ctactcattt attaaataat ggggcagatt tgcggacagt ccaagaattg
    19741 ctaggacatg ccaatttatc gactacccag atctatgccc atgtaacaaa agaaagttta
    19801 caaaaaaact atcgttcttt ccatccaaga gcgtaaaaat aaggaaaaga acaaatttag
    19861 gaggaaacaa acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa
    19921 gacgggaaat ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg
    19981 aaaggcactg caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc
    20041 ggaagtgttg cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat
    20101 aacgggaatc tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct
    20161 atgcaaaaac ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca
    20221 ggtacaggag aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat
    20281 tttgcgttgg ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa
    20341 gaaattgcag aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat
    20401 attatcgtag aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag
    20461 acaaatcgta agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt
    20521 tgccgttgct ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga
    20581 tattacacca aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc
    20641 acgacgtttg gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac
    20701 tgaagtcggt tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc
    20761 gatccaaatc gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc
    20821 agaaaaacgt ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac
    20881 taatcaattt gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc
    20941 gcaagaagaa gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca
    21001 aaagggttta ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc
    21061 accaatgatg aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc
    21121 tttgacaccg aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt
    21181 gatcaaggaa gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa
    21241 attagcggaa gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc
    21301 acagcaatct ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga
    21361 aggttcacaa gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc
    21421 ttcaggagct ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt
    21481 cccgatacgg gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga
    21541 accaaataat gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt
    21601 tacgtttaca aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga
    21661 cacagataat atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct
    21721 attatttgaa gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga
    21781 tgacaaactt ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat
    21841 cggaggacga caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag
    21901 caaaagaata cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta
    21961 ggtggtattc ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat
    22021 aatgaacgtc acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt
    22081 cctgaatctt atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt
    22141 actagtgata ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg
    22201 actgtcgttc cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg
    22261 gaacaatcgt ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt
    22321 atgcagatcc tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct
    22381 gtccaaatgg caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt
    22441 gaagcattgg atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc
    22501 atcactcgtt ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa
    22561 tctcgttcgt taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac
    22621 gagttagcaa aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc
    22681 cagccttccc attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt
    22741 caaaatgacc agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa
    22801 tcaaaagaac atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat
    22861 gcaccggttt tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg
    22921 aaaacatatc aaatggggca acacggattt gctcgggata tggattttga agtcattgaa
    22981 catggacaag aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat
    23041 ccatttgatt ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat
    23101 taatcaagtg gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc
    23161 tttcaatgta cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc
    23221 attgaaatca agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac
    23281 gttaggtcag acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt
    23341 ggtttttgaa acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag
    23401 cgtaacttta agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga
    23461 agcgcctttc gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg
    23521 aaacttgatg gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa
    23581 atattccatc acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta
    23641 ccaaggtgtg taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtggc
    23701 tctttgtcaa taaggactga tgagttgtgc aa
//
LOCUS       DACCCM010000254        20985 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.052, whole genome shotgun sequence.
ACCESSION   DACCCM010000254 DACCCM010000000
VERSION     DACCCM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.052"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(134..577)
                     /locus_tag="IVK78_003030"
     CDS             complement(134..577)
                     /locus_tag="IVK78_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6376395.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IVK78_003031"
     CDS             complement(795..2135)
                     /locus_tag="IVK78_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6376396.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IVK78_003032"
     CDS             2404..3300
                     /locus_tag="IVK78_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6376397.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IVK78_003033"
     CDS             complement(3450..4391)
                     /locus_tag="IVK78_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6376398.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IVK78_003034"
     CDS             4517..5638
                     /locus_tag="IVK78_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6376399.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IVK78_003035"
     CDS             5896..7398
                     /locus_tag="IVK78_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6376400.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IVK78_003036"
     CDS             7465..7992
                     /locus_tag="IVK78_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6376401.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IVK78_003037"
     CDS             8203..9264
                     /locus_tag="IVK78_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6376402.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IVK78_003038"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IVK78_003038"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6376403.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IVK78_003039"
     CDS             10177..12159
                     /locus_tag="IVK78_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6376404.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IVK78_003040"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IVK78_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6376405.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IVK78_003041"
     CDS             12881..14305
                     /locus_tag="IVK78_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6376406.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IVK78_003042"
     CDS             complement(14486..15259)
                     /locus_tag="IVK78_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6376407.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IVK78_003043"
     CDS             complement(15256..16161)
                     /locus_tag="IVK78_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6376408.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IVK78_003044"
     CDS             complement(16315..16635)
                     /locus_tag="IVK78_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6376409.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IVK78_003045"
     CDS             17109..17795
                     /locus_tag="IVK78_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6376410.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IVK78_003046"
     CDS             17797..18741
                     /locus_tag="IVK78_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6376411.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IVK78_003047"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IVK78_003047"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6376412.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IVK78_003048"
     CDS             19663..20940
                     /locus_tag="IVK78_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6376413.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcgt tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcacta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACCCM010000255        16775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.064, whole genome shotgun sequence.
ACCESSION   DACCCM010000255 DACCCM010000000
VERSION     DACCCM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.064"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            366..1229
                     /locus_tag="IVK78_003049"
     CDS             366..1229
                     /locus_tag="IVK78_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6376414.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1329..1811
                     /locus_tag="IVK78_003050"
     CDS             1329..1811
                     /locus_tag="IVK78_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6376415.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2074..2541)
                     /locus_tag="IVK78_003051"
     CDS             complement(2074..2541)
                     /locus_tag="IVK78_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6376416.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2664..3515
                     /locus_tag="IVK78_003052"
     CDS             2664..3515
                     /locus_tag="IVK78_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6376417.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3766..5724)
                     /locus_tag="IVK78_003053"
     CDS             complement(3766..5724)
                     /locus_tag="IVK78_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6376418.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5721..6593)
                     /locus_tag="IVK78_003054"
     CDS             complement(5721..6593)
                     /locus_tag="IVK78_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6376419.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(6935..7807)
                     /locus_tag="IVK78_003055"
     CDS             complement(6935..7807)
                     /locus_tag="IVK78_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6376420.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            7935..8504
                     /locus_tag="IVK78_003056"
     CDS             7935..8504
                     /locus_tag="IVK78_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6376421.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8571..9308
                     /locus_tag="IVK78_003057"
     CDS             8571..9308
                     /locus_tag="IVK78_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6376422.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9539..10807
                     /locus_tag="IVK78_003058"
     CDS             9539..10807
                     /locus_tag="IVK78_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6376423.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11294..11758
                     /locus_tag="IVK78_003059"
     CDS             11294..11758
                     /locus_tag="IVK78_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6376424.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11742..12371
                     /locus_tag="IVK78_003060"
     CDS             11742..12371
                     /locus_tag="IVK78_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6376425.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12725..14203
                     /gene="msr"
                     /locus_tag="IVK78_003061"
     CDS             12725..14203
                     /gene="msr"
                     /locus_tag="IVK78_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6376426.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14250..14696
                     /locus_tag="IVK78_003062"
     CDS             14250..14696
                     /locus_tag="IVK78_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6376427.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14895..15410)
                     /locus_tag="IVK78_003063"
     CDS             complement(14895..15410)
                     /locus_tag="IVK78_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6376428.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15527..16729
                     /locus_tag="IVK78_003064"
     CDS             15527..16729
                     /locus_tag="IVK78_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6376429.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         5300 a         2989 c         3482 g         5004 t
ORIGIN      
        1 gatgaggttg aacttttaca caagattgtg gacttgactg caaagaaaaa tccaagcagc
       61 gttctgatca gcagtttgaa caacaatttg ctttgtacag taggtagtaa gcggattaac
      121 taagggaata aaagatattt ttaccgcttt gtttacttag aatctgtctt ccccaaaatt
      181 tatttttcta aaggtaaaac atagataggc agataatacc aattatccgt tacgaaacgc
      241 tgcttgagaa ttttctttag atccatacaa cattttctac atctgactaa attggttcct
      301 gattcatcaa gtaattgact ttctttgata atatgataaa aaggcaatca aggaggaaga
      361 taaatatgct taaagatctg aaccaattga tcgattacat cgaagaacat ttgacagaag
      421 gactttccta taaaataata gccgaaaata caggaatctc tgaatatcaa ttaaaacgca
      481 tctttacgtt tattgcaggg atgtcgctaa tggactatat caaaaaccgt agattggctt
      541 tggctaatcg cgatcttcta gatggatcaa gagtgacaga aacggcgttt agatatggat
      601 atgaaacagt cgaaggtttt tcaagagctt ttcgtgattg gtcaggatat ctgccttcag
      661 aaatcagcag atacggcata caaaaatctt ttccacgctt gacatttttt attgatgtca
      721 gaggaggaaa atcgatggaa tttcgtatcg aaaagaaaga acagttttat gtggcaggtg
      781 tggcgaaaaa agttcctgtc aaatttgaag gagtcaatca agaaatacaa aagttagcgc
      841 agacgatcac ggagactcaa agggaagaga tgcataagtt aggagattta tatccaaatc
      901 aagtaatcaa cgtttcttat ggatttgatg gagaccgctt ggaagaaaag ggcagtttga
      961 tgcacatgat tggctttgct acgacaaaag aaaatccgta cgctgattta gaaatgcttg
     1021 ctatagaaga acacacttgg gccatttttc ctaataaagg gccatttccc caaactttac
     1081 aagatacttg ggggaaaatc tatgcagaat ggcttccttc ttcagattat gggttagtcg
     1141 aagctcccga gatctctttt acaaaatggg gagaggattt cagtaatgtt tacagtgaga
     1201 tttggattgc agtgaaagca aaaaaatagt taaaaaataa aaatatccct agaaaaaaga
     1261 gcgtaatcgt gtcatttccc tttaatttta tgtaagatga acttaatata atagaaaggg
     1321 tgggagaaat ggcaaagtat aggatcgaag aaaaaccaga ttttacggtg acgggtattg
     1381 gtgtggaatt gaagagtagc aatacggatt ttgtcggttt ggctgcagag aaacaagcat
     1441 ttgagcaaaa agttatcggt aatggaacaa tagatgcatt gaaacaagct gctgaaaatc
     1501 attatttctt tacagtcaac gaaccctata acgggaaaat gatgtactat gtcggtgttc
     1561 aaacgagtca ggatgttcca aacactacgc gaatcatccc attcccaaaa ggaacgtatc
     1621 ttgttgtaag cgctttagca gaatcgcctg aagaattaag aaatgatttg acgaatacgg
     1681 cctttgggca tgttttaaga gaagtagaag attacgccta tgttgggggt ccaaatgctg
     1741 tagtcgaaat gggagttacc aacgggaaat atatgggaga aatctggata ccggtagtca
     1801 ataaaccatg ataatataga ggtcgtgaaa gtagcgcttt gacctgagca ttagagagga
     1861 aaaatgggaa atagctttcc atattttgca tttttcagaa ctaatgccga gggtcagcta
     1921 cttgaacacc gtttatcaag acgtcatgac ttcagcgctt tgacctgagc attagagagg
     1981 aaaaaggggc atgacaaggc ttttgtcccc cggagaataa aagaatttat gtagacgatt
     2041 ccgatcagaa aaatgcaggc acagtagaga gaattataaa aatctctcta cgaggcctgt
     2101 gttttctggt cttttttctt tttccaaaac ggctatttta tgtatcagac tatcaagtgt
     2161 gatcttagcc atttcttctt ctgccttttt ctgcagccga gcgtagcttt cttctaaagc
     2221 ttcttggata ttcgcaccaa cgacgcaatt tggatttgtt tttggatcga cttggataag
     2281 ctgtgtatta ccttcgatgc tgcgatagac atctagtaaa gaaatctgat ctggactttt
     2341 ggcaagggaa ggctgcggtt ttccggtttt tgtaaagatc aaaccggatt ttttgagctg
     2401 actcatgatt ttacgtacat ttgctgcatt tgtttcgata ctactggcaa tcatctcact
     2461 agaaagtgat cgggtatctt gaaacatttc aatataggca agtacatgga ttgcatcact
     2521 aaattgcaga gaatacttca tcagttgtca tcctttcatt ttttttgctt gacttatctt
     2581 accacactct ttatgataaa ggtgtaattt ataaatgtac agctgtaaat cttaacaaat
     2641 aaaaagaata gggagtggca aatatgcatt atttagtaac aggagcaact ggaggttttg
     2701 gtggttatgc gctggaattc ttaaaaaaga tggttccaca aaatgaaatc tatgtactag
     2761 tacgtagtga agaaaaagga gaagcactga aagaagctgg atttaatata cgtattggtg
     2821 attatgatga tttagactca atgaaagaag cgttgaaagg aatagatcga ttgctttttg
     2881 tctctggcgt tccaggaaat cgtcaagccg aacataagaa tgtagtgaag gcagcacaag
     2941 cagctggggt atcttatatc gcttatacaa gttttgcaga tgccgatcac tcgatcagtg
     3001 tattagcgcc agatcaccaa ttcactgaaa aaatgattaa ggattcaggt atttcccata
     3061 catttttacg gaataattgg tatgtagaga atgaattacc tatcattggg caggctttaa
     3121 aaactggtca attcgtttat gctgcaggaa atggaaaaac aggttgggca ttgaaacgtg
     3181 aatatgcaga agctgcagca aaagtactag tagaaagtga ctctccagaa atcttagaat
     3241 tatccggcaa tctaactgac tatgaagaat tagccaaggc attagagcgt gcgacaggta
     3301 aagagttaga aattattgaa gcaagtgatg cagcgtttgt cgaaaatcta aaagaagccg
     3361 gattccctca agaagcagct gacatgtttt tagcgatcca acatgatatt aaaaaggatc
     3421 agttagctat ttcctctgat gatttagaaa aagtattggg aaaaccatta acttctcttg
     3481 aagcagcatt aaaagaaata ttggatcagg aataatgtat aaagaggttg tgaaaaagcc
     3541 gattagtttt gagtatgaag aaaaattttg tgaaaaagct gttctgcatc aagtaataag
     3601 aagagtcaaa taaaaaatag ctcttcatat gtttcgttta gctggactta ttaccgcgga
     3661 gttggcagga gaacgccgtt tttattcgga aaaaggagct gagacaagat ttttgtctca
     3721 gctcctgttt gtttatcgat aaaaaagtct ggggaagacg atggctcagg ctttgttgga
     3781 aacagagccg attttatcat ttgcttttac atcacgtgcg ggttcgattt ctaagctttg
     3841 gactttgtcg ccatttgtga atacaacgac catatccgtg tttttgcctg cagattgaat
     3901 agcggcaaga tcaactaaag caatcaattg acctcttgcg actttttgac cttcttctac
     3961 atataaagtg aatggttcgc cttttaagtc aacagtgttg atgcccatat gaagcaagac
     4021 ttcgatacca gcatctgttt ggattccgac agcatgtttc gttgggaaaa tgtttgtgat
     4081 cgtaccagcg attggagaga agatttctcc attttctggt aatacagcat agccgtcacc
     4141 catcattttt tccgcgaata catcatctga aacttcagtg attggaatca gtttcccatt
     4201 tgcgatagcg taaatatctt gttcgatcgt ttcaccggca aatgtttctg ttttaaccac
     4261 ggtgtttgtt tgtgcaggtc gtgctgcagc accaccatgt agttttgcca tctcatctgc
     4321 aacgaattgt acttctgtac ctacgatgac ttgcgcattt tttccgtcga tgatcttcat
     4381 accaggtacg ccagtggctt tgattttatt ttgatcgatc aggcctgtat ctttgacttg
     4441 caaacgaagg cgtgtagtac agttatcgat cgaagttaca ttgtcggctc cgcctaatgc
     4501 agcgtagatt ttgcttgctt gtgccgcata tttattgtct gaattagtcg ttgcagtatc
     4561 gtcatctgaa agatctgctt cctcgcggcc aggagtcatc agatggaatt ttttgatagc
     4621 aaagttaaag ccaaagtagt aaatcactgc tgttactaat ccttggacga tcaacatcaa
     4681 tggctgattc gcaatcggat ttttcaaaga taggaagtaa tcgacaaatc cggcactaaa
     4741 ggcaaatcct gctgtccaat ggaagaatgc actcactgct aaagaaatcc cagtgaaaat
     4801 tgcatgcaag acatataatg gccaagcaac gaacataaat gaaaattcaa gtggttctgt
     4861 cacaccagta aagaatgaag caaaagctgc tgctagcatc aaggaagctg tttttgcttt
     4921 gttttctgga cgtgcatttc tgtaaatagc atatgctcct gctggaagcc cgaacatcat
     4981 gattgggaaa aatccagctt gatacattcc tgttacgcct tttgttcctt cagaagccaa
     5041 aaatttacca atatcattga ttcctgctac gtcaaaccag aacacggagt tcattgcttg
     5101 atgcaatcct gtagggatca gtaaacggtt gaagaagcca taaagacctg caccacccca
     5161 gcctaaactc aaaatgaatt tagcaaaaga aactaaagca gagtaaaccg gaggccatac
     5221 gagcagtagc actgcactga tcagcaacat gacaaaggca gccaagattg gcactagacg
     5281 cttgccgcta aagaaagata aagccatcgg taattttgct tcgtggaaac gattataaag
     5341 agctgccgca atcaatccgg caataatacc aataaatacg ttactgatct gagcaaaaga
     5401 tggatcaact gattctggtt tgatattcaa taaagtagct actgaatcag tttttaaaac
     5461 atttactgga acttcaaatg cgaccaaacc tgccaaggca gcagcacctg atttgtccac
     5521 tgacatccct agtgctaacc caacggcaaa cagcaaaggt aattgaccta aaactgcgtt
     5581 accgccagct tgtaaaaaat gagcgacaat gtcgttacta taagatgccc accagtttcc
     5641 gatccctact agtaaagcag cagcaggtaa gaccgcaact ggaagttgaa gggaacgtcc
     5701 cattcgttgt agatatgctt tcaatttttt tcctcctcat gattgttaac atttgtttgt
     5761 acgattgcgc ttcttaaacg ctcgatatgg atggccaagt aacccagttc ttctttcgtg
     5821 atcgcgagct ggtattcttt ggtcagcaac acacggattt ttttagaaat cttatagctt
     5881 tctgggtatt ttcgtttgac catttctacg atttccgtgt ctaaaacagc atattgttgc
     5941 gcatgggttc gttgtagaag tagtcgtaaa tgattcacca gtcttgaata attcagtgaa
     6001 aatggttctg aatggatatc tgtttccaat tctgattcca ctaaatggat gatgtcagat
     6061 acaatcgtca cttcacggat actgcgatgg ttattgttcc gaccgcttcg tccgctgtga
     6121 atatggattg cgatatatcc tgcttcgtca taagtatatg gaatcccaag ctcttttgtc
     6181 agatactcta ctgcccattg agcaatactg aattcatcac tgtaaagcac ttcgatttct
     6241 cttaaaagtt tgttgcggat gacgataccg ttattaatgt tctccgcaga aaaagcaatg
     6301 tggtcggtca aagcgatcaa tacatgttca ttcaactttt ccatcaaaac agtttccgca
     6361 tgatcaatga tcgtttctgc tgcaaataaa aagcgttcat caatctgatt aagtaaattt
     6421 tgcaacttga tttgaccttc tggttccatg acaaatatcc gttcgacatc acgatcaaaa
     6481 atcaaatcat ttctttttct gtcaaatcca atcccttttc caatagctac tttttcctgt
     6541 ccaccgtcat ctaccaatac tgcattttga ttaaagatct ttttgatttt catgcgcgca
     6601 ccctttttga agatttggtt ttcacgtttg ctagaccagt tgattgcggc caaacaaaaa
     6661 tagacacgaa aaacctccta gggaaaccat gttcctcaga gatttttcat gcctagtatg
     6721 atctagtcac atgaaacgaa ttattgttaa cgtaatcaat tttatataga cagttatgtt
     6781 ttgtcaattc tttttttata attatagtaa tttactggaa ttatcagaaa taaaaggaag
     6841 aaataaatta agaaacttat gttatttaat cgttggatct tttgcttatc ttattcgttg
     6901 ttaaaaagct gaaagcgaag gaaaaaaact ctacttaact gtatttctcc gttaggacat
     6961 ctaaaaattt acgagcagca ggagaaagag gctggtcttt tttccaaatg agattcagtg
     7021 aagcttgtaa tggcggatcg agtgggatga atattttttc cgtcccataa gtattgatga
     7081 tcccatctag agctaatgca ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca
     7141 ataagttata tgttccgacg atcgataact gatctatgct gaaacctagc cattcagcta
     7201 gttgactgtc cactgacgtt tgccgggaaa tgatcaaagg aacagttttc agttcctgcg
     7261 gatggatgac ggtttgcttt gctaaaggat gatttcgatg tatcaaaagt ccccaacgat
     7321 cttgggctgg cagtcgaaat gactggtatt tttgtttgtt tgtcggttcg attacgagtc
     7381 caaaatcaag taatccgcta tccagtttgt ctatgatatc atctgcattc ccgctatata
     7441 aatgaaactg gatatcagga tgcgccttga tcaacgaatg aaggctatca gaaatgaaag
     7501 agattgcttt agtttcgcct ccgccaatgt agatttctcc agagatgatt gcgtcatttg
     7561 ttaaattgtt cgccgttttt tctaccaacg acaagatttc ttttcctttt gaataaagat
     7621 atcgaccaga atccgtcaac tgtatctctc gggagccccg agtgaaaagt gtcacaccaa
     7681 gttcttcttc taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt
     7741 ttgctgctcg gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa
     7801 tatccatttt ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat
     7861 agttaagtat gttttataag tattagacat gataagccgt ccgttataaa gtaaggataa
     7921 aaggagggag caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag
     7981 taacggatcc gctctttgaa gtgatccatc agatccaagc tgaaaacgaa gagcctttag
     8041 cagcgctgaa tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca
     8101 ttagtgacaa agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca
     8161 aacatattag tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag
     8221 gtggcatctg tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga
     8281 accatctaac cgatccaaaa gaacgaagag gcttgtcagt aaagccaatc cacatcaaga
     8341 aaaatgcttg gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg
     8401 ccattgttgc agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag
     8461 gaatccctgc caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata
     8521 agagatagtg agtataagca agagattttt caaaacagga ggagaataga atgaatgatt
     8581 tatcagaaaa agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga
     8641 agctattagc agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa
     8701 aagcaataaa agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag
     8761 attttgcaca agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg
     8821 tattgtacaa taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg
     8881 aatggcaaaa tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac
     8941 ttccaatcat ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac
     9001 atgttgttta tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca
     9061 tggaaggatt acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag
     9121 gagctgttca aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc
     9181 atgaagcgca aaaagaatgg ggattgacct ccgaagatat agcttctgct gtagcatttg
     9241 cgatagaaac gccagaccgg atgtctgtca gtaacatgat tatccgtccg acgacacagg
     9301 aagtataatc cataacggat aatatccaaa gagaattact tgttaaatag ttattcaaac
     9361 gaccatcaat ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgtag
     9421 aaaaagcaaa ttcatcaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt
     9481 tactttttta gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat
     9541 gaaagcaaag aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac
     9601 aacagtgcta gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga
     9661 aacaggaatt gaaaacgata cagagagtac tttgcagtca gctactgtct cagaaaatac
     9721 aatttcaacc gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac
     9781 atcagcagaa gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga
     9841 tgggcaaaca ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg
     9901 ctatgctttt tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc
     9961 ctcaactgca caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca
    10021 agccatcgtt gaaagtggct gggggaacag tactttggct tctgccccta attacaatct
    10081 atttgggatt aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt
    10141 gaatgggcaa tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc
    10201 attgcaagat aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta
    10261 ttccggtgca tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg
    10321 tcgttatgcc actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa
    10381 tctgacacag tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg
    10441 tcatacagga gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt
    10501 aggctgggaa tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg
    10561 tttatacaat ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc
    10621 tttaacaaaa atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc
    10681 aatttatcga ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc
    10741 taaggaaaga gattggctaa caccgatggg ctggagatat gaaggaatag gattttatgg
    10801 ttattaaaaa gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa
    10861 aaacagattt tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa
    10921 caccgtttac tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta
    10981 agcatactga tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat
    11041 ttttggcggt gtaaaatttt ctactgcaga agagctatct tctatagttt gataagaatc
    11101 aataatcttg gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa
    11161 atttcttttc tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt
    11221 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
    11281 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
    11341 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
    11401 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
    11461 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
    11521 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
    11581 ccagaagaaa atcgagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
    11641 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
    11701 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
    11761 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
    11821 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
    11881 tgcaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
    11941 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
    12001 atatgcaatg ttacgaatat ggcgaagaaa tctataacca agcttacgaa tcagggaaga
    12061 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
    12121 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
    12181 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc
    12241 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
    12301 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
    12361 cgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
    12421 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
    12481 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
    12541 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
    12601 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
    12661 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
    12721 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
    12781 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
    12841 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
    12901 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
    12961 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
    13021 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
    13081 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
    13141 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
    13201 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
    13261 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
    13321 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
    13381 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
    13441 accggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
    13501 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
    13561 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
    13621 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
    13681 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
    13741 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
    13801 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
    13861 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
    13921 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
    13981 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
    14041 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
    14101 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
    14161 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
    14221 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
    14281 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
    14341 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
    14401 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
    14461 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
    14521 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
    14581 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
    14641 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
    14701 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
    14761 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
    14821 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
    14881 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
    14941 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
    15001 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
    15061 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
    15121 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
    15181 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
    15241 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
    15301 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
    15361 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
    15421 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
    15481 tttacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
    15541 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
    15601 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
    15661 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
    15721 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
    15781 tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg
    15841 attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc
    15901 tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa
    15961 agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg
    16021 tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt
    16081 ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt
    16141 aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat
    16201 cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat
    16261 tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat
    16321 tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt
    16381 agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc
    16441 agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact
    16501 gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta
    16561 tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga
    16621 acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg
    16681 ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg
    16741 ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DACCCM010000256         7959 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.109, whole genome shotgun sequence.
ACCESSION   DACCCM010000256 DACCCM010000000
VERSION     DACCCM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.109"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            128..1564
                     /locus_tag="IVK78_003065"
     CDS             128..1564
                     /locus_tag="IVK78_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6376430.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IVK78_003066"
     CDS             1753..4200
                     /locus_tag="IVK78_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6376431.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IVK78_003067"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IVK78_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6376432.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IVK78_003068"
     CDS             complement(5690..6793)
                     /locus_tag="IVK78_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6376433.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVK78_003069"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVK78_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6376434.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACCCM010000257         4614 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.136, whole genome shotgun sequence.
ACCESSION   DACCCM010000257 DACCCM010000000
VERSION     DACCCM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.136"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            107..1024
                     /gene="vanY"
                     /locus_tag="IVK78_003070"
     CDS             107..1024
                     /gene="vanY"
                     /locus_tag="IVK78_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP6376435.1"
                     /translation="MKKLFFLLLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKE
                     HLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYG
                     LLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPA
                     GYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWH
                     IRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPT
                     NLRYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1177..1662
                     /gene="vanZ"
                     /locus_tag="IVK78_003071"
     CDS             1177..1662
                     /gene="vanZ"
                     /locus_tag="IVK78_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6376436.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1949..2278)
                     /locus_tag="IVK78_003072"
     CDS             complement(1949..2278)
                     /locus_tag="IVK78_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6376437.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2583..2912
                     /locus_tag="IVK78_003073"
     CDS             2583..2912
                     /locus_tag="IVK78_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6376438.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2902..3189
                     /locus_tag="IVK78_003074"
     CDS             2902..3189
                     /locus_tag="IVK78_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6376439.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3468..4031)
                     /locus_tag="IVK78_003075"
     CDS             complement(3468..4031)
                     /locus_tag="IVK78_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6376440.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1626 a          690 c          777 g         1521 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc cgtttaaggg gaagattcta
       61 gaaatatttc atacttccaa ctatatagtt aaggaggaga ctgaaaatga agaagttgtt
      121 ttttttattg ttattgttat tgttattctt aatatactta ggttatgact acgttaatga
      181 agcactgttt tctcaggaaa aagtcgaatt tcaaaattat gatcaaaatc ccaaagaaca
      241 tttagaaaat agtgggactt ctgaaaatac ccaagagaaa acaattacag aagaacaggt
      301 ttatcaagga aatctgctat taatcaatag taaatatcct gttcgccaag aaagtgtgaa
      361 gtcagatatc gtgaatttat ctaaacatga cgaattaata aatggatacg ggttgcttga
      421 tagtaatatt tatatgtcaa aagaaatagc acaaaaattt tcagagatgg tcaatgatgc
      481 tgtaaagggt ggcgttagtc attttattat taatagtggc tatcgagact ttgatgagca
      541 aagtgtgctt taccaagaaa tgggggctga gtatgcctta ccagcaggtt atagtgagca
      601 taattcaggt ttatcactag atgtaggatc aagcttgacg aaaatggaac gagcccctga
      661 aggaaagtgg atagaagaaa atgcttggaa atacgggttc attttacgtt atccagagga
      721 caaaacagag ttaacaggaa ttcaatatga accatggcat attcgctatg ttggtttacc
      781 acatagtgcg attatgaaag aaaagaattt cgttctcgag gaatatatgg attacctaaa
      841 agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa tatgagatct tttattatcc
      901 tgttactaaa aataccacca ttcatgtgcc gactaatctt cgttatgaga tatcaggaaa
      961 caatatagac ggtgtaattg tgacagtgtt tcccggatca acacatacta attcaaggag
     1021 gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag cattattgta ctagcacttt
     1081 tggggtaacg ttagcttttt aatttaaaac ccacgttaac taggacattg ctatactaat
     1141 gatacaactt aaacaaaaga attagaggaa attatattgg gaaaaatatt atctagagga
     1201 ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt acaatacaat
     1261 attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt tactgctact
     1321 gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg cttgcttttg
     1381 aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact ggttttaagt
     1441 cttacttttg aaataattca atttatcttc gctattggag cgacagacat aacagatgta
     1501 attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag caataagcat
     1561 atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt gctcgtatta
     1621 ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta aatcaagcaa
     1681 tctgatcttt catacacata aagatattga atgaattgga ttagatggaa aacgggatgt
     1741 ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt aaaaagctta
     1801 cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag gaattggtct
     1861 ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc cccaaactaa
     1921 gatttcatag atagattttc tgataacttt aaaaaacacg aatagcggaa aggatgtctt
     1981 cattcgctaa agagtaataa actacttttc catcttgacg tgattttgcc attccatgtt
     2041 tttttaaaaa acgtaaatgg tgtgacgttg tagcgacact agcagtaagt atttctgcta
     2101 aatcacagac gcacatttcc ttataggttt ctaaagcata gaaaatttta attctagaaa
     2161 aatcgctgaa acatttccct aacacaagta agtttgaaaa atctttttga tccaatttat
     2221 tttttattcc atttactttt tctttatgta cgacagtcac ttggcaaact tttttcattc
     2281 atctcacctc acattcaaac gattatttga atgaagtata cctatttgat cactcttttg
     2341 tcaatctacc ttaattccaa tacactatta taagcaattt catgtattga agtaaactta
     2401 cttgcaagtg cgtttttgat tccaagttaa aaacatatta ttattctaca taatttccta
     2461 atcaaataaa actcctttga aaattgttct aaaggagttt tattattttg tatttctact
     2521 aaaaatagcg tacaatgtaa ttactaagta attacgaaat gtaaaataat ggaggaaata
     2581 atatgattac aaaaacaaga aaacaaggaa attctattat gttaacggtc cctaaagaat
     2641 tcgatgtgcc caatggtgtt gaagtagaag ctaagcttgt agagaatggt atcctttatg
     2701 agtttgtaga gcctaaaaaa gaattttttg attttagtga agatattttg gctgatattc
     2761 tttcagaagg ttatagcaaa caagagatct taaaagaatt tagaaataga aaaaatgcgt
     2821 taacttctgc atttaaatct attgctgaag atactgttgc aaattctaaa ccgatgacaa
     2881 aagaggagtt ggcagcagaa attggcttat gagattctac cttctaaaca cgtaataaag
     2941 tatctaaaaa aattaaaaga gaaaacatta aaagaacaat ttttaacgat aatttatgat
     3001 gagatagcag ttcgtcctca ttcgggagaa caaaaaacgg gtgatttatc aggaatttgg
     3061 gctattggat tcaagtatgc aggtacgaca tatcgcgttg catatgaaat taaagataat
     3121 gcagtgatac ctatcttact atgtggaacg catgaaaatt tttatgaaca attaaaaaag
     3181 attaggtaag aacccaatgt ttcaacttga gaggatagac gacactttaa aagataattt
     3241 ttaacagctg agagttgtaa taccctataa ttttaaacca ctaacctata tatttatgaa
     3301 aaatagaaaa aattaaaacg cattttaagc ctctttcaaa ttaaaagctt catccttatg
     3361 cacaaagagg gcttaaaatg cgttatttta tattgttttt tagtaataat aaaaaaatca
     3421 ctctaattct cccataaata ttatagattc ctaaagatta aaatagatta ttccctattg
     3481 tcttttaagt agcggtagag tgttgccttg ctaataccag tcatttccgt aatttctttt
     3541 acgctataaa cctgactttt atacaatttt actgctcgat caacatcttt ctggttcact
     3601 cgtggacggc cacctttacg acctctcgca cgtgcacttt tcaaaccttc tttggtacgt
     3661 tcaacaatta aatcacgttc gaactgacta aatgcttgga agacagtcat cattaagcgt
     3721 ccgtgaggcg tgctggtatc gaagttttct ttcaagctca ccagcttaac atggtgttct
     3781 tcaaaatagg taacaaggtc aatcaaatcc tttgtactac gacccaatcg agaaaaactt
     3841 tctacaatta ccgtatctcc cggacgaatt ttatctttta aacgatttag ttggggacga
     3901 accgctttgg ttcccgtcat tttctcagtt aggatttcat tacaattttg ttcattgagt
     3961 aagtctaatt gacgatttag ttcttgtgaa cgtgtactca ctcgggcata accataaatg
     4021 tatccactca tgcttttctt cctttcgtac tttaattctg gcttgattgt atcataaaag
     4081 ggagttatag atacatagtt aatgcaacaa gttatgatac aaaatatgac ctcttttatt
     4141 taataaaaga ggtcatttca ttttgtctca taatcgattg tttttgatac gagtgtgtat
     4201 acgaaataaa caaaaaaatc ttaagtaaat ttctagatac ctaatttaat cctaactagt
     4261 gtatcctaaa gttaataacg agtttatcac aatgaaaatt ataaaatgct cccatattag
     4321 atataacatg caatatttga attagaacta caaaatttat tcgttttata aagtaaataa
     4381 tgttgagata ctaaggaata gaatattata aggaattgag ctgaatgtat tggataaaaa
     4441 aagaataact ttattagaga tggactataa aaatgctcgt aattttcttc tgaagtctga
     4501 aagctatttg aatgctcagc taccagaata tattagtttt gacgaaactt taaaatctgc
     4561 taaaaaagtt ttaacttcgc cgtttgtata attaagcgag acaaataaaa aagc
//
LOCUS       DACCCM010000258         4459 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.139, whole genome shotgun sequence.
ACCESSION   DACCCM010000258 DACCCM010000000
VERSION     DACCCM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.139"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            263..1477
                     /locus_tag="IVK78_003076"
     CDS             263..1477
                     /locus_tag="IVK78_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6376441.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1508..1726
                     /locus_tag="IVK78_003077"
                     /pseudo
     CDS             1508..1726
                     /locus_tag="IVK78_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2145..2618
                     /gene="dfrF"
                     /locus_tag="IVK78_003078"
     CDS             2145..2618
                     /gene="dfrF"
                     /locus_tag="IVK78_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6376442.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2745..3401
                     /locus_tag="IVK78_003079"
     CDS             2745..3401
                     /locus_tag="IVK78_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6376443.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3583..4113
                     /locus_tag="IVK78_003080"
     CDS             3583..4113
                     /locus_tag="IVK78_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6376444.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1495 a          656 c          946 g         1362 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgaaaaaa tatctttaaa aataaaaaaa
       61 gacgaaaata aacggttttc tataaaaaga agaccgttta ttttttgtct ttatttgtgt
      121 gaaaaacgaa aaagagcaca acacatcaag cttttattaa ttgtctgtct tttataaatg
      181 caagtattaa ttcaaaaaaa cagaagaaaa aaacaactac accttctttt atactcgtaa
      241 tcgtagaaag gaggcgaaaa aaatgaatac attacctatc aatacaggag atacagcatt
      301 catgatttta tgtacagcaa tggtttgcct gatgactcct ggtctagctt ttttttacgg
      361 cggattagca cggaagaata atatactaac gatcatgtct caatcttttc tctccgttgg
      421 gataacaacg attatgtggg tatttggtgg attcggattg gcctttggac gggatatcgg
      481 cggtgttatt ggaaacccgg cagatttctt tttgatgaga catgttacaa gcttccccaa
      541 catgtttcat ggtgcaacga ttccattttt aatgttcttt ctttaccaac tgatgtttat
      601 cgtgattacc gttcctttga tgacaggagc ttttgcaggg agattgaatt taaaaggcta
      661 tattatactt gttatttttt ggaatctgct gatttatttc cctgtttgcc actggatatg
      721 gggtggcgga tttttggatc agatgggatt tagagatttc gctggtggag cagtcatcca
      781 cacgacagct ggattcggct ctttagcatg tatcctcaca cttggtcaaa gaaagattcc
      841 tttaggagaa agaagtcagc atagcagtct tatggcagct gctatcggta caggactgct
      901 ttggtttggc tggttcggtt ttaattccgg tggtgcactt cgagcagatg aacaagcagt
      961 caatgcattt ggttcaacat tcatcgctct tgcatttgcg atgattactt ggctgatcat
     1021 tgcaaaagta aaaggaaatg gctttgattt tgtcgacgta ctaacaggat ccgttgctgg
     1081 attggcagcc attacgcctt gtgccggtta tgtagaagca aaaagtgcca tgctgattgg
     1141 gatcattgcg ggtatcgtct gtcatgctgc tgttgatttt cgtaaaaaga aacaatggga
     1201 tgatgcactt gatgtgtggg gtgttcatgg catgggcgga ttcacaggta cgattctgat
     1261 agggatcttt gcatctaaca gccatttact aacgaatcca agcagctggt acttcttagg
     1321 gattcaagtg attggtgtca tcattactgc cgtgtatgcc tatgtgctga caacagtgat
     1381 tttaaaatcg gcgatgcact ttacaacgat cactactaca aaagaagaac aagaagaagg
     1441 attggacttt ttcttctttg gcaacaaact caattgaaac aaattcaaat agaaaagagg
     1501 aagattcatg caacaaagca aaacgaaaat tcctgcaaaa aaattaacgt tctttggctt
     1561 tttggctatg accatctcaa tggtcgtttc attatatgaa tatcccacat tcgcgacctc
     1621 aggcttcagc ttagtatttt tcttgttatt aggcggtttg ctatggttta tccctgtggc
     1681 attgtgcgca gcagagatgg caacggtgaa aggatgggaa aaaggtgttt cccaaacaaa
     1741 aagatattaa gaaacagccc atgtttacct gcttgcaaac aaaagaatat tgctactaga
     1801 ctaagagata ctacataagt gtgttggtat ctcttttttg ttgccgtgaa acagcagact
     1861 atttcatttc tgcttgatta ccttaaaact tattagtcta tggtttgtca agggcttgtt
     1921 gcgtgagaaa tgctgaatat caccctttac aaaacatagg ctgataagta acttttcaaa
     1981 agcagaaaag aaatggcaat gcttataaca atatgatata atgcagatga agacaaaaaa
     2041 ggaatttgtc gagctggtta gttcgagata attttgagtt tgttaagatg tggtgcactt
     2101 gatggaatca caggtacgac atcatattat ggaggatgaa atagatgata ggtttgattg
     2161 ttgcgaggtc aaagaataat gttataggca agaatggtaa tataccatgg aaaataaagg
     2221 gagaacaaaa gcaatttaga gagttaacaa cgggtaatgt ggttattatg gggcgaaagt
     2281 cttatgaaga aatcggtcat ccgttgccta atagaatgaa tattgttgtt tccaccacaa
     2341 cagagtatca aggagataat ttagtttcag ttaaatcatt agaagatgca ttattattgg
     2401 ctaaaggacg agatgtatac atatctggtg gatatggact atttaaggaa gctttgcaaa
     2461 tagtagataa aatgtatatc acagaagtag atttaaatat tgaagatgga gatacattct
     2521 ttccagaatt tgatatcaat gattttgaag ttttgatagg ggaaacactt ggtgaggaag
     2581 tgaaatatac gagaacattt tatgtaagga aaaaatgaat tgagtaaatt ttggatttag
     2641 ggtgttttca taaatatatt gctttttatg catatataaa ttgttgtaag acaaattcca
     2701 gtttgtatgg ggcgataaaa tcagaatttg agagaggatt gaatatgaat aaggaatggt
     2761 ctgaacttaa taaaacaatg caggcacaaa taaaaaagaa agatacttat aagaggggta
     2821 ttgatacttt acttacttta cgaagtcagt taatacaaac cttagtatct ttcaaagaag
     2881 aattatgcag agaagatttt aactcaatcc ccttcataaa cgctgatggt tatcatagta
     2941 agacgattgc ttattctatt tggcatattt tccgcattga agatatagtt gtgcatacag
     3001 tgataaatga agatgaacaa gtattttttg caggcaatta tcaagagcgt atcaattcac
     3061 ctatcattac aacaggaaat gaactcatga aacagcagat tgcagacttt tcaaaacagc
     3121 ttaatctgga agaattatat ttatatattt tcgaagtatg ggaaagcact gagaaaatgc
     3181 ttgaacggtt atcttatgat gaattgaaaa gaaaaatacc gaaagaaaga aaaggatact
     3241 tagaatcgtt gaatgtagta aacgacaatg aaaaagcaat ttggctgatt gattattggt
     3301 gtaataaaga tatttgtgga ctaattcaaa tgccgttttc aagacattgg attatgcaca
     3361 cagaagcctg tttgcgtata aagaacaaga tacattcata ggcaaaaatt tcagtttgta
     3421 gaactaagga aattggaatt taaggaggaa taaagtatgg ttagtgattt tataagagca
     3481 gcatttccat ggattttaat gggtttattt gtagcaatca gttgttccct tatgagtaaa
     3541 aaagagaaat aaattcctgt ttgtatttat aaaagagaaa aaatgagaaa agtagtttta
     3601 tttattgcca tgagccttga cggatatatt gcggacggta atggtggagt agcttggcta
     3661 aatggtcatg gaaatgacaa tgaaaacatc gacacttata cagaatttac caaagatata
     3721 gatactgtct taatggggtg gaatacttct catcaggttg ttactgaact ttctccgcaa
     3781 gaatgggtat ataataaatt tacaacttat gtattaacac ataaagagtg caattcttcc
     3841 caacaaattc attttacaag tgaaaatcct gttttattat tggaacggct aaaacaagaa
     3901 gcaggaaagg atatttggat ttgtggcgga gcaaatcttg ttcagcagtt ggtaagcaaa
     3961 aatatgattg ataaatatta catttctgtt attcctactc tgttaggaaa tggggttcgt
     4021 cttttgggca acatagacag ggaaataaag ttaaggcttt gtaaaacaca gacttataac
     4081 ggaataaccg atttgattta tatgcgtaga taactagaga gaaacagaaa agctgttaaa
     4141 ccgacggagc gatccgacca cgttggcggg aaaatcctta agatttttcc gccatgcttg
     4201 caagggggcg gttcgcttcg gcggttcggc gagcttgcga gccgttcaaa gcccccgtta
     4261 tctaacaagg ggggcacaaa aaacaagaaa cactatacac taacaagcgg aaaacgctgt
     4321 atttataagg caaaacccca tttttacagt agtgatataa attgtaccta tatcactatg
     4381 aaaattgaat agtaaatatg agaaagacag ttgatttcaa aaaataggct ctttgtcaaa
     4441 tgatgttggt aaagagaaa
//
LOCUS       DACCCM010000259         4148 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.147, whole genome shotgun sequence.
ACCESSION   DACCCM010000259 DACCCM010000000
VERSION     DACCCM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.147"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            109..192
                     /locus_tag="IVK78_003081"
     CDS             109..192
                     /locus_tag="IVK78_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6376445.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK78_003082"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK78_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6376446.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IVK78_003083"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IVK78_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IVK78_003084"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IVK78_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IVK78_003085"
     CDS             2348..2593
                     /locus_tag="IVK78_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6376447.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IVK78_003086"
     CDS             2696..3565
                     /locus_tag="IVK78_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6376448.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..>4148
                     /locus_tag="IVK78_003087"
     CDS             3546..>4148
                     /locus_tag="IVK78_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6376449.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKALCQMMLVKRNL"
BASE COUNT         1478 a          609 c          800 g         1261 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aggctctttg tcaaatgatg ttggtaaaga
     4141 gaaatctt
//
LOCUS       DACCCM010000260         3256 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.160, whole genome shotgun sequence.
ACCESSION   DACCCM010000260 DACCCM010000000
VERSION     DACCCM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.160"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            244..1212
                     /locus_tag="IVK78_003088"
     CDS             244..1212
                     /locus_tag="IVK78_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6376450.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1205..2236
                     /gene="vanA"
                     /locus_tag="IVK78_003089"
     CDS             1205..2236
                     /gene="vanA"
                     /locus_tag="IVK78_003089"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6376451.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2242..2850
                     /locus_tag="IVK78_003090"
     CDS             2242..2850
                     /locus_tag="IVK78_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6376452.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          967 a          623 c          789 g          877 t
ORIGIN      
        1 tgctggtgtc atccaccaac atcaaaaagt atagagccat aattttttag gaaaatctca
       61 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
      121 tacacggtag acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga
      181 caagattacc attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta
      241 attatgaata acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc
      301 catgctcttt cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa
      361 tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt
      421 tccgcctcta ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc
      481 atcggctgca atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat
      541 gtggcgtact cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta
      601 cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac
      661 cgtggcaagg tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa
      721 gcggttattg agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga
      781 agtatagagg taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg
      841 cttcatgtgc cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga
      901 atgaagcaag gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag
      961 ttggttaaag cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga
     1021 gaggaagagt ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt
     1081 aaacttcaaa gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa
     1141 gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag
     1201 gagcatgaat agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt
     1261 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
     1321 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
     1381 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
     1441 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
     1501 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
     1561 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
     1621 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
     1681 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
     1741 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
     1801 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
     1861 attgggaaac agtgccgcgt tagctgttgg cgaggtggac caaatcaggc tgcagtacgg
     1921 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
     1981 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
     2041 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
     2101 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
     2161 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
     2221 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
     2281 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
     2341 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
     2401 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaat
     2461 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
     2521 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
     2581 tcaagccata gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag
     2641 cttgtaccaa tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat
     2701 ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt
     2761 gggtttgaag catatagcct cgaatggtgg cactatgtat taagagacga accatacccc
     2821 aatagctatt ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat
     2881 aagctaactc tttcggcagg aaacccgacg tatgtaactg gttcttaggg aatttatata
     2941 tagtagatag tattgaagat gtaaggcaga gcgatattgc ggtcattatc tgcgtgcgct
     3001 gcggcaagat agcctgataa taagactgat cgcatagagg ggtggtattt cacaccgccc
     3061 attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg gtatcactta tgaaaattca
     3121 tctacattgg tgataatagt aaatccagta gggcgaaata attgactgta atttacgggg
     3181 caaaacggca caatctcaaa cgagattgtg ccgtttaagg gctctttgtc aaatgatgtt
     3241 ggtaaagaga aatctt
//
LOCUS       DACCCM010000261         2246 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-11-S
            SAMN13750723-rid10751983.guided.174, whole genome shotgun sequence.
ACCESSION   DACCCM010000261 DACCCM010000000
VERSION     DACCCM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750723
            Sequence Read Archive: SRR10270773
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860347.1
            Genome Coverage       :: 501x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 21:02:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750723-rid10751983.guided.174"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-11-S"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..152
                     /locus_tag="IVK78_003091"
     CDS             <1..152
                     /locus_tag="IVK78_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6376453.1"
                     /translation="YLIVDLKLCNRTNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
     gene            366..1061
                     /gene="vanR"
                     /locus_tag="IVK78_003092"
     CDS             366..1061
                     /gene="vanR"
                     /locus_tag="IVK78_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6376454.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1039..2193
                     /locus_tag="IVK78_003093"
     CDS             1039..2193
                     /locus_tag="IVK78_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6376455.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          758 a          410 c          475 g          603 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa ttatgcggta aagctatata
       61 aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat
      121 tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata
      181 tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc
      241 cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc
      301 ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga
      361 taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg
      421 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
      481 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
      541 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
      601 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
      661 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
      721 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
      781 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
      841 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
      901 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
      961 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
     1021 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
     1081 ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg
     1141 ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa
     1201 aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac
     1261 aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc
     1321 atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt
     1381 cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc
     1441 aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga
     1501 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
     1561 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
     1621 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
     1681 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
     1741 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
     1801 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
     1861 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
     1921 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
     1981 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
     2041 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
     2101 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
     2161 atgccagact tggttgataa aaggaggtcc taagagatgt atataatttt ggctctttgt
     2221 caaatgatgt tggtaaagag aaatct
//
